This product is adaptable to the contours of the face, neck and / or décolletage and is loaded with probiotic microorganism(s).

Abstract

L'OREAL "Article adaptable to the contours of the face, neck and / or décolleté and loaded with probiotic microorganism(s)" The present invention relates to an article, particularly a cosmetic one, adaptable to the contours of the face, neck and / or décolleté, comprising a non-woven backing sheet made of non-synthetic fibers and which, in its dry state, comprises at least one live probiotic microorganism intended to exert a specific action on the skin, said live probiotic microorganism being immobilized in the dry backing sheet in a non-covalent manner and in a form interpenetrated with at least one hydrophilic binding agent having a melting point ranging from 55°C to 140°C. It also relates to a method for preparing said article and the use of said article for treating the skin of the face, neck and / or décolleté.

Description

Title of the invention: Article adaptable to the contours of the face, neck and / or décolletage and loaded with probiotic microorganism(s) Technical field

[0001] The present invention relates to cosmetic treatment devices, in particular cosmetic product applicators. "Cosmetic product" means any composition as defined in Regulation (EC) No 1223 / 2009 of the European Parliament and of the Council of 30 November 2009 on cosmetic products.

[0002] It relates in particular to devices capable of conforming to the shape of the area to be treated and advantageously able to adapt to the contours of the face, neck and / or décolleté, like, for example, a face mask.

[0003] It also relates more particularly to the implementation of such devices for the topical administration of one or more live but conditioned microorganism(s), for example in a lyophilized or atomized state within these devices. Prior art

[0004] The advantage of using live probiotic microorganisms lies in the beneficial properties they can exert on the individual, particularly humans. These properties result either from direct interactions between the individual's cells and the microorganisms, or indirectly, through the release of active substances by these microorganisms or through the regulation of the individual's skin microbial environment due to their presence. It appears that optimizing these beneficial properties would depend on using these microorganisms in a live form.

[0005] However, live probiotic microorganisms may have a stability defect when conditioned, in a solubilized form or dispersed in aqueous medium, over a prolonged period and may therefore be subject to partial degradation during prolonged storage in this form. Description of the invention

[0006] The inventors therefore focused on developing a new type of packaging suitable for preserving these live probiotic microorganisms in a dry form, but also compatible with their regeneration and thus the restoration of their beneficial biological properties for the skin upon contact with the skin area to be treated. In other words, these microorganisms must be efficiently transferred to the skin area to be treated and in an active form.

[0007] This formulation in the dry state at the level of a device, which is also flexible to adapt to the contours of the face, neck and / or décolletage, like for example a face mask, requires in addition to meet a number of additional requirements.

[0008] It is necessary to ensure permanent immobilization of the powdered formulation of these live probiotic microorganisms on the application surface of this device.

[0009] Any phenomenon of agglomeration, such as clumping, of this powder formulation at the level of the device, as well as any risk of degradation of these microorganisms, must be prevented.

[0010] Finally, in addition to all these requirements, there are those of offering consumers an aesthetically attractive device that is also environmentally friendly and whose development involves a simple process and imposes the smallest possible carbon footprint.

[0011] Thus, the invention also aims to provide a device, particularly a cosmetic one, with a better carbon footprint, notably by promoting the use of renewable raw materials and / or those with a high naturalness index and / or of natural origin, while reducing the use of petrochemical-based compounds, such as silicone compounds. Indeed, the formulation of environmentally friendly cosmetic devices, that is, those whose design and development take environmental issues into account, is becoming a major concern in order to contribute to meeting global challenges. It is therefore essential to offer more sustainable cosmetic devices that can thus address these environmental challenges.

[0012] As will be apparent from the following, the inventors have precisely developed such a device, also called a cosmetic article, and a corresponding manufacturing process enabling satisfaction in these terms. Summary of the invention

[0013] Thus, according to one of its aspects, the present invention relates to an article, in particular a cosmetic one, adaptable to the contours of the face, neck and / or décolletage, comprising a non-woven support sheet, made of non-synthetic fibers, characterized in that said support sheet comprises, in the dry state, at least one live probiotic microorganism intended to exert a specific action on the skin, said live probiotic microorganism being immobilized at the level of said support sheet, in the dry state, in a non-covalent manner and in a form interpenetrated in at least one hydrophilic binding agent whose melting point varies from 55 °C to 140 °C.

[0014] For the purposes of the present invention, "interpenetrated form" refers to the immobilization of the asset within a matrix formed essentially or solely from the associated hydrophilic binding agent component, and in particular formed by the fusion of said hydrophilic binding agent. This form of immobilization is therefore different from a deposition solely on the surface of a substrate.

[0015] It is advantageous in several respects.

[0016] It guarantees a long-lasting conditioning of the live probiotic microorganism until its activation and release due to its integration into the hydrophilic binding agent matrix.

[0017] It is suitable for the long-term immobilization of a significant quantity, in particular an effective quantity, of live probiotic microorganisms whose particle size can vary significantly, in particular from 1 µm to 3000 µm, and preferably from 5 µm to 1000 µm. For the purposes of the present invention, "effective quantity" means the minimum quantity sufficient and necessary to obtain the desired effect, namely, in the context of the present invention, the beneficial properties of live probiotic microorganisms for a given indication.

[0018] It is reversible since, during cosmetic use of the article according to the invention, the entirety of the live probiotic microorganism is released in order to exert a specific action on the skin.

[0019] Finally, when the support sheet loaded with dry live probiotic microorganisms comes into contact with a solvent medium, particularly water, no lump formation is advantageously observed. The live probiotic microorganism is delivered in its entirety to the surface of the skin to which the active ingredient-loaded support sheet is applied.

[0020] With regard to the article according to the invention, comprising such a support sheet which contains the live probiotic microorganism and the hydrophilic binding agent, it has a satisfactory visual appearance after manufacture, generally a homogeneous white appearance, and is not subject, during its storage, to a significant yellowing and / or browning phenomenon.

[0021] Preferably, the hydrophilic binding agent used for immobilizing the live probiotic microorganism is a hydrophilic compound of natural origin and as such contributes to the good naturalness index of said article.

[0022] According to a particular embodiment, the present invention relates to an article as defined above, in which:

[0023] - said live probiotic microorganism comprises at least one Lactobacillus plantarum, Lactobacillus rhamnosus, a Lactobacillus plantarum, a Pediococcus pentosaceus, a Staphylococcus epidermidis, or a mixture thereof; and - said hydrophilic binding agent comprises at least rhamnose, an exudate of Aloe Vera leaves, or a mixture thereof.

[0024] According to another particular embodiment, the present invention relates to an article as defined above, in which:

[0025] - said live probiotic microorganism comprises at least one Lactobacillus plantarum,uu Lactobacillus rhamnosus, a Lactobacillus plantarum, a Pediococcus pentosaceus, a Staphylococcus epidermidis, or a mixture thereof; and - said hydrophilic binding agent comprises at least rhamnose.

[0026] The present invention also relates to a kit, particularly a cosmetic one, comprising:

[0027] - an article as defined above, and

[0028] - a hermetically sealed and sterile package, comprising at least one opening system, in particular including aluminium.

[0029] The present invention also relates to a method for preparing an article as defined above, in particular a cosmetic article, adaptable to the shape of the face, neck and / or décolletage, comprising a non-woven support sheet (10) made of non-synthetic fibers on which is immobilized, in a dry state and in a non-covalent manner, at least one live probiotic microorganism (11) intended to exert a specific action on the skin, characterized in that it comprises at least the steps of:

[0030] - to have said live probiotic microorganism (11) available in dry form powdery, in particular freeze-dried or atomized, more particularly freeze-dried;

[0031] - to have at least one hydrophilic binding agent (12) with a melting point varying from 55 °C to 140 °C and in a dry, powdery form;

[0032] - to form, at one face of a non-woven, porous support sheet (10) and in non-synthetic fibres, a deposit, at least partially in depth, of a dry, powdery mixture of at least said live probiotic microorganism with at least said hydrophilic binding agent;

[0033] - expose said deposit to a calendering operation carried out at a temperature and pressures conducive to the melting of said hydrophilic binding agent but inactive with respect to said fibers of said support sheet and compatible with the preservation of biological activity of said living probiotic microorganism, and preferably at a temperature ranging from 80 °C to 140 °C and a pressure ranging from 30 kN / m2 to 10,000 kN / m2, and more preferably ranging from 100 kN / m2 to 5,000 kN / m2; and

[0034] - recover said support sheet loaded with live probiotic microorganism in a form interpenetrated in said hydrophilic binding agent.

[0035] The process according to the invention has the advantage of being compatible with the use of powdered mixtures of very diverse particle sizes and in particular having a particle size ranging from 1 pm to 3000 pm, and preferably ranging from 5 pm to 2000 pm.

[0036] This particle size distribution can be characterized with, for example, a dry Aero disperser such as the Mastersizer 3000 laser diffraction particle size analyzer (Malvern Panalytical).

[0037] Furthermore, the calendering, carried out according to the invention, has the advantage of not altering the fibers of the support sheet in any way and of not totally degrading the live probiotic microorganism so that an effective quantity of the latter remains at a minimum on the article.

[0038] As detailed below, the calendering process considered according to the invention is a very short calendering process, in particular one lasting less than 30 seconds. It can also be described according to the invention as "flash calendering". In the case of continuous pressing, this duration can, by contrast, be on the order of milliseconds.

[0039] In particular, the calendering temperature is adjusted to a value higher than the melting point of the hydrophilic binding agent used, but also lower than the yellowing temperature of the fibers forming the porous, non-woven backing sheet and significantly lower than the temperature that leads to complete degradation of the live probiotic microorganism, both of which are considered simultaneously. Thus, the aesthetic appearance of the non-woven backing sheet is preserved after this process, and the specific action expected of the live probiotic microorganism on the skin is not affected by this calendering operation.

[0040] The present invention also relates to a cosmetic treatment process for a keratinous material in which an article as defined above is applied to said keratinous material.

[0041] The present invention also relates to the use of an article as defined above, or a kit as defined above, for treating the skin of the face, neck and / or décolleté.

[0042] Other features, aspects and advantages of the invention will become apparent from the detailed description that follows.

[0043] In what follows, the expression "at least one" is equivalent to "one or more" and, unless otherwise indicated, the bounds of a domain of values ​​are included in that domain. Brief description of the drawings

[0044] [Fig. 1] schematically and partially represents an example of an installation for implementing the preparation process according to the invention.

[0045] [Fig.2] illustrates the viability of a probiotic microorganism on an article according to the invention. Detailed description Live probiotic microorganism

[0046] For the purposes of the present invention, a "probiotic microorganism" is defined as a live microorganism which, when ingested in adequate amounts, has a positive effect on the health of its host ("Joint FAO / WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotic in Food Including Powder Milk with Live Lactic Acid Bacteria, October 6, 2001"), and which can, in particular, improve the intestinal microbial balance. Specifically, a live probiotic microorganism, when applied to the skin in adequate amounts, has a positive effect on the aesthetic qualities of the skin and / or mucous membranes.

[0047] For the purposes of this invention, "living" means a microorganism capable of reproducing when placed in an environment conducive to such reproduction. For the purposes of this invention, "active" means a microorganism capable of being influenced by its environment and of exerting an influence on its environment through the activation or inhibition of its metabolic pathways, as opposed to "inactive" or "dormant" microorganisms, for example those obtained by freeze-drying, which cannot influence their environment and are subject to little or no influence from it.

[0048] A microorganism in a "dormant state" can become active again. For the purposes of the present invention, "effective quantity" means the minimum quantity sufficient and necessary to obtain the desired effect, namely, in the context of the present invention, the beneficial properties of live probiotic microorganisms with respect to the skin.

[0049] The beneficial properties of live probiotic microorganisms are, for example, an improvement in the skin barrier effect, a restoration of the skin, and / or a reduction of local skin inflammation.

[0050] According to one embodiment, a probiotic microorganism according to the invention can be implemented in an isolated or purified form, i.e. not mixed with one or more compound(s) likely to be associated with it in its original environment.

[0051] According to one embodiment, the live probiotic microorganism may comprise at least, and in particular consist of, a Lactobacillus sp., a Bifidobacterium sp., a Cocci in particular a Staphylococcus sp., a yeast, a spore-forming bacterium, or a mixture thereof, in particular a Lactobacillus sp., a Cocci, or a mixture thereof.

[0052] In particular, the live probiotic microorganism may include at least, and in particular consist of, a Lactobacillus rhamnosus.

[0053] In particular, the live probiotic microorganism may comprise at least, and in particular consist of, a Lactobacillus plantarum, in particular selected from:

[0054] - the Lactobacillus plantarum LB244 Red strain, filed on 13 / 12 / 2018, under DSM accession number 32996, with the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Science Campus Braunschweig-Süd, GERMANY, on behalf of Lactobio ApS;

[0055] - the Lactobacillus plantarum LB356 Red strain, filed on 10 / 04 / 2019, under DSM accession number 33094, with the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Science Campus Braunschweig-Süd, GERMANY, on behalf of Lactobio ApS; and

[0056] - their mixtures.

[0057] In particular, the live probiotic microorganism may comprise at least, and in particular consist of, a Pediococcus pentosaceus, and in particular the strain Pediococcus pentosaceus LB606R, filed on 14 / 12 / 2020, under accession number DSM 33730, with the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Science Campus Braunschweig-Süd, GERMANY, on behalf of Lactobio ApS.

[0058] In particular, the live probiotic microorganism may include at least, and in particular consist of, Staphylococcus epidermidis.

[0059] The probiotic microorganism according to the invention can be present on the support it impregnates, in a dry, powdered state. It can, in particular, be a native powder, but also a powder obtained by conventional drying, spray drying, or freeze-drying, for example. The freeze-drying of the probiotic microorganism can advantageously be carried out in a mixture with maltodextrin. The probiotic microorganism in freeze-dried or spray-dried form can be ground, in particular dry-ground, or sieved to form a powder. Such dry grinding can be carried out, for example, with a conical sieving mill, a ball mill, an impact mill, or an air jet mill.

[0060] For the purposes of the invention, the term dry means that the ingredient concerned, in particular the probiotic microorganism according to the invention as well as the associated hydrophilic binding agent detailed below, contains less than 6% by weight, preferably less than 5% by weight of water, and more preferably less than 2% by weight of water, relative to its total weight.

[0061] A dry ingredient thus exhibits excellent powdery fluidity, conducive to its intimate mixing with other ingredients and to its penetration into the porosity of the fibrous support considered.

[0062] The probiotic microorganism, immobilized according to the invention, is not covalently bound to the fibrous support and is therefore able to free itself by interacting with an aqueous environment.

[0063] According to a particular embodiment, the article according to the invention may comprise from 101 to 1015 CFU of live probiotic microorganism(s), in particular from 102 to 1012 CFU of live probiotic microorganism(s), more particularly from 102 to 1010 CFU of live probiotic microorganism(s), and even more particularly from 105 to 108 CFU of live probiotic microorganism(s). hydrophilic binding agent

[0064] As specified above, the live probiotic microorganism according to the invention is immobilized on a non-woven fibrous support and in a form interpenetrated in at least one hydrophilic binding agent whose melting point varies from 55 °C to 140 °C.

[0065] According to a particular embodiment, the hydrophilic binder is water-soluble and has a melting point ranging from 60 °C to 110 °C.

[0066] According to a first embodiment, the hydrophilic binding agent may comprise at least one plant exudate, in particular with a melting point ranging from 55 °C to 140 °C, especially from 60 °C to 110 °C. This exudate may also be in dry form. In particular, it is water-soluble. Advantageously, it has a melting point below 150 °C, preferably below 130 °C. Advantageously, it is not subject to yellowing at a temperature strictly below 100 °C, preferably strictly below 130 °C.

[0067] In particular, the hydrophilic binding agent comprises at least, and notably consists of, an exudate from Aloe Vera leaves. As a representative example of such a plant exudate, reference may be made to the commercial product Aloe Vera Freeze Dried Powder® 200:1 from Mexi Aloe Lab.

[0068] In particular, the hydrophilic binding agent may include at least one plant exudate, in particular an exudate from Aloe Vera leaves.

[0069] According to a second embodiment, the hydrophilic binding agent may comprise at least one monosaccharide whose melting point varies from 80 °C to 96 °C, in particular from 90 °C to 96 °C.

[0070] It is noted that the monosaccharide advantageously has a melting point below 100 °C, so that it can be efficiently and rapidly melted during the preparation of the backing sheet that can form an article according to the invention, in particular according to the process detailed below. It is advantageously not subject to yellowing or browning at a temperature strictly below 100 °C.

[0071] For the purposes of the invention, a monosaccharide that can be used as a hydrophilic binding agent is in the form of a carbohydrate monomer, which may be a hexose or a pentose, of the L and / or D series, in hydrated or non-hydrated form, including monohydrate, or be a derived form of hexose or pentose.

[0072] According to a particular embodiment, the monosaccharide may be in the form of pyranose and / or furanose.

[0073] According to a particular embodiment, the monosaccharide may comprise at least, and in particular may consist of, rhamnose, ribose or a mixture thereof.

[0074] According to a particular embodiment, the monosaccharide may comprise at least, and preferably may consist of, rhamnose, and in particular present in the form of D-rhamnose and / or L-rhamnose.

[0075] More specifically, a monosaccharide is rhamnose, notably marketed under the name L-rhamnose monohydrate crystalline by the company Danisco.

[0076] Besides its ability to contribute to the immobilization of the active ingredient at the level of the fibrous support, the monosaccharide, according to its chemical nature, can also provide a beneficial cosmetic effect on the skin.

[0077] In particular, rhamnose is also known to stimulate skin regeneration, especially of the epidermis and / or dermis, through improved skin cell renewal, particularly of the epidermis and / or dermis, notably through stimulation of fibroblasts.

[0078] According to another particular embodiment, the monosaccharide may comprise at least, and preferably may consist of, ribose, and in particular present in the form of D-ribose and / or L-ribose.

[0079] More specifically, a monosaccharide is ribose, notably marketed under the name Riboxyl™ by the company Lucas Meyer Cosmetic (IFF).

[0080] According to an advantageous embodiment, said monosaccharide is present in an isolated or purified form, i.e. not mixed with one or more compound(s) that may be associated with it in a source material, such as a plant and / or fruit extract.

[0081] The term "source material" means a material comprising one or more monosaccharides, in the form of carbohydrate monomers and / or polymerized, but in a form mixed with other substances of a different chemical nature. Such a source material may be, in particular, a plant or fruit, or an extract thereof, especially a plant exudate and, in particular, an exudate from Aloe Vera leaves.

[0082] In other words, the article according to the invention comprises at least one monosaccharide in a form different from a plant or fruit extract.

[0083] In particular, the hydrophilic binding agent may comprise at least one monosaccharide with a melting point ranging from 80 °C to 96 °C, notably selected from rhamnose, ribose, or mixtures thereof, said monosaccharide being in particular present in an isolated or purified form, that is to say not mixed with one or more compound(s) likely to be associated with it in a source material, such as a plant and / or fruit extract

[0084] According to a third embodiment, the hydrophilic binding agent may comprise at least one monosaccharide and an additional binding agent, in particular hydrophilic, different from a monosaccharide component, in particular a plant exudate such as, for example, an exudate from Aloe Vera leaves.

[0085] According to a particular embodiment, said live probiotic microorganism and said hydrophilic binding agent are implemented in a live probiotic microorganism / binding agent weight ratio varying from 95 / 5 to 50 / 50, and in particular from 85 / 15 to 70 / 30.

[0086] According to a particular embodiment, the fibrous support is loaded with at least 1 g / m2, or even with at least 10 g / m2 of hydrophilic binding agent(s).

[0087] According to a particular embodiment, an article according to the invention may comprise at least one Lactobacillus plantarum, in particular the Lactobacillus plantarum LB244 Red strain, filed under accession number DSM 32996, and in particular 102 to 1012 cfu of Lactobacillus plantarum.

[0088] According to a particular embodiment, an article according to the invention may include at least one plant exudate, in particular an exudate from Aloe Vera leaves, and 102 to 1012 CFU of Lactobacillus plantarum. fibrous support

[0089] As specified above, the constituent fibers of the fibrous support considered according to the invention for immobilizing at least one living probiotic microorganism are non-synthetic fibers.

[0090] For the purposes of the invention, non-synthetic fibers are fibers not derived from materials exclusively accessible by chemical synthesis such as, for example, a synthetic polymer.

[0091] Thus, the non-synthetic fibers retained according to the invention are advantageously chosen from natural fibers of material of natural origin such as, for example, natural cellulose fibers, fibers generated from at least one material of natural origin, such as, for example, natural cellulose, recycled cellulosic fibers, such as, for example, circulose, bio-based fibers, such as, for example, fibers obtained by bio-fermentation like, for example, microbial cellulose and mycelium moss, and mixtures thereof.

[0092] These fibers are advantageously hydrophilic, absorbent, and moreover not subject to yellowing and / or browning when exposed to a temperature strictly below 150 °C, preferably strictly below 130 °C. They are also non-thermally fusible.

[0093] In particular, non-synthetic fibers are chosen from natural cellulose fibers, regenerated cellulose fibers, wood pulp fibers, paper fibers, fibers from seeds such as cotton and kapok, fibers from plant stems such as flax, hemp, jute and nettle, fibers from leaves such as abaca, fibers from fruits such as coconut and mixtures thereof.

[0094] For the purposes of the invention, "a regenerated cellulose fiber" is a fiber obtained from natural cellulose transformed by a series of chemical operations, in particular as specified below for the Lyocell product.

[0095] The natural cellulose used to manufacture regenerated cellulose fibers can thus come from different wood pulps (beech, white pine, eucalyptus ...), or even from bamboo pulp.

[0096] According to a particular embodiment, the constituent fibers of the fibrous support comprise at least and in particular consist of regenerated cellulose fibers, notably like those of the Lyocell product.

[0097] More specifically, this product is obtained from wood pulp which is mixed with an environmentally friendly organic dissolving agent and water. The mixture is then heated under vacuum to remove the water until the cellulose dissolves and a spinning solution is formed. This solution is then filtered and pressed through spinnerets and placed in a spinning bath where the fibers are formed. Such a product is marketed under the names Tencel® and Veocel®.

[0098] According to a particular embodiment, the fibrous support considered according to the invention contains at least wood pulp fibers.

[0099] According to another particular embodiment, the non-synthetic fibers of the fibrous support considered according to the invention may comprise regenerated cellulose fibers and one or more other fibers selected from wood pulp fibers, paper fibers, fibers from seeds such as cotton and kapok, fibers from plant stems such as flax, hemp, jute and nettle, fibers from leaves such as abaca, fibers from fruits such as coconut and mixtures thereof.

[0100] According to a particular embodiment, the fibrous support contains regenerated cellulose fibers and wood pulp fibers and / or paper pulp fibers and in particular regenerated cellulose fibers and wood pulp fibers.

[0101] Representative examples of fibrous supports consisting of such fiber mixtures include, in particular, the Tricell® non-woven supports from Ecowipes, CAC supports from the company Mondi, Sontara® supports from the company Glatfelter and BioLace® supports from the company Suominen.

[0102] According to a particular embodiment, the non-synthetic fibers constituting said fibrous support consist of regenerated cellulose fibers and wood pulp fibers.

[0103] According to another particular embodiment, the fibrous support contains regenerated cellulose fibers and recycled non-synthetic textile fibers.

[0104] As specified above, the fibrous support is in the form of a sheet or in other words a plate of thin fibrous material.

[0105] The constituent fibers of said support are not woven and therefore their structural organization provides porosity at the level of the fibrous support considered according to the invention. Additional cosmetic ingredients

[0106] An article according to the invention may further comprise an additional cosmetic active ingredient, in particular a hydrophilic one, other than a live probiotic microorganism as required according to the invention. Naturally, a person skilled in the art will take care to choose this or these potential additional cosmetic active ingredients and / or their quantity in such a way that the advantageous properties of the article according to the invention are not, or not substantially, altered by the envisaged addition, particularly with regard to the viability of the live probiotic microorganism.

[0107] For the purposes of the invention, "hydrophilic cosmetic active ingredient" means a water-soluble or water-dispersible cosmetic active ingredient capable of forming hydrogen bonds.

[0108] It can be represented by a single ancillary active ingredient or a mixture of ancillary active ingredients. For the purposes of the invention, the term "hydrophilic ancillary active ingredient" covers both of these alternatives.

[0109] According to a particular embodiment, the hydrophilic auxiliary active ingredient can be immobilized at the level of the fibrous support and retains its properties, in particular its cosmetic activity and its hydrophilicity. In other words, its native properties are little or not affected by its preparation method, in particular as detailed below, which includes a calendering operation carried out in particular at a temperature ranging from 55 °C to 140 °C and a pressure ranging from 30 kN / m² to 10,000 kN / m², and more preferably ranging from 100 kN / m² to 5,000 kN / m².

[0110] Similar to the live probiotic microorganism detailed above, the ancillary cosmetic active ingredient may be present in the carrier it impregnates, in a dry and powdered state. It may, in particular, be a native powder, but also a powder obtained by conventional drying, spray drying, or freeze-drying, for example.

[0111] Preferably, the immobilized accessory cosmetic active ingredient is not covalently bound to the fibrous support and is therefore able to be released from it by interacting with an aqueous medium.

[0112] This additional cosmetic active ingredient may in particular be an antioxidant, healing, moisturizing, depigmenting, and / or anti-aging agent.

[0113] According to a particular embodiment, the ancillary cosmetic active ingredient is water-soluble and selected from among moisturizers, C-glycoside compounds, and in particular hydroxypropyl tetrahydropyrantriol, hyaluronic acid compounds and in particular sodium hyaluronate, salicylic acid compounds and in particular n-octanoyl-5-salicylic acid (capryloyl salicylic acid), ascorbic acid and its derivatives, lactic acid, adenosine and its analogues, plant extracts such as in particular madecassoside, niacinamide compounds and mixtures thereof.

[0114] By way of representative and illustrative example of these categories of ancillary cosmetic active ingredients, the following compounds may be cited in particular: C-glycoside compounds

[0115] In particular, this additional cosmetic active ingredient can be selected from among the C-glycoside compounds, with the following general formula:

[0116] [Chem.l] SX—R

[0117] in which:

[0118] - R denotes an unsubstituted linear alkyl radical in Ci-C4, in particular in Ci-C2, in in particular methyl;

[0119] - S represents a monosaccharide selected from D-glucose, D-xylose, N- acetyl-D-glucosamine or L-fucose, and in particular D-xylose;

[0120] - X represents a group chosen from -CO-, -CH(OH)-, -CH(NH2)-, and preferably a -CH(OH)- group;

[0121] as well as their cosmetically acceptable salts, their solvates such as hydrates and their optical isomers.

[0122] By way of illustration and not limitation of C-glycosides more particularly suitable for the invention, the following compounds may be mentioned in particular:

[0123] - C-beta-D-xylopyranoside-n-propane-2-one;

[0124] - C-alpha-D-xylopyranoside-n-propane-2-one;

[0125] - C-beta-D-xylopyranoside-2-hydroxy-propane;

[0126] - C-alpha-D-xylopyranoside-2-hydroxy-propane;

[0127] - l-(C-beta-D-glucopyranosyl)-2-hydroxy-propane;

[0128] - l-(C-alpha-D-glucopyranosyl)-2-hydroxy-propane;

[0129] - l-(C-beta-D-glucopyranosyl)-2-amino-propane;

[0130] - l-(C-alpha-D-glucopyranosyl)-2-amino-propane;

[0131] - the 3'-(acetamido-C-beta-D-glucopyranosyl)-propane-2'-one;

[0132] - the 3'-(acetamido-C-alpha-D-glucopyranosyl)-propane-2'-one;

[0133] - l-(acetamido-C-beta-D-glucopyranosyl)-2-hydroxyl-propane;

[0134] - l-(acetamido-C-beta-D-glucopyranosyl)-2-amino-propane;

[0135] as well as their cosmetically acceptable salts, their solvates such as hydrates and their optical isomers. Compound of niacinamide

[0136] In particular, this additional cosmetic active ingredient can be chosen from niacinamide (also known as Vitamin B3), N,N-diethylniacinamide, N-picolylniacinamide, N-allylniacinamide. Adenosine and its analogues

[0137] In particular, the additional cosmetic active ingredient may be chosen from adenosine and its analogues such as 2'-deoxyadenosine, 2',3'-isopropoylidene adenosine, toyocamycin, 1-methyladenosine; N-6-Methyladenosine, adenosine N-oxide, 6-Methylmercaptopurine riboside, 6-Chloropurine riboside, 5'-Adenosine monophosphate, 5'-Adenosine diphosphate and 5'-Adenosine triphosphate, phenylisopropyladenosine (PIA), 1-Methylisoguanosine, N-6-Cyclohexyladenosine (CHA), N-6-Cyclopentyladenosine (CPA), 2-Chloro-N-6-Cyclopentyladenosine, 2-Chloroadenosine, N-6-Phenylaminoadenosine, N-6-Phenethyladenosine, 2-p-(2-Carboxethyl)phenethylamino-5'-Nethylcarboxamido-adenosine (CGS-21680), N-10-ethylcarboxamido-adenosine (NECA), 5'(N-cyclopropyl)-carboxamidoadenosine, metrifudil, erythro-9-(2-hydroxy3-nonyl) adenine (EHNA) and iodotubercidin.

[0138] Adenosine is notably available commercially in powder form from the company Pharma Waldhof. Ascorbic acid and derivatives

[0139] In particular, the additional cosmetic active ingredient may be chosen from ascorbic acid, also called Vitamin C, in D or L form, advantageously in L form, and its analogues chosen from its salts, preferably sodium ascorbate, magnesium or sodium ascorbyl phosphate, glycosylated ascorbic acid, its sugar esters and its phosphorylated metal salts.

[0140] The sugar esters of ascorbic acid usable in the invention include, in particular, glycosylated, mannosylated, fructosylated, fucosylated, galactosylated N-acetylglucosamine, N-acetylmuramic derivatives of ascorbic acid and mixtures thereof, and more specifically ascorbyl-2 glucoside or 2-O-α-D-glucopyranosyl of L-ascorbic acid or 6-O-[3-D-galactopyranosyl of L-ascorbic acid. These latter compounds as well as Their preparation processes are described in particular in documents EP 487 404 and EP 425 066.

[0141] The phosphorylated ascorbic acid metal salt can be selected from alkali metal ascorbyl phosphates, alkaline earth metal ascorbyl phosphates and transition metal ascorbyl phosphates.

[0142] The ascorbic acid derivatives may be selected from 5,6-di-O-dimethylsilylascorbate marketed under the reference PRO-AA by Exsymol, potassium salt of dl-alpha-tocopheryl-dl-ascorbyl-phosphate marketed under the reference Sepivital EPC by Senju Pharmaceutical, magnesium ascorbyl phosphate, sodium ascorbyl phosphate marketed under the reference Stay-C 50 by Roche and ascorbyl glucoside marketed by Hayashibara.

[0143] This may in particular refer to the product marketed by BASF under the name Ascorbic Acid / 80 Mesh.

[0144] According to a particular embodiment, the support according to the invention contains at least one vitamin, and in particular vitamin C or one of its derivatives, notably its salts. Hyaluronic acid

[0145] In particular, this additional cosmetic active ingredient can be chosen from hyaluronic acid or one of its derivatives.

[0146] In the context of the present invention, the term "hyaluronic acid or one of its derivatives" includes, in particular, the basic hyaluronic acid motif of formula:

[0147] [Chem.2] j'

[0148] The term “hyaluronic acid or one of its derivatives” also includes, within the scope of the present invention, the linear polymer comprising the polymeric unit described above, in a chain of alternating glycosidic linkages [3(1,4) and [3(1,3)], having a molecular weight (MW) that can vary between 380 and 13,000,000 daltons. This molecular weight depends largely on the source of the hyaluronic acid and / or the preparation methods.

[0149] The term "hyaluronic acid or one of its derivatives" also covers, according to the invention, salts of hyaluronic acid and in particular alkaline salts such as sodium salt and potassium salt.

[0150] In its natural state, hyaluronic acid is present in pericellular gels, in the basic substance of connective tissues of vertebrate organs such as the dermis and the epithelial tissues and in particular in the epidemic, in the synovial fluid of the joint, in the vitreous humor, in the human umbilical cord and in the cristagalli process.

[0151] Thus, the term "hyaluronic acid or one of its derivatives" includes all fractions or subunits of hyaluronic acid having a molecular weight in particular within the molecular weight range recalled above.

[0152] In the context of the present invention, it is preferred to use hyaluronic acid fractions that do not exhibit inflammatory activity.

[0153] As an illustration of the different fractions of hyaluronic acid, reference can be made to the document "Hyaluronan fragments: an information-rich System", R. Stern et al., European Journal of Cell Biology 58 (2006) 699-715, which reviews the listed biological activities of hyaluronic acid according to its molecular weight.

[0154] Finally, the term "hyaluronic acid or one of its derivatives" also includes hyaluronic acid esters, in particular those in which all or part of the carboxylic groups of the acid functions are esterified with alcohols or oxyethylenated alkyls, comprising from 1 to 20 carbon atoms, in particular with a substitution rate at the level of D-glucuronic acid of hyaluronic acid varying from 0.5% to 50%.

[0155] Examples include methyl, ethyl, n-propyl, n-pentyl, benzyl and dodecyl esters of hyaluronic acid. Such esters were notably described in D. Campoccia et al. “Semisynthetic resorbable materials from hyaluronan esterification”, Biomaterials 19 (1998) 2101-2127.

[0156] Hyaluronic acid may be available in particular from Hyactive under the trade name CPN (MW: 10 to 150 kDa), from Soliance under the trade name Cristalhyal (MW: 1.1 x 106), from Bioland under the name Nutra HA (MW: 820000 Da), from Bioland under the name Nutra AF (MW: 69000 Da), from Bioland under the name Oligo HA (MW: 6100 Da) or from Vam Farmacos Metica under the name D Factor (MW: 380 Da).

[0157] Preferably, sodium hyaluronate is used. Salicylic acid compounds

[0158] In particular, this additional cosmetic active ingredient can be chosen from among salicylic acid compounds.

[0159] The salicylic acid compound is preferably selected from salicylic acid and compounds of the following formula:

[0160] [Chem.3] ..OH

[0161] in which:

[0162] - the radical R designates an aliphatic chain having from 2 to 22 carbon atony, saturated, linear, branched or cyclic; an unsaturated chain having from 2 to 22 carbon atoms containing one or more double bonds which may be conjugated; an aromatic ring linked to the carbonyl radical directly or via saturated or unsaturated aliphatic chains having from 2 to 7 carbon atoms; said groups being able to be substituted by one or more substituents, identical or different, selected from (a) halogen atoms, (b) the trifluoromethyl group, (c) hydroxyl groups in free form or esterified by an acid having from 1 to 6 carbon atoms or (d) a carboxyl function in free form or esterified by a lower alcohol having from 1 to 6 carbon atoms;

[0163] - R' is a hydroxyl group;

[0164] as well as their salts derived from a mineral or organic base.

[0165] Among the particularly preferred salicylic acid compounds are n-octanoyl-5-salicylic acid (or capryloyl salicylic acid), n-decanoyl-5-salicylic acid, n-dodecanoyl-5-salicylic acid, n-heptanoyl-5-salicylic acid, and their corresponding salts.

[0166] The salicylic acid compound is preferably chosen from salicylic acid and n-octanoyl-5-salicylic acid, and more preferably is n-octanoyl-5-salicylic acid.

[0167] The salts of the compounds with the formula illustrated above can be obtained by saltification with a mineral or organic base. Examples of mineral bases include alkali or alkaline earth metal hydroxides such as sodium hydroxide, potassium hydroxide, or ammonia.

[0168] Among the organic bases, amines and alkanolamines can be mentioned. Quaternary salts such as those described in patent FR 2 607 498 are particularly interesting.

[0169] This additional active ingredient can also be a plant extract such as madecassoside extracted from Centella asiatica.

[0170] More specifically, this additional cosmetic active ingredient can be chosen from:

[0171] - vitamins and their derivatives, in particular their esters, such as in particular the niacinamide (3-pyridinecarboxamide), nicotinamide (vitamin B3), provitamin B5 also known as panthenol, tocopherol (vitamin E) and its esters (such as tocopherol acetate), ascorbic acid and its derivatives (vitamin C),

[0172] - moisturizers, such as in particular urea, hydroxyureas, glycerol, polyglycerols, glycerol glucoside, diglycerol glucoside, polyglyceryl glucosides, xylityl glucoside and plant extracts, particularly tea, mint, orchid, soy, honey, and especially glycerol,

[0173] - C-glycoside compounds, and preferably hydroxypropyl tetrahydropyrantriol (or proxylane)

[0174] - antioxidant compounds,

[0175] - anti-aging active ingredients, such as, for example, hyaluronic acid compounds, and including sodium hyaluronate, salicylic acid compounds and in particular n-octanoyl-5-salicylic acid (capryloyl salicylic acid), adenosine, c-beta-d-xylopyranoside-2-hydroxypropane and the sodium salt of 3-hydroxy-2-pentylcyclopentylacetic acid,

[0176] - keratolytic agents such as, in particular, lactic acid or glycolic acid, And

[0177] - their mixtures.

[0178] Preferably, an article according to the invention further comprises at least one additional water-soluble cosmetic active ingredient selected from among moisturizers, C-glycoside compounds, and in particular hydroxypropyl tetrahydropyrantriol, hyaluronic acid compounds and in particular sodium hyaluronate, salicylic acid compounds and in particular n-octanoyl-5-salicylic acid (capryloyl salicylic acid), ascorbic acid and its derivatives, lactic acid, adenosine and its analogues, plant extracts such as madecassoside, niacinamide compounds, and mixtures thereof.

[0179] Even more preferably, an article according to the invention comprises at least one anti-aging cosmetic active ingredient, in particular selected from hydroxypropyl tetrahydropyrantriol, or proxylane, adenosine, niacinamide compounds, salicylic acid compounds, ascorbic acid, and mixtures thereof, more particularly selected from hydroxypropyl tetrahydropyrantriol, adenosine, niacinamide compounds, ascorbic acid, and mixtures thereof.

[0180] According to a particular embodiment, the fibrous support considered according to the invention can be loaded with at least 1 g / m2, or even with at least 10 g / m2 of different hydrophilic active ingredient(s) of a live probiotic microorganism.

[0181] In addition to at least one live probiotic microorganism and at least one hydrophilic binding agent according to the invention, the support sheet of said article may contain one or more additional compounds. Additional compounds

[0182] Examples of additional compounds include anti-caking agents, moisture-absorbing agents, powder flow aids, stabilizers, pH regulators, and buffers. Naturally, a person skilled in the art will ensure that any additional compound(s) and / or their quantity are chosen in such a way that the advantageous properties of the article according to the invention are not, or are not substantially, altered by the proposed addition.

[0183] These additional compounds are also present in dry form and in particular in powder form.

[0184] In a particular embodiment, the fibrous support according to the invention further contains at least one anti-caking agent and / or a moisture-absorbing agent and in particular at least silica, a magnesium salt such as magnesium stearate or magnesium carbonate, sodium carbonate, talc, a bamboo exudate (rich in silica) or a mixture thereof.

[0185] More particularly, the anti-caking agent and / or a moisture-absorbing agent is (are) selected from silica, a magnesium salt such as magnesium stearate or magnesium carbonate, talc and mixtures thereof.

[0186] In a particular embodiment, the fibrous support loaded according to the invention further contains at least one stabilizer which may in particular be sodium citrate.

[0187] In a particular embodiment, the fibrous support loaded according to the invention further contains at least one thickener or texturizer.

[0188] According to an advantageous embodiment, the fibrous support of the invention is preferably free of compounds that may be harmful to humans and / or the environment, that is to say, it comprises less than 0.01% by weight, or is even devoid of compounds that may be harmful to humans and / or the environment.

[0189] Thus, it is in particular free from silicone compound and / or ethylenediaminetetraacetic acid (EDTA), and preferably is free from silicone compound and ethylenediaminetetraacetic acid.

[0190] Method for preparing the backing sheet of the article according to the invention

[0191] The support sheet loaded with at least one live probiotic microorganism can be obtained by contacting a dry powder form of said live probiotic microorganism to be immobilized with the non-woven, porous, non-synthetic fiber support in the presence of at least one hydrophilic binding agent whose melting point varies from 55 °C to 140 °C.

[0192] As stated above, this process includes, in particular, the following steps:

[0193] - have said live probiotic microorganism available in dry form powdery;

[0194] - have at least one hydrophilic binding agent with a melting point ranging from 55 °C to 140 °C and in a dry, powdery form;

[0195] - to form, on one face of a non-woven, porous, fibre support sheet non-synthetic, a deposit, at least partially in depth, of a dry, powdery mixture of at least said live probiotic microorganism with at least said hydrophilic binding agent;

[0196] - expose said deposit to a calendering operation carried out at a temperature and pressures conducive to the melting of said hydrophilic binding agent but inactive with respect to said fibers of said support sheet and compatible with the preservation of biological activity of said living probiotic microorganism, and preferably at a temperature ranging from 80 °C to 140 °C and a pressure ranging from 30 kN / m2 to 10,000 kN / m2, and more preferably ranging from 100 kN / m2 to 5,000 kN / m2; and

[0197] - recover said support sheet loaded with live probiotic microorganism in a form interpenetrated in said hydrophilic binding agent.

[0198] The duration of pressure application during the calendering operation can vary, on the order of seconds, depending on the pressure mode, for example continuous or not, and the temperature used. In the case of continuous pressing, it can be on the order of milliseconds.

[0199] This process can be implemented with a fibrous support processing installation, such as that schematically illustrated in [Fig. 1], comprising:

[0200] - a reel 30 from which the non-woven fibrous support 10 to be treated is unwound,

[0201] - a powder mixing unit 15, the powder comprising the microorganism live probiotic 11 and hydrophilic binding agent 12,

[0202] - a powder deposition unit 20 on the support 10,

[0203] - a mechanical processing unit 25, for penetrating deeper into the support 10 the powder previously deposited by unit 20,

[0204] - an inline sliding conveyor belt optionally,

[0205] - a calendering unit 40 disposed between the coils 30 and 50, downstream of the unit 25, comprising for example two rollers between which the support passes, and

[0206] - a reel 50 onto which the support thus treated after calendering is wound.

[0207] The installation also includes all the conventional equipment necessary for the implementation of the process, such as rollers and / or conveyor and guide belts for the non-woven fabric, heating means, means for cutting the finished substrate, etc.

[0208] Where appropriate, the roll 50 can be removed by a cutting and packaging station for the substrate, if this is carried out in-line. a) Formation of the deposit

[0209] As can be seen from the above, the live probiotic microorganism(s) and the hydrophilic binding agent(s) are brought into contact with the face of the support sheet to be impregnated, in the form of a dry powder mixture.

[0210] In particular, the deposit is formed from a powdery mixture of at least the two ingredients required according to the invention and whose particle size may, where appropriate, have been adjusted beforehand to a particle size range which is in accordance with the porosity of the support to be loaded with live probiotic microorganism(s).

[0211] Generally, the particle size of the two deposited ingredients can vary significantly, in particular from 1 µm to 3000 µm, and preferably from 5 µm to 2000 µm. This particle size can be characterized, for example, with a dry Aero disperser such as the Mastersizer 3000 laser diffraction particle size analyzer (Malvern Panalytical).

[0212] According to a particular embodiment, the dry powder mixture is formed by direct deposition onto the face of a non-woven, porous, non-synthetic fiber support sheet of a pre-mix comprising at least said live probiotic microorganism and said hydrophilic binding agent.

[0213] In particular, the powders of each of the ingredients, live probiotic microorganism(s) and hydrophilic binding agent(s), or, if present, one or more other additional compounds, in particular as defined above, are respectively introduced in the form of a dry powder into a powder mixing unit.

[0214] For example, such a mixing unit may be a horizontal or vertical powder homogenizer or grinder, operating in batch or continuous mode, the dry powder mixture being obtained by introducing all the ingredients to be mixed or even ground. More particularly, a batch powder mixer is used to thoroughly mix the ingredients.

[0215] Thus, the present invention relates more particularly to a variant of the process for preparing an article as defined above, comprising at least the steps of:

[0216] - to have a dry, powdery mixture of at least one microorganism live probiotic, with at least one hydrophilic binding agent whose melting point varies from 55 °C to 140 °C;

[0217] - form on one face of a non-woven, porous, fibre support sheet non-synthetic, a deposit, at least partially in depth, of said mixture;

[0218] - expose said deposit to a calendering operation carried out at a temperature and pressures conducive to the melting of said hydrophilic binding agent but inactive with respect to said fibers of said support sheet and preferably at a temperature varying from 80 °C to 140 °C and a pressure varying from 30 kN / m² to 10,000 kN / m², and more preferably varying from 100 kN / m² to 5,000 kN / m²; and

[0219] - retrieve said support sheet loaded with live probiotic microorganism in a form interpenetrated in said hydrophilic binding agent.

[0220] According to another particular embodiment, the dry powder mixture is formed by depositing said live probiotic microorganism and said hydrophilic binding agent separately on the face of a non-woven, porous, non-synthetic fiber support sheet.

[0221] The application of the dry, powdered mixture comprising the two ingredients required according to the invention, or the application of these two ingredients separately, onto one side of the substrate sheet to be loaded, can be carried out using a technique such as dusting, with the aid of a powder funnel or hopper comprising, for example, a volumetric dosing system configured to release a predetermined volume of powder by spraying, for example, using a nozzle. In some cases, it is possible to use localized dusting or to use a stencil to impregnate only certain areas of the substrate.

[0222] Sprinkling is generally carried out over the entire surface of the support to be impregnated.

[0223] The substrate thus coated with the mixture comprising the two ingredients required according to the invention can then be subjected to a mechanical operation, in particular one designed to make the mixture penetrate the porosity of the substrate. Advantageously, the formation of the mixture deposit may further include an impregnation operation, mechanical or otherwise, designed to make said mixture penetrate at least partially into the porosity of said substrate sheet. This operation is carried out prior to the calendering operation.

[0224] The impregnation operation may in particular be chosen from a brushing operation, a scraping operation applied to the surface of said deposit, a vibration operation imposed on said support sheet, a suction operation applied to the face of the support sheet opposite to that supporting said mixture, or even a combination of at least two of these operations.

[0225] For example, this may involve vibrating or shaking the surface deposit of the support to stimulate the movement of the powder deposited on the surface of the support within the porosity of the fibrous support.

[0226] Another impregnation technique can be based on air suction applied to the reverse side of the powder-loaded substrate face. This suction would advantageously allow for the recovery of excess powder.

[0227] The deposit is said to be "partially formed in depth", on the grounds that the powdery mixture may not be entirely distributed in the pores of the fibrous support and that a part may remain on the surface.

[0228] Generally, this deposit is formed at ambient temperature 20 °C + / -5 °C and at atmospheric pressure. b) Calendering operation

[0229] This operation takes place following the deposition of the powdered mixture.

[0230] The parameters of the calendering operation, namely temperature, pressure and time, are in fact adjusted to lead to the melting of the hydrophilic binding agent in question while preventing the manifestation of an undesirable side effect with regard to the live probiotic microorganism and the fibers constituting the support, which are also exposed to this calendering.

[0231] In other words, the calendering operation does not completely alter the integrity or effectiveness of the live probiotic microorganism, nor does it generate a significant yellowing and / or browning of the constituent fibers of the nonwoven backing. The latter remains aesthetically pleasing to the user after calendering.

[0232] This calendering is carried out on the scale of the second and in particular in a duration of less than 50 seconds, and preferably less than 30 seconds. It can then be described according to the invention as "flash calendering".

[0233] According to a particular embodiment, the calendering operation is carried out at a temperature varying from 80 °C to 140 °C and a pressure varying from 30 kN / m2 to 10,000 kN / m2, and more preferably varying from 100 kN / m2 to 5,000 kN / m2.

[0234] This calendering operation is generally carried out using an in-line heated calender.

[0235] At the end of this calendering step, a fibrous support sheet impregnated with at least one hydrophilic active ingredient in the dry state according to the invention is obtained.

[0236] As specified above, the support sheet of the invention can be loaded with 101 to 1015 CFU of live probiotic microorganism(s), in particular with 102 to 1012 CFU of live probiotic microorganism(s), more particularly with 102 to 1010 CFU of live probiotic microorganism(s), and even more particularly with 105 to 108 CFU of live probiotic microorganism(s).

[0237] This impregnation can in particular be characterized according to the method detailed in the experimental part and which is based in particular on the enumeration of the live probiotic microorganisms present at the level of the support sheet loaded according to the invention.

[0238] The article obtained according to the invention is presented in particular in the form of a face mask, a wipe, a disc or pad and in particular in the form of a face mask.

[0239] According to another embodiment, the support sheet has perforations and / or cutouts.

[0240] According to a particular embodiment, the article is associated with a reservoir containing an aqueous medium dedicated to the hydration of said live probiotic microorganism. Kit

[0241] The present invention also relates to a kit, in particular a cosmetic one.

[0242] As mentioned above, this kit includes, in particular:

[0243] - an article as defined above, and

[0244] - a hermetically sealed and sterile package, comprising at least one opening system, in particular including aluminium.

[0245] According to a particular embodiment, the packaging may be in the form of a sachet, a pouch or a box.

[0246] For the purposes of this invention, the term "hermetic" refers to packaging that prevents any exchange between the environment "external" to the packaging and the environment "internal" to the packaging, the article according to the invention being present in the internal environment. The walls and the closure system of the packaging prevent such exchanges.

[0247] The exchanges thus prohibited are gaseous and / or biological exchanges between these two environments. This guarantees the absence of any risk of microbiological contamination of the internal environment, such as contamination by undesirable microorganisms, such as undesirable bacterial contamination. For the purposes of the invention, "undesirable microorganisms" means microorganisms other than the live probiotic microorganisms according to the invention in the claimed article.

[0248] For the purposes of the invention, "internal environment" of the packaging means everything contained within the packaging and "external environment" means everything not contained within the packaging, the internal and external environments being separate.

[0249] According to one embodiment, the closure system is designed for opening, which may or may not be reversible, and includes, for example, a zipper, a clip, a grooved closure such as a ZIP-LOCK™ closure, a partially tearable tab, or a reversibly glued closure. Preferably, the closure system is configured to allow the packaging to be opened by action, including manual action, by a human being without requiring excessive effort.

[0250] In particular, the airtight and sterile packaging includes aluminum. Cosmetic treatment process

[0251] The invention further relates to a cosmetic treatment process, in particular for cleansing and / or caring for the skin, in particular the face, neck and / or décolleté, comprising at least the step of applying to the skin to be treated a fibrous support sheet loaded with hydrophilic active ingredient of an article according to the invention.

[0252] According to a particular embodiment, the step of applying to the skin to be treated a fibrous support sheet loaded with hydrophilic active ingredient of an article according to the invention is carried out in the presence of an aqueous environment or even water.

[0253] According to a particular embodiment, the support sheet of said article may be exposed to a solvent medium before application, then applied to the skin preferably previously impregnated with a solvent medium, in particular wetted by spraying with water, being stretched or not, so as to adapt to the morphology of the user.

[0254] For the purposes of the invention, a "solvent medium" covers water, an aqueous medium, or even an emulsified medium of the direct emulsion type, formed by mixing water or aqueous medium with at least one fatty substance, such as sebum, or even an oil, in particular a vegetable oil such as sweet almond oil.

[0255] In particular, the fibrous support sheet is loaded with a solvent medium or even water prior to its application to the skin. In particular, this loading can be carried out by immersing the support, or by spraying. The treated fibrous support sheet is then applied to the skin, stretched or not, so as to adapt to the user's morphology.

[0256] According to a particular embodiment, the support sheet of said article can be applied in a dry state to skin previously or not impregnated by a solvent medium, in particular wetted by spraying with water, so as to adapt to the morphology of the user.

[0257] According to a particular embodiment, the support sheet of said article can be applied in a dry state to skin previously impregnated with a solvent medium, for example wetted by spraying with water, and subsequently impregnated with a solvent medium, in particular by spraying with water, so as to adapt to the morphology of the user.

[0258] According to the previous variants, the fibrous support sheet is in particular kept on the skin for a determined period and adjusted to the release of the live probiotic microorganism in its activated form.

[0259] Generally this duration can vary from 1 minute to 30 minutes, or even from 2 minutes to 20 minutes, being for example in the order of 10-15 minutes approximately.

[0260] The process according to the invention may in particular be useful for improving the skin's barrier effect, restoring the skin, reducing local inflammation of skin, rehydration treatment, anti-wrinkle, anti-puffiness, or anti-dark circles, or even to provide a plumping effect.

[0261] The following example is presented as an illustration and not as a limitation of the scope of the invention. Example 1 Materials and methods

[0262] The Lactobacillus plantarumLB244 Red strain, filed under accession number DSM 32996, is commercially available from Lactobio ApS, as detailed above.

[0263] The Eugon LT SUP is a commercial product available from Biomérieux.

[0264] MRS (deMan, Rogosa, Sharpe) agar is a commercial product used as a culture medium for microorganisms and is available from MRS BioMérieux under reference AEB621757N.

[0265] Aloe Vera leaf exudate is a commercial powdered product available from Mexi Aloe Laboratorios (marketed by Rossow). Its particle size, expressed in micrometers, is as follows: D10 = 20, D50 = 55, D90 = 1700 and its melting point varies from 67 °C to 108 °C.

[0266] The porous, nonwoven backing is made of Rayon material (1.67 dtex x 38 mm): D-CR053 (MG-1)-LZ. This product is available from the supplier Hangzhou Nbond nonwovens (roll thickness = 0.5 mm and basis weight = 53 g.m²). The fibrous backing sheet used for testing is cut from this nonwoven roll in the form of discs or as a mask. Microorganism enumeration protocol

[0267] The quantification of microorganisms present on a disc after the calendering step is carried out using a counting method comprising the following steps:

[0268] - in a sterile bag, placing the disc or mask in contact with 100 mL of Eugon LT SUP for a contact time of 30 min (+ / -15 min) for neutralization with agitation of the bag at speed 3, for one minute using a BagMixer®;

[0269] - then retrieval of the contents of the sterile bag;

[0270] - cascading dilutions of this content to achieve a dilution factor equal to 10;

[0271] - 0.5 mL of the diluted contents being deposited onto two MRS agar plates respectively different;

[0272] - incubation, at 35°C (+ / -2.5°C) for 48 to 96 hours in an environment anaerobic, MRS agar with deposition of diluted medium;

[0273] - counting microorganisms by performing a colony reading microorganisms by manual counting; and

[0274] - determination of the Log(UFC) value per disk or per mask.

[0275] The above enumeration protocol is validated using the Bacillus subtilis strain. A predetermined quantity of Bacillus subtilis strain, 100 pL, is deposited in liquid form onto a disk. The above enumeration protocol is then applied to such a disk. The experiment is performed twice, and a comparison is made between the Log(CFU) value per disk before and after implementation of the enumeration protocol. The results of this comparison (not shown) demonstrate that the quantity of Bacillus subtilis strain deposited on a disk before the enumeration protocol is identical to that recovered after the enumeration protocol. The enumeration protocol below therefore has no impact on the viability of the microorganisms. Viability loss protocol:

[0276] To quantify the loss of viability of the probiotic microorganism, which may occur during the calendering step, the mask is treated after this step according to the enumeration protocol described above. The loss of viability is characterized by the difference between the count of live probiotic microorganisms on the mask post-calendering, i.e., the quantity of probiotic microorganisms not degraded during the calendering step, minus the known quantity of live probiotic microorganisms deposited on the mask before the calendering step.

[0277] Impregnation of microorganisms on a non-woven mask according to the invention

[0278] A first step consists of grinding 50 g of a mixture of Lactobacillus plantarum LB244 Red and Aloe Vera leaf exudate by centrifugation in a weight ratio of 70:30. This grinding is carried out using a Stuart SR4® centrifugal grinder. The grinder is filled to one-third of its maximum capacity, and then grinding takes place for 1 hour at maximum power to obtain a mixture for storage.

[0279] The resulting powdered mixture is then manually applied by sprinkling onto the fibrous mask, and the powder is then spread evenly and homogeneously over the surface of the fibrous material using a brush. The operation is complete when the quantity of Lactobacillus plantarum LB244 Red strain on the mask reaches 10¹⁰ CFU (i.e., Log CFU equal to 10).

[0280] The mask and its deposit thus formed are then placed on an aluminum plate (15 cm * 15 cm) preheated by contact with the press and covered with a sheet of Teflon®-coated fiberglass fabric. The assembly is then subjected to a pressing process. using a LabEcon 600 heated platen press from Fontijne Presses, at a pressure of 4443 kN / m2, a temperature of 110 °C for 5 seconds

[0281] The enumeration of the Lactobacillus plantarumLB244 Red strain after the pressing process is carried out according to the enumeration protocol described above.

[0282] The impregnation and counting protocols are carried out on three separate masks. The results obtained for these three masks are presented in [Fig.2].

[0283] It appears from [Fig. 2] that the loss of viability in LogUFC of the Lactobacillus plantarum LB244 Red strain at the level of a fibrous mask after hot pressing is -4.8 on average. The effective quantity of live probiotic microorganisms is maintained despite a loss of viability.

Claims

Demands

1. Article, in particular cosmetic, adaptable to the contours of the face, neck and / or décolletage comprising a non-woven support sheet, made of non-synthetic fibers, characterized in that said support sheet comprises, in the dry state, at least one live probiotic microorganism intended to exert a specific action on the skin, said live probiotic microorganism being immobilized at the level of said support sheet, in the dry state, in a non-covalent manner and in a form interpenetrated in at least one hydrophilic binding agent having a melting point ranging from 55 °C to 140 °C.

2. Article according to the preceding claim, wherein said live probiotic microorganism comprises at least, and in particular consists of, a Lactobacillus sp., a Bifidobacterium sp., a Cocci in particular a Staphylococcus sp., a yeast, a spore-forming bacterium, or a mixture thereof, in particular a Lactobacillus sp., a Cocci, or a mixture thereof.

3. Article according to any one of the preceding claims, wherein said live probiotic microorganism comprises at least, and in particular consists of, a Lactobacillus rhamnosus.

4. Article according to any one of the preceding claims, wherein said live probiotic microorganism comprises at least, and in particular consists of, a Lactobacillus plantarum, in particular selected from: - the Lactobacillus plantarum LB244 Red strain, filed under accession number DSM 32996; - the Lactobacillus plantarum LB356 Red strain, filed under accession number DSM 33094; and - mixtures thereof.

5. Article according to any one of the preceding claims, wherein said live probiotic microorganism comprises at least, and in particular consists of, a Pediococcus pentosaceus, and in particular the Pediococcus pentosaceus strain LB606R, filed under accession number DSM 33730.

6. Article according to any one of the preceding claims, wherein said live probiotic microorganism comprises at least, and in particular consists of, Staphylococcus epidermidis.

7. Article according to any one of the preceding claims, comprising from 101 to 1015 CFU of live probiotic microorganism(s), in particular from 102 to 1012 CFU of live probiotic microorganism(s), more particularly from 102 to 1010 CFU of live probiotic microorganism(s), and even more particularly from 105 to 108 CFU of live probiotic microorganism(s).

8. Article according to any one of the preceding claims wherein said live probiotic microorganism and said hydrophilic binding agent are implemented in a live probiotic microorganism / binding agent weight ratio ranging from 95 / 5 to 50 / 50, and in particular from 85 / 15 to 70 / 30.

9. Article according to any one of the preceding claims, wherein said hydrophilic binder is water-soluble and has a melting point ranging from 60 °C to 110 °C.

10. Article according to any one of the preceding claims, wherein said hydrophilic binding agent comprises at least one plant exudate, in particular an exudate from Aloe Vera leaves.

11. Article according to any one of the preceding claims, wherein said hydrophilic binding agent comprises at least one monosaccharide having a melting point from 80 °C to 96 °C, in particular selected from rhamnose, ribose, or mixtures thereof, said monosaccharide being in particular present in an isolated or purified form, i.e. not mixed with one or more compound(s) which may be associated with it in a source material, such as a plant and / or fruit extract.

12. Article according to any one of the preceding claims, wherein: - said live probiotic microorganism comprises at least one Lactobacillus plant arum, one Lactobacillus rhamnosus, one Lactobacillus plantarum, one Pediococcus pentosaceus, one Staphylococcus epidermidis, or a mixture thereof; and - said hydrophilic binding agent comprises at least rhamnose, an exudate of Aloe Vera leaves, or a mixture thereof.

13. Article according to any one of the preceding claims, wherein said backing sheet further contains at least one anti-caking agent and / or a moisture-absorbing agent, and in particular at least silica, a magnesium salt such as magnesium stearate or magnesium carbonate, talc, or a mixture thereof.

14. Article according to any one of the preceding claims wherein said non-synthetic fibers are selected from natural fibers of material of natural origin, fibers generated from at least one material of natural origin, recycled cellulosic fibers, bio-based fibers and mixtures thereof, and in particular include regenerated cellulose fibers and one or more other fibers selected from wood pulp fibers, paper fibers, fibers from seeds such as in particular cotton and kapok, fibers from plant stems such as in particular flax, hemp, jute and nettle, fibers from leaves such as in particular abaca, fibers from fruits such as in particular coconut and mixtures thereof.

15. Article according to any one of the preceding claims in the form of a face mask, a wipe, a disc or a pad and in particular in the form of a face mask.

16. Article according to any one of the preceding claims associated with a reservoir containing an aqueous medium dedicated to the hydration of said live probiotic microorganism.

17. Kit, in particular cosmetic, comprising: - an article as defined according to any one of claims 1 to 16, and - a hermetically sealed and sterile package, comprising at least one opening system, in particular comprising aluminum.

18. A method for preparing an article as defined according to any one of claims 1 to 16, in particular a cosmetic, adaptable to the shape of the face, neck and / or décolletage comprising a non-woven support sheet (10) of non-synthetic fibers on which is immobilized, in a dry and non-covalent manner, at least one live probiotic microorganism (11) intended to exert a specific action on the skin, characterized in that it comprises at least the steps of: - disposing of said live probiotic microorganism (11) in a dry powdered form, in particular lyophilized or atomized, more particularly lyophilized; - have at least one hydrophilic binding agent (12) with a melting point ranging from 55 °C to 140 °C and in a dry powder form; - form, on one face of a non-woven, porous support sheet (10) made of non-synthetic fibers, a deposit, at least partially in depth, of a dry powder mixture of at least said probiotic microorganism living with at least said hydrophilic binding agent; - expose said deposit to a calendering operation carried out at a temperature and pressure conducive to the melting of said hydrophilic binding agent but inactive with respect to said fibers of said support sheet and compatible with the preservation of a biological activity of said living probiotic microorganism, and preferably at a temperature varying from 80 °C to 140 °C and a pressure varying from 30 kN / m2 to 10,000 kN / m2, and more preferably varying from 100 kN / m2 to 5,000 kN / m2;and - recover said support sheet loaded with live probiotic microorganism in a form interpenetrated in said hydrophilic binding agent.;

19. A method according to the preceding claim, wherein the dry powder mixture is formed by direct deposition onto the face of a non-woven, porous, non-synthetic fiber support sheet (10) of a prior mixture comprising at least said live probiotic microorganism (11) and said hydrophilic binding agent (12).

20. A method according to claim 18, wherein the dry, powdery mixture is formed by depositing said live probiotic microorganism and said hydrophilic binding agent separately onto the face of a non-woven, porous, non-synthetic fiber support sheet.

21. Non-therapeutic use of an article as defined according to any one of claims 1 to 16, or of a kit as defined according to claim 17, for cosmetically treating the skin of the face, neck and / or décolleté.

22. Use according to claim 21, characterized in that the backing sheet of said article is exposed to a solvent medium before application, then applied to skin preferably previously impregnated with a solvent medium, in particular wetted by spraying water, whether stretched or not, so as to adapt to the user's morphology.

23. Use according to claim 21, characterized in that the support sheet of said article is applied in a dry state to skin previously or not impregnated with a solvent medium in particular wetted by spraying with water, so as to adapt to the morphology of the user.

24. Use according to claim 21, characterized in that the backing sheet of said article is applied in a dry state to skin previously impregnated with a solvent medium, for example wetted by spraying with water, and subsequently impregnated with a solvent medium, in particular by spraying with water, so as to adapt to the morphology of the user.

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