Method of producing processed ascidian, and processed ascidian

Abstract

To provide: a method of producing a processed ascidian having good temporal stability of contained plasmalogen; and a processed ascidian where the temporal stability of contained plasmalogen is improved.SOLUTION: The invention provides: a method of producing a processed ascidian having good temporal stability of contained plasmalogen by freeze-drying Halocynthia roretzi or another ascidian without encystment; and a processed product of Halocynthia roretzi or another ascidian without encystment, where the temporal stability of contained plasmalogen is improved by freeze drying.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a method for manufacturing processed products of mabooya or other sea squirts, and to processed sea squirt products.

Background Art

[0002] Japanese Patent Application Laid-Open No. 2016-210696 discloses a learning and memory ability enhancer for use in healthy mammals, which contains plasmalogen, and more than 90% of the plasmalogen is ethanolamine plasmalogen and choline plasmalogen. The plasmalogen is extracted from avian tissues, and preferably, the mass ratio of (ethanolamine plasmalogen: choline plasmalogen) contained in the plasmalogen is (1:5) to (5:1).

[0003] Japanese Patent Application Laid-Open No. 2019-162042 discloses an invention related to a plasmalogen-type phospholipid-rich extract that is an extract from seafood or aquatic animals, contains plasmalogen-type phospholipids with high purity, and has a reduced arsenic content, and a method for manufacturing the same.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0005] An object of the present invention is to provide a method for manufacturing a processed sea squirt product having good temporal stability of the contained plasmalogen, and a processed sea squirt product having improved temporal stability of the contained plasmalogen.

Means for Solving the Problems

[0006] The method for manufacturing a processed sea squirt of the present invention and the processed sea squirt can be represented as follows.

[0007] (1) A method for manufacturing a processed sea squirt having good stability over time of the contained plasmologen, characterized by subjecting maboya or other sea squirts excluding the tunic to freeze-drying.

[0008] (2) The manufacturing method according to (1) above, wherein the maboya or other sea squirts excluding the tunic are treated with hot water before the freeze-drying.

[0009] (3) The manufacturing method according to (1) or (2) above, wherein substantially no substance other than water is added to the maboya or other sea squirts.

[0010] (4) The manufacturing method according to any one of (1) to (3) above, wherein the plasmologen content in a predetermined mass of the processed sea squirt after freeze-drying is 50% or more of the plasmologen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 25°C.

[0011] (5) The manufacturing method according to any one of (1) to (3) above, wherein the plasmologen content in a predetermined mass of the processed sea squirt after freeze-drying is 40% or more of the plasmologen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 35°C.

[0012] (6) The manufacturing method according to any one of (1) to (3) above, wherein the plasmologen content in a predetermined mass of the processed sea squirt after freeze-drying is 40% or more of the plasmologen content in the predetermined mass immediately after freeze-drying after 6 months in an environment of 25°C.

[0013] (7) The method of manufacturing according to any one of (1) to (3) above, wherein the content of plasmalogen in a predetermined mass of the processed sea squirt product after freeze-drying is 30% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 6 months have elapsed in an environment of 35°C.

[0014] (8) A processed product of mabo sea squirt or other sea squirts excluding the capsule, characterized in that the stability over time of the plasmalogen contained therein is improved by freeze-drying.

[0015] (9) The processed sea squirt product according to (8) above, which substantially does not contain substances other than water in addition to the said mabo sea squirt or other sea squirts.

[0016] (10) The processed sea squirt product according to (8) or (9) above, wherein the content of plasmalogen in a predetermined mass after freeze-drying is 30% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 2 months have elapsed in an environment of 25°C.

[0017] (11) The processed sea squirt product according to (8) or (9) above, wherein the content of plasmalogen in a predetermined mass after freeze-drying is 20% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 2 months have elapsed in an environment of 35°C.

[0018] (12) The processed sea squirt product according to (8) or (9) above, wherein the content of plasmalogen in a predetermined mass after freeze-drying is 20% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 6 months have elapsed in an environment of 25°C.

[0019] (13) The processed sea squirt product according to (8) or (9) above, wherein the content of plasmalogen in a predetermined mass after freeze-drying is 10% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying after 6 months have elapsed in an environment of 35°C.

[0020] (14) The processed jellyfish product according to the above (8) or (9), wherein after freeze-drying, a period of two months or more has elapsed at room temperature, and the content of plasmalogen in a predetermined mass is 50% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying.

[0021] (15) The processed jellyfish product according to the above (8) or (9), wherein after freeze-drying, a period of two months or more has elapsed at room temperature, and the content of plasmalogen in a predetermined mass is 30% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying.

[0022] (16) The processed jellyfish product according to the above (8) or (9), wherein after freeze-drying, a period of two months or more has elapsed at room temperature, and the content of plasmalogen in a predetermined mass is 10% or more of the content of plasmalogen in the said predetermined mass immediately after freeze-drying.

[0023] (17) A processed jellyfish product obtained by subjecting maboya or other jellyfish excluding the capsule to freeze-drying, and after the freeze-drying, storing at room temperature to 35°C for two months or more, and then using the plasmalogen contained therein. Or, a method of using the plasmalogen contained in a processed jellyfish product obtained by subjecting maboya or other jellyfish excluding the capsule to freeze-drying, and after the freeze-drying, storing the obtained processed jellyfish product at room temperature to 35°C for two months or more.

[0024] (18) A processed jellyfish product obtained by subjecting maboya or other jellyfish excluding the capsule to freeze-drying, and after the freeze-drying, storing at room temperature to 35°C for four months or more, and then using the plasmalogen contained therein. Or, a method of using the plasmalogen contained in a processed jellyfish product obtained by subjecting maboya or other jellyfish excluding the capsule to freeze-drying, and after the freeze-drying, storing the obtained processed jellyfish product at room temperature to 35°C for four months or more.

Advantages of the Invention

[0025] According to the manufacturing method of the present invention, it is possible to manufacture a processed ascidian product having good stability over time of the contained plasmalogen. The processed ascidian product of the present invention has improved stability over time of the contained plasmalogen. Further, according to the processed ascidian product for using the contained plasmalogen of the present invention, even after being stored at room temperature to 35°C for a predetermined period or longer, the contained plasmalogen can be effectively utilized.

Brief Description of the Drawings

[0026]

Figure 1

Figure 2

Embodiments for Carrying Out the Invention

[0027] Embodiments of the present invention will be described.

[0028] (1) The manufacturing method of the present invention is a method for manufacturing a processed ascidian product having good stability over time of the contained plasmalogen (that is, for example, it is difficult for plasmalogen decomposition or leakage to occur), It is to perform freeze-drying on all or part of Halocynthia roretzi excluding the tunic or other ascidians (for example, the naked Halocynthia roretzi or other ascidians, or the edible parts of Halocynthia roretzi or other ascidians).

[0029] As the step of performing freeze-drying, it has a step of pre-freezing all or part of Halocynthia roretzi excluding the tunic or other ascidians, and a step of subjecting the pre-frozen Halocynthia roretzi or other ascidians to sublimation drying under reduced pressure. Further, for example, it can have a step of drying so that there is no residual moisture in Halocynthia roretzi or other ascidians.

[0030] Note that, for the maboya or other ascidians, it is possible to substantially add no substances other than water. Further, it is possible not to perform extraction with an organic solvent.

[0031] (2) The processed ascidian of the present invention is a processed product of all or part of the maboya or other ascidians excluding the tunic, and the stability over time of the plasmalogen contained therein is improved by freeze-drying. Further, the processed ascidian for using the plasmalogen contained in the present invention is a processed ascidian obtained by subjecting the maboya or other ascidians excluding the tunic to freeze-drying, and is stored at room temperature to 35°C for a predetermined period or longer after the freeze-drying. In any case, the step of performing freeze-drying can be the same as the step in the production method of the present invention. Note that, for the maboya or other ascidians, it is possible to substantially add no substances other than water. Further, it is possible not to perform extraction with an organic solvent.

[0032] (3) The processed ascidian obtained by the production method of the present invention and the plasmalogen contained in the processed ascidian of the present invention are 1-alkenyl-2-acyl-sn-glycero-3-phosphate homologs having a vinyl ether bond at the sn-1(α) position of the glycerol molecule, and are alkenyl ether type phospholipids generally represented by the following formula [1].

[0033] [Chemical formula] ....[1]

[0034] In formula [1], R 1 is generally an alkyl group having 1 to 20 carbon atoms (for example, dodecyl group, tetradecyl group, hexadecyl group, octadecyl group, icosanyl group), and R 2 is generally an aliphatic hydrocarbon group corresponding to a residue of a polyunsaturated fatty acid (including DHA, EPA, arachidonic acid, etc.) or a monounsaturated fatty acid such as oleic acid (for example, R 2Examples of the CO group include an octadecadienoyl group, an octadecatrienoyl group, an eicosatetraenoyl group, an eicosapentaenoyl group, a docosatetraenoyl group, a docosapentaenoyl group, and a docosahexaenoyl group. Preferably, it is an eicosapentaenoyl group or a docosahexaenoyl group. ), X is, for example, ethanolamine (the following formula [2]), choline (the following formula [3]), serine, inositol, or glycerol (preferably ethanolamine or choline).

[0035]

Chemical formula

[0036]

Chemical formula

[0037] (4) The production of the processed sea squirt can be carried out, for example, as follows.

[0038] The maboya without the capsule or other sea squirt without the capsule, with all or part (if frozen, preferably thawed naturally as much as possible) other than the capsule, is treated (blanched) with hot water (for example, hot water at 90 - 95 °C). Preferably, it is treated by immersion in hot water (for example, while stirring).

[0039] After treatment for a predetermined time (for example, 10 minutes), the sea squirt without the capsule is pre-frozen and then freeze-dried under reduced pressure in a drying chamber, and further dried so that no moisture remains, whereby a freeze-dried processed sea squirt can be produced.

[0040] The obtained processed sea squirt can be used after being subjected to coarse grinding, fine grinding, sieving, etc. as necessary.

[0041] (5) The processed sea squirt product obtained by the production method of the present invention, the processed sea squirt product of the present invention, or the processed sea squirt product for using the plasmalogen contained in the present invention can be provided for human consumption, for example, in the form of powder, tablets, hard capsules or other forms, but can also be used for other purposes. The oral dosage of the processed sea squirt product obtained by the production method of the present invention or the processed sea squirt product of the present invention for humans is preferably about 0.1 mg to 1000 mg / day in terms of the content of plasmalogen.

Example

[0042] 1. Example

[0043] (1) Frozen sea squirt flesh (Halocynthia roretzi without the capsule) (952 kg) was thawed naturally at room temperature and then placed in a refrigerator for further thawing before being completely thawed.

[0044] When heating water with a weight 2.5 times that of the sea squirt flesh thawed in the refrigerator to 90 - 95°C in a tank, the thawed sea squirt flesh was put into the water at a water temperature of about 70°C.

[0045] Thereafter, heating was continued, and the sea squirt flesh was stirred at a water temperature of 90 - 95°C for 10 minutes to process (blanch) the sea squirt flesh. Then, the sea squirt flesh was discharged from the tank together with the hot water, and the sea squirt flesh was taken out.

[0046] The taken-out sea squirt flesh (343.8 kg) was packed into a freeze-drying vat and pre-frozen at -40°C over 12 hours or more. The drying chamber containing the pre-frozen sea squirt flesh in the freeze dryer was depressurized to 4 - 8 Pa and sublimation dried over 65 hours or more, and further dried so that no residual moisture remained, thereby obtaining a freeze-dried processed sea squirt product (113.8 kg) at a temperature of 60°C.

[0047] The obtained processed sea squirt was roughly pulverized (at approximately 1700 rpm) using a pulverizer with an 8φ (8 mm diameter) screen, and then finely pulverized (at 8000 rpm) using a fine pulverizer with a 1.5φ (1.5 mm diameter) screen. After that, it was sieved through a 32-mesh sieve to obtain a finely pulverized product (113.1 kg) of the processed sea squirt. Foreign matter removal may be performed using a foreign matter removal device that utilizes magnetism or the like as necessary.

[0048] (2) When the content of plasmalogen in 100 g of the finely pulverized product of the obtained processed sea squirt was measured, it was 1129 mg. The measurement of the plasmalogen content was performed by the HPLC-ESI-MS / MS method. The same applies to the following examples.

[0049] (a) The finely pulverized product of the processed sea squirt was stored in an environment at 35°C (without humidity control). When the content of plasmalogen in 100 g of the same finely pulverized product of the processed sea squirt was measured at 2 months, 4 months, and 6 months after the passage of time, it was 832 mg (73.7%), 632 mg (56.0%), and 663 mg (58.7%) respectively. No abnormalities were observed in the appearance, powder properties, and odor. The figures in parentheses are the ratios to the content of plasmalogen in 100 g before storage, which was 1129 mg.

[0050] (b) The finely pulverized product of the processed sea squirt was stored at room temperature. When the content of plasmalogen in 100 g of the same finely pulverized product of the processed sea squirt was measured at 6 months after the passage of time, it was 788 mg (69.8%). No abnormalities were observed in the appearance, powder properties, and odor. The figure in parentheses is the ratio to the content of plasmalogen in 100 g before storage, which was 1129 mg.

[0051] The change over time in the content of plasmalogen in 100 g of the processed sea squirt is shown in Figure 1, with the percentage relative to the content before storage on the vertical axis and the number of months elapsed on the horizontal axis.

[0052] The higher the storage temperature (for example, at temperatures around room temperature or at other temperatures), the higher the rate of decrease over time in the plasmalogen content in a predetermined mass of the processed sea squirt.

[0053] From the measurement results and FIG. 1, it can be said that the content of plasmalogen in the fine powder of the processed sea squirt product of a predetermined mass after freeze-drying is in the following ratio according to the elapsed months with respect to the plasmalogen content immediately after freeze-drying.

[0054] When 2 months have elapsed: In storage in an environment of 35°C (without humidity control), it is 5 - 74% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, or 70% or more), It can be said that in storage at room temperature, it is 5 - 90% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, or 85% or more).

[0055] When 4 months have elapsed: In storage in an environment of 35°C (without humidity control), it is 5 - 56% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, or 50% or more), It can be said that in storage at room temperature, it is 5 - 80% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, or 75% or more).

[0056] When 6 months have elapsed: In storage in an environment of 35°C (without humidity control), it is 5 - 59% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, or 55% or more), It can be said that in storage at room temperature, it is 5 - 70% (for example, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, or 65% or more).

[0057] 2. Comparative Example

[0058] (1) According to the method described in Example 1 of JP-A-2019-162042, "Weighed 50.8 g of freeze-dried powder of edible part of jellyfish, charged 66 mL of hexane, 121 mL of ethanol, and 11 mL of water, mixed them, extracted at room temperature for 30 minutes while stirring, then filtered through qualitative filter paper (type: No. 2) to separate the extract and the residue. To the residue, 66 mL of hexane, 121 mL of ethanol, and 11 mL of water were charged again, mixed, extracted at room temperature for 30 minutes while stirring, and then filtered through qualitative filter paper (type: No. 2) to separate the extract and the residue. The two portions of the extract were combined and the solvent was removed by vacuum drying to obtain a jellyfish extract.", 0.9446 g of jellyfish extract powder was obtained.

[0059] (2) When the content of plasmalogen in the obtained jellyfish extract powder was measured, it was 5540 mg per 100 g of the jellyfish extract powder.

[0060] (a) The jellyfish extract powder was stored in an environment at 40 °C. When the content of plasmalogen in the same jellyfish extract powder was measured at 10 days, 20 days, 1 month, 2 months, and 3 months after the lapse of time, they were 2770 mg (50%), 831 mg (15%), 0 mg (0%), 0 mg (0%), and 0 mg (0%) per 100 g of the jellyfish extract powder, respectively. The figures in parentheses are the ratios to the content of plasmalogen of 5540 mg in 100 g before storage.

[0061] (b) The jellyfish extract powder was stored in an environment at 25 °C. When the content of plasmalogen in the same jellyfish extract powder was measured at 10 days, 20 days, 1 month, 2 months, and 3 months after the lapse of time, they were 5097 mg (92%), 3712 mg (67%), 2936 mg (53%), 0 mg (0%), and 0 mg (0%) per 100 g of the jellyfish extract powder, respectively. The figures in parentheses are the ratios to the content of plasmalogen of 5540 mg in 100 g before storage.

[0062] The change over time in the content of plasmalogen with respect to 100 g of ascidian extract powder is shown in Fig. 2, with the percentage with respect to the content before storage on the vertical axis and the number of months elapsed on the horizontal axis.

Claims

1. A method for producing a sea squirt processed product having good stability over time of the plasmalogen contained therein, A method for producing processed sea squirt products, comprising freeze-drying Halocynthia roretzi or other sea squirts, excluding the tunic.

2. 2. The method of claim 1, further comprising treating the tunicated Halocynthia or other sea squirt with boiling water prior to freeze-drying.

3. 2. The method according to claim 1, wherein substantially no substances other than water are added to said Halocynthia or other sea squirts.

4. The plasmalogen content in a predetermined mass of the sea squirt processed product after freeze-drying is 50% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 25 ° C. The manufacturing method according to any one of claims 1 to 3.

5. The plasmalogen content in a predetermined mass of the sea squirt processed product after freeze-drying is 40% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 35 ° C. The manufacturing method according to any one of claims 1 to 3.

6. The plasmalogen content in a predetermined mass of the sea squirt processed product after freeze-drying is 40% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 6 months in an environment at 25 ° C. The manufacturing method according to any one of claims 1 to 3.

7. The plasmalogen content in a predetermined mass of the sea squirt processed product after freeze-drying is 30% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 6 months in an environment of 35 ° C. The manufacturing method according to any one of claims 1 to 3.

8. A processed product of sea squirt, excluding the tunic, or other sea squirt, characterized in that the stability of the plasmalogen contained therein over time is improved by freeze-drying.

9. 9. The sea squirt processed product according to claim 8, which contains substantially no substances other than water other than the Halocynthia or other sea squirts.

10. The sea squirt processed product according to claim 8 or 9, wherein the plasmalogen content in a predetermined mass after freeze-drying is 30% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 25 ° C.

11. The sea squirt processed product according to claim 8 or 9, wherein the plasmalogen content in a predetermined mass after freeze-drying is 20% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 2 months in an environment of 35 ° C.

12. The sea squirt processed product according to claim 8 or 9, wherein the plasmalogen content in a predetermined mass after freeze-drying is 20% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 6 months in an environment at 25 ° C.

13. The sea squirt processed product according to claim 8 or 9, wherein the plasmalogen content in a predetermined mass after freeze-drying is 10% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying after 6 months in an environment of 35 ° C.

14. The sea squirt processed product according to claim 8 or 9, wherein after freeze-drying, the plasmalogen content in a predetermined mass is 50% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying at room temperature for more than two months.

15. The sea squirt processed product according to claim 8 or 9, wherein after freeze-drying, the plasmalogen content in a predetermined mass is 30% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying at room temperature for more than two months.

16. The sea squirt processed product according to claim 8 or 9, wherein after freeze-drying, the plasmalogen content in a predetermined mass is 10% or more of the plasmalogen content in the predetermined mass immediately after freeze-drying at room temperature for more than two months.

17. A sea squirt processed product obtained by freeze-drying a sea squirt or other sea squirt excluding the tunic, which is stored at room temperature to 35°C for more than two months after the freeze-drying and then used to utilize the plasmalogen contained therein.

18. A sea squirt processed product obtained by freeze-drying a sea squirt or other sea squirt excluding the tunic, which is stored at room temperature to 35°C for 4 months or more after the freeze-drying, and which is intended to utilize the plasmalogen contained therein.

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