Cell extraction device and cell extraction method

Abstract

To make it possible to efficiently separate and extract vascular cells from a tissue mass only without damaging the vascular cells, and easily secure the number of vascular cells.SOLUTION: A cell dispersion apparatus capable of selectively extracting vascular cells 7 from a tissue mass 5 comprises: a net-like mounting stage 12 on which the tissue mass 5 is mounted; an ejection port part 42 of the nozzle head 41 which ejects high-pressure UFB jet water running water 43 to the tissue mass 5 mounted on the mounting stage 12, and separates the vascular cells 7 from the tissue mass 5; a high-pressure pump part 22 which generates the high-pressure UFB jet water running water 43 ejected from the ejection port part 42; and an ultrahigh pressure pump part 23 which ejects UFB jet water running water 44 of higher pressure to a blood vessel 6 being a residue of the tissue mass 5, to which the UFB jet water running water 43 was ejected, by the ejection port part 42 of the nozzle head 41.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a cell extraction device and a cell extraction method capable of selectively extracting vascular cells from within a tissue mass. [Background technology]

[0002] The present inventors have previously discovered that vascular endothelial stem cells, which exhibit stemness, such as long-term vascular contribution as blood vessels for transplanted organs, exist in existing blood vessels in various organs in the body. Vascular endothelial stem cells are also defined as CD31 (pan vascular endothelial cell marker) positive, CD45 (pan blood cell marker) negative, CD200 positive, CD157 positive, etc.

[0003] By using these vascular endothelial stem cells, it has been found that, for example, it is possible to improve the symptoms of ischemic diseases in the long term and to reconstruct the sinusoidal blood vessels in the liver, and it has also been shown that hemophilia can be completely cured by secreting coagulation factor VIII, a characteristic of sinusoidal vascular endothelial cells.

[0004] It has also been found that molecules genetically introduced into vascular endothelial stem cells can be secreted over the long term within the body, and this has led to the proposal of a new method of cell therapy that will function as a new in-body drug manufacturing factory for the treatment of cancer, metabolic diseases, and other conditions.

[0005] Furthermore, they have proposed a method for reprogramming human umbilical vein endothelial cells to a vascular endothelial stem cell-like phenotype using an extract from liver tissue of mice immediately after birth. [Prior art documents] [Non-patent literature]

[0006] [Non-Patent Document 1] CD157 Marks Tissue-Resident Endothelial Stem Cells with Homeostatic and Regenerative Properties. Wakabayashi T, Naito H, Suehiro JI, Lin Y, Kawaji H, Iba T, Kouno T, Ishikawa-Kato S, Furuno M, Takara K, Muramatsu F, Weizhen J, Kidoya H, Ishihara K, Hayashizaki Y, Nishida K, Yoder MC, Takakura N. Cell Stem Cell. 2018 Mar 1;22(3):384-397 [Patent documents]

[0007] [Patent Document 1] International Publication No. 2019 / 098264 [Patent Document 2] International Publication No. 2021 / 070874 Summary of the Invention [Problem to be solved by the invention]

[0008] However, in order to collect vascular endothelial cells, a complex process is required in which the tissue mass is mechanically and enzymatically treated to disperse the tissue cells, and then the vascular endothelial cells are extracted from the various tissue cells contained therein using cell surface markers.

[0009] In other words, if there were a means to easily collect only vascular tissue from an organ when performing tissue culture or cell culture, it would be possible to isolate (separate) only vascular endothelial cells without damaging vascular cells, and it would also be easier to ensure the number of vascular endothelial cells to be cultured.

[0010] The present invention has been made in consideration of the above, and an object of the present invention is to provide a cell extraction device and a cell extraction method that can efficiently separate and extract only vascular cells from a tissue mass without damaging the vascular cells. [Means for solving the problem]

[0011] In order to achieve the above-mentioned object, an aspect of the present invention is a cell extraction device capable of selectively extracting cells from a tissue mass, characterized in that it comprises a mesh-like mounting section on which the tissue mass is placed, a nozzle section which sprays jet water onto the tissue mass placed on the mounting section to separate the cells from the tissue mass, a generation section which generates the jet water to be sprayed from the nozzle section, and a high-pressure jet water spraying section which sprays higher pressure jet water generated in the generation section from the nozzle section onto the remains of the tissue mass onto which the jet water has been sprayed.

[0012] Another aspect of the present invention is a cell extraction method capable of selectively extracting cells from a tissue mass, comprising the steps of: placing the tissue mass on a mesh-like mounting section; generating jet water by a generation section to be sprayed at the tissue mass; spraying the jet water generated in the generation section from a nozzle section toward the tissue mass placed on the mounting section; and causing a high-pressure jet water spraying section to spray, from the nozzle section, the higher pressure jet water generated in the generation section toward the remains of the tissue mass onto which the jet water has been sprayed, wherein the cells are separated from the tissue mass by switching the pressure of the jet water sprayed from the nozzle section. Effect of the Invention

[0013] According to the present invention, it is possible to provide a cell extraction device and a cell extraction method that can efficiently separate and extract only vascular cells from a tissue mass without damaging the vascular cells. [Brief description of the drawings]

[0014] [Figure 1] 1 is a schematic diagram showing a configuration example of a cell extraction device according to an embodiment of the present invention. [Diagram 2] FIG. 2 is a schematic diagram showing a configuration example of a tissue crushing unit of the cell extraction device according to one embodiment. [Diagram 3] FIG. 2 shows an example of the configuration of a nozzle part of a cell extraction device according to one embodiment, where FIG. 2(a) is a side view and FIG. 2(b) is a front view. [Figure 4] FIG. 4 is a schematic diagram of a UFB jet water stream ejected from the nozzle portion shown in FIG. 3. [Diagram 5] The figures are shown to explain an example of the operation of the tissue crushing unit, with FIG. 1(a) showing the operation during dispersion (separation) and FIG. 1(b) showing the operation during recovery (extraction). [Figure 6] FIG. 1(a) is a micrograph showing an example of a tissue mass, and FIG. 1(b) is a micrograph showing an example of extracted vascular cells. [Figure 7] 13 is a micrograph showing the results of extraction processing of liver blood vessels using a mouse as an example. [Figure 8] FIG. 13 is a schematic diagram showing a configuration example of a cell extraction device according to another embodiment of the present invention. [Figure 9] FIG. 13 is a schematic diagram showing a configuration example of a tissue crushing unit of a cell extraction device according to another embodiment. [Figure 10] The figures are shown to explain an example of the operation of the tissue crushing unit, with Figure (a) showing the operation during separation, and Figures (b) and (c) showing the operation during extraction. [Figure 11] FIG. 13 is a schematic configuration diagram of a tissue crushing unit of a cell extraction apparatus according to still another embodiment of the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0015] Hereinafter, a cell extraction device and a cell extraction method according to an embodiment of the present invention will be described with reference to the drawings. Note that in the embodiments, the drawings are merely schematic illustrations of the invention and may differ from the actual ones.

[0016] One embodiment Fig. 1 is a side view showing a schematic configuration of a cell extraction device according to one embodiment of the present invention. Here, a cell dispersion device for selectively extracting (collecting) vascular cells from, for example, a fat tissue mass of a living body for the human body, or for selectively extracting vascular cells from, for example, a tissue mass of a mouse's liver or kidney for animal experiments will be described as an example.

[0017] The cell dispersion device of this embodiment, as shown in FIG. 1, for example, comprises a tissue grinding unit 1, a solution unit 2, and piping 3 connecting a high-pressure pump section 22 or an ultra-high-pressure pump section 23 in the solution unit 2 to a nozzle component section 40 in the tissue grinding unit 1.

[0018] The tissue crushing unit 1 is a crusher for, for example, decomposing a tissue mass 5 cut out from a living body to be crushed, using UFB (ultra-fine bubble) jet water 43 as jet water, and extracting crushed vascular cells 7 from existing blood vessels 6. The details of the tissue crushing unit 1 will be described later.

[0019] The solution unit 2 includes a water tank section 21 for storing buffer liquid 20 such as saline or PBS (phosphate buffered saline) as a buffer solution for generating the UFB jet water flow 43, a high-pressure pump section 22, and an ultra-high-pressure pump section 23.

[0020] The water bath section 21 has a temperature control function for maintaining the temperature of the buffer solution 20 stored therein at, for example, about 4 to 37° C. In order to reduce damage to cells, the temperature of the buffer solution 20 is preferably about 4° C.

[0021] The high-pressure pump unit 22 is a generating unit for generating a high-pressure jet water flow from the buffer liquid 20 stored in the water tank unit 21. That is, the high-pressure pump unit 22 is capable of generating a high-pressure jet water flow with a water pressure of 100 PSI or more.

[0022] Taking into consideration damage to blood vessels 6 caused by the UFB jet water flow 43, this high-pressure pump section 22 has a function capable of adjusting the nozzle (pump) pressure during dispersion (separation) of the tissue mass 5 described below to approximately 0.5 to 10 MPa.

[0023] The ultra-high pressure pump unit 23 is a generating unit (high pressure jet water injection unit) for generating ultra-high pressure jet water from the buffer liquid 20 stored in the water tank unit 21. This ultra-high pressure pump unit 23 has a function of being able to adjust the nozzle pressure during extraction to about 10 to 20 MPa for cutting and recovering the remaining vascular cells 7, which are remnants of the tissue mass 5, as described below.

[0024] In this embodiment, the ultra-high pressure pump unit 23 and the pipe 3 are connected via pipe switching units 24 and 25.

[0025] FIG. 2 is a front view showing a schematic configuration of the tissue crushing unit 1. As shown in FIG.

[0026] 2, the texture grinding unit 1 is composed of a disposable part 10 and a nozzle forming part 40. The disposable part 10 has a cylindrical fixing metal fitting 11, a mesh-like mounting stage (filter stage) 12 as a mounting part on which the texture mass 5 is placed, and the like.

[0027] The mounting stage 12 is made of, for example, stainless steel and has a mesh size of about 20 to 100 mesh.

[0028] The disposable part 10 has an opening at the top, and a transparent sheet member 15 is attached and fixed by a fixing metal fitting 11 of the disposable part 10 so as to cover the opening. This makes it possible to prevent the UFB jet water flow 43 sprayed from the nozzle component 40 to the texture mass 5 during disassembly from splashing outside the disposable part 10.

[0029] That is, for example, the outer periphery of the sheet member 15 is fixed without any gaps to the edge of the opening of the disposable part 10 by the fixing metal fittings 11. Also, the sheet member 15 is provided at its approximately central position with an insertion part 15a through which the nozzle outlet part 42, which is the tip part of the nozzle head (nozzle part) 41, is inserted.

[0030] The sheet member 15 has an area sufficiently larger than the area of ​​the opening of the disposable part 10, and is adapted to be deformed appropriately in response to the movement of the nozzle head 41 without exposing the inside of the disposable part 10.

[0031] As shown in FIG. 1, the nozzle configuration unit 40 has a nozzle head 41, a holding unit 32 that holds the nozzle head 41, a horizontal movement mechanism 35 that moves the nozzle head 41 together with the holding unit 32 in the horizontal direction (the X direction shown in the figure), and a horizontal control unit 33 that controls the movement of the horizontal movement mechanism 35.

[0032] Nozzle head 41 is provided with air intake port 41h having a diameter of about 0.3 to 1 mm, which serves as a UFB generating section for generating UFB in the jet water stream.

[0033] The nozzle configuration unit 40 is also configured to have a vertical movement mechanism 30 that moves the nozzle head 41 together with the holding unit 32 along the rail 31 in the vertical direction (the Y direction shown in the figure), and a vertical control unit 34 that controls the movement of the vertical movement mechanism 30.

[0034] In this embodiment, the horizontal movement mechanism 35, the horizontal control section 33, the vertical movement mechanism 30, and the vertical control section 34 constitute a drive mechanism section.

[0035] During separation and extraction, the ejection port 42 is controlled by the vertical movement mechanism 30 and the vertical control unit 34 so that the distance between the ejection port 42 and the texture mass 5 is about 5 to 50 mm.

[0036] At the deepest part of the disposable section 10, a discharge port 18 with an opening / closing function (not shown) is provided for discharging unnecessary tissues other than the blood vessels 6, such as muscle and fat, of the tissue mass 5 to the outside.

[0037] Here, the configuration of the nozzle head 41 of the nozzle configuration section 40 in the cell dispersion device according to this embodiment will be described with reference to FIGS.

[0038] In the nozzle configuration section 40, a pipe 3 connected to the solution unit 2 is connected to the base end side of a nozzle head 41. A rectangular column-shaped nozzle port section 42 of different sizes is provided on the tip side of the nozzle head 41, as shown in Figures 3(a) and (b), for example. The nozzle port section 42 has a plurality of (here, 16) nozzle ports 42a, 42b arranged in a square shape of at least 4 x 4.

[0039] Of the multiple nozzles 42a, 42b, the inner nozzle 42b is arranged so that its opening area (diameter) is larger than that of the outer nozzle 42a, and the water flow of the sprayed UFB jet water flow water 43 is adjusted to be stronger on the outer side than on the inner side.

[0040] That is, by making the water pressure higher on the outside than on the inside, as shown in FIG. 4, for example, when the texture mass 5 is decomposed, the position of the texture mass 5 changes due to the inner water flow 43b when the texture mass 5 is pulverized, but this can be suppressed (fixed) by the outer water flow 43a.

[0041] For example, the diameter of the outer nozzle 42a of the nozzle section 42 is made smaller than the diameter of the inner nozzle 42b, so that the UFB jet water flow 43a is sprayed from the outer nozzle 42a at a water pressure at least twice as high as that of the inner nozzle. This makes it possible to prevent the tissue mass 5 and vascular cells 7 from flying out of the water flow 43 due to the shower pressure difference between the outer and inner jets.

[0042] It should be noted that the nozzle head 41 and the injection port portion 42 are not limited to being rectangular prism-shaped, but may be cylindrical or the like, and the openings of the injection ports 42a, 42b are not limited to being circular.

[0043] The diameter of the jet ports 42a, 42b is preferably, for example, about 0.8 to 3 mmφ, and the amount of water of the UFB jet water flow 43 is preferably, for example, about 0.5 to 3 L / min.

[0044] Next, the operation of the cell dispersion device according to this embodiment will be described.

[0045] FIG. 5 is shown to explain an example of the operation of the tissue crushing unit 1, where FIG. 5(a) shows the operation during separation and FIG. 5(b) shows the operation during extraction.

[0046] First, the process of separating the tissue mass 5 is carried out after optimizing the pressure of each part of the cell dispersion device, the amount of water, and the distance between the nozzle component 40 and the tissue mass 5 according to the tissue mass 5 to be crushed.

[0047] During separation, for example, as shown in FIG. 5(a), UFB jet water flow 43 (43a, 43b) is sprayed from the spray port 42 of the nozzle configuration part 40 onto a texture mass 5 of approximately 30 mm × 30 mm × 30 mm placed on a mounting stage 12.

[0048] At this time, the nozzle configuration unit 40 is repeatedly moved by the horizontal movement mechanism 35 and the horizontal control unit 33 in the direction of the arrow X in the drawing to a position at a predetermined height (Ya) from the mounting stage 12 with a predetermined stroke width. Also, a high-pressure UFB jet water stream pressurized to a predetermined pressure is supplied from the high-pressure pump unit 22. As a result, the high-pressure UFB jet water stream 43 (43a, 43b) is sprayed evenly over the entire texture mass 5.

[0049] In this manner, the high pressure UFB jet water 43a, 43b is ejected from the ejection port 42, thereby pulverizing the unnecessary tissue 5a of the tissue mass 5 other than the muscle and blood vessels 6. The unnecessary tissue 5a other than the pulverized muscle and blood vessels 6 passes through the mesh of the mounting stage 12 and is discharged to the outside from the discharge port 18 at the deepest part of the disposable section 10.

[0050] Unnecessary tissues 5a other than muscle and blood vessels 6 are crushed and removed from the tissue mass 5, and only blood vessels 6 and muscle cells 8 remain on the mounting stage 12 as residue, thereby completing the separation process and subsequently starting the extraction process.

[0051] During extraction, as shown in FIG. 5(c), a higher pressure UFB jet water flow 44 is then sprayed from the nozzle portion 42 of the nozzle configuration portion 40 onto the blood vessel 6 remaining on the mounting stage 12.

[0052] At that time, the nozzle component 40 is repeatedly moved in the direction of the arrow X shown in the figure by the horizontal movement mechanism 35 and the horizontal control unit 33 to a position (Yb) that is -Y lower than a predetermined height (Ya) from the mounting stage 12 with a predetermined stroke width.

[0053] Furthermore, a higher pressure UFB jet water flow 44 is supplied from the ultra-high pressure pump unit 23. This allows the higher pressure UFB jet water flow 44 to be sprayed evenly from closer positions throughout the entire blood vessel 6.

[0054] In this manner, with the nozzle 42 in close proximity, the UFB jet water 44 is sprayed at a higher water pressure to crush the blood vessels 6. Then, the vascular cells 7 are extracted from the crushed blood vessels 6 and are collected through the mesh of the mounting stage 12.

[0055] This makes it possible to easily pulverize human or animal tissue masses 5 without damaging the vascular cells 7, as shown in Figures 6(a) and (b), for example, and makes it easy to selectively extract the vascular cells 7.

[0056] In other words, according to the cell dispersion device of this embodiment, it is possible to efficiently separate and extract only the vascular cells 7 from the tissue mass 5 without finely chopping the tissue mass 5 with scissors and without damaging the vascular cells 7, and it is possible to easily ensure the number of vascular cells 7.

[0057] Figure 7 shows the results of the extraction process of liver blood vessels when the crushed subject was a mouse. As is clear from this micrograph, the collected tissue (DAPI positive cells) generally contains vascular (CD31) cells 7 as the main component.

[0058] Other embodiments 8-10 show a schematic configuration of a cell extraction device according to another embodiment of the present invention. Here, a configuration in which the position of the texture mass 5 is fixed by a mounting part having a double cage structure, rather than by the shower pressure difference shown in the first embodiment, will be described.

[0059] In this embodiment, the same or similar reference numerals are used for the same parts as those in the cell dispersion device of the above-mentioned embodiment, and only the different parts will be described.

[0060] The cell dispersion device of this embodiment, as shown in Figure 8, for example, comprises a tissue grinding unit 1, a solution unit 2, and piping 3 connecting a high-pressure pump section 22 or an ultra-high-pressure pump section 23 in the solution unit 2 to a nozzle component section 40 in the tissue grinding unit 1.

[0061] The tissue crushing unit 1 is a crusher for breaking down a tissue mass 5 excised from a living body to be crushed, using UFB jet water flow 43 as a jet of water, and extracting crushed vascular cells 7 from existing blood vessels 6, as shown in FIG. 9, for example.

[0062] In this embodiment, the texture grinding unit 1 is composed of a disposable part 10 and a nozzle forming part 40, as shown in Fig. 9 for example. The disposable part 10 comprises a cylindrical fixing metal fitting 11, a mesh-like mounting stage 12, a mesh-like cylindrical outer layer 13 which is mounted on the stage 12 and serves as an outer mesh in which the texture mass 5 is housed, and a mesh-like cylindrical inner layer 14 which serves as an inner mesh for fixing the position of the texture mass 5 housed in the mesh-like cylindrical outer layer 13.

[0063] The mounting stage 12, the mesh-tubular outer layer 13, and the mesh-tubular inner layer 14 are all made of, for example, stainless steel and have a mesh size of about 20 to 100 mesh.

[0064] In contrast, a plurality of nozzles (not shown) arranged in a square shape of at least 4 x 4 are opened in the nozzle outlet section 46, which is the tip part of the nozzle head (nozzle section) 41 constituting the nozzle configuration section 40. The opening areas (diameters) of the plurality of nozzles are all the same, and are adjusted so that the water flow of the sprayed UFB jet water flow 43 is almost uniform (even) on the inside and outside.

[0065] In the cell dispersion device of this embodiment, as shown in FIG. 10(a), for example, a tissue mass 5 to be crushed, which has been cut out from a living body or the like, is first placed in a mesh-like cylindrical outer layer 13 mounted on a mounting stage 12.

[0066] Next, the mesh tube inner layer 14 is set inside the mesh tube outer layer 13 on which the texture mass 5 is placed so as to apply a load to the texture mass 5 .

[0067] Then, after optimization of the pressure and the like, at the time of separation, a high-pressure UFB jet water flow 43 from the high-pressure pump section 22 is sprayed evenly over the entire texture mass 5 from the spray port section 46 of the nozzle configuration section 40 while moving horizontally at a position at a predetermined height (Ya) from the mounting stage 12 by a predetermined stroke width.

[0068] In this way, unnecessary tissue 5a other than muscle and blood vessels 6 of the tissue mass 5 is pulverized and removed, and then discharged to the outside from the discharge port 18 at the deepest part of the disposable section 10. After that, as shown in Figures 10(b) and 10(c), for example, the nozzle configuration section 40 together with the holding section 32 is moved to a position (Yb) lower than a predetermined height (Ya) from the mounting stage 12.

[0069] Then, the nozzle component 40 is repeatedly moved in the directions of the arrows X and -X shown in the figure by the horizontal movement mechanism 35 and the horizontal control unit 33 at a predetermined stroke width to a position (Yb) closer to the blood vessel 6, whereby the higher pressure UFB jet water flow 43 from the ultra-high pressure pump unit 23 is sprayed evenly over the entire blood vessel 6, which is the remaining material.

[0070] In addition, as the nozzle component 40 moves (descends), for example, the holding portion 32 is pushed down by the reticular tubular inner layer 14, and the reticular tubular outer layer 13 and the reticular tubular inner layer 14 hold the blood vessel 6 down so that it does not move.

[0071] In this way, by spraying the UFB jet water 43 at a higher water pressure against the pressed down blood vessel 6, the vascular cells 7 are extracted from the pulverized blood vessel 6 and collected through the mesh of the mounting stage 12.

[0072] According to the cell dispersion device of this embodiment, it is possible to efficiently separate and extract only the crushed vascular cells 7 from the tissue mass 5 without finely chopping the tissue mass 5 with scissors and without damaging the vascular cells 7, and it is possible to easily ensure the number of crushed vascular cells 7.

[0073] Moreover, regardless of the shower pressure difference between the outside and inside of the UFB jet water flow 43 ejected from the ejection port 46, it is possible to prevent the tissue mass 5 and blood vessels 6 from flying out due to the ejection of the UFB jet water flow 43.

[0074] Alternative Embodiments The cell dispersion device of this embodiment is an example of a configuration including the injection port portion 42 shown in one embodiment and the mesh-like cylindrical outer layer 13 and mesh-like cylindrical inner layer 14 shown in another embodiment, as shown in FIG.

[0075] That is, the texture mass 5 to be pulverized is pressed down by the reticulated cylindrical inner layer 14 set within the reticulated cylindrical outer layer 13, and is separated by UFB jet water flows 43a, 43b adjusted so that the water flow from the nozzle 42 is stronger on the outside than on the inside.

[0076] With the configuration of this embodiment, it is possible to efficiently separate and extract only the crushed vascular cells 7 from the tissue mass 5 without finely chopping the tissue mass 5 with scissors and without damaging the vascular cells 7, making it possible to easily ensure the number of crushed vascular cells 7 and easily prevent the tissue mass 5 and the like from jumping out of the water flow.

[0077] As described above, in any of the embodiments, when performing tissue culture or cell culture, it is possible to isolate only vascular cells from a tissue mass without damaging the vascular cells, and it is also easy to ensure the number of vascular cells to be cultured.

[0078] It is not necessary to provide a separate, dedicated ultra-high pressure pump unit 23, and the high pressure pump unit 22 may be configured with a pressure switching function that can adjust the pump pressure to about 0.5 to 10 MPa during separation to disperse the blood vessels 6 from the tissue mass 5, and to about 10 to 2 MPa during extraction to cut the separated blood vessels 6, which are the remains of the tissue mass 5, and collect the vascular cells 7.

[0079] In this case, the high-pressure jet water ejection section is constituted by the high-pressure pump section 22 in a state in which the pump pressure is switched to about 10 to 20 MPa for extraction by the pressure switching function.

[0080] The above describes the aspects of the present invention by illustrating the embodiment, but this is only an example, and the scope of the invention described in the claims can be modified in various ways without departing from the gist of the invention. [Explanation of symbols]

[0081] 1 Tissue grinding unit 2 Solution Unit 5 Tissue mass 6 Blood vessels (tissues) 7. Crushed vascular cells 10 Disposables Section 11 Fixing bracket 12 Mounting stage (mounting part) 13 Reticular cylinder outer layer (outer mesh) 14 Inner layer of the mesh tube (inner mesh) 20 Buffer Solution 21 Aquarium section 22 High pressure pump section (generation section) 23 Ultra-high pressure pump section (generation section / high pressure jet water injection section) 30 Vertical movement mechanism 35 Horizontal movement mechanism 40 Nozzle component 41 Nozzle head (nozzle part) 41h Air intake (UFB generation section) 42,46 Injection port 42a,42b injection port 43 (43a, 43b), 44 UFB jet water (jet water)

Claims

1. A cell extraction device capable of selectively extracting cells from a tissue mass, comprising: a mesh-like placement portion on which the texture mass is placed; a nozzle unit that injects a jet of water onto the tissue mass placed on the placement unit to separate the cells from the tissue mass; a generating unit that generates the jet water to be sprayed from the nozzle unit; a high-pressure jet water injection unit that injects, from the nozzle unit, the jet water generated in the generation unit and at a higher pressure onto the remainder of the texture mass onto which the jet water has been injected; A cell extraction device comprising:

2. 2. The cell extraction device according to claim 1, wherein blood vessel cells are extracted as the cells.

3. 2. The cell extraction device according to claim 1, further comprising a drive mechanism that holds the nozzle section so that the nozzle section is movable vertically and horizontally relative to the mounting section.

4. 2. The cell extraction device according to claim 1, wherein the generating unit generates the jet water from a physiological saline solution or a buffer solution.

5. 2. The cell extraction device according to claim 1, wherein the nozzle unit ejects a jet of flowing water containing ultra-fine bubbles.

6. The cell extraction device according to claim 1, characterized in that the nozzle portion has a plurality of nozzles arranged in a square of at least 4 x 4, and among the plurality of nozzles, the inner nozzles have a larger opening area than the outer nozzles.

7. 2. The cell extraction device according to claim 1, wherein the placement section comprises an outer mesh that contains the tissue mass, and an inner mesh that fixes a position of the tissue mass contained within the outer mesh.

8. 2. The cell extraction device according to claim 1, wherein the generating unit includes a high-pressure pump that generates the jet water having a pump pressure of about 0.5 to 10 MPa to be sprayed onto the tissue mass placed on the placement unit, and an ultra-high pressure pump that generates jet water having a pump pressure of about 10 to 20 MPa and a higher pressure than the jet water to be sprayed onto remains of the tissue mass, the ultra-high pressure pump forming the high-pressure jet water spraying unit.

9. A cell extraction method capable of selectively extracting cells from a tissue mass, comprising: placing the texture mass on a mesh-like placement section; generating a jet of water to be sprayed onto the texture mass by a generating unit; spraying the jet water generated in the generating unit from a nozzle unit against the texture mass placed on the placing unit; a step of injecting, from the nozzle portion, jet water of higher pressure generated in the generating portion, onto the remainder of the texture mass onto which the jet water has been injected, by a high-pressure jet water injecting portion; Equipped with A cell extraction method, comprising: switching the pressure of the jet water sprayed from the nozzle portion to separate the cells from the tissue mass.

Images