Xenopus self-sufficient culture system

Abstract

To provide a self-sufficient culture system for culturing the feed of Xenopus.SOLUTION: There is provided a Xenopus self-sufficient culture system, including: a groundwater storage tank for storing groundwater; an ozone bubble water tank for generating ozone bubbles from the groundwater; nano ozone bubble water generated through a nano nozzle in the ozone bubble water tank; a Xenopus breeding water tank for breeding Xenopus with the nano ozone bubble water; a first filtration tank for filtering and purifying Xenopus breeding water; an aquatic Tubifex tubifex breeding tank for breading aquatic Tubifex tubifex as a feed for the Xenopus with filtered-drained nano ozone produced by passing the filtered wastewater through a nano nozzle; a terrestrial earthworm breading tank for breading terrestrial earthworms with aquatic Tubifex tubifex breeding water; and a second filtration tank for resending the drained water from the aquatic Tubifex tubifex breading tank to the ozone bubble tank for circulation of the drained water.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a self-sufficient farming system for Xenopus frogs, and in particular to a self-sufficient farming system for Xenopus frogs in which, in order to obtain a food processing raw material that is high in protein and rich in amino acids from Xenopus frogs, Xenopus frogs are farmed, the breeding water is filtered, and the filtered wastewater is used to farm aquatic tubifex worms and terrestrial earthworms to be fed to the Xenopus frogs, and these are sterilized before being fed to the Xenopus frogs. [Background technology]

[0002] Edible frogs, which are used as a food source, are rich in nutrients, and amino acid analysis has proven that they are particularly rich in amino acids necessary for the growth of living organisms.

[0003] It is important that a good amino acid contains a balanced amount and type of amino acids that the human body needs. It is desirable that it contains a lot of essential amino acids, which cannot be synthesized in the body. It is also desirable that it contains lysine, threonine, methionine, and other amino acids known to be components of high-quality proteins. Another characteristic of good amino acids is that they exist in a form that is easy to absorb and digest.

[0004] The amino acids most necessary for humans are the "essential amino acids" that cannot be synthesized in the body. There are nine essential amino acids: arginine, leucine, isoleucine, valine, lysine, methionine, phenylalanine, threonine, tryptophan, and histidine.

[0005] FIG. 7 is an amino acid analysis table of Xenopus laevis.

[0006] Figure 8 shows the values, with the essential amino acid Trp (tryptophan) set at 1. The total of these values, 56.0, indicates that Xenopus laevis has a good balance of essential amino acids, containing all the amino acids required by humans in good balance; this value is high, and it is expected to be an excellent source of protein.

[0007] Figure 9 shows the nutritional value of 100g of edible frog. Frogs contain more protein than fish and also contain more vitamins.

[0008] By the way, species of edible frogs include the European tree frog and bullfrog in Europe and the United States, the tiger frog, marsh frog, and Indian grass frog in Asia (China, Indonesia, and Vietnam), the Nanbei bullfrog in South America, the African clawed frog in Africa, and the Namiye frog and hairy frog (presumably the brown frog) in Japan.

[0009] In the past, Japan farmed bullfrogs and exported them to the United States as canned products, which was a cash cow, but export was banned after pesticides were detected in the products.

[0010] As a result, the demand for bullfrogs disappeared, and they were released into the wild, where they began to inhabit all over the country and have a negative impact on native species.As environmental conservation is required, they have been designated as a specified alien species, and their movement and cultivation are prohibited.

[0011] It is not a popular food in Japan, where food is abundant, protein is obtained from fish, poultry and other animal meat, and people have a fondness for frogs.

[0012] In addition, it is prohibited by law to eat wild frogs. On the other hand, in Japan, they are sometimes kept as pets, and there is demand for them among enthusiasts and collectors, but the demand for them as food is not so high.

[0013] On the other hand, there are regions around the world where edible frogs are actually eaten, mainly in Europe, China, Vietnam, Cambodia, Thailand, Taiwan, Singapore, Indonesia, Malaysia, South Africa, the United States, and many other countries where they are used as a food ingredient.

[0014] The preparation methods include frog fillets and legs, fried foods, grilled foods, steaks, stews, and other meat dishes. Leg meat is cooked with glutinous rice and used as an ingredient in soups.

[0015] Stock or broth can be made from the bones, innards, and skin of the frog, and vegetables and spices can be added to the soup to adjust the flavor.

[0016] Additionally, frogs can be canned to preserve them for a longer period of time. When canning, the meat and broth are packed into a can and then sterilized by heat.

[0017] Frog legs can also be used to make fried snacks, and spices can be added to increase the variety of flavors. As you can see, there are many ways to prepare edible frogs.

[0018] Examples of food made from African clawed frogs include meat, which has a flavor similar to chicken or fish. Processed products made from frog meat include hamburgers, sausages, meatballs, and pates.

[0019] Fried chicken and fried dishes are common in Africa and other places. By being fried, they become easy to eat and familiar foods.

[0020] The frog meat and vegetable filling wrapped in dumpling skin is healthy and nutritious, making it popular among health-conscious people.

[0021] African frog curry is a dish made by simmering meat in a tomato-based curry sauce. It is spicy, tasty, and rich in protein, making it popular among health-conscious people.

[0022] However, in Japan, some people use imported edible frogs as food. Although they are an excellent food, they are not very popular.

[0023] By the way, the main countries with large production volumes of farmed edible frogs are China, France, Vietnam, Indonesia, the United States, etc. China is the largest producer, accounting for about 90% of the world's production. France is also one of the important producing countries, and because of the demand as a luxury food, the production of canned and processed products is also large. There are no exact figures for the market size, but there is a certain market in regions and countries where demand is high.

[0024] When cultivating these edible frogs in developed countries, attention must be paid to the productive, environmental and safety issues of aquaculture. In particular, attention must be paid to the sounds of the frogs and wastewater treatment. When mass-producing edible frogs, their croaking during the breeding season becomes loud, causing noise to nearby residences and adversely affecting the living environment of the residents.

[0025] The best frogs to farm are African clawed frogs. The main reasons for this are that African clawed frogs do not make noise, so there is no noise for the surrounding area, they live in water and do not live on land, and furthermore, they can be artificially inseminated, making them ideal for production-controlled farming.

[0026] However, farming Xenopus frogs (including other edible frogs) entails several challenges, such as the cultivation of feed and water quality management. Problems with the production system include safety, hygiene, and the treatment of feces and wastewater from edible frogs when used as food.

[0027] In addition, proper management is required for its production and distribution from the standpoints of sustainability, animal welfare, hygiene, etc.

[0028] For this reason, the present applicant has proposed a "system for circulating rearing water for cultivating edible frogs" and a "system for circulating rearing water for cultivating feed for edible frogs" in Patent Documents 1 and 2.

[0029] Patent Document 1 proposes a breeding water circulation system for cultivating edible frogs, comprising an electrolytic cell that generates slightly acidic electrolyzed water, a slightly acidic electrolytic water tank that stores the slightly acidic electrolyzed water generated in the electrolytic cell, a diluted electrolyzed water tank that stores nano hypochlorous acid water obtained by adding nanobubbles and dilution water to the slightly acidic electrolyzed water supplied from the slightly acidic electrolytic water tank, an edible frog breeding tank that uses the nano hypochlorous acid water generated in the diluted electrolyzed water tank as breeding water for edible frogs, a purification tank that filters and purifies the breeding water used in the edible frog breeding tank, and a purified water tank that sterilizes and stores the filtered and purified breeding water, and the sterilized breeding water is supplied to the electrolytic cell and circulated within the facility.

[0030] Patent Document 2 proposes a breeding water circulation system for cultivating edible frog food, the system comprising: an underground freshwater storage tank for storing underground freshwater; an ozone generator for generating ozone in the underground freshwater stored in the underground freshwater storage tank to sterilize it; a nanobubble generator for generating nanobubbles in the underground freshwater stored in the underground freshwater storage tank to increase the dissolved oxygen concentration; edible frog food consisting of larvae or juveniles of amphibians or freshwater fish; a breeding tank for edible frog food in which the ozone nanobubble water generated in the underground freshwater storage tank is used as breeding water for the edible frog food; a purification tank for filtering and purifying the breeding water used in the breeding tank for edible frog food; and a purified water tank for storing the filtered and purified breeding water, the system being characterized in that the purified breeding water is sent to the underground freshwater storage tank and the ozone generator and the nanobubble generator are operated to generate ozone nanobubble water and circulate it within the facility.

[0031] According to Patent Documents 1 and 2, the breeding water (13°C to 28°C) has a high dissolved oxygen concentration and eliminates bacteria, which increases the hatching rate and survival rate of larvae, shortens the growth process of edible frogs and seafood, and eliminates the hibernation period of edible frogs.

[0032] In addition, this nano-hypochlorous acid water can be discharged outside as safe breeding water by further enhancing its disinfecting effect through electrolysis at 30℃ to 70℃ in a single-chamber electrolytic cell.

[0033] In addition, nano-ozone bubble water from groundwater is effective in increasing hatching rates, survival rates of young fish and larvae (tadpoles), and shortening the growth period. Nano-hypochlorous acid water is also effective in decreasing hatching rates, survival rates of young fish and larvae (tadpoles), and shortening the growth period. Therefore, nano-ozone bubble water and nano-hypochlorous acid water can speed up the growth period of fish and edible frogs, and their sterilization effect allows them to be raised with a high survival rate. By completely cultivating the food of edible frogs raised in this breeding water, the cost of cultivating edible frogs, which can be used as alternative fish meal, can be significantly reduced. [Prior art documents]

[0034] [Patent Document 1] Patent No. 6799887 [Patent Document 2] Patent No. 7079302 Summary of the Invention [Problem to be solved by the invention]

[0035] However, there are problems associated with culturing Xenopus frogs, such as the following: That is, when cultivating edible frogs, particularly African clawed frogs, caution is required regarding chytridiomycosis and ranavirus disease as a safety issue. Chytridiomycosis is asymptomatic and is transmitted to humans through African clawed frogs. It is a frog disease originating in Africa and was introduced to Japan through imported frogs. It is an emerging infectious disease in amphibians and belongs to the phylum Chytridiomycosis.

[0036] Chytrid fungus is a fungus that lives in soil and freshwater and parasitizes the skin of amphibians. It can be fatal if infected, but it can be treated at specialized institutions. It does not infect humans, fish, mammals, birds, or reptiles. Purpose of the invention

[0037] Therefore, the object of the present invention is to provide a self-sufficient farming system that is highly safe, can increase the supply, and can reduce costs when farming Xenopus frogs to obtain food raw materials that are high in protein and rich in amino acids, and that uses filtered wastewater from the farming water to farm Xenopus frogs as food.

[0038] In order to achieve the above object, the present invention provides a groundwater storage tank for storing groundwater, an ozone bubble tank for generating ozone bubbles from the groundwater stored in the groundwater storage tank, nano-ozone bubble water generated through a nano-nozzle in the ozone bubble tank, an Xenopus frog breeding tank into which the nano-ozone bubble water is injected and in which Xenopus frogs are raised in the nano-ozone bubble water, a first filtration tank for filtering and purifying the Xenopus frog breeding water used in the Xenopus frog breeding tank, and a filtered wastewater filtered in the first filtration tank through a nano-nozzle. The present invention provides a self-sufficient aquaculture system for Xenopus frogs, the system comprising: an aquatic tubifex breeding tank into which the filtered wastewater nano-ozone water is generated, the filtered wastewater nano-ozone water is injected and used to breed aquatic tubifex worms that are food for the Xenopus frogs; a terrestrial earthworm breeding tank in which terrestrial earthworms that are food for the Xenopus frogs are breeded in water for breeding aquatic tubifex worms using the filtered wastewater nano-ozone water; and a second filtration tank in which discharged water discharged from the aquatic tubifex breeding tank is re-supplied to the ozone bubble tank for circulating the water.

[0039] In the above configuration, the self-sufficient farming system for Xenopus frogs further includes a single-chamber electrolytic cell in which the anode and cathode are not separated by a diaphragm, and the aquatic tubifex and terrestrial earthworms that are to be fed to the Xenopus frogs are sterilized with weak hypochlorous acid water produced in the single-chamber electrolytic cell, and are then fed to the Xenopus frogs, thereby promoting a sterilization action for sterilization before shipping.

[0040] The aquatic tubifex worm breeding tank has an aquatic tubifex worm bed which serves as a bed for the aquatic tubifex worms, and the aquatic tubifex worm bed is characterized in that it has a resin net-like shape.

[0041] The terrestrial earthworm cultivation tank is characterized by having culture soil for cultivating the terrestrial earthworms, nano nozzles for spraying water on the culture soil to maintain an appropriate humidity level, and a compressor and air tubes for injecting an appropriate amount of oxygen into the culture soil.

[0042] The self-sufficient aquaculture system for Xenopus frogs according to claim 1, characterized in that the terrestrial earthworm rearing tank is provided with LED lighting at the top of the tank.

[0043] The self-sufficient aquaculture system for Xenopus frogs is further characterized by having a sterilization tank for aquatic / terrestrial earthworm feed in which the aquatic tubifex worms and terrestrial earthworms are sterilized for use as feed, and an Xenopus shipping sterilization tank in which the cultivated Xenopus frogs are sterilized before shipping.

[0044] The self-sufficient culturing system for Xenopus frogs further comprises a wastewater heat treatment external discharge tank for heating the rearing water and sterilized water discharged from the terrestrial earthworm rearing tank, the aquatic / terrestrial earthworm food sterilization tank, and the Xenopus frog shipping sterilization tank to 70°C or higher and discharging the water to the outside.

[0045] The nano-ozone bubble water used in the self-sufficient aquaculture system for Xenopus frogs of the present invention is breeding water (10℃ to 30℃) with a high dissolved oxygen concentration that sterilizes bacteria, and has the effect of increasing the hatching rate and survival rate of larvae and promoting the growth of Xenopus frogs.

[0046] Nano-ozone bubble water is effective in shortening the hatching rate, survival rate and growth period of African clawed frogs (juveniles and tadpoles). Therefore, nano-ozone bubble water can speed up the growth period of African clawed frogs and increase the survival rate due to its sterilization effect. By cultivating aquatic worms and terrestrial earthworms bred in this breeding wastewater, it is possible to significantly reduce the cost of cultivating edible frogs, which are high in protein and rich in amino acids and are used as raw materials for food processing.

[0047] Furthermore, the aquatic worms and terrestrial earthworms that are used as food are rich in protein and amino acids, which further enhance the protein, amino acids, vitamins, and other components of farmed Xenopus frogs, making them an excellent food ingredient.

[0048] By feeding them, it is possible to obtain farmed Xenopus frogs that are superior to conventional Xenopus frogs. [Brief description of the drawings]

[0049] [Figure 1] FIG. 1 is a schematic diagram of a self-sufficient Xenopus laevis farming system. [Diagram 2] Schematic diagram of an underground water tank, an ozone bubble tank, and nano nozzle bubbles. [Diagram 3] FIG. 1 is a diagram showing the treatment of residual food and wastewater generated in an aquarium for rearing Xenopus laevis. [Figure 4] Diagram showing the bedding of an aquatic tubifex worm. (a) is a plan view, and (b) is a cross-sectional view. [Diagram 5] FIG. 2 is a cross-sectional view of a terrestrial earthworm rearing tank. [Figure 6] FIG. 1 shows the sterilization of rearing water using weak hypochlorous acid water produced by electrolyzing groundwater, and the flow of the water discharged after sterilization. [Figure 7] This is an amino acid analysis table for Xenopus laevis. [Figure 8] This number is calculated with the essential amino acid Trp (tryptophan) being 1. [Figure 9]This is a nutritional table for 100g of the edible portion of edible frog. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0050] Hereinafter, a culture water circulation system for cultivating Xenopus frogs according to an embodiment of the present invention will be described in detail with reference to the drawings.

[0051] Hereinafter, a self-sufficient culturing system for cultivating Xenopus frogs according to an embodiment of the present invention will be described in detail with reference to the drawings.

[0052] FIG. 1 is a schematic diagram of a self-sufficient aquaculture system for Xenopus laevis. Here, ▲1▼ is an underground water storage tank, and the water temperature is 10℃ or higher, which is a temperature at which Xenopus frogs do not hibernate. ▲2▼ is a single-chamber electrolytic cell in which the anode and cathode are not separated by a diaphragm, and produces slightly acidic electrolyzed water (hypochlorous acid water = HClO) that promotes sterilization for use in sterilizing Xenopus frogs when they are fed or shipped.

[0053] As shown in Figure 2, groundwater stored in the groundwater storage tank ▲1▼ is supplied to the ozone bubble tank ▲3▼ to generate ozone bubbles, which are then passed through the nano nozzle ▲4▼ to generate nano-ozone bubble water, promoting the sterilization of the groundwater. At the same time, the nano-ozone bubble water increases the dissolved oxygen concentration, which penetrates into the skin of Xenopus frogs, making them slippery and promoting their growth.

[0054] As shown in Figure 3, residual food and wastewater generated in the Xenopus breeding tank ▲5▼ is filtered through a filter ▲7▼ installed in the filtration tank ▲6▼.

[0055] The filtered water is then used to generate nano-ozone water using a nano-bubble nozzle ▲9▼, which is then injected into the aquatic tubifex worm breeding tank ▲10▼, where the tubifex worms are raised in an environment with a high dissolved oxygen concentration. As shown in Figures 4(a) and (b), the aquatic tubifex worm bedding ▲11▼ is in the form of a resin net, and is a bedding that does not allow the worms to attach, making it easier to collect the tubifex worms that are used as food. The remaining food and wastewater generated in the aquatic tubifex worm breeding tank ▲10▼ are filtered again through a filter (not shown) installed in the filtration tank ▲6▼A, and the filtered water is sent to the ozone bubble tank ▲3▼.

[0056] Meanwhile, as shown in Figure 5, in the terrestrial earthworm cultivation tank ▲13▼, culture soil ▲14▼ is provided, and water is sprayed with a watering nozzle ▲12▼ to maintain an appropriate humidity. The water used here is nano-ozone water generated by a nano nozzle ▲9▼. In order for terrestrial earthworms to live, it is necessary to maintain an appropriate amount of oxygen in the culture soil ▲14▼. For this purpose, a compressor ▲17▼ is provided to inject oxygen into the culture soil ▲14▼ through an air tube ▲15▼, and an LED light ▲16▼ is installed above the terrestrial earthworm cultivation tank ▲13▼ to prevent escape at night.

[0057] Next, referring to Figs. 1 and 6, the sterilization tank for aquatic and terrestrial earthworm feed and the sterilization tank for shipping Xenopus laevis will be described. Reference numeral 18 denotes a sterilization tank for aquatic and terrestrial earthworm bait in which sterilization treatment is performed for aquatic and terrestrial earthworm bait, and reference numeral 19 denotes a Xenopus shipping sterilization tank in which Xenopus shipping sterilization treatment is performed. Groundwater from the groundwater storage tank ▲1▼ is pumped into a single-chamber electrolytic tank ▲2▼ to generate weak hypochlorous acid water, which is then pumped into a sterilization tank ▲18▼ for aquatic and terrestrial earthworm food to sterilize the aquatic worms and terrestrial earthworms that are to be fed to Xenopus frogs. When shipping Xenopus frogs, they are sterilized in the Xenopus shipping sterilization tank ▲19▼ and shipped as a safe and secure food ingredient.

[0058] When the breeding water and sterilized water are discharged to the outside, they are heated to 70°C or higher in the wastewater heat treatment external discharge tank 20 and then discharged to the outside 21.

[0059] In order for frogs not to hibernate, it is necessary to keep the temperature above 10°C. In particular, frogs born in tropical regions do not hibernate. If you try to reverse this, they may die. If you control the water temperature, they will not hibernate. In particular, African clawed frogs live in water and not on land, so there is no need to control humidity.

[0060] By the way, there are applications for food processing products that are specific to farmed African clawed frogs.

[0061] For example, processed ingredients are used in food processing with food additives whose main ingredients are farmed African clawed frog (including other edible frog) powder, oil, egg ovum, and solubles.

[0062] Powder made from farmed African clawed frogs (including other edible frogs) is made by boiling them, separating the fat and water in a press, and then drying them to reduce the moisture content to less than 10%.

[0063] And farmed African clawed frog oil (including other edible frogs) is made by refining the oil obtained by boiling the frogs and separating the fat and water in a press.

[0064] Furthermore, egg cells of cultured Xenopus laevis (including other edible frogs) are obtained by centrifuging the egg cells, and the egg yolk, egg extract and / or lipids are obtained.

[0065] Soluble matter from farmed African clawed frogs (including other edible frogs) is obtained by centrifuging the cooking liquid and compressed liquid that are by-products of the powder manufacturing process, and then removing solids and fats and oils, resulting in a concentrated solution.

[0066] The powder of cultured Xenopus laevis (including other edible frogs) may be combined and mixed with any of the following: oil of cultured Xenopus laevis (including other edible frogs), egg cells of cultured Xenopus laevis (including other edible frogs), solubles of cultured Xenopus laevis (including other edible frogs), or any combination of these, or with all food additives.

[0067] Cultured African clawed frog (including other edible frogs) oil is mixed with any or all of the following food additives: egg cells of cultured African clawed frog (including other edible frogs) and solubles of cultured African clawed frog (including other edible frogs).

[0068] The egg cells of the eggs of the farmed African clawed frog (including other edible frogs) are mixed with foodstuff blending raw materials, including solubles from the farmed African clawed frog (including other edible frogs) and foodstuff additives.

[0069] Farmed Xenopus laevis (including other edible frogs) powder, farmed Xenopus laevis (including other edible frogs) oil, farmed Xenopus laevis (including other edible frogs) egg cells and farmed Xenopus laevis (including other edible frogs) solubles are produced from farmed Xenopus laevis (including other edible frogs).

[0070] Powder of cultured Xenopus frogs (including other edible frogs), oil of cultured Xenopus frogs (including other edible frogs) and / or egg cells of cultured Xenopus frogs (including other edible frogs) and / or solubles of cultured Xenopus frogs (including other edible frogs) can provide a food ingredient that is high in protein, rich in amino acids and well-balanced when used with food additives.

[0071] Farmed African clawed frogs (including other edible frogs) are high in protein, low in fat and low in calories, making them highly nutritious and allowing them to be cooked in a variety of ways. As a result, demand for them is increasing along with growing health consciousness and diversifying food culture.

[0072] Farmed African clawed frogs (and other edible frogs) are highly nutritious, especially rich in essential amino acids, allowing the development of a variety of processed products.

[0073] One example is the development of high-protein processed foods made from farmed African clawed frogs (and other edible frogs). In modern society, where protein is often insufficient, there is a demand for high-protein foods.

[0074] We can also provide health foods that use nutrients extracted from the meat and organs of farmed African clawed frogs (and other edible frogs).

[0075] For example, supplements containing amino acids and peptides, and beauty and health foods containing collagen and hyaluronic acid.

[0076] Furthermore, it is possible to develop seasonings and soups using stock and extracts from farmed African clawed frogs (and other edible frogs). These have a strong flavor and can be used as a base for soups and stocks to add flavor and umami.

[0077] As described above, the development of processed products from farmed Xenopus frogs (and other edible frogs) has potential in a variety of fields.

[0078] Powdered raw materials from farmed African clawed frogs (and other edible frogs) can also be used in health-related products such as health foods and nutritional supplements. These food products are rich in protein, which is necessary for muscle growth and repair, and is an important nutrient for athletes, trainees, dieters, and others.

[0079] The following describes a method for processing raw materials that are rich in protein, and is a processing and manufacturing method that is specialized for farmed Xenopus laevis.

[0080] In the hydrolysate processing method, farmed African clawed frogs are heated and pressurized at high temperatures and pressures to break down the proteins and extract amino acids and peptides. The extracted nutrients are easily digested and absorbed by the body, making them suitable for use as sports nutrition and diet foods.

[0081] The extraction method involves extracting nutrients from the internal organs and bones of farmed African clawed frogs, and collagen extracted from farmed African clawed frog meat is used in cosmetics and health foods.

[0082] The drying method involves drying the meat and internal organs of farmed African clawed frogs to extract nutrients. The extracted nutrients can be stored for long periods of time and can be used in processed foods and pet food.

[0083] The hydrogenation method involves hydrogenating the meat and internal organs of farmed Xenopus frogs to produce hydrogenated proteins and hydrogenated collagen. The produced nutrients are easy to digest and absorb, have good taste, and are highly stable, making them suitable for use as health and beauty foods.

[0084] Using the above methods, nutrients are extracted from meat and organs to produce various processed products. Some products may contain chemical seasonings and preservatives, but additive-free products are becoming more common these days.

[0085] Powder, oil, egg cells, and solubles from farmed Xenopus frogs (including other edible frogs) produced in a self-sufficient farming system that reduces production costs and emphasizes hygiene and food safety are food processed products that are blended and mixed with food additives.

[0086] In food additives used in powder, solubles, oils, and egg cells of farmed African clawed frogs (including other edible frogs), Preservatives are sorbic acid, potassium sorbate, butylparaben, ethylparaben, methylparaben, propylparaben, and sodium benzoate. Acidulants are citric acid, malic acid, and acetic acid. Sweeteners are sugar, glucose, honey, sucralose, aspartame, acesulfame K, saccharin, and sucralose. The pigments are carotenoids, anthocyanins, gardenia pigments, caramel pigments, and sodium nitrite. Flavoring agents: vanillin, cinnamon aldehyde, methylcyclopentadienone. Thickeners are xanthan gum, gum arabic, and thickening polysaccharides. Color formers are nitrates, nitrites, and zinc salts. Leavening agents are sodium bicarbonate and citrate. The stabilizer is phosphate. The emulsifiers are sorbitan fatty acid ester, polyglycerol fatty acid ester, and lecithin. The seasonings are glutamic acid, inosinic acid, and guanylic acid. The above are mixed with food additives.

[0087] The safety of the above food additives has been confirmed depending on the type and amount used in each food.

[0088] The raw materials are powder, solubles, oil, and egg cells of farmed African clawed frogs (including other edible frogs), in other words, African clawed frogs (including other edible frogs) farmed in a self-sufficient, recirculating farming system that can ensure cost, environmental friendliness, hygiene, safety, quality, etc.

[0089] However, farming Xenopus frogs (and other edible frogs) presents several challenges, including feed cultivation and water quality management.

[0090] Therefore, the production and distribution of farmed Xenopus frogs (including other edible frogs) requires appropriate management from the standpoints of sustainability, animal welfare, hygiene, etc.

Claims

1. a groundwater storage tank for storing groundwater; An ozone bubble tank for generating ozone bubbles from the groundwater stored in the groundwater storage tank; Nano ozone bubble water generated through a nano nozzle in the ozone bubble water tank; a tank for breeding Xenopus frogs into which the nano-ozone bubble water is injected and in which Xenopus frogs are raised in the nano-ozone bubble water; a first filtration tank for filtering and purifying the Xenopus laevis breeding water used in the Xenopus laevis breeding tank; A filtered wastewater nano-ozone water is generated by passing the filtered wastewater through a nano nozzle, an aquatic tubifex breeding tank into which the filtered wastewater nano-ozone water is poured and in which the aquatic tubifex worms that serve as food for the Xenopus laevis are raised with the filtered wastewater nano-ozone water; A terrestrial earthworm breeding tank in which terrestrial earthworms to be fed to the African clawed frog are raised in the breeding water of aquatic worms using the filtered wastewater nano-ozone water; A second filtration tank for recirculating the discharged water discharged from the aquatic worm breeding tank to the ozone bubble tank; A self-sufficient farming system for Xenopus frogs.

2. The self-sufficient aquaculture system for Xenopus frogs described in claim 1 further comprises a single-chamber electrolytic cell in which the anode and cathode are not separated by a diaphragm, and the aquatic tubifex worms and terrestrial earthworms that are to be fed to the Xenopus frogs are sterilized with weak hypochlorous acid water produced in the single-chamber electrolytic cell, and then fed to the Xenopus frogs, thereby promoting a sterilization action for sterilization before shipping.

3. 3. A self-sufficient aquaculture system for Xenopus frogs as described in claim 1 or 2, characterized in that the aquatic worm breeding tank has an aquatic worm bed that serves as a sleeping place for the aquatic worms, and the aquatic worm bed has a resin net shape.

4. The self-sufficient aquaculture system for Xenopus frogs described in claim 1, characterized in that the terrestrial earthworm cultivation tank has culture soil for cultivating the terrestrial earthworms, and has a nano nozzle for spraying water to maintain an appropriate humidity level on the culture soil, and a compressor and air tube for injecting an appropriate amount of oxygen into the culture soil.

5. 2. A self-sufficient aquaculture system for Xenopus frogs according to claim 1, characterized in that the terrestrial earthworm rearing tank is provided with LED lighting at the top of the tank.

6. The self-sufficient aquaculture system for Xenopus frogs as described in any one of claims 1 to 5, further comprising an aquatic / terrestrial earthworm feed sterilization tank in which the aquatic tubifex worms and terrestrial earthworms are sterilized for use as feed, and an Xenopus shipping sterilization tank in which the cultivated Xenopus frogs are sterilized at the time of shipment.

7. The self-sufficient aquaculture system for Xenopus frogs described in any one of claims 1 to 6, further comprising a wastewater heat treatment external discharge tank in which the breeding water and sterilized water discharged from the terrestrial earthworm rearing tank, the aquatic and terrestrial earthworm feed sterilization treatment tank, and the Xenopus frog shipping sterilization treatment tank are heated to 70°C or higher and discharged to the outside.

Images