Dietary supplement composition

Abstract

A dietary supplement composition in the form of a carbonated beverage comprising a concentrate of red cherry, an extract of Goji berry, magnesium, and tryptophan, and comprising at least 0.5mg of phyt

Description

The present invention relates to a dietary supplement composition which contains one or more natural sources of melatonin. The dietary supplement composition of the present invention is a liquid formulation. It is prepared as a beverage in the form of a carbonated liquid. It is well known that the hormone melatonin regulates night and day cycles and therefore plays a role in sleep-wake cycles. Synthetic melatonin is taken in a dietary supplement form in some countries in the world and is recognised as a beneficial agent in sleep disorders and avoiding jet lag. However, synthetic melatonin products are available only on prescription by a medical professional in the UK, Europe, Japan and Australia (for example). The present invention seeks to provide a dietary supplement composition which contains one or more natural sources of melatonin to assist with jet lag and sleep. In the past, dietary supplement compositions containing melatonin have been solid formulations provided in tablet or capsule form, for example. Solid formulations and liquid formulations have very different considerations. For example, ingredients do not interact in dried form but may interact in liquid form prior to consumption. Other considerations include gut absorbability, palatability, dosage, drinkability etc. For these reasons and by way of example, the present inventors have found that the properties of concentrates of red cherry (which has high levels of natural melatonin) are relatively straightforward to include in solid formulations but are problematic in liquid formulations. Also, it is difficult to achieve an effective concentration of natural melatonin in a dietary supplement composition in the form of a beverage. Nevertheless, the present inventors have overcome these problems over several years of development to devise a dietary supplement composition in the form of a liquid formulation that has sufficiently high levels of natural melatonin for providing an effective, over-the-counter beverage product. According to the present invention in one aspect there is provided a dietary supplement composition comprising at least 0.5mg of phytomelatonin per predetermined portion, wherein the dietary supplement composition comprises a concentrate of red cherry, an extract of Goji berry, at least one electrolyte and an amino acid, and wherein the dietary supplement composition is prepared as a beverage in the form of a carbonated liquid. The electrolyte may be magnesium, sodium, potassium or zinc. The electrolyte may be in the form of a salt. The amino acid may be tryptophan. According to the present invention in a second aspect, there is provided a dietary supplement composition comprising a concentrate of red cherry, an extract of Goji berry, magnesium and tryptophan, wherein the composition comprises at least 0.5mg of phytomelatonin per predetermined portion, and wherein the dietary supplement composition is prepared as a beverage in the form of a carbonated liquid. Pursuant to the invention it has been found that providing such a combination of ingredients in a carbonated beverage can give rapid uptake and be stable and pleasant tasting, while providing an effective amount of natural melatonin. In this respect, phytomelatonin is the term used for melatonin (N-acetyl-5-methoxytryptamine) of plant origin. In one embodiment, the composition comprises at least 1.0 mg of phytomelatonin per predetermined portion. The composition is administered orally to assist sleep and / or to reduce jet lag. The phytomelatonin in the composition is natural, meaning that the melatonin is derived from natural source(s). The composition preferably contains no synthetic melatonin. The red cherry concentrate is preferably a concentrate of a sour cherry such as a Montmorency cherry. Any red cherry that has a relatively high level of melatonin may be used. The red cherry concentrate may be a concentrated juice. The magnesium may be a magnesium salt such as Magnesium threonate. Magnesium L-threonate and trimagnesium dicitrate are preferred magnesium salts. The tryptophan is preferably L-tryptophan. The inventors have surprisingly been able to create a dietary supplement composition prepared as a beverage in the form of a carbonated liquid which has sufficiently high levels of phytomelatonin to provide effective products for reducing jet lag and / or improving sleep while being palatable for users. By providing a carbonated beverage, the uptake and absorption of melatonin is significantly quickened. A unique blend of four key ingredients is used in a carbonated liquid beverage. The present inventors have devised the beverage despite the knowledge that red cherry concentrate is unpalatable in liquid form and is generally not suitable for drinking, for example in terms of viscosity and foaming. The provision of these ingredients in a carbonated liquid addresses these problems, with the carbonation acting to modify the adverse effects of the red cherry concentrate in liquid form. A red cherry such as Montmorency cherry is provided in concentrate form together with Goji berry extract and magnesium. Goji berry is also a natural source of melatonin: it contains high concentrations of melatonin relative to common dried fruit such as raisins. Magnesium has many beneficial effects including contributing to a reduction of tiredness and fatigue and a normal functioning of the nervous system. It may be used to improve relaxation. Gamma-aminobutyric acid (GABA) is a neurotransmitter in the brain that calms the nervous system by stopping certain signals from transmitting. Magnesium may play a role in regulating GABA, which could help promote calm and act as a sedative, to promote sleep. Magnesium may also inhibit another neurotransmitter, called the N-methyl-D-aspartate receptor, which could promote muscle relaxation. This could help improve general relaxation and sleep. The dietary supplement composition also includes tryptophan which is a precursor of melatonin and of the neurotransmitter serotonin, and is known to increase brain serotonin and melatonin levels. It is understood that the beneficial effects of tryptophan will increase overtime if it is ingested on a regular basis. Tryptophan has a direct synergy with the other ingredients in the composition that release natural melatonin. The dietary supplement composition may comprise sour cherry juice extract as a further source of melatonin. This juice extract may be obtained from a sour cherry such as Montmorency cherry. The dietary supplement composition may comprise lemon balm extract and / or Ashwaganda extract and / or Valerian root and / or Chamomile flower extract. Lemon balm is an herb from the mint family known for its calming effects and ability to reduce anxiety and stress. Ashwagandha is an adaptogenic herb known for its ability to reduce stress and anxiety, which can indirectly promote better sleep. The root of the herb Valerian has a long history of use as a sedative. Chamomile contains apigenin, an antioxidant that binds to certain brain receptors that promote sleepiness and reduce insomnia. The dietary supplement composition may comprise pineapple flavouring which contains melatonin as well as serotonin which can be converted into melatonin. These ingredients interact in various ways to support melatonin production and enhance sleep quality. Some, like Montmorency cherry and pineapple, directly provide melatonin; others are precursors to melatonin (eg tryptophan); while others, such as ashwagandha, chamomile, and lemon balm, promote relaxation and reduce stress, which can enhance the effectiveness of melatonin in regulating sleep. In a preferred embodiment, the dietary supplement composition comprises Montmorency cherry concentrate, Goji berry extract, magnesium citrate, tryptophan, Ashwagandha extract, Chamomile Flower extract, lemon balm extract, Valerian root and pineapple flavouring. The dietary supplement composition therefore contains serotonin direct impactors (eg Montmorency cherry, pineapple), precursors which lead up to melatonin release (eg tryptophan) and other ingredients which assist and boost the effects of melatonin (eg chamomile flower extract, lemon balm extract. Valerian root, Ashwagandha extract). The dietary supplement composition may comprise collagen which may be in the form of collagen peptides, particularly hydrolysed collagen peptides. Collagen peptides are beneficial to enhance recovery from exercise, for example, and thus may assist with healthy sleep patterns. Collagen may also be beneficial to skin health. The levels of melatonin in the dietary supplement composition of the present invention establish whether the composition is suitable for reducing jet lag or for assisting sleep. In this regard, when the composition comprises at least 0.5 mg of melatonin per predetermined portion, it is permissible to make a health claim under EU Commission Regulation No 432 / 2012 that the melatonin contributes to the alleviation of subjective feelings of jet lag. According to the Regulation, the claim may be used only for food which contains at least 0.5 mg of melatonin per quantified portion. In order to bear the claim, information shall be given to the consumer that the beneficial effect is obtained with a minimum intake of 0.5 mg to be taken close to bedtime on the first day of travel and on the following few days after arrival at the destination When the composition comprises at least 1.0 mg of melatonin per predetermined portion, it is permissible to make a health claim under EU Commission Regulation No 432 / 2012 that the melatonin contributes to the reduction of time taken to fall asleep. According to the Regulation, the claim may be used only for food which contains 1 mg of melatonin per quantified portion. In order to bear the claim, information shall be given to the consumer that the beneficial effect is obtained by consuming 1 mg of melatonin close to bedtime. The dietary supplement composition of the present invention may be used for adults or children. In one example, the dietary supplement composition is formulated for children to assist with sleep and may assist with Delayed Sleep-Wake Disorder (DSWPD). in an embodiment of the present invention, the concentrate of red cherry, the extract of Goji berry, the magnesium and the tryptophan are in the relative proportions of: 25 to 120 grams, preferably 25 to 110 grams of red cherry concentrate; 0.3 to 1.5 grams, preferably 0.3 to 1.4 grams of Goji berry extract; 0.3 to 1.6 grams, preferably 0.4 to 1.5 grams of a magnesium salt; and 0.1 to 0.6 grams, preferably 0.1 to 0.5 grams of tryptophan per predetermined portion. The dietary supplement composition may comprise the relative proportions of 0.6 to 3.0 grams, preferably 0.7 to 2.5 grams of sour cherry juice extract per predetermined portion. The dietary supplement composition may comprise relative proportions of 0.1 to 0.6 grams, preferably 0.1 to 0.5 grams of lemon balm extract per predetermined portion. The dietary supplement composition may comprise relative proportions of 0.1 to 0.6 grams, preferably 0.2 to 0.5 grams of pineapple flavouring per predetermined portion. When the composition comprises at least 0.5 mg of phytomelatonin per predetermined portion and less than 1.0 mg of phytomelatonin per predetermined portion, the concentrate of red cherry, the extract of Goji berry, the magnesium and the tryptophan are preferably in the relative proportions of: 25 to 70 grams, preferably 25 to 55 grams of red cherry concentrate; 0.3 to 0.7 grams of Goji berry extract; 0.3 to 1.0 grams, preferably 0.4 to 0.9 grams of a magnesium salt; and 0.1 to 0.3 grams, preferably 0.1 to 0.25 grams of tryptophan per predetermined portion. The dietary supplement composition may comprise relative proportions of 0.6 to 1.3 grams of sour cherry juice extract per predetermined portion. The dietary supplement composition may comprise relative proportions of 0.1 to 0.3 grams, preferably 0.1 to 0.25 grams of lemon balm extract per predetermined portion. When the composition comprises at least 1.0 mg of phytomelatonin per predetermined portion, the concentrate of red cherry, the extract of Goji berry, the magnesium and the tryptophan are preferably in the relative proportions of: 80 to 120 grams, preferably 90 to 110 grams of red cherry concentrate; 1.0 to 1.5 grams, preferably 1.2 to 1.4 grams of Goji berry extract; 1.2 to 1.6 grams, preferably 1.3 to 1.5 grams of a magnesium salt; and 0.2 to 0.6 grams, preferably 0.3 to 0.5 grams of tryptophan per predetermined portion. The dietary supplement composition may comprise relative proportions of 1.0 to 3.0 grams, preferably 1.5 to 2.5 grams of sour cherry juice extract per predetermined portion. The dietary supplement composition may comprise relative proportions of 0.2 to 0.6 grams, preferably 0.3 to 0.5 grams of lemon balm extract per predetermined portion. The dietary supplement composition may comprise: 10 to 40 wt%, preferably 12 to 38 wt% of red cherry concentrate, 0.20 to 0.40 wt%, preferably 0.20 to 0.35 wt% of Goji berry extract, 0.15 to 0.50 wt%, preferably 0.20 to 0.45 wt% of magnesium salt and 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of tryptophan, of the total liquid weight. The dietary supplement composition may comprise 0.40 to 0.60 wt%, preferably 0.45 to 0.55 wt% of sour cherry juice extract of the total liquid weight. The dietary supplement composition may comprise 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of lemon balm extract of the total liquid weight. The dietary supplement composition may comprise 0.10 to 0.30 wt%, preferably 0.10 to 0.20 wt% of pineapple flavouring of the total liquid weight. The dietary supplement composition, being in liquid form, imay be packaged in predetermined portions of 150ml to 500ml and preferably in predetermined portions of 150ml, 250ml or 400ml. A 250ml portion is particularly preferred. The dietary supplement composition may comprise one or more sweeteners; and / or one or more flavourings; and / or one or more flavour modifiers; and / or one or more surface active agents; and / or one of more foam control agents. The dietary supplement composition may be flavoured using any known flavours, particularly natural flavours. In one embodiment, the dietary supplement composition comprises a peach flavouring. In another embodiment, the dietary supplement composition comprises a pineapple flavouring. The pineapple flavouring is preferably a 100% natural fruit flavouring made from high quality raw materials. Preferably, it does not contain fermentable sugars. The dietary supplement composition may comprise: 10 to 40 wt%, preferably 12 to 38 wt% of red cherry concentrate, 0.20 to 0.40 wt%, preferably 0.20 to 0.35 wt% of Goji berry extract, 0.15 to 0.50 wt%, preferably 0.20 to 0.45 wt% of magnesium salt and 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of tryptophan, optionally 0.40 to 0.60 wt%, preferably 0.45 to 0.55 wt% of sour cherry juice extract, optionally 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of lemon balm extract, optionally 0.01 to 0.50 wt%, preferably 0.01 to 0.03 wt% of one or more foam control agents, optionally 0.15 to 0.40 wt%, preferably 0.20 to 0.30 wt% of one or more non-magnesium minerals, optionally 0.10 to 0.40 wt%, preferably 0.12 to 0.30 wt% of one or more flavourings, optionally 0.03 to 0.12 wt%, preferably 0.05 to 0.09 wt% of one or more sweeteners, and optionally 0.05 to 0.30 wt%, preferably 0.10 to 0.20 wt% of one or more flavour modifiers of the total liquid weight In one embodiment, when the composition comprises at least 0.5 mg of phytomelatonin per predetermined portion and less than 1.0 mg of phytomelatonin per predetermined portion, the liquid supplement composition may comprise: 25 to 40 wt%, preferably 30 to 35 wt% of red cherry concentrate, 0.20 to 0.40 wt%. preferably 0.20 to 0.30 wt% of Goji berry extract, 0.25 to 0.40 wt%, preferably 0.25 to 0.35 wt% of magnesium salt and 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of tryptophan, optionally 0.40 to 0.60 wt%, preferably 0.45 to 0.55 wt% of sour cherry juice extract, optionally 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of lemon balm extract, optionally 0.01 to 0.04 wt%, preferably 0.01 to 0.03 wt% of one or more foam control agents, optionally 0.15 to 0.40 wt%, preferably 0.20 to 0.30 wt% of one or more non-magnesium minerals, optionally 0.15 to 0.40 wt%, preferably 0.20 to 0.30 wt% of one or more flavourings, optionally 0.03 to 0.12 wt%, preferably 0.05 to 0.09 wt% of one or more sweeteners, and optionally 0.05 to 0.30 wt%, preferably 0.10 to 0.20 wt% of one or more flavour modifiers of the total liquid weight. in this embodiment, the composition may comprise: 30 to 38 wt%, preferably about 34 wt% of red cherry concentrate, 0.2.2 to 0.28 wt%, preferably about 0.25 wt% of Goji berry extract, 0.29 to 0.35 wt%, preferably about 0.32 wt% of magnesium salt and 0.08 to 0.12 wt%, preferably about 0.10 wt% of tryptophan, of the total liquid weight. The predetermined portion may be 150ml. In another embodiment, when the composition comprises at least 0.5 mg of phytomelatonin per predetermined portion and less than 1.0 mg of phytomelatonin per predetermined portion, the liquid supplement composition may comprise: 10 to 25 wt%, preferably 10 to 20 wt% of red cherry concentrate, 0.20 to 0.40 wt%, preferably 0.20 to 0.30 wt% of Goji berry extract, 0.35 to 0.50 wt%, preferably 0.40 to 0.45 wt% of magnesium salt and 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of tryptophan, optionally 0.40 to 0.60 wt%, preferably 0.45 to 0.55 wt% of sour cherry juice extract, optionally 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of lemon balm extract, optionally 0.01 to 0.04 wt%, preferably 0.01 to 0.03 wt% of one or more foam control agents, optionally 0.15 to 0.40 wt%, preferably 0.20 to 0.30 wt% of one or more non-magnesium minerals, optionally 0.15 to 0.40 wt%, preferably 0.20 to 0.30 wt% of one or more flavourings, optionally 0.03 to 0.12 wt%, preferably 0.05 to 0.09 wt% of one or more sweeteners, and optionally 0.05 to 0.30 wt%, preferably 0.10 to 0.20 wt% of one or more flavour modifiers of the total liquid weight. In this embodiment, the composition may comprise: 14 to 16 wt%, preferably about 15 wt% of red cherry concentrate, 0.22 to 0.28 wt%, preferably about 0.25 wt% of Goji berry extract, 0.40 to 0.45 wt%, preferably about 0.43 wt% of magnesium salt and 0.08 to 0.12 wt%, preferably about 0.10 wt% of tryptophan, of the total liquid weight. The predetermined portion may be 2.50mL in another embodiment when the composition comprises at least 0.5 mg of phytomelatonin per predetermined portion and less than 1.0 mg of phytomelatonin per predetermined portion, the liquid supplement composition may comprise: 10 to 25 wt%, preferably 10 to 20 wt% of red cherry concentrate, 0.20 to 0.40 wt%, preferably 0.20 to 0.30 wt% of Goji berry extract, 0.10 to 0.30 wt%, preferably 0.15 to 0.25 wt% of magnesium salt and 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of tryptophan, optionally 0.40 to 0.60 wt%, preferably 0.45 to 0.55 wt% of sour cherry juice extract, optionally 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of lemon balm extract, optionally 0.40 to 1.00 wt%, preferably 0.50 to 0.90 wt% of Chamomile Flower extract, optionally 0.10 to 0.30 wt%, preferably 0.15 to 0.25 wt% of Ashwaganda extract, optionally 0.10 to 0.30 wt%, preferably 0.15 to 0.25 wt% of Valerian root, optionally 0.01 to 0.04 wt%, preferably 0.01 to 0.03 wt% of one or more foam control agents, optionally 0.10 to 0.30 wt%, preferably 0.10 to 0.20 wt% of pineapple flavouring, optionally 0.03 to 0.12 wt%, preferably 0.05 to 0.09 wt% of one or more sweeteners, and optionally 0.05 to 0.30 wt%, preferably 0.10 to 0.20 wt% of one or more flavour modifiers of the total liquid weight. In this embodiment, the composition may comprise: 14 to 16 wt%, preferably about 15 wt% of red cherry concentrate, 0.22 to 0.28 wt%, preferably about 0.25 wt% of Goji berry extract, 0.18 to 0.25 wt%, preferably about 0.21 wt% of magnesium salt and 0.08 to 0.12 wt%, preferably about 0.10 wt% of tryptophan, of the total liquid weight. The predetermined portion may be 250ml. When the composition comprises at least 1.0 mg of phytomelatonin per predetermined portion, the liquid supplement composition may comprise: 2,0 to 35 wt%, preferably 20 to 30 wt% of red cherry concentrate, 0.20 to 0.40 wt%, preferably 0.30 to 0.35 wt% of Goji berry extract, 0.25 to 0.45 wt%, preferably 0.30 to 0.40 wt% of magnesium salt and 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of tryptophan, optionally 0.40 to 0.60 wt%, preferably 0.45 to 0.55 wt% of sour cherry juice extract, optionally 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of lemon balm extract, optionally 0.01 to 0.04 wt%, preferably 0.01 to 0.03 wt% of one or more foam control agents, optionally 0.15 to 0.40 wt%, preferably 0.30 to 0.40 wt% of one or more non-magnesium minerals, optionally 0.15 to 0.40 wt%, preferably 0.20 to 0.30 wt% of one or more flavourings, optionally 0.03 to 0.12. wt%, preferably 0.05 to 0.09 wt% of one or more sweeteners, and optionally 0.05 to 0.30 wt%, preferably 0.10 to 0.20 wt% of one or more flavour modifiers of the total liquid weight. In this embodiment, the composition may comprise: 22 to 28 wt%, preferably about 25 wt% of red cherry concentrate, 0.29 to 0.35 wt%, preferably about 0.32 wt% of Goji berry extract, 0.40 to 0.40 wt%, preferably about 0.35 wt% of magnesium salt and 0.08 to 0.12 wt%, preferably about 0.10 wt% of tryptophan, of the total liquid weight. The predetermined portion may be 400ml. in the present invention, the red cherry concentrate may have a value of degree Brix of at least 50, preferably of at least 60. Degree Brix is a calibration of the refractive index of a solution against dissolved sucrose, it is therefore a measure of the dissolved solids in a liquid, and is commonly used to measure dissolved sugar content of an aqueous solution. One degree Brix is 1 gram of sucrose in 100 grams of solution and represents the strength of the solution as percentage by mass. The present invention also relates to a method of manufacturing a dietary supplement composition, an oral dose formulation of a food for dietary supplement and a method of improving sleep quality or alleviating the symptoms of jet lag comprising administering to a subject a dietary supplement composition. The invention wil! now be described, by way of example only, with reference to the accompanying figure. A dietary supplement composition according to an embodiment of the present invention is a 150ml carbonated beverage having the following composition containing at least 0.5mg of phytomelatonin. INGREDIENT Filtered Water 1 QTY grams | PER LITRE | remainder QTY grams PER BOTTLE remainder % of Unit Total remainder Red Cherry Concentrate | 339 50.850 33.90% Sour Cherry Juice Extract 1 5 0.750 0.50% Magnesium Salt | 3.2424 0.486 0.32% Goji Berry Extract | 2.5 0.375 0.25% Non-magnesium mineral | 2.5 0.375 0.25% Flavouring | 2.2 0.330 0.22% Trytophan 1 1 0.150 0.10% Lemon Balm Extract 1 1 0.150 0.10% Flavour modifier 1 1 1 0.150 0.10% Sweetener | 0.65 0.098 0.07% Flavour modifier 2 | 0.5 0.075 0.05% Foam Control Agent | 0.15 0.023 0.02% 5 A dietary supplement composition according to an embodiment of the present invention is a 250ml carbonated beverage having the following composition containing at least O.Smg of phytomelatonin. INGREDIENT QTY grams PER LITRE QTY grams PER BOTTLE % of Unit Total Filtered Water remainder remainder remainder Red Cherry Concentrate i53^ 30J36 15.37% Sour Cherry Juice Extract 1.000 0.50% Magnesium Salt 4.28 0.856 0.43% Goji Berry Extract 2.5 0.500 0.25% Non-magnesium mineral 2.5 0.500 0.25% Flavouring 2.2 0,440 0.22% Tryptophan ^0200 0.10% Lemon Balm Extract 0.200 0.10% Flavour modifier 1 1 0.200 0.10% Sweetener 0.65 0.130 0.07% Flavour modifier 2 0.5 0.100 0.05% Foam Control Agent 11030 0.02% A dietary supplement composition according to another embodiment of the present invention is a 400ml carbonated beverage having the following composition containing at least l.Omg of phytomelatonin. INGREDIENT QTY grams PER LITRE QTY grams PER BOTTLE % of Unit Total Filtered Water remainder remainder remainder Red Cherry Concentrate 253.6 101.440 25.36% Sour Cherry Juice Extract 5 2.000 0.50% Non-magnesium mineral 3.5 1.400 0.35% Magnesium Salt 3.2424 1.297 0.32% Goji Berry Extract 2.5 1.000 0.25% Flavouring 2.2 0.880 0.22% Tryptophan 1 0.400 0.10% Lemon Balm Extract 1 0.400 0.10% Flavour modifier 1 i 6.400 6.16% Sweetener 0.8 0.320 0.08% Flavour modifier 2 0.5 0.200 0.05% Foam control agent 0.15 0.060 0.02% A dietary supplement composition according to an embodiment of the present invention is a 250ml carbonated beverage having the following composition containing at least 0.5mg of phytomelatonin. INGREDIENT | QTY grams | PER LITRE QTY grams PER BOTTLE % of Unit total Filtered Water 1 remainder remainder remainder Red Cherry Concentrate 153.68 38.420 15.37 Chamomile Flower Extract 6.668 1.667 0.67 Sour Cherry Juice Extract 5 1.250 0.50 Goji Berry Extract 77i 71625 7125 Magnesium Salt 2.14 0.535 0.21 Ashwaganda Extract 2 0.500 0.20 Valerian Root 2 0.500 0.20 Pineapple Flavouring Ts 71375 7115 Tryptophan 1 0.250 0.10 Lemon Balm Extract 1 0.250 0.10 Flavour modifier 1 1 0.250 0.10 Sweetener 0.65 0.163 0.07 Flavour modifier 2 0.5 0.125 0.05 Foam control agent 0.15 0.038 0.02 These ingredients may be sourced from the following suppliers in the UK. ingredient details Sour Cherry Juice Extract UK Supplier 1 Blue Sky Botanies Ltd | Product code ESRCHR002 | Goji Berry (Org) Extract Blue Sky Botanies Ltd [ 20E 1:2.5 Product code EGOJ1003 | Lemon Balm Extract Blue Sky Botanies Ltd | 20EpH 1:2.5 Product code ELBALM001 | Red Cherry Concentrate Gerald McDonald &Co Ltd 1 65 Brix Clarified Product code 3421.05 | Flavouring: IMCD UK Ltd | Peach Flavour Product code AO-696-755-0 | Flavouring: Kerry Ingredients &Flavours Ltd | Pineapple Flavour Product code 20123873 | Foam control agent: Brenntag SE Product code F2010 1 FoamDoctor' Flavour modifier 1: Kerry Ingredients &Flavours Ltd I TasteSense"”' Masking Flv Product code 20497883 | Flavour modifier 2: Kerry Ingredients &Flavours Ltd | TasteSenseTM SR LQD 100714089 Product code 20465384 1 Non-magnesium mineral Nutraceuticals Group Europe Ltd 1 Trisodium Citrate Dihydrate Nutrition Grade Product code NIGEMIN003402 | “34% Na) Magnesium Salt Nutraceuticals Group Europe Ltd j Magnesium Threonate Nutrition Grade (~7% Product code NIGEMIN002425 or | Mg) or Nutraceuticals Group Europe Ltd | Magnesium Citrate Tribasic Anhydrous Product code NIGEMIN002101 | Nutrition Grade Granular 30 Mesh (-14% Mg) L-Tryptophan Vegan Fermented Nutraceuticals Group Europe Ltd j Product code NIGEAMA006901 | Chamomile Flower Extract 3% Apigenin Nutraceuticals Group Europe Ltd | (Mlatricaria recutita / chamomilla) Product code NIGEHER009903 | Ashwaganda (Org) Extract 20EX 1:5 Blue Sky Botanies Ltd | Product code EASHW001 | Valerian Root Hydrolat 80H20E BSB10 Blue Sky Botanies Ltd | । i Sweetener: Nutraceuticals Group Europe Ltd | Stevia Extract Rebaudioside A 97% (Stevia Product code NIGESWE00701 | rebaudiana) The Red Cherry Concentrate may be 65 Brix Clarified and produced using the steps set out in the diagram of figure 1. The red cherry fruit, once screened for quality, is crushed. It is then heated and cooled before being pressed to extract raw juice which is subsequently concentrated. During a clarification process, pectinases and pectolytic enzymes are degraded and bentonite and fining agents are filtered out. The product is a dark red to purple coloured clear viscous liquid with a flavour and aroma characteristic of red / sour cherry. The Goji Berry extract is made from natural plant material (Lycium barbarum) and the solvent in this ingredient is 20% ethanol (E)). 40 grams of botanical (the fruit) is used to manufacture 100 grams of product. The botanical to solvent ratio is 1:2.5. The solvent in the sour cherry juice extract is 20% ethanol. The lemon balm extract is made from natural plant material (Melissa officinalis) and the solvent in this ingredient is 20% ethanol (E). 40 grams of botanical (the aerial parts) is used to manufacture 100 grams of product. The botanical to solvent ratio is 1:2.5. FoamDoctor® F2010 is a polydimethylsiloxane (silicone, E900 grade) based food safe foam control agent. The flavour modifier TasteSense™ Masking Flv comprises flavouring preparations, natural flavouring substances, water, Glucono deltalactone E575 (20% max), Propylene glycol E1520 (15% max) and Sodium lactate E325 (15% max). The flavour modifier Flavour TasteSenseTM SR LQD 100714089 comprises flavouring preparations, natural flavouring substances, water, Propylene glycol E1520 (33% max) and Maltodextrin. The ingredients listed above provide a good flavour profile for the beverage which has a cherry and peach flavour. The dietary supplement composition is not to limited to these specific ingredients or to this flavour. In other embodiments, the dietary supplement composition has different fruit flavours combined with the cherry flavour. Examples are cherry and pineapple, cherry and apple, cherry and orange, cherry and mango, cherry and tropical fruit. The dietary supplement composition may also have other flavours combined with the cherry flavour. Examples Test Reports: Phytomelatonin Analysis Cherry and Peach 150ml beverage - jet lag claim Sample 1 416ng phytomelatonin / lOOg Sample 2 409ng phytomelatonin / lOOg Sample 3 422ng phytomelatonin / lOOg Cherry and Peach 400ml beverage - jet lag clai Sample 1 466ng phytomelatonin / lOOg Sample 2 472ng phytomelatonin / lOOg Cherry and Peach 250ml beverage - sleep claim Sample 1 502ng phyto mel aton i n / 100g Sample 2 499ng phytomelatonin / lOOg Sample 3 505ng phytomelatonin / lOOg Cherry and Peach 400ml beverage - sleep claim Sample 1 465ng phytomelatonin / lOOg

Claims

1. A dietary supplement composition comprising a concentrate of red cherry, an extract of Goji berry, magnesium and tryptophan, wherein the composition comprises at least 0.5mg of phytomelatonin per predetermined portion, and wherein the dietary supplement liquid composition is in the form of a carbonated beverage.

2. A dietary supplement composition as claimed in claim 1, wherein the composition comprises at least 1.0 mg of phytomelatonin per predetermined portion.

3. A dietary supplement composition as claimed in claim 1 or claim 2, wherein the red cherry concentrate is a concentrate of a sour cherry, preferably Montmorency cherry.

4. A dietary supplement composition as claimed in any preceding claim, further comprising one or more ingredients selected from the group consisting of: sour cherry juice extract, pineapple flavouring, lemon balm extract, Ashwagandha extract, Chamomile Flower extract, Valerian root and collagen.

5. A dietary supplement composition as claimed in any preceding claim, wherein the magnesium is a magnesium salt and the concentrate of red cherry, the extract of Goji berry, the magnesium and the tryptophan are in the relative proportions of: 25 to 120 grams, preferably 25 to 110 grams of red cherry concentrate;0.3 to 1.5 grams, preferably 0.3 to 1.4 grams of Goji berry extract;0.3 to 1.6 grams, preferably 0.4 to 1.5 grams of the magnesium salt; and0.1 to 0.6 grams, preferably 0.1 to 0.5 grams of tryptophan per predetermined portion.

6. A dietary supplement composition as claimed in claim 5 when dependent on claim 4, further comprising the relative proportions of 0.6 to 3.0 grams, preferably 0.7 to 2.5 grams of sour cherry juice extract and / or the relative proportions of 0.1 to 0.6 grams, preferably 0.1 to 0.5 grams of lemon balm extract per predetermined portion and / or the relative proportions of 0.1 to 0.6 grams, preferably 0.2 to 0.5 grams of pineapple flavouring per predetermined portion.

7. A dietary supplement composition as claimed in any preceding claim comprising:10 to 40 wt%, preferably 12. to 38 wt% of red cherry concentrate, 0.20 to 0.40 wt%, preferably 0.20 to 0.35 wt% of Goji berry extract, 0.15 to 0.50 wt%, preferably 0.2.0 to 0.45 wt% of magnesium salt and 0.05 to 0.2.0 wt%, preferably 0.05 to 0.15 wt% of tryptophan, of the total liquid weight of the composition.

8. A dietary supplement composition as claimed in claim 7 when dependent on claims 4 to 6, further comprising 0.40 to 0.60 wt%, preferably 0.45 to 0.55 wt% of sour cherry juice extract and / or 0.05 to 0.20 wt%, preferably 0.05 to 0.15 wt% of lemon balm extract and / or 0.10 to 0.30 wt%, preferably 0.10 to 0.20 wt% of pineapple flavouring of the total liquid weight of the composition.

9. A dietary supplement composition as claimed in any preceding claim, further comprising one or more sweeteners; and / or one or more flavourings; and / or one or more flavour modifiers; and / or one or more surface active agents; and / or one or more foam control agents.

10. A method of manufacturing a dietary supplement composition comprising admixing a concentrate of red cherry, an extract of Goji berry, magnesium and tryptophan, wherein the composition is the dietary supplement composition of any preceding claim.

11. An oral dose formulation of a food for dietary supplement according to any one of ciaims 1 to 9, being a liquid formulation.

12. A method of improving sleep quality or alleviating the symptoms of jet lag comprising administering to a subject a dietary supplement composition according to any one of claims 1 to 9.

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