Microfluidic flow cell, production method, use and analysis device

Abstract

The invention relates to a microfluidic flow cell (1a), a production method for a microfluidic flow cell, use of a microfluidic flow cell, and an analysis device. To permit simple production of the flow cell (1a) and a particularly good mixing of a dry substance (10a), arranged in a cavity (9), with a fluid, a passage (7) opens into the cavity (9) and an element (2a) inserted in the passage (7) delimits the cavity (9) by means of a deformable wall (12a).

Description

[0001] Description:

[0002] Microfluidic flow cell, manufacturing process, use and analysis device

[0003] The invention relates to a microfluidic flow cell, a manufacturing method for a microfluidic flow cell, a use of a microfluidic flow cell and an analysis device.

[0004] Microfluidic flow cells, which are increasingly being used as "mini-laboratories" for the analysis and / or synthesis of fluids, particularly in diagnostics, often contain a dry substance that must be introduced into the flow cells during their manufacture. To introduce a dry reagent, a reagent liquid to be dried, i.e., a carrier liquid with a reagent dissolved or suspended therein, is brought to the relevant location in an assembly step in which the cavity within the flow cell intended for the dry reagent, e.g., a channel or chamber, is still accessible.The entire flow cell component, which is only partially wetted with the reagent fluid, must then be subjected to a drying process before further assembly. This drying process is often combined with heat treatment to accelerate the process or, to protect the reagents and ensure stability and resuspension properties, is performed as a freeze-drying process. The disadvantage is that the flow cell, whose dimensions usually far exceed those of the area to be dried, takes up a lot of space in a drying chamber. Furthermore, the drying process can damage the flow cell itself, especially sensitive components mounted on it.Furthermore, during the final assembly of the flow cell, the dry substance formed may be subject to adverse effects from air contact, particularly air humidity, and welding heat, or from the influence of adhesives used during assembly, which in many cases require the corresponding channel regions of a microfluidic flow cell to be hermetically sealed. A method for introducing a dry substance into a flow cell, as described above, is disclosed, for example, in EP 2 198 964 B1. It is also known through practice to prefabricate dry substance as lyophilized (freeze-dried) balls or spheres and then introduce them into the flow cell.

[0005] The invention is based on the object of creating a flow cell of the type mentioned above that is easy to manufacture and enables particularly good mixing of the dry substance with a fluid. The flow cell according to the invention that solves this problem is characterized in that a passage opens into the cavity, and an element inserted into the passage with a deformable wall delimits the cavity. The dry substance can be introduced into the cavity particularly easily through the passage, and the deformable wall adjacent to the cavity enables the cavity to be modified and thus the dry substance arranged therein to be influenced from the outside, as will be described in more detail below.

[0006] The dry substance is preferably a dry reagent in the form of so-called beads, balls, or pellets, which can be produced by lyophilization (freeze-drying) and in particular have a diameter of 2 to 5 mm. Thus, the dry substance is often larger than an adjacent channel of the flow cell. In an advantageous embodiment, the deformable wall can be concave in sections and enclose a part of the cavity, which is in particular in the shape of a cylinder or a spherical segment.

[0007] As a result, a larger volume of dry substance can be arranged close to the deformable wall and influenced by the deformable wall. In addition, a relatively large change in volume can be achieved upon movement of the deformable wall, which in particular offers the possibility of minimizing the dead volume and thus air inclusions and also realizing a pumping effect for transporting the supplied fluid. In a particularly advantageous embodiment, the dry substance can be arranged on an inner side of the deformable wall facing the cavity, in particular on a concave section of the deformable wall. Further advantageously, the deformable wall can at least partially enclose the dry substance in order to hold the dry substance in a frictional and / or positive-locking manner.This allows the dry substance to be positioned at a defined position in the flow cell and also facilitates the fabrication of the flow cell, as described below.

[0008] The inserted element can have a recess on an outer side facing away from the cavity, which recess can, for example, be cylindrical and preferably borders the deformable wall. When using the flow cell, components described in more detail below can protrude into the recess to move the deformable wall and / or act on the dry substance arranged in the cavity.

[0009] The inserted element can preferably be made in one piece, particularly from silicone, thermoplastic elastomer (TPE), or thermoplastic polyurethane (TPU), and can be formed, in particular, by injection molding. This enables cost-effective prefabrication of the element to be subsequently inserted into the flow cell.

[0010] Furthermore, a method for producing the aforementioned microfluidic flow cell is described, which is characterized in that the dry substance is introduced into the hollow chamber through the passage. This allows the flow cell to be largely completed without dry substance during production before the dry substance is introduced in a final step and the passage is closed by means of the inserted element.

[0011] This makes it possible to exclude, in particular, any adverse influence on the dry substance during the production of the flow cell.

[0012] A particularly simple manufacturing process can be achieved by first connecting the dry substance to the element to be inserted and then inserting it into the passage together with the element. The element to be inserted protects the dry substance from external influences until the element with the dry substance is inserted into the flow cell. Furthermore, the insertion of the element with the dry substance can be performed particularly easily by machine, as the element to be inserted has a defined shape and can also be easily gripped by machine.

[0013] Particularly advantageously, the dry substance can be held in the inserted element in a frictional and / or positive-locking manner. This is achieved by the dry substance elastically deforming the deformable wall of the inserted element. If the geometry of the inserted element, and in particular the deformable wall, is adapted to the dry substance in such a way that the dry substance is engaged behind it, the dry substance is also held in a positive-locking manner. This makes it particularly easy to prefabricate and store the inserted element with the dry substance, preventing any unintentional detachment of the dry substance from the inserted element before assembly on the flow cell.

[0014] Additionally or alternatively, the dry substance can be held magnetically and / or electrostatically when inserted into the cavity. An additional magnetically active element comprising a permanent magnet and / or an electromagnet can be provided.

[0015] Furthermore, the use of the aforementioned microfluidic flow cell for analyzing a sample is claimed, which is characterized in that the deformable wall is moved in order to distribute the dry substance in a fluid supplied into the cavity. The deformable wall makes it particularly easy to exert mechanical force on the dry substance and / or the fluid arranged in the cavity, thereby accelerating the distribution of the dry substance in the fluid. The deformable wall can also be moved protruding into the channel, thereby reducing the channel cross-section and assisting the distribution of the dry substance. Alternatively or additionally, the dry substance can also comprise magnetically active components which are magnetically acted upon by the deformable wall in order to distribute the dry substance in a supplied fluid.The magnetic application of a dry substance in a flow cell represents a unique invention, independent of the presence of an inserted element and a deformable wall. Particularly advantageously, the deformable wall can be moved, preferably elastically, to such an extent that the dry substance is pressed against a wall of the flow cell and thus crushed. This mechanical reduction of the dry substance also accelerates its distribution in the supplied fluid.

[0016] The deformable wall can be moved successively in different, preferably opposite directions to alternately increase and decrease the volume of the cavity. This can achieve a pumping effect that moves the supplied fluid within the hollow chamber and the at least one adjacent channel, which additionally accelerates the distribution of the dry substance in the fluid. It can be particularly advantageous that the fluid is only supplied into the cavity after the cavity has been reduced in size by the deformable wall. This avoids unnecessary dead spaces and thus, for example, air accumulation within the cavity. The supply of the fluid can be achieved by elastic recovery of the deformable wall and the resulting negative pressure in the hollow chamber.Alternatively or additionally, a vacuum can be applied to the outside of the deformable wall, which retracts the deformable wall.

[0017] Furthermore, an analysis device for analyzing a sample by means of the aforementioned microfluidic flow cell is also claimed, which is characterized in that an operating device is provided for moving the deformable wall. The operating device can advantageously have a movable plunger and / or a tube for applying negative and / or positive pressure in order to move the deformable wall. In addition, the analysis device can comprise at least one magnetically active element which acts on the dry substance. For example, magnetically active components such as metal parts can be provided in the dry substance, which can be magnetically held or moved in order to hold the dry substance in a specific place or to promote the distribution of the dry substance in the fluid. The analysis device can have at least one inlet and / or outlet line to or from the dry substance.from the flow cell for a sample and / or at least one sensor for determining the location of a fluid or a sample in the flow cell or for analyzing a sample prepared in the flow cell. The inlet and / or outlet line can be pressed against the flow cell to create a hermetically sealed connection. Reference is made to LU502668, the content of which is hereby incorporated into this application. This document shows further embodiments and uses of the flow cell according to the invention with an inserted element (referred to therein as a reagent plug, RP for short) and a movable wall (referred to therein as a flexible wall, FW for short) as well as a corresponding analysis device. Further special features and advantages of the invention emerge from the following description of preferred exemplary embodiments with reference to the drawings. Therein:

[0018] Fig. 1 shows a longitudinal section through a flow cell with an inserted element and a dry substance according to a first embodiment;

[0019] Fig. 2 shows a longitudinal section through a flow cell with an inserted element and a dry substance according to a second embodiment;

[0020] Fig. 3 shows a longitudinal section through a flow cell with an inserted element without dry substance according to a third embodiment;

[0021] Fig. 4 shows a longitudinal section through the flow cell shown in Figure 3 with a dry substance;

[0022] Fig. 5 shows a longitudinal section through a flow cell with an inserted element and a dry substance according to a fourth embodiment;

[0023] Fig. 6 shows a longitudinal section through the flow cell of Fig. 5 with an additional magnet;

[0024] Fig. 7 shows a longitudinal section through the flow cell of Figure 5 with a supplied fluid;

[0025] Fig. 8 is a longitudinal section through the flow cell of Figure 7 with a pneumatic operating device;

[0026] Fig. 9 is a longitudinal section through the flow cell of Figure 7 with a mechanical operating device;

[0027] Fig. 10 shows a longitudinal section through the flow cell of Fig. 1 with a supplied fluid and an operating device in a first actuating position;

[0028] Fig. 1 1 shows a longitudinal section through the flow cell of Figure 1 with the operating device in a second actuating position;

[0029] Fig. 12 shows a longitudinal section through the flow cell of Figure 7 with an operating device and Fig. 13 shows the sequence of three steps in the prefabrication of the unit from the element to be inserted and the dry substance.

[0030] Figure 1 shows a longitudinal section through a flow cell 1a with an inserted element 2a according to a first embodiment. The flow cell 1a comprises, in a known manner, a substantially planar, flat substrate 3 which is covered on its underside by a film 4. The film 4 is preferably welded or glued to the substrate 3. At least one channel 5, which is delimited by the film 4, is provided in the substrate 3. Such a flow cell 1a can be manufactured particularly easily by pre-injection-molded plastics material into the substrate and then covering the outwardly open channel 5 with the film 4 to enable reliable supply and removal of liquid within the channel 5 through an inlet and an outlet.The substrate 3 can be made of plastic such as PP, PC, PMMA, COC, COP or PS and the inserted element 2a can be made of a thermoplastic elastomer such as PU, TPE, silicone or vice versa. In a region of the substrate 3 (not shown), a detection region for analyzing a mixture of sample and dry substance can be provided. On the side of the substrate 3 facing away from the film 4, the substrate 3 comprises a protruding, preferably annular bushing 6, through which an outwardly open passage 7 opens into the channel 5. The element 2a is inserted into the passage 7 from the outside, with a laterally protruding annular collar 8a resting on the bushing 6 and thereby forming a depth limiter for the element 2a inserted into the bushing 6. Additionally or alternatively, a depth stop can also be implemented on the passage.The element 2a preferably defines a cavity 9 in the passage 7, which also extends into the inserted element 2a, where a spherical dry substance 10a is arranged. An annular groove 11a is provided around the cavity 9 in the upper region on an outer side of the inserted element 2a, which is spaced from the cavity 9 by a thin wall. The contour of the inserted element 2a results in a roughly W-shape in the longitudinal section shown. The wall 12a of the inserted element 2a, which rests against the dry substance 10 and surrounds it, is deformable, for example from an elastic material such as silicone, TPE or TPU, whereby the dry substance 10a can be subjected to a force from the outside particularly easily. The walls of the inserted element 2a are arranged at right angles to one another in the longitudinal section.In the cross-section, which runs horizontally in Figure 1, the inserted element 2a, as in the other figures, preferably has a circular, rotationally symmetrical shape.

[0031] In the embodiment of Figure 1 and the further embodiments described below, the surfaces of the inserted element facing the cavity and / or the surface of the channel can be hydrophilically modified, for example non-permanently by surface activation using plasma treatment, or coated (permanently, e.g., by plasma polymerization or coating with suitable liquids) to support the introduction of samples and / or the redissolution of the dry substance. The pretreatment for hydrophilic surface properties can include a contact angle to water of < 60°, preferably < 30° (wet-chemical, plasma treatment, corona, coating with a glassy layer, e.g., by plasma polymerization, or with metal, e.g., by printing or thin-film technology). In addition, the surface of the inserted element can preferably be flush with one of the walls of the channel.

[0032] Figure 2 shows a longitudinal section through a flow cell 1b with an inserted element 2b and a dry substance 10b according to a second embodiment. As in the following figures, the substrate 3 with the

[0033] Bushing 6 as well as the channel 5 with the passage 7 and the foil 4 are of identical design, so that the same reference numerals are used. As can be seen from the comparison with the embodiment of Figure 1, the embodiment of the inserted element 2b shown in Figure 2 differs in particular in that in the longitudinal section the deformable wall 12b, which is

[0034] Dry substance 10b is curved, in particular in the shape of a circular segment, and thus lies fully against the spherical dry substance 10b. In order to ensure the depth of the inserted element 2b in the passage 7, the element 2b also comprises a laterally projecting annular collar 8b. A corresponding annular groove 11b is formed according to the curved course of the deformable wall 12b. In addition, an annular seal 13 is shown, which is arranged in an annular groove on the end face of the inserted element 2b facing the substrate 3 and enables a reliable seal between the substrate 3 and the inserted element 2b. Such an annular seal can also be used in the embodiments shown in the other figures

[0035] Embodiments of the inserted element can be provided, even if this is not shown in all figures for the sake of simplicity. Figures 3 and 4 each show a longitudinal section through a flow cell 1c according to a third embodiment, Figure 3 showing the flow cell 1c with an inserted element 2c without dry substance and Figure 4 showing it with dry substance 10c. As can be seen from a comparison of Figures 3 and 4, the deformable wall 12c of the element 2c is originally flat and is delimited on the outside by a cylindrical recess 14 in the inserted element 2c. The position of the deformable wall 12c shown corresponds to the state when a previously present dry substance has been completely dissolved in a supplied fluid and discharged through the channel 5.In Figure 4, however, the flow cell 1c is shown after production with the inserted element 2c and the dry substance 12c arranged in the cavity 9. The dry substance 12c rests against the film 4 and presses against the deformable wall 12c, which preferably bulges outwardly, elastically, in the direction of the recess 14a. This prestressing facilitates the mechanical comminution of the dry substance 10c by an additional external force acting on the deformable wall.

[0036] The force acting on wall 12c is reduced and the dead volume is reduced, which also means that less fluid is required.

[0037] Figures 5, 6, and 7 show a flow cell 1d with an inserted element 2d and a dry substance 10d in the form of a plurality of beads according to a fourth embodiment. The individual beads of the dry substance 10d can be magnetically influenced, i.e., they comprise either a magnetic component, such as a permanent magnet, or a magnetizable component, such as a metal. The inserted element 2d, which can be identical to the embodiment shown in Figures 3 and 4, holds the dry substance 10d formed from beads to the deformable wall 12d in the cavity 9 (not numbered here), either through magnetic forces or in another way, e.g., through adhesion or a removable adhesive, after the flow cell 1d has been manufactured.Some of the spheres of the dry substance 10d also protrude into the channel 5, which is advantageous for the subsequent distribution and dissolution of the dry substance 10b in a supplied fluid.

[0038] Figure 6 also shows a magnet 15, which projects into the recess 14b and rests on the outside of the deformable wall 12d opposite the dry substance 10d. The magnet 15, which can be designed as a permanent magnet or electromagnet, can exert a magnetic force on the dry substance 10d, in particular an attractive and / or repulsive force. This can assist the distribution of the dry substance 10d in a supplied fluid, and in addition, components of the dry substance 10d that are not dissolved in the fluid can be collected and held again by the magnet 15. The magnet 15 can additionally be pressed against the deformable wall 12d in order to cause movement of the deformable wall 12d and thus of the dry substance 10d and a supplied fluid, which additionally assists the distribution of the dry substance in the fluid.In addition, the channel cross-section is reduced by a wall 12d deformed in the direction of the channel, which also increases the magnetic effect in the channel 5.

[0039] Figure 7 shows one possibility of distributing or dissolving the dry substance 10d, which is in the form of small beads, by moving a fluid 16 arranged in the channel 5. The fluid 16 can preferably be moved back and forth periodically, for example by an overpressure applied alternately to the ends of the channel 5. In addition, as shown in Figure 7, a magnet 15 can also be provided, which acts on the dry substance 10d magnetically and, if appropriate, by movement. Figures 8 and 9 show the flow cell 1d of Figure 7 in longitudinal section with various operating devices. In the embodiment shown in Figure 8, a pneumatic operating device 17a is provided, which bears sealingly against the inserted element 2d, in particular against the annular collar 8d, and is designed to generate a negative and / or positive pressure in the recess 14b.Because the recess 14b is hermetically sealed by the pneumatic operating device 17a, positive and / or negative pressure leads to the movement of the deformable wall 12d indicated by the arrow, whereby the dry substance 10d, formed as a sphere, is distributed particularly well in the fluid 16. If the negative and / or positive pressure is applied periodically, the deformable wall 12d and thus also the fluid 16 can be set into vibration, which additionally supports the distribution of the dry substance 10d.

[0040] In Figure 9, a mechanical operating device 17b is provided, which is designed, for example, as a plunger and protrudes into the recess 14b against the deformable wall 12d. When the mechanical operating device 17b moves in the direction of the deformable wall 12d, the deformable wall 12d is deflected, thereby also transmitting a movement to the dry substance 10d and the fluid 16. The movement of the mechanical operating device 17b can also occur periodically to additionally support the distribution of the dry substance 10d in the fluid 16. Figures 10 and 11 show the flow cell 1a of Figure 1 with a supplied fluid and an operating device 17c in different actuating positions.In the actuating position shown in Figure 10, the operating device 17c shown schematically here, which can be a pneumatic or mechanical operating device, is provided to act on the deformable wall 12a in the direction of the dry substance 10a.

[0041] Here, the deformable wall 12a as well as the originally spherical dry substance 10a are already deformed. From a certain degree of deformation, the dry substance 10a breaks into several small pieces, which are particularly well distributed or dissolved in the fluid 16. This state is shown in Figure 11, wherein the deformable wall 12a completely fills the passage 7 and the cavity 9, so that only the channel 5 with the fluid 16 arranged therein remains and the dry substance 10a is completely distributed or dissolved in the fluid 16. Figure 12 shows a longitudinal section through the flow cell of Figure 7 with an operating device 17d. As can be seen there, a pipe 18, shown here only in sections, is sealingly connected to the inserted element 2b, in particular to the annular collar 8d, through which a negative and / or positive pressure can be introduced into the recess 14d in order to move the deformable wall 12d as previously described.Additionally, a rod-shaped magnet 19, for example, is preferably provided concentrically in the tube 18. This magnet extends into the recess 14b and rests against the outer side of the deformable wall 12d. This allows magnetic components of the dry substance 10d to be held and also moved by the negative and / or positive pressure that can be introduced into the tube 18.

[0042] Figure 13 shows the sequence of three steps in the prefabrication of a unit consisting of the element to be inserted, here using the example of the element 2b to be inserted, and the dry substance, here using the example of the dry substance 10b. As can be seen from the left-hand illustration, in a first step the dry substance 10b is inserted into the cavity 9 within the element 2b and held there preferably by form-fitting and / or frictional engagement. A prefabricated unit of this type can be seen in the middle illustration, which can also be stored without any problems. The right-hand illustration shows the final step, in which the prefabricated unit is inserted into the passage 7 within the bushing 6 of the flow cell.This prefabrication also reliably prevents contamination of the dry substance 10b and mechanical stress during storage and transport, since the dry substance 10b is not gripped and held directly, but exclusively via the element 2b.

[0043] The embodiments shown in the various figures can be easily combined. For example, the various operating devices can also be provided on the other elements used. The various elements used and the different operating devices can therefore be combined in any variation.

[0044] List of reference symbols: 1 a, 1 b, 1 c, 1 d flow cell

[0045] 2a, 2b, 2c, 2d Inserted element

[0046] 3 Substrat

[0047] 4 slides

[0048] 5 channel

[0049] 6 socket

[0050] 7th round

[0051] 8a, 8b, 8c, 8d ring bundle

[0052] 9 Cavity

[0053] 10a, 10b, 10c, 10d dry matter

[0054] 1 1 a, 1 1 b annular groove

[0055] 12a, 12b, 12c, 12d Wall

[0056] 13 Ring seal

[0057] 14a, 14b recess

[0058] 15 Magnet

[0059] 16 Fluid

[0060] 17a, 17b, 17c, 17d Operating equipment

[0061] 18 pipe

[0062] 19 Magnet

Claims

Patent claims:

1. Microfluidic flow cell (1 a; 1 b; 1 c; 1 d), with a dry substance (10a; 10b; 10c; 10d) arranged within the flow cell (1 a; 1 b; 1 c; 1 d) in a cavity (9) for interaction with a fluid (16) that can be fed into the cavity (9), characterized in that a passage (7) opens into the cavity (9) and an element (2a; 2b; 2c; 2d) inserted in the passage (7) with a deformable wall (12a; 12b; 12c; 12d) delimits the cavity (9).

2. Flow cell (1 a; 1 b) according to claim 1, characterized in that the deformable wall (12a; 12b) is at least partially concave and encloses at least part of the cavity (9).

3. Flow cell (1 a; 1 b) according to claim 1 or 2, characterized in that the deformable wall (12a; 12b) encloses a part of the cavity (9), which is designed in particular in the form of a cylinder or a spherical section.

4. Flow cell (1 a; 1 b; 1 c; 1 d) according to one of the preceding claims, characterized in that the dry substance (10a; 10b; 10c; 10d) is arranged on an inner side of the deformable wall (12a; 12b; 12c; 12d) facing the cavity (9), in particular on a concave section of the deformable wall (12a; 12b). 5, flow cell (1 a; 1 b) according to claim 4, characterized in that the deformable wall (12a; 12b) at least partially encloses the dry substance (10a; 10b).

6. Flow cell (1 a; 1 b; 1 c; 1 d) according to one of the preceding claims, characterized in that the inserted element (2a; 2b; 2c; 2d) is arranged on a surface adjacent to the cavity (9) facing away side has a recess (14a; 14b) which is in particular cylindrical in shape.

7. Flow cell (1 a; 1 b; 1 c; 1 d) according to one of the preceding claims, characterized in that the inserted element (2a; 2b; 2c; 2d) consists of silicone, TPE, TPU and is formed in particular by injection molding.

8. A method for producing a microfluidic flow cell (1a; 1b; 1c; 1d) according to one of claims 1 to 7, characterized in that the dry substance (10a; 10b; 10c; 10d) is introduced into the cavity (9) through the passage (7).

9. A method according to claim 8, characterized in that the dry substance (10a; 10b; 10c; 10d) is first brought into contact with the element (2a; 2b; 2c, 2d) and then inserted into the passage (7) together with the element (2a; 2b; 2c, 2d).

10. Method according to claim 9, characterized in that the dry substance (10a; 10b; 10c; 10d) is held in the element (2a; 2b; 2c, 2d) by friction and / or form-fitting. 1 1. Method according to one of claims 8 to 10, characterized in that the dry substance (10a; 10b; 10c; 10d) is held magnetically and / or electrostatically when inserted into the cavity (9).

12. Use of a microfluidic flow cell (1 a; 1 b; 1 c; 1 d) according to one of claims 1 to 7 for analyzing a sample, characterized in that the deformable wall (12a; 12b; 12c; 12d) is moved in order to distribute the dry substance (10a; 10b; 10c; 10d) in a fluid (16) supplied to the cavity (9).

13. Use according to claim 12, characterized in that the deformable wall (12a; 12b; 12c; 12d) is moved so far that the dry substance (10a; 10b; 10c; 10d) is pressed against a wall (4) of the flow cell (1a, 1b; 1c; 1d) and is thus comminuted.

14. Use according to claim 12 or 13, characterized in that the deformable wall (12a; 12b; 12c; 12d) is moved successively in different directions in order to alternately increase and decrease the volume of the cavity (9).

15. Use according to one of claims 12 to 14, characterized in that the fluid (16) is only fed into the cavity (9) after the cavity (9) has been reduced in size by the deformable wall (12a; 12b; 12c; 12d).

16. Analysis device for analyzing a sample by means of a microfluidic flow cell (1 a; 1 b; 1 c; 1 d) according to one of claims 1 to 7, characterized by an operating device (17a; 17b; 17c; 17d) for moving the deformable wall (12a; 12b; 12c; 12d).

17. Analysis device according to claim 16, characterized in that the operating device (17a; 17b; 17c; 17d) comprises a movable plunger (17b; 17c; 19) and / or a tube (18) for applying a negative and / or positive pressure in order to move the deformable wall (12a; 12b; 12c; 12d).

18. Analysis device according to claim 16 or 17, characterized by a magnetically active element (19) which acts on the dry substance (10a; 10b; 10c; 10d).

Images