Systems for the collection and intraoperative analysis of biological tissues

Abstract

The invention relates to a system for sampling biological tissues from an area of ​​interest, receiving a biomarker prior to or simultaneously with the surgical procedure. The system comprises an excision head connected to a tissue receptacle (300) by a suction transfer tube. The system is characterized in that it comprises: a detection sleeve surrounding a reference zone of said suction transfer tube; said detection sleeve comprising at least one sensor sensitive to said biomarker; and an electronic circuit delivering an electrical signal representative of the presence or absence of the marker when a sampled tissue passes through said reference zone. Abstract figure: Figure 1

Description

Title of the invention: Systems for the intraoperative collection and analysis of biological tissues. Field of invention

[0001] The invention relates to surgical equipment intended for the sampling of all biological tissues, in particular tumors, by a detection and / or excision head connected to a suction tube ensuring the transfer of the tissues sampled to a container receiving the tissues sampled for the purpose of real-time analysis during movement in the suction tube and / or subsequent anatomopathological diagnosis, including proteomic, metabolomic or genotypic studies.

[0002] In order to facilitate the localization of the tissues to be detected and analyzed, a product is injected intraoperatively either in the vicinity of the areas of interest, or intravascularly or lymphatically. Three products are most often used: a dye (injected in the operating room a few minutes before the surgical intervention), a fluorescent molecule or a radioactive product (injected a few hours before the surgical intervention or intraoperatively). These products are sometimes combined.

[0003] The sampled tissues are detected, counted and geolocated in the tumor region, then stored in identified and labeled containers. The sampled tissues are then either analyzed in the operating room or sent to the laboratory to be analyzed extemporaneously by anatomopathological analysis or possibly by biochemical and genotypic tests.

[0004] These techniques can also be used to improve the accuracy of tumor diagnosis of biopsies by guiding the surgeon to relevant tissue regions to determine the histological nature of the tumor.

[0005] These techniques make it possible to secure the surgical procedure by indicating to the surgeon in real time whether or not the tissue samples are tumorous. State of the art

[0006] Patent FR2908283B1 is known in the state of the art, which relates to a device provided with a part insertable into a tubular cavity comprising: a miniaturized probe for detecting gamma radiation comprising a part insertable into a tubular cavity which comprises at least: a scintillator crystal which emits light in response to ionizing radiation, preferably gamma radiation, and which has a volume of less than 30 mm3, the maximum cross-section of the probe being less than 10 mm2, an optical fiber ensuring the collection of at least part of the light emitted by the scintillator material, and its transmission to a photoelectric converter, a photoelectric converter to which the crystal is connected via the optical fiber which ensures the collection and transmission of at least part of the light emitted by the scintillator crystal to the photoelectric converter, and optical means for viewing the cavity, insertable into the latter.

[0007] Document US 5,651,783 describes a fiber optic assembly for receiving a surgical instrument, such as an intraocular surgery instrument. The assembly comprises a sleeve in the body of which extends a bundle of optical fibers, and connection means for connecting the bundle of optical fibers contained in the sleeve to an external optical cable connected to a light source.

[0008] Patent EP1933747Bl is also known, the aim of which is to enable the surgeon to perform an ablation with greater precision and speed than with prior art devices using a per-operative probe to guide an excision tool, comprising a detection head, said detection head comprising: • at least one optical fiber capable of receiving and guiding a signal emitted by a tissue area to analysis equipment, • fixing means for fixing the head on the excision tool, so that the excision tool is able to extract a portion of tissue in the area of ​​tissue emitting the signal.

[0009] The detection head is adapted to be coupled to an excision tool so that the surgeon can perform the tumor detection and ablation operations in a single movement, without moving the probe. This provides a more precise location of the tumor tissues since correlation errors between the position of the tumor obtained from the signal measured by the probe and its actual position in the surgical wound are eliminated. The possibility of simultaneously measuring the concentration of radioactive tracers and fluorescent molecules also makes it possible to benefit from the complementarity of the information by these two methods and therefore to reinforce the specificity of tumor detection. Disadvantage of the prior art

[0010] The solutions of the prior art are not entirely satisfactory because the collection receptacle contains, after the intervention, a mixture of all the tissues removed, including healthy tissues which complicate the subsequent analysis work and prevent concentration on the study of pathological tissues. Furthermore, the management of radioactive waste by hospitals is highly regulated. Indeed, certain pathologies, such as neurological pathologies, carry a risk and require that all waste used for treatment be destroyed: the waste is then traced, stored and then sent to an identified waste area. The mixture of marked and unmarked tissues increases the volume of special waste requiring costly treatment for its disposal.

[0011] The solutions of the prior art make it possible to quantitatively control in real time the relevance of the tumor excision of tissues detected by the fluorescent or radioactive marker but not to correlate counting data with other information for more sensitive detection of the tracers by isolating the detection of the “noise” of the surgical wound.

[0012] In order to overcome these drawbacks, the invention relates to a system for sampling biological tissues in an area of ​​interest receiving a biomarker prior to or simultaneously with the surgical intervention, said system comprising an excision head connected to a receptacle for the sampled tissues by a suction transfer pipe, characterized in that it comprises: - a detection sleeve surrounding a reference area of ​​said suction transfer pipe, said detection sleeve comprising at least one sensor sensitive to said biomarker - an electronic circuit delivering an electrical signal representative of the presence or absence of marker when a tissue sample passes through said reference zone. According to variants: - said electrical signal controls the transfer of the sampled tissue having just passed through said reference zone to said collection receptacle in the event of detection of a marker or to another receptacle by default - the system comprises a means for transiently actuating the suction delivering a time-stamped electrical signal, and in that said signal representative of the presence of a marker is also time-stamped - said sensor is formed by an annular organic scintillator which detects beta particles and gamma rays associated with a photodetector - said annular organic scintillator is surrounded by an inorganic gamma scintillator, also annular, which detects only gamma rays, said inorganic gamma scintillator being associated with a second photodetector - the system includes a valve directing the collected tissues to a collection receptacle or to a waste receptacle depending on the information provided by the detection sleeve - said receptacle is constituted by a barrel comprising a plurality of collection units - the system includes a pressure switch detecting the activation of the sample by the excision head - The system comprises an enclosure whose walls are lined with lead sheets, for the confinement of said receptacle - said enclosure comprises a plurality of housings each comprising a

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[0020] radiation sensor - said enclosure further comprises a housing for receiving a urine bag - the system includes said electronic circuit which controls a safety alert in the event of non-detection of biomarker - The system also includes a means of real-time analysis of tissues on certain histological and biochemical aspects Detailed description of a non-limiting example of embodiment The present invention will be better understood on reading the following description, concerning a non-limiting example of embodiment illustrated by the appended drawings where: [Fig.l] [Fig.l] represents a schematic view of a sampling system according to the invention [Fig.2] [Fig.2] represents a schematic view of an alternative embodiment of the sampling system according to the invention [Fig.3] [Fig.3] represents a schematic view in longitudinal section of a detection sleeve for a sampling system according to the invention [Fig.4] [Fig.4] represents a schematic cross-sectional view of a detection sleeve for a sampling system according to the invention General principle of the invention The system according to the invention consists of: - An excision tool (100) - A suction hose (200) preferably for single use - A set of receptacles (300) - A vacuum source (400) possibly connected by a connecting tube (325) to the hospital vacuum source - A computer terminal (500) - A detection sleeve (600) surrounding the suction pipe (200) The following description specifies the characteristics of the exemplary embodiment of some of the components of the system. It is specified that each of the components can be developed and used independently of the others and that it should not be considered that they are necessarily all combined with each other. The general principle of the invention consists in providing on the suction tube (200) connecting the excision head (100) to the receptacles (300) of the aspirated products one or more detectors intended to characterize in real time, during suction, in the suction pipe (200), the presence and / or the nature of the sample taken passing through the pipe, in order to control one or more functions such as the routing towards a particular receptacle of the assembly (300) or the emission of an audible or luminous signal in the event of excessive suction of unmarked samples. Excision tool and detection head

[0021] The invention is not limited to a particular type of excision tool, nor to a single gentle suction solution, but can be implemented with any type of excision or gentle suction tool passing the samples through a pipe.

[0022] According to an alternative embodiment, the excision tool (100) consists of a head (110) (preferably single-use) formed by a tubular element crossed by a suction channel having several possible geometries, modifiable depending on the operating cavity, the sensitivity or the resolution required.

[0023] This head (110) is completed by a photo-detection system (120), itself coupled to a computer terminal (500) controlled by data control, acquisition and visualization software.

[0024] The detection head (110) makes it possible to measure the particle concentration [3 in the tissues in the vicinity of the suction mouth. The signal thus received is transformed by the photodetection system into an electrical signal; this electrical signal is transmitted to the data acquisition module. The data are acquired, processed and displayed using computer software on a terminal (500).

[0025] The detection head (120) is composed in a known manner of an assembly of detection elements organized circularly around an excision tool (110). The scintillation light from the fiber detection elements is directly collected and detected by a photodetection system (detection ring), consisting of silicon photomultipliers. The electrical signals produced by each SiPM are routed to an electronic acquisition system which quantifies the spectrum and counting rate of the beta particles detected on each fiber element.

[0026] Each of the head's detection elements is composed of two parts: a clear optical fiber, topped with a scintillating optical fiber. The scintillating optical fiber is used to detect radioactive emissions (y or |3). When a y or [3 particle interacts with the scintillating optical fiber, a shower of scintillation photos is created. The clear optical fiber then serves as a light guide, directing the scintillation photons away from the surgical wound.

[0027] After its journey through the tissues, a [3+] particle can annihilate upon encountering an electron. Such annihilation produces two 511keV y photons which, if detected, disrupt the direct measurement of beta particles. Indeed, due to their large average path, the detected photons may come from non-specific accumulation zones of the radiotracer s, located at a distance from the region of interest. It is therefore advisable to reject this background noise to ensure the reliability of the measurement.

[0028] To do this, the flux of photons reaching the probe is evaluated by placing detection elements sensitive only to y radiation. These elements of detection are optical fibers covered with stainless steel shielding, called "witness" fibers, as opposed to unshielded fibers, called "signal" fibers ([Fig.l]). The signal from the witness fibers is subtracted from that from the signal fibers, to keep only the pure signal [3.

[0029] The thickness of the scintillating parts of the "signal" fibers is optimized to achieve the best compromise in terms of maximum positron energy deposition and minimal gamma background noise contamination. The length of the scintillating part of the "control" fibers is defined so as to reduce statistical fluctuations in the measurement of the gamma signal while minimizing the dependence of the subtraction method on spatial variations in the background noise.

[0030] To optimize the collection of light by each detection element, it was chosen to add an optical coating on each of the fibers, covering them with a thin layer of reflective paint which back-guides, through the clear fiber, the scintillation photons emitted in the opposite direction. This coating improves the sensitivity of the probe.

[0031] The choice of using optical fibers as the detection element for the probe is explained by the fact that they are both suitable for the detection of positrons, while being relatively insensitive to background noise.

[0032] Furthermore, the fibers can have various shapes and sizes, allowing flexibility in designing the geometry of the detection head. In this way, depending on the clinical context, the pathology being treated, the shape of the operating cavity, the chosen excision tool, etc., several head shapes can be proposed.

[0033] From a clinical point of view, optical fibers also guarantee the safety of the patient and the surgeon: they are electrically inert, which allows their introduction into the surgical wound and the coupling of the probe with an excision tool. Finally, they are compact, which allows the design of a miniaturized probe.

[0034] The choice of a single-use detection head simplifies post-operative sterilization of the tool, inherent in the practice of surgery, and allows the device to maintain good sensitivity. Indeed, an alternative approach would consist of protecting the probe with a plastic cover. However, the addition of such a cover would reduce the performance of the detection head.

[0035] The detection head is composed of plastic optical fibers and a metal part. The metal part could a priori be recovered and reused for the same purpose. Since the optical fibers are made of plastic, a study could be conducted on the advisability of recycling them.

[0036] The principle of using such an excision tool is as follows: after removing the After removing the main visible part of the tumor, which has been previously radiolabeled, the surgeon inserts the probe into the surgical wound. He first removes the visible part of the tumor using an excision tool, for example an ultrasonic aspirator.

[0037] A B-emitting radiotracer is then injected intravenously during the operation, intravenously, 30 to 60 minutes before checking the tumor margins in order to minimize radioactive contact with operating room staff.

[0038] The concentration of B particles is then measured using the detection head to locate the tumor residues and proceed to their removal and aspiration.

[0039] Local measurement of the concentration of the radioactive tracer around the excision tool makes it possible to locate any tumor residues. The detector probe is capable of accelerating the process of searching for cancerous tissues and of improving the quality of the surgical procedure by allowing the surgeon to define the margins of the tumor resection more precisely and in real time, which offers improved comfort and safety.

[0040] Surgeons can therefore remove tumor tissue more precisely while preserving the surrounding healthy tissue, allowing for greater preservation of the affected organs. This surgery, which is less heavy and more conservative, allows the patient to recover more quickly and maintain a better quality of life, by limiting the number of recurrences.

[0041] When using a detection head (120), the surgeon can choose between two viewing modes to receive real-time information on the location of the tumor tissues: a “counter” mode and an “imaging” mode. The counter mode provides the counting rate [3 in all the optical fibers of which the detection head is composed. The digital value is displayed on the screen of the terminal (500) and is supplemented by an audible signal having a frequency proportional to the counting rate, which allows the exploration to be carried out without taking one's eyes off the surgical wound. In imaging mode, each optical fiber is visually represented on the screen; the counting rate for each of the fibers is specified, accompanied by a color whose intensity is proportional to the indicated value. This image can be presented on a dedicated screen or directly on the microscope used by the surgeon, for example.

[0042] Online characterization of sampled tissues passing through the reference zone

[0043] The invention differs from the state of the art more particularly by the detection in the flow passing through the suction tube (200) of the passage of marked samples before transfer into a receptacle (300). This detection is carried out by a detection sleeve (600) arranged at a reference zone of the suction tube, at a known distance from the outlet in the receptacle (300).

[0044] The characterization of the tissues, and in particular the presence or absence of a marker, is carried out by a sleeve (600) comprising one or more sensors adapted to the traceability mode provided for the excision system.

[0045] This detection sleeve (600) passing through the suction tube (200) is arranged on a reference zone of the tube (200), determined by a distance Di from the downstream end of the tube (200) and a distance D2 from the upstream end of the tube (200). The detection can be carried out by simple optical barriers or those equipped with cameras, or by radiation or particle counters.

[0046] Knowledge of the distance Di and the average speed of movement of the samples in the suction tube makes it possible to determine the time lag of the passage in a direction valve (230) directing the marked samples towards a first receptacle (310) via a branch (315), or towards a waste receptacle (320) via another branch (325).

[0047] The two receptacles are connected to the vacuum source (400).

[0048] The information coming from the sleeve (600) makes it possible to control the valve (230) depending on whether or not a marker is detected. Data processing.

[0049] The sleeve (600) comprises one or more sensors for detecting the passage of a material, or for detecting the passage of a marked sample. These sensors comprise a microcontroller for exploiting the electrical signals generated by the detection. These signals are then subject to processing comprising all or part of the following functions: • Sampling and encoding signals to provide a digital data frame • Timestamp of the data frame or data • Data filtering to transmit only data of interest • Adding an incremental identifier to the data

[0050] This data is then used by an application by the terminal (500) to provide functionalities such as: • The routing of the sample towards one of the receptacles (300) depending on the characterization of the sample considered, • Counting the number of marked samples • Correlation with other information such as suction pressure or suction activation, or the image acquired by the detection head with a time lag where appropriate • Activation of an audible or light signal. Receptacles

[0051] Alternatively, the receptacle assembly (300) comprises a barrel formed of several receptacles moved after each transfer of a sample. The information provided by the sleeve (600) is in this case time-stamped and recorded in a table in relation to the receptacle into which the sample, characterized during passage in the reference zone, was transferred.

[0052] According to one variant, the receptacles comprise a PBS precision scale providing real-time information on the mass of samples collected.

[0053] The receptacles can be equipped with individual radioactivity sensors.

[0054] They may also be refrigerated to improve the preservation of the sampled tissues or contain a solution for fixing the sampled tissues such as formaldehyde. Radiation protection of the receptacle assembly

[0055] According to a preferred embodiment, the receptacles (310, 320) are enclosed in a confined radiation protection enclosure. This confined enclosure advantageously has a space for housing the patient's urinary catheter (450), and thus confines all of the patient's radiation sources in a radiation protection enclosure (800).

[0056] The enclosure is constituted by a box whose walls comprise lead sheets, typically sheets with a total thickness suitable for the absorption of gamma radiation with an energy lower than 1 MeV, intended to confine the gamma radiation emitted by the samples as well as by the patient's urine. The box has a housing for receiving a urine bag, as well as a housing for receiving the set of sampling receptacles (300). Passages are provided for the passage of the conduits of the urine bag and the tube (200) for transferring the samples, as well as the tube for connection to the hospital's vacuum source. Alternatively, certain passages are replaced by fluid connectors.

[0057] The radiation-safe receptacle (310) will preferably have separate compartments each equipped with a radiation sensor (360) to receive on the one hand the selected samples, the rejected samples and, if applicable, containers of patient fluids. The receptacle will preferably accommodate the selected samples, the collection of rejected tissue (with a specific sensor) and the urine bag with a sensor (350) and a volume indication (the urine bag will not be visible through the shielded confinement and it is important for the surgeon and the anesthesiologist to know the volume, the color (presence of blood for example), concentration, etc.). Sensors

[0058] Different types of sensors can be used for the characterization of the sample, in particular a light barrier formed by a light source oriented transversely relative to the axis of the tube (200) associated with a photodetector or a strip of photodetectors or a camera for counting the samples and possibly for imaging the section of the sample and / or a photodetector detecting the luminescence of a sample marked by a phosphorescent marker, or a photodetector associated with an excitation source detecting the luminescence of a sample marked by a fluorescent marker, or a detector capable of counting the radioactivity of a sample marked by a radioactive tracer.

[0059] The valve (230) can be controlled automatically from the signals provided by the sleeve (600), or manually by the surgeon; in the latter case, the sleeve (600) controls the activation of an audible and / or luminous signal upon detection of a marked sample, and the surgeon decides upon perception of this signal whether the sample in question should be kept or rejected, possibly taking into account other criteria originating from the intervention in progress.

[0060] The sleeve (600) can also be connected to a mass spectrometer to characterize the presence or absence of certain proteins or exogenous tracers (such as glucose, dopamine, etc.) or even a biochemical signature of a substance of interest. Radioactivity-sensitive sensor

[0061] According to a preferred embodiment, a sensor counts the radioactivity of the aspirated samples. For this purpose, the reference zone of the sleeve (600) comprises an interior tubular segment (610) consisting of a scintillator dedicated to the detection of beta particles. This interior tubular segment (610) is surrounded by photodetectors (630) wired on a printed circuit (620) and covered with a reflective coating (615) (except on the zones where the photodetectors are coupled).

[0062] This first scintillator (610) is surrounded by a second tubular segment (640), coaxial with the first, and consisting of a scintillator dedicated to the detection of gamma radiation. It is covered by a reflective coating (645) and equipped with photodetectors (660) wired on a printed circuit.

[0063] The detection zone is thus made up of two concentric scintillators (610, 640). Photodetectors (of the Silicon PhotoMultiplier or SiPM type) are coupled to the scintillators, on their external face and / or at their end.

[0064] The internal scintillator, called "beta scintillator" (610) is an annular organic scintillator which detects beta particles and gamma radiation. It is cylindrical on its internal face and faceted on its external face for optimal contact with the silicon photomultipliers (630) responsible for collecting the signal.

[0065] The external scintillator, called “gamma scintillator” (640), is an internal scintillator organic also annular, denser than the internal scintillator, (610) which is superimposed on it and which only detects gamma rays - the low average path of the beta particles emitted by the sample does not allow them to reach the gamma scintillator (640).

[0066] Using the data collected by the two scintillators (610, 640) via the photodetectors (630, 660), it is possible to deduce the beta or gamma counting rate of the sample proportional to the amount of radioactive tracer present in the sample, using a coincidence method.

[0067] The system thus makes it possible to measure:

[0068] - The beta count rate (with gamma signal correction);

[0069] - The gamma count rate alone.

[0070] A visual or audible safety alert is triggered when a sample passes through the detection sleeve without causing beta particle detection. In this case, the sample was taken from an unmarked and therefore non-cancerous area, and the operator is warned by the alert so that he can avoid continuing unnecessary sampling. Additional sensors

[0071] [Fig. 2] represents an exemplary embodiment comprising additional sensors.

[0072] A light barrier (390) formed by a light source arranged to form a beam transversely passing through the pipe (200) and a photodetector delivers a signal varying when a sample passes through the field of the beam of the light barrier (390). A microcontroller (380) performs processing of the electrical signal delivered by the light barrier (390) to deliver time-stamped information of the passage of the sample, or with an identifier correlated with an identifier or a time-stamp of the image of the sampling area acquired with the excision tool.

[0073] A sensor (370) detects the passage of the sample out of the sleeve (600) and provides a time stamp of this passage. Activation of the sensor can, depending on the detection result delivered by the sleeve (600), trigger a safety alert (sound or light signal) and / or control the valve (230). The two detection zones (390, 600) are separated by a distance D2 and measuring the time elapsed between the two makes it possible to determine the speed of movement of the sample in the pipe (200) and to characterize any anomalies by comparison with the nominal speed of movement.

[0074] Another light barrier (230) also provides information on the time elapsed between the passage of the sample in the sleeve and the travel of the pipe section of length Di and provides an alert in the event of drift compared to a reference duration.

[0075] The installation can further be equipped with an additional analysis means (700) connected to the detection sleeve (600), for example a mass spectrometer, or a fluorescence counter.

[0076] Sequencing of samples according to a non-limiting example of embodiment

[0077] The surgeon controls the aspiration of the tissues: the head of the excision tool in contact with the patient's tissues aspirates the tissue samples which are then drawn through the tube (200) to the receptacle assembly (300).

[0078] The samples are drawn by this suction through the tube (200) and pass into the reference zone where the detection sleeve checks the presence or absence of markers in the sample passing through it at a time t, which allows it to be directed towards a waste receptacle or a subsequent analysis receptacle.

[0079] According to a particular embodiment, a pressure switch type sensor detects the closing of the hole and therefore the start of a sampling sequence, and the state of this sensor is time-stamped and recorded, as well as possibly the image of the sampling zone in the operating wound provided by the detection head (120), which is also recorded in time-stamped form.

[0080] The signals provided by the sleeve (600) are also recorded in a time-stamped manner.

[0081] The resynchronization of the information takes into account the average times traveled by a sample in the tube (200) in the length D2 between the excision tool and the sleeve (600) and in the length Di between the receptacle (300) and the sleeve (600).

[0082] This information makes it possible to provide additional information for the tissue sampled and collected at a given time. By providing a barrel with a plurality of receptacles, each sequence of tissue sampled is collected in a specific receptacle, associated with a reference time possibly making it possible to associate with it the image provided by the imager of the excision tool as well as the information provided by the sleeve (600).

Claims

Claims

1. System for sampling biological tissues in an area of ​​interest receiving a biomarker prior to or simultaneously with the surgical intervention, said system comprising an excision head (100) connected to a receptacle (300) for the tissues sampled by a suction transfer pipe (200), characterized in that it comprises: - a detection sleeve (600) surrounding a reference area of ​​said suction transfer pipe (200), said detection sleeve (600) comprising at least one sensor sensitive to said biomarker - an electronic circuit delivering an electrical signal representative of the presence or absence of marker during the passage of a tissue sampled in said reference area.

2. System for sampling biological tissues according to claim 1 characterized in that said electrical signal controls the transfer of the sampled tissue having just passed through said reference zone to said collection receptacle in the event of detection of a marker or to another receptacle by default.

3. System for sampling biological tissues according to claim 1, characterized in that it comprises a means for transiently actuating the suction delivering a time-stamped electrical signal, and in that said signal representative of the presence of a marker is also time-stamped.

4. System for sampling biological tissues according to claim 1 characterized in that said sensor is formed by an annular organic scintillator (610) which detects beta particles and gamma rays associated with a photodetector (630).

5. System for sampling biological tissues according to the preceding claim, characterized in that said annular organic scintillator (610) is surrounded by an inorganic gamma scintillator (640), also annular, which only detects gamma rays, said inorganic gamma scintillator being associated with a second photodetector (660).

6. System for collecting biological tissues according to claim 1 characterized in that it comprises a valve (230) directing the tissues taken to a collection receptacle or to a waste receptacle depending on the information delivered by the detection sleeve (600).

7. System for collecting biological tissues according to claim 1 characterized in that said receptacle (300) is constituted by a barrel comprising a plurality of collection units.

8. System for sampling biological tissues according to claim 1, characterized in that it comprises a pressure switch detecting the activation of the sampling by the excision head.

9. System for sampling biological tissues according to claim 1 characterized in that it comprises an enclosure whose walls comprise lead sheets, for the confinement of said receptacle (300).

10. System for sampling biological tissues according to the preceding claim, characterized in that said enclosure comprises a plurality of housings, each comprising a radiation sensor.

11. System for collecting biological tissues according to claim 9 or 10, characterized in that said enclosure further comprises a housing for receiving a urine bag.

12. System for sampling biological tissues according to claim 1 characterized in that it comprises said electronic circuit which controls a safety alert in the event of non-detection of biomarker.

13. System for sampling biological tissues according to claim 1, characterized in that it further comprises a means for real-time analysis of the tissues on certain histological and biochemical aspects.

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