Collagen production promoter

Abstract

To provide a collagen production promoter.SOLUTION: A collagen production promoter contains nicotinamide and decomposed soybean protein.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a collagen production promoter and a product for promoting collagen production. [Background technology]

[0002] Collagen is a major component of the skeletal structure of biological tissues and is widely distributed in skin, cartilage tissue, heart, liver, etc. Collagen also plays a role as a regulator of cell function, so a decrease in collagen can cause various diseases such as joint disorders such as rheumatism and arthritis, and inflammatory diseases.

[0003] In the skin dermis extracellular matrix, collagen fibers form mesh-like bundles to maintain tissue morphology. As collagen fibers mature and proliferate, and cross-linking progresses, the fiber bundles become thicker, providing appropriate firmness to the skin. In aged skin, as fibroblast activity (e.g., collagen production activity) declines, collagen fibers in the dermis extracellular matrix decrease significantly, and abnormal aging cross-links are formed, causing stiffness and losing the inherent elastic firmness. As a result, wrinkles and sagging occur in the skin.

[0004] In order to improve the condition caused by collagen loss, various collagen synthesis promoters have been found. A preparation containing three amino acids consisting of glycine, proline and alanine (Patent Document 1) is known. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Japanese Patent Application Publication No. 7-194375 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide a collagen production promoter containing nicotinamide and a soy protein hydrolysate. [Means for solving the problem]

[0007] The present inventors have found that a composition containing (A) nicotinamide and (B) soy protein hydrolysate has a collagen production promoting effect and significantly promotes collagen production. They have also found that the coexistence of (A) nicotinamide and (B) soy protein hydrolysate can improve skin conditions (wrinkles, firmness, dullness). The present invention has been completed based on these findings.

[0008] That is, the present invention provides [1] A collagen production promoter comprising (A) nicotinamide and (B) a soy protein hydrolysate as active ingredients; [2] The collagen production promoter according to [1], further comprising (C) a plant extract (excluding the above-mentioned (B)) as an active ingredient; [3] The collagen production promoter according to [2], wherein the (C) plant extract (excluding the (B)) is at least one selected from the group consisting of Gynostemma pentaphyllum extract, aloe extract, Rosa rosa extract, Hypericum perforatum extract, Pueraria lobata extract, cherry leaf extract, Tilia extract, Horsetail extract, Citrus unshiu extract, Calendula officinalis extract, neem leaf extract, hop extract, evening primrose extract, centaurea cyanus extract, Coix seed extract, lemon extract, rosemary extract, Roman chamomile extract, royal jelly extract, chamomilla recutita extract, carrot extract, Geranium globulus extract, bilberry leaf extract, and Horse Chestnut extract; [4] The collagen production-promoting agent according to [2] or [3], wherein the parts by mass of (B) the soybean protein hydrolysate and (C) the plant extract (excluding (B)) per part by mass of (A) nicotinamide are 0.001 to 0.25; [5] A composition for external use for promoting collagen production, comprising (A) nicotinamide and (B) a soy protein hydrolysate; [6] The composition for external use for promoting collagen production according to [5], further comprising (C) a plant extract (excluding the above-mentioned (B)) as an active ingredient; [7] The composition for external use for promoting collagen production according to [6], wherein the (C) plant extract (excluding the (B)) is at least one selected from the group consisting of Gynostemma pentaphyllum extract, aloe extract, Rosa roxburghii extract, Hypericum perforatum extract, Pueraria lobata extract, cherry leaf extract, Tilia extract, Equisetum arvense extract, Citrus chinensis extract, Calendula officinalis extract, neem leaf extract, hop extract, evening primrose extract, centaurea cyanus extract, Coix seed extract, lemon extract, rosemary extract, Roman chamomile extract, royal jelly extract, chamomilla recutita extract, carrot extract, Geranium globulus extract, bilberry leaf extract, and Horse Chestnut extract; [8] The composition for external use for promoting collagen production according to [6] or [7], wherein the parts by mass of (B) the soy protein hydrolysate and (C) the plant extract (excluding (B)) per part by mass of (A) nicotinamide are 0.001 to 0.25; [9] A food or beverage composition for promoting collagen production, comprising (A) nicotinamide and (B) a soy protein hydrolysate;

[10] A cosmetic method for improving and / or preventing a condition caused by a decrease in collagen in the skin by applying to the skin a composition containing (A) nicotinamide and (B) a soy protein hydrolysate (excluding methods for treating human diseases); etc. Effect of the Invention

[0009] The collagen production promoter of the present invention contains nicotinamide and a soy protein hydrolysate, and therefore can promote collagen production. In addition, the collagen production promoter of the present invention contains nicotinamide and a soy protein hydrolysate, and therefore is excellent in improving and / or preventing conditions caused by a decrease in collagen in the skin (skin wrinkles, loss of skin elasticity, skin dullness, etc.). [Brief description of the drawings]

[0010] [Figure 1] 1 shows the results of collagen production by dermis-derived fibroblasts in Test Example 1. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0011] Hereinafter, an embodiment of the present invention will be described in detail. However, the present invention is not limited to the following embodiment.

[0012] [1. Collagen production promoter] The collagen production promoter according to this embodiment contains nicotinamide and a soy protein hydrolysate.

[0013] The content of nicotinamide in the collagen production promoter according to this embodiment is not particularly limited as long as it has a collagen production promoting effect, and is appropriately set according to the type and content of other blended components, the formulation form of the collagen production promoter, etc. From the viewpoint of more significantly exhibiting the effects of the present invention, for example, it is preferably 0.01 to 20 mass%, more preferably 0.05 to 15 mass%, even more preferably 0.1 to 10 mass%, and particularly preferably 0.1 to 8 mass%. The dosage to humans is preferably 0.01 to 100 mg per day, more preferably 0.05 to 90 mg, even more preferably 0.1 to 80 mg, and particularly preferably 1 to 60 mg. Nicotinamide synthesized by a known method or commercially available products can also be used.

[0014] The soy protein hydrolysate in the collagen production promoter according to the present embodiment is not particularly limited as long as it is derived from soybean of the legume family, and one or more soybean plants may be selected and used. The plant parts used for the soy protein hydrolysate may be any parts, and one or more parts may be selected and used. The extraction method and extraction solvent for the soy protein hydrolysate are not particularly limited, and known techniques can be used. The decomposition treatment method for the soy protein hydrolysate is not particularly limited, and known techniques can be used. The soy protein hydrolysate according to the present embodiment may be a commercially available product. The content of the soy protein hydrolysate in the collagen production promoter according to the present embodiment is not particularly limited as long as it is within a range that has a collagen production promoting effect, and is appropriately set according to the type and content of other blended ingredients, the formulation form of the collagen production promoter, and the like. From the viewpoint of more significantly exhibiting the effects of the present invention, for example, it is preferably 0.00001 to 2% by mass, more preferably 0.0001 to 1% by mass, even more preferably 0.0005 to 0.5% by mass, and particularly preferably 0.001 to 0.1% by mass. The dosage to humans is preferably 0.001 to 100 mg, more preferably 0.01 to 80 mg, even more preferably 0.1 to 60 mg, and preferably 1 to 50 mg per day.

[0015] The plant extract (excluding the above-mentioned (B) soy protein hydrolysate) in the collagen production promoter according to this embodiment is not particularly limited as long as it is derived from a plant. Any part of the plant may be used for the plant extract, and one or more parts may be selected and used. The extraction method and extraction solvent for the plant extract are not particularly limited, and known techniques can be used. The plant extract may be subjected to a decomposition treatment, and the decomposition treatment method is not particularly limited, and known techniques can be used. The plant extract according to this embodiment can also be a commercially available one. From the viewpoint of more prominently exhibiting the effect of the present invention, it is preferable to use one or more plant extracts selected from the group consisting of Gynostemma pentaphyllum extract, aloe extract, Rosa rosa extract, Hypericum perforatum extract, Pueraria lobata extract, Cherry leaf extract, Tilia extract, Horsetail extract, Citrus arvense extract, Calendula officinalis extract, Neem leaf extract, Hop extract, Oenothera biennis extract, Cornflower extract, Coix seed extract, Lemon extract, Rosemary extract, Roman chamomile extract, Royal jelly extract, Chamomilla recutita extract, Carrot extract, Geranium globulus extract, Bilberry leaf extract and Horse chestnut extract. The content of the plant extract in the collagen production promoter according to this embodiment is not particularly limited as long as it has a collagen production promoting effect, and is appropriately set according to the type and content of other blended ingredients, the formulation form of the collagen production promoter, etc. From the viewpoint of more prominently exhibiting the effects of the present invention, for example, the concentration is preferably 0.00001 to 5% by mass, more preferably 0.0001 to 3% by mass, even more preferably 0.0005 to 2% by mass, and particularly preferably 0.001 to 1% by mass. The dosage to humans is preferably 0.001 to 200 mg per day, more preferably 0.01 to 150 mg, even more preferably 0.1 to 100 mg, and preferably 1 to 80 mg.

[0016] The parts by mass of soy protein hydrolysate and plant extract (excluding the soy protein hydrolysate) per part by mass of nicotinamide in the collagen production promoter of this embodiment is not particularly limited, but from the viewpoint of more significantly exhibiting the effects of the present invention, it is, for example, preferably 0.0001 to 10, more preferably 0.005 to 5, even more preferably 0.001 to 2.5, and particularly preferably 0.002 to 1.5.

[0017] The collagen production promoter of the present invention is expected to be effective in preventing and / or improving conditions caused by a decrease in collagen in the skin, such as wrinkles, loss of firmness and elasticity, and dullness. In particular, since the face is prone to changes in impression due to changes in appearance associated with aging, it is expected to be effective in preventing and / or improving hollows in the eyelids, sagging and puffy tear bags and eye bags, wrinkles at the corners of the eyes, wrinkles on the forehead and between the eyebrows, wrinkles around the mouth (nasolabial folds), etc. In particular, the prevention and / or improvement of the formation of deep wrinkles is expected to be highly effective in preventing and / or improving changes in the appearance impression. Although any part may be used,

[0018] The collagen production promoter according to this embodiment may further contain excipients, thickeners, binders, disintegrants, dispersants, surfactants, suspending agents, emulsifiers, stabilizers, gelling agents, sweeteners, sour agents, umami agents, salty agents, bitter agents, colorants, UV protection agents, antioxidants, preservatives, antibacterial agents, pH adjusters, buffers, chelating agents, vitamins, flavorings, additives such as minerals, amino acids or salts thereof, sugars, moisturizers, organic acids or salts thereof, dietary fiber, biological extracts (excluding plant extracts), etc. These can be appropriately selected from known ones and can be produced using conventional methods.

[0019] The dosage form of the collagen production promoter according to the present embodiment can be used without being limited to a specific product or property, and can be appropriately prepared by a known method in any dosage form, such as liquid, gel, cream, paste, solid, aerosol, or patch. Examples of liquid forms include lotion, milky lotion, beauty essence, oral liquid, and syrup. Examples of solid forms include stick, tablet, chewable, granule, powder, capsule, and ointment. In addition, the dosage form may be either an external or internal agent, but from the viewpoint of achieving the effects of the present invention, an external agent is preferable. The external agent may be either a pharmaceutical or a cosmetic, and is not limited to these. These can be manufactured using conventional methods.

[0020] 2. Products for promoting collagen production The topical product according to this embodiment has a collagen production promoting effect. Thus, as one embodiment of the present invention, there is provided a topical product for promoting collagen production, which contains (A) nicotinamide and (B) a soy protein hydrolysate.

[0021] In this embodiment, the contents of nicotinamide, soy protein hydrolysate, and plant extract, the types and contents of other ingredients, the formulation form and use of the external product, etc. are as explained in [1. Collagen production promoter].

[0022] [3. Cosmetic method for promoting collagen production] The cosmetic method according to the present embodiment has a collagen production promoting effect. Therefore, as one embodiment of the present invention, there is provided a cosmetic method (excluding methods for treating human diseases) in which a topical composition containing (A) nicotinamide and (B) soy protein hydrolysate is applied to the skin to improve and / or prevent conditions caused by a decrease in collagen in the skin.

[0023] In this embodiment, the contents of nicotinamide, soy protein hydrolysate, and plant extract, the types and contents of other ingredients, the formulation form and use of the external product, etc. are as explained in [1. Collagen production promoter]. EXAMPLES

[0024] The present invention will be specifically described below based on test examples, but the present invention is not limited to these.

[0025] [Test Example 1: Collagen Production Evaluation] 2 × 10 normal human skin fibroblasts (KURABO KF-4009) 4 Cells were seeded in a 96-well plate at 1000 cells / well and incubated at 37℃, 5% CO 2 After confirming under a microscope that there were no problems with cell growth, each component was added to the medium so that the final concentration of each component was as shown in Table 1, and the medium was incubated at 37°C and 5% CO 2 The cells were cultured for 3 days under the above conditions. The culture supernatant was collected, and the amount of type I collagen was measured using an enzyme-linked immunosorbent assay (Anti-Human Procollagen typeI C-peptide EIA Kit; manufactured by Takara Bio Inc.), and the concentration was calculated. A test solution containing no nicotinamide or soy protein hydrolysate was used as a control, and the amount of collagen in each test solution was calculated assuming that the amount of type I collagen in the control was 100. The results are shown in FIG. 1.

[0026] [Table 1]

[0027] As shown in FIG. 1, the test solution containing nicotinamide and soy protein hydrolysate showed a significant increase in collagen production compared to the test solution containing nicotinamide only or the test solution containing soy protein hydrolysate only.

[0028] [Test Example 2: Skin Evaluation] Formulation examples 1, 2, and 3 were prepared using known techniques. Five subjects applied Formulation example 1 to the forehead, between the eyebrows, under the eyes, corners of the eyes, and around the mouth on the right side of the face, and Formulation example 2 to the left side of the face twice a day for three days. The difference in skin condition between the left and right sides of the face after three days and the change in skin condition before and after three days of application were evaluated. Next, the evaluation was performed in the same manner except that Formulation example 2 was changed to Formulation 3.

[0029] Prescription Example 1 Nicotinamide 5.0 (mass%) Hydrolyzed Soy Protein 0.1 Glycerin 7.0 1,3-Butylene glycol 3.0 Squalane 5.0 Polyglyceryl Monostearate 3.0 Tocopherol 0.2 Carboxyvinyl polymer 0.1 Triethanolamine (appropriate amount) Distilled water remaining Total 100.0%

[0030] Prescription Example 2 Nicotinamide 5.0 (mass%) Hydrolyzed Soy Protein 0.1 Acetylated hyaluronic acid 0.1 Jiaogulan extract 0.1 Aloe extract 0.05 Rosa roxburghii extract 0.05 Hypericum extract 0.002 Pueraria lobata extract 0.01 Cherry leaf extract 0.1 Linden extract 0.001 Horsetail extract 0.001 Chinpi extract 0.1 Calendula officinalis extract 0.005 Neem Leaf Extract 0.005 Hops extract 0.004 Evening Primrose Extract 0.01 Cornflower extract 0.02 Coix seed extract 0.05 Lemon extract 0.002 Rosemary extract 0.01 Roman chamomile extract 0.05 Royal jelly extract 0.08 Glycerin 7.0 1,3-Butylene glycol 3.0 Squalane 5.0 Polyglyceryl Monostearate 3.0 Tocopherol 0.2 Carboxyvinyl polymer 0.1 Triethanolamine (appropriate amount) Distilled water remaining Total 100.0%

[0031] Prescription Example 3 Nicotinamide 5.0 (mass%) Hydrolyzed Soy Protein 0.1 Hydrolyzed silk 0.004 Chamomilla extract 0.5 Carrot extract 0.01 Geranium extract 0.01 Bilberry Leaf Extract 0.05 Horse Chestnut Extract 0.01 Glycerin 7.0 1,3-Butylene glycol 3.0 Squalane 5.0 Polyglyceryl Monostearate 3.0 Tocopherol 0.2 Carboxyvinyl polymer 0.1 Triethanolamine (appropriate amount) Distilled water remaining Total 100.0%

[0032] For Formulation Example 1, five subjects evaluated that the skin was firmer (more elastic) in all areas of the face, including the forehead, between the eyebrows, under the eyes, corners of the eyes, and around the mouth, three days after the evaluation than before the evaluation started. Three out of five subjects evaluated that the small wrinkles at the corners of the eyes and under the eyes were shallower and less noticeable three days after the evaluation than before the evaluation started. For Formulation Example 2, five subjects evaluated that the skin was firmer (more elastic) in all areas of the face, including the forehead, between the eyebrows, under the eyes, corners of the eyes, and around the mouth, three days after the evaluation than before the evaluation started. Four out of five subjects evaluated that the deep wrinkles at the forehead and between the eyebrows were less noticeable three days after the evaluation than before the evaluation started. Three out of five subjects evaluated that the skin condition of the left and right sides of the face after three days was improved and looked brighter on the left side of the face where Formulation Example 2 was applied, compared to the right side where Formulation Example 1 was applied. Regarding the skin condition 12 hours after applying liquid foundation after applying Formulation 1 and Formulation 2, three out of five subjects reported that the left side of the face where Formulation 2 was applied showed improvement in the makeup smear caused by the liquid foundation getting into the nasolabial folds. For Formulation 3, five subjects evaluated that the skin was firmer (more elastic) in all areas of the face, including the forehead, between the eyebrows, under the eyes, corners of the eyes, and around the mouth, three days after the evaluation compared to before the evaluation began. Three out of five subjects evaluated that the small wrinkles at the corners of the eyes and under the eyes were shorter and less noticeable on the left side of the face where Formulation 3 was applied, compared to the right side where Formulation 1 was applied.

Claims

[Claim 1] The invention described herein.

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