Topical anti-aging cosmetic composition comprising flocculosin

Abstract

Anti-aging cosmetic composition for topical use comprising flocculosin as active ingredient, the remainder of the composition comprising compounds generally acceptable in the cosmetic field and more particularly between 0.05% and 6% by mass of a solution of 0.8 to 8 g per liter of flocculosin.

Description

Title of the invention: Topical anti-aging cosmetic composition comprising flocculosin FIELD OF THE INVENTION

[0001] The technical field of the present invention relates to a topical anti-aging cosmetic composition comprising flocculosin as active ingredient and an improved production process. STATE OF THE ART

[0002] Human skin is constantly exposed to environmental stress and undergoes changes that can accelerate its aging. Examples include scarring and skin aging related to the inflammation that is constantly triggered in the skin.

[0003] Thus, better healing of the skin and a reduction in its inflammation helps to combat skin aging. Man is therefore constantly looking for processes and active ingredients, particularly cosmetic ones, to accelerate healing and reduce the inflammatory reaction of the skin.

[0004] There is therefore a need for new active ingredients to combat the attacks suffered by human skin.

[0005] The fungus Pseudozyma flocculosa was discovered in 1988 on the surface of clover leaves. This fungus has the particularity of living epiphytically on the surface of certain plants such as clover while protecting this plant against the attack of pathogens by producing a molecule called flocculosin.

[0006] The molecular structure of flocculosin is as follows:

[0007] [Chem.l]

[0008] Flocculosin is a glycolipid of 854 Daltons and is mainly known today for its antibacterial properties. It allows certain plants to be naturally protected against the attack of pathogenic microorganisms.

[0009] Document WO2004007515 for example describes an antibacterial use of flocculosin and its derivatives.

[0010] Document WO20009129525 describes a use of flocculosine to combat dental caries.

[0011] To date, no study has demonstrated the beneficial effects of flocculosine in a cosmetic composition intended to combat skin aging. Statement of the invention

[0012] The invention therefore relates to an anti-aging cosmetic composition for topical use comprising flocculosine as active ingredient, the remainder of the composition comprising compounds generally acceptable in the cosmetic field.

[0013] Advantageously, the composition comprises between 0.05% and 6% by mass of a solution of 0.08 to 8 g per liter of flocculosine.

[0014] Advantageously, the composition comprises between 0.05% and 6% by mass of a solution of 0.5 grams per liter of flocculosine.

[0015] Advantageously, the composition also comprises at least one antioxidant compound.

[0016] According to one embodiment of the invention, the composition is in the form of a cream, a balm, a lotion, an oil, a butter or a spray.

[0017] According to another embodiment of the invention, the composition is in the form of a cream comprising: • 77.5% by mass of demineralized water, • 0.9% by mass of phenoxyethanol, • 3.0% by mass of propanediol, • 0.4% by mass of a mixture of xanthan gum and gum sclerotium rolfssii, • 4.0% by mass of a mixture of cetearyl olivate and sorbitan olivate, • 2.0% by mass of a mixture of cetyl palmitate, sorbitan palmitate and sorbitan olivate, • 7.0% by mass of coco-caprylate, • 0.2% by mass of ethylhexylglycerin, • 2.0% shea butter, • 1.0% by mass of passion fruit seed oil, and • 2.0% by mass of a 0.5 gram per liter solution of flocculosine.

[0018] According to yet another embodiment of the invention, the composition is in the form of a cream comprising: • 73.0% by mass of demineralized water, • 0.9% by mass of phenoxyethanol, • 0.3% by mass of a mixture of maltodextrin and biosaccharide gum-1, • 3.0% by mass of glycerin, • 0.3% by mass of a mixture of xanthan gum and gum sclerotium rolfssii, • 4.0% by mass of a mixture of polyglyceryl-6 distearate, esters of jojoba, cetyl aloccol and polyglyceryl-3 beeswax, • 0.3% by mass of glyceryl stearate citrate, • 3.0% by mass of vegetable oil, • 2.0 by mass of cetyl alcohol, • 4.0% by mass of caprylic / capric tryglyceride, • 4.0% by mass of octyldodecanol, • 0.2% by mass of ethylhexylglycerin, and • 5.0% by mass of a 0.5 gram per liter solution of flocculosine.

[0019] According to yet another embodiment of the invention, the composition is in the form of a butter comprising: • 3.0% by mass of polyglyceryl-3 diisostearate • 66.9% olive oil by mass, • 23.5% by mass of mango butter, • 5.9% by mass of a mixture of vitamin E, sunflower seed oil, sunflower wax and ascorbyl palmitate, • 0.2% by mass of linseed oil / palm oil aminopropanediol esters, • 0.2% by mass of flocculosin, and • 0.3% by mass of vitamin E.

[0020] According to yet another embodiment of the invention, the composition is in the form of a lotion: • 90.1% by mass of demineralized water, • 0.9% phenoxyethanol, • 3.0% by mass of a 0.5 gram per liter solution of flocculosine, • 1.0% by mass of propanediol, • 1.0 by mass of a mixture of caprylyl / capryl glucoside and water, • 1.0% by mass of a mixture of glycerin, sucrose laurate, sucrose dilaurate, sucrose trilaurate and sorbitol • 3.0% by mass of a mixture of 1,2-hexanediol, water and biosaccharide gum-4.

[0021] According to yet another embodiment of the invention, the composition is used as a healing or anti-inflammatory composition.

[0022] The invention also relates to a process for producing flocculosin comprising: • a first step of pre-cultivating the Pseudozyma flocculosa fungus in order to obtain a pre-culture, • a second stage of seeding by pre-cultivation of a culture medium comprising a carbon source so as to obtain a culture, • a third stage of fermentation of the culture in order to obtain a fermented culture, • a fourth step of lysis of the cells of the fermented culture in order to obtain a lysate, • a fifth step of solid-liquid separation of the lysate in order to obtain the flocculosin solubilized in a solvent, and • a sixth stage of filtration of solubilized flocculosin.

[0023] A very first advantage of the composition according to the invention lies in its excellent properties against skin aging.

[0024] Another advantage of the invention lies in the healing effect of the composition.

[0025] Another advantage of the present invention lies in the anti-inflammatory effect of the composition.

[0026] Yet another advantage of the present invention lies in the anti-aging effect of the composition.

[0027] An advantage of the present invention lies in the fact that it allows the industrial manufacture of flocculosine.

[0028] Another advantage of the present invention lies in the purity of the flocculosine obtained exceeding 80%. Brief description of the drawings

[0029] Other characteristics, advantages and details of the invention will be better understood on reading the additional description which follows in relation to the drawings in which:

[0030] [Fig-1] represents a graph illustrating the effect of flocculosin on macrophage-mediated inflammation, and

[0031] [Fig.2] represents a graph illustrating the effect of flocculosin on healing at the level of keratinocytes.

[0032] DETAILED DESCRIPTION OF EMBODIMENTS OF THE INVENTION

[0033] As described above, the invention relates to a cosmetic composition for topical application comprising flocculosine as active ingredient. This may be in the form of a solution based on propanediol or any other compatible solvent.

[0034] In the manufacture of cream, balm, gel or other products, it is preferred to use a solution preferably containing 0.5 grams per liter of flocculosine. The solution itself can be incorporated at a percentage by mass preferably varying between 0.05 and 6%.

[0035] The inventors have developed a process for producing flocculosin from the fungus Pseudozyma flocculosa and the use of this flocculosin as an active ingredient.

[0036] The method comprises a first step of precultivating the fungus Pseudozyma flocculosa. To do this, a cryotube stored at -80°C and comprising the fungus is thawed, then the fungus is inoculated onto a preculture medium. The preculture medium is an agar medium based on plant extract.

[0037] This step allows a scale-up on a liquid medium in order to reach a sufficient volume of fungus so as to obtain a preculture intended to inoculate another culture medium.

[0038] A second step is then carried out by inoculating the preculture obtained in the first step into a culture medium so as to obtain a culture.

[0039] The culture medium comprises a carbon source, for example glucose, to optimize the growth of the fungus.

[0040] The third step of the process consists of fermenting the culture so as to obtain a fermented culture. It is carried out aerobically and with stirring of the culture.

[0041] Following the fermentation of the culture, a fourth step of lysis of the cells of the fermented culture is carried out in order to obtain a lysate. This step is carried out when at least 85% of the carbon source of the culture medium is consumed by the fungus.

[0042] Cell lysis marks the end of the fermentation stage and is carried out by applying a heat treatment at 100°C to the fermented culture.

[0043] The lysate thus obtained comprises all the components derived from the fungus Pseudozyma flocculosa including flocculosin. The lysate is therefore subjected to a fifth step of solid-liquid separation so as to obtain the flocculosin solubilized in a solvent, for example propanediol.

[0044] Propanediol also allows good solubilization of flocculosin.

[0045] In the sixth step, the solubilized flocculosin is filtered to remove unwanted components. This step is carried out using a filtration membrane with a pore size of at most 22 μm.

[0046] At the end of the process, flocculosine is obtained on an industrial scale at a concentration of between 0.02% and 0.08% by mass in a solvent with a minimum purity of 80%.

[0047] The inventors subsequently studied and analyzed the properties of flocculosine and discovered by chance that this molecule had properties for cosmetic applications and more particularly anti-aging properties.

[0048] The inventors thus discovered that flocculosin possesses immunomodulatory and anti-inflammatory properties. Flocculosin also helps combat macrophage-mediated inflammation.

[0049] The inventors therefore conducted experiments to evaluate the effect of flocculosine on the release of reactive oxygen species (ROS) by macrophages.

[0050] To do this, monocytes were isolated from peripheral blood mononuclear cells (PBMCs) from blood samples of anonymized healthy volunteers. Isolation was performed by adherence to the plastic support for 2 hours in a Macrophage-SFM (M-SFM) cell medium optimized for macrophage culture, at 37°C and in a humidified atmosphere containing 5% CO2.

[0051] Monocytes are allowed to adhere to the plastic surface and undergo spontaneous and time-dependent differentiation into macrophages.

[0052] After isolation of the cells by adhesion, the macrophages were separated into three batches. The F-FLOCC product comprising 0.05% by mass of flocculosine in propanediol was introduced into the culture medium of the 3 batches for 24 hours at a respective concentration of 0.06%, 0.2% and 1% by mass.

[0053] Then, the culture media containing the different concentrations of the F-FLOCC product were removed and luminol was added to each culture medium (HBSS) to measure in real time the secretion of superoxide anion in the Luminoskan device. Phorbol myristate acetate (PMA) was then added at a concentration of 100 nM just before the measurement and the ROS released into the medium were measured continuously for 110 minutes.

[0054] For the analysis of the results, the areas under the curve (AUC) are then calculated (n=3 per condition).

[0055] [Fig. 1] represents a graph illustrating the results of the effect of flocculosin on macrophage-mediated inflammation by analyses on the release of reactive oxygen species by these macrophages.

[0056] The PMA showed, as expected, a strong pro-inflammatory capacity illustrated by the increase in the release of ROS by macrophages compared to the untreated control. This is reflected in the figure by an average area under the curve of 6642.7, reflecting the strong inflammation.

[0057] The F-FLOCC product showed a potent immunomodulatory and anti-inflammatory effect in dose-response demonstrated by the reduction of ROS release in inflammatory condition (+PMA) compared to the PMA-control condition.

[0058] Using a concentration of 0.06% of the industrial product, a reduction in inflammation of 23% is observed. The reduction is 38% at a concentration of 0.2% and 40% at a concentration of 1% of the F-FLOCC product.

[0059] We see that for the measurements at the three concentrations indicated above the average area under the curve is respectively 5314.8, 4428.4 and 4327.3.

[0060] p represents the standard deviation of all measurements taken. The value p<0.0001 reflects the reliability of the inflammation measurement at a concentration of 1.00%.

[0061] This result is particularly interesting for cutaneous applications of cosmetic compositions comprising flocculosine in the fight against aging.

[0062] The inventors also conducted experiments to evaluate the effect of flocculosin on the healing of keratinocytes after a standardized wound.

[0063] To do this, the inventors tested the effect of the F-FLOCC product comprising 0.05% by mass of flocculosine in propanediol on normal human epidermal keratinocytes (NHEKs) seeded in 96-well plates.

[0064] The wounds were made using the Woundmaker module (Sartorius), a device that produces standardized wounds simultaneously in each well.

[0065] Immediately after wounding, the F-FLOCC product is applied at different concentrations and culture plates are placed in the Incucyte S3® device for photographic acquisition with an interval of 6 hours for 3 days at 37°C and 5% CO2 in a humid atmosphere.

[0066] The F-FLOCC product is tested at a concentration of 0.2% and 0.1% by mass. The S-PROV solvent alone (propanediol) is also tested at a concentration of 0.2% by mass.

[0067] [Fig.2] illustrates the results of the effect of flocculosin on keratinocyte healing after a standardized wound and the measurement of wound closure.

[0068] It is noted that with propanediol (S-PROV) the wound is not closed after 3 days (-30% compared to the control).

[0069] Conversely, the F-FLOCC product significantly stimulated the migration of keratinocytes after the wound when compared to the control and the solvent. The F-FLOCC product at a concentration of 0.1% by mass strongly stimulates healing cutaneous. This [Fig.2] shows that with a concentration of 0.2 and 0.1%, we return to the level of the area under the curve of a closed wound.

[0070] The standard deviation values ​​p confirm the reliability of the measurements on wound closure.

[0071] Thus, the experiments carried out by the inventors on the effect of flocculosine against macrophage-mediated inflammation and on the healing of keratinocytes show the interest of this molecule in cosmetic applications, particularly in the field of anti-aging.

[0072] The concentrations of flocculosin used in these cell culture experiments represent between 1 and 50 times less than the dose to be used for topical applications with usual cosmetic compositions. This therefore corresponds to concentrations of use of flocculosin in a cosmetic composition of between 0.05% by mass and 6% by mass.

[0073] The inventors have therefore developed cosmetic compositions comprising between 0.05% and 6% by mass of a solution of 0.5 grams per liter of flocculosin. The cosmetic compositions according to the invention comprise, for example, 0.1%, 0.2%, 0.5%, 0.7%, 1%, 2%, 3%, 4% or 5% by mass of a solution of 0.5 grams per liter of flocculosin.

[0074] They carried out the same experiments as previously and found that cosmetic compositions comprising 0.05% and 6% by mass of a 0.5 gram per liter solution of flocculosine exhibited anti-inflammatory and healing properties on the skin.

[0075] The cosmetic composition according to the invention also comprises compounds generally acceptable in the cosmetic field.

[0076] Thus the composition according to the invention may comprise preservatives such as phenoxyethanol and ethylhexylglycerin, alone or as a mixture.

[0077] The composition according to the invention may also comprise moisturizing agents such as glycerin and propanediol, alone or in a mixture, the biosaccharide gum-1.

[0078] The composition according to the invention may also comprise gelling agents such as Sclerotium rolfssii gum and xanthan gum, alone or as a mixture.

[0079] The composition according to the invention may also comprise emulsifying agents such as cetearyl olivate, sorbitan olivate, polyglyceryl-6 distearate, jojoba esters, polyglyceryl-3 beeswax, cetyl alcohol, glyceryl stearate citrate, polyglyceryl-3 diiostearate, glycerin, sorbitan palmitate, sorbitan olivate, sorbitol, sucrose laurate, sucrose dilaurate and sucrose trilaurate, alone or in mixtures.

[0080] The composition according to the invention may also comprise emollient agents such as sunflower wax, sunflower seed oil, caprylic / capric triglyceride, vegetable oil, passion fruit seed oil, coco-caprylate and octyldodecanol, alone or in mixture.

[0081] The composition according to the invention may also comprise antioxidant agents such as vitamin E, ascorbyl palmitate, alone or as a mixture.

[0082] The compositions according to the invention may also comprise butters such as shea butter and mango butter, alone or as a mixture.

[0083] The composition according to the invention may also comprise vegetable oils such as olive oil and passion fruit oil, alone or in mixtures.

[0084] The composition according to the invention may also comprise the active ingredient known under the trade name Fucogel®Powder (INCI name: maltodextrin & biosaccharide gum-1). It is a high molecular weight anionic polysaccharide (1.106 Da) obtained by bacterial fermentation from plant substrates. It is composed of L-fucose, D-galactose and galacturonic acid and is in the form of a powder on a maltodextrin support. It has soothing, moisturizing, anti-aging, restructuring properties and is also used as a touch agent. Fucogel®Powder derives its biological effectiveness from its affinity for cutaneous fucose receptors but also from its unique ability to modulate neuronal sensitivity via the ASIC receptor pathway.

[0085] The composition according to the invention may also comprise an active ingredient known under the trade name Omega 3 Ceramide® Lin (INCI name: linseed oil / palm oil aminopropanediol esters). It is a bioceramide obtained from a vegetable oil rich in linolenic acid. It allows the stabilization of unsaturated fatty acids to facilitate their dissolution. It also allows the formation of a biomimetic ceramide structure intended to strengthen or restore the intercorneocyte lipid cement, for better skin restructuring and protection. It also allows the vectorization of unsaturated fatty acids and / or other biological molecules to maintain and optimize the biological properties of the grafted active molecules.

[0086] The composition according to the invention may also comprise the active ingredient known under the trade name Pollustop® (INCI name: Biosaccharide gum-4 (and) 1,2-hexanediol). It is a high molecular weight anionic polysaccharide obtained by biotechnology. This active ingredient is generally used for daily protection of the skin and hair, as an anti-pollution, anti-aging and anti-UV agent.

[0087] The following description relates to examples of compositions according to the invention.

[0088] Example 1: Anti-aging day cream

[0089] [Tables 1] Product INCI Name Mass Percentage Demineralized Water AQUA (WATER) 77.5 PHENOXYETHANOL PHENOXYETHANOL 0.9 1.3 PROPANEDIOL CO SMOS PROPANEDIOL 3.0 ACTIGUM™ VSX20 SCLEROTIUM GUM XANTHAN GUM 0.4 OLIVEM 1000 CETEARYL OLIVATE SORBITAN OLIVATE 4.0 OLIWAX® LC CETYL PALMITATE SORBITAN PALMITATE SORBITAN OLIVATE 2.0 CETIOL® C5 COCO-CAPRYLATE 7.0 MICROCARE® EHG ETHYLHEXYLGLYCERIN 0.2 LIPEX SHEA™ BUTYROSPERMUM PARKII (SHEA) BUTTER 2.0 PASSION FRUIT OIL PASSIFLORA EDULIS SEED OIL 1.0 FLOCCULOSIN solution at 0.5 grams per liter of flocculosin 2.0

[0090] Example 2: Anti-aging night cream

[0091] [Tables2] Product INCI name Mass percentage Demineralized water AQUA (WATER) 73.0 PHENOXYETHANOL PHENOXYETHANOL 0.9 FUCOGEL® POWDER MALTODEXTRIN BIOSACCHARIDE GUM-1 0.3 GLYCERIN GLYCERIN 3.0 ACTIGUM™ VSX20 SCLEROTIUM GUM XANTHAN GUM 0.3 EMULIUM® MELLIF ERA POLYGLYCERYL-6 DISTEARATE JOJOBA ESTERS POLYGLYCERYL-3 BEESWAX CETYL ALCOHOL 4.0 AXOL® C 62 PELLETS GLYCERYL STEARATE CITRATE 0.3 SonneNATURAL® OLUS OIL (VEGETABLE OIL) 3.0 NACOL 16-98 CETYL ALCOHOL 2.0 DUB™ MCT 5545 CAPRYLIC / CAPRIC TRIGLYCE RIDE 4.0 ISOFOL 20 OCTYLDODECANOL 4.0 MICROCARE® EHG ETHYLHEXYLGLYCERIN 0.2 FLOCCULOSIN solution at 0.5 grams per 1 liter of flocculosin 5.0

[0092] Example 3: Regenerating body butter

[0093] [Tables3] Product INCI Name Percentage by mass CITHROL+TM PG32IS- LQ-(MV) POLYGLYCERYL-3 DIISOSTEARATE 3.0 REFINED OLIVE OIL OLEA EUROPAEA (OLIVE) FRUIT OIL 66.9 MANGO BUTTER MANGIFERA INDICA (MANGO) SEED BUTTER 23.5 KAHLWAX™ 6607L MB SUNFLOWER HELIANTHUS ANNUUS (SUNFLOWER) SEED WAX ASCORBYL PALMITATE TOCOPHEROL HELIANTHUS ANNUUS (SUNFLOWER) SEED OIL 5.9 Omega 3 CERAMIDE® LIN LINSEED OIL / PALM OIL AMINOPHENIDIOL ESTERS 0.2 FLOCCULOSIN solution at 0.5 grams per 1 liter of flocculosin 0.2 COVI-OX® T-70 EU TOCOPHEROL 0.3

[0094] Example 4: Anti-pollution and anti-aging micellar lotion

[0095] [Tables4] Product INCI Name Mass percentage Demineralized water AQUA (WATER) 90.1 PHENOXYETHANOL PHENOXYETHANOL 0.9 FLOCCULOSINE solution at 0.5 grams per 1 liter of flocculosine 3.0 1.3 PROPANEDIOL CO SMOS PROPANEDIOL 1.0 ORAMIX™ CG 110 CAPRYLYL / CAPRYL GLUCOSIDE AQUA (WATER) 1.0 NATPURE® SOL GLYCERIN SUCROSE LAURATE SUCROSE DILAURATE SUCROSE TRILAURATE SORBITOL 1.0 POLLUSTOP® AQUA (WATER) 1,2-HEXANEDIOL BIOSACCHARIDE GUM-4 3.0

[0096] To enable application of the composition according to the invention to all types of skin, wounds and / or inflamed skin areas, the composition according to the invention may be in the form of a cream, a balm, a lotion, an oil, a butter or a spray.

Claims

Claims

1. Composition in a form suitable for topical application comprising flocculosine as active ingredient, for use in combating macrophage-mediated inflammation and / or in promoting healing mediated by keratinocyte migration.

2. Composition according to the preceding claim, characterized in that it comprises between 0.05% and 6% by mass of a solution of 0.08 to 8 g per liter of flocculosin.

3. Composition according to claim 2, characterized in that it comprises between 0.05% and 6% by mass of a solution of 0.5 g per liter of flocculosin.

4. Composition according to any one of the preceding claims, characterized in that it is in the form of a cream, a balm, a lotion, an oil, a butter or a spray.

5. Composition according to claim 1, characterized in that it is in the form of a cream comprising: • 77.5% by mass of demineralized water, • 0.9% by mass of phenoxyethanol, • 3.0% by mass of propanediol, • 0.4% by mass of a mixture of xanthan gum and Sclerotium rolfssii gum, • 4.0% by mass of a mixture of cetearyl olivate and sorbitan olivate, • 2.0% by mass of a mixture of cetyl palmitate, sorbitan palmitate and sorbitan olivate, • 7.0% by mass of coco-caprylate, • 0.2% by mass of ethylhexylglycerin, • 2.0% of shea butter, • 1.0% by mass of passion fruit seed oil, and • 2.0% by mass of mass of a 0.5 gram per liter solution of flocculosin.

6. Composition according to claim 1, characterized in that it is in the form of a cream comprising: • 73.0% by mass of demineralized water, • 0.9% by mass of phenoxyethanol, • 0.3% by mass of a mixture of maltodextrin and biosaccharide gum-1, • 3.0% by mass of glycerin, • 0.3% by mass of a mixture of xanthan gum and sclerotium rolfssii gum, • 4.0% by mass of a mixture of polyglyceryl-6 distearate, jojoba esters, cetyl alcohol and polyglyceryl-3 beeswax, • 0.3% by mass of glyceryl stearate citrate, • 3.0% by mass of vegetable oil, • 2.0% by mass of cetyl alcohol, • 4.0% by mass of caprylic / capric triglyceride, • 4.0% by mass of octyldodecanol, • 0.2% by mass of ethylhexylglycerin, and • 5.0% by mass of a 0.5 gram per liter solution of flocculosine.

7. Composition according to claim 1, characterized in that it is in the form of a butter comprising: • 3.0% by mass of polyglyceryl-3 diisostearate • 66.9% by mass of olive oil, • 23.5% by mass of mango butter, • 5.9% by mass of a mixture of vitamin E, sunflower seed oil, sunflower wax and ascorbyl palmitate, • 0.2% by mass of linseed oil / palm oil aminopropanediol esters, • 0.2% by mass of a solution of 0.5 grams per liter of flocculosine, and • 0.3% by mass of vitamin E.

8. Composition according to claim 1, characterized in that it is in the form of a lotion: 90.1% by mass of demineralized water, 0.9% phenoxyethanol, 3.0% by mass of a 0.5 gram per liter solution of flocculosin, 1.0% by mass of propanediol, 1.0 by mass of a mixture of caprylyl / capryl glucoside and water, 1.0% by mass of a mixture of glycerin, sucrose laurate, sucrose dilaurate, sucrose trilaurate and sorbitol 3.0% by mass of a mixture of 1,2-hexanediol, water and biosaccharide gum-4.

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