Skin darkening improver

Abstract

To provide a loricrin expression promoter, a horny layer formation promoter and a skin darkening improver which promote loricrin expression.SOLUTION: A loricrin expression promoter contains Eucalyptus globulus or its extract, and Prunus persica or its extract as active ingredients.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a loricrin expression promoter, a stratum corneum formation promoter, and an agent for improving skin dullness. [Background technology]

[0002] In order to improve the skin's ability to retain moisture, prevent and improve rough skin, and prevent and improve skin aging such as the formation of wrinkles and the reduction of texture, it is believed that it is effective to act on the epidermal cells of the skin, promote the keratinization of the epidermal cells, promote the formation of a healthy stratum corneum, and improve the stratum corneum barrier function to protect the body from external stimuli and other factors. In addition, the formation of a healthy stratum corneum also leads to the improvement of dull skin (Non-Patent Document 1). Dull skin is a phenomenon that occurs on the entire face or on areas such as around the eyes and cheeks, in which the skin's transparency decreases and it appears dark, with unclear boundaries. There are several thought to be factors that cause dull skin, one of which is a decrease in transparency (light transmittance) due to thickening of the stratum corneum (Non-Patent Document 2).

[0003] Thickening of the stratum corneum refers to a state in which the stratum corneum is thicker than normal, and is also called stratum corneum stratification. The cause of thickening of the stratum corneum is thought to be the disruption of turnover due to the effects of aging, dryness, ultraviolet rays, etc., which causes abnormalities in the formation and peeling of stratum corneum cells. Turnover here refers to the continuous repetition of the proliferation of keratinocytes (epidermal keratinocytes) in the basal layer, the process of keratinization, and the peeling of the stratum corneum. During the keratinization process, these keratinocytes form each layer, changing sequentially from basal cells, spinous cells, granular cells, and stratum corneum cells toward the outside. Then, proteins that make up the cornified envelope (CE) are synthesized from the upper spinous layer to the granular layer. Furthermore, in the process of reaching the stratum corneum, matrix proteins such as involucrin, loricrin, and cystatin are bound to the cell membrane of keratinocytes by the enzyme transglutaminase, forming insoluble CE. Furthermore, ceramide and other substances are covalently bound to the insoluble CE, forming the basis of the stratum corneum barrier function. Eventually, as they reach the surface layer of the stratum corneum, the adhesive strength of the keratinocytes weakens and they become scum and fall off. Therefore, the abnormal formation and peeling of the stratum corneum in the turnover disorder leads to abnormal parakeratinization, which is one of the causes of thickening of the stratum corneum. Conventionally, skin troubles caused by the abnormal turnover disorder have been solved by creams containing ceramide. However, the improvement effect of promoting keratinization of epidermal cells and promoting the health of the stratum corneum is not sufficient, so the development of ingredients that promote the formation of a healthy stratum corneum is desired.

[0004] Among the above matrix proteins, loricrin is a major component of CE and is involved in various stratum corneum functions, including the formation of natural moisturizing factors. Diseases caused by loricrin gene mutations are collectively called loricrin keratosis, and symptoms such as diffuse keratinocyte thickening accompanied by abnormal keratinization are observed (Non-Patent Document 3). It has also been reported that transfection of the loricrin gene into HaCaT cells, a cell line of human epidermal keratinocytes, leads to programmed cell death associated with terminal keratinization, and that loricrin is an important molecule for normal keratinization (Non-Patent Document 4). Therefore, it is believed that promoting the expression of loricrin in epidermal cells promotes normal keratinization of keratinocytes and the formation of a healthy stratum corneum, improving stratum corneum thickening, and as a result, improving dull skin. In recent years, loricrin has been attracting attention as a differentiation marker for epidermal cells.

[0005] On the other hand, it has been reported that extracts from Eucalyptus globulus, a member of the Myrtaceae family, have the effect of promoting stratum corneum formation by promoting involucrin expression, and are useful for improving the skin's ability to retain moisture (moisturizing effect) and reducing the visibility of pores (Patent Document 1). In addition, it has been reported that extracts from the peach (Prunus persica) of the Rosaceae family have the effect of promoting involucrin production and are useful in preventing and improving skin conditions such as dry skin, rough skin, atopic dermatitis, and psoriasis by normalizing epidermal keratinocytes (Patent Document 2). However, there have been no reports on the effect of combined use of eucalyptus and peach on loricrin expression. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] JP 2007-277149 A [Patent Document 2] JP 2008-7412 A [Non-patent literature]

[0007] [Non-Patent Document 1] T. Ishida et al. J. Soc. Cosmet. Chem. Jpn. 54 (3): 258-63 (2020) [Non-Patent Document 2] Masako Naganuma Journal of the Japanese Society of Cosmetic Science Vol. 39, No. 4, pp. 275-285 (2015) [Non-Patent Document 3] A. I-Yamamoto. J. Dermatol. Sci. 31 (1): 3-8 (2003) [Non-Patent Document 4] K. Yoneda et al. J. Dermatol. 37 (11): 956-964 (2010) Summary of the Invention [Problem to be solved by the invention]

[0008] The present invention relates to providing a loricrin expression promoter, a stratum corneum formation promoter, and an agent for improving skin dullness, which promote the expression of loricrin. [Means for solving the problem]

[0009] The present inventors have conducted intensive research in light of the above-mentioned problems and have found that combining eucalyptus extract and peach extract synergistically enhances loricrin protein expression, and is useful for promoting stratum corneum formation and improving skin dullness.

[0010] That is, the present invention relates to the following 1) to 3). 1) A loricrin expression promoter having eucalyptus or its extract, and peach or its extract as active ingredients. 2) A stratum corneum formation promoter containing eucalyptus or its extract, and peach or its extract as active ingredients. 3) A skin dullness improving agent containing eucalyptus or its extract, and peach or its extract as active ingredients. Effect of the Invention

[0011] According to the present invention, by promoting the expression of loricrin, it is possible to promote the formation of a healthy stratum corneum and improve dull skin. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] In this specification, "eucalyptus" refers to Eucalyptus globulus, which belongs to the genus Eucalyptus in the family Myrtaceae. "Peach" refers to the peach (Prunus persica) of the Rosaceae family. Examples of the parts of the plants that can be used include the whole plant, the whole tree, the leaves, the stems, the shoots, the flowers, the buds, the trees, the woody parts, the bark, the roots, the rhizomes, the pseudocorms, the tuberous roots, the lichens, the thallus, the seeds, the fruits, the resins, the kernels, and mixtures thereof. In the case of eucalyptus, the parts of the plants that can be used are preferably the leaves, the flowers, the fruits, and more preferably the leaves. In the case of peach, the parts of the plants that can be used are preferably the leaves, the flowers, the fruits, and the kernels, and more preferably the leaves.

[0013] Such plants can be used as they are or as juice obtained by squeezing them, as a dried product obtained by drying the plant body itself or its pulverized product, or as an extract extracted from these, but it is preferable to use them as an extract.

[0014] Examples of the extract include various solvent extracts obtained by extracting the above-mentioned plants at room temperature or under heating, or by using an extraction device such as a Soxhlet extractor, extracts obtained by supercritical extraction using supercritical carbon dioxide or the like, dilutions thereof, concentrates thereof, and dried powders thereof. The extraction means is not particularly limited, but may be any of the usual means such as soaking, decoction, percolation, reflux extraction, ultrasonic extraction, microwave extraction, stirring, etc.

[0015] The solvent for extraction may be either a polar solvent or a non-polar solvent. Specific examples of the solvent include water; monohydric, dihydric or polyhydric alcohols; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; linear or cyclic ethers such as diethyl ether and tetrahydrofuran; polyethers such as polyethylene glycol; saturated or unsaturated hydrocarbons such as hexane; aromatic hydrocarbons such as benzene and toluene; halogenated hydrocarbons such as dichloromethane, chloroform, dichloroethane and carbon tetrachloride; pyridines; dimethyl sulfoxide; acetonitrile; carbon dioxide, supercritical carbon dioxide; oils and fats, waxes, other oils; and mixtures thereof. Suitable examples include water, alcohols and aqueous solutions thereof, and examples of alcohols include methanol, ethanol, 1,3-butylene glycol, n-propanol, isopropanol, n-butanol, isobutanol, sec-butanol, t-butanol, etc., and preferably ethanol and 1,3-butylene glycol, and more preferably 1,3-butylene glycol.

[0016] The concentration of the alcohols in the aqueous solution of the alcohols (volume percentage at 25°C, hereinafter referred to as % (v / v)) is preferably 12% (v / v) or more, more preferably 15% (v / v) or more, even more preferably 18% (v / v) or more, and is preferably 99.8% (v / v) or less, more preferably 90% (v / v) or less, even more preferably 85% (v / v) or less. In the case of eucalyptus extract, the concentration of alcohols is preferably 30% (v / v) or more, more preferably 40% (v / v) or more, even more preferably 45% (v / v) or more, and is preferably 99.8% (v / v) or less, more preferably 90% (v / v) or less, even more preferably 85% (v / v) or less. In the case of peach extract, the concentration of alcohols is preferably 12% (v / v) or more, more preferably 15% (v / v) or more, even more preferably 18% (v / v) or more, and is preferably 85% (v / v) or less, more preferably 55% (v / v) or less, even more preferably 45% (v / v) or less.

[0017] The amount of the solvent used in the extraction is preferably 1 to 100 mL, and more preferably 3 to 50 mL, per 1 g of the plant (calculated as a dry mass). The extraction conditions are not particularly limited as long as sufficient extraction can be performed, but for example, the extraction time is preferably 1 hour or more, more preferably 3 hours or more, and is preferably 2 months or less, more preferably 5 weeks or less, and more preferably 3 weeks or less. The extraction temperature is preferably 0° C. or higher, more preferably 5° C. or higher, and is preferably the boiling point of the solvent or lower, more preferably 90° C. or lower. In general, extraction is performed for a long time at a low temperature and for a short time at a high temperature.

[0018] The extract may be a crude product as long as it meets the standards acceptable for cosmetics and pharmaceuticals and exerts the effects of the present invention. If necessary, the extract may be subjected to treatments such as removal of inactive contaminants, deodorization, decolorization, etc., by known techniques such as liquid-liquid distribution, solid-liquid distribution, filtration membrane, activated carbon, adsorption resin, ion exchange resin, and precipitation. The purity of these compounds may be further increased by appropriately combining known separation and purification methods, such as organic solvent precipitation, centrifugation, ultrafiltration membrane, high performance liquid chromatography, and column chromatography.

[0019] The extract may be used as it is, or may be used as a dilution solution diluted with an appropriate solvent, or may be prepared as a concentrated extract, dried powder, or paste. It may also be freeze-dried and diluted with a solvent normally used in extraction, such as water, ethanol, 1,3-butylene glycol, a mixture of water and ethanol, or a mixture of water and 1,3-butylene glycol, before use. It may also be encapsulated in a vesicle such as a liposome or a microcapsule.

[0020] As shown in the Examples below, the combination of eucalyptus extract and peach extract promotes the expression of loricrin. Moreover, the promoting effect is superior to the effect of each of eucalyptus extract and peach extract alone, and exceeds the additive effect when each is added alone, resulting in a synergistic effect. As described above, promoting the expression of loricrin promotes normal keratinocyte keratinization and healthy stratum corneum formation. This is expected to improve dull skin. Therefore, a combination of eucalyptus or an extract thereof and peach or an extract thereof can serve as a loricrin expression promoter, a stratum corneum formation promoter, and an agent for improving skin dullness (hereinafter also referred to as "loricrin expression promoters, etc."), and can be used to promote the expression of loricrin, promote the formation of the stratum corneum, and improve skin dullness, and can also be used to produce loricrin expression promoters, etc. Here, "use" may be for humans or non-human animals, and may be therapeutic or non-therapeutic. "Non-therapeutic" is a concept that does not include medical procedures, i.e., methods of surgery, treatment, or diagnosis of humans, and more specifically, does not include methods of surgery, treatment, or diagnosis performed by a physician, a medical professional, or a person under the instruction of a physician. In the present invention, non-therapeutic use includes the use of the above-mentioned plants or extracts thereof for cosmetic or aesthetic purposes.

[0021] As used herein, "promotion of loricrin expression" encompasses promotion of loricrin expression at the gene level and promotion of loricrin expression at the protein level. Promotion of expression at the gene level includes promotion of transcription into mRNA in the expression of mRNA encoding loricrin, and promotion of expression at the protein level includes promotion of mRNA translation, etc. Of these, promotion of expression at the protein level is preferred in the present invention. Human loricrin is composed of 315 amino acids and has a molecular weight of approximately 26 kDa.

[0022] "Promotion of stratum corneum formation" means that normal keratinization of keratinocytes is promoted, and the formation of a healthy stratum corneum (horny layer) is promoted. Here, a healthy stratum corneum means a stratum corneum with healthy skin turnover, specifically a stratum corneum in which the proliferation of keratinocytes in the basal layer and the peeling of the stratum corneum from the surface layer are balanced, and no thickening of the stratum corneum is observed.

[0023] "Dull skin" refers to a state in which the skin appears dark due to reduced transparency caused by thickening of the stratum corneum, etc. Areas where dull skin occurs include, for example, the entire face, around the eyes, cheeks, forehead, neck, back of the hands, back of the feet, etc. "Improvement" refers to improvement of the condition, prevention or delay of deterioration of the condition, or reversal, prevention or delay of the progression of deterioration of the condition.

[0024] In the present invention, eucalyptus or an extract thereof and peach or an extract thereof may be administered in either order, or they may be administered simultaneously. When the two agents are not administered simultaneously, the administration interval between the two agents may be appropriately selected as long as the effect of enhancing the loricrin expression promoting activity of eucalyptus or an extract thereof, or peach or an extract thereof is exhibited. The agent comprising a combination of eucalyptus or an extract thereof and peach or an extract thereof may be formulated into a single dosage form as a combined preparation, or may be a kit in which the individual preparations can be used simultaneously or separately at intervals.

[0025] The loricrin expression promoter etc. of the present invention may be a drug, quasi-drug, or cosmetic itself for promoting the expression of loricrin, promoting the formation of the stratum corneum, or improving dullness of the skin, or may be a material or preparation to be incorporated into the drug, quasi-drug, or cosmetic.

[0026] The pharmaceutical product (including quasi-drugs, the same applies below) contains eucalyptus or its extract, and peach or its extract as active ingredients for promoting loricrin expression, promoting the formation of the stratum corneum, and improving skin dullness. Furthermore, the pharmaceutical product may contain pharma- ceutical acceptable carriers, other active ingredients, medicinal ingredients, etc., as necessary, so long as the functions of the active ingredients are not lost. The administration form of the pharmaceutical product is arbitrary, but is preferably parenteral administration.The dosage form for parenteral administration includes each preparation for external use on the skin, transdermal, transmucosal, nasal, enteral, injection, suppository, injection, inhalation, patch, etc. Among them, the preferred preparation form is an external use on the skin, specifically, ointment, emulsion, cream, milky lotion, lotion, gel, aerosol, etc.

[0027] The cosmetic contains eucalyptus or an extract thereof, and peach or an extract thereof as active ingredients for promoting loricrin expression, promoting the formation of the stratum corneum, and improving dullness of the skin. Furthermore, the cosmetic may contain a carrier acceptable for cosmetics, or other active ingredients, cosmetic ingredients, etc., as necessary, so long as the functions of the active ingredients are not lost. Preferred examples of cosmetics include face and body cosmetics (for example, lotions, gels, creams, packs, etc.), make-up cosmetics, face or body cleansers, and the like.

[0028] Each of these pharmaceutical and cosmetic preparations can be manufactured according to conventional methods by combining eucalyptus or an extract thereof, and peach or an extract thereof, as necessary, with a pharma- ceutically or cosmetically acceptable carrier, the other active ingredients, medicinal ingredients, cosmetic ingredients, etc., as described above. Examples of the pharma- ceutically or cosmetically acceptable carriers include various oils, surfactants, gelling agents, buffers, preservatives, antioxidants, solvents, dispersants, chelating agents, thickeners, UV absorbers, emulsion stabilizers, pH adjusters, colorants, and fragrances. Examples of such other active ingredients, medicinal ingredients, and cosmetic ingredients include plant extracts, bactericides, moisturizers, anti-inflammatory agents, antibacterial agents, keratolytic agents, cooling agents, antiseborrheic agents, cleansing agents, and makeup ingredients.

[0029] The content of eucalyptus or an extract thereof in the above pharmaceutical or cosmetic formulations cannot be generalized as it varies depending on the form of the formulation. For example, based on the total amount of the formulation, the content is preferably 0.00006% by mass or more, more preferably 0.0001% by mass or more, even more preferably 0.0002% by mass or more, calculated as solid content, and is preferably 0.006% by mass or less, more preferably 0.002% by mass or less, even more preferably 0.0006% by mass or less. Furthermore, the content of peach or its extract in the above-mentioned pharmaceutical or cosmetic formulations cannot be generalized as it varies depending on the form of the formulation, but for example, based on the total amount of the formulation, in terms of solid content, is preferably 0.00003 mass% or more, more preferably 0.00006 mass% or more, even more preferably 0.0003 mass% or more, and is preferably 0.006 mass% or less, more preferably 0.0018 mass% or less, and even more preferably 0.0006 mass% or less.

[0030] In the loricrin expression promoter etc. of the present invention, the ratio of eucalyptus or an extract thereof to peach or an extract thereof, in terms of promoting loricrin expression and promoting stratum corneum formation, is preferably 0.01 or more, more preferably 0.1 or more, even more preferably 0.8 or more by mass [eucalyptus or an extract thereof in terms of solids content / peach or an extract thereof in terms of solids content], and is preferably 300 or less, more preferably 30 or less, and even more preferably 4 or less. The mass ratio [eucalyptus or an extract thereof in terms of solids content / peach or extract thereof in terms of solids content] is preferably 0.01 to 300, more preferably 0.1 to 30, and even more preferably 0.8 to 4.

[0031] The dosage or use amount of Eucalyptus or its extract may be an amount that can achieve the effects of the present invention. The dosage or use amount may vary depending on the species, weight, sex, age, condition, or other factors of the subject, but in the case of parenteral administration such as external skin preparations, the dosage or use amount is preferably 0.0012 μg / cm as the mass of Eucalyptus or its extract in terms of solid content once per adult (60 kg). 2 More preferably, 0.002 μg / cm 2 More preferably, 0.004 μg / cm 2 or more, and preferably 0.12 μg / cm 2 Less than or equal to 0.04 μg / cm 2 Less than 0.012 μg / cm 2 The dosage or use amount of peach or its extract is preferably 0.0006 μg / cm3 per adult (60 kg) once, calculated as the mass of peach or its extract in terms of solid content. 2 More preferably, 0.0012 μg / cm 2 More preferably, 0.006 μg / cm 2 or more, and preferably 0.12 μg / cm 2 Less than or equal to 0.036 μg / cm 2 Less than 0.012 μg / cm 2 The following is the result. In the present invention, such an amount can be administered or used repeatedly or continuously, once or in divided doses per day, for one day or more, preferably seven days or more, more preferably 14 days or more, and even more preferably 42 days or more.

[0032] The subject to which the loricrin expression promoter of the present invention is administered or used is not particularly limited as long as it is a human or a non-human animal that requires or desires it. Preferred examples of subjects include humans who desire promotion of loricrin expression, promotion of stratum corneum formation, and improvement of dull skin. Non-human animals include non-human mammals such as apes and other primates. Furthermore, the site to which the loricrin expression promoter of the present invention is administered or used is not particularly limited, but preferred examples include the face, neck, arms, backs of the hands, fingers, legs, and backs of the feet. EXAMPLES

[0033] Example 1: Loricrin expression promoting effect plant extract The eucalyptus extract used was prepared by extracting eucalyptus leaves with an 80% (v / v) aqueous solution of 1,3-butylene glycol (Maruzen Pharmaceuticals, Eucalyptus Extract BG-KA, solids concentration 0.2% (w / v)). Peach leaf extract was prepared by extracting peach leaves with a 20% (v / v) aqueous solution of 1,3-butylene glycol, and finally preparing a 40% (v / v) aqueous solution of 1,3-butylene glycol (Peach Leaf Liquid B (registered trademark), manufactured by Ichimaru Pharcos Co., Ltd., solids concentration 0.6% (w / v)).

[0034] Experimental cell culture The cells used were normal human epidermal keratinocytes (derived from neonatal foreskin; Kurabo). For cell proliferation and culture, EpiLife Medium, with 60μM calcium (Thermo Fisher) was supplemented with HKGS (Thermo Fisher). For material supplementation culture, EpiLife Medium, with 60μM calcium was supplemented with HKGS Kit (BPE and EGF-free, Thermo Fisher). Cell culture was performed according to standard methods at 37℃ and 5% CO2.

[0035] Protein extraction and loricrin expression analysis Normal human epidermal keratinocytes were cultured in a 6-well plate (collagen-coated; Corning) at 1.5 × 10 5The cells were seeded at a density of 100 cells / well, cultured in a growth medium for one day, then replaced with a medium containing the material and cultured for another day. The next day, eucalyptus extract dissolved in 80% (v / v) 1,3-butylene glycol (added at a concentration of 0.3% (v / v)) and peach leaf extract dissolved in 40% (v / v) 1,3-butylene glycol (added at a concentration of 0.1% (v / v)) were added, and the cells were cultured for 48 hours, washed once with PBS, dissolved in RIPA buffer (SIGMA) containing 100-fold diluted Protease / Phosphatase Inhibitor Cocktail (Cell Signaling), and the cells were collected. The cell suspension was homogenized (ultrasonicated), centrifuged (15,000×g, 10 min), and the supernatant was collected. Protein quantification was then performed using Pierce (registered trademark) BCA Protein Assay Kit (Thermo Fisher). 8 to 10 μg of protein was required for detection. The samples were separated by electrophoresis using NuPAGE (registered trademark) 4-12% SDS (sodium dodecyl sulfate)-polyacrylamide gel (Thermo Fisher), transferred to a PVDF membrane, blocked with TBS-T (Tween 20 0.1% (w / v)) containing 5% (w / v) skim milk for 1 hour, and incubated overnight at 4°C using a primary antibody (Anti-Loricrin antibody; Abcam) diluted 2000 times in blocking solution. After washing three times for 15 minutes with PBS-T, the samples were incubated at room temperature for 1 hour using a secondary antibody (anti-rabbit IgG antibody (HRP labeling); Dako) diluted 4000 times in blocking solution. After washing three times for 10 minutes with PBS-T, images were taken with a detector (Amersham Imager 600) using the luminescence reagent SuperSignal (registered trademark) West Dura Extended Duration substrate (Thermo Fisher). Band concentrations were quantified using an Amersham Imager 600. The measurement data was evaluated relative to the amount of loricrin protein expression, with the value of the control group set at 1. The results are shown in Table 1.

[0036] [Table 1]

[0037] As is clear from Table 1, it was confirmed that in the group to which eucalyptus extract and peach leaf extract were added in combination, the expression of loricrin was synergistically promoted more than in the groups to which eucalyptus extract or peach leaf extract was added alone.

Claims

1. A loricrin expression promoter comprising eucalyptus or an extract thereof, and peach or an extract thereof as active ingredients.

2. A stratum corneum formation promoter containing eucalyptus or an extract thereof, and peach or an extract thereof as active ingredients.

3. A skin dullness improving agent containing eucalyptus or its extract, and peach or its extract as active ingredients.

4. The agent according to any one of claims 1 to 3, wherein the mass ratio of eucalyptus or an extract thereof to peach or an extract thereof (solid content of eucalyptus or an extract thereof / solid content of peach or an extract thereof) is 0.01 or more and 300 or less.

5. The agent according to any one of claims 1 to 3, which is in the form of an external preparation for skin.