Cosmetic use of an extract of at least one bacterium of the species Staphylococcus epidermidis as a healing agent

Abstract

Cosmetic use of an extract of at least one bacterium of the species Staphylococcus epidermidis as a healing agent. The present invention relates to the non-therapeutic cosmetic use of an extract of at least one bacterium of the species Staphylococcus epidermidis, which can be obtained by at least one step of culturing at least one bacterium of the species Staphylococcus epidermidis, in the presence of a culture medium comprising i) at least one sugar, ii) at least one peptone of vegetable origin, and / or at least one yeast extract, and optionally iii) at least one microelement, as a healing active, and / or to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably the skin.The present invention also relates to the non-therapeutic cosmetic use of a biomass extract of at least one bacterial strain of the species Staphylococcus epidermidis chosen from the group consisting of bacteria deposited at the CNCM under order numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695, as a healing active, and / or to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably the skin, as well as a cosmetic treatment process for keratinous materials using said extract in a composition, in particular a cosmetic one.

Description

Title of the invention: Cosmetic use of an extract of at least one bacterium of the species Staphylococcus epidermidis as a healing agent. Technical field

[0001] The present invention relates to the field of keratinous material care, in particular skin care, more particularly skin healing and regeneration.

[0002] The present invention relates to the non-therapeutic cosmetic use of an extract of at least one bacterium of the species Staphylococcus epidermidis as a healing agent, and / or to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably skin, obtainable by a particular process. The present invention also relates to the non-therapeutic cosmetic use of an extract of at least one specific bacterial strain of the species Staphylococcus epidermidis as a healing agent, and / or to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably skin.The present invention further relates to a cosmetic treatment process for keratinous materials using said extract within a composition to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably the skin. Previous technique

[0003] Human skin is composed of two compartments: a deep compartment, the dermis, and a superficial compartment, the epidermis. The epidermis is in contact with the external environment, and its role is to protect the body from dehydration and external chemical or mechanical aggressions.

[0004] Skin regeneration or healing is an area of ​​intense scientific research aimed at improving skin repair following external aggression or when the barrier function is compromised. For example, the skin barrier can be disrupted after a cosmetic procedure such as laser treatments and chemical peels, or in the presence of external aggressions such as irritants (cleansing agents) or mechanical stresses (friction, shaving or waxing), thermal or climatic imbalances (cold, dryness, radiation), xenobiotics (allergens), or internal aggressions such as psychological stress. Following such external aggressions, a repair process is triggered, aiming to rapidly and completely restore this barrier. This physiological process depends on complex biological mechanisms involving numerous cellular and molecular interactions.

[0005] There is a need for compositions that promote skin healing, and in particular, that accelerate skin healing.

[0006] In parallel, the formulation of environmentally friendly cosmetic products, that is to say, products whose design and development take environmental issues into account, is becoming a major concern in order to help meet global challenges. It is therefore essential to offer ingredients that address these environmental issues.

[0007] In this context, it is important to develop new environmentally friendly ingredients and / or active ingredients, particularly those of natural origin, especially bio-based and / or from sustainable sources not derived from petrochemicals and / or biodegradable and / or whose extraction process requires low energy and water consumption, in order to offer compositions that reduce the environmental impact of products. Description of the invention

[0008] The present invention aims to provide a cosmetic composition meeting all these requirements.

[0009] Indeed, the inventors made the surprising discovery that an extract of a bacterium of the species Staphylococcus epidermidis, in particular a bacterial extract in the form of a lysate of a bacterial strain CNCM-I 5691 of the species Staphylococcus epidermidis, makes it possible to accelerate the migration of keratinocytes, and is therefore useful for the regeneration and / or healing of keratinous materials such as skin, in particular epithelial regeneration and / or healing. Summary of the invention

[0010] The present invention relates primarily to the non-therapeutic cosmetic use of an extract of at least one bacterium of the species Staphylococcus epidermidis, which can be obtained, obtained, or directly obtained by at least one culture step of at least one bacterium of the species Staphylococcus epidermidis, preferably at least one bacterial strain of the species Staphylococcus epidermidis selected from the group of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694, and 1-5695, in the presence of a culture medium comprising i) at least one sugar, ii) at least one peptone of plant origin and / or at least one yeast extract, and optionally iii) at least one microelement, as a healing active ingredient, and / or to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably the skin.

[0011] Another of its objects is the non-therapeutic cosmetic use of a biomass extract of at least one bacterial strain of the species Staphylococcus epidermidis chosen from the group consisting of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694 and 1-5695, as a healing active, and / or to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably the skin.

[0012] The present invention further relates to a non-therapeutic cosmetic treatment method for keratinous materials, preferably skin, comprising the application, preferably topically, to keratinous materials, preferably skin, of a composition comprising in a physiologically acceptable medium an extract according to the invention, to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably skin.

[0013] Other features, aspects and advantages of the invention will become apparent from the detailed description that follows. Definitions

[0014] The term “keratinous materials” means the skin of the body or face, the lips, the mucous membranes, the eyelashes, the nails, and the hair of a human being.

[0015] By "skin" we mean the entire skin of the body and the scalp of a human being and preferably the skin of the face, décolletage, neck, arms and forearms, hands or even more preferably the skin of the face (in particular the forehead, nose, cheeks, lips, chin), décolletage and neck.

[0016] By "physiologically acceptable medium" is meant a medium particularly suitable for the application of a composition of the invention on keratinous materials, in particular the skin.

[0017] For the purposes of the present invention, the term "promote" refers to the enhancement of the effects of the phenomenon. For example, promoting skin regeneration may lead to greater skin regeneration than without the use of the extract according to the invention.

[0018] For the purposes of the present invention, the term "acceleration" refers to the acceleration of the phenomenon. For example, accelerating skin regeneration can lead to the same amount of regenerated skin, but in less time than without the use of the extract according to the invention.

[0019] For the purposes of the present invention, "regeneration of keratinous materials" or "regeneration of the skin" means the innate ability of living organisms to renew their keratinous materials or their skin, by a process of cell renewal, in particular of re-epithelialization of the skin.

[0020] For the purposes of the present invention, "keratinous material healing" or "skin healing" refers to the innate capacity of living organisms to repair their keratinous materials or their skin, through a process of cell migration or migration of skin cells, in particular migration of keratinocytes and / or fibroblasts.

[0021] In the context of the invention, "keratinous material healing" or "skin healing" may follow an injury to keratinous materials, in particular a skin injury, or may follow a compromised skin barrier function, for example, during or after cosmetic surgery procedures.

[0022] Advantageously, skin healing is the healing of a skin lesion or skin wound.

[0023] By "topical use or application" is meant a use or application on the surface of the keratinous materials concerned, preferably on the surface of the skin concerned.

[0024] “Staphylococcus epidermidis”, or white staph, is a bacterium commensal of humans, forming part of the cutaneous and mucous membrane flora of almost the entire human population.

[0025] The Staphylococcus epidermidis (S. epidermidis) strain CNCM 1-5688 (1-5688), is the strain filed by L'Oréal with the CNCM on June 3, 2021 under accession number CNCM 1-5688 (1-5688).

[0026] The Staphylococcus epidermidis (S. epidermidis) strain CNCM 1-5689 is the strain filed by L'Oréal with the CNCM on June 3, 2021 under accession number CNCM 1-5689 (1-5689).

[0027] The Staphylococcus epidermidis (S. epidermidis) strain CNCM 1-5904 is the strain filed by L'Oréal with the CNCM on September 21, 2022 under accession number CNCM 1-5904 (1-5904).

[0028] The Staphylococcus epidermidis (S. epidermidis) strain CNCM 1-5691 is the strain filed by L'Oréal with the CNCM on June 3, 2021 under accession number CNCM 1-5691 (1-5691).

[0029] The Staphylococcus epidermidis (S. epidermidis) strain CNCM 1-5692 is the strain filed by L'Oréal with the CNCM on June 3, 2021 under accession number CNCM 1-5692 (1-5692).

[0030] The Staphylococcus epidermidis (S. epidermidis) strain CNCM 1-5693 is the strain filed by L'Oréal with the CNCM on June 3, 2021 under accession number CNCM 1-5693 (1-5693).

[0031] The Staphylococcus epidermidis (S. epidermidis) strain CNCM 1-5694 is the strain filed by L'Oréal with the CNCM on June 7, 2021 under accession number CNCM 1-5694 (1-5694).

[0032] The Staphylococcus epidermidis (S. epidermidis) strain CNCM 1-5695 is the strain filed by L'Oréal with the CNCM on June 7, 2021 under accession number CNCM 1-5695 (1-5695).

[0033] For the purposes of this invention, "extract of at least one bacterium" means the culture supernatant of said bacteria, the biomass obtained after culture of said bacteria, or extracts of biomass obtained by treatment of this biomass.

[0034] The bacterial extract according to the invention can be in a live, semi-active or inactivated, dead form, preferably the bacterial extract according to the invention is in an inactivated or dead form.

[0035] Advantageously, said extract is distinct from the culture supernatant of said bacteria.

[0036] Advantageously, said extract of at least one bacterium of the species Staphylococcus epidermidis, a lysate of at least one bacterium of the species Staphylococcus epidermidis.

[0037] For the purposes of this invention, "lysate" means a material obtained as a result of the alteration of bacterial cells, such as the destruction or breakage of bacterial cells, in particular of cell walls and / or membranes, by a phenomenon known as cell lysis, thereby causing the release of intracellular biological constituents naturally contained in the cells of the microorganism in question.

[0038] For the purposes of the present invention, the term lysate is used interchangeably to designate the entire lysate obtained by lysis of the microorganism concerned or only a fraction thereof.

[0039] The lysate is therefore formed in whole or in part from intracellular biological constituents and from constituents of cell walls and membranes.

[0040] More specifically, it contains the cytoplasmic cellular fraction containing enzymes such as lactic acid dehydrogenase, phosphatases, phosphoketolases, transaldolases, and metabolites. By way of illustration, the constituents of cell walls include peptidoglycan, murein or mucopeptide, and teichoic acid, and the constituents of cell membranes are composed of glycerophospholipids.

[0041] This cell lysis can be accomplished by various technologies well known to those skilled in the art, such as osmotic shock, thermal shock, ultrasound, centrifugation, preferably thermal shock such as freezing followed by thawing. Detailed description

[0042] The present invention relates primarily to the non-therapeutic cosmetic use of an extract of at least one bacterium of the species Staphylococcus epidermidis, which can be obtained, obtained, or directly obtained by at least one culture step of at least one bacterium of the species Staphylococcus epidermidis, preferably an extract of at least one bacterial strain of the species Staphylococcus epidermidis selected from the group of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694, and 1-5695, in the presence of a culture medium comprising i) at least one sugar, ii) at least one peptone of vegetable origin and / or at least one yeast extract, and optionally iii) at least one microelement, as a healing active ingredient, and / or to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably in the skin.

[0043] The extract of at least one bacterium of the species Staphylococcus epidermidis according to the invention is preferably selected from i) the culture supernatant of said bacterium, ii) the biomass obtained after culture of said bacterium, and iii) the biomass obtained after culture of said bacterium having undergone chemical or physical treatment such as cell lysis treatment and / or stabilization treatment. Said biomass iii) preferably being in the form of a lysate.

[0044] Preferably, said chemical or physical treatment is selected from osmotic shock, thermal shock, ultrasound, centrifugation, autoclaving, ultra-high temperature (UHT technique), high-pressure sterilization, lyophilization, gamma radiation, and freezing.

[0045] Preferably, said Staphylococcus epidermidis bacterium cultured is a Staphylococcus epidermidis bacterial strain selected from the group of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694 and 1-5695, preferably a Staphylococcus epidermidis bacterial strain selected from the group of bacteria deposited at the CNCM under order numbers 1-5688, 1-5691, 1-5904, 1-5692, 1-5693, 1-5694 and 1-5695, even more preferably a Staphylococcus epidermidis bacterial strain deposited at the CNCM under order number 1-5691.

[0046] Advantageously, said culture medium is free of peptone of animal origin, preferably, said culture medium is free of casein peptone.

[0047] Advantageously, said culture medium comprises less than 4 g / L of sodium chloride, preferably less than 1 g / L by weight of sodium chloride, even better less than 0.5 g / L of sodium chloride or even is free (0 g / L) of sodium chloride.

[0048] Advantageously, said culture medium is distinct from the TSB (Tryptic Soy Broth) medium.

[0049] For the purposes of this invention, "peptone" refers to a product of the hydrolysis reaction of protein-rich food products. This hydrolysis can be chemical or enzymatic. Three types of protein raw materials are used for the manufacture of peptones: peptones of animal origin, such as acid casein and whey; peptones of plant origin, such as soybeans, cotton, corn, broad beans, and wheat; and peptones of mycelial origin. In addition to the origin of the proteins, peptones can be further classified according to their type of hydrolysis: chemical hydrolysis, typically using hydrochloric acid, which is then neutralized with sodium hydroxide; and enzymatic hydrolysis, using proteolytic enzymes, either digestive enzymes such as pepsin, trypsin, and pancreatin, or non-digestive enzymes such as papain.

[0050] According to the present invention, the peptone used is a peptone of vegetable origin, preferably a peptone of vegetable origin chosen from peptones of soy, cotton, corn, bean, or wheat.

[0051] Advantageously, the peptone used is a peptone obtained by enzymatic hydrolysis, preferably using proteolytic enzymes, more preferably using papain.

[0052] According to a preferred embodiment, the peptone used is a papainic soy peptone.

[0053] Preferably, said culture medium comprises in water, preferably distilled water: i. at least one sugar present in the culture medium at a concentration ranging from 0.5 to 30 g / L, and ii. at least one peptone of plant origin present in the culture medium at a concentration of 0.5 to 15 g / L, and / or at least one yeast extract present in the culture medium at a concentration of 0.5 to 15 g / L, and iii. possibly at least one microelement present in the culture medium at a concentration ranging from 0.05 to 6 mg / L, and iv. possibly at least one calcium salt present in the culture medium at a concentration ranging from 0.01 to 1 g / L.

[0054] Preferably, said culture medium comprises in water, preferably distilled water: i. at least one sugar present in the culture medium at a concentration ranging from 0.5 to 7 g / L, and ii. at least one peptone of plant origin present in the culture medium at a concentration of 0.5 to 5 g / L, and / or at least one yeast extract present in the culture medium at a concentration of 0.5 to 5 g / L, and iii. possibly at least one microelement present in the culture medium at a concentration ranging from 0.5 to 6 mg / L, and iv. possibly at least one calcium salt present in the culture medium at a concentration ranging from 0.01 to 0.1 g / L.

[0055] More preferably, said culture medium comprises in water, preferably distilled water: i. at least one sugar present in the culture medium at a concentration ranging from 0.5 to 7 g / L, said sugar being selected from glucose, sucrose, and mixtures thereof, and ii. at least one peptone of plant origin present in the culture medium at a concentration of 0.5 to 5 g / L, said peptone being soy papain peptone, and at least one yeast extract present in the culture medium at a concentration of 0.5 to 5 g / L, said yeast extract being an autolytic yeast extract, and iii. at least one microelement present in the culture medium at a concentration ranging from 0.5 to 6 mg / L, the microelement(s) being chosen from among the Heller microelements, and iv. at least one calcium salt present in the culture medium at a concentration of 0.01 to 0.1 g / L, said calcium salt being calcium chloride.

[0056] Even more preferably, said culture medium comprises in water, preferably distilled water: i. at least one sugar present in the culture medium at a concentration ranging from 0.5 to 7 g / L, said sugar being glucose, and ii. at least one peptone of plant origin present in the culture medium at a concentration of 0.5 to 5 g / L, said peptone being soy papain peptone, and at least one yeast extract present in the culture medium at a concentration of 0.5 to 5 g / L, said yeast extract being an autolytic yeast extract, and iii. at least one microelement present in the culture medium at a concentration ranging from 0.5 to 6 mg / L, the microelement(s) being chosen from among the Heller microelements, and iv. at least one calcium salt present in the culture medium at a concentration of 0.01 to 0.1 g / L, said calcium salt being calcium chloride.

[0057] Heller's microelements, the composition of which is given below, were described by Heller, Ann Sci. Nat. Biol. Veg. 14:1 223 (1953). They are mixtures of various mineral elements that Heller recommended not for culturing bacteria, but for the nutrition of plant tissues cultured in vitro. It should be noted here that no attempt has been made to determine whether Heller's microelements are all indispensable or useful for the culture of said bacterium. However, it has been found that Heller's microelements, used together in combination with the other aforementioned constituents, do indeed allow the culture of the bacteria in question.

[0058] The composition of Heller's microelements, for 1 liter of distilled water, is as follows:

[0059] [Tables 1] ZnSO4, 7 H2O 1g MnSO4, H2O 0.076 g CuSO4, 5H2O 0.003 g Kl 0.010 g A1CI3, 6H2O 0.050 g NiCl2, 6H2O 0.030 g

[0060] The culture step can be carried out at the appropriate temperature suitable for the bacterial species being cultured. Generally, this temperature is between 18 and 40°C. The pH of the culture medium is preferably between 5 and 8.

[0061] When the bacterial culture has reached a terminal stage, that is, generally when the biomass has reached the end of its exponential growth, the biomass can be separated from the culture medium. Preferably, after the culture stage, a step is performed to separate the bacteria, also called biomass, from the culture medium. This separation step can be achieved by any conventional technique, preferably by centrifugation, filtration, or coagulation, particularly with an alcohol such as ethanol, isopropanol, isobutanol, and mixtures thereof. More preferably, the separation step is a separation of the bacteria (or biomass) from the culture medium by centrifugation.

[0062] The supernatant fraction of this biomass can also be filtered in a sterile container to remove suspended particles, for example using a 0.2 µm filter, and / or preferably stabilized by heat treatment, for example by autoclaving between 115°C and 121°C for 15 to 40 minutes. This supernatant fraction can also be transferred aseptically into a sterile container. According to a particular embodiment of the invention, the supernatant fraction thus obtained is used as a wound-healing agent according to the invention.

[0063] Preferably, after the separation step, a cell lysis step is carried out. This cell lysis step can be achieved by any conventional technique well known to those skilled in the art, preferably by osmotic shock, thermal shock, ultrasound, or centrifugation, preferably by thermal shock such as freezing followed by thawing.

[0064] Preferably, after the step of separating the bacteria (or biomass) from the culture medium, a step of stabilizing the bacteria (or biomass) is carried out.

[0065] Preferably, after the cell lysis step, a stabilization step is carried out on the lysate obtained at the end of the cell lysis step.

[0066] This stabilization step can then be carried out by any process well known to those skilled in the art, preferably by autoclaving, ultra-high temperature (UHT technique), high-pressure sterilization, lyophilization, gamma radiation or freezing, preferably by autoclaving, even more preferably by autoclaving carried out at a temperature between 115°C and 21°C and / or for a time between 15 and 40 minutes.

[0067] In a preferred embodiment, the stabilization step is carried out by autoclaving at a temperature between 115°C and 21°C and / or for a time between 15 and 40 minutes.

[0068] In another preferred embodiment, the stabilization step is carried out by lyophilization.

[0069] An example of the preparation of a usable extract according to the invention is given elsewhere in the examples.

[0070] The present invention further relates to the non-therapeutic cosmetic use of a biomass extract of at least one bacterial strain of the species Staphylococcus epidermidis chosen from the group consisting of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694 and 1-5695, as a healing active, and / or to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably the skin.

[0071] Advantageously, said extract is a lysate of at least one bacterial strain of the species Staphylococcus epidermidis selected from the group consisting of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694 and 1-5695.

[0072] Advantageously, said extract is an extract of at least one bacterial strain of the species Staphylococcus epidermidis selected from the group consisting of bacteria deposited at the CNCM under order numbers 1-5688, 1-5691, 1-5904, 1-5692, 1-5693, 1-5694 and 1-5695.

[0073] Advantageously, said extract is a lysate of at least one bacterial strain of the species Staphylococcus epidermidis selected from the group consisting of bacteria deposited at the CNCM under order numbers 1-5688, 1-5691, 1-5904, 1-5692, 1-5693, 1-5694 and 1-5695.

[0074] More preferably, said extract is an extract of at least one bacterial strain of the species Staphylococcus epidermidis deposited at the CNCM under order number 1-5691.

[0075] Even more preferably, said extract is a lysate of at least one bacterial strain of the species Staphylococcus epidermidis deposited at the CNCM under order number 1-5691.

[0076] Advantageously, said extract comprises amino acids, preferably said amino acids are present in a content ranging from 20% to 50% by weight relative to the total weight of the dry extract, more preferably said amino acids are present in a content ranging from 25% to 45% by weight relative to the total weight of the dry extract.

[0077] Amino acids can be selected from aspartic acid, threonine, serine, glutamic acid, proline, valine, alanine, cystine, glycine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, arginine, histidine, and mixtures thereof.

[0078] For the purposes of the present invention, "amino acids" means both free amino acids and those contained in proteins.

[0079] Advantageously, said extract comprises lipids, preferably said lipids are present in a content ranging from 1% to 10% by weight relative to the weight of the dry extract, more preferably said lipids are present in a content ranging from 3% to 7% by weight relative to the weight of the dry extract.

[0080] Advantageously, said extract comprises sugars, preferably said sugars are present in a content ranging from 1% to 10% by weight relative to the weight of the dry extract, more preferably said sugars are present in a content ranging from 3% to 7% by weight relative to the weight of the dry extract.

[0081] Advantageously, said extract comprises mineral matter, preferably said mineral matter is present in a content ranging from 10% to 30% by weight relative to the weight of the dry extract, more preferably said mineral matter is present in a content ranging from 15% to 25% by weight relative to the weight of the dry extract.

[0082] According to a preferred embodiment, said extract comprises: - 20% to 50% by weight of amino acids relative to the total weight of the dry extract, more preferably, 25% to 45% by weight of amino acids relative to the total weight of the dry extract, and - 1% to 10% by weight of lipids relative to the weight of the dry extract, more preferably 3% to 7% by weight of lipids relative to the weight of the dry extract, and - 1% to 10% by weight of sugars relative to the weight of the dry extract, more preferably 3% to 7% by weight of sugars relative to the weight of the dry extract, and - 10% to 30% by weight of mineral matter relative to the weight of the dry extract, more preferably 15% to 25% by weight of mineral matter relative to the weight of the dry extract.

[0083] The extract according to the invention can be used pure or in a composition, in particular cosmetic, in particular for the care of keratinous materials, further comprising a physiologically acceptable medium.

[0084] Advantageously, said extract is present in said composition in a content ranging from 0.0001% to 20% by weight of dry matter relative to the total weight of the composition, preferably in a content ranging from 0.001% to 10% by weight of dry matter, more preferably in a content ranging from 0.01% to 5% by weight of dry matter, even better in a content ranging from 0.1% to 1% by weight of dry matter relative to the total weight of the composition.

[0085] Of course, said composition may include an extract of a single type of bacterial strain as indicated above or a mixture of extracts of at least two or more bacterial strains as listed above.

[0086] Said composition is preferably free from extract of strain different from a strain such as previously listed.

[0087] The physiologically acceptable medium is generally adapted to the nature of the support on which the composition is to be applied, as well as to the appearance in which the composition is to be packaged.

[0088] The physiologically acceptable medium may include water and optionally one or more solvent(s) miscible with water.

[0089] According to a preferred embodiment, said composition comprises at least 20% by weight of water, in particular at least 40% by weight of water relative to the total weight of said composition. Preferably, said composition has a water content ranging from 20% to 95% by weight, even more preferably from 40% to 90% by weight relative to the total weight of the composition.

[0090] Among the water-miscible organic solvents (at room temperature such as 25 °C) we can mention lower monoalcohols having 1 to 5 carbon atoms such as ethanol and isopropanol, glycols having 2 to 8 carbon atoms such as ethylene glycol, hexylene glycol, propylene glycol, 1,3-butylene glycol, pentylene glycol, dipropylene glycol, and glycerin.

[0091] The water-miscible organic solvent(s) may be present at a concentration of 0.01% to 30% by weight, preferably from 0.1% to 20% by weight and more preferably, from 1% to 15% by weight, relative to the total weight of the composition.

[0092] According to a preferred embodiment, the pH of the composition according to the invention is between 4 and 7.5, in particular between 4.5 and 7, and in particular between 4.7 and 6.5.

[0093] Said composition may also include any water-soluble or water-dispersible compound such as gelling agents, film-forming polymers, thickeners, surfactants and mixtures thereof.

[0094] Said composition may also include at least one fatty substance such as one or more oil(s).

[0095] According to one embodiment, the composition may comprise from 5% to 80% by weight of fats, preferably oil(s), and preferably from 10% to 60% of fats, preferably oil(s), by weight relative to the total weight of said composition.

[0096] The term "oil" refers to any fatty substance in liquid form at room temperature (20-25°C) and atmospheric pressure. These oils may be of vegetable, mineral, or synthetic origin.

[0097] Examples of usable oils in said composition include:

[0098] - hydrocarbon oils of vegetable origin;

[0099] - synthetic esters and ethers, particularly of fatty acids, such as oils of formulas R1COOR2 and R1OR2 in which RI represents the remainder of a fatty acid containing 8 to 29 carbon atoms, and R2 represents a hydrocarbon chain, branched or unbranched, containing 3 to 30 carbon atoms;

[0100] - linear or branched hydrocarbons, of mineral or synthetic origin;

[0101] - fatty alcohols having from 8 to 26 carbon atoms;

[0102] - partially hydrocarbon and / or silicone fluorinated oils;

[0103] - silicone oils;

[0104] - their mixtures.

[0105] The term hydrocarbon oil in the list of oils cited above means any oil consisting mainly of carbon and hydrogen atoms, and possibly ester, ether, fluorinated, carboxylic acid and / or alcohol groups.

[0106] According to a preferred embodiment of the invention, the composition used in the context of the invention is a water-in-oil (W / O) or oil-in-water (O / W) emulsion, preferably oil-in-water (O / W). The proportion of fatty substances in the emulsion can range from 5 to 80% by weight, and preferably from 10 to 60% by weight relative to the total weight of the composition.

[0107] The composition may include at least one emulsifier. Emulsions generally contain at least one emulsifier selected from amphoteric, anionic, cationic, or non-ionic emulsifiers, used alone or in mixtures. and possibly a co-emulsifier. Emulsifiers are chosen appropriately depending on the emulsion to be obtained (W / O or W / O).

[0108] The emulsifier and co-emulsifier are generally present in the composition, in a proportion ranging from 0.3 to 20% by weight, and preferably from 0.5 to 10% by weight relative to the total weight of the composition.

[0109] Preferably, said composition may include at least one additional ingredient selected from the group consisting of thickeners, perfumes, preservatives, cosmetic actives other than the extract according to the invention such as healing actives other than the extract according to the invention, emollients, fillers and mixtures thereof.

[0110] Such an additional ingredient may represent from 0.0001% to 20%, preferably from 0.01% to 10% and better still, from 0.1% to 5% by weight, relative to the total weight of the composition.

[0111] Of course, a person skilled in the art will take care to choose this or these additional ingredients and / or their quantity in such a way that the advantageous properties of the strains according to the invention are not, or substantially not, altered by the envisaged addition.

[0112] Said composition may be presented in all the galenic forms normally used in the cosmetic field.

[0113] It may be in particular in the form of an aqueous, hydroalcoholic, possibly gelled solution, a lotion-type dispersion possibly two-phase, an oil-in-water or water-in-oil or multiple emulsion, an aqueous gel, or a dispersion of oils in an aqueous phase, particularly using spherules, these spherules being polymeric particles or, preferably, ionic and / or non-ionic lipid vesicles. It may be of a more or less fluid liquid consistency.

[0114] Preferably, a composition according to the invention is distinguished from compositions intended primarily for detergent purposes with respect to skin, hair and / or mucous membranes, such as soaps, shampoos and shower gels for washing and / or cleaning.

[0115] Said composition is preferably adapted for administration by topical route.

[0116] Thus, said composition may include all constituents usually employed in the envisaged topical application and administration.

[0117] Said composition may advantageously be in the form of an emulsion, in particular obtained by dispersing an aqueous phase in an oily phase (W / O) or an oily phase in an aqueous phase (W / O), of liquid or semi-liquid consistency. Liquid of the milky type, or of soft consistency, or even of multiple emulsion (W / O / W or W / O / O). These compositions are prepared according to known usual methods.

[0118] More specifically, said composition may be intended for topical application and preferably may be in the form of an emulsion, preferably an oil-in-water emulsion. Preferably, such an emulsion is not intended to be rinsed off after application.

[0119] Said composition is preferentially intended to be applied to keratinous materials, preferably the skin.

[0120] Said composition according to the invention can be manufactured by any known process generally used in the cosmetic field.

[0121] The ingredients are mixed before shaping, in the order and under conditions easily determined by those skilled in the art.

[0122] According to a particular embodiment of the invention, other agents intended to enhance the appearance and / or texture of the skin may also be added to said composition.

[0123] The present invention further relates to a non-therapeutic cosmetic treatment method for keratinous materials, preferably skin, comprising the application, preferably topically, to keratinous materials, preferably skin, of a composition comprising in a physiologically acceptable medium an extract according to the invention, to promote and / or accelerate the healing and / or repair and / or regeneration of keratinous materials, preferably skin.

[0124] The cosmetic uses and processes considered according to the invention are non-therapeutic.

[0125] The cosmetic uses and processes of the invention are preferably implemented by topically administering an extract according to the invention or a composition according to the invention.

[0126] By way of illustration, the cosmetic use or process according to the invention can be implemented by topical application, for example daily, of at least one composition according to the invention, which can be, for example, formulated as a cream, gel, serum, lotion, emulsion or cleansing milk, preferably in the form of an emulsion.

[0127] The application can be repeated for example 1 to 2 times daily over a day or more and generally over a prolonged period of at least 4, or even 4 to 15 weeks, with where appropriate one or more periods of interruption.

[0128] According to one embodiment, the application is daily (once a day) and generally over a prolonged period of at least 4, or even 4 to 15 weeks, with, where appropriate, one or more periods of interruption.

[0129] According to one embodiment, the cosmetic treatment process according to the invention may comprise a single application.

[0130] The present invention also relates to a method for preparing an extract of at least one bacterium of the species Staphylococcus epidermidis comprising at least one step of culturing at least one bacterium of the species Staphylococcus epidermidis in the presence of a culture medium comprising i) at least one sugar, ii) at least one peptone of vegetable origin and / or at least one yeast extract, and optionally iii) at least one microelement.

[0131] The present invention also relates to an extract of at least one bacterium of the species Staphylococcus epidermidis, preferably an extract of at least one bacterial strain of the species Staphylococcus epidermidis chosen from the group consisting of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694 and 1-5695, capable of being obtained, or obtained, or directly obtained according to the aforementioned preparation process.

[0132] The present invention further relates to a biomass extract of at least one bacterial strain of the species Staphylococcus epidermidis chosen from the group consisting of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694 and 1-5695.

[0133] Advantageously, the steps of the process and the extract are as defined above.

[0134] Throughout the description, including the claims, the expressions "between ... and ..." and "ranging from ... to ..." shall be understood inclusive of bounds, unless otherwise specified.

[0135] The following examples illustrate the present invention without limiting its scope.

[0136] In the examples, unless otherwise indicated, the temperature is ambient temperature (20°C), expressed in degrees Celsius, and the pressure is atmospheric pressure. Examples

[0137] Example 1: Preparation of an extract according to the invention:

[0138] A culture of the Staphylococcus epidermidis CNCM-I 5691 strain is carried out in its complete culture medium in a 10-liter effective bioreactor in batch or fed-batch mode. During this step, the pH is regulated to 6.5 by adding a 1 M KOH solution, the temperature is maintained at 32 °C, and the dissolved oxygen is kept at 40%.

[0139]

[0140] The composition of the initial culture medium is described in Table 2 below. Chemical names Mass concentrations Yeast autolytic extract 5 g / L Soy papain peptone 5 g / L Glucose 6.5 g / L KH2PO4 0.088 g / L CaCl2 0.050 g / L CuSO4, 5 H2O 60 pg / L MnSO4, 1H2O 152 pg / L Kl 20 pg / L ZnSO4, 7H2O 200 pg / L A1C13, 6H2O 100 pg / L water osmosis qsp 1 L

[0142]

[0143] Once the plateau phase is reached after 20 to 48 hours of culture, the cells are extracted and separated by centrifugation (under laboratory conditions, 10,000 g for 30 min). The pellet, also called biomass, containing the cells is then collected, frozen at -20 °C, and subsequently thawed, allowing the cells to rupture and thus yielding a lysate. The lysate is then packaged in bags or tubes and finally stabilized by sterilization at 121 °C for 30 minutes.

[0144] The lysate obtained at the end of the process as described in the example contains 5.2% by weight of dry matter, relative to the total weight of the lysate.

[0145] The analysis of the constituents of the dry extract is presented in Table 3 below.

[0146] [Tables3] Composition (g per 100g of dry extract): Total fat 5.88, Total amino acids 34.30, Total sugars 5.29, Minerals 19.01

[0147] Example 2: Evaluation of keratinocyte migration A. Materials and Methods #

[0148] The model used was described and published in the publication “3D In vitro model of the re-epithelializationphase in the wound-healingprocess” by Nathalie Deshayes, Fabienne Bloas, Florian Boissout, Jennifer Lecardonnel, Maryline Paris, published on June 11, 2017.

[0149] Adapted from the standard 3D TSKIN™ model, the living dermal equivalents (TSKIN™ Lattice, Episkin) are weighted by a metallic ring. Keratinocytes isolated from biopsies are seeded around the ring, leaving the center empty.

[0150] The ring is removed 10 days after keratinocyte seeding. Raw materials to be evaluated for their ability to impact the epidermal regeneration process and the rate of epithelialization are added to the culture medium. An Optical Coherence Tomography (OCT) analysis method combined with a final quantification of histological quality was implemented according to the protocol detailed in the poster "New methodological approach to follow the re-epithelialization phase in the wound-healing process on a 3D full thickness skin model", Fallou Bénédicte, Pelleter Mylène, Innamorato Florence, Roche Mickaël, Bataillon Michel, 25th World Congress of Dermatology, Singapore 2023.

[0151] Optical coherence tomography (OCT) is an optical imaging technique that generates cross-sectional images of tissue structure. OCT uses infrared waves for this purpose. The use of infrared waves allows for much higher resolution (10 mm) at the expense of shallower penetration or imaging depth (2 to 3 mm) (Glinos, GD, et al. (2017). "Optical coherence tomography for assessment of epithelialization in a human ex vivo wound model") Wound Repair Regen. 2017 November; 25(6): 1017-1026. This technique makes it possible to visualize surface features, 2D and 3D structures of the migration pattern during re-epithelialization, and thus to monitor the closure kinetics over 10 days (from day 13 to day 20). Thanks to acquisition and segmentation with specific algorithms, the epidermal and dermal thicknesses as well as the epidermal profile of the tissue after reconstruction are also determined.Next, the overall morphology of the treated tissues is noted at the end of treatment by histology on day 20.

[0152] Each condition was performed in triplicate. A. Results #

[0153]

[0154] The extract of Staphylococcus epidermidis obtained according to Example 1 accelerates epidermal renewal and healing as shown in Table 3 below.

[0155] Indeed, it has been demonstrated that the Staphylococcus epidermidis extract obtained according to Example 1 accelerates keratinocyte migration, leading to faster re-epithelialization. In particular, the extract according to the invention significantly increases (p-value=0.000) the wound closure rate by +11.4% + / -2.59 at day 14. The effect obtained using the extract according to the invention is close to that obtained with the positive reference of the test (Oncostatin M).

[0156] [Tables4] % CLOSING D14 D15 Raw materials tested Effect (vs untreated) Standard deviation p-value Effect (vs untreated) Standard deviation p-value ONCOSTATIN M at 1g / mL in PBS + BSA solvent 0.1% 14.60% 2.67 0.000 9.95% 1.7 0.000 Staphylococcus epidermis extract obtained according to example 1 at 250pg / mL in TSKIN™ culture medium 11.4% 2.59 0.000 7.42% 1.65 0.000

[0157]

Claims

Demands

1. Extract of at least one bacterium of the species Staphylococcus epidermidis obtained by at least one culture step of at least one bacterium of the species Staphylococcus epidermidis, in the presence of a culture medium comprising i) at least one sugar, ii) at least one peptone of vegetable origin and / or at least one yeast extract, and optionally iii) at least one microelement, for its use as a wound-healing agent, and / or for its use in promoting and / or accelerating wound healing and / or repairing and / or regenerating keratinous materials, preferably the skin.

2. Extract for use according to claim 1, characterized in that said culture medium comprises in water: i. at least one sugar present in the culture medium in a concentration of 0.5 to 30 g / L, and ii. at least one peptone of vegetable origin present in the culture medium in a concentration of 0.5 to 15 g / L, and / or at least one yeast extract present in the culture medium in a concentration of 0.5 to 15 g / L, and iii. optionally at least one microelement present in the culture medium in a concentration of 0.05 to 6 mg / L, and iv. optionally at least one calcium salt present in the culture medium in a concentration of 0.01 to 0.1 g / L.

3. Extract for use according to claim 1 or 2, characterized in that said cultured Staphylococcus epidermidis bacterium is a Staphylococcus epidermidis bacterial strain selected from the group of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694 and 1-5695, preferably a Staphylococcus epidermidis bacterial strain selected from the group of bacteria deposited at the CNCM under order numbers 1-5688, 1-5691, 1-5904, 1-5692, 1-5693, 1-5694 and 1-5695, more preferably a Staphylococcus epidermidis bacterial strain epidermidis filed with the CNCM under order number 1-5691.

4. Extract for use according to any one of the preceding claims, characterized in that it comprises, after said culture step, a step of separating the bacteria from the culture medium, preferably by centrifugation, filtration, or coagulation, more preferably by centrifugation.

5. Extract for use according to the preceding claim, characterized in that it comprises after said separation step, a cell lysis step, preferably by osmotic shock, thermal shock, ultrasound, or centrifugation, more preferably by thermal shock.

6. Extract for its use according to the preceding claim, characterized in that it comprises after said cell lysis step, a stabilization step, preferably by autoclaving, ultra-high temperature, high-pressure sterilization, lyophilization, gamma radiation or freezing, preferably by autoclaving.

7. Extract of biomass of at least one bacterial strain of the species Staphylococcus epidermidis selected from the group consisting of bacteria deposited at the CNCM under order numbers 1-5688, 1-5689, 1-5904, 1-5691, 1-5692, 1-5693, 1-5694 and 1-5695, for its use as a wound-healing agent, and / or for its use in promoting and / or accelerating wound healing and / or repair and / or regeneration of keratinous materials, preferably skin.

8. Extract for use according to any one of the preceding claims, wherein said extract is in the form of a lysate.

9. Composition for topical application on keratinous materials, comprising in a physiologically acceptable medium an extract as defined according to any one of claims 1 to 8, for its use in promoting and / or accelerating the healing and / or repair and / or regeneration of keratinous materials, preferably the skin.

10. Composition for its use according to claim 9, wherein said extract is present in the composition in a content of 0.0001% to 20% by weight of dry matter relative to the total weight of the composition, preferably in a content of 0.001% to 10% by weight of dry matter, more preferably in a content of 0.01% to 5% by weight of dry matter, even better in a content of 0.1% to 1% by weight of dry matter relative to the total weight of the composition.