Composition for improving insulin resistance

Abstract

To provide a method and means for improving insulin resistance.SOLUTION: The present invention relates to a composition for improving insulin resistance, the composition containing turmeric extract as an active ingredient.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a composition for improving insulin resistance. The present invention also relates to a composition for treating, preventing or improving a disease or disorder associated with insulin resistance. [Background technology]

[0002] Insulin is secreted into the blood from the pancreas after a meal and acts on skeletal muscle cells and fat cells, which take up glucose and lower blood sugar levels. When glucose metabolism disorders occur due to aging or an irregular lifestyle such as overeating, the effect of insulin to lower blood sugar levels weakens, and as a result, it is known that blood sugar levels increase after meals and that elevated blood sugar levels are less likely to return to normal. This state in which insulin becomes less effective is called insulin resistance. If this condition is left untreated and insulin becomes even less effective, type 2 diabetes will develop. In other words, in order to prevent type 2 diabetes, it is important to suppress the increase in blood sugar levels after meals.

[0003] It has been reported that blood glucose levels improved in people who took turmeric extract (Patent Document 1). On the other hand, there are reports that in people who took turmeric, the increase in postprandial insulin levels was promoted, but no change was observed in blood glucose levels (Non-Patent Document 1), and that no change was observed in postprandial blood glucose or insulin levels (Non-Patent Document 2). There are also reports that in people who took curcumin, a component contained in turmeric, no change was observed in postprandial blood glucose levels (Non-Patent Document 3), and that the increase in postprandial blood glucose levels was suppressed (Non-Patent Document 4). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Patent Publication No. 2021-8434 [Non-patent literature]

[0005] [Non-Patent Document 1] Wickenberg, et al., Nutr J. 9:43, 2010 [Non-Patent Document 2] Lee, et al., Asia Pac J Clin Nutr. 23(4):581-91, 2014 [Non-Patent Document 3] Alwi, et al., Acta Med Indonesia. 40(4):201-10, 2008 [Non-Patent Document 4] Thota, et al., Sci Rep. 8(1):13679, 2018 Summary of the Invention [Problem to be solved by the invention]

[0006] Insulin resistance may be a factor in increasing the risk of various diseases other than blood glucose-related diseases. Therefore, the present invention aims to provide a method and means for improving insulin resistance. Another object of the present invention is to provide a method and means for preventing, treating, or ameliorating diseases or disorders associated with insulin resistance. [Means for solving the problem]

[0007] In the course of intensive research into the effects of turmeric extract, the present inventors discovered that turmeric extract has the effect of improving insulin resistance because it suppresses an increase in blood glucose level without promoting insulin secretion, and gained the knowledge that turmeric extract can improve insulin resistance and / or ameliorate diseases or disorders associated with insulin resistance, thereby completing the present invention.

[0008] Thus, the present invention encompasses, for example: [1] A composition for improving insulin resistance, comprising turmeric extract as an active ingredient. [2] A composition for treating, preventing, or ameliorating a disease or disorder associated with insulin resistance, comprising turmeric extract as an active ingredient. [3] The composition described in [1] or [2], wherein the composition consists solely of turmeric extract as an active ingredient. [4] The composition according to any one of [1] to [3], wherein the turmeric extract comprises a turmeric extract using at least one extraction solvent selected from the group consisting of water and hydrophilic organic solvents. [5] The composition according to any one of [1] to [3], wherein the turmeric extract comprises a mixture of a turmeric extract obtained by extraction with water and a turmeric extract obtained by extraction with supercritical carbon dioxide. [6] The composition described in any of [1] to [5], wherein the turmeric extract contains at least one of turmeronol A, turmeronol B, and bisacronol.

[0009] [7] Use of turmeric extract for producing a composition for improving insulin resistance. [8] Use of a turmeric extract for the manufacture of a composition for treating, preventing, or ameliorating a disease or disorder associated with insulin resistance.

[0010] [9] administering a turmeric extract to a subject in need of improving insulin resistance; and improving insulin resistance in said subject. 23. A method for improving insulin resistance in a subject, comprising:

[10] administering a turmeric extract to a subject in need of treatment, prevention, or amelioration of a disease or disorder associated with insulin resistance; and Treating, preventing, or ameliorating a disease or disorder associated with insulin resistance in said subject. 23. A method for treating, preventing, or ameliorating a disease or disorder associated with insulin resistance in a subject, comprising:

[0011]

[11] Turmeric extract for use in improving insulin resistance.

[12] A turmeric extract for use in the treatment, prevention, or amelioration of a disease or disorder associated with insulin resistance. Effect of the Invention

[0012] According to one or more embodiments of the present invention, there is provided a composition for improving insulin resistance. In accordance with one or more further embodiments of the present invention, there is provided a composition for preventing, treating, or ameliorating a disease or disorder associated with insulin resistance. [Brief description of the drawings]

[0013] [Figure 1] This is a graph showing the change in blood glucose level before glucose loading (0 minutes) and 30 minutes, 60 minutes, and 120 minutes after glucose loading in the control group that took turmeric extract and the placebo group. ** indicates a significant difference of p<0.01 compared to the placebo group. [Diagram 2] This is a graph showing the change in insulin levels before glucose loading (0 minutes) and 30, 60, and 120 minutes after glucose loading in the control group that took turmeric extract and the placebo group. ** indicates a significant difference of p<0.01 compared to the placebo group. [Diagram 3] 1 is a graph showing the Cederholm index (an index of insulin sensitivity) calculated from blood glucose and insulin levels in an OGTT test in a control group that took turmeric extract and a placebo group. * indicates a significant difference of p<0.05 compared to the placebo group. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0014] The present invention is based on the finding that insulin resistance can be improved by administering or ingesting turmeric extract. Specifically, the present invention is based on the experimental results that in humans, ingesting turmeric extract suppresses the rise in blood glucose level after a meal and, although insulin secretion was not promoted, a decrease in insulin level after a drop in blood glucose level was observed, and thus the intake of turmeric extract improved the effectiveness of insulin (i.e., improved insulin resistance).

[0015] Therefore, in one aspect, the present invention provides a composition for improving insulin resistance, which contains turmeric extract as an active ingredient.

[0016] Insulin resistance refers to a state in which sensitivity to insulin, a hormone that has a blood glucose lowering effect, is reduced, and insulin cannot fully exert its action. Specifically, it refers to a state in which, despite the secretion of insulin from the pancreas into the blood, the sensitivity of target organs (skeletal muscle, adipose tissue, liver) to insulin is reduced, and its action (glucose uptake in skeletal muscle and adipose tissue, inhibition of gluconeogenesis in the liver) is weakened. On the other hand, when the amount of insulin produced by the pancreas decreases, the amount of insulin secreted decreases, and as with insulin resistance, blood glucose levels rise and the risk of diabetes increases. However, this state in which the amount of insulin produced by the pancreas decreases is called insulin secretion disorder (decreased insulin secretion), and is a state that is distinguished from insulin resistance.

[0017] Insulin resistance can be evaluated by measuring blood glucose levels (postprandial blood glucose levels) and insulin levels after a sugar load. Since blood insulin levels increase rapidly after a sugar load, the effect of insulin is greater than that of fasting blood glucose levels without a sugar load (which are controlled by glucagon, the liver, and the like in addition to insulin), and this can be used as an evaluation index for insulin resistance. Blood glucose concentration (blood glucose level) and insulin levels after a sugar load are measured over time, and if insulin levels are higher than normal due to excessive promotion of insulin secretion to lower blood glucose levels, or if blood glucose levels are high despite normal insulin levels, the patient can be evaluated as having insulin resistance. In addition, the Cederholm index is known as one of the indicators of insulin sensitivity (Cederholm, J. and Wibell, L. Diabetes Res Clin Pract. 10(2):167-75, 1990; Radikova, Z. Endocr Regul. 37(3):189-94, 2003), and the higher the value of this Cederholm index, the higher the insulin sensitivity, and the lower the value, the lower the insulin sensitivity. When the value of the Cederholm index is low, it can be evaluated that the insulin resistance is high.

[0018] Improvement of insulin resistance means, compared to when turmeric extract is not administered or taken, suppressing the decrease in insulin action, increasing the decreased insulin action or bringing it closer to normal, making the change from increase to decrease in insulin level after glucose loading normal or bringing it closer to normal, or increasing the Cederholm index value or bringing it closer to normal.

[0019] <Composition containing turmeric extract> The composition according to the present invention contains a turmeric extract. In the present invention, the turmeric extract refers to an extract (turmeric extract) of a plant material derived from a plant of the genus Curcuma of the Zingiberaceae family using an extraction solvent. The turmeric extract is not limited to a solvent extract obtained by extraction with an extraction solvent, but also includes a solvent extract that has been fractionated and purified by column chromatography or the like. The turmeric extract used in the present invention can be in the form of an extract liquid after the completion of an extraction operation (including a fractionation and purification operation when fractionation and purification is performed), a concentrate obtained by partially removing the solvent from the extract liquid, or a dried product obtained by removing the solvent from the extract liquid. The removal of the solvent from the extract can be carried out by volatilizing the solvent by heating and / or reducing pressure, etc. The method of heating and reducing pressure is not particularly limited, and for example, a conventionally known method can be used.

[0020] The plant raw material may be the rhizome of Curcuma longa, Curcuma aromatica, Curcuma zedoaria, Curcuma phaeocaulis, Curcuma kwangsiensis, Curcuma wenyujin, and / or Curcuma xanthorrhiza, which are plants of the Zingiberaceae family, and the rhizome of Curcuma longa is particularly preferred. The rhizome may be collected from the ground, and an appropriate part of the rhizome may be used as is, or cut to an appropriate size or shape, or in the form of a pulverized product. These plant raw materials may be appropriately dried.

[0021] As the extraction solvent, at least one selected from the group consisting of water and hydrophilic organic solvents can be used. As the at least one extraction solvent selected from the group consisting of water and hydrophilic organic solvents, any of water, hydrophilic organic solvents, and mixed solvents of water and hydrophilic organic solvents can be used. The hydrophilic organic solvent can be a mixed solvent of multiple hydrophilic organic solvents. "Water" also includes hot water. As the hot water, for example, hot water of 95°C or higher can be used. As the hydrophilic organic solvent, at least one alcohol (which may be a mixed solvent of multiple alcohols) can be mentioned, and the alcohol is not particularly limited, but ethanol is preferable. When a mixed solvent of alcohol and water is used as the extraction solvent, the mixing ratio is not particularly limited, but for example, a range of 10:90 to 90:10 by weight ratio is preferable, and a range of 20:80 to 50:50 is more preferable. In addition, supercritical carbon dioxide can also be used as the extraction solvent.

[0022] The method for extracting turmeric from plant raw materials is not particularly limited, and any extraction method commonly used in the art can be used.

[0023] In the present invention, the turmeric extract may contain one type of turmeric extract, or may contain two or more types of turmeric extracts with different extraction solvents and / or extraction methods. In one embodiment, the turmeric extract contains a mixture of a turmeric extract obtained using at least one selected from the group consisting of water and a hydrophilic organic solvent as an extraction solvent, and a turmeric extract obtained using supercritical carbon dioxide, for example, a mixture of a turmeric extract obtained by extraction with water and a turmeric extract obtained by extraction with supercritical carbon dioxide. The ratio of each extract in the mixture is not particularly limited, and may be, for example, a weight ratio of 1:0.01 to 1:1.

[0024] In a preferred embodiment, the turmeric extract comprises at least one of an extract with water, such as hot water, and an extract with supercritical carbon dioxide.

[0025] The turmeric extract used in the present invention contains at least one of turmeronol A, turmeronol B and bisacronol.

[0026] Turmeronol A and turmeronol B are compounds having the following planar structures:

[0027] [ka]

[0028] [ka]

[0029] In the present invention, turmeronol A and turmeronol B each have the above-mentioned planar structure, and may be any optical isomer or a mixture of multiple optical isomers.

[0030] It is known that the configuration of the 6-carbon atom in the partial structure of 2-methyl-2-hepten-4-one in natural products isolated from turmeric extract is S-configuration for turmeronol A and turmeronol B. However, in the present invention, turmeronol A and turmeronol B may have the above-mentioned planar structure, and the configuration may be S-configuration, R-configuration, or a mixture of S-configuration and R-configuration.

[0031] In the present invention, bisaclone is a compound having the following planar structure: Bisaclone has an asymmetric carbon at the position indicated by the * mark in the planar structural formula and may contain multiple optical isomers, but in the present invention, bisaclone may be any optical isomer or a mixture of two or more optical isomers.

[0032] [ka]

[0033] The turmeric extract may be processed by a conventional method, such as drying, powdering, granulation, or solution.

[0034] <Composition of the Present Invention> One aspect of the composition of the present invention relates to a composition for improving insulin resistance, containing the turmeric extract as an active ingredient. By administering the composition of the present invention according to this aspect to a subject such as a human, insulin resistance in the subject can be improved. Here, the active ingredient is ingested or administered in an effective amount for improving insulin resistance. As the route of administration, oral or nasal administration is preferred, oral or nasal administration is more preferred, and oral administration is particularly preferred. The composition of the present invention can improve insulin resistance by suppressing blood glucose rise in the body (particularly, suppressing blood glucose rise without promoting insulin secretion).

[0035] Another aspect of the composition of the present invention relates to a composition for treating, preventing or improving a disease or disorder associated with insulin resistance, which contains the turmeric extract as an active ingredient. By having a subject such as a human ingest or administer the composition of the present invention according to this aspect, it is possible to treat, prevent or improve a disease or disorder associated with insulin resistance in the subject. Here, the active ingredient is ingested or administered in an effective amount for treating, preventing or improving a disease or disorder associated with insulin resistance. The route of administration is preferably oral or nasal, and more preferably oral.

[0036] A disease or disorder associated with insulin resistance is a disease or disorder caused by insulin resistance, and can be prevented, treated, or ameliorated by improving insulin resistance according to the present invention. Such diseases or disorders include type 2 diabetes, arteriosclerosis, hypertension, heart disease, obesity, metabolically-related fatty liver disease, steatosis, steatohepatitis, cirrhosis, energy homeostasis disorder, brain development disorder, Alzheimer's disease, cancer (breast cancer, uterine cancer, cervical cancer, colon cancer, esophageal cancer, pancreatic cancer, kidney cancer, prostate cancer), hyperfiltration, chronic kidney disease, polycystic ovary syndrome, menstrual abnormality infertility, etc. (Li, M. et al. Signal Transduct Target Ther. 6;7(1):216, 2022).

[0037] The composition of the present invention may be in the form of a drug, food or drink, feed, food additive, feed additive, etc., and is more preferably a drug or food or drink. Food or drink includes functional food, food for specified health use, nutritional supplement, etc. The composition of the present invention is preferably in the form of a composition that is taken or administered orally or nasally, and more preferably in the form of a composition that is taken or administered orally.

[0038] The content of the turmeric extract in the composition of the present invention is preferably such that the total amount of turmeronol A and turmeronol B is 100 μg or more per daily intake or administration of the composition of the present invention, preferably per daily intake or administration for one human, particularly for one adult, and / or the amount of turmeronol A is 17 μg or more, preferably 28 μg or more, more preferably 43 μg or more, even more preferably 57 μg or more, most preferably 80 μg or more, for example 100 μg, and / or the amount of turmeronol B is 5 μg or more. g or more, preferably 8 μg or more, more preferably 12 μg or more, even more preferably 16 μg or more, most preferably 20 μg or more, for example 100 μg, and / or 80 μg or more, preferably 133 μg or more, more preferably 200 μg or more, even more preferably 267 μg or more, most preferably 360 μg or more, for example 400 μg as bisaclone, which is preferable in terms of effectively obtaining the improving effect of insulin resistance and the treating, preventing or improving effect of diseases or disorders related to insulin resistance. The upper limit of the content of the turmeric extract or each component is not particularly limited. Here, the "daily intake or administration amount" is typically 0.1 g to 500 g as the amount of the composition of the present invention when taken or administered orally or nasally, preferably orally. The composition of the present invention may be taken or administered continuously, or may be taken or administered when necessary.

[0039] The composition of the present invention containing the turmeric extract as an active ingredient may be the turmeric extract itself, or may be a composition containing the turmeric extract and at least one other ingredient. When the composition of the present invention contains the turmeric extract and at least one other ingredient, it may be a composition in which the turmeric extract and at least one other ingredient are mixed, or a composition in which the turmeric extract and at least one other ingredient are formulated by an appropriate means, or a composition in which the turmeric extract and at least one other ingredient are further mixed with other ingredients. In one embodiment, the composition of the present invention is a composition consisting of only turmeric extract as an active ingredient. "Consisting only of turmeric extract as an active ingredient" means that the active ingredient exhibiting the desired activity (improvement of insulin resistance) is only turmeric extract, and does not contain any other active ingredient. However, components other than the active ingredient, such as carriers and excipients, may be included in the composition of the present invention.

[0040] The form of the composition of the present invention is not particularly limited, and may be, for example, any form such as liquid, fluid, gel, semi-solid, or solid.

[0041] The at least one other component that may be contained in the composition of the present invention is not particularly limited, but examples thereof include components that are acceptable in the final form of a pharmaceutical product, food or drink, nutritional supplement, supplement, feed, food additive, feed additive, etc., and that can be orally ingested.

[0042] Examples of such other components include sweeteners, acidulants, vitamins, minerals, thickeners, emulsifiers, antioxidants, water, etc. Furthermore, colorants, flavors, preservatives, antiseptics, fungicides, and further physiologically active substances may be added as necessary.

[0043] Examples of sweeteners include monosaccharides and disaccharides such as glucose, fructose, sucrose, lactose, maltose, palatinose, trehalose, and xylose, isomerized sugars (glucose-fructose liquid sugar, fructose-glucose liquid sugar, mixed isomerized sugar, etc.), sugar alcohols (erythritol, xylitol, lactitol, palatinite, sorbitol, reduced starch syrup, etc.), honey, and high-intensity sweeteners (sucralose, acesulfame potassium, thaumatin, stevia, aspartame, etc.).

[0044] The acidulant may be citric acid, malic acid, gluconic acid, tartaric acid, lactic acid, phosphoric acid, or a salt thereof, and one or more of these may be used.

[0045] Examples of vitamins include vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin E, niacin, and inositol.

[0046] Examples of minerals include calcium, magnesium, zinc, and iron.

[0047] Examples of thickening agents include carrageenan, gellan gum, xanthan gum, gum arabic, tamarind gum, guar gum, locust bean gum, karaya gum, agar, gelatin, pectin, soybean polysaccharides, and carboxymethylcellulose (CMC).

[0048] Examples of the emulsifier include glycerin fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, lecithin, plant sterols, and saponin.

[0049] Antioxidants include vitamin C, tocopherol (vitamin E), enzyme-treated rutin, and the like. The other ingredients can be appropriately blended in amounts within the ranges that are normally used by those skilled in the art in compositions such as foods, beverages, and pharmaceuticals.

[0050] The form of a composition prepared by formulating the composition of the present invention and at least one other component by an appropriate means may be in the form of a solid composition such as powder, granules, capsules, tablets (including coated tablets such as sugar-coated tablets or multi-layer tablets, orally disintegrating tablets, chewable tablets, etc.), or in the form of a liquid composition such as a solution.

[0051] In addition, the composition of the present invention is relatively safe and easy to be continuously ingested for a long period of time. Therefore, the composition of the present invention can be preferably used in foods and beverages, nutritional supplements, supplements, and feeds. The composition of the present invention has the above-mentioned functions or activities, and contains turmeric extract that has been ingested in the past, and is relatively safe. Furthermore, it can be added to various foods and beverages and ingested continuously, and is expected to exert the intended functions or actions.

[0052] The composition of the present invention is preferably a composition to be taken continuously, specifically, a composition to be taken continuously once or twice a day, preferably for 4 weeks or more, more preferably for 8 weeks or more, and most preferably for 12 weeks or more.

[0053] <How to improve insulin resistance> A further aspect of the present invention is to administer the turmeric extract to a subject in need of improving insulin resistance; and improving insulin resistance in said subject. The present invention relates to a method for improving insulin resistance, comprising:

[0054] The turmeric extract used in the method according to this embodiment may be in the above-mentioned form of the composition of the present invention.

[0055] The subject in the method according to this embodiment is typically a human, but may be a mammal other than a human.

[0056] In the method according to this embodiment, the administration route is preferably oral or nasal administration, and particularly preferably oral administration.

[0057] In the method according to this embodiment, the dose of the turmeric extract administered to a subject is not particularly limited as long as it is an effective amount for improving insulin resistance. For example, when the subject is an adult, the turmeric extract is preferably administered, based on the components contained therein, so that the total amount of turmeronol A and turmeronol B per adult is 100 μg or more, and / or turmeronol A is 17 μg or more, preferably 28 μg or more, more preferably 43 μg or more, even more preferably 57 μg or more, most preferably 80 μg or more, for example 100 μg, and / or turmeronol B is 5 μg or more, preferably 8 μg or more, more preferably 12 μg or more, even more preferably 16 μg or more, most preferably 20 μg or more, for example 100 μg, and / or bisaclone is 80 μg or more, preferably 133 μg or more, more preferably 200 μg or more, even more preferably 267 μg or more, most preferably 360 μg or more, for example 400 μg.

[0058] The amount of administration or intake of the composition of the present invention varies depending on the age and weight of the subject, the route of administration or intake, the number of times of administration or intake, the purpose of administration (improvement of insulin resistance, prevention, treatment or improvement of diseases or symptoms related to insulin resistance, etc.), and can be changed widely at the discretion of a person skilled in the art so as to achieve the desired action. The proportion of turmeric extract in the composition is not particularly limited, and may be appropriately adjusted according to the ease of production, the preferred daily dose, etc. Turmeric extract is relatively safe, so the intake amount can be further increased. The daily intake amount may be taken in one dose, or may be taken in several doses. The frequency of administration or intake is also not particularly limited, and can be appropriately selected according to various conditions such as the route of administration or intake, the age and weight of the subject, and the desired effect.

[0059] <Method of treating, preventing or ameliorating diseases or disorders associated with insulin resistance> A further aspect of the present invention comprises administering the turmeric extract to a subject in need of treatment, prevention, or amelioration of a disease or disorder associated with insulin resistance; and Treating, preventing, or ameliorating a disease or disorder associated with insulin resistance in said subject. The present invention relates to a method for treating, preventing, or ameliorating a disease or disorder associated with insulin resistance, comprising:

[0060] The turmeric extract used in the method according to this embodiment may be in the above-mentioned form of the composition of the present invention.

[0061] The subject in the method according to this embodiment is typically a human, but may be a mammal other than a human.

[0062] In the method according to this embodiment, the administration route is preferably oral or nasal administration, and particularly preferably oral administration.

[0063] In the method according to this embodiment, the dose of the turmeric extract administered to a subject is not particularly limited as long as it is an effective amount for treating, preventing, or ameliorating a disease or disorder associated with insulin resistance. For example, when the subject is an adult, the turmeric extract is preferably administered, based on the components contained therein, so that the total amount of turmeronol A and turmeronol B per adult is 100 μg or more, and / or turmeronol A is 17 μg or more, preferably 28 μg or more, more preferably 43 μg or more, even more preferably 57 μg or more, most preferably 80 μg or more, for example 100 μg, and / or turmeronol B is 5 μg or more, preferably 8 μg or more, more preferably 12 μg or more, even more preferably 16 μg or more, most preferably 20 μg or more, for example 100 μg, and / or bisaclone is 80 μg or more, preferably 133 μg or more, more preferably 200 μg or more, even more preferably 267 μg or more, most preferably 360 μg or more, for example 400 μg. EXAMPLES

[0064] [Reference example] (1) Method for producing turmeric extract Turmeric extracts were prepared by water extraction and supercritical gas extraction. In the water extraction method, the turmeric rhizome parts were extracted with water (including hot water) and the resulting extract was dried under reduced pressure to remove moisture. In the supercritical gas extraction method, the turmeric rhizome parts were contacted with supercritical carbon dioxide and the extracted components were mixed with vegetable oil. In the following examples, the turmeric extracts prepared by each extraction method were mixed at a weight ratio of 8:1 and used.

[0065] (2) Test foods containing turmeric extract The test food is a soft capsule containing 100 μg of turmeronol A (TA), 100 μg of turmeronol B (TB), and 400 μg of bisacrol per two capsules of the turmeric extract prepared in Example 1. The test food is a soft capsule made from vegetable oil, gelatin, glycerin, emulsifier (derived from soybeans), and beeswax as raw materials. The placebo food was a soft capsule of the above test food that did not contain turmeric extract.

[0066] (3) Amount of Turmeronol A, Turmeronol B and Bisacron in Turmeric Extract The amounts of turmeronol A, turmeronol B and bisacron in the turmeric extract were measured by HPLC as follows: The amount of turmeronol A per gram of turmeric extract was confirmed to be 724 μg, the amount of turmeronol B per gram of turmeric extract was 578 μg, and the amount of bisacron per gram of turmeric extract was 2182 μg.

[0067] Turmeronol A Analysis Turmeric extract was weighed, water and ethyl acetate were added, and the mixture was centrifuged to obtain a supernatant. The ethyl acetate was removed by vacuum distillation, and the mixture was dissolved in acetonitrile. The obtained liquid was used as a measurement sample and subjected to HPLC. HPLC was performed under the following conditions. Column: L-column ODS 5μm (4.6 mm φ×250 mm) Mobile phase: 48% acetonitrile (20 min) ⇒ 100% acetonitrile (5 min) ⇒ 48% acetonitrile (10 min) Flow rate: 1.0mL / min Column temperature: 40℃ Detection wavelength: 238 nm

[0068] Analysis of Turmeronol B Turmeric extract was weighed, water and ethyl acetate were added, and the mixture was centrifuged to obtain a supernatant. The ethyl acetate was removed by vacuum distillation, and the mixture was dissolved in acetonitrile. The obtained liquid was used as a measurement sample and subjected to HPLC. HPLC was performed under the following conditions. Column: COSMOSIL 5PYE Packed Column (4.6 mm φ × 150 mm) Mobile phase: 57% methanol (30 min) ⇒ 100% methanol (5 min) ⇒ 57% methanol (15 min) Flow rate: 1.0mL / min Column temperature: 40℃ Detection wavelength: 238 nm

[0069] Analysis of Bisacron Turmeric extract was weighed, water and ethyl acetate were added, and the mixture was centrifuged to obtain a supernatant. The ethyl acetate was removed by vacuum distillation, and the mixture was dissolved in acetonitrile. The obtained liquid was used as a measurement sample and subjected to HPLC. HPLC was performed under the following conditions. Column: Wakopak Navi C18-5 (4.6 mm φ × 250 mm) Mobile phase: 30% acetonitrile (20 min) ⇒ 20% acetonitrile (5 min) ⇒ 80% acetonitrile (10 min) Flow rate: 0.7mL / min Column temperature: 50℃ Detection wavelength: 240nm

[0070] [Example 1] Oral glucose tolerance test (OGTT) Male and female subjects aged 40-69 were randomly assigned to two groups and given either a test food containing turmeric extract or a placebo food not containing the extract, two tablets per day before dinner, under double-blind conditions, for 12 weeks (placebo group: n=42, turmeric extract group: n=39). After 12 weeks of taking the test food, an OGTT was performed and blood glucose and insulin levels were measured.

[0071] The specific method of OGTT is as follows. The subjects fasted from the night before the test, and on the day of the test, blood was drawn immediately before glucose loading (0 minutes). The subjects then ingested 75 g of Trelan G solution (a partial starch hydrolysate solution), and blood was drawn 30, 60, and 120 minutes later, and blood glucose and insulin levels were measured. The results were shown as the change from immediately before glucose loading (0 minutes).

[0072] In addition, the Cederholm index, an index of insulin sensitivity (ISI), was calculated. The Cederholm index was proposed as an index of insulin sensitivity by Cederholm and Wibell (Cederholm, J. and Wibell, L. Diabetes Res Clin Pract. 10(2):167-75, 1990; Radikova, Z. Endocr Regul. 37(3):189-94, 2003). The ISI, which is the Cederholm index, was calculated. Cederholm is calculated according to the following formula. This is an indicator that the higher the value, the higher the insulin sensitivity, and the lower the value, the lower the insulin sensitivity.

[0073]

number

[0074] The changes in blood glucose (mg / dL) and insulin (μU / mL) levels from before (0 min) to 30, 60, and 120 min after ingestion of Trelan G solution are shown in Figures 1 and 2. The data shown in Figures 1 and 2 are the mean ± SEM of the turmeric extract intake group (n=39) or the placebo group (n=42), and ** indicates p < 0.01 vs. placebo group. As can be seen from Figures 1 and 2, when Trelan G solution was ingested, both blood glucose and insulin levels rose rapidly from 0 min (baseline).

[0075] As shown in Figure 1, the blood glucose levels of the turmeric extract group were significantly lower at both 60 and 120 minutes after the glucose load compared to the placebo group. This suggests that ingesting turmeric extract inhibits the rise in blood glucose levels after a meal (or promotes the disappearance of blood glucose levels (=promotes the return to baseline)). In addition, as shown in Figure 2, the insulin levels of the turmeric extract group were significantly lower at 120 minutes compared to the placebo group. After a meal, insulin is secreted in large amounts from the pancreas to lower blood glucose levels, but as blood glucose levels drop, insulin becomes unnecessary and insulin levels return to normal. Therefore, as shown in the results of Figure 2, the blood glucose levels of the turmeric intake group were significantly lower than those of the placebo group, and it is thought that insulin levels also decreased accordingly. Furthermore, as shown in Figure 3, the change from week 0 to week 12 in the Cederholm index (ΔCederholm index), an index of insulin sensitivity, in the turmeric extract group was significantly higher than in the placebo group (p < 0.05), suggesting that turmeric extract improves insulin sensitivity.

[0076] There are various mechanisms for controlling postprandial blood glucose levels, including glucagon and hepatic gluconeogenesis, but because insulin levels rise rapidly, it is characterized by the fact that insulin has a much greater influence than other control factors. Therefore, the improvement in blood glucose levels due to the intake of turmeric extract is likely due to the promotion of insulin secretion or the improvement of insulin resistance. In this study, although an increase in insulin levels (promoting effect on insulin secretion) was not observed in the turmeric extract group, an improvement in blood glucose levels was observed (Figures 1 and 2). Furthermore, improvement in insulin sensitivity was also observed in the turmeric extract group (Figure 3). Therefore, it can be interpreted as an improvement in the effectiveness of insulin, in other words, an improvement in insulin resistance.

[0077] These results demonstrate that intake of turmeric extract improves insulin resistance.

Claims

1. A composition for improving insulin resistance, comprising turmeric extract as an active ingredient.

2. A composition for treating, preventing, or ameliorating a disease or disorder associated with insulin resistance, comprising turmeric extract as an active ingredient.

3. 3. The composition according to claim 1 or 2, wherein the composition consists solely of turmeric extract as an active ingredient.

4. 3. The composition according to claim 1 or 2, wherein the turmeric extract comprises an extract of turmeric with at least one extraction solvent selected from the group consisting of water and hydrophilic organic solvents.

5. 3. The composition according to claim 1 or 2, wherein the turmeric extract comprises a mixture of a turmeric extract obtained by extraction with water and a turmeric extract obtained by extraction with supercritical carbon dioxide.

6. The composition according to claim 1 or 2, wherein the turmeric extract contains at least one of turmeronol A, turmeronol B and bisacron.

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