Biosensor, multi-imposed sheet, and method of manufacturing biosensor

Abstract

To provide a biosensor capable of improving electrical characteristics.SOLUTION: A biosensor includes: an insulating substrate 2; a working electrode 10, a counter electrode 11, and a connecting electrode 12 provided on the insulating substrate 2; an insulating layer 20 provided on the insulating substrate 2 so as to insulate the working electrode 10, the counter electrode 11, and the connecting electrode 12 from one another and having an opening 23 that partially exposes the connecting electrode 12; and a reference electrode 30 provided within the opening 23 and on the connecting electrode 12 so as to partially overlap the insulating layer 20. The thickness of the insulating layer 20 is 10 μm or more and 30 μm or less.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a biosensor, a multi-imposition sheet, and a method for manufacturing a biosensor. [Background technology]

[0002] In recent years, biosensors have been widely used in fields such as medicine. Biosensors are capable of measuring the properties of a sample solution by utilizing an electrochemical reaction. The biosensor includes a working electrode, a counter electrode, and a reference electrode obtained by patterning a conductive material on an insulating substrate, and further includes an insulating layer that insulates the working electrode, the counter electrode, and the reference electrode from each other. The working electrode is an electrode for detecting the object to be measured. A predetermined potential difference is applied between the working electrode and the counter electrode, and the potential of the reference electrode is used as a reference potential during measurement.

[0003] A biomaterial (enzyme) that reacts with a specific substrate is immobilized on the working electrode. The substrate concentration can be measured by measuring the current value obtained by the electrochemical reaction between the biomaterial and a specific component (substrate) contained in the sample solution to be measured.

[0004] The electrodes constituting the biosensor can be formed, for example, by a printing method. If the electrode pattern formed by the printing method is not a uniform film, the electrical characteristics of the biosensor will deteriorate. In particular, if the potential of the reference electrode is not stable, the current value between the working electrode and the counter electrode may not be measured accurately. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] JP 2007-278981 A [Patent Document 2] JP 2007-309912 A Summary of the Invention [Problem to be solved by the invention]

[0006] The present invention provides a biosensor capable of improving electrical characteristics, a multi-imposed sheet, and a method for manufacturing a biosensor. [Means for solving the problem]

[0007] According to a first aspect of the present invention, there is provided a biosensor comprising an insulating substrate, a working electrode, a counter electrode, and a connecting electrode provided on the insulating substrate, an insulating layer provided on the insulating substrate so as to insulate the working electrode, the counter electrode, and the connecting electrode from one another, the insulating layer having an opening that partially exposes the connecting electrode, and a reference electrode provided on the connecting electrode and in the opening so as to partially overlap the insulating layer, wherein the insulating layer has a thickness of 10 μm or more and 30 μm or less.

[0008] According to a second aspect of the present invention, there is provided the biosensor according to the first aspect, wherein the reference electrode has a thickness of 5 μm or more and 15 μm or less.

[0009] According to a third aspect of the present invention, there is provided the biosensor according to the first aspect, wherein the reference electrode is constituted by a silver-silver chloride electrode.

[0010] According to a fourth aspect of the present invention, there is provided the biosensor according to the first aspect, wherein the insulating layer is made of a resin.

[0011] According to a fifth aspect of the present invention, there is provided the biosensor according to the first aspect, wherein the working electrode, the counter electrode and the connecting electrode are made of carbon paste.

[0012] According to a sixth aspect of the present invention, there is provided a biosensor according to the first aspect, further comprising a first wiring, a second wiring, and a third wiring provided on the insulating substrate and electrically connected to the working electrode, the counter electrode, and the connecting electrode, respectively, and the insulating layer is further provided on the first wiring, the second wiring, and the third wiring.

[0013] According to a seventh aspect of the present invention, there is provided a multi-faceted sheet comprising a plurality of electrode chips provided on a sheet-like insulating substrate, each of the plurality of electrode chips being the biosensor according to the first aspect.

[0014] According to an eighth aspect of the present invention, there is provided a method for manufacturing a biosensor, the method comprising the steps of: forming a working electrode, a counter electrode, and a connecting electrode on an insulating substrate by screen printing; forming an insulating layer on the insulating substrate by screen printing, the insulating layer having an opening that partially exposes the connecting electrode so as to insulate the working electrode, the counter electrode, and the connecting electrode from one another; and forming a reference electrode on the connecting electrode and in the opening by screen printing so as to partially overlap the insulating layer, wherein the insulating layer has a thickness of 10 μm or more and 30 μm or less.

[0015] According to a ninth aspect of the present invention, there is provided the method for producing the biosensor according to the eighth aspect, wherein the reference electrode has a thickness of 5 μm or more and 15 μm or less.

[0016] According to a tenth aspect of the present invention, there is provided a method for producing a biosensor according to the eighth aspect, wherein the reference electrode is formed using a silver-silver chloride paste.

[0017] According to an eleventh aspect of the present invention, there is provided a method for producing a biosensor according to the tenth aspect, wherein the viscosity of the silver-silver chloride paste is 5 Pa·s or more and 20 Pa·s or less.

[0018] According to a twelfth aspect of the present invention, there is provided a method for manufacturing a biosensor according to the eighth aspect, wherein the insulating layer is formed using an insulating paste, and the viscosity of the insulating paste is 10 Pa·s or more and 80 or less.

[0019] According to a thirteenth aspect of the present invention, there is provided a method for producing a biosensor according to the eighth aspect, wherein the working electrode, the counter electrode and the connecting electrode are formed using carbon paste. Effect of the Invention

[0020] According to the present invention, it is possible to provide a biosensor, a multi-imposed sheet, and a method for manufacturing a biosensor that are capable of improving electrical characteristics. [Brief description of the drawings]

[0021] [Figure 1] FIG. 1 is a plan view of a biosensor according to an embodiment of the present invention. [Diagram 2] FIG. 2 is an exploded plan view of the layers that make up the biosensor. [Diagram 3] FIG. 3 is a cross-sectional view of the biosensor taken along line AA' in FIG. [Figure 4] FIG. 4 is a plan view illustrating the manufacturing process of the biosensor. [Diagram 5] FIG. 5 is a cross-sectional view illustrating the manufacturing process of the biosensor taken along the line AA′ in FIG. [Figure 6] FIG. 6 is a plan view illustrating the manufacturing process of the biosensor. [Figure 7] 7A to 7C are cross-sectional views illustrating the manufacturing process of the biosensor taken along the line AA′ in FIG. [Figure 8] FIG. 8 is a plan view of the multi-imposition sheet. [Figure 9] FIG. 9 is a cross-sectional view illustrating a manufacturing process of a biosensor according to a comparative example. [Figure 10] FIG. 10 is a cross-sectional view illustrating a manufacturing process of a biosensor according to a comparative example. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0022] Hereinafter, the embodiments will be described with reference to the drawings. However, the drawings are schematic or conceptual, and the dimensions and ratios of each drawing are not necessarily the same as those in reality. In addition, even when the same parts are shown between the drawings, the dimensional relationships and ratios of each may be different. In particular, the following embodiments are examples of devices and methods for embodying the technical idea of ​​the present invention, and the technical idea of ​​the present invention is not specified by the shape, structure, arrangement, etc. of the components. In the following description, elements having the same function and configuration are given the same reference numerals, and duplicated descriptions are omitted.

[0023] [1] Configuration of biosensor 1 FIG. 1 is a plan view of a biosensor 1 according to an embodiment of the present invention. In FIG. 1, the X direction is a direction along one side of the biosensor 1, and the Y direction is a direction perpendicular to the X direction in a plane. FIG. 2 is a plan view in which a plurality of layers constituting the biosensor 1 are exploded. FIG. 2(a) is a plan view of a first electrode pattern (including a working electrode 10 and a counter electrode 11). FIG. 2(b) is a plan view of an insulating layer pattern. FIG. 2(c) is a plan view of a second electrode pattern (including a reference electrode 30). The first electrode pattern of FIG. 2(a), the insulating layer pattern of FIG. 2(b), and the second electrode pattern of FIG. 2(c) are laminated in this order. FIG. 3 is a cross-sectional view of the biosensor 1 taken along the line AA′ of FIG. 1.

[0024] The biosensor 1 comprises an insulating substrate 2 , a working electrode 10 , a counter electrode 11 , a connecting electrode 12 , an insulating layer 20 , and a reference electrode 30 .

[0025] The insulating substrate 2 is made of an insulating material such as plastic, glass, ceramic, etc. Examples of the plastic include polyethylene terephthalate (PET), polyester, polystyrene, polypropylene, polycarbonate, polyimide, and acrylic resin.

[0026] A working electrode 10 is provided on the insulating substrate 2. The working electrode 10 is an electrode for detecting a measurement target. A substance that undergoes an electrochemical reaction (oxidation or reduction) with a sample solution to be measured is fixed on the working electrode 10. The working electrode 10 has, for example, a circular shape. The working electrode 10 is made of a conductive material, such as metal, carbon black, or graphite. The working electrode 10 may be made of a conductive paste containing conductive particles and an adhesive. From the viewpoints of corrosion resistance and cost, it is desirable to make the working electrode 10 of a carbon paste. The carbon paste contains carbon and an adhesive such as a resin. The carbon contains carbon black, graphite, or a mixture of carbon black and graphite.

[0027] A counter electrode 11 is provided on the insulating substrate 2. The counter electrode 11 is an electrode that forms a pair with the working electrode 10, and is an electrode for passing a current generated in the working electrode 10. The counter electrode 11 is disposed at a distance from the working electrode 10 so as to surround a part of the periphery of the working electrode 10. In this embodiment, the counter electrode 11 is configured to be along approximately half of the periphery of the working electrode 10. The counter electrode 11 can be made of the same conductive material as the working electrode 10. Alternatively, the counter electrode 11 may be made of a conductive material different from that of the working electrode 10.

[0028] A connection electrode 12 is provided on the insulating substrate 2. The connection electrode 12 is an electrode that is electrically connected to the reference electrode 30. The connection electrode 12 is disposed adjacent to the working electrode 10 in a region other than the region in which the counter electrode 11 is disposed. The connection electrode 12 has a rectangular shape extending in the X direction. The connection electrode 12 may be made of the same conductive material as the working electrode 10. Alternatively, the connection electrode 12 may be made of a conductive material different from that of the working electrode 10.

[0029] Wiring 13-15 are provided on insulating substrate 2. Wiring 13 is electrically connected to working electrode 10. Wiring 13 is made of the same conductive material as working electrode 10. Wiring 14 is electrically connected to connection electrode 12. Wiring 14 is made of the same conductive material as connection electrode 12. Wiring 15 is electrically connected to counter electrode 11. Wiring 15 is made of the same conductive material as counter electrode 11. Wirings 13-15 are each configured to extend in the Y direction.

[0030] An insulating layer 20 is provided on the insulating substrate 2, the connection electrodes 12, and the wiring 13-15. The insulating layer 20 is made of an insulating material, for example, a resin. Examples of the resin include epoxy resin and phenol resin. The insulating layer 20 is desirably made of a resin that can be formed in a printing process, and is desirably made of a thermosetting resin or a UV (ultraviolet) curing resin. The insulating layer 20 includes a first portion 21 for insulating the working electrode 10 and the counter electrode 11, a second portion 22 for covering the wiring, and a plurality of openings 23-26.

[0031] The first portion 21 of the insulating layer 20 is configured to surround the working electrode 10 and is configured in a circular ring shape. The first portion 21 is disposed between the working electrode 10 and the counter electrode 11, and insulates the working electrode 10 from the counter electrode 11. The first portion 21 has a function of preventing a substance immobilized on the working electrode 10 from contacting the counter electrode 11.

[0032] The second portion 22 of the insulating layer 20 is configured to cover the connection electrode 12 and the wirings 13 to 15. The second portion 22 is provided to insulate the wirings 13 to 15.

[0033] The opening 23 partially exposes the connection electrode 12. The opening 24 partially exposes the end of the wiring 13. The opening 25 partially exposes the end of the wiring 14. The opening 26 partially exposes the end of the wiring 15. The three wiring portions of the wirings 13 to 15 exposed by the openings 24 to 26, respectively, are used as three terminals T1 to T3 of the biosensor 1.

[0034] A reference electrode 30 is provided on the connection electrode 12. The reference electrode 30 has a rectangular shape extending in the X direction in a plan view. The reference electrode 30 is provided in the opening 23 and on the connection electrode 12 so as to partially overlap the insulating layer 20. In other words, the reference electrode 30 is provided in the opening 23 and on the connection electrode 12 so that both ends in the X direction overlap the insulating layer 20. The reference electrode 30 is an electrode that generates a reference potential. The reference electrode 30 is preferably made of a conductive material, and is preferably made of a material whose potential is stable when a current flows through it. The reference electrode 30 is, for example, made of a silver-silver chloride electrode.

[0035] This is how the biosensor 1 is constructed. The terminals T1 to T3 of the biosensor 1 are connected to connectors of an electrochemical detection device.

[0036] In the method of using the biosensor 1, a mediator layer is deposited on the working electrode 10. The mediator layer functions as an electron transfer material. For example, an oxidoreductase is deposited on the mediator layer as a reactant such as a biological material. For example, a protective film (not shown) is formed on the oxidoreductase. When the biosensor 1 is used, a predetermined potential difference is applied between the counter electrode 11 and the working electrode 10 based on the potential of the reference electrode 30. The sample solution that has permeated the protective film reacts with the oxidoreductase to generate a target substance. As a result, a current corresponding to the concentration of the target substance flows between the working electrode 10 and the counter electrode 11. The electrochemical detection device measures the value of the current flowing between the working electrode 10 and the counter electrode 11, and can measure the concentration of the target substance in the sample solution based on the measurement result.

[0037] The biosensor 1 of this embodiment is capable of measuring, for example, the blood glucose level or the lactate level in a biological sample.

[0038] [2] Manufacturing method Next, a method for manufacturing the biosensor 1 will be described with reference to the drawings. Figures 4 and 6 are plan views illustrating the manufacturing process of the biosensor 1. Figures 5 and 7 are cross-sectional views illustrating the manufacturing process of the biosensor 1.

[0039] First, the insulating substrate 2 is prepared. The insulating substrate 2 is made of, for example, a PET film. Next, the PET film serving as the insulating substrate 2 is heat-treated (annealed). In this heat treatment, heat equal to or higher than the heat treatment temperature of the subsequent process is applied so that the dimensions do not change due to the thermal history of the subsequent process. Next, holes for print stage positioning and print alignment are formed in the insulating substrate 2 using a die-cutting blade.

[0040] Next, a screen printer for screen printing is prepared. Alignment pins are provided on the stage of the screen printer. Next, the insulating substrate 2 is set on the stage so that the pins of the stage are inserted into the holes of the insulating substrate 2, and the insulating substrate 2 is vacuum-adsorbed to the stage.

[0041] 4 and 5, a working electrode 10, a counter electrode 11, a connection electrode 12, and wirings 13 to 15 are simultaneously formed on the insulating substrate 2 by screen printing. Specifically, a screen printing plate having an electrode pattern is used to screen print a conductive paste on the insulating substrate 2. Carbon paste is used as the conductive paste. The carbon paste is then cured. In this curing step, the carbon paste is cured by heating or UV irradiation. As a result, an electrode pattern including the working electrode 10, the counter electrode 11, the connection electrode 12, and wirings 13 to 15 is formed on the insulating substrate 2.

[0042] 6 and 7, an insulating layer 20 that insulates the working electrode 10, the counter electrode 11, and the connection electrode 12 from one another is formed by screen printing. The insulating layer 20 includes a first portion 21 that insulates the working electrode 10, the counter electrode 11, and the connection electrode 12 from one another, and a second portion 22 that covers the wirings 13 to 15. Furthermore, the insulating layer 20 has an opening 23 that partially exposes the connection electrode 12, and openings 24 to 26 that partially expose the wirings 13 to 15, respectively.

[0043] Specifically, an insulating paste is screen-printed on the insulating substrate 2 and the electrodes using a screen printing plate on which a pattern for the insulating layer 20 is formed. As the insulating paste, a thermosetting resin or a UV-curing resin is used. As the insulating paste, a thermosetting type insulating paste that does not contain a main solvent of the carbon paste, or a solvent-free UV-curing type insulating paste is preferable. As the thermosetting type insulating paste, a material containing an epoxy resin and a hardener is used. As the UV-curing type insulating paste, a material containing an epoxy resin and a photopolymerization initiator is used. Then, the insulating paste is cured. In this curing process, the paste is cured by heating or UV irradiation. As a result, the insulating layer 20 (including the first portion 21 and the second portion 22) is formed on the insulating substrate 2 and the electrodes.

[0044] In this embodiment, the thickness of the insulating layer 20 is reduced in order to reduce the smearing of the reference electrode 30 formed in the opening 23 of the insulating layer 20. The smearing is a state in which the film is not continuous due to breaks or holes, and the film properties are deteriorated.

[0045] The lower limit of the thickness of the insulating layer 20 is preferably 10 μm or more, and more preferably 15 μm or more. The upper limit of the thickness of the insulating layer 20 is preferably 30 μm or less, and more preferably 25 μm or less.

[0046] The viscosity of the insulating paste for insulating layer 20 is preferably 10 Pa·s (pascal seconds) or more and 80 or less. By reducing the viscosity of the insulating paste for insulating layer 20, the film thickness of insulating layer 20 can be made thinner.

[0047] The screen printing plate for forming the insulating layer 20 preferably has a mesh number of 250 or more and a mesh line diameter of 30 μm or less. The mesh number is the number of threads (total number of warp threads and weft threads) per inch. By using such a screen printing plate, it is possible to make the film thickness of the insulating layer 20 thinner.

[0048] Next, as shown in Figs. 1 and 3, a reference electrode 30 is formed on the connection electrode 12 by using a screen printing method. Specifically, a conductive paste is screen-printed on the connection electrode 12 and in the opening 23 of the insulating layer 20 using a screen printing plate on which a pattern for the reference electrode is formed. A paste containing silver chloride as a main component (referred to as silver chloride paste) is used as the conductive paste. Next, the silver chloride paste is thermally cured. The film thickness of the reference electrode 30 is preferably 5 µm or more and 15 µm or less. The film thickness of the reference electrode 30 is the film thickness on the connection electrode 12.

[0049] The silver-silver chloride paste contains silver particles, silver chloride particles, a thermoplastic resin, and a solvent. The viscosity of the silver-silver chloride paste is preferably 5 Pa·s or more and 20 Pa·s or less. In addition, since the silver-silver chloride paste dries easily, it is preferable to use a solvent with a high boiling point to make the silver-silver chloride paste difficult to dry. The solvent contained in the silver-silver chloride paste preferably has a boiling point of 200°C or more and a vapor pressure of 30 Pa or less, for example.

[0050] Since the silver-silver chloride paste is susceptible to corrosion, it is preferable to use a non-metallic mesh (such as a polyester mesh) for the screen printing plate in order to suppress corrosion. Also, in order to suppress corrosion of the silver-silver chloride paste, it is preferable to make the scraper used in the screen printing out of a material other than metal (including resin).

[0051] As a result, the reference electrode 30 is formed on the connection electrode 12 and in the opening 23 of the insulating layer 20. The reference electrode 30 is formed in the opening 23 and on the connection electrode 12 so as to partially overlap the insulating layer 20. The manufacturing method of this embodiment can prevent the reference electrode 30 from being smudged. In addition, the reference electrode 30 can be formed as a continuous film.

[0052] In this manner, the biosensor 1 according to this embodiment is manufactured.

[0053] [3] Composition of multi-imposition sheet 40 Next, the configuration of the multi-imposition sheet 40 will be described.

[0054] Fig. 8 is a plan view of the multi-imposed sheet 40. The multi-imposed sheet 40 includes a plurality of electrode chips 41. Fig. 8 shows 56 (=8×7) electrode chips 41 as an example. The electrode chips 41 correspond to the biosensor 1 described above. In Fig. 8, one electrode chip 41 is shown simplified as a rectangle.

[0055] The electrode chips 41 are provided on a sheet-like insulating substrate 2. The electrode chips 41 are manufactured using the same screen printing process, which reduces the manufacturing cost of the electrode chips 41.

[0056] The multi-faceted sheet 40 having a plurality of electrode chips 41 attached thereto is cut into individual electrode chips 41 .

[0057] [4] Comparative example Next, the configuration of a biosensor according to a comparative example will be described.

[0058] Fig. 9 is a cross-sectional view for explaining a manufacturing process of a biosensor according to a comparative example. The plan view corresponding to Fig. 9 is the same as Fig. 6. In the comparative example, the film thickness of the insulating layer 20 is different from that of the embodiment.

[0059] Using a screen printing plate on which a pattern for the insulating layer 20 is formed, an insulating paste is screen printed on the insulating substrate 2 and the electrodes to form the insulating layer 20. The thickness of the insulating layer 20 is, for example, about 40 μm. The thickness of 40 μm is the thickness of the insulating layer on the electrodes. The screen printing plate for forming the insulating layer 20 has a mesh number of 200 and a mesh line diameter of 40 μm.

[0060] 10 is a cross-sectional view illustrating a manufacturing process of a biosensor according to a comparative example, and the plan view corresponding to FIG.

[0061] Using a screen printing plate on which a pattern for the reference electrode is formed, a conductive paste is screen-printed onto the connection electrode 12 and within the opening 23 of the insulating layer 20 to form the reference electrode 30.

[0062] In the comparative example, the insulating layer 20 is thick, so that the step on the connection electrode 12 is large. This causes the reference electrode 30 on the connection electrode 12 to be scratched. Specifically, the connection electrode 12 has a plurality of holes and is divided at a plurality of points. As a result, the electrical characteristics of the biosensor according to the comparative example are deteriorated.

[0063] On the other hand, in this embodiment, the insulating layer 20 is formed to be thin. Also, a method is used in which the reference electrode 30 is a continuous film. As a result, the biosensor 1 of this embodiment can have improved electrical characteristics compared to the comparative example.

[0064] [5] Effects of the embodiment According to this embodiment, the biosensor 1 can be manufactured using a screen printing process, thereby reducing the manufacturing cost of the biosensor 1.

[0065] Furthermore, the insulating layer 20 can be formed thinly using a screen printing process. This allows the step of the opening 23 for the reference electrode 30 in the insulating layer 20 to be small. This allows the silver-silver chloride paste used as the reference electrode 30 to be printed without smearing when it is formed by screen printing.

[0066] Furthermore, the reference electrode 30 can be made of a continuous film. This makes it possible to stabilize the potential generated by the reference electrode 30. As a result, the electrical characteristics of the biosensor 1 can be improved.

[0067] Furthermore, a screen printing process can be used to form a plurality of biosensors 1 on multiple surfaces, thereby reducing the manufacturing cost of the biosensors 1. Furthermore, mass production of the biosensors 1 is possible, and disposable biosensors 1 can be manufactured at low cost.

[0068] Furthermore, by manufacturing the biosensor 1 using the manufacturing method of this embodiment, the defective rate of the biosensor 1 can be reduced during mass production.

[0069] The present invention is not limited to the above-mentioned embodiment, and various modifications can be made in the implementation stage without departing from the gist of the invention. In addition, the embodiments may be implemented in appropriate combination, and in that case, the combined effect can be obtained. Furthermore, the above-mentioned embodiment includes various inventions, and various inventions can be extracted by combinations selected from the multiple components disclosed. For example, if the problem can be solved and the effect can be obtained even if some components are deleted from all the components shown in the embodiment, the configuration from which the components are deleted can be extracted as an invention. [Explanation of symbols]

[0070] 1...biosensor, 2...insulating substrate, 10...working electrode, 11...counter electrode, 12...connecting electrode, 13-15...wiring, 20...insulating layer, 23-26...opening, 30...reference electrode, 40...multiple-sided attachment sheet, 41...electrode chip, T1-T3...terminals.

Claims

1. An insulating substrate; A working electrode, a counter electrode, and a connection electrode provided on the insulating substrate; an insulating layer provided on the insulating substrate so as to insulate the working electrode, the counter electrode, and the connecting electrode from one another, the insulating layer having an opening for partially exposing the connecting electrode; a reference electrode provided on the connection electrode and within the opening so as to partially overlap the insulating layer; Equipped with The thickness of the insulating layer is 10 μm or more and 30 μm or less. Biosensors.

2. The film thickness of the reference electrode is 5 μm or more and 15 μm or less. The biosensor according to claim 1 .

3. The reference electrode is a silver-silver chloride electrode. The biosensor according to claim 1 .

4. The insulating layer is made of a resin. The biosensor according to claim 1 .

5. The working electrode, the counter electrode, and the connecting electrode are made of carbon paste. The biosensor according to claim 1 .

6. a first wiring, a second wiring, and a third wiring provided on the insulating substrate and electrically connected to the working electrode, the counter electrode, and the connection electrode, respectively; The insulating layer is further provided on the first wiring, the second wiring, and the third wiring. The biosensor according to claim 1 .

7. The device includes a plurality of electrode chips provided on a sheet-like insulating substrate, Each of the plurality of electrode chips is a biosensor according to claim 1. Multi-sided sheet.

8. forming a working electrode, a counter electrode, and a connection electrode on an insulating substrate by screen printing; forming an insulating layer having openings that partially expose the connection electrodes on the insulating substrate by using a screen printing method so as to insulate the working electrode, the counter electrode, and the connection electrodes from one another; forming a reference electrode on the connection electrode and in the opening so as to overlap the insulating layer by a screen printing method; Equipped with The thickness of the insulating layer is 10 μm or more and 30 μm or less. A method for manufacturing a biosensor.

9. The film thickness of the reference electrode is 5 μm or more and 15 μm or less. A method for producing the biosensor according to claim 8 .

10. The reference electrode is formed using silver-silver chloride paste. A method for producing the biosensor according to claim 8 .

11. The viscosity of the silver-silver chloride paste is 5 Pa·s or more and 20 Pa·s or less. The method for producing the biosensor according to claim 10 .

12. The insulating layer is formed using an insulating paste, The viscosity of the insulating paste is 10 Pa·s or more and 80 Pa·s or less. A method for producing the biosensor according to claim 8 .

13. The working electrode, the counter electrode, and the connecting electrode are formed using carbon paste. A method for producing the biosensor according to claim 8 .

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