Generation method for cosmetic composition

Abstract

To provide a cosmetic apparatus that exerts an effect excellent in improvement of a skin state and aging prevention, and a beauty care method.SOLUTION: A cosmetic apparatus 1 comprises a mixer 2 for mixing a stem cell culture supernatant liquid and elastin together, and an introducer 3 for introducing a mixed liquid, in which the stem cell culture supernatant liquid and the elastin are mixed together by the mixer 2, into the skin. The cosmetic apparatus 1 can mix the stem cell culture supernatant liquid and the elastin together by the mixer 2 and introduce the mixed liquid into the skin by the introducer 3. Consequently, the cosmetic apparatus 1 can achieve improvement of a skin state and aging prevention.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to beauty The present invention relates to a method for producing a composition for use. [Background technology]

[0002] Various devices have been proposed for improving skin condition and preventing aging by introducing a cosmetic composition into the skin. For example, Patent Document 1 discloses a cosmetic treatment device for introducing human stem cells, hyaluronic acid, etc. from the epidermis to the dermis. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Utility Model Registration No. 3239242 Summary of the Invention [Problem to be solved by the invention]

[0004] The applicant has discovered a cosmetic device and method that is more effective in improving skin condition and preventing aging than when a cosmetic composition is applied to the skin alone.

[0005] The object of the present invention is to provide a composition that is effective in improving skin condition and preventing aging. Rumi The present invention provides a method for producing a composition for use. [Means for solving the problem]

[0006] The cosmetic device according to the first aspect of the present invention comprises a mixer that mixes stem cell culture supernatant and elastin, and an introducer that is connected to the mixer via a tube and that introduces the mixed liquid into the skin when the mixed liquid, produced by mixing the stem cell culture supernatant and elastin with the mixer, flows in via the tube, and is characterized in that the introducer has a head having a plurality of micro-nozzles from which the mixed liquid is sprayed.

[0007] According to the first aspect, the cosmetic device can mix the stem cell culture supernatant and elastin using a mixer and introduce the mixture into the skin using an introducer, thereby improving the condition of the skin and preventing aging.

[0008] In the first embodiment, the stem cell culture supernatant may be a human stem cell culture supernatant. The stem cell culture supernatant may be an umbilical cord-derived or amniotic membrane-derived stem cell culture supernatant. The introducer may introduce the mixture into the skin within 15 to 30 minutes after the mixer mixes the stem cell culture supernatant and elastin. The elastin may contain desmosine and isodesmosine. In these cases, the cosmetic device can improve the effect of improving the condition of the skin and preventing aging.

[0009] In the first embodiment, the head may be capable of outputting electromagnetic waves of 30 MHz to 300 MHz. In these cases, the cosmetic device can increase the efficiency of introducing the mixed liquid into the skin, thereby improving the effects of improving the condition of the skin and preventing aging.

[0010] The method for producing a cosmetic composition according to the second aspect of the present invention includes a mixing step of mixing stem cell culture supernatant and elastin, and produces a cosmetic composition in which the stem cell culture supernatant and elastin are mixed. According to the second aspect, it is possible to achieve the same effects as the first aspect.

[0011] In the second embodiment, the stem cell culture supernatant may be a human stem cell culture supernatant. The stem cell culture supernatant may be an umbilical cord-derived or amniotic membrane-derived stem cell culture supernatant. The elastin may contain desmosine and isodesmosine. In these cases, the same effects as those of the first embodiment can be achieved. [Brief description of the drawings]

[0012] [Figure 1] FIG. 1 is a diagram showing a cosmetic device 1. [Diagram 2] 1 is a flowchart showing a cosmetic method. [Diagram 3]Photographs showing the condition of the skin before and after treatment. [Figure 4] Photographs showing the condition of the skin before and after treatment. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0013] An embodiment of a beauty device 1 according to the present invention will be described with reference to the drawings. The drawings are used to explain technical features that may be adopted by the present invention. The configurations of the device described are merely illustrative examples and are not intended to limit the scope of the present invention.

[0014] In recent years, regenerative medicine and cell therapy using stem cells have been attracting attention. Stem cells are special cells that have the ability to self-replicate and the ability to differentiate into various cells (differentiation ability). Because they have these two abilities, stem cells are thought to be the cells responsible for tissue development and regeneration, and are attracting attention.

[0015] Stem cell culture supernatant, which is a culture medium for stem cells, is the supernatant generated when culturing stem cells. Stem cell culture supernatant is known to contain many growth factors and cytokines with excellent physiological activity. These growth factors and cytokines are said to have the effect of suppressing and preventing aging and damage of the human skin. For this reason, research is being conducted on the application of stem cell culture supernatant to cosmetic compositions.

[0016] Human skin has a basal layer between the epidermis and dermis. The basal layer is located at a depth of about 0.2 mm from the skin surface. In the basal layer, new cells are produced by repeated cell division and supplied to the epidermis and dermis. For this reason, activating the basal layer is important for improving skin condition and preventing aging. The present invention uses stem cell culture supernatant as one of the cosmetic compositions for activating the basal layer.

[0017] As the stem cells used in the process of producing the stem cell culture supernatant, mesenchymal stem cells are preferably used. The stem cells are not limited to those derived from a specific tissue, and may be derived from any tissue. For example, they may be derived from bone marrow, umbilical cord, umbilical cord blood, placenta, dental pulp, adipose tissue, amniotic membrane, etc. Among these, umbilical cord or amniotic membrane stem cells are particularly preferably used. In addition, as the donor of the stem cells, from the viewpoints of avoiding the risk of allergy and affinity, it is preferable that the donor is a human, but it may also be a pig, cow, sheep, horse, etc. In addition, the donor is not limited to an animal, and may also be a plant.

[0018] In addition, in the present invention, a mixture of stem cell culture supernatant and elastin is used as a cosmetic composition. Elastin is known as a major cosmetic ingredient along with collagen and hyaluronic acid. Elastin binds collagen fibers together in the dermis. Elastin is known to contribute to the firmness and elasticity of the skin and to prevent wrinkles and sagging.

[0019] As the elastin to be mixed with the stem cell culture supernatant, elastin containing cross-linked amino acids (desmosine and isodesmosine) is preferably used. Desmosine and isodesmosine are special amino acids contained only in elastin, and have the function of cross-linking peptide chains. The content of desmosine and isodesmosine is not particularly limited, but for example, high-purity elastin containing 1.0% or more of desmosine and isodesmosine is preferably used. High-purity elastin has an amino acid composition similar to that of human elastin and is water-soluble, so it is suitable for absorption into the skin. In addition, high-purity elastin has the advantage of being heat-resistant and not denaturing beauty ingredients.

[0020] As the elastin, it is possible to use elastin derived from pigs or fish, with pig-derived elastin being particularly preferred.

[0021] Furthermore, the mixture is introduced into the subject's skin within 15 to 30 minutes after the stem cell culture supernatant and elastin are mixed. In other words, the mixture is generated immediately before being introduced into the subject's skin. In this case, the effect of improving the skin condition and preventing aging can be enhanced compared to the case where the mixture is stored for a long time after the stem cell culture supernatant and elastin are mixed and then introduced into the skin.

[0022] <Overview of Beauty Device 1> 1, the cosmetic device 1 includes a mixer 2, an introducer 3, and a control device 4. The cosmetic device 1 is used to mix stem cell culture supernatant and elastin and introduce the mixture into the skin of a subject.

[0023] Mixer 2 has a syringe 21 and a piston 22. Stem cell culture supernatant and elastin are enclosed in syringe 21. The stem cell culture supernatant and elastin are mixed in syringe 21 to generate a mixed liquid. One end of tube T is connected to the tip of syringe 21. Piston 22 pushes the mixed liquid in syringe 21 toward tube T.

[0024] The introducer 3 has a grip part 31 and a head 32. The grip part 31 is gripped by a user when the introducer 3 is used. The head 32 is provided at the tip of the grip part 31. The head 32 is made of titanium-vanadium. The head 32 is capable of outputting electromagnetic waves (radio waves) of 30 MHz to 300 MHz. The head 32 is provided with a plurality of micro nozzles 32A. The mixed liquid that flows from the mixer 2 into the introducer 3 via the tube T is pressurized inside the head 32. The pressurized mixed liquid is sprayed to the outside from the plurality of micro nozzles 32A.

[0025] The control device 4 is connected to the introducer 3 via a cable C. The control device 4 controls the introducer 3 to output electromagnetic waves from the head 32 and to spray the mixed liquid from the multiple micro nozzles 32A. The control device 4 has an operation button 41. The operation button 41 is operated by a user when switching the operating state of the introducer 3.

[0026] <Beauty method> With reference to FIG. 2, a beauty method in which a practitioner uses the beauty device 1 to perform a treatment on a subject will be described.

[0027] First, the practitioner seals stem cell culture supernatant and elastin in the syringe 21 of the mixer 2 (S11). The stem cell culture supernatant and elastin are mixed in the syringe 21 to generate a mixed liquid (S13). Next, the practitioner operates the piston 22 of the mixer 2 to supply the mixed liquid in the syringe 21 to the introducer 3 (S15). The control device 4 controls the introducer 3 to increase the pressure of the mixed liquid in the head 32 until the practitioner presses the operation button 41 to start the treatment (S17: NO).

[0028] The practitioner grasps the gripping portion 31 of the introducer 3 and presses the head 32 against the skin of the subject. In this state, the practitioner presses the operation button 41 of the control device 4 to start treatment.

[0029] When the control device 4 detects that the operation button 41 has been pressed (S17: YES), the control device 4 causes the mixed liquid whose pressure has been increased in the head 32 to be sprayed from the multiple micro nozzles 32A (S19). At the same time, the control device 4 starts outputting electromagnetic waves from the head 32 (S19).

[0030] The high pressure mixture sprayed from the multiple micro nozzles 32A penetrates the subject's skin to a depth of about 0.1 mm to 0.5 mm from the skin surface, and the mixture is introduced into the basal layer in this area. In addition, the electromagnetic waves output from the head 32 vibrate the subject's skin and heat it up. The thermal effect of this heat promotes the introduction of the mixture into the basal layer. The above operation continues until the practitioner presses the operation button 41 of the control device 4 again (S21: NO).

[0031] When the practitioner wishes to complete the treatment on the subject, he or she presses the operation button 41 of the control device 4 to end the treatment. When the control device 4 detects that the operation button 41 has been pressed (S21: YES), it stops the output of electromagnetic waves from the head 32 and stops the spraying of the mixed liquid from the multiple micro nozzles 32A (S23). This ends the cosmetic method.

[0032] <Actions and Effects of the Present Embodiment> 3 and 4 respectively show the condition of the skin before and after treatment using the cosmetic device 1. As described above, it has been clarified that the condition of the skin is improved by performing treatment using the cosmetic device 1. As described above, the cosmetic device 1 can mix the stem cell culture supernatant and elastin using the mixer 2 and introduce the mixture into the skin using the introducer 3. Therefore, the cosmetic device 1 can improve the condition of the skin and prevent aging.

[0033] The mixture produced by mixing the stem cell culture supernatant and elastin in the mixer 2 is applied to the skin immediately after mixing, more specifically, within 15 to 30 minutes after mixing. In this case, the effects of improving the skin condition and preventing aging can be enhanced compared to when a mixture produced in advance is used.

[0034] As the stem cell culture supernatant, a human-derived stem cell culture supernatant is preferably used. In this case, the affinity of the mixed liquid to the skin can be increased compared to when a stem cell culture supernatant derived from an animal other than human or a plant is used, so that the cosmetic device 1 can improve the effects of improving the skin condition and preventing aging. Also, a stem cell culture supernatant derived from an umbilical cord or amniotic membrane is used as the stem cell culture supernatant. In this case, the cosmetic device 1 can improve the effects of improving the skin condition and preventing aging compared to when a stem cell culture supernatant derived from another origin is used.

[0035] As the elastin, elastin containing cross-linked amino acids (desmosine and isodesmosine) is preferably used. Furthermore, as the elastin, high-purity elastin containing 1.0% or more of cross-linked amino acids (desmosine and isodesmosine) is preferably used. In this case, the beauty device 1 can improve the effect of improving the skin condition and preventing aging, compared to when elastin not containing cross-linked amino acids is used.

[0036] The head 32 of the introducer 3 sprays the mixed liquid at high pressure from a plurality of minute nozzles 32A. In addition, the electromagnetic waves (radio waves) output from the head 32 vibrate and heat the skin of the subject. In this case, the efficiency of introducing the mixed liquid into the basal layer of the skin can be increased, so that the cosmetic device 1 can improve the effects of improving the condition of the skin and preventing aging.

[0037] The effects of treatment using the beauty device 1 are not limited to improving the condition of the skin and preventing aging, but also include various other effects. For example, when the beauty device 1 is used to treat facial skin, effects such as whitening, firmness and luster of the skin, improvement of permanent blemishes, improvement of acne and acne scars, reduction of craters, reduction of sagging and wrinkles, and suppression of inflammation can be achieved. Furthermore, when the beauty device 1 is used to treat body skin, effects such as breast enhancement, elimination of stretch marks, whitening, and elimination of pigmentation can be achieved. Furthermore, when the beauty device 1 is used to treat the scalp, effects such as hair growth and hair thickening, improvement of hair quality, prevention of gray hair, and improvement of the scalp environment can be achieved.

[0038] <Modification> The present invention is not limited to the above-described embodiment, and various modifications are possible. The cosmetic device 1 may appropriately mix other substances in addition to the stem cell culture supernatant and elastin to produce a mixed liquid.

[0039] For example, the cosmetic device 1 may generate a mixed solution by mixing an extracellular matrix (ECM) protein in addition to the stem cell culture supernatant and elastin. Examples of ECM proteins that may be used include agrin, filaggrin, mucin, secretory phosphoprotein 24 (bone matrix), nidogen, cadherin, clathrin, collagen, defensin, entactin, fibrillin, fibronectin, vitronectin, keratin, laminin, microtubule actin cross-linking factor 1, SPARC-like protein, nesprin (nesprin 1, nesprin 2, nesprin 3), fibrous sheath-interacting protein, myomesin, nebulin, keratinocyte proline-rich protein, plakophilin, integrin, talin, exportin, transportin, tenascin, perlecan, sortilin-related receptor, tensin, titin, and the like. The cosmetic device 1 may generate a mixed solution by mixing hyaluronic acid in addition to the stem cell culture supernatant and elastin.

[0040] Furthermore, for example, in addition to the stem cell culture supernatant and elastin, a mixed liquid may be produced by mixing at least one of the following: moisturizers, lower alcohols, vitamins, anti-inflammatory agents, thickeners, gelling agents, pH adjusters, wrinkle improvement agents, various extracts, activators, blood circulation promoters, antiseborrheic agents, anti-inflammatory agents, water-soluble polymers, preservatives, oils (oily substances), film-forming agents, surfactants, oil-soluble gelling agents, colorants, UV absorbers, antibacterial agents, fragrances, antioxidants, chelating agents, emulsifiers, emulsion stabilizers, herbal medicine ingredients, pigments, and whitening agents.

[0041] The mixer 2 of the cosmetic device 1 is not limited to including the syringe 21 and the piston 22 as in the above embodiment. A plurality of microneedles may be provided in the head 32 of the introducer 3 of the cosmetic device 1 instead of the plurality of micronozzles 32A. The mixed solution produced by the mixer 2 may be introduced into the basal layer by piercing the skin with the plurality of microneedles.

[0042] <Other> The steps S11 and S13 are an example of a "mixing step" of the present invention. The step S19 is an example of an "introduction step" of the present invention. [Explanation of symbols]

[0043] 1: Beauty device 2: Mixer 3:Introducer 32: Head 32A: Micro nozzle

Claims

1. A mixer for mixing the stem cell culture supernatant and elastin; The mixture of the stem cell culture supernatant and the elastin is introduced into the skin by an introducer. A beauty device comprising:

2. 2. The cosmetic device according to claim 1, wherein the stem cell culture supernatant is a human stem cell culture supernatant.

3. 2. The cosmetic device according to claim 1, wherein the stem cell culture supernatant is an umbilical cord-derived or amniotic membrane-derived stem cell culture supernatant.

4. The introducer comprises: The cosmetic device according to claim 1, wherein the mixed solution is introduced into the skin within 15 to 30 minutes after the stem cell culture supernatant and elastin are mixed by the mixer.

5. 2. The cosmetic device according to claim 1, wherein the elastin contains desmosine and isodesmosine.

6. The introducer comprises: The cosmetic device according to claim 1, further comprising a head having a plurality of minute nozzles for spraying the mixed liquid.

7. 7. The cosmetic device according to claim 6, wherein the head is capable of outputting electromagnetic waves of 30 MHz to 300 MHz.

8. A mixing step of mixing stem cell culture supernatant with elastin; an introduction step of introducing the mixture of the stem cell culture supernatant and the elastin obtained in the mixing step into the skin; A beauty method comprising the steps of:

9. 9. The cosmetic method according to claim 8, wherein the stem cell culture supernatant is a human stem cell culture supernatant.

10. 9. The cosmetic method according to claim 8, wherein the human stem cell culture supernatant is an umbilical cord-derived or amniotic membrane-derived stem cell culture supernatant.

11. In the introduction step, The cosmetic method according to claim 8, wherein the mixed solution is introduced into the skin within 15 to 30 minutes after the stem cell culture supernatant and elastin are mixed in the mixing step.

12. 9. The cosmetic method according to claim 8, wherein the elastin contains desmosine and isodesmosine.

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