Method of producing processed ascidian, and processed ascidian

Abstract

To provide: a method of producing a processed ascidian having good temporal stability of contained plasmalogen; and a processed ascidian where the temporal stability of contained plasmalogen is improved.SOLUTION: The invention provides: a method of producing a processed ascidian having good temporal stability of contained plasmalogen by freeze-drying Halocynthia roretzi or another ascidian without encystment; and a processed product of Halocynthia roretzi or another ascidian without encystment, where the temporal stability of contained plasmalogen is improved by freeze drying.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a method for manufacturing processed products of mabooya or other sea squirts and processed sea squirt products.

Background Art

[0002] Japanese Patent Application Laid-Open No. 2016-210696 discloses a learning and memory ability enhancer for use in healthy mammals, which contains plasmalogen, and more than 90% of the plasmalogen is ethanolamine plasmalogen and choline plasmalogen. The plasmalogen is extracted from avian tissues. Preferably, the mass ratio of (ethanolamine plasmalogen: choline plasmalogen) contained in the plasmalogen is (1:5) to (5:1).

[0003] Japanese Patent Application Laid-Open No. 2019-162042 discloses an invention related to an extract from seafood or aquatic animals, which contains a high purity of plasmalogen-type phospholipids and has a reduced arsenic content, and a method for manufacturing the same.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0005] An object of the present invention is to provide a method for manufacturing a processed sea squirt product having good stability of plasmalogen over time and a processed sea squirt product having improved stability of plasmalogen over time.

Means for Solving the Problems

[0006] The method for manufacturing a processed jellyfish product and the processed jellyfish product of the present invention can be represented as follows.

[0007] (1) A method for manufacturing a processed jellyfish product having good temporal stability of the contained plasmalogen, characterized in that freeze-drying is performed on maboya or other jellyfish excluding the capsule. A method for manufacturing a processed jellyfish product, characterized in that freeze-drying is performed on maboya or other jellyfish excluding the capsule.

[0008] (2) The manufacturing method according to (1) above, wherein before performing the freeze-drying, maboya or other jellyfish excluding the capsule is treated with hot water.

[0009] (3) The manufacturing method according to (1) or (2) above, wherein substantially no substance other than water is added to the maboya or other jellyfish.

[0010] (4) The manufacturing method according to any one of (1) to (3) above, wherein the plasmalogen content in a predetermined mass of the processed jellyfish product after freeze-drying is 50% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 25°C.

[0011] (5) The manufacturing method according to any one of (1) to (3) above, wherein the plasmalogen content in a predetermined mass of the processed jellyfish product after freeze-drying is 40% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 35°C.

[0012] (6) The manufacturing method according to any one of (1) to (3) above, wherein the plasmalogen content in a predetermined mass of the processed jellyfish product after freeze-drying is 40% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 6 months in an environment of 25°C.

[0013] (7) The method of manufacturing according to any one of (1) to (3) above, wherein the content of plasmalogen in a predetermined mass of the processed jellyfish product after freeze-drying is 30% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 6 months have elapsed in an environment of 35°C.

[0014] (8) A processed product of maboya or other jellyfish excluding the capsule, characterized in that the stability over time of the plasmalogen contained therein is improved by freeze-drying.

[0015] (9) The processed jellyfish product according to (8) above, which substantially contains no substances other than water in addition to the said maboya or other jellyfish.

[0016] (10) The processed jellyfish product according to (8) or (9) above, wherein the content of plasmalogen in a predetermined mass after freeze-drying is 30% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 2 months have elapsed in an environment of 25°C.

[0017] (11) The processed jellyfish product according to (8) or (9) above, wherein the content of plasmalogen in a predetermined mass after freeze-drying is 20% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 2 months have elapsed in an environment of 35°C.

[0018] (12) The processed jellyfish product according to (8) or (9) above, wherein the content of plasmalogen in a predetermined mass after freeze-drying is 20% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 6 months have elapsed in an environment of 25°C.

[0019] (13) The processed jellyfish product according to (8) or (9) above, wherein the content of plasmalogen in a predetermined mass after freeze-drying is 10% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 6 months have elapsed in an environment of 35°C.

[0020] (14) The processed sea squirt product according to the above (8) or (9), wherein after freeze-drying and after more than 2 months have elapsed at room temperature, the plasmaogen content in a predetermined mass is 50% or more of the plasmaogen content in the said predetermined mass immediately after freeze-drying.

[0021] (15) The processed sea squirt product according to the above (8) or (9), wherein after freeze-drying and after more than 2 months have elapsed at room temperature, the plasmaogen content in a predetermined mass is 30% or more of the plasmaogen content in the said predetermined mass immediately after freeze-drying.

[0022] (16) The processed sea squirt product according to the above (8) or (9), wherein after freeze-drying and after more than 2 months have elapsed at room temperature, the plasmaogen content in a predetermined mass is 10% or more of the plasmaogen content in the said predetermined mass immediately after freeze-drying.

[0023] (17) A processed sea squirt product obtained by subjecting maboya or other sea squirts excluding the capsule to freeze-drying, and after the freeze-drying, storing at room temperature to 35°C for 2 months or more, and then using the contained plasmaogen. Or, a method of using the plasmaogen contained in a processed sea squirt product obtained by subjecting maboya or other sea squirts excluding the capsule to freeze-drying, and after the freeze-drying, storing the obtained processed sea squirt product at room temperature to 35°C for 2 months or more.

[0024] (18) A processed sea squirt product obtained by subjecting maboya or other sea squirts excluding the capsule to freeze-drying, and after the freeze-drying, storing at room temperature to 35°C for 4 months or more, and then using the contained plasmaogen. Or, a method of using the plasmaogen contained in a processed sea squirt product obtained by subjecting maboya or other sea squirts excluding the capsule to freeze-drying, and after the freeze-drying, storing the obtained processed sea squirt product at room temperature to 35°C for 4 months or more.

Advantages of the Invention

[0025] According to the manufacturing method of the present invention, it is possible to manufacture a processed sea squirt product with good stability over time of the contained plasmalogen. The processed sea squirt product of the present invention has improved stability over time of the contained plasmalogen. Further, according to the processed sea squirt product for utilizing the plasmalogen contained in the present invention, the contained plasmalogen can be effectively utilized even after being stored at room temperature to 35°C for a predetermined period or longer.

Brief Description of the Drawings

[0026]

Figure 1

Figure 2

Modes for Carrying Out the Invention

[0027] Embodiments of the present invention will be described.

[0028] (1) The manufacturing method of the present invention is a method for manufacturing a processed sea squirt product with good stability over time of the contained plasmalogen (that is, for example, it is difficult for plasmalogen decomposition or leakage to occur), It is to perform freeze-drying on all or part of Halocynthia roretzi excluding the tunic or other sea squirts (for example, the flesh of Halocynthia roretzi or other sea squirts, or the edible part of Halocynthia roretzi or other sea squirts).

[0029] As the step of performing freeze-drying, it has a step of pre-freezing all or part of Halocynthia roretzi excluding the tunic or other sea squirts, and a step of subjecting the pre-frozen Halocynthia roretzi or other sea squirts to sublimation drying under reduced pressure. Further, for example, it can have a step of drying so that there is no residual moisture in Halocynthia roretzi or other sea squirts.

[0030] In addition, substantially no substances other than water may be added to the maboya or other ascidians. Further, extraction with an organic solvent may not be performed.

[0031] (2) The processed ascidian of the present invention is a processed product of all or part of the maboya or other ascidians excluding the tunic, and the stability over time of the plasmalogen contained therein is improved by freeze-drying. Further, the processed ascidian for utilizing the plasmalogen contained in the present invention is a processed ascidian obtained by subjecting the maboya or other ascidians excluding the tunic to freeze-drying, and is stored at room temperature to 35 ° C for a predetermined period or longer after the freeze-drying. In any case, the step of performing freeze-drying can be the same as the step in the production method of the present invention. In addition, substantially no substances other than water may be added to the maboya or other ascidians. Further, extraction with an organic solvent may not be performed.

[0032] (3) The processed ascidian obtained by the production method of the present invention and the plasmalogen contained in the processed ascidian of the present invention are 1-alkenyl-2-acyl-sn-glycero-3-phosphate homologs having a vinyl ether bond at the sn-1(α) position of the glycerol molecule, and are alkenyl ether type phospholipids generally represented by the following formula [1].

[0033]

Chemical formula

[0034] In formula [1], R 1 is generally an alkyl group having 1 to 20 carbon atoms (for example, dodecyl group, tetradecyl group, hexadecyl group, octadecyl group, icosanyl group), and R 2 is generally an aliphatic hydrocarbon group corresponding to a residue of a polyunsaturated fatty acid (including DHA, EPA, arachidonic acid, etc.) or a monounsaturated fatty acid such as oleic acid (for example, R 2Examples of the CO group include octadecadienoyl group, octadecatrienoyl group, eicosatetraenoyl group, eicosapentaenoyl group, docosatetraenoyl group, docosapentaenoyl group, and docosahexaenoyl group. Preferably, it is an eicosapentaenoyl group or a docosahexaenoyl group. ), X is, for example, ethanolamine (the following formula [2]), choline (the following formula [3]), serine, inositol, or glycerol (preferably ethanolamine or choline).

[0035]

Chemical formula

[0036]

Chemical formula

[0037] (4) The production of processed sea squirt can be carried out, for example, as follows.

[0038] Maboya without the capsule or other sea squirt without the capsule, with all or part (if frozen, preferably thawed naturally as much as possible) other than the capsule, is treated (blanched) with hot water (for example, hot water at 90 - 95 °C). Preferably, it is treated by immersion in hot water (for example, while stirring).

[0039] After treatment for a predetermined time (for example, 10 minutes), the sea squirt without the capsule is pre-frozen and then freeze-dried under reduced pressure in a drying chamber, and further dried so that no moisture remains, whereby a freeze-dried processed sea squirt can be produced.

[0040] The obtained processed sea squirt can be used after undergoing coarse grinding, fine grinding, sieving, etc. as necessary.

[0041] (5) The processed sea squirt product obtained by the production method of the present invention, the processed sea squirt product of the present invention, or the processed sea squirt product for using the plasmalogen contained in the present invention can be provided for human consumption in the form of, for example, powder, tablets, hard capsules or other forms, but can also be used for other purposes. The oral dosage of the processed sea squirt product obtained by the production method of the present invention or the processed sea squirt product of the present invention for humans is preferably about 0.1 mg to 1000 mg / day in terms of the content of plasmalogen.

Examples

[0042] 1. Example

[0043] (1) Frozen sea squirt flesh (Halocynthia roretzi excluding the capsule) (952 kg) was thawed naturally at room temperature and then placed in a refrigerator for further thawing before being completely thawed.

[0044] When heating water with a weight 2.5 times that of the sea squirt flesh thawed in the refrigerator to 90 - 95°C in a tank, the thawed sea squirt flesh was put into the water at a water temperature of about 70°C.

[0045] Thereafter, heating was continued, and the sea squirt flesh was stirred at a water temperature of 90 - 95°C for 10 minutes to process (blanch) the sea squirt flesh. Then, the sea squirt flesh was discharged from the tank together with the hot water, and the sea squirt flesh was taken out.

[0046] The taken-out sea squirt flesh (343.8 kg) was packed into a freeze-drying vat and pre-frozen to -40°C over 12 hours or more. The drying chamber containing the pre-frozen sea squirt flesh in the freeze dryer was depressurized to 4 - 8 Pa and sublimation-dried over 65 hours or more, and further dried so that no moisture remained, thereby obtaining a freeze-dried processed sea squirt product (113.8 kg) at a temperature of 60°C.

[0047] The resulting sea squirt processed product was coarsely crushed (at about 1700 rpm) using a crusher with a 8φ (8 mm diameter) screen, then finely crushed (at 8000 rpm) using a fine crusher with a 1.5φ (1.5 mm diameter) screen, and sieved through a 32 mesh to obtain a finely crushed sea squirt processed product (113.1 kg). If necessary, foreign matter may be removed using a foreign matter removal device that utilizes magnetic force, etc.

[0048] (2) The plasmalogen content in 100 g of the finely crushed sea squirt processed product obtained was measured and found to be 1129 mg. The plasmalogen content was measured using the HPLC-ESI-MS / MS method. The same applies to the following examples.

[0049] (a) The finely ground sea squirt product was stored in a 35°C environment (without humidity control), and the plasmalogen content in 100 g of the finely ground sea squirt product was measured after 2 months, 4 months, and 6 months. The results were 832 mg (73.7%) after 2 months, 632 mg (56.0%), and 6 months, respectively. No abnormalities were observed in the appearance, powder properties, or odor. The figures in parentheses are the ratios to the plasmalogen content of 1129 mg in 100 g before storage.

[0050] (b) The finely ground sea squirt product was stored at room temperature, and after six months the plasmalogen content in 100 g of the finely ground sea squirt product was measured and found to be 788 mg (69.8%). No abnormalities were found in the appearance, powder properties, or odor. The figures in parentheses indicate the ratio to the plasmalogen content of 1129 mg in 100 g before storage.

[0051] The change over time in the plasmalogen content in 100 g of sea squirt product is shown in Figure 1, with the ordinate representing the percentage of the content before storage and the abscissa representing the months since storage.

[0052] The plasmalogen content in a given mass of sea squirt processed product decreases at a greater rate over time the higher the storage temperature (e.g., at or near room temperature, or at any other temperature).

[0053] From the measurement results and Figure 1, it can be said that the content of plasmalogen in the fine powder of the processed sea squirt product of a predetermined mass after freeze-drying is in the following ratio according to the number of months elapsed with respect to the plasmalogen content immediately after freeze-drying.

[0054] When 2 months have elapsed: In storage in an environment of 35°C (without humidity control), it is 5 - 74% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, or 70% or more), and in storage at room temperature, it can be said to be 5 - 90% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, or 85% or more).

[0055] When 4 months have elapsed: In storage in an environment of 35°C (without humidity control), it is 5 - 56% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, or 50% or more), and in storage at room temperature, it can be said to be 5 - 80% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, or 75% or more).

[0056] When 6 months have elapsed: In storage in an environment of 35°C (without humidity control), it is 5 - 59% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, or 55% or more), and in storage at room temperature, it can be said to be 5 - 70% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, or 65% or more).

[0057] 2. Comparative Example

[0058] (1) According to the method described in Example 1 of JP-A-2019-162042, "Weighed 50.8 g of freeze-dried powder of the edible part of the sea squirt, charged 66 mL of hexane, 121 mL of ethanol, and 11 mL of water, mixed them, and extracted at room temperature for 30 minutes while stirring. Then, filtered through qualitative filter paper (type: No. 2) to separate the extract and the residue. To the residue, 66 mL of hexane, 121 mL of ethanol, and 11 mL of water were charged again, mixed, and extracted at room temperature for 30 minutes while stirring. Then, filtered through qualitative filter paper (type: No. 2) to separate the extract and the residue. The two portions of the extract were combined and the solvent was removed by vacuum drying to obtain a sea squirt extract", 0.9446 g of sea squirt extract powder was obtained.

[0059] (2) When the content of plasmalogen in the obtained sea squirt extract powder was measured, it was 5540 mg per 100 g of the sea squirt extract powder.

[0060] (a) The sea squirt extract powder was stored in an environment at 40°C. When the content of plasmalogen in the same sea squirt extract powder was measured at 10 days, 20 days, 1 month, 2 months, and 3 months after the elapse of time, it was 2770 mg (50%), 831 mg (15%), 0 mg (0%), 0 mg (0%), and 0 mg (0%) per 100 g of the sea squirt extract powder, respectively. The figures in parentheses are the ratios to the content of plasmalogen of 5540 mg in 100 g before storage.

[0061] (b) The sea squirt extract powder was stored in an environment at 25°C. When the content of plasmalogen in the same sea squirt extract powder was measured at 10 days, 20 days, 1 month, 2 months, and 3 months after the elapse of time, it was 5097 mg (92%), 3712 mg (67%), 2936 mg (53%), 0 mg (0%), and 0 mg (0%) per 100 g of the sea squirt extract powder, respectively. The figures in parentheses are the ratios to the content of plasmalogen of 5540 mg in 100 g before storage.

[0062] The change over time in the content of plasmalogen with respect to 100 g of the Hoya extract powder is shown in Fig. 2, with the percentage with respect to the content before storage on the vertical axis and the elapsed months on the horizontal axis.

Claims

1. A method for producing a sea squirt processed product having good stability over time of the plasmalogen contained therein, A method for producing processed sea squirt products, comprising freeze-drying Halocynthia roretzi or other sea squirts, excluding the tunic.

2. 2. The method of claim 1, further comprising treating the tunicated Halocynthia or other sea squirt with boiling water prior to freeze-drying.

3. 2. The method according to claim 1, wherein substantially no substances other than water are added to said Halocynthia or other sea squirts.

4. The plasmalogen content in a predetermined mass of the sea squirt processed product after freeze-drying is 50% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 25 ° C. The manufacturing method according to any one of claims 1 to 3.

5. The plasmalogen content in a predetermined mass of the sea squirt processed product after freeze-drying is 40% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 35 ° C. The manufacturing method according to any one of claims 1 to 3.

6. The plasmalogen content in a predetermined mass of the sea squirt processed product after freeze-drying is 40% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 6 months in an environment at 25 ° C. The manufacturing method according to any one of claims 1 to 3.

7. The plasmalogen content in a predetermined mass of the sea squirt processed product after freeze-drying is 30% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 6 months in an environment of 35 ° C. The manufacturing method according to any one of claims 1 to 3.

8. A processed product of sea squirt, excluding the tunic, or other sea squirt, characterized in that the stability of the plasmalogen contained therein over time is improved by freeze-drying.

9. 9. The sea squirt processed product according to claim 8, which contains substantially no substances other than water other than the Halocynthia or other sea squirts.

10. The sea squirt processed product according to claim 8 or 9, wherein the plasmalogen content in a predetermined mass after freeze-drying is 30% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 25 ° C.

11. The sea squirt processed product according to claim 8 or 9, wherein the plasmalogen content in a predetermined mass after freeze-drying is 20% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 35 ° C.

12. The sea squirt processed product according to claim 8 or 9, wherein the plasmalogen content in a predetermined mass after freeze-drying is 20% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 6 months in an environment at 25 ° C.

13. The sea squirt processed product according to claim 8 or 9, wherein the plasmalogen content in a predetermined mass after freeze-drying is 10% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 6 months in an environment of 35 ° C.

14. The sea squirt processed product according to claim 8 or 9, wherein after freeze-drying, the plasmalogen content in a predetermined mass is 50% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying at room temperature for more than two months.

15. The sea squirt processed product according to claim 8 or 9, wherein after freeze-drying, the plasmalogen content in a predetermined mass is 30% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying at room temperature for more than two months.

16. The sea squirt processed product according to claim 8 or 9, wherein after freeze-drying, the plasmalogen content in a predetermined mass is 10% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying at room temperature for more than two months.

17. A sea squirt processed product obtained by freeze-drying a sea squirt or other sea squirt excluding the tunic, which is stored at room temperature to 35°C for more than two months after the freeze-drying and then used to utilize the plasmalogen contained therein.

18. A sea squirt processed product obtained by freeze-drying a sea squirt or other sea squirt excluding the tunic, which is stored at room temperature to 35°C for 4 months or more after the freeze-drying, and which is intended to utilize the plasmalogen contained therein.

Images