Elastin composition containing needle-like substance

Abstract

To provide a low-cost elastin technique that can be used for liquid or the like, and is capable of infiltrating elastin into skin.SOLUTION: A needle-like substance containing elastin composition consists of elastin being an active ingredient for skin-whitening, and a needle-like substance, and exerts skin-whitening effect by an action of elastin. In the needle-like substance containing elastin composition of the present invention, elastin attaches to the needle-like substance, and elastin can reach a stratum basale on a lowermost part of epidermis by piercing the needle-like substance into skin. Further, elastin works on melanin production cells (melanocytes) in the stratum basale to cause tyrosinase inhibition, by which melanin production is suppressed, and skin-whitening effect can be exerted.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to an elastin composition containing needle-shaped material. [Background technology]

[0002] Elastin is a type of protein found in large amounts in tissues that require elasticity, such as skin, blood vessels, and ligaments, and it plays a role in providing elasticity to tissues. For this reason, elastin is widely used in health foods, medical products, and beauty products in the form of supplements and creams. On the other hand, elastin has a molecular weight of approximately 60,000 to 80,000 Da, and is relatively large in molecular size. Normally, it is said that the molecular weight of a drug required to penetrate human skin is 500 Da or less, and from this point of view, some kind of technical ingenuity is required to penetrate elastin into the skin. For these reasons, the inventors are developing microneedle technology that uses elastin as an active ingredient (Patent Document 1). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] JP 2023-16611 A Summary of the Invention [Problem to be solved by the invention]

[0004] The technology of Patent Document 1 is useful in that it can turn elastin itself into microneedles and efficiently achieve penetration into the skin. However, the creation of microneedles from elastin itself requires precise adjustments, which poses a problem in that the product itself is expensive.In addition, since the basic structure of the microneedle is that the entire microneedle is a solid, it is difficult to use it as a solution or cream. In light of the above circumstances, the present invention aims to provide elastin technology that is lower cost, can be used in liquids, etc., and can penetrate the skin. [Means for solving the problem]

[0005] After extensive research, the inventor came up with the idea of ​​a composition that mixes elastin with a needle-shaped substance, and completed the invention.

[0006] The present invention comprises the following configuration. [1] An elastin composition containing a needle-shaped substance, which comprises elastin, an active ingredient for whitening, and a needle-shaped substance, and exhibits a whitening effect through the action of elastin. [2] The elastin composition containing needle-shaped material described in [1], wherein the needle-shaped material has a length of 100 to 300 μm. [3] An elastin composition containing a needle-shaped material as described in [2], in which the needle-shaped material is composed primarily of silica gel or hydroxyapatite. [4] An elastin composition containing a needle-shaped material as described in [3], wherein the needle-shaped material is natural bone needles. [5] Furthermore, it contains a solvent as a component, The solvent is water, glycerin, propanediol, ethanol, butylene glycol, sorbitol, glyceryl triethylhexanoate, dipropylene glycol, propylene glycol, glyceryl glucoside, The needle-shaped material-containing elastin composition according to claim 1, selected from any one or more of these. [6] An elastin composition containing a needle-shaped substance as described in [5], further comprising an additive. [7] A needle-shaped substance-containing elastin composition described in any one of [1] to [6], which is used by applying to the skin. Effect of the Invention

[0007] The present invention makes it possible to provide elastin technology that is lower cost, can be used in liquids, etc., and can penetrate the skin. [Brief description of the drawings]

[0008] [Figure 1] A graph comparing photographs of the skin's appearance before and three months after the start of the study. [Diagram 2] A graph comparing the analysis results of skin condition before and three months after the start of the study. [Diagram 3] A graph comparing the analysis results of skin condition before and three months after the start of the study. [Figure 4] A diagram showing the analysis results of needle-shaped material (cancellous bone needles). DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0009] The needle-shaped substance-containing elastin composition of the present invention will now be described. The needle-shaped substance-containing elastin composition of the present invention is characterized by comprising elastin, which is an active ingredient for whitening, and a needle-shaped substance, and exhibiting a whitening effect through the action of elastin. In other words, elastin adheres to the needle-shaped substance, and the needle-shaped substance penetrates the skin, allowing elastin to reach the basal layer at the bottom of the epidermis. Elastin also acts on melanin-producing cells (melanocytes) in the basal layer, inhibiting tyrosinase, thereby suppressing melanin production and providing a whitening effect. Furthermore, the tyrosinase inhibitory activity of elastin has been clarified by a patent application filed by the present inventor (Patent Application No. 2023-099701).

[0010] The needle-shaped substance-containing elastin composition of the present invention is in the form of a liquid, cream, foundation, etc. when used, and can be used by applying it to the skin. That is, in one embodiment of the acicular material-containing elastin composition of the present invention, it can be configured as a non-solid composition containing elastin and a acicular material, such as a liquid, suspension, cream, powder, etc. In another embodiment of the acicular material-containing elastin composition of the present invention, it can be configured as a solid combining elastin and a acicular material, which can be dissolved or suspended using a solvent or the like as necessary and used as a non-solid composition such as a liquid, suspension, cream, powder, etc. The needle-shaped material-containing elastin composition of the present invention can be used by applying such a non-solid composition to the skin, and continued use of this composition can provide a whitening effect.

[0011] The elastin used in the present invention may be derived from animals or fish, preferably from food animals, and more preferably from pigs or cows.

[0012] Elastin can be obtained by known methods. For example, biological tissues such as aortic blood vessels obtained from mammals such as pigs, horses, cows, and sheep, or biological tissues such as avian arterial blood vessels and fish arterial bulbs, are used as elastin raw materials. These biological tissues are broken down into small pieces by homogenization or the like. Next, collagen and other substances are removed from the broken down samples using an alkaline solution or the like, and insoluble elastin can be obtained. The molecular weight of such elastin can be adjusted by performing membrane separation or column purification using gel filtration, and the average molecular weight of elastin can be adjusted to typically less than 300,000, preferably less than 100,000, and particularly preferably 20,000 to less than 30,000.

[0013] The elastin used in the present invention may be a decomposition product of elastin. The decomposition product of elastin may be obtained by a known method. For example, with respect to the insoluble elastin obtained above, hydrolysis treatment is performed using an acid or an alkali or the like, and separation work is performed on this treatment solution to obtain water-soluble elastin. In addition, during this separation work, membrane separation, column purification by gel filtration, etc. are performed to obtain a fraction of an elastin degradation product with a controlled molecular weight, and in that fraction, an elastin degradation product with a molecular weight of 100 to less than 2,000 can be obtained.

[0014] In the present invention, the needle-like substance plays a role of attaching elastin and piercing the epidermis of the skin to reach the basal layer at the bottom of the epidermis, so as to act on the melanin-producing cells in the basal layer. In addition, the needle-like substance needs to maintain a needle-like shape in a solvent and needs to be hardly soluble or insoluble in the solvent. The needle-like substance does not need to be particularly limited as long as it has such a role or property, and various substances can be used. The needle-like substance can typically be 100 to 1000 μm, preferably 100 to 800 μm, more preferably 100 to 600 μm, still more preferably 100 to 400 μm, particularly preferably 100 to 300 μm, and most preferably 200 to 300 μm. As a hardly soluble or insoluble material, the needle-like substance can be used with silica gel or hydroxyapatite as a main component. In addition, natural bone needles can be used as the needle-like substance with silica gel as a main component.

[0015] Various additives can be used in the needle-like substance-containing elastin composition of the present invention. Examples of such additives include a solubilizer (solvent), a stabilizer, an emulsifier, a fragrance, a preservative, a sunscreen agent, etc.

[0016] Examples of dissolving agents (solvents) include water, glycerin, propanediol, ethanol, butylene glycol, sorbitol, glyceryl triethylhexanoate, dipropylene glycol, propylene glycol, and glyceryl glucoside, and these can be used alone or in combination.

[0017] Examples of stabilizers include sodium ethylenediaminetetraacetate (Sodium EDTA), vitamin E (Tocopherol), vitamin C derivatives (Ascorbyl Palmitate), carbomer, hydroxypropyl cellulose, citric acid, gluconic acid, mannitol, panthenol, ceramides, nicotinamide, sodium citrate, dipotassium glycyrrhizate, hyaluronic acid, phenoxyethanol, benzyl alcohol, glycerin, tocopheryl acetate, honey, and lecithin, and these can be used alone or in combination.

[0018] Emulsifiers include, for example, Cetearyl Alcohol, Sorbitan Stearate, Polysorbate 20, Glyceryl Stearate, Polysorbate 60, Polysorbate 80, Stearic Acid, Steareth-20, Glyceryl Oleate, Lauryl Glucoside, Tetrasodium Glutamate Diacetate, Cyclopentasiloxane, Polyethylene Glycol, Hexyl Glyceryl Stearate, and Di(phytosteryl / octyldodecyl) Lauroyl Glutamic Acid. Di(Phytosteryl / Octyldodecyl)), Glyceryl Oleate, Polyglyceryl-10 Laurate, Isostearic Acid Sorbitan, Lauroyl Glutamic Acid Di(Phytosteryl / Behenyl) and Polyoxyethylene Hardened Castor Oil, which can be used alone or in combination.

[0019] Examples of fragrances include rose, lavender, sandalwood, bergamot, peach, vanilla, jasmine, lemon, green tea, musk, orange, apple, pink grapefruit, mint, berry, citrus, coconut, ginger, cedarwood, pine wood, and aroma oils (lavender oil, peppermint oil, rosemary oil, bergamot oil, jasmine oil, sandalwood oil, cedarwood oil, pine oil, rosewood oil, etc.), and these can be used alone or in combination.

[0020] Preservatives include, for example, phenoxyethanol, methylparaben, propylparaben, ethylparaben, Examples include butylparaben, isobutylparaben, phenoxymethylparaben, benzyl alcohol, chlorphenesin, citric acid, glycerin, phenoxyethyl hexylglycerin, isopropyl alcohol, sorbic acid, phenoxyethyl alcohol, phenoxyethyl butyrate, 2-phenoxyethanol, caprylyl glycol, polyhexamethylene biguanide, and dimethicone. These can be used alone or in combination.

[0021] Sunscreens include, for example, titanium dioxide, zinc oxide, octyl methoxycinnamate, avobenzone, octinoxate, methoxycrylene silyl trimethicone, terephthalylidene dicamphor sulfonic acid, hexyl salicylate, ethylhexyl methoxycinnamate, ethylhexyl triazone, benzalkonium chloride, methoxydibenzoylmethane (Mexoryl SX), dimethicone, homosalate, and octyl triazone. Triazone), Octocrylene, Dioctyl Adipate, Dibutyl Adipate, Methoxydibenzoylhexylhexylisothioate, Methyldibromo Glutaronitrile, Ethylhexyl Salicylate, etc., which can be used alone or in combination. EXAMPLES

[0022] The needle-shaped substance-containing elastin composition of the present invention will now be described in further detail with reference to examples. Furthermore, for each test subject, evaluation tests of the elastin composition containing needle-shaped substances were conducted in confidence after a full explanation was given to each subject and their consent was obtained.

[0023] <<Experimental Example 1, Verification of the effect of needle-shaped material-containing elastin composition on the skin>> 1. Experiments were conducted to determine the effect of continuously applying an elastin composition containing needle-shaped substances to the skin on blemishes and whitening. 2. Approximately 70 mg of elastin (Vital Resources Application Research Institute, Inc.) was mixed with about an earpick's worth of natural bone needles (approximately 1 mg), and then dissolved in sterile water until a suitable viscosity was achieved. This was prepared at the time of use and used as the test liquid. 3. Once a week, the test liquid was applied to the spots on the back of the right hand, mainly on the spots, and left to dry slightly.

[0024] 4. The appearance photograph results are shown in Figure 1. (1) Before the start of the test, there were several spots on the back of the right hand (a). (2) Three months after the start of the study, the spots on the back of the right hand had clearly faded and become lighter (b). The skin around the spots had also become relatively whiter (b). (3) These results confirmed that continued use of the test solution was effective in fading blemishes and whitening the skin.

[0025] 5. The results of skin analysis are shown in Figures 2 and 3. (1) Melanin, an indicator of whiteness, was 3 before the start of the study, but had improved to 4 about three months later. (2) In addition, the overall skin score before the start of the study was 61, but after approximately three months it had improved to 72, indicating that the overall skin index had improved. (3) These results also confirmed that continued use of the test solution improved melanin levels and had a positive effect on the entire skin.

[0026] <<Experimental example, needle-shaped substance analysis results>> 1. The results of analysis of the needle-shaped material used in Experimental Example 1 are shown in FIG. (1) The needle-like material used (cancellous bone needles) was composed of atoms of Si (silica gel), C (carbon), and O (oxygen). (2) The length of the needle-like substances ranged from 200 to 300 μm.

Claims

1. It consists of elastin, an active ingredient for whitening, and needle-shaped substances. An elastin composition containing an acicular substance, characterized in that it exerts a whitening effect by the action of elastin.

2. 2. The elastin composition containing needle-shaped material according to claim 1, wherein the needle-shaped material has a length of 100 to 300 μm.

3. The elastin composition containing needle-shaped material according to claim 2, wherein the needle-shaped material is composed primarily of silica gel.

4. The elastin composition containing needle-shaped material according to claim 3, wherein the needle-shaped material is a natural bone needle.

5. Further, the composition contains a solvent as a component. The solvent is water, glycerin, propanediol, ethanol, butylene glycol, sorbitol, glyceryl triethylhexanoate, dipropylene glycol, propylene glycol, glyceryl glucoside, The needle-shaped substance-containing elastin composition according to claim 1, selected from any one or more of the above.

6. The needle-shaped substance-containing elastin composition according to claim 5, further comprising an additive.

7. A needle-shaped material-containing elastin composition according to any one of claims 1 to 6, which is used by applying it to the skin.

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