Method for diluting ethanolic extract of bitter melon leaf and method for making the same oil-soluble

Abstract

To provide a method for diluting, with water, an ethanolic extract of bitter melon (Momordica charantia) leaves, capable of suppressing formation of deposition as much as possible; and to provide a method for making the ethanolic extract of bitter melon leaves oil-soluble.SOLUTION: There is provided a method for diluting an ethanolic extract of bitter melon (Momordica charantia) leaves by adding water to the ethanolic extract of the bitter melon leaves, with a water extract of Nemacystus decipiens added thereto. There is provided also a method for adding an emulsifier and lecithin to the ethanolic extract of bitter melon leaves, with the water extract of Nemacystus decipiens added thereto, and further preparing an emulsion with oil added thereto to make the ethanolic extract of bitter melon leaves oil soluble with the emulsion added in the oil.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a method for diluting and oil-solubilizing an ethanol extract of momordica charantia leaves. [Background technology]

[0002] The number of patients with allergies in Japan has increased dramatically since the period of high economic growth, due to factors such as the Westernization of the diet, which focuses on bread, and house dust.

[0003] In particular, gluten allergies have problems such as the antigen being derived from staple foods such as bread, and the antigenicity of gluten as an antigen cannot be suppressed even by heat treatment, forcing patients to change the ingredients of their staple foods, which leads to restrictive lifestyles.

[0004] Therefore, if we can suppress the onset of allergies through our daily diet, we can reduce the burden.

[0005] The present inventors have previously proposed a gluten allergy suppressant in tablet form comprising a dried extract from momordica charantia leaves (see Patent Document 1).

[0006] According to such a gluten allergy suppressant, it is possible to provide a gluten allergy suppressant using natural products that can suppress the onset of gluten allergy through daily eating habits. [Prior art documents] [Patent documents]

[0007] [Patent Document 1] JP 2020-079212 A Summary of the Invention [Problem to be solved by the invention]

[0008] Incidentally, the gluten allergy suppressant mentioned above is a solid (tablet-shaped) preparation made by drying the extract from momordica charantia leaves. However, if one wishes to increase the variety of dosage forms, such as liquid or jelly, it is simpler to simply use the extract, which is originally in liquid form.

[0009] However, when the original extract of momordica charantia leaves is an ethanol extract, there is a problem in that when it is diluted with water to prepare an aqueous gluten allergy suppressant, or when it is made oil-soluble to prepare an oil-based gluten allergy suppressant, insoluble components precipitate.

[0010] Furthermore, such problems are not limited to the use of an ethanol extract of momordica charantia leaves as an agent for suppressing gluten allergies, but rather significantly limit the scope of use of an ethanol extract of momordica charantia leaves, where various effects of momordica charantia leaves are expected, such as improving dementia.

[0011] The present invention has been made in consideration of the above circumstances, and provides a method for diluting an ethanol extract of momordica charantia leaves with water and a method for dissolving an ethanol extract of momordica charantia leaves in oil, which can minimize the formation of precipitation. [Means for solving the problem]

[0012] In order to solve the above-mentioned problems, the method for diluting an ethanol extract of momordica charantia leaves according to the present invention comprises (1) adding water to the ethanol extract of momordica charantia leaves to which a water extract of mozuku has been added to dilute the ethanol extract by adding water.

[0013] In addition, in the method of the present invention for solubilizing an ethanol extract of momordica charantia leaves in oil, (2) an emulsifier and lecithin are added to the ethanol extract of momordica charantia leaves to which a water extract of mozuku has been added, and oil is further added to prepare an emulsion, which is then added to the oil.

[0014] In addition, in the present invention, (3) a water extract of Mozuku seaweed is used as a precipitation inhibitor for the ethanol extract of momordica charantia leaves. Effect of the Invention

[0015] According to the method for diluting an ethanol extract of momordica charantia leaves of the present invention, the ethanol extract of momordica charantia leaves to which a water extract of mozuku has been added is diluted by adding water, thereby providing a method for diluting an ethanol extract of momordica charantia leaves with water that can minimize the formation of precipitation.

[0016] Furthermore, according to the method for solubilizing an ethanol extract of momordica charantia leaves in oil of the present invention, an emulsifier and lecithin are added to the ethanol extract of momordica charantia leaves to which a water extract of mozuku has been added, and then oil is added to prepare an emulsion, and this emulsion is then added to the oil. Therefore, a method for solubilizing an ethanol extract of momordica charantia leaves in oil can be provided which can minimize the formation of precipitation.

[0017] In addition, since a water extract of Mozuku is used as a precipitation inhibitor for the ethanol extract of momordica charantia leaves, it is possible to minimize the formation of precipitation when the ethanol extract of momordica charantia leaves is diluted with water or when the ethanol extract of momordica charantia leaves is solubilized in oil. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0018] The present invention provides a method for diluting an ethanol extract of momordica charantia leaves, which is an extract of momordica charantia with ethanol and contains an extract from the leaves and stems of momordica charantia as an active ingredient. The main ingredients of this extract are momordicin, charantin and corosolic acid, but other ingredients may also be included.

[0019] Furthermore, leaves of other plants of the genus Momordica (ie, Momordica), such as Momordica longa, Momordica trifolia, and Momordica napus, may be used as a source of the ethanol extract of momordica leaves.

[0020] The leaves and stems of the momordica charantia used as the raw material may be collected from momordica charantia grown with buds or from momordica charantia grown without buds. However, the leaves and stems collected from momordica charantia grown without buds are more preferable because the active ingredient can be concentrated in the leaves and stems of momordica charantia grown without buds.

[0021] The extraction from momordica charantia leaves with ethanol may be performed at room temperature, or with hot ethanol, the temperature of which may be, for example, 30 to 95°C.

[0022] The ethanol used for extraction may contain water, for example when diluted with water, with the allowable water content being greater than 0 w / w% and generally not more than 50 w / w%.

[0023] A feature of the method for diluting the ethanol extract of momordica charantia leaves according to the present invention is that a water extract of mozuku seaweed is added to the ethanol extract of momordica charantia leaves prior to dilution with water.

[0024] The mozuku seaweed used as the raw material for the water extract of mozuku seaweed is preferably Okinawa mozuku seaweed (Cladosiphon okamuranus), a type of brown algae belonging to the family Chondromedaceae in the order Psilopidales.

[0025] The water extract of Mozuku is an extract obtained by washing Mozuku and extracting it with water or hot water either untreated or after a specific treatment, and contains a large amount of fucoidan and fucose. Examples of the specific treatment include acid treatment and base treatment (alkali treatment).

[0026] The amount of the water extract of Mozuku added to the ethanol extract of Momordica charantia leaves is not particularly limited, and may be an amount that can achieve the purpose, i.e., an amount that can suppress the precipitation of insoluble components. It may be appropriately adjusted when used. However, since the present application is a new proposal in this regard, the amount of the water extract of Mozuku added to the ethanol extract of Momordica charantia leaves will be discussed later.

[0027] In this way, the ethanol extract of momordica leaves to which has been added a water extract of mozuku seaweed is able to suppress the precipitation of insoluble components even when diluted with water, making it possible to prepare an aqueous preparation of stable quality with minimal precipitate formation.

[0028] The present invention also provides a method for making an ethanol extract of momordica charantia leaves oil-soluble. When oil is added to an ethanol extract of momordica charantia leaves, precipitation of insoluble components occurs.

[0029] Therefore, in the method of the present invention for solubilizing an ethanol extract of momordica charantia leaves in oil, an emulsifier and lecithin are added to the ethanol extract of momordica charantia leaves to which a water extract of mozuku has been added, and then oil is added to prepare an emulsion, which is then added to the oil.

[0030] Here, the amount of the water extract of Mozuku seaweed added to the ethanol extract of Momordica charantia leaves required for oil solubilization is not particularly limited, and may be an amount that can achieve the purpose, i.e., an amount that can suppress the precipitation of insoluble components. It can be appropriately adjusted when used. However, since the present application also proposes a new point on this point, it will be mentioned later.

[0031] The ethanol extract of momordica leaves to which the aqueous extract of mozuku has been added is then subjected to the addition of an emulsifier and lecithin.

[0032] The emulsifier is not particularly limited as long as it has an HLB value of about 1 to 16, and commercially available emulsifiers such as lecithin-based and fatty acid glyceryl-based emulsifiers can be used.

[0033] The amount of emulsifier added may be any amount that is capable of emulsifying or solubilizing insoluble components, and varies depending on the type of emulsifier used. In general, however, it is added at a concentration of approximately 0.1 to 50 mg / ml to the momordica leaf ethanol extract to which the aqueous extract of mozuku has been added.

[0034] As the lecithin, for example, phosphatidylcholine-rich lecithin or hydrogenated lecithin can be used.

[0035] The amount of lecithin added may be any amount that is capable of emulsifying or solubilizing insoluble components, and varies depending on the type of lecithin used. In general, however, lecithin is added at a concentration of approximately 0.1 to 50 mg / ml to the momordica leaf ethanol extract to which the aqueous extract of mozuku has been added.

[0036] Next, oil is added to the ethanol extract of momordica leaf to which the water extract of mozuku, emulsifier, and lecithin have been added, and emulsified.

[0037] The oil for this emulsification can be appropriately selected depending on the product using the ethanol extract of momordica charantia leaves, for example, if the product is food, it is desirable to select an oil that is suitable for cooking, and if the product is medicine, it is desirable to select an oil that can be used at a pharmaceutical level. To give a specific example, for example, olive oil or MCT (medium chain fatty acid) oil can be suitably used for food.

[0038] The amount of oil used for emulsification is not particularly limited, but may be an amount equal to or greater than the amount that can emulsify the total amount of the momuzu water extract, the emulsifier, and the momuzu leaf ethanol extract to which has been added lecithin.

[0039] The thus prepared emulsion is added to the dissolving oil, so that the ethanol extract of momordica charantia leaves is oil-soluble. The emulsifying oil and the dissolving oil may be the same or different. The emulsifying oil and the dissolving oil may be added in separate steps, or may be added in a single step to perform both emulsification and dissolving.

[0040] Furthermore, when considering the method for diluting and the method for oil-solubilizing the ethanol extract of momordica charantia leaves according to this embodiment from a different angle, it can also be said that the present application provides a method for using a water extract of mozuku seaweed as a suspending agent for an ethanol extract of momordica charantia leaves.

[0041] Incidentally, as described above, the amount of the water extract of Mozuku seaweed added to the ethanol extract of momordica charantia leaves, which is necessary for dilution with water or oil solubilization, is not particularly limited as long as it is an amount that can suppress precipitation of insoluble components.

[0042] However, the amount of water extract of Mozuku required to obtain the effect of inhibiting precipitation may need to be adjusted depending on the concentration of the ethanol extract of momordica charantia leaves and the concentration of the water extract of Mozuku, making the determination of the amount somewhat complicated.

[0043] In addition, the specific mechanism of precipitation inhibition by the aqueous extract of Mozuku is awaited for further research results, and since the precipitation-inhibiting component contained in the aqueous extract of Mozuku is unknown at present, the content of the precipitation-inhibiting component cannot be used as an indicator. Similarly, the mechanism of why precipitation occurs when the ethanol extract of Momordica charantia leaves is diluted or dissolved in oil is also unknown at present, so it is difficult to determine what should be used as a guide to determine the amount of aqueous extract of Mozuku to be added.

[0044] In this respect, in the present invention, the amount of the aqueous extract of Mozuku added to the ethanol extract of momordicine leaves is determined based on the momordicine derived from the ethanol extract of momordicine leaves and the fucose derived from the aqueous extract of Mozuku, and the ethanol extract of momordicine leaves and the aqueous extract of Mozuku are mixed so that momordicine and fucose are in a predetermined ratio. It is possible to use the total momordicine as the target component for momordicine, or to use each individual momordicine as the target. For example, this embodiment is characterized in that the amount of the aqueous extract of Mozuku added to the ethanol extract of momordicine leaves is determined based on the amount of fucose contained in the aqueous extract of Mozuku per 1 mg of momordicine I contained in the ethanol extract of momordicine leaves.

[0045] Although this is merely within the range of current expectation, it will be mentioned for the sake of understanding of the present invention that the present inventors believe that momordicine is not directly involved in the formation of precipitates upon dilution or oil solubilization, and that fucose is not directly involved in the inhibition of the formation of precipitates.

[0046] However, based on the experience gained through the intensive research of the present inventors, it has been found that by using the amount of momordicine and the amount of fucose as indicators and adjusting the amounts of both within the above ranges, the formation of precipitates during dilution or oil solubilization can be effectively suppressed, which is extremely useful in eliminating the complexity involved in adjusting the amounts added.

[0047] As one example, the amount of aqueous extract of Mozuku added to the above-mentioned ethanol extract of momordicin I in the ethanol extract of momordicin I leaves is 0.0001 to 0.005 w / w%, and the fucose concentration in the aqueous extract of Mozuku is 0.01 to 1 w / w%, and a precipitation-inhibiting effect can be expected when the ethanol extract of momordicin I leaves is diluted or oil-solubilized so that the final concentration of the ethanol extract of momordicin I leaves is less than 100 w / w% and is 0.1 w / w% or more.

[0048] The method for diluting and oil-solubilizing the momordica charantia leaf ethanol extract according to this embodiment will be explained in further detail below with reference to experimental examples showing the preparation process of momordica charantia leaf extract, the preparation process of mozuku water extract (hereinafter also referred to as mozuku water extract), and the use of mozuku water extract as a suspending agent for momordica charantia leaf ethanol extract.

[0049] 1. Preparation of ethanol extract from momordica charantia leaves 100g of momordica charantia leaves were placed in a 3L beaker, 2kg of 95vol% ethanol at about 20℃ was added, and the mixture was stirred for about 1 to 4 hours to carry out an extraction operation. Next, the momordica charantia leaves were removed by filtration, and the ethanol was removed to obtain an ethanol extract of momordica charantia leaves. The concentration of momordicin I in the ethanol extract of momordica charantia leaves thus prepared was 0.03mg / g.

[0050] 2. Preparation of Mozuku Water Extract 100g of mozuku (Cladosiphon okamuranus) was placed in a 1L beaker, 500g of water was added, and the mixture was heated at 90°C for 3 hours while stirring well. After that, the mozuku was removed, and the resulting solution was used as a mozuku water extract. The fucose concentration of the mozuku water extract thus obtained was 5mg / g.

[0051] [3. Water solubility test] Next, a water-solubilization test was carried out using the above-mentioned ethanol extract of momordica charantia leaves. Specifically, the presence or absence of a water extract of mozuku seaweed was examined to see how the precipitation of insoluble components when the ethanol extract of momordica charantia leaves was diluted with water.

[0052] In the test, the presence or absence of insoluble components was examined by visually comparing two samples: Comparative sample 1, which was solubilized without adding a water extract of Mozuku, and Confirmation sample 2, which was solubilized by adding a water extract of Mozuku.

[0053] Comparative sample 1 was prepared by dispensing 5 g (0.15 mg as momordicin I) of an ethanol extract of momordica charantia leaves into a 200 ml beaker, and adding 95 g of water to prepare a 5 w / w % aqueous solution.

[0054] For confirmation sample 2, 5 g (0.15 mg as momordicin I) of ethanol extract of momordica leaf was dispensed into a 200 ml beaker, 0.015 g (0.075 mg as fucose) of water extract of mozuku seaweed was added, and then 94.985 g of water was added to make a 5% aqueous solution. The results of the water-solubilization test are shown in Table 1. [Table 1]

[0055] As can be seen from the results of Comparative Sample 1 shown in Sample No. 1 in Table 1, when an ethanol extract of momordica charantia leaves is added to an aqueous solution, insoluble components precipitate. However, as can be seen from the results of Confirmation Sample 2 of Sample No. 2, when a water-extracted water extract of Mozuku seaweed was added to an ethanol extract of momordica charantia leaves before adding water, no precipitation of insoluble components was confirmed.

[0056] It is believed that the water extract of Mozuku contributed to the solubilization of insoluble components, but up until now it has not been thought that water extract of Mozuku in general has solubilizing properties.

[0057] Furthermore, additional experiments have revealed that the ideal ratio of mozuku water extract to ethanol extract of momordica charantia leaves is in the range of 0.01 to 10%, as less than this amount is ineffective, while more than this amount causes insoluble components to precipitate.

[0058] From these findings, it was demonstrated that the method of diluting the ethanol extract of momordica charantia leaves in this embodiment is a method of diluting the ethanol extract of momordica charantia leaves with water, which can minimize the formation of precipitates due to insoluble components.

[0059] [4. Oil solubility test] Next, an oil-solubilization test was carried out using the above-mentioned ethanol extract of momordica charantia leaves. Specifically, the authors investigated how the precipitation of insoluble components when the ethanol extract of momordica charantia leaves was dissolved in oil when an emulsifier, lecithin, or a water extract of Mozuku seaweed was added to the extract.

[0060] The tests involved visually comparing three comparison samples - comparison sample 3, in which an attempt was made to be oil-soluble without the addition of any emulsifier, lecithin, or mozuku water extract; comparison sample 4, in which an attempt was made to be oil-soluble with the addition of an emulsifier but without the addition of lecithin or mozuku water extract; and comparison sample 5, in which an attempt was made to be oil-soluble with the addition of an emulsifier and lecithin but without the addition of mozuku water extract - with confirmation sample 6, in which an emulsifier, lecithin, and mozuku water extract were added, to examine the presence or absence of insoluble components.

[0061] Comparative sample 3 was prepared by dispensing 5 g (0.15 mg as momordicin I) of an ethanol extract of momordica charantia leaves into a 200 ml beaker, and adding 95 g of olive oil to prepare a 5 w / w % solution.

[0062] Comparative sample 4 was prepared by dispensing 5 g (0.15 mg as momordicin I) of ethanol extract of momordica charantia leaves into a 200 ml beaker, adding 1 g of emulsifier, and then adding 94 g of olive oil to make a 5 w / w% solution.

[0063] Comparative sample 5 was prepared by dispensing 5 g (0.15 mg as momordicin I) of ethanol extract of momordica charantia leaves into a 200 ml beaker, adding 1 g of emulsifier and 0.2 g of lecithin, and adding 93.8 g of olive oil to make a 5 w / w% solution.

[0064] Confirmation sample 6 was prepared by dispensing 5g (0.15mg as momordicin I) of ethanol extract of momordica leaf into a 200ml beaker, adding 0.015g (0.075mg as fucose) of water extract of mozuku seaweed and mixing, then adding 1g of emulsifier and mixing, then adding 0.2g of lecithin and mixing, and further adding 93.785g of olive oil and thoroughly emulsifying and stirring to obtain a 5w / w% solution. Table 2 shows the results of the oil-solubilization test. [Table 2]

[0065] As can be seen from the results of Comparative Sample 3 shown as Sample No. 3 in Table 2, when momordica charantia leaf extract or momordica charantia leaf extract is added to oil, precipitation of insoluble components occurs.

[0066] Therefore, first, an attempt was made to add an emulsifier to the momordica leaf extract to make it oil-soluble, as in Comparative Sample 4 shown in Sample No. 4, but this resulted in the production of insoluble components.

[0067] Next, as in Comparative Sample 5 shown in Sample No. 5, an attempt was made to dissolve the momordica leaf extract in oil by adding an emulsifier and lecithin, but this also resulted in the production of insoluble components.

[0068] Next, when a water extract of Mozuku was further added as in Confirmation Sample 6 shown in Sample No. 6, no precipitation of insoluble components was confirmed.

[0069] From these findings, it was demonstrated that the method for solubilizing an ethanol extract of momordica charantia leaves in this embodiment is a method for solubilizing an ethanol extract of momordica charantia leaves in oil that can minimize the formation of precipitate due to insoluble components.

[0070] [5. Examination of the precipitation inhibition effect of seaweed extracts other than Mozuku] As described above, the present application is characterized in that an aqueous extract of Mozuku is used as a precipitation inhibitor for an ethanol extract of momordica charantia leaves. Therefore, in this section, we investigated whether seaweed extracts other than Mozuku can exhibit a precipitation prevention (precipitation suppression) effect for an ethanol extract of momordica charantia leaves.

[0071] Seaweed is classified into brown algae, red algae, and green algae. In this study, three types of seaweed water extracts were prepared using three types of seaweed: brown algae, Hibamata, red algae, and green algae. Confirmation tests for the anti-precipitation effect of momordica charantia leaf ethanol extract were conducted as follows.

[0072] (5-1) Sample preparation The preparation of the ethanol extract of momordica charantia leaves was the same as in [1. Preparation of ethanol extract of momordica charantia leaves] described above. The preparation of the seaweed water extract was carried out by first putting 100g of seaweed into a 1L beaker, adding 500g of water, and heating at 90°C for 3 hours while stirring well, then removing the seaweed, and the resulting solution was used as the seaweed water extract. Three types of seaweed water extracts were prepared: a brown algae water extract, a red algae water extract, and a green algae water extract, a Fucus vesiculosus water extract.

[0073] (5-2) Water solubility test Next, a water-solubilization test was conducted using the above-mentioned ethanol extract of momordica charantia leaves to confirm whether seaweed extracts of Fucus vesiculosus, Fukuronori, and Aonori have the same effect as the water extract of Mozuku seaweed. Specifically, the authors investigated how the precipitation of insoluble components when the ethanol extract of momordica charantia leaves was diluted with water, depending on whether or not each seaweed water extract was added.

[0074] The test consisted of visually comparing the presence or absence of insoluble components in two samples: comparison sample 1, which was solubilized without adding seaweed water extract, and confirmation samples 7, 8, and 9, which were solubilized by adding seaweed water extract.

[0075] Comparative sample 1 was prepared by dispensing 5 g (0.15 mg as momordicin I) of an ethanol extract of momordica charantia leaves into a 200 ml beaker, and adding 95 g of water to prepare a 5 w / w % aqueous solution.

[0076] For confirmation samples 7, 8, and 9, 5 g (0.15 mg as momordicin I) of ethanol extract of momordicine leaves was dispensed into a 200 ml beaker, 0.015 g of seaweed water extract was added, and then 94.985 g of water was added to make a 5% aqueous solution. The results of the water-solubilization test are shown in Table 3. [Table 3]

[0077] As can be seen from the results of Comparative Sample 1 shown as Sample No. 1, when water is added to the ethanol extract of momordica charantia leaves, insoluble components are precipitated. Similarly, the results of Confirmatory Samples 7, 8, and 9 also confirmed the precipitation of insoluble components.

[0078] These findings indicate that the precipitation-preventing effect of the ethanol extract of momordica charantia leaves is not an effect typically exhibited by aqueous extracts of common brown, red, or green algae, but is an effect unique to the aqueous extract of Mozuku seaweed.

[0079] (5-3) Oil solubility test Next, using the ethanol extract of the above-mentioned momordica charantia leaves, it was confirmed whether the seaweed extracts of Hibamata, Fukuronori, and Aonori would produce the same effect as the aqueous extract of Mozuku seaweed when solubilized in oil.

[0080] Specifically, an emulsifier and lecithin were added to an ethanol extract of momordica charantia leaves, and then the researchers investigated how the precipitation of insoluble components when oil was added and dissolved changed depending on whether or not each seaweed water extract was added.

[0081] The test consisted of visually comparing comparison sample 5, which was made oil-soluble by adding an emulsifier and lecithin but not each seaweed water extract, with confirmation samples 10, 11, and 12, which contained emulsifiers, lecithin, and each seaweed water extract, to examine whether or not there were any insoluble components.

[0082] For the confirmation samples 10, 11, and 12, 5 g (0.15 mg as momordicin I) of ethanol extract of momordicine leaves was dispensed into a 200 ml beaker, 0.015 g of seaweed water extract was added and mixed, 1 g of emulsifier was added and mixed, 0.2 g of lecithin was added and mixed, and 93.785 g of olive oil was added and thoroughly emulsified and stirred to obtain a 5 w / w% solution. Table 4 shows the results of the oil solubilization test. [Table 4]

[0083] As can be seen from the results of Comparative Sample 5 shown as Sample No. 5, when oil is added to the ethanol extract of momordica charantia leaves, insoluble components precipitate, even if emulsifiers and lecithin are present. Similarly, the results of Confirmatory Samples 10, 11, and 12, also confirmed the precipitation of insoluble components.

[0084] These findings indicate that the precipitation-preventing effect of the ethanol extract of momordica charantia leaves is not an effect typically exhibited by aqueous extracts of common brown, red, or green algae, but is an effect unique to the aqueous extract of Mozuku seaweed.

[0085] [6. Confirmation test of whether momordicine is involved in the precipitation of insoluble components] With regard to the method for diluting an ethanol extract of momordica charantia leaves, the method for oil-solubilizing an ethanol extract of momordica charantia leaves, and the method for using an aqueous extract of mozuku as a suspending agent for an ethanol extract of momordica charantia leaves according to the present embodiment, the amount of aqueous extract of mozuku added to an ethanol extract of momordica charantia leaves can be, as described above, an amount such that 0.01 to 1 mg of fucose is contained in the aqueous extract of mozuku per 1 mg of momordica I contained in the ethanol extract of momordica charantia leaves.

[0086] As mentioned above, the present inventors believe that momordicine is not directly involved in the formation of precipitates upon dilution or oil solubilization, and that fucose is not directly involved in the inhibition of precipitate formation.

[0087] In this section, therefore, we investigated whether momordicine is directly involved in the formation of precipitates during dilution or oil-solubilization by replacing the ethanol extract of momordicine with pure momordicine and diluting it with water or oil-solubilization.

[0088] In order to investigate the involvement of momordicine, it would be ideal to add an ethanol extract of momordicine from which only momordicine has been removed, instead of the usual ethanol extract of momordicine, and demonstrate that the precipitation of insoluble components is suppressed by the ethanol extract of momordicine even in the absence of momordicine. However, it is extremely difficult to remove only momordicine from the ethanol extract of momordicine.

[0089] Therefore, in this section, we used pure momordicine and made the final concentration of momordicine when diluted with water or solubilized in oil twice the final concentration of momordicine derived from an ethanol extract of momordicine leaves to confirm whether the amount of insoluble components that precipitate when no aqueous extract of Mozuku is used changes, for example, whether it increases by about two-fold, and to examine whether momordicine is directly involved in the formation of precipitates.

[0090] (6-1) Water solubility test Comparative sample 1 was prepared by dispensing 5 g (0.15 mg as momordicin I) of ethanol extract of momordicin leaves into a 200 ml beaker and adding 95 g of water to prepare a 5 w / w% aqueous solution. The final concentration of momordicin I in comparative sample 1 was 0.15 mg / 100 g.

[0091] Comparative sample 13 was prepared by adding 0.15 mg of momordicin I (98% or higher purity, ChemFaces) to 5 g (0.15 mg as momordicin I) of ethanol extract of bitter melon leaves, dissolving the solution, dispensing the solution in a 200 ml beaker, and adding 95 g of water to obtain a 5 w / w% aqueous solution. The final concentration of momordicin I in comparative sample 13 was approximately 0.30 mg / 100 g. Table 5 shows the results of the water-solubilization test. [Table 5]

[0092] As shown in Table 5, precipitation of insoluble components was detected in both Comparative Sample 1 and Comparative Sample 13. Furthermore, the amount of precipitation in Comparative Sample 13 was almost the same as the amount of precipitation in Comparative Sample 1 when compared visually.

[0093] From these findings, it was suggested that momordicine is not directly involved in precipitation in the dilution method of the ethanol extract of momordica charantia leaves according to the present embodiment, or in the use of the aqueous extract of mozuku as a precipitation inhibitor for the ethanol extract of momordica charantia leaves. It is very interesting that, even though momordicine is not directly involved in precipitation, the amount of momordicine can be used as a guide for determining the amount of the aqueous extract of mozuku to be added to the ethanol extract of momordica charantia leaves.

[0094] (6-2) Oil solubility test Comparative sample 5 was prepared by dispensing 5 g (0.15 mg as momordicin I) of ethanol extract of momordicin leaves into a 200 ml beaker, adding 1 g of emulsifier and mixing, then adding 0.2 g of lecithin and mixing, and further adding 93.8 g of olive oil, and thoroughly emulsifying and stirring to obtain a 5 w / w% solution. The final concentration of momordicin I in comparative sample 5 was 0.15 mg / 100 g.

[0095] The confirmation sample 14 was prepared by dissolving 0.15 mg of momordicin I (98% or higher purity, ChemFaces) in 5 g (0.15 mg as momordicin I) of ethanol extract of bitter melon leaves, distributing the solution in a 200 ml beaker, adding 1 g of emulsifier and mixing, then adding 0.2 g of lecithin and mixing, and further adding 93.8 g of olive oil, and thoroughly emulsifying and stirring to obtain a 5 w / w% solution. The final concentration of momordicin I in the comparison sample 14 was approximately 0.30 mg / 100 g. Table 6 shows the results of the oil-solubilization test. [Table 6]

[0096] As shown in Table 6, precipitation of insoluble components was detected in both Comparative Sample 5 and Comparative Sample 14. Furthermore, the amount of precipitation in Comparative Sample 14 was almost the same as the amount of precipitation in Comparative Sample 5 when compared visually.

[0097] These findings suggest that momordicine is not directly involved in precipitation when using the method for solubilizing the ethanol extract of momordica charantia leaves in oil according to this embodiment or when using the aqueous extract of mozuku as a precipitation inhibitor for the ethanol extract of momordica charantia leaves.

[0098] [7. Confirmation test of whether fucose is involved in the precipitation of insoluble components] As in the above [6. Confirmation test of whether momordicin is involved in the precipitation of insoluble components], in this section, we replaced the water extract of Mozuku with pure fucose and diluted it with water or made it oil-soluble to examine whether fucose is directly involved in the formation of precipitation during dilution or oil-solubilization.

[0099] In order to investigate the involvement of fucose, it would be ideal to add an aqueous extract of Mozuku from which only the fucose has been removed, instead of the usual aqueous extract of Mozuku, and demonstrate that the aqueous extract of Mozuku inhibits the precipitation of insoluble components even in the absence of fucose; however, it is extremely difficult to remove only the fucose from the aqueous extract of Mozuku.

[0100] In this section, we therefore used pure fucose to confirm whether the precipitation of insoluble components is suppressed by an aqueous solution with the same fucose concentration as that of the aqueous extract of Mozuku, and to examine whether fucose is directly involved in the formation of precipitation.

[0101] (7-1) Water solubility test Comparative sample 1 was prepared by dispensing 5 g (0.15 mg as momordicin I) of ethanol extract of momordicin leaves into a 200 ml beaker and adding 95 g of water to prepare a 5 w / w% aqueous solution. The final concentration of momordicin I in comparative sample 1 was 0.15 mg / 100 g, and the final concentration of fucose was 0 mg / 100 g.

[0102] Comparative sample 15 was prepared by dispensing 5 g (0.15 mg as momordicin I) of ethanol extract of momordicin leaves into a 200 ml beaker, and adding 95 g of water containing 0.075 mg of fucose (purity 97.0% or higher, manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) to prepare a 5 w / w% aqueous solution. The final concentration of momordicin I in comparative sample 15 was approximately 0.15 mg / 100 g, and the final concentration of fucose was 0.075 mg / 100 g. Table 7 shows the results of the water-solubilization test. [Table 7]

[0103] As shown in Table 7, precipitation of insoluble components was detected in both Comparative Sample 1 and Comparative Sample 15. This suggests that fucose is not directly involved in the inhibition of precipitation when using the dilution method of the ethanol extract of momordica charantia leaves according to the present embodiment or the aqueous extract of mozuku as a precipitation inhibitor for the ethanol extract of momordica charantia leaves. It is very interesting that, even though fucose is not directly involved in the inhibition of precipitation, the amount of fucose can be used as a guide for determining the amount of the aqueous extract of mozuku to be added to the ethanol extract of momordica charantia leaves.

[0104] (7-2) Oil solubility test Comparative sample 5 was prepared by dispensing 5 g (0.15 mg as momordicin I) of ethanol extract of momordicin leaves into a 200 ml beaker, adding 1 g of emulsifier and mixing, then adding 0.2 g of lecithin and mixing, and further adding 93.8 g of olive oil, and thoroughly emulsifying and stirring to obtain a 5 w / w% solution. The final concentration of momordicin I in comparative sample 5 was 0.15 mg / 100 g, and the final concentration of fucose was 0 mg / 100 g.

[0105] The confirmation sample 16 was prepared by dispensing 5 g (0.15 mg as momordicin I) of ethanol extract of bitter melon leaves into a 200 ml beaker, adding 0.015 g of fucose water prepared by adding 0.075 mg of fucose to water and mixing, then adding 1 g of emulsifier and mixing, then adding 0.2 g of lecithin and mixing, and further adding 93.785 g of olive oil and thoroughly emulsifying and stirring to obtain a 5 w / w% solution. The final concentration of momordicin I in the comparison sample 16 was 0.15 mg / 100 g, and the final concentration of fucose was 0.075 mg / 100 g. Table 8 shows the results of the oil-solubilization test. [Table 8]

[0106] As shown in Table 8, precipitation of insoluble components was detected in both Comparative Sample 5 and Comparative Sample 16. This suggests that fucose is not directly involved in suppressing precipitation when using the method for oil-solubilizing an ethanol extract of momordica charantia leaves according to this embodiment or when using an aqueous extract of mozuku seaweed as a precipitation inhibitor for an ethanol extract of momordica charantia leaves.

[0107] As described above, according to the method for diluting an ethanol extract of momordica charantia leaves of this embodiment, the ethanol extract of momordica charantia leaves to which a water extract of mozuku has been added is diluted by adding water, thereby providing a method for diluting an ethanol extract of momordica charantia leaves with water that can minimize the formation of precipitation.

[0108] Furthermore, according to the method for solubilizing an ethanol extract of momordica charantia leaves in oil according to this embodiment, an emulsifier and lecithin are added to the ethanol extract of momordica charantia leaves to which a water extract of mozuku has been added, and then oil is added to prepare an emulsion, and this emulsion is then added to the oil. Therefore, a method for solubilizing an ethanol extract of momordica charantia leaves in oil can be provided that can minimize the formation of precipitation.

[0109] Finally, the above-mentioned embodiments are merely examples of the present invention, and the present invention is not limited to the above-mentioned embodiments. Therefore, even if the above-mentioned embodiments are different, various modifications can be made depending on the design, etc., as long as they do not deviate from the technical idea of ​​the present invention.

Claims

1. A method for diluting an ethanol extract of momordica charantia leaves by adding water to the ethanol extract of momordica charantia leaves to which a water extract of mozuku seaweed has been added.

2. A method for dissolving an ethanol extract of momordica charantia leaves in oil, comprising adding an emulsifier and lecithin to an ethanol extract of momordica charantia leaves to which a water extract of mozuku seaweed has been added, further adding oil to prepare an emulsion, and then adding the emulsion to the oil.

3. Use of an aqueous extract of Mozuku seaweed as a suspending agent for an ethanol extract of momordica charantia leaves.

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