Fungal biomass, method for the preparation and uses thereof, and edible compositions comprising said fungal biomass

Abstract

Disclosed is a fungal biomass comprising one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae, wherein the fungal biomass has high crude protein and high content of one or more polyunsaturated fatty acids. Further, an edible composition comprising the fungal biomass is disclosed. A method for the continuous preparation of a fungal biomass using one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae is also disclosed, and further use of the fungal biomass and the edible composition as or in foodstuff is disclosed.

Description

[0001]FUNGAL BIOMASS, METHOD FOR THE PREPARATION AND USES THEREOF, AND EDIBLE COMPOSITIONS COMPRISING SAID FUNGAL BIOMASS TECHNICAL FIELD The present disclosure relates to fungal biomasses and methods for the preparation, edible compositions comprising said biomass, and uses thereof. More particularly, the present disclosure relates to a fungal biomass comprising filamentous fungi of the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae, wherein the fungal biomass has a high crude protein content. BACKGROUND Biorefinering becomes increasingly important with an in- creasing population and the need to combat climate change. The growing demand for food and sustainable food production are global challenges, and the lack of sustainably produced protein is a significant part of the problem. Production of protein source, which may be used as or in foodstuff, such as animal feed (fodder) and compound feed, has constantly increased in response to the rapidly increasing global demand. However, current production of many protein sources, such as meat, is not sustainable and / or ecological. Driven by ethical and environmental concerns, the demand for new alternative protein and fat sources is growing rapidly both for feed and human nutrition. In addition to plant-based sources, algae and insects, microbial proteins and fats produced in a bioreactor, i.e., a fermentor, are one of the most potential future sources of these nutrients. When the whole cultivated mi- crobial biomass is used as a protein component, it is referred to as single cell protein (SCP). When the microbial species used in SCP belongs to the fungal organisms, the term mycoprotein is also used. A well-known example of mycoproteins in commercial produc- tion is Quorn. One of the factors limiting the wider use of mycoproteins is a lower protein content in the products compared to plant-based protein concentrates (~65% protein) and isolates (~85% protein). Another limiting factor for wider use of mycoproteins is low con- centration of polyunsaturated fatty acids in the mycoprotein. Fish feed containing fishmeal and fish oil as key compo- nents has widely been used since the 1970s. However, the production and comprehensive use of fishmeal are controversial since it may lead to environmental damage, depletion of ecosystems, and the collapse of local fisheries. Soy protein concentrate (SPC) has commonly been used in modern fish feed in aquaculture. SPC is made from soybean and contains about 70% crude protein, which can be used as the protein source in fish feed. However, soy cultivation poses ecological challenges such as deforestation in South America and therefore, there is a demand for alternatives for SPC that are sustainable produced. Proteinaceous substances suitable for use as fodder and foodstuffs have been manufactured by submerged aerobic cultures of species of mycelium-growing micro-organisms (patent FI44366B). Among processes disclosed in patent FI44366B are those wherein the micro-organism Paecilomyces varioti was cultivated in spruce cal- cium bisulphite spent liquor in a continuous cultivation process. A maximum protein content of 56.8% is disclosed when using Paeci- lomyces varioti. In addition, agricultural waste has been used by the com- pany BioTork as a substrate for algae and fungi to produce fish feed components. Furthermore, biomass consisting of microalgae Nannochlo- ropsis oculata and whole cells of DHA-rich Schizochytrium sp. for fish-free aquaculture feed has been disclosed (Sarker et al., Sci Rep. 2020; 10: 19328). Different fermentation methods for mycoprotein production include batch, fed-batch, and continuous fermentation. In batch fermentation, microorganisms are inoculated to a fixed volume of medium in a fermentor. With microbial growth, the nutrients are gradually consumed. The broth is removed at the end and biomass is harvested. Fed-batch fermentation is a modified version of batch fermentation. Microorganisms are inoculated and grown under batch regime for a certain amount of time, then nutrients are added to the fermentor in increments throughout the remaining duration of fermentation to feed microorganisms. Continuous fermentation is a method where fresh medium (production medium, fresh feedstock or simply feedstock) is continuously added to the fermentor, while used medium (feedstock) and biomass are harvested at the same time. Consumed nutrients are replaced, and toxic metabolites are removed from the culture. When addition and removal are at the same rate (i.e., the dilution rate), the culture volume and biomass concentration at steady state stay constant. Typically, the inoculum of the filamentous fungi is prepared before the continuous fermentation is performed. Continuous fermentation typically begins with a short batch fermentation using the inoculum without harvesting the biomass. Mycoprotein produced in a batch, fed-batch, or continuous fermentation have typically a crude protein content that is below 56% compared to many of the widely used plant protein concentrates and isolates such as those derived from soy, wheat, or pea. This limits some uses of the mycoprotein where a higher protein content is required, such as the use in the extrusion process to create a structure to meat analogues, or the use as an aquafeed ingredient for carnivorous fish that require a high protein content in their diet. In addition, additional components must be used to bring fat into the final products, such as, polyunsaturated omega-6 fatty acids in case of aquafeed. This in turn increases manufacturing costs. SUMMARY This Summary is provided to introduce a selection of concepts in a simplified form that are further described below in the Detailed Description. This Summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used to limit the scope of the claimed subject matter. It is an object of the present disclosure to provide a technical solution that provides fungal biomasses with increased protein content. It is further an object of the present disclosure to provide a technical solution that provides fungal biomasses with increased polyunsaturated fatty acid content. The objectives above are achieved by the features of the independent claims in the appended claims. Further embodiments and examples are apparent from the dependent claims, and the detailed description. The present disclosure provides novel fungal biomasses comprising one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae; and one or more fatty acids, wherein the fungal biomass has a crude protein content of at least 56 %, based on the total dry weight of the fungal biomass, and wherein the one or more fatty acids comprises one or more polyunsaturated fatty acids, wherein the content of the one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids. The present disclosure also provides methods for the continuous preparation of a fungal biomass, comprising: i) providing one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae; ii) combining a feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, with the one or more filamentous fungi; iii) cultivating the combined one or more filamentous fungi and the feedstock under aerobic conditions to form the fungal biomass; and iv) collecting formed fungal biomass from the feedstock, wherein the dilution rate is 0.3 h-1or less. The present disclosure also provides edible compositions comprising fungal biomass as disclosed herein. The present disclosure also provides uses of a fungal biomass as disclosed herein or an edible composition as disclosed herein as or in foodstuff. DETAILED DESCRIPTION The invention is based on the realization that fungal biomasses comprising filamentous fungi of the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae and one or more fatty acids may be prepared by methods disclosed in this disclosure, wherein these fungal biomasses have a crude protein content of at least 56 %, based on the total dry weight of the fungal biomass, and wherein the one or more fatty acids comprises one or more polyunsaturated fatty acids, wherein the content of the one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids. “Optional” or “optionally” denotes that the subsequently described event or circumstance may but need not occur, and that the description includes instances where the event or circumstance occurs and instances in which it does not. “Comprises” or “comprising” denotes that the subsequently described feature(s) or act(s) may but need not include other feature(s) or act(s). It will further be understood that reference to ‘an’ item refers to one or more of those items. The terms “fungal biomass” as used herein and hereafter refers to the mass of one or more fungal components, such as, but not limited to, proteins, amino acids, fatty acids such as polyunsaturated fatty acid, β-glucan, and chitin. It is to be understood that “fungal biomass” may be single-cell protein (SCP) or two or more fungal components, wherein at least two of the two or more fungal components are protein and at least one polyunsaturated fatty acid, both originating from the fungus / fungi. The terms “single-cell protein” as used herein and hereafter refers to edible unicellular fungus / fungi that comprise protein derived from said fungus / fungi. SCP may or may not be crude or refined unicellular fungus / fungi that may be dead or alive. A fungal biomass comprising one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae is therefore disclosed. The fungal biomass may comprise one or more fatty acids, wherein the fungal biomass has a crude protein content of at least 56 %, based on the total dry weight of the fungal biomass, and wherein the one or more fatty acids comprises one or more polyunsaturated fatty acids, wherein the content of the one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids. The one or more fatty acids, and the one or more polyunsaturated fatty acids, may originate from the one or more filamentous fungi. The protein and the fatty acids, including the one or more polyunsaturated fatty acid(s), may thus originate from the fungus / fungi. In other words, the protein, the one or more fatty acids, and / or the polyunsaturated fatty acid(s) in the fungal biomass may thus originate from the fungus / fungi. Therefore, the fungal biomass may comprise one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae, wherein the fungal biomass comprises one or more fatty acids, wherein the fungal biomass has a crude protein content of at least 56 %, based on the total dry weight of the fungal biomass, and wherein the one or more fatty acids comprises one or more polyunsaturated fatty acids, wherein the content of the one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids. The protein and the one or more fatty acids, and / or the polyunsaturated fatty acid(s), may originate from the fungus / fungi. In other words, the protein, the one or more fatty acids, and / or the one or more polyunsaturated fatty acids may be produced (or may have been produced) by the one or more filamentous fungi. The terms “filamentous fungi” as used herein and hereafter refers to fungi that grow as tubular, elongated, and thread-like (filamentous) structures called hyphae. The terms “fungal family Trichocomaceae” as used herein and hereafter refers to a family of fungi in the order Eurotiales. Examples of fungal genera of the fungal family Trichocomaceae include, but is not limited to, Paecilomyces, Gliocladium, Trichoderma, Byssochlamys, Spicaria, Aspergillus, Penicillium, Rasamsonia, Talaromyces, and Thermoascus. Examples of fungal species of the fungal family Trichocomaceae include, but is not limited to, Paecilomyces variotii, Paecilomyces puntonii, Gliocladium virens, Trichoderma viride, Byssochlamys nivea, Spicaria divaricata, Aspergillus niger, and Aspergillus oryzae. The taxonomy of the order Eurotiales may however be complex and may be or may have been subject to change. According to certain sources, the family Trichocomaceae has been divided into at least three families, i.e. the families Trichocomaceae, Thermoascaceae, and Aspergillaceae. In the context of this specification, the term “fungal family Trichocomaceae” may, at least in some embodiments, thus be understood as referring to or including the fungal families Trichocomaceae, Thermoascaceae, and / or Aspergillaceae. Therefore, examples of fungal species of the fungal family Trichocomaceae may include, but are not limited to, species of the genus Byssochlamys and Rasamsonia, such as Byssochlamys nivea. Therefore, examples of fungal species of the fungal family Thermoascaceae may include, but are not limited to, species of the genus Paecilomyces, such as Paecilomyces variotii and Paecilomyces puntonii. Therefore, examples of fungal species of the fungal family Aspergillaceae may include, but are not limited to, species of the genus Aspergillus, such as Aspergillus niger and Aspergillus oryzae. Therefore, the fungal biomass may comprise one or more filamentous fungi each independently selected from the fungal family Trichocomaceae. Therefore, the fungal biomass may comprise one or more filamentous fungi each independently selected from the fungal family Thermoascaceae. Therefore, the fungal biomass may comprise one or more filamentous fungi each independently selected from the fungal family Aspergillaceae. The terms “fatty acid” and “fatty acids” as used herein and hereafter refers to organic compounds being carboxylic acids comprising a carboxyl group and an aliphatic chain, which may be saturated or unsaturated. Carboxylic acids comprising an unsaturated aliphatic chain may comprise one or more double bonds. Polyunsaturated fatty acids (PUFAs) refer to fatty acids comprising more than one double bond. It is to be understood that fatty acids (e.g., PUFAs) as disclosed in the present disclosure may also refer to the fatty acid part(s) of triglycerides and phospholipids, i.e., the fatty acids of triglycerides from glycerol and fatty acids, and the fatty acids of phospholipids from phosphate and fatty acids. Examples of saturated fatty acids include, but are not limited to, palmitic acid (C16:0, hexadecenoic acid), and stearic acid (C18:0, octadecanoic acid). Examples of unsaturated fatty acids include, but are not limited to, palmitoleic acid (C16:1 ω7, (9Z)-hexadec-9-enoic acid), oleic acid (C18:1 ω9, (9Z)-octadec-9-enoic acid), and linoleic acid (C18:2 ω6, (9Z,12Z)-octadeca-9,12-dienoic acid). Examples of PUFAs include, but are not limited to, omega-6 fatty acids. Examples of omega-6 fatty acids include, but are not limited to, linoleic acid (C18:2 ω6, (9Z,12Z)-octadeca-9,12-dienoic acid), gamma-linolenic acid (GLA, C18:3 ω6, (6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid), and eicosadienoic acid (C20:2 ω6, (11Z,14Z)-icosa-11,14-dienoic acid). Other examples of fatty acids than the above-mentioned fatty acids include, but are not limited to, omega-7 fatty acids, and omega-9 fatty acids. Examples of omega-7 fatty acids include, but are not limited to, palmitoleic acid (C16:1 ω7, (9Z)-hexadec-9- enoic acid), and vaccenic acid (C16:1 ω7, (11E)-octadec-11-enoic acid). Examples of omega-9 fatty acids include, but are not limited to, oleic acid (18:1 ω9, (9Z)-octadec-9-enoic acid), and erucic acid (22:1 ω9, (13Z)-docos-13-enoic acid). The terms “crude protein content” as used herein and hereafter refers to the protein content of fungal biomass, composition, single-cell protein, or to which it may refer to and corresponds to the amount of nitrogen of said fungal biomass, composition, or single-cell protein multiplied by 6.25 (percentage of protein = 6.25 * N%) (dry weight basis of the fungal biomass, composition, or single-cell protein). Typically, crude protein content refers to the protein content of the fungal biomass based on the total dry weight of the fungal biomass. The crude protein content may be determined by methods known by the person skilled in the art, for example methods including, but not limited to, the Kjeldahl method and the Dumas method. It is to be understood that the protein of the crude protein content of the fungal biomass refers to protein that originates from the one or more filamentous fungi. E.g., a crude protein content of 65 % corresponds to ca. 500 – 550 g of amino acids per 1 kg of fungal biomass, based on the total dry weight of the fungal biomass. Furthermore, it is to be understood that an edible composition comprising fungal biomass as disclosed herein and hereafter may or may not contain further protein that may originate from other protein sources than the filamentous fungus as disclosed herein and hereafter. The terms “dry weight” as used herein and hereafter refers to the mass of dried fungal biomass, composition, or single-cell protein, i.e., the mass of fungal biomass, composition, or single- cell protein excluding water. The term “wt%” as used herein and hereafter refers to percentage by mass, i.e., the mass fraction (wi) of the mass (mi) to the total mass (mtot) times a denominator of 100, i.e., the formula wt% = wi* 100 = (mi / mtot) * 100, wherein wi= mass fraction, mi= mass of substance, protein, or compound to which wt% refers to, and mtot= the total mass of e.g., fungal biomass, feedstock, aqueous culture medium, or composition. The Paecilomyces variotii strain KCL-24 is deposited with the recognised depositary institution VTT Culture Collection (VTTCC, Finland) with the accession number VTT D-211703. The strain KCL-24 of fungus Paecilomyces variotii is deposited 27 August 2021 under the Budapest Treaty by eniferBio Oy in the VTT Culture Collection (VTTCC, VTT Technical Research Centre of Finland Ltd, P.O. Box 1000, FI-02044 VTT, Finland) with an accession number VTT D-211703 (identification reference given by the depositor: KCL-24, PEKILO). The strain was received by the Depositary Authority on 27 August 2021. The culture was confirmed viable on 30 August 2021. The strain is characterized by the following morphological, culture and biochemical properties. Morphological properties: a filamentous fungus, aerobic. Culture properties: Forms white flat and powdery colonies on potato dextrose agar having from white to yellow colour. Filamentous growth on submerged cultivation. Optimal cultivation temperature at 37 °C. Physiological properties: A filamentous fungus with a high specific growth rate (~0.5 h-1), ability to utilize many carbon sources, in particular, C5 sugars, acetate, formate, and glycerol. High tolerance toward thermal biomass degradation products (furfural, formic acid, levulinic acid, 5-HMF and phenolics). Good filtration characteristics, high protein and beta-glucan content. It grows at a pH 3-7.5 at temperature 20-45 °C. Uses ammonia, urea and amino acids as a sole nitrogen source. Pathogenicity: the strain is not pathogenic. On the basis of the genome sequencing the strain is attributed to the genus Paecilomyces, variotii species. The method of cultivating the fungal strain Paecilomyces variotii KCL-24 may be carried out as described in the section EXAMPLES 1-4 of this disclosure. The term “fiber” as used herein and hereafter refers to the primary compounds of cell walls in, e.g., fungi. Examples of fibers include, but are not limited to, chitin, and glucans. The term “β-glucan” as used herein and hereafter refers to a group of β-D-glucose polysaccharides occurring in the cell walls of, e.g., fungi. Typically, β-glucans form a linear backbone with 1–3 β-glycosidic bonds but vary with respect to molecular mass, solubility, viscosity, branching structure, and gelation properties. Common forms of β-glucans are those comprising D- glucose units with β-1,3 links. Fungal β-glucans may contain for example 1-6 side branches. The term “ash” as used herein and hereafter refers to inorganics produced by combustion of biomass. Examples of inorganics comprised in biomass include, but are not limited to, calcium, iron, sodium, potassium, magnesium, phosphorous, and minerals thereof. The term “fat” as used herein and hereafter refers to compounds that are typically lipophilic (i.e., non-water soluble) compounds. Fat may comprise one or more different lipophilic compound. Examples of compounds that fat may comprise include, but are not limited to, esters of fatty acids, fatty acids and salts thereof; mono-, di-, triglycerids, phospholipids, and / or cholesterol, or any combinations thereof. The terms “water content” in combination with “wt%” as used herein and hereafter refers to water content percentage of the biomass or what it refers to, based on the total weight of the fungal biomass. The term “feedstock” as used herein and hereafter refers to processed or unprocessed feedstock and fresh feedstock that one or more filamentous fungi may be cultivated in, i.e., the feedstock may be used as such as an aqueous culture medium for cultivating one or more filamentous fungi as disclosed herein and hereafter. The feedstock typically comprises water and one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, and optionally one or more organic compounds and / or one or more inorganic compounds. The feedstock may be processed by removing, at least partially, insoluble solids from the feedstock, and / or diluting or concentrating the feedstock before, during, and / or after, or any combination thereof, cultivating the one or more filamentous fungi as disclosed herein and hereafter in the feedstock. It is to be understood that feedstock may also refer to used feedstock (e.g., used aqueous culture medium), wherein one or more filamentous fungi as disclosed herein and hereafter has been cultivated and therefore, the amount of one or more dissolved carbon sources of the used feedstock may be lower than in feedstock, wherein one or more filamentous fungi as disclosed herein and hereafter has not been cultivated (i.e., fresh feedstock), i.e., the composition of the feedstock may vary during a method as disclosed herein and hereafter. For example, before the one or more filamentous fungi is cultivated in the feedstock, the feedstock may comprise a higher content of one or more dissolved carbon sources than during and / or after said cultivation of the one or more filamentous fungi. It is to be understood that the feedstock may be an aqueous culture medium. The feedstock as such may comprise furfural, and if the feedstock does not comprise furfural, furfural may be added to the feedstock. During continuous cultivation of fungal biomass, feedstock, i.e., fresh feedstock, is typically added to the fermentor at the same rate as formed biomass is removed from the fermentor. “Fresh feedstock” refers to feedstock comprising one or more carbon sources, wherein one or more filamentous fungi has not been cultivated in, or to feedstock comprising higher content of one or more carbon sources than feedstock comprising one or more carbon sources, wherein one or more filamentous fungi has been cultivated in. It is to be understood that during a method for the continuous preparation of a fungal biomass as disclosed in the present disclosure the composition of the feedstock (fresh feedstock) may change, e.g., the content of furfural in the feedstock may be changed such that the content of furfural is higher or lower in the feedstock (fresh feedstock) during the method. Additionally, or alternatively, different feedstocks may be used in the methods for the continuous preparation of fungal biomass as disclosed in the present disclosure, as long as at least one of the feedstocks used during the methods comprises furfural. The terms “aqueous culture medium” as used herein and hereafter refers to growth medium comprising water and one or more carbon sources (such as furfural) and optionally one or more organic compounds and / or one or more inorganic compounds, wherein the aqueous culture medium allows and / or promotes growth and / or cell proliferation of microorganisms, such as fungus as disclosed herein and hereafter. It is to be understood that the aqueous culture medium may be a feedstock as disclosed herein and hereafter. Aqueous culture medium may comprise furfural. Examples of organic compounds include, but are not limited to, aldehydes such as furfural, carbohydrates, carbohydrate derivatives, sugars, polyols, carboxylic acids, amino acids, alcohols, esters of carboxylic acids, antifoaming agents such as Struktol J673A (alkoxylated fatty acid esters on vegetable base from Schill + Seilacher GmbH), and any combinations thereof. Examples of inorganic compounds include, but are not limited to, nitrogen containing compounds such as NH4OH, (NH4)2SO4, CH₄N₂O, (NH4)2HPO4; and phosphorous containing compounds such as H3PO4, and phosphates; KCl, MgSO4, Fe2(SO4)3, ZnSO4, CuSO4, MnSO4, HCl, Vogel's trace elements, and any hydrates and combinations thereof. Vogels’s trace elements typically comprises citric acid (e.g., 5 wt%), ZnSO4(e.g., 5 wt%), Fe(NH4)2(SO4)2(e.g., 1 wt%), CuSO4(e.g., 0.25 wt%), MnSO4(e.g., 0.05 wt%), H3BO3(e.g., 0.05 wt%, and Na2MoO4(e.g., 0.05 wt%) dissolved in water. A person skilled in the art is aware that the aqueous culture medium (and the feedstock) may further comprise trace elements (inorganic trace substances) that may be beneficial for the growth and / or cell proliferation of the one or more filamentous fungi. It is to be understood that the aqueous culture medium may be the feedstock as defined herein and hereafter. Examples of feedstocks and aqueous culture media include, but are not limited to, stillage, thin stillage, vinasse, molasses, spent sulphite liquor, prehydrolysis liquor, food industry processing waste, and other biorefinery by-products, or any mixture or combination thereof. Said feedstock may be or may not be a clarified feedstock, i.e., a feedstock, which has been treated in order to remove, at least partially, insoluble or suspended solids thereof. “Thin stillage” as used herein and hereafter refers to stillage (from ethanol production using, e.g., corn or wheat), which solids have been partially removed by, e.g., centrifugation and therefore, it is to be understood that thin stillage may comprise insoluble solids. “Vinasse” as used herein and hereafter refers to a by- product of the sugar and ethanol industry. Vinasse is obtained as a by-product of the distillation step subsequent to fermentation of carbohydrates obtained from different sources of saccharides materials (e.g., sugarcane and beet), starchy materials (e.g., maize, wheat, rice, cassava, and oat), and lignocellulosic materials (e.g., sugarcane bagasse, straw, and wood, among others). “Molasses” as used herein and hereafter refers to a product resulting from refining sugarcane or sugar beets into sugar. Molasses may also refer to a condensate from a biorefining process of lignocellulosic biomass. Typically, molasses comprises one or more sugars, e.g., saccharose, glucose, and / or fructose. For example, molasses may comprise 35 – 65 wt% sugars, typically 44 wt% sugars, e.g., 44 wt% saccharose. “Spent sulphite liquor” as used herein and hereafter refers to spent cooking liquor from sulfite pulping and is also called brown liquor, red liquor, thick liquor, and sulfite liquor. “Prehydrolysis liquor” as used herein and hereafter refers to a liquor from the pre-hydrolysis stage in the dissolving pulp production process, that may be rich in one or more dissolved carbon sources derived from hemicellulose, such as sugars and carboxylic acids. Examples of food industry processing waste include, but are not limited to, brewery wastewater from beer brewing, pot ale and spent lees from the manufacture of whisky, potato processing waste, dairy subproducts such as whey permeate and delactosed permeate. Examples of other biorefinery by-products include, but are not limited to, corn steep liquor, soy molasses, and palm mill oil effluent (POME). The terms “solids that are insoluble” and “insoluble solids” as used herein and hereafter refers to any nondissolved organic- and inorganic compounds, carbon sources, and chemical elements of feedstocks and / or aqueous culture media, i.e., organic- and inorganic compounds, carbon sources, and chemical elements that are not dissolved. Examples of solids that are insoluble in a feedstock include, but are not limited to, insoluble solids of biorefinery by-products, such as, but not limited to, cellulose; insoluble biomaterial, insoluble solids and suspended solids originating from fermentation process, molasses, thin stillage, vinasse, spent sulphite liquor, prehydrolysis liquor, and food industry processing waste, such as, but not limited to, CaSO4. Solids that are insoluble in a feedstock may also refer to solids, such as carbon sources, that are soluble in a feedstock to a certain concentration, but the feedstock is saturated by said solid and therefore, at least a part of the solid is in the form of a precipitate or suspension. In addition, it is to be understood that “solids that are insoluble” and “insoluble solids” exclude fungal biomass as disclosed herein and hereafter. The terms “fungal biomass is essentially free from solids that are insoluble” as used herein and hereafter refers to biomasses that contains no, very low, or low amounts of solids originating from solids that are insoluble in a feedstock and / or an aqueous culture medium used to cultivate the filamentous fungi as disclosed herein and hereafter to form the fungal biomass, wherein the solids may be any organic- and / or inorganic compounds and / or chemical elements that are insoluble in the feedstock. In this context it is to be understood that the fungal biomasses may comprise solids that are insoluble in a feedstock, however, these insoluble solids may have formed during the proliferation of the filamentous fungus, i.e., during a method for the continuous preparation of a fungal biomass as disclosed in the present disclosure. “Fungal biomass is essentially free from solids that are insoluble in a feedstock” may be fungal biomass containing <1.0 wt%, preferably <0.5 wt%, more preferably 0 wt%, of solids that are insoluble in a feedstock comprising one or more dissolved carbon sources, based on the total weight of the feedstock, and said solids that are insoluble may have a size of >1.0 µm, preferably >0.5 µm, more preferably >0.2 µm. It is to be understood that a fungal biomass having both a smaller content and a smaller size of solids that are insoluble in a feedstock is desirable. “Essentially free” in this context may also mean that solids that may be insoluble in a feedstock may have been removed, at least partially, from the feedstock, which may be used in a method for the continuous preparation of a fungal biomass as disclosed herein and hereafter to form the fungal biomass, and therefore, the fungal biomass (formed by cultivating the combined one or more filamentous fungi and the feedstock) may not contain the removed solids. Preferably, solids that are insoluble in a feedstock and with a size of at least 1.0 µm, preferably at least 0.5 µm, more preferably at least 0.2 µm, have been removed, at least partially, from the feedstock, preferably >50 %, >70 %, >80 %, >90 %, >95 %, >97 %, or >99 %, of the solids that are insoluble in a feedstock have been removed from the feedstock. More preferably, “fungal biomass is essentially free from solids that are insoluble in a feedstock” refers to biomass that is free of solids that are insoluble in a feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural. The term “carbon sources” as used herein and hereafter refers to molecules used by an organism as the source of carbon for building its biomass. Examples of carbon sources include, but are not limited to, carbohydrates, carbohydrate derivatives, aldehydes, polyols, carboxylic acids, esters of carboxylic acids, nucleotides, and alcohols. The term “carbohydrates” as used herein and hereafter refers to compounds comprising oxygen, hydrogen, and at least one carbon. Examples of carbohydrates include, but are not limited to, sugars, oligosaccharides, and polysaccharides such as glucose, mannose, xylose (D-xylose), arabinose, galactose, fructose, sucrose, maltose, isomaltulose, trehalose, lactose, maltotriose, maltodextrins, xylooligosaccharides (XOS), raffinose, stachyose, and fructo-oligosaccharides. The terms “carbohydrate derivatives” as used herein and hereafter refers to carbohydrates that have been modified with one or more substituents. Carbohydrate derivatives may also be carbohydrates or carbohydrate analogues that further comprise one or more heteroatom each independently selected from the group consisting of N, S, and Se. Examples of carbohydrate derivatives include, but are not limited to, glycosides, glycosylamines, N- acetylglucosamine, sugar phosphates, and esters of carbohydrates such as carbohydrate acetates. It is to be understood that the carbohydrate derivative may or may not function as a precursor material or an intermediate in the biosynthesis of, or conversion to, a carbohydrate. The term “aldehydes” as used herein and hereafter refers to organic compounds comprising at least one aldehyde group. Examples of aldehydes include, but are not limited to, furfural (furan-2-carbaldehyde). The term “polyols” as used herein and hereafter refers to compounds comprising at least two hydroxyl groups. Examples of polyols include, but are not limited to, glycerol, mannitol, and sorbitol. The terms “carboxylic acids” as used herein and hereafter refers to compounds comprising at least one a carboxyl group. Examples of carboxylic acids include, but are not limited to, formic acid, acetic acid, lactic acid, propionic acid, sugar acids, and amino acids. The term “esters of carboxylic acids” as used herein and hereafter refers to compounds derived from carboxylic acids, wherein at least one OH-group has been replaced by an alkoxy group. Examples of alkoxy groups include, but are not limited to, methoxy, ethoxy, and alkoxy groups derived from carbohydrates and carbohydrate derivatives. The term “alcohols” as used herein and hereafter refers to compounds comprising a hydroxyl group. Examples of alcohols include, but are not limited to, methanol, ethanol, and ethylene glycol. The term “edible” as used herein and hereafter refers to any substance, fungal biomass, and composition that is considered safe for animals and / or humans to eat such as, but not limited to, foodstuff. The term “foodstuff” as used herein and hereafter refers to food for consumption of humans and / or animals such as, but not limited to, fish, shrimps, crayfish, prawns, and domestic animals such as pig, sheep, cattle, and chicken. It is to be understood that foodstuff includes food for consumption of humans, compound feed, fodder, and animal feed, such as, but not limited to, fish feed, and aquafeed. The term “continuous preparation” as used herein and hereafter refers to continuous cultivation (continuous fermentation) of one or more filamentous fungi to form fungal biomass. Typically, during continuous preparation fresh feedstock is continuously added to a bioreactor containing one or more filamentous fungi and feedstock, while fungal biomass and often also culture liquid comprising left over nutrients (e.g., one or more dissolved carbon sources)and / or metabolic end products are continuously removed at the same rate as the addition of the fresh feedstock to keep the culture volume constant. By changing the rate with which fresh feedstock is added to the bioreactor (i.e., the dilution rate) the specific growth rate of the fungus / fungi may be controlled. Examples of bioreactors include, but are not limited to, stirred tank, airlift, and any system suitable for cultivation of one or more filamentous fungi to form fungal biomass. The term “removing, at least partially, insoluble solids from the feedstock” and “removed” in the same context as used herein and hereafter refers to any method of removing insoluble solids, wherein the amount of insoluble solids decreases. Examples of removing include, but are not limited to, filtering, clarification, decantating, settling, centrifugating, and screening. The term “diluting” as used herein and hereafter refers to any method of decreasing the concentration of one or more dissolved carbon sources of the feedstock. Examples of diluting include, but are not limited to, adding water, solvent, one or more organic compounds, provided that the one or more organic compounds are selected from other organic compounds than said one or more dissolved carbon sources; and / or inorganic compounds, or combinations thereof, and another feedstock to the feedstock. The term “concentrating” as used herein and hereafter refers to any method of increasing the concentration of the one or more dissolved carbon sources of the feedstock and / or aqueous culture medium. Examples of concentrating include, but are not limited to, addition of carbon sources to the feedstock and / or aqueous culture medium, removing such as evaporating water and / or solvent from the feedstock, and dialysis of the feedstock and / or aqueous culture medium. The term “cultivating” as used herein and hereafter refers to the process of growth and / or cell proliferation of microorganisms, such as fungus. The terms “aerobic conditions” as used herein and hereafter refers to conditions that include oxygen. The terms “collecting formed fungal biomass from the feedstock” as used herein and hereafter refers to any method by which formed fungal biomass may be collected from feedstock. Examples of collecting formed fungal biomass from feedstock include, but are not limited to, filtering, decantating, settling, centrifugating, and screening. The terms “dilution rate” used herein and hereafter refers to the rate of feedstock exchange in methods of continuous preparation of fungal biomass as disclosed herein and hereafter. During continuous preparation, when formed fungal biomass is continuously collected from the feedstock, fresh feedstock is continuously added at the same rate as collecting formed fungal biomass to keep the culture volume constant. By changing the rate fresh feedstock is added to the bioreactor (i.e., the dilution rate (D)), the specific growth rate of the fungus / fungi may be controlled. The dilution rate (D) may be at steady state equal to the specific growth rate (μ) of the one or more filamentous fungi. Steady state refers to the situation, wherein growth occurs at a constant specific growth rate and all culture parameters may remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.). The dilution rate (D) is defined as flow of feedstock per unit of time (F) over volume (V) of filamentous fungi culture (the volume (V) of filamentous fungi culture refers to the volume of feedstock and one or more filamentous fungi in, e.g., the bioreactor): D = F / V . The term “drying” as used herein and hereafter refers to any method of removing water (dewatering) or another solvent. Examples of drying include, but are not limited to, drying using hot air (with a temperature selected from, for example, 40 – 80 °C, 50 – 70 °C, or 50 °C), filtering, freeze drying, indirect or contact drying (such as heating through a hot wall) such as drum drying and vacuum drying; natural air drying. Examples of drying using hot air include, but is not limited to, the use of conveyor dryer, fluid bed dryer, flash dryer, and ring dryer. The terms “cell density” as used herein and herafter refers to the number or mass of cells per unit volume. Cell density may be denoted as viable cell density which is the number or mass of living cells per unit volume. The terms “food ingredient” as used herein and herafter refers to any substance and composition that is considered safe for animals and / or humans to eat. Food ingredients may be added to, e.g., foodstuff (food for consumption of humans, animal feed, aquafeed, fodder, compound feed), and fungal biomasses and edible compositions as disclosed herein and herafter to achieve a desired effect. Examples of food ingredients include, but are not limited to, fishmeal, fish oil, fish feed, soy protein concentrate, soy, soybeans, wheat protein, pea protein, soy protein isolate, wheat protein isolate, pea protein isolate, protein sources, food additives such as acidulants, acidity regulators, anticaking agents, antifoaming and foaming agents, antioxidants, bulking agents, food coloring, fortifying agents, color retention agents, emulsifiers, flavors, flavor enhancers, flour treatment agents, glazing agents, humectants, tracer gas, preservatives, stabilizers, sweeteners, and thickeners; corn, grain sorghum, oats, rye, barley; flours such as wheat, rye, farina, and meal flours; dairy products such as milk, yoghurt, curdled milk (soured milk), and cheeses such as cottage cheese. It is to be understood that a fungal biomass as disclosed herein and hereafter may be a food ingredient in foodstuff. The term “fish feed” as used herein and hereafter refers to foodstuff for fish. Conventional fish feed may contain fishmeal, fish oil, and / or SPC. The term “fishmeal” as used herein and hereafter refers to a food ingredient that fish feed may comprise, wherein fishmeal is mostly made from fish or parts thereof. Typically, fishmeal is food used to feed fish. Fishmeal may be made by cooking, pressing, drying, and / or grinding of fish or fish waste into a solid, wherein at least partially the water and some or all of the oil is removed. The term “aquafeed” as used herein and hereafter refers to foodstuff for aquatic organisms such as, but not limited to, fish, shrimps, crayfish, and prawns. In one aspect is disclosed a novel fungal biomass comprising one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae; and one or more fatty acids, wherein the fungal biomass has a crude protein content of at least 56 %, based on the total dry weight of the fungal biomass, and wherein the one or more fatty acids comprises one or more polyunsaturated fatty acids, wherein the content of the one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids. A crude protein content of at least 56 % is considered as a high protein content. A fungal biomass having a crude protein content of at least 56 % is beneficial, since the fungal biomass may be used as an (cost-effective) alternative to soy protein or SPC of conventional fish feed or aquafeed. Additionally, a content of the one or more polyunsaturated fatty acids comprised in the fungal biomass being at least 56 wt%, based on the total weight of the one or more fatty acids, is advantageous since such a high content of one or more polyunsaturated fatty acids of the fungal biomass is beneficial in foodstuff, which is, or comprises, the fungal biomass. Foodstuff comprising a high content (at least 56 wt%) of polyunsaturated fatty acids may be considered healthier than foodstuff comprising, e.g., a lower (less than 56 wt%) content of polyunsaturated fatty acids and / or a high content of monounsaturated fatty acids and / or saturated fatty acids. It is to be understood that the terms “the content the one of the one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids” as disclosed in the present disclosure refers to the total content of the one or more polyunsaturated fatty acids being at least 56 wt%, based on the total weight of the one or more fatty acids. Furthermore, it is to be understood that to the content of polyunsaturated fatty acids (PUFAs) and to the total weight of fatty acids include PUFAs and fatty acids that would be obtained when triglycerides and phospholipids of the biomass are hydrolysed. Therefore, the fungal biomasses as disclosed in the present disclosure may comprise a first polyunsaturated fatty acid (e.g., linoleic acid), wherein the content of the first polyunsaturated fatty acid is at least 56 wt%, or the fungal biomasses as disclosed in the present disclosure may comprise a plurality of polyunsaturated fatty acids (e.g., at least linoleic acid and eicosadienoic acid (C20:2 ω6, (11Z,14Z)-icosa-11,14- dienoic acid), wherein the total content of the plurality of polyunsaturated fatty acids is at least 56 wt%, or the fungal biomasses as disclosed in the present disclosure may comprise a first polyunsaturated fatty acid (e.g., linoleic acid) and one or more polyunsaturated fatty acids being different to the first polyunsaturated fatty acid (e.g., at least gamma-linolenic acid), wherein the content of the first polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids. Additionally, or alternatively, the fungal biomass as disclosed herein and hereafter may be used to, at least partially, replace soy protein or SPC of conventional fish feed and aquafeed, and therefore, the fungal biomass may be an ecological alternative to soy protein and SPC. Due to the high crude protein content and the high content of the one or more polyunsaturated fatty acids of the fungal biomass as disclosed herein and hereafter, foodstuff, preferably aquafeed or fish feed, comprising fungal biomass as disclosed herein and hereafter has lower production cost than foodstuff comprising conventional biomasses. Additionally, or alternatively, the fungal biomass has a crude protein content of 56-99 %, based on the total dry weight of the fungal biomass. Additionally, or alternatively, the fungal biomass has a crude protein content of 56-94 %, 56-91 %, 56-87 %, 56-83 %, 56-79 %, 56-75 %, 56-73 %, 58-83 %, 58-83 %, 58-79 %, 58- 74 %, 60-70 %, 60-74 %, 60-77 %, 60-81 %, 60-85 %, 60-89 %, 60-93 %, 60-95 %, 60-99 %, 61-70 %, 61-74 %, 61-77 %, 61-81 %, 61-85 %, 61-89 %, 61-93 %, 61-95 %, or 61-99 %. These fungal biomasses are beneficial as or in foodstuff, preferably aquafeed or fish feed. Additionally, or alternatively, the fungal biomass has a crude protein content of 63-99 %, 63-95 %, 63-91 %, 63-87 %, 63-83 %, 63-79 %, 63-75 %, 63-74 %, 63-70 %, 65-99 %, 65-95 %, 65-91 %, 65- 87 %, 65-83 %, 65-79 %, 65-75 %, or 65-74 %. These fungal biomasses are beneficial as or in foodstuff, preferably aquafeed or fish feed. Additionally, or alternatively, the fungal biomass has a crude protein content of 61-74 % based on the total dry weight of the fungal biomass. These fungal biomasses are beneficial as or in foodstuff, preferably aquafeed or fish feed. Additionally, or alternatively, the one or more filamentous fungi is each independently selected from the fungal genera Paecilomyces, Aspergillus, Penicillium, Rasamsonia, Talaromyces, and Thermoascus. Fungal biomasses of these fungal genera are suitable as or in foodstuff. Additionally, or alternatively, the one or more filamentous fungi is each independently selected from the fungal species Paecilomyces variotii, Paecilomyces puntonii, Gliocladium virens, Trichoderma viride, Byssochlamys nivea, Spicaria divaricata, Aspergillus niger, and Aspergillus oryzae. Fungal biomasses comprising fungal species Paecilomyces variotii, Paecilomyces puntonii, Aspergillus niger, and / or Aspergillus oryzae, or any combination thereof, are especially suitable as or in foodstuff. Additionally, or alternatively, the one or more filamentous fungi comprises or is Paecilomyces variotii strain KCL-24, preferably is Paecilomyces variotii strain KCL-24. It has surprisingly been found that fungal biomasses comprising or consisting of Paecilomyces variotii strain KCL-24 may have a high crude protein content, i.e., a crude protein content of at least 56 % based on the total dry weight of the fungal biomass. Additionally, it has surprisingly been found that fungal biomasses comprising or consisting of Paecilomyces variotii strain KCL-24 may have a high content of one or more polyunsaturated fatty acids, i.e., the one or more polyunsaturated fatty acids content is at least 56 wt%, based on the total weight of the one or more fatty acids. These fungal biomasses are beneficial, since Paecilomyces variotii strain KCL-24 has a suitable amino acid composition for foodstuff, a good protein digestibility in animals, and lack mycotoxins. Alternatively, or additionally, the fungal biomass comprising Paecilomyces variotii strain KCL-24 as disclosed herein and hereafter has a crude protein content of 56-94 %, 56-91 %, 56- 87 %, 56-83 %, 56-79 %, 56-75 %, 56-73 %, 58-83 %, 58-83 %, 58-79 %, 58-74 %, at least 60 %, 60-70 %, 60-74 %, 60-77 %, 60-81 %, 60- 85 %, 60-89 %, 60-93 %, 60-95 %, 60-99 %, at least 61 %, 61-70 %, 61-74 %, 61-77 %, 61-81 %, 61-85 %, 61-89 %, 61-93 %, 61-95 %, or 61-99 %, based on the total dry weight of the fungal biomass. These fungal biomasses are beneficial as or in foodstuff, preferably aquafeed or fish feed. Additionally, or alternatively, the fungal biomass comprising Paecilomyces variotii strain KCL-24 has a crude protein content of 63-99 %, 63-95 %, 63-91 %, 63-87 %, 63-83 %, 63-79 %, 63-75 %, 63-74 %, 63-70 %, 65-99 %, 65-95 %, 65-91 %, 65- 87 %, 65-83 %, 65-79 %, 65-75 %, 65-74 %, or 65-70 %. These fungal biomasses are beneficial as or in foodstuff, preferably aquafeed or fish feed. Additionally, or alternatively, the fungal biomass comprises Paecilomyces variotii strain KCL-24, wherein the fungal biomass has a crude protein content of 56-74 %, 61-74 %, or 65-74 %, based on the total dry weight of the fungal biomass. These fungal biomasses are beneficial as or in foodstuff, preferably aquafeed or fish feed. Additionally, or alternatively, the fungal biomass consists of Paecilomyces variotii strain KCL-24, wherein the fungal biomass has a crude protein content of 56-74 %, 61-74 %, or 65-74 %, based on the total dry weight of the fungal biomass. These fungal biomasses are beneficial as or in foodstuff, preferably aquafeed or fish feed. Additionally, or alternatively, at least one of the one or more polyunsaturated fatty acids is selected from an omega-6 fatty acid. These fungal biomasses are beneficial since omega-6 fatty acids are healthy in foodstuff. Additionally, or alternatively, the one or more polyunsaturated fatty acids is linoleic acid (C18:2 ω6, (9Z,12Z)- octadeca-9,12-dienoic acid). These fungal biomasses are beneficial since linoleic acid is healthy in foodstuff. Additionally, or alternatively, the one or more polyunsaturated fatty acids is a plurality of polyunsaturated fatty acids, wherein at least one of the plurality of polyunsaturated fatty acids is selected from omega-6 fatty acids. These fungal biomasses are beneficial since omega-6 fatty acids are healthy in foodstuff. Additionally, or alternatively, one of the plurality of polyunsaturated fatty acids is selected from linoleic acid (C18:2 ω6, (9Z,12Z)-octadeca-9,12-dienoic acid), gamma-linolenic acid (GLA, C18:3 ω6, (6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid), and eicosadienoic acid (C20:2 ω6, (11Z,14Z)-icosa-11,14-dienoic acid). Additionally, or alternatively, one of the plurality of polyunsaturated fatty acids is linoleic acid (C18:2 ω6, (9Z,12Z)- octadeca-9,12-dienoic acid). These fungal biomasses are beneficial since omega-6 fatty acids are healthy in foodstuff. Additionally, or alternatively, the content of at least one of the one or more polyunsaturated fatty acids is at least 56 wt%, or it is 56 – 90 wt%, 56 – 85 wt%, 56 – 80 wt%, 56 – 70 wt%, 60 – 90 wt%, 60 – 85 wt%, 60 – 80 wt%, 60 – 75 wt%, 60 – 70 wt%, 65 – 90 wt%, 65 – 85 wt%, 65 – 80 wt%, 65 – 75 wt%, or 65 – 70 wt%, based on the total weight of the one or more fatty acids. These fungal biomasses are advantageous, since with these fungal biomasses there may be no need to add additional components to bring fat into the final products. This in turn decreases manufacturing costs of products comprising the fungal biomass as disclosed in the present disclosure. Additionally, or alternatively, the total content of the one or more polyunsaturated fatty acids is at least 56 wt%, or it is 56 – 90 wt%, 56 – 85 wt%, 56 – 80 wt%, 56 – 70 wt%, 60 – 90 wt%, 60 – 85 wt%, 60 – 80 wt%, 60 – 75 wt%, 60 – 70 wt%, 65 – 90 wt%, 65 – 85 wt%, 65 – 80 wt%, 65 – 75 wt%, or 65 – 70 wt%, based on the total weight of the one or more fatty acids. These fungal biomasses are advantageous, since with these fungal biomasses there may be no need to add additional components to bring fat into the final products. This in turn decreases manufacturing costs of products comprising the fungal biomass as disclosed in the present disclosure. Additionally, or alternatively, one of the one or more polyunsaturated fatty acids is linoleic acid. These fungal biomasses are advantageous, since linoleic acid may be an essential fatty acid for human and animals, and, therefore, foodstuff comprising linoleic acid may be considered healthy. Additionally, or alternatively, the content of linoleic acid is at least 56 wt%, based on the total weight of the one or more fatty acids. Additionally, or alternatively, the one or more polyunsaturated fatty acids is linoleic acid, wherein the content of linoleic acid is at least 56 wt%, based on the total weight of the one or more fatty acids. Additionally, or alternatively, the content of linoleic acid is 56 – 90 wt%, 56 – 85 wt%, 56 – 80 wt%, 56 – 70 wt%, 60 – 90 wt%, 60 – 85 wt%, 60 – 80 wt%, 60 – 75 wt%, 60 – 70 wt%, 65 – 90 wt%, 65 – 85 wt%, 65 – 80 wt%, 65 – 75 wt%, or 65 – 70 wt%, based on the total weight of the one or more fatty acids. Additionally, or alternatively, the content of linoleic acid is at least 69 wt%, or it is about 70 wt% or 69 – 70 wt%, based on the total weight of the one or more fatty acids. These fungal biomasses are advantageous, since foodstuff comprising a high content (i.e., at least 56 wt%) of linoleic acid is considered healthy. In addition, these fungal biomasses are advantageous, since with these fungal biomasses there may be no need to add additional components to bring fat into the final products, such as, in case of aquafeed, polyunsaturated omega-6 fatty acid. This in turn decreases manufacturing costs of products comprising the fungal biomass as disclosed in the present disclosure. Additionally, or alternatively, the fungal biomass consists of Paecilomyces variotii strain KCL-24, the fungal biomass has a crude protein content of 56-74 %, 61-74 %, or 65-74 %, based on the total dry weight of the fungal biomass, and the content of linoleic acid is at least 69 wt%, or it is about 70 wt% or 69 – 70 wt%, based on the total weight of the one or more fatty acids. These fungal biomasses are beneficial as or in foodstuff, preferably aquafeed or fish feed. Alternatively, or additionally, the content of oleic acid is less than 20 wt%, preferably less than 15 wt%, more preferably less than 14 wt%, based on the total weight of fatty acids of the fungal biomass. The terms “oleic acid” refers to (9Z)-octadec-9- enoic acid (oleic acid (C18:1 ω9)). These fungal biomasses are advantageous, since they comprise less of oleic acid, an unhealthy fatty acid. Alternatively, or additionally, the content of palmitic acid is less than 16 wt%, preferably less than 15 wt%, based on the total weight of fatty acids of the fungal biomass. The terms “palmitic acid” refers to hexadecenoic acid (palmitic acid (C16:0)). These fungal biomasses are advantageous, since they comprise less of palmitic acid, an unhealthy fatty acid. Additionally, or alternatively, the fungal biomass comprises 0.5-10 wt% water, a total fiber content of 10-35 wt%, a β-glucan content of 10-25 wt%, 1-10 wt% ash, and 1-10 wt% fat, based on the total weight of the fungal biomass. These fungal biomasses have beneficial fish immunostimulant properties and are beneficial for fish health. Additionally, or alternatively, the fungal biomass comprises 0.5-10 wt% water and 90-99.5 wt% Paecilomyces variotii strain KCL-24 based on the total weight of the fungal biomass, wherein the crude protein content of the fungal biomass is selected from 56-74 %, 61-74 %, and 65-74 %, based on the total dry weight of the fungal biomasses. Additionally, or alternatively, the fungal biomass has a water content of 3-8 wt%, preferably 4-7 wt%, based on the total weight of the fungal biomass. These fungal biomasses have a preferred protein digestibility. In addition, the fungal biomasses have a preferred shelf-life and enable cost-effective transportation due to the low content of water. Furthermore, the water content of the fungal biomasses enables effective use in apparatus for preparation of foodstuff, preferably animal feed and aquafeed. Additionally, or alternatively, the fungal biomass is essentially free from solids that are insoluble in a feedstock comprising one or more dissolved carbon sources, preferably wherein one of the one or more dissolved carbon sources is furfural. Alternatively, or additionally, the fungal biomass comprises <1.0 wt%, preferably <0.5 wt%, more preferably 0 wt%, of solids that are insoluble in a feedstock, based on the total weight of the feedstock comprising one or more dissolved carbon sources, preferably said solids that are insoluble may have a size of >1.0 µm, preferably >0.5 µm, more preferably >0.2 µm. Alternatively, or additionally, the fungal biomass contains <1.0 wt%, preferably <0.5 wt%, more preferably 0 wt%, of solids that are insoluble in a feedstock, based on the total weight of the feedstock comprising one or more dissolved carbon sources, preferably said solids that are insoluble may have a size of >1.0 µm, preferably >0.5 µm, more preferably >0.2 µm. These fungal biomasses may be less toxic to aquatic organisms and humans. Additionally, or alternatively, the fungal biomass is edible. Therefore, these fungal biomasses are safe for animals and / or humans to eat and may be used as or in foodstuff such as, but not limited to, food for consumption of humans, animal feed, fodder, and / or compound feed, preferably as or in fish feed and / or aquafeed. Preferably, the foodstuff is fish feed or aquafeed. Additionally, or alternatively, the fungal biomass is foodstuff. Alternatively, the fungal biomass is animal feed. Preferably, the animal feed is fish feed or aquafeed. Additionally, or alternatively, the fungal biomass is obtainable by a method for the continuous preparation of the fungal biomass as disclosed in the present disclosure. Additionally, or alternatively, the fungal biomass comprising one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae, wherein the fungal biomass has a crude protein content of at least 56 %, based on the total dry weight of the fungal biomass, and wherein the one or more fatty acids comprises one or more polyunsaturated fatty acids, wherein the content of the one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids, is obtainable by a method for the continuous preparation of the fungal biomass, comprising: i) providing one or more filamentous fungi each inde- pendently selected from the fungal family Tricho- comaceae, Thermoascaceae, and / or Aspergillaceae; ii) combining a feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, with the one or more filamentous fungi; iii) cultivating the combined the one or more fila- mentous fungi and the feedstock under aerobic condi- tions to form the fungal biomass; and iv) collecting formed fungal biomass from the feed- stock, wherein the dilution rate is 0.3 h-1or less. Addition- ally, or alternatively, in ii), the one or more dissolved carbon sources content of the feedstock is 0.5 – 10 wt%, preferably 2 – 4 wt%, more preferably 2 – 3 wt%. It is to be understood that the terms “one or more dissolved carbon sources content” refers to the total content of the one or more dissolved carbon sources content. Additionally, or alternatively, in ii), the concentration of fur- fural in the feedstock is selected from 30 - 100 wt% or 30 – 90 wt%, based on the total dry weight of the one or more dissolved carbon sources. Additionally, or alternatively, the feedstock com- prising one or more dissolved carbon sources is essentially free from insoluble solids. Additionally, or alternatively, the feed- stock comprises <1.0 wt%, preferably <0.5 wt%, more preferably 0 wt%, of solids that are insoluble in the feedstock, based on the total weight of the feedstock comprising one or more dissolved carbon sources, preferably said solids that are insoluble may have a size of >1.0 µm, preferably >0.5 µm, more preferably >0.2 µm. Additionally, or alternatively, the feedstock contains <1.0 wt%, preferably <0.5 wt%, more preferably 0 wt%, of solids that are insoluble in the feedstock, based on the total weight of the feed- stock comprising one or more dissolved carbon sources, preferably said solids that are insoluble may have a size of >1.0 µm, pref- erably >0.5 µm, more preferably >0.2 µm. Even more preferably, the feedstock is free from insoluble solids. Additionally, or alter- natively, the one or more filamentous fungi is Paecilomyces vari- otii strain KCL-24. Additionally, or alternatively, in iii) cul- tivating comprises agitating the combined one or more filamentous fungi and the feedstock at 1000 - 1400 rpm, or at ca. 1200 rpm, at a temperature of 35 - 40 °C, preferably 37 °C, at a pH of 4.5 – 5.0, and with an aeration of 0.1 - 0.3 VVM (volume per volume minute), preferably 0.3 VVM. Additionally, or alternatively, the formed fungal biomass has a crude protein content that is selected from 56-74 %, 61-74 %, and 65-74 %, based on the total dry weight of the fungal biomasses. Additionally, or alternatively, the formed fungal biomass has a content of linoleic acid that is at least 69 wt%, or it is about 70 wt% or 69 – 70 wt%, based on the total weight of the one or more fatty acids. In one aspect is disclosed a method for the continuous preparation of a fungal biomass, comprising: i) providing one or more filamentous fungi each inde- pendently selected from the fungal family Tricho- comaceae, Thermoascaceae, and / or Aspergillaceae; ii) combining a feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, with the one or more filamentous fungi; iii) cultivating the combined one or more filamentous fungi and the feedstock under aerobic conditions to form the fungal biomass; and iv) collecting formed fungal biomass from the feed- stock, wherein the dilution rate is 0.3 h-1 or less. Methods for the continuous preparation of a fungal biomass as disclosed herein and hereafter enables the preparation of fungal biomasses with high crude protein content. A crude protein content of at least 56 % based on the total dry weight of the fungal biomass is considered a high protein content. Additionally, the methods enable an efficient preparation of fungal biomasses from various feedstocks comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural. A person skilled in the art understands that dilution rate includes fresh feedstock is continuously added at the same rate as continuously collecting the formed fungal biomass to keep the volume of the combined one or more filamentous fungi and the feedstock in ii), i.e., the culture volume, constant. It has surprisingly been found that methods for the continuous preparation of fungal biomasses using feedstocks comprising furfural, or supplementation of furfural to the continuous fermentation for mycoprotein production (continuous preparation of fungal biomass) may significantly increase the crude protein content in the resulting fungal biomass (mycoprotein ingredient). Furthermore, it has surprisingly been found that using furfural in a method for the continuous preparation of fungal biomass as disclosed in the present disclosure may significantly increase the content of one or more polyunsaturated fatty acids in the resulting fungal biomass, in addition to the increase of the crude protein content in the resulting fungal biomass. Furthermore, it has surprisingly been found that the methods for the continuous preparation of fungal biomass (i.e., nutrients in mycoprotein ingredients) as disclosed in the present disclosure may be used to prepare fungal biomass having an increased crude protein content and / or an increased content of one or more polyunsaturated fatty acids using lower dilution rates (0.3 h-1or less), which in turn may be necessary when feedstocks containing less preferable carbon sources, such as furfural, are used. On the other hand, furfural may be a product of the dehydration of xylose, an abundantly available pentose sugar in lignocellulosic biomass resources, and may be formed as a by-product in pre-treatment processes for the hydrolysis of lignocellulosic biomass. Therefore, furfural as such may be a potential carbon source in low-value side streams from the biorefining of lignocellulosic biomass, opening new uses of these low-value side streams. Furthermore, due to high crude protein content formed by the continuous preparation method, the production cost of protein of fungal biomass formed by the method is lower compared to conventional methods for preparing protein of fungal biomasses that result in lower crude protein contents of the biomasses. In addition, the continuous preparation combined with the dilution rate (h-1) according to methods as disclosed herein and hereafter may provide both an increased productivity and crude protein content of the formed biomass. Methods for continuous preparation of fungal biomasses as disclosed herein and hereafter enables continuous collecting, e.g., by filtrating, of the formed fungal biomass and, therefore, compared to, e.g., batch process of fungal biomass, provides an increased productivity since interruptions in the preparation of fungal biomass may be avoided. Additionally, methods as disclosed herein and hereafter comprising the use of filamentous fungus, e.g., Paecilomyces variotii strain KCL-24, may enable easier and / or cheaper collecting of the formed fungal biomass, since compared to conventional methods for preparation of fungal biomass utilizing centrifugation to collect formed biomass, the collecting the formed fungal biomass in the methods as disclosed herein and hereafter may be performed by simply filtering the formed fungal biomass from the feedstock. This may have a favorable impact on productivity. Additionally, or alternatively, the iii) cultivating the combined one or more filamentous fungi and the feedstock under aerobic conditions to form the fungal biomass is performed in a bioreactor. Additionally, or alternatively, the bioreactor is selected from a stirred tank, airlift, and any system suitable for cultivation of one or more filamentous fungi to form the fungal biomass. Additionally, or alternatively, the feedstock comprises water and one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural. Additionally, or alternatively, ii) comprises adding one or more carbon sources to the feedstock before or during the feedstock is combined with the one or more filamentous fungi. Therefore, it is to be understood that the feedstock may or may not comprise furfural before adding one or more carbon sources to the feedstock, but at least furfural as a carbon source is added to the feedstock if the feedstock is free of furfural. Additionally, or alternatively, the one or more carbon sources being added in ii) to the feedstock before or during the feedstock is combined with the one or more filamentous fungi is one or more carbon sources which(that) dissolve(s) in the feedstock. Additionally, or alternatively, ii) comprises adding one or more carbon sources to the feedstock before or during the feedstock is combined with the one or more filamentous fungi, wherein the one or more carbon sources are each independently selected form the group consisting of furfural, xylose, acetate, and formate. Additionally, or alternatively, the feedstock is essentially free from insoluble solids that have a size of at least 1.0 µm, preferably at least 0.5 µm, more preferably at least 0.2 µm. Additionally, or alternatively, the feedstock comprises <1.0 wt%, preferably <0.5 wt%, more preferably 0 wt%, of solids that are insoluble in the feedstock, based on the total weight of the feedstock comprising one or more dissolved carbon sources, preferably said solids that are insoluble may have a size of >1.0 µm, preferably >0.5 µm, more preferably >0.2 µm. Additionally, or alternatively, the feedstock contains <1.0 wt%, preferably <0.5 wt%, more preferably 0 wt%, of solids that are insoluble in the feedstock, based on the total weight of the feedstock comprising one or more dissolved carbon sources, preferably said solids that are insoluble may have a size of >1.0 µm, preferably >0.5 µm, more preferably >0.2 µm. It has surprisingly been found that when the feedstock is essentially free from insoluble solids high crude protein content of fungal biomass is formed in the methods disclosed herein and hereafter. In addition, when the feedstock is essentially free from insoluble solids with a particle size of at least 1.0 µm, at least 0.5 µm, or at least 0.2 µm, collecting of formed biomass is easier since insoluble solids with a particle size of at least 1.0 µm, at least 0.5 µm, or at least 0.2 µm may negatively clog the collecting apparatus or have a negative impact on filtering properties of the biomass. In addition, the collected formed biomass may lack insoluble solids that may be toxic or may bring undesirable properties to the biomass. Additionally, or alternatively, ii) comprises removing, at least partially, insoluble solids from the feedstock, preferably wherein the insoluble solids have a size of at least 1.0 µm, more preferably at least 0.5 µm, even more preferably at least 0.2 µm, before the feedstock is combined with the one or more filamentous fungi. More preferably, removing insoluble solids having a size of at least 1.0 µm, preferably at least 0.5 µm, more preferably at least 0.2 µm, from the feedstock, preferably removing >50 %, >70 %, >80 %, >90 %, >95 %, >97 %, or >99 % of the solids that are insoluble in the feedstock from the feedstock. Even more preferably, removing 100 % of the solids that are insoluble in the feedstock from the feedstock. These methods enable the continuous preparation of fungal biomasses, wherein the crude protein content of formed fungal biomass of step iv) is at least 56 wt%, or 56 - 74 %, 61 - 74 %, or 65 - 74 %, based on the total dry weight of the fungal biomasses. Additionally, or alternatively, formed fungal biomass of step iv) has a content of one or more polyunsaturated fatty acids (such as linoleic acid) that is at least 56 wt%, or at least 69 wt%, or it is about 70 wt% or 69 – 70 wt%, based on the total weight of the one or more fatty acids. Preferably, removing is selected from the group consisting of filtering, clarificating, decantating, settling, centrifugating, and screening. Preferably, after removing, at least partially, insoluble solids from the feedstock, the feedstock is essentially free from insoluble solids that have a size of at least 1.0 µm, more preferably at least 0.5 µm, more preferably at least 0.2 µm. As disclosed above, removing insoluble solids from the feedstock may form a fungal biomass with a high crude protein content may improve the collecting of formed biomass, and may eliminate potential toxic, harmful and / or unwanted insoluble solids ending up in the formed biomass. Additionally, or alternatively, the method for the continuous preparation of a fungal biomass further comprises diluting or concentrating the feedstock, preferably diluting the feedstock. More preferably, diluting is adding water to the feedstock. It is to be understood that diluting or concentrating the feedstock may be performed before and / or during ii) and / or iii). It has surprisingly been found that by diluting or concentrating the feedstock comprising the one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, to a carbon sources content of 0.5-10 wt%, preferably 2-4 wt%, more preferably 2-3 wt%, the formed fungal biomass has a crude protein content of at least 56 % based on the total dry weight of the fungal biomass. In addition, it has surprisingly been found that by adjusting the concentration of carbon sources of the feedstock the productivity (g (protein)L-1h-1) of the formed biomass may be increased, without wasting carbon sources. Additionally, or alternatively, methods for the continuous preparation of a fungal biomass comprise after, before, or during iv): v) adding a fresh feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, to the combined one or more filamentous fungi and the feedstock. It is to be understood that typically in methods for the continuous preparation of a fungal biomass the volume of fresh feedstock being added in v) may be the same as the volume of formed fungal biomass being collected in iv). E.g., when the dilution rate is 0.3 h-1in a method for the continuous preparation of a fungal biomass as disclosed herein and hereafter, fresh feedstock is being added in v) having a volume that may correspond to 0.3 times the volume of the combined feedstock and the one or more filamentous fungi in ii) every hour, and formed fungal biomass is being collected from the feedstock in iv) having a volume that may be 0.3 times the volume of the combined feedstock and the one or more filamentous fungi in ii) every hour, therefore, the culture volume, i.e. the total volume of feedstock (including fresh feedstock) and one or more filamentous fungi, may be constant. Preferably, the fresh feedstock comprising one or more dissolved carbon sources is essentially free from insoluble solids. Additionally, or alternatively, prior to v), insoluble solids are removed, at least partially, from the fresh feedstock. Removing may be, but not limited to, filtering, clarificating, decantating, settling, centrifugating, and / or screening. It is to be understood that said fresh feedstock (or aqueous culture medium) may be feedstock (or aqueous culture medium), wherein the one or more filamentous fungi has not been cultivated in. Additionally, or alternatively, the method for the continuous preparation of a fungal biomass is a method for the continuous preparation of a fungal biomass as disclosed herein and hereafter. Additionally, or alternatively, the method for the continuous preparation of a fungal biomass further comprises sterilizing the feedstock prior to combining the feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, with the one or more filamentous fungi. Additionally, or alternatively, in iv), formed fungal biomass is collected from the feedstock when the formed fungal biomass has a crude protein content of at least 56 %, preferably 56 - 74 %, more preferably 60 –74 %, even more preferably 61 –74 %, based on the total dry weight of the fungal biomass. Additionally, or alternatively, in iv), formed fungal biomass is collected from the feedstock when the content of at least one of one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of one or more fatty acids comprised in the fungal biomass. Additionally, or alternatively, in iv), formed fungal biomass is collected from the feedstock when the content of at least one of one or more polyunsaturated fatty acids is at least 56 wt%, or it is 56 – 90 wt%, 56 – 85 wt%, 56 – 80 wt%, 56 – 70 wt%, 60 – 90 wt%, 60 – 85 wt%, 60 – 80 wt%, 60 – 75 wt%, 60 – 70 wt%, 65 – 90 wt%, 65 – 85 wt%, 65 – 80 wt%, 65 – 75 wt%, 65 – 70 wt%, 69 – 70 wt%, or at least 69 wt%, or it is about 70 wt%, based on the total weight of the one or more fatty acids comprised in the fungal biomass. Additionally, or alternatively, in iv), formed fungal biomass is collected from the feedstock when the total content of one or more polyunsaturated fatty acids is at least 56 wt%, or it is 56 – 90 wt%, 56 – 85 wt%, 56 – 80 wt%, 56 – 70 wt%, 60 – 90 wt%, 60 – 85 wt%, 60 – 80 wt%, 60 – 75 wt%, 60 – 70 wt%, 65 – 90 wt%, 65 – 85 wt%, 65 – 80 wt%, 65 – 75 wt%, 65 – 70 wt%, 69 – 70 wt%, or at least 69 wt%, or it is about 70 wt%, based on the total weight of the one or more fatty acids comprised in the fungal biomass. Additionally, or alternatively, the at least one of one or more polyunsaturated fatty acids is linoleic acid. Additionally, or alternatively, in iv), formed fungal biomass is collected from the feedstock when the content of linoleic acid is at least 56 wt%, or it is 56 – 90 wt%, 56 – 85 wt%, 56 – 80 wt%, 56 – 70 wt%, 60 – 90 wt%, 60 – 85 wt%, 60 – 80 wt%, 60 – 75 wt%, 60 – 70 wt%, 65 – 90 wt%, 65 – 85 wt%, 65 – 80 wt%, 65 – 75 wt%, 65 – 70 wt%, 69 – 70 wt%, or at least 69 wt%, or it is about 70 wt%, based on the total weight of the one or more fatty acids comprised in the fungal biomass. Additionally, or alternatively, the one or more filamentous fungi is each independently selected from the fungal genera Paecilomyces, Gliocladium, Trichoderma, Byssochlamys, Spicaria, Aspergillus, Penicillium, Rasamsonia, Talaromyces, and Thermoascus. These methods are advantageous since fungal biomasses having a high crude protein content and a high content of one or more polyunsaturated fatty acids may be prepared, and easy collecting formed fungal biomass from the feedstock may be achieved. Additionally, or alternatively, the one or more filamentous fungi is each independently selected from the fungal species Paecilomyces variotii, Paecilomyces puntonii, Gliocladium virens, Trichoderma viride, Byssochlamys nivea, Spicaria divaricata, Aspergillus niger, and Aspergillus oryzae. These methods are advantageous since fungal biomasses having a high crude protein content and a high content of one or more polyunsaturated fatty acids may be prepared, and easy collecting formed fungal biomass from the feedstock may be achieved. Additionally, or alternatively, the one or more filamen- tous fungi is Paecilomyces variotii strain KCL-24. It has surpris- ingly been found that using Paecilomyces variotii strain KCL-24 in a method disclosed herein and hereafter fungal biomass is formed that has a high crude protein content, i.e., a crude protein con- tent of at least 56 % based on the total dry weight of the fungal biomass. In addition, Paecilomyces variotii strain KCL-24 forms fungal biomass with a suitable amino acid composition for food- stuff, a good protein digestibility in animals, and lack mycotox- ins. Furthermore, it has been surprisingly found that Paecilomyces variotii strain KCL-24 is suitable in a method for continuous preparation of fungal biomasses, wherein the dilution rate is 0.3 h-1or less. The strain also enables a high productivity of fungal biomass and collecting the fungal biomass formed by Paecilomyces variotii strain KCL-24 is easier than biomass of non-filamentous fungi. Furthermore, it has surprisingly been found that using Pae- cilomyces variotii strain KCL-24 in a method disclosed herein and hereafter fungal biomass is formed that has a high content of at least one of one or more polyunsaturated fatty acids, such as linoleic acid, wherein the content of at least one of one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of one or more fatty acids comprised in the fungal biomass. Additionally, or alternatively, the dilution rate is se- lected from 0.05-0.30 h-1, 0.1-0.30 h-1, 0.10-0.20 h-1, 0.05-0.25 h-1, 0.05-0.20 h-1, 0.10 h-1, 0.15 h-1, 0.20 h-1, 0.25 h-1, and 0.30 h-1. It has surprisingly been found that these dilution rates in continuous preparations of fungal biomasses according to methods disclosed herein and hereafter form fungal biomasses having a high crude protein content, i.e., a crude protein content of at least 56 %, based on the total dry weight of the fungal biomass. There- fore, high (> 0.3 h-1) dilution rates can be avoided in these methods when fungal biomass with high crude protein content is required. High dilution rates may result in suboptimal carbon sources utilization and part of the carbon sources may leak out of the production process and remain unused. With some feedstocks, a high dilution rate may result in a too low biomass concentration or a complete leak-out of the fungus cultivated in the bioreactor. Therefore, optimal carbon source utilization and decent biomass concentration may be achieved with methods as disclosed in the present disclosure. Additionally, or alternatively, the dilution rate com- prises a plurality of dilution rates, wherein each of the plurality of dilution rates is independently selected from 0.05-0.30 h-1, 0.1-0.30 h-1, 0.10-0.20 h-1, 0.05-0.25 h-1, 0.05-0.20 h-1, 0.10 h-1, 0.15 h-1, 0.20 h-1, 0.25 h-1, and 0.30 h-1. It is to be understood that the dilution rate may be changed one or more times during the method for continuous preparation of the fungal biomass as dis- closed in the present disclosure. For example, first a dilution rate of 0.20 h-1may be used and subsequently a dilution rate of 0.10 h-1may be used, or first a dilution rate of 0.15 h-1may be used and subsequently a dilution rate of 0.20 h-1may be used. Additionally, or alternatively, after optionally removing insoluble solids from the feedstock and / or optionally diluting or concentrating the feedstock, in ii), the one or more dissolved carbon sources content of the feedstock is 0.5-10 wt%, preferably 2-4 wt%, more preferably 2-3 wt%. It is to be understood that the terms “the one or more dissolved carbon sources content of the feedstock” as used herein and hereafter may refer to the total content of the one or more dissolved carbon sources of the feedstock. It has surprisingly been found that using a feedstock having a one or more dissolved carbon sources content of 0.5-10 wt%, preferably 2-4 wt%, more preferably 2-3 wt%, the crude protein content of the formed fungal biomass may be increased, wherein the crude protein content of the formed fungal biomass is at least 56, or 56 - 74 %, 61 - 74 %, or 65 - 74 %, based on the total dry weight of the fungal biomasses. In addition, it has surprisingly been found that the effect of the one or more dissolved carbon sources content being 0.5-10 wt%, preferably 2-4 wt%, more preferably 2-3 wt%, is the carbon sources being effectively utilized by the one or more filamentous fungi in a method as disclosed herein and hereafter. Carbon sources contents >10 wt% may not be utilized by the one or more filamentous fungi, thereby wasting carbon sources and making the preparation method less effective / profitable, i.e., decreasing productivity of biomass. Carbon sources contents <0.5 wt% may be a limiting factor in the cultivation to form fungal biomass, thereby negatively affecting the productivity (g (protein)L-1h-1) of the formed biomass. Additionally, or alternatively, in ii), the concentration of furfural in the feedstock is selected from 30 – 100 wt%, based on the total dry weight of the one or more dissolved carbon sources. It is to be understood that “concentration of furfural in the feedstock” in the present disclosure refers to the concentration of furfural in the feedstock before and / or during the feedstock is being used in methods for the preparation of fungal biomass as disclosed in the present disclosure, i.e., before and / or during the feedstock is being used by the one or more filamentous fungi. Additionally, or alternatively, in ii), the concentration of furfural in the feedstock is selected from at least 5 wt%, at least 10 wt%, at least 15 wt%, at least 20 wt%, at least 25 wt%, at least 30 wt%, at least 35 wt%, at least 40 wt%, at least 44 wt%, at least 50 wt%, at least 70 wt%, at least 80 wt%, at least 90 wt%, 40 – 100 wt%, 40 – 90 wt%, 50 – 90 wt%, 60 – 90 wt%, 70 – 90 wt%, 80 – 90 wt%, about 40 wt%, about 50 wt%, about 67 wt%, about 75 wt%, and about 88.9 wt%, based on the total dry weight of the one or more dissolved carbon sources. It has been surprisingly found that with these methods a high protein content, based on the total dry weight of the fungal biomass, of formed fungal biomass may be achieved. Additionally, it has been surprisingly found that a high content (i.e., at least 56 wt%) of one or more polyunsaturated fatty acids in formed fungal biomass, based on the total weight of the one or more fatty acids, may be achieved with these methods, in particular a high content of at least one omega-6 fatty acid of formed fungal biomass, based on the total weight of the one or more fatty acids, may be achieved, more in particular a high content of linoleic acid of formed fungal biomass, based on the total weight of the one or more fatty acids, may be achieved. Additionally, or alternatively, a decreased content of one or more saturated fatty acid of formed fungal biomass, based on the total weight of the one or more fatty acids, may be achieved with these methods. Additionally, or alternatively, in ii), the feedstock comprises two or more dissolved carbon sources, wherein two dissolved carbon sources of the two or more dissolved carbon sources are furfural, and xylose. Additionally, or alternatively, in ii), the feedstock comprises two or more dissolved carbon sources, wherein two dissolved carbon sources of the two or more dissolved carbon sources are furfural, and xylose, and the total concentration of furfural and xylose in the feedstock is 10 – 30 g / L, 18 – 23 g / L, or ca. 20 g / L. Additionally, or alternatively, in ii), the feedstock comprises two or more dissolved carbon sources, wherein two dissolved carbon sources of the two or more dissolved carbon sources are furfural, and at least one of saccharose, glucose, and fructose. Additionally, or alternatively, in ii), the feedstock comprises two or more dissolved carbon sources, wherein two dissolved carbon sources of the two or more dissolved carbon sources are furfural, and at least one of saccharose, glucose, and fructose, the concentration of furfural in the feedstock is 4 — 30 g / L, 8 – 30 g / L, 10 – 30 g / L, 13 – 30 g / L, 15 – 30 g / L, 17 – 30 g / L, 18 – 23 g / L, or ca. 20 g / L, and the total concentration of saccharose, glucose, and / or fructose is 0 – 10 wt%, based on the total dry weight of the two or more dissolved carbon sources. With these methods an increased protein content of formed fungal biomass may be achieved. Additionally, or alternatively, an increased content of at least one of one or more polyunsaturated fatty acids in formed fungal biomass may be achieved, in particular an increased content of at least one omega- 6 fatty acid of formed fungal biomass may be achieved, more in particular an increased content of linoleic acid of formed fungal biomass may be achieved. Additionally, or alternatively, a decreased content of one or more saturated fatty acid of formed fungal biomass may be achieved. Additionally, or alternatively, the concentration of xylose is less than 90 wt%, based on the total dry weight of the two or more dissolved carbon sources. It is to be understood that when the concentration of xylose is less than 90 wt% the concentration of furfural may, e.g., be more than 0 wt%, more than 0 and up to 100 wt%, more than 10 wt% and up to 100 wt%, or more than 0 wt% and up to 90 wt%, based on the total dry weight of the two or more dissolved carbon sources, and the total concentration of the rest of the two or more dissolved carbon sources (if present) adds up to 100 wt%, based on the total dry weight of the two or more dissolved carbon sources. E.g., when the feedstock comprises two or more dissolved carbon sources, wherein two dissolved carbon sources of the two or more dissolved carbon sources are furfural, and xylose, wherein the concentration of xylose is 50 wt%, the concentration of furfural in the feedstock may be 40 wt%, both based on the total dry weight of the two or more dissolved carbon sources, and the feedstock further comprises 10 wt% of one or more dissolved carbon sources being different to xylose and furfural, such as saccharose, based on the total dry weight of the dissolved carbon sources. With these methods an increased protein content of formed fungal biomass and / or an increased content of at least one of one or more polyunsaturated fatty acids in formed fungal biomass may be achieved. Additionally, or alternatively, the concentration of xylose is < 85 wt%, < 80 wt%, < 75 wt%, < 70 wt%, < 65 wt%, < 60 wt%, < 46 wt%, < 40 wt%, < 20 wt%, < 10 wt%, < 5 wt%, 0 wt%, 0 – 60 wt%, 0 – 50 wt%, 0 – 40 wt%, 0 – 30 wt%, 0 – 20 wt%, 0 – 10 wt%, about 67 wt%, about 50 wt%, about 46 wt%, about 40 wt%, about 23 wt%, and about 15 wt%, based on the total dry weight of the one or more dissolved carbon sources. With these methods an increased protein content of formed fungal biomass and / or an increased content of at least one of one or more polyunsaturated fatty acids in formed fungal biomass may be achieved. Additionally, or alternatively, the method comprises changing one or more times the feedstock being used in the method, wherein each of the feedstocks comprises one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources of each of the feedstocks is furfural. It is to be understood that the feedstock being used in the method for continuous preparation of fungal biomass as disclosed in the present disclosure may be changed, during the method, one or more times, from one feedstock to another feedstock, as long as the feedstocks used comprise furfural. For example, first a first feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, wherein the concentration of furfural in the first feedstock is 50 wt%, based on the total dry weight of the one or more dissolved carbon sources, is used, and then a second feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, wherein the concentration of furfural in the second feedstock is about 90 wt%, based on the total dry weight of the one or more dissolved carbon sources, is used (and optionally further (third, fourth, fifth (and so on)) feedstock(s) comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, wherein the concentration of furfural in the further feedstock(s) is selected from 5 – 100 wt%, based on the total dry weight of the one or more dissolved carbon sources, is used). These methods are beneficial, since fungal biomasses having different crude protein contents (being at least 56 %, based on the total dry weight of the fungal biomass) and different contents of the one or more polyunsaturated fatty acids (being at least 56 wt%, based on the total weight of the one or more fatty acids) may be prepared. It is to be noted that, additionally to changing the feedstock, the dilution rate may be changed during the method for continuous preparation of the fungal biomass as disclosed in the present disclosure. Additionally, or alternatively, in ii), the feedstock comprises three or more dissolved carbon sources, wherein three dissolved carbon sources of the three or more dissolved carbon sources are furfural, acetate, and formate, preferably the concentration of furfural in the feedstock is at least 76 wt%, based on the total dry weight of the one or more dissolved carbon sources. Additionally, or alternatively, in ii), the concentration of furfural in the feedstock is at least or about 4.15 g / L, the concentration of acetate in the feedstock is at least or about 3.04 g / L, and the concentration of formate in the feedstock is at least or about 0.78 g / L. With these methods an increased protein content of formed fungal biomass and / or an increased content of at least one of one or more polyunsaturated fatty acids in formed fungal biomass may be achieved. Additionally, or alternatively, the feedstock is selected from a thin stillage, vinasse, molasses, spent sulphite liquor, prehydrolysis liquor, food industry processing waste, and biorefinery by-product, or any mixture or combination thereof. It should be understood that these feedstocks typically comprise one or more dissolved carbon sources, and if the feedstocks do not comprise furfural before the feedstock is combined with the one or more filamentous fungi in ii), furfural may be added to the feedstock before or during the feedstock is combined with the one or more filamentous fungi in ii). Additionally, or alternatively, the feedstock further comprises one or more dissolved carbon sources each independently selected from carbohydrates, carbohydrate derivatives, aldehydes, sugars, oligosaccharides, polysaccharides, polyols, carboxylic acids, sugar acids, and alcohols, or any combinations thereof. Additionally, or alternatively, the carbohydrates is each independently selected from glucose, mannose, xylose, arabinose, galactose, fructose, sucrose, maltose, isomaltulose, trehalose, lactose, maltotriose, maltodextrins, xylooligosaccharides (XOS), raffinose, stachyose, fructo-oligosaccharides; the polyols is each independently selected from glycerol, mannitol, sorbitol; the carboxylic acids is each independently selected from formic acid, formate, acetic acid, acetate, lactic acid, propionic acid, aldonic acid, ulosonic acids, uronic acid, aldaric acid; the alcohols is each independently selected from methanol, ethanol, and ethylene glycol; or any combinations thereof. Preferably, the feedstock further comprises one or more one or more dissolved carbon sources is each independently selected from sucrose (saccharose), glucose, fructose, maltodextrins, xylose, mannose, glycerol, acetic acid, lactic acid, and formic acid, or combinations thereof. Additionally, or alternatively, in ii), the feedstock comprises three or more dissolved carbon sources, wherein three dissolved carbon sources of the three or more dissolved carbon sources are furfural, xylose, and saccharose. Additionally, or alternatively, in ii), the feedstock comprises three or more dissolved carbon sources, wherein three dissolved carbon sources of the three or more dissolved carbon sources are furfural, xylose, and saccharose, and the total concentration of furfural and xylose in the feedstock is 10 – 30 g / L, 18 – 23 g / L, or ca. 20 g / L, preferably the concentration of furfural in the feedstock is at least 76 wt%, based on the total dry weight of the three or more dissolved carbon sources. With these methods an increased protein content and / or increased content of one or more polyunsaturated fatty acids of formed fungal biomass may be achieved. Additionally, or alternatively, in ii), the feedstock is molasses, wherein the feedstock comprises four or more dissolved carbon sources, wherein four dissolved carbon sources of the four or more dissolved carbon sources are furfural, acetate, formate, and saccharose, preferably the concentration of furfural in the feedstock is at least 76 wt%, based on the total dry weight of the four or more dissolved carbon sources. With these methods an increased protein content and / or increased content of one or more polyunsaturated fatty acids of formed fungal biomass may be achieved. Additionally, or alternatively, the method further comprises drying the fungal biomass after iv) collecting formed fungal biomass from the feedstock. Additionally, or alternatively, the drying is performed in two or more steps each independently selected from drying using hot air, filtering, freeze drying, indirect or contact drying; and natural air drying, or any combination thereof. Preferably, the drying is filtering and drying using hot air. Preferably, the fungal biomass is dried until the fungal biomass has a water content of 0.5-10 wt%, preferably 3-8 wt%, more preferably 4-7 wt%, based on the total weight of the fungal biomass. With these methods, a longer shelf life of the fungal biomass may be achieved. Additionally, or alternatively, the aqueous culture medium or the feedstock further comprises one or more organic and / or inorganic compounds each independently selected from the group consisting of aldehydes, carbohydrates, carboxylic acids, nitrogen supplementation compounds, antifoaming agents, phosphorus supplementation compounds, trace elements, and inorganic salts. In embodiments, the one or more organic and / or inorganic compounds is each independently selected from the group consisting of furfural, carbohydrates, formate, acetate, NH4OH, (NH4)2SO4, CH4N2O, KH2PO4, (NH4)2HPO4, H3PO4, phosphates, CaCl2, KCl, MgSO4, Fe2(SO4)3, Fe(NH4)2(SO4)2, ZnSO4, CuSO4, MnSO4, HCl, H3BO4, Na2MoO4, Vogel's trace elements, Struktol J673A, citric acid, and any salts, hydrates and combinations thereof. Additionally, or alternatively, the method for the continuous preparation of a fungal biomass further comprises after, before, or during ii), iii), and / or iv): - adding one or more organic and / or inorganic compounds each independently selected from the group consisting of aldehydes, carbohydrates, carboxylic acids, nitrogen supplementation compounds, antifoaming agents, phosphorus supplementation compounds, trace elements, and inorganic salts to the aqueous culture medium or the feedstock. In embodiments, the one or more organic and / or inorganic compounds are each independently selected from the group consisting of furfural, carbohydrates, formate, acetate, NH4OH, (NH4)2SO4, CH4N2O, KH2PO4, (NH4)2HPO4, H3PO4, phosphates, CaCl2, KCl, MgSO4, Fe2(SO4)3, Fe(NH4)2(SO4)2, ZnSO4, CuSO4, MnSO4, HCl, H3BO4, Na2MoO4, Vogel's trace elements, an antifoaming agent such as Struktol J673A; citric acid, and any salts, hydrates and combinations thereof. Additionally, or alternatively, the feedstock is selected from a thin stillage, molasses, vinasses, and other feedstocks comprising one or more dissolved carbon sources, wherein the feedstock further comprises one or more dissolved carbon sources each independently selected from the group consisting of glycerol, acetic acid, formic acid, sucrose (saccharose), glucose, fructose, maltodextrins, xylose, mannose, and lactic acid, or any combinations or mixtures thereof; wherein the feedstock further comprises (NH4)2SO4, KH2PO4, CaCl2, Vogel’s trace elements, and an antifoaming agent, preferably, Struktol J673A. Additionally, or alternatively, the method further comprises adjusting the pH of the combined one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae and feedstock. Preferably, adjusting the pH to 3.0-6.0, more preferably to 4.5-5.0. Additionally, or alternatively, in iii), the cultivating comprises adjusting the pH to, and / or maintaining the pH at, 3.0- 6.0, more preferably 4.5-5.0. Additionally, or alternatively, in iii), the pH during the cultivating is 4.5-5.0. Compounds that may be used to adjust the pH is not particular limited to any specific compounds but may be (NH4)2SO4, KH2PO4, NH4OH, and / or H3PO4, or any combinations or mixtures thereof. Additionally, or alternatively, the cultivating the combined the one or more filamentous fungi and the feedstock is performed at 30-45 °C, preferably at 35-41 °C, more preferably at 37-39 °C. Additionally, or alternatively, the aerobic conditions comprise an aeration rate of 0.1-1.0 volume per volume per minute (VVM), preferably 0.1-0.6 VVM, more preferably 0.1-0.3 VVM. Additionally, or alternatively, the combined the one or more filamentous fungi and the feedstock is mixed during the cultivation. Additionally, or alternatively, the method further comprises fractionating the fungal biomass after iv) to form one or more fractions. Alternatively, or additionally, the one or more fractions is at least a fungal protein fraction and / or a β-glucan fraction. Alternatively, or additionally, the cell density of the one or more filamentous fungi is 2 - 20 g / L, preferably 5 - 20 g / L, at steady state during the cultivating the combined the one or more filamentous fungi and the feedstock. The cell density of 2 - 20 g / L, preferably 5 - 20 g / L, enables easier harvesting / collecting of the formed fungal biomass from the feedstock. Additionally, or alternatively, the feedstock comprises two or more feedstocks each comprising one or more dissolved carbon sources, wherein the two or more feedstocks are each independently selected from the group consisting of thin stillage, vinasse, molasses, spent sulphite liquor, prehydrolysis liquor, food industry processing waste, and biorefinery by-product, or any mixture or combination thereof, wherein at least one of the one or more dissolved carbon sources of at least one of the two or more feedstocks is furfural, wherein the rest of the carbon sources are each independently selected from the group consisting of aldehydes, carbohydrates, carbohydrate derivatives, sugars, oligosaccharides, polysaccharides, polyols, carboxylic acids, sugar acids, and alcohols, or any combinations thereof. Alternatively, or additionally, the feedstock is selected from thin stillage, vinasse, molasses, spent sulphite liquor, prehydrolysis liquor, food industry processing waste, and biorefinery by-product, or any mixture or combination thereof; the feedstock further comprises one or more dissolved carbon sources each independently selected from glycerol, acetic acid, acetate, formic acid, formate, sucrose, glucose, fructose, maltodextrins, xylose, mannose, and lactic acid, or combinations thereof; wherein the feedstock is essentially free from insoluble solids; wherein the one or more dissolved carbon sources content of the feedstock is 2-4 wt%; the cell density of the one or more filamentous fungi is 5 - 20 g / L at steady state during the cultivating the combined the one or more filamentous fungi and the feedstock, and the dilution rate is 0.10-0.30 h-1. In embodiments are provided methods for the continuous preparation of a fungal biomass, comprising: i) providing one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae; ii) combining a feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, with the one or more filamentous fungi; iii) cultivating the combined one or more filamentous fungi and the feedstock under aerobic conditions to form the fungal biomass; iv) collecting formed fungal biomass from the feedstock, wherein the dilution rate is 0.3 h-1or less, wherein the feedstock is selected from stillage, thin stillage, vinasse, molasses, spent sulphite liquor, prehydrolysis liquor, food industry processing waste, and biorefinery by-products, or any mixture or combination thereof, and wherein the one or more dissolved carbon sources comprises furfural and at least one or more organic compounds each independently selected from glucose, mannose, xylose, acetate, formate, arabinose, galactose, fructose, sucrose, maltose, isomaltulose, trehalose, lactose, maltotriose, maltodextrins, xylose, xylooligosaccharides (XOS), raffinose, stachyose, fructo-oligosaccharides, glycerol, mannitol, sorbitol, formic acid, acetic acid, lactic acid, propionic acid, methanol, ethanol, ethylene glycol, N- acetylglucosamineglucose, saccharose, and glycerol, wherein the one or more dissolved carbon sources content of the feedstock is 1-4 wt%, preferably 1-3 wt%, wherein the feedstock comprising one or more dissolved carbon sources is essentially free from insoluble solids, and wherein, in iv), the formed fungal biomass is collected from the feedstock when the formed fungal biomass has a crude protein content of 61-75 %, 61-68 %, 62-68 %, 65-68 %, 66-68 %, 61-74 %, 61-66 %, 61-65 %, or 61-62, based on the total dry weight of the fungal biomass. Additionally, or alternatively, in iii), cultivating com- prises agitating the combined one or more filamentous fungi and the feedstock at 1000 - 1400 rpm, or at ca. 1200 rpm. Additionally, or alternatively, in iii), the temperature during the cultivating is selected from the range of 35 - 40 °C, preferably the temperature during the cultivating is 37 °C. Additionally, or alternatively, in iii), cultivating com- prises aeration, wherein the aeration is 0.1 - 0.3 VVM, preferably 0.3 VVM. Additionally, or alternatively, in i), the one or more filamentous fungi is Paecilomyces variotii strain KCL-24; in ii), the feedstock is molasses, wherein the molasses comprises three or more dissolved carbon sources, wherein three dissolved carbon sources of the three or more dissolved carbon sources are furfural, saccharose, and xylose, or wherein the molasses comprises four or more dissolved carbon sources, wherein four dissolved carbon sources of the four or more dissolved carbon sources are furfural, saccharose, acetate, and formate; ammonium sulfate, KH2PO4, CaCl2, MgSO4*7 H2O, and Vogel’s trace elements; the total concentration of the dissolved carbon sources is 10 – 30 g / L, 10 – 23 g / L, 10 – 14 g / L, 18 – 23 g / L, or 20 – 23 g / L; the dilution rate is 0.1 - 0.2 h-1, 0.10 h-1, 0.15 h-1, or 0.20 h-1; in iii), cultivating com- prises agitating the combined one or more filamentous fungi and the feedstock at 1200 rpm, at a temperature selected from the range of 35 - 40 °C, preferably at a temperature of 37 °C, at a pH of 4.5 - 5.0, preferably 5.0, and with an aeration of 0.1 – 0.3 VVM, preferably 0.3 VVM. Additionally, or alternatively, in ii), the feedstock comprises water, 5 g / L molasses, 10 g / L ammonium sulfate, 2 g / L KH2PO4, 0.45 g / L CaCl2, 0.15 g / L MgSO4*7 H2O, 0.1 mL / L Vogel’s trace elements (10000x), furfural, and xylose or acetate and formate, wherein the feedstock comprises 0.5 – 10 wt%, preferably 1 – 4 wt%, dissolved carbon sources, and wherein the concentration of furfural in the feedstock is 1 – 90 wt%, 5 – 90 wt%, 10 – 90 wt%, 15 – 90 wt%, 20 – 90 wt%, 30 – 90 wt%, 40 – 90 wt%, 50 – 90 wt%, 60 – 90 wt%, 70 – 90 wt%, 80 – 90 wt%, 44 – 89 wt%, or about 76.4 wt%, based on the total dry weight of the one or more dissolved carbon sources. In one aspect is disclosed edible compositions comprising fungal biomass as disclosed herein and hereafter. In embodiments the edible composition comprising fungal biomass as disclosed herein and hereafter is fish feed or aquafeed, preferably aquafeed. Edible compositions as disclosed herein and hereafter comprising fungal biomass as disclosed herein and hereafter are beneficial, since all, or at least partially, the SPC that aquafeed and fish feed conventionally comprises, may be replaced by the biomass as disclosed herein and hereafter. Additionally, provided are edible compositions comprising fungal biomass as disclosed herein and hereafter combined with one or more food ingredient, preferably the edible composition is a fish feed or aquafeed, more preferably aquafeed. Additionally, or alternatively, the edible composition is aquafeed and the composition comprises protein originating only from fungal biomass as disclosed herein and hereafter. Additionally, or alternatively, the one or more food ingredient is each independently selected from the group consisting of fishmeal, fish oil, fish feed, soy protein concentrate, soy, soybeans, wheat protein, pea protein, soy protein isolate, wheat protein isolate, pea protein isolate, corn, grain sorghum, oats, rye, barley, food additives, flours, and dairy products, or any combinations thereof. In embodiments, the food additive is selected from the group consisting of acidulants, acidity regulators, anticaking agents, antifoaming and foaming agents, antioxidants, bulking agents, food coloring, fortifying agents, color retention agents, emulsifiers, flavors, flavor enhancers, flour treatment agents, glazing agents, humectants, tracer gas, preservatives, stabilizers, sweeteners, and thickeners, or any combination thereof. In embodiments, the flour is selected from the group consisting of wheat flour, rye flour, fishmeal, farina, and meal, or any combination thereof. In embodiments, the dairy product is selected from the group consisting of milk, yoghurt, curdled milk (soured milk), and cheese, or any combinations thereof. Additionally, or alternatively, the edible composition comprises 10-30 wt% of fungal biomass as disclosed herein and hereafter, preferably 15-27 wt%, more preferably 26-27 wt%, and 70-80 wt% or 70-90 wt% of one or more food ingredient. In embodiments, the total content of soy protein and / or soy protein concentrate (SPC) of the edible composition is ≤20 wt%, preferably ≤4 wt%, more preferably 0 wt%. Additionally, or alternatively, the edible composition comprises 15-27 wt% of the fungal biomass, 9-11 wt% of fishmeal, 4-7 wt% of water, and ≤12 wt%, preferably ≤4 wt%, more preferably 0 wt% soy protein and / or SPC. Additionally, or alternatively, the edible composition comprises 20-30 wt%, preferably 26-27 wt%, of the fungal biomass, 0-11 wt% fishmeal, faba beans 0-5 wt%, wheat gluten 0-12 wt%, sunflower meal 0-1.5 wt%, guar meal 0-3 wt%, fish oil from whole fish 0.5-9.5 wt%, fish oil from trimmings 0-1.5 wt%, micro algal oil 0-0.15 wt%, fish oil from farmed fish 0-0.8 wt%, rapeseed oil 15-25 wt%, camelina oil 0-1.5 wt%, wheat 6-10 wt%, carbohydrates 0-4.6 wt%, and SPC 0-7 wt%, preferably 0-4 wt%, more preferably 0 wt%. In one aspect is disclosed uses of a fungal biomass as disclosed herein or an edible composition as disclosed herein as or in foodstuff. In embodiments is provided use of a fungal biomass as disclosed herein or an edible composition as disclosed herein as or in foodstuff, wherein the foodstuff is selected from the group consisting of food for consumption of humans, compound feed, fodder, and animal feed. In embodiments is provided use of a fungal biomass as disclosed herein or an edible composition as disclosed herein in foodstuff, wherein the foodstuff is fish feed or aquafeed. In another aspect is disclosed uses of a fungal biomass as disclosed herein or an edible composition as disclosed herein as a food ingredient in foodstuff. In embodiments is provided use of a fungal biomass as disclosed herein or an edible composition as disclosed herein as a protein source in fish feed or aquafeed. In another aspect is disclosed uses of a fungal biomass as disclosed herein or an edible composition as disclosed herein as a replacement for soy protein or SPC in foodstuff. In embodiments the foodstuff is aquafeed or fish feed. In another aspect is disclosed use of a method as disclosed in the present disclosure to purify a feedstock, wherein the one or more dissolved carbon sources are, at least partially, removed from the feedstock. The following embodiments are disclosed: 1. A fungal biomass comprising one or more filamentous fungi each independently selected from the fungal family Trichocomaceae; and one or more fatty acids, wherein the fungal biomass has a crude protein content of at least 56 %, based on the total dry weight of the fungal biomass, and wherein the one or more fatty acids comprises one or more polyunsaturated fatty acids, wherein the content of the one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids. 2. The fungal biomass as defined in embodiment 1, wherein the one or more filamentous fungi is each independently selected from the fungal genera Paecilomyces, Gliocladium, Trichoderma, Byssochlamys, Spicaria, Aspergillus, Penicillium, Rasamsonia, Talaromyces, and Thermoascus. 3. The fungal biomass as defined in any preceding embodiment, wherein the one or more filamentous fungi is each independently selected from the fungal species Paecilomyces variotii, Paecilomyces puntonii, Gliocladium virens, Trichoderma viride, Byssochlamys nivea, Spicaria divaricata, Aspergillus niger, and Aspergillus oryzae. 4. The fungal biomass as defined in any preceding embodiment, wherein the one or more filamentous fungi is Paecilomyces variotii strain KCL-24. 5. The fungal biomass as defined in any preceding embodiment, wherein one of the one or more polyunsaturated fatty acids is linoleic acid, wherein the content of linoleic acid is at least 56 wt%, based on the total weight of the one or more fatty acids. 6. A method for the continuous preparation of a fungal biomass, comprising: i) providing one or more filamentous fungi each independently selected from the fungal family Trichocomaceae; ii) combining a feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, with the one or more filamentous fungi; iii) cultivating the combined one or more filamentous fungi and the feedstock under aerobic conditions to form the fungal biomass; and iv) collecting formed fungal biomass from the feedstock, wherein the dilution rate is 0.3 h-1or less. 7. The method as defined in embodiment 6, wherein, in iv), formed fungal biomass is collected from the feedstock when the formed fungal biomass has a crude protein content of at least 56 %, preferably 56 - 74 %, more preferably 61 –74 %, based on the total dry weight of the fungal biomass. 8. The method as defined in any one of embodiments 6 – 7, wherein the one or more filamentous fungi is each independently selected from the fungal genera Paecilomyces, Gliocladium, Trichoderma, Byssochlamys, Spicaria, Aspergillus, Penicillium, Rasamsonia, Talaromyces, and Thermoascus. 9. The method as defined in any one of embodiments 6 – 8, wherein the one or more filamentous fungi is each independently selected from the fungal species Paecilomyces variotii, Paecilomyces puntonii, Gliocladium virens, Trichoderma viride, Byssochlamys nivea, Spicaria divaricata, Aspergillus niger, and Aspergillus oryzae. 10. The method as defined in any one of embodiments 6 – 9, wherein the one or more filamentous fungi is Paecilomyces variotii strain KCL-24. 11. The method as defined in any one of embodiments 6 – 10, wherein, in ii), the one or more dissolved carbon sources content of the feedstock is 0.5 –10 wt%, preferably 2 –4 wt%, more preferably 2 –3 wt%. 12. The method as defined in any one of embodiments 6 – 11, wherein, in ii), the concentration of furfural in the feedstock is selected from 30 - 100 wt%, based on the total dry weight of the one or more dissolved carbon sources. 13. The method as defined in any one of embodiments 6 – 12, wherein the dilution rate is 0.1 – 0.2 h-1. 14. An edible composition comprising fungal biomass as defined in any one of embodiments 1 - 5. 15. Use of a fungal biomass as defined in any one of embodiments 1 –5 or an edible composition as defined in embodiment 14 as or in foodstuff. EXAMPLES Reference will now be made in detail to various embodiments. The description below discloses some embodiments in such a detail that a person skilled in the art is able to utilize the embodiments based on the disclosure. Not all steps or features of the embodiments are discussed in detail, as many of the steps or features will be obvious for the person skilled in the art based on this specification. EXAMPLE 1: Inoculum preparation The fungus Paecilomyces variotii strain KCL-24 was obtained from the VTT Culture Collection. The cultures were maintained on potato dextrose agar (PDA) plates. For preparing mycelium suspension, the mycelium of the fungus Paecilomyces variotii strain KCL-24 was aseptically released using a disposable cell spreader and suspended into 20 mL of sterile water. To prepare fungal biomass inoculum, 5 mL of the mycelium suspension was inoculated into 50 mL of standard medium (table 1). The culture was incubated in a 250-mL shake flask for 24 h at 37 °C and 250 rpm and used as the inoculum in methods for the continuous preparation of fungal biomasses. Table 1. Content of standard medium 1Typically, molasses comprises ca.44 wt% sugars, typically saccharose. EXAMPLE 2: General method for continuous preparations of a fungal biomasses To prepare a continuous bioprocess (continuous preparation) of fungal biomass in a bioreactor, the inoculum (50 mL) prepared in example 1 was aseptically inoculated into 2.95 L of production medium (feedstock) in a stirred tank bioreactor and incubated for 24 h at 37 °C, 1200 rpm, aeration rate 0.3 VVM (volume per volume minute). The production medium contained the feedstock in question as is and diluted with water, nitrogen supplementation with (NH4)2SO4, inorganic compounds, organic compounds, an antifoaming agent such as Struktol J673A, and phosphorus supplementation with, e.g., KH2PO4or H3PO4. After the incubation, the continuous bioprocess was initiated by starting the feeding of production medium (i.e., fresh feedstock) to the bioreactor and collecting suspension comprising formed fungal biomass from the bioreactor at the same rate called the dilution rate. The temperature was kept at 37 °C and the aeration rate was kept at 0.3 VVM. The collected suspension comprising fungal biomass was dewatered (dried) in two steps, first by filtering and subsequently by drying using hot air (50 °C). The crude protein content of the dewatered fungal biomass was analyzed using the Dumas method. EXAMPLE 3: Methods for continuous preparations of fungal biomasses using feedstocks comprising different amounts of furfural as carbon source Method of example 2 was used and the feedstocks as shown in table 2 was used as the production medium, i.e., as feedstock and fresh feedstock. The production media were prepared by adding the substances as shown in table 2 to water and mixing the composition. The continuous fermentation (continuous preparation of fungal biomasses) was started by feeding the feedstock (table 2) comprising 20 g / L xylose that was gradually replaced with furfural over the time (table 3). I.e., in this method for continuous preparation of fungal biomasses, the feedstocks (production media) comprised different amounts of xylose and furfural at different time points, as long as the total amount of added xylose and furfural in the feedstock was 20 g / L. Dilution rate 0.15 h-1was used in this continuous preparation of fungal biomasses. Table 2. Feedstocks (production media) used in Examples. 1Typically, molasses comprises ca.44 wt% sugars, typically saccharose, i.e., 5 g / L of molasses comprises ca. 2.22 g / L of carbon sources, typically saccharose.2The total concentration of added xylose and furfural in the feedstock is given for xylose and furfural. The concentration of furfural of the feedstock was 0 – ca.89 wt%, based on the total dry weight of the dissolved carbon sources, and the concentration of xylose of the feedstock was 0 – ca.89 wt%, based on the total dry weight of the dissolved carbon sources. Increasing the concentration of furfural in the feedstock, and decreasing the concentration of xylose, i.e., increasing the furfural:xylose ratio of the feedstock, results in increased crude protein content of the fungal biomass (table 3). Using a feedstock comprising ca. 89 wt% furfural, based on the total dry weight of the one or more dissolved carbon sources, resulted in a crude protein content (total dry weight basis) of 68% of the fungal biomass (table 3). Table 3. Examples of crude protein contents (%) of fungal biomasses obtained using feedstocks comprising different amounts of furfural and xylose in continuous preparations of fungal biomasses. 1The feedstock used is as defined in table 2 and, therefore, comprises molasses. Typically, molasses comprises ca. 44 wt% sugars, typically saccharose, that may be carbon sources for the fungus. Therefore, e.g., the feedstock comprising ca.89 wt% furfural, based on the total dry weight of the dissolved carbon sources, does only comprise added furfural and is free of added xylose.2Elapsed fermentation time (hours) denotes the elapsed time from the inoculum was aseptically inoculated into the production medium (feedstock) in the tank bioreactor to when a sample of suspension comprising formed fungal biomass was collected from the bioreactor (whereafter the suspension comprising formed fungal biomass was dewatered, and the crude protein content of the dewatered fungal biomass was analyzed using the Dumas method). An asterix (“*”) after the elapsed time denotes the elapsed time from the inoculum was aseptically inoculated into the production medium (feedstock) in the tank bioreactor to when the feedstock was changed to a feedstock comprising a higher concentration of furfural. E.g., at elapsed time 73:26 h a sample of suspension comprising formed fungal biomass was collected, whereafter the feedstock, which had been used so far (comprising 44.4 wt% furfural, based on the total dry weight of the one or more dissolved carbon sources) in the continuous preparation was changed to a feedstock comprising 50 wt% furfural, based on the total dry weight of the one or more dissolved carbon sources, and was used as production media (feedstock) in the continuous preparation. In table 4 is shown the fatty acid compositions of selected fatty acids of two fungal biomasses prepared by using a method of example 3, wherein the feedstocks used in the methods to prepare the fungal biomasses are according to table 2 and comprising xylose but not furfural or comprising furfural but not xylose. The formed fungal biomass prepared using a feedstock comprising added xylose (ca. 89 wt%, based on the total dry weight of the one or more dissolved carbon sources) but no added furfural has a crude protein content of 55.5 %, and the formed fungal biomass prepared using a feedstock comprising added furfural (ca. 89 wt%, based on the total dry weight of the one or more dissolved carbon sources) but not xylose has a crude protein content of 65.3 % (determined with the Dumas method). Using furfural in the feedstock in the continuous preparation of fungal biomass results in increased content of linoleic acid, based on the total weight of the one or more fatty acids of the fungal biomass. I.e., the (linoleic acid amount):(total fatty acid amount) ratio is increased by using furfural in the feedstock. The content of linoleic acid (C18:2 ω6) of the formed fungal biomass increased to 69.6 wt%, based on the total weight of the fatty acids of the fungal biomass, when using ca. 89 wt% furfural in the feedstock. The content of fatty acids was determined by using GC-FID (Gas Chromatography with Flame Ionization Detection). Table 4. Fatty acid compositions of selected fatty acids of two fungal biomasses prepared by using a method of example 3, wherein the feedstock comprises added xylose but no added furfural or comprises added furfural but no added xylose. 1Content (wt%) of the selected fatty acids are based on the total weight of the fatty acids of the fungal biomass. EXAMPLE 4: Feedstock comprising carbon sources of furfural, acetate, and formate, and different dilution rates in continuous preparations of fungal biomasses The method of example 2 was used and as the production medium (i.e., as feedstock and fresh feedstock) was used the one disclosed in table 2, except that instead of the xylose and furfural of the feedstock of table 2 the production medium comprised furfural (4.15 g / L), acetate (3.04 g / L), and formate (0.78 g / L). In this series of parallel continuous preparations of fungal biomasses dilution rates 0.1 h-1, and 0.2 h-1were used, and the crude protein contents (total dry weight basis) were determined using the Dumas method. Again, high crude protein contents of fungal biomasses were obtained (table 5). Increasing the dilution rate results in higher crude protein content of the fungal biomass (table 5). Table 5. Examples of obtained crude protein contents (%) of fungal biomasses obtained using feedstock and fresh feedstock comprising furfural, acetate, and formate, and different dilution rates (h-1) in continuous preparations of fungal biomasses. It is obvious to a person skilled in the art that with the advancement of technology, the inventive concept can be implemented in various ways. The invention and its embodiments are thus not limited to the examples described above; instead they may vary within the scope of the claims. The embodiments described hereinbefore may be used in any combination with each other. Several of the embodiments may be combined together to form a further embodiment. A product, a system, a method, or a use, disclosed herein, may comprise at least one of the embodiments described hereinbefore. It will be understood that the benefits and advantages described above may relate to one embodiment or may relate to several embodiments. The embodiments are not limited to those that solve any or all of the stated problems or those that have any or all of the stated benefits and advantages.

Claims

CLAIMS 1. A fungal biomass comprising one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae; and one or more fatty acids, wherein the fungal biomass has a crude protein content of at least 56 %, based on the total dry weight of the fungal biomass, and wherein the one or more fatty acids comprises one or more polyunsaturated fatty acids, wherein the content of the one or more polyunsaturated fatty acids is at least 56 wt%, based on the total weight of the one or more fatty acids, wherein the one or more filamentous fungi is each independently selected from the fungal genera Paecilomyces, Byssochlamys, Spicaria, Aspergillus, Penicillium, Rasamsonia, and Thermoascus.

2. The fungal biomass according to claim 1, wherein the one or more filamentous fungi is each independently selected from the fungal species Paecilomyces variotii, Paecilomyces puntonii, Byssochlamys nivea, Spicaria divaricata, Aspergillus niger, and Aspergillus oryzae.

3. The fungal biomass according to claim 1 or 2, wherein the one or more filamentous fungi is Paecilomyces variotii strain KCL-24.

4. The fungal biomass according to any one of the preceding claims, wherein one of the one or more polyunsaturated fatty acids is linoleic acid, wherein the content of linoleic acid is at least 56 wt%, based on the total weight of the one or more fatty acids.

5. A method for the continuous preparation of a fungal biomass, comprising: v) providing one or more filamentous fungi each independently selected from the fungal family Trichocomaceae, Thermoascaceae, and / or Aspergillaceae; vi) combining a feedstock comprising one or more dissolved carbon sources, wherein one of the one or more dissolved carbon sources is furfural, with the one or more filamentous fungi; vii) cultivating the combined one or more filamentous fungi and the feedstock under aerobic conditions to form the fungal biomass; andviii) collecting formed fungal biomass from the feedstock, wherein the dilution rate is 0.3 h-1or less, and wherein, in ii), the concentration of furfural in the feedstock is selected from 30 - 100 wt%, based on the total dry weight of the one or more dissolved carbon sources, wherein the one or more filamentous fungi is each independently selected from the fungal genera Paecilomyces, Byssochlamys, Spicaria, Aspergillus, Penicillium, Rasamsonia, Talaromyces, and Thermoascus.

6. The method according to claim 5, wherein, in iv), formed fungal biomass is collected from the feedstock when the formed fungal biomass has a crude protein content of at least 56 %, preferably 56 - 74 %, more preferably 61 –74 %, based on the total dry weight of the fungal biomass.

7. The method according to any one of claims 5 – 6, wherein the one or more filamentous fungi is each independently selected from the fungal species Paecilomyces variotii, Paecilomyces puntonii, Byssochlamys nivea, Spicaria divaricata, Aspergillus niger, and Aspergillus oryzae.

8. The method according to any one of claims 5 – 7, wherein the one or more filamentous fungi is Paecilomyces variotii strain KCL-24.

9. The method according to any one of claims 5 – 8, wherein, in ii), the one or more dissolved carbon sources content of the feedstock is 0.5 – 10 wt%, preferably 2 – 4 wt%, more preferably 2 –3 wt%.

10. The method according to any one of claims 5 – 9, wherein the dilution rate is 0.1 - 0.2 h-1.

11. An edible composition comprising fungal biomass as defined in any of claims 1 - 4.

12. Use of a fungal biomass as defined in any of claims 1 – 4 or an edible composition as defined in claim 11 as or in foodstuff.

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