Liposome-containing composition for cosmetics

Abstract

To provide a liposome-containing composition for cosmetics, which contains ceramides, allows an active ingredient to be encapsulated in liposomes at a high encapsulation rate, and contains fine liposomes.SOLUTION: A liposome-containing composition for cosmetics contains a liposome containing phospholipids, ceramides, polyoxyethylene phytosterol, and at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol, wherein when the content of polyoxyethylene phytosterol is defined as "c" and the total content of cholesterol and phytosterols other than polyoxyethylene phytosterol is defined as "d", the mass ratio d / c is 1.4 to 2.5.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present disclosure relates to a liposome-containing composition for cosmetics. [Background technology]

[0002] Liposomes are closed vesicles formed by a lipid bilayer containing lipids such as lecithin, and have an aqueous phase (internal aqueous phase) within the space of the closed vesicles. In addition, liposomes usually exist in a dispersed state in an aqueous solution (external aqueous phase) outside the closed vesicles.

[0003] Liposomes, also called lipid vesicles, are closed vesicles formed of a lipid bilayer membrane using lipids, and have an aqueous phase in the space of the closed vesicles. Various encapsulated components can be blended into liposomes, and various liposome-containing compositions for cosmetics containing liposomes encapsulating beauty ingredients, medicinal ingredients, etc. have been proposed.

[0004] For example, Patent Document 1 proposes a liposome dispersion liquid that is characterized by containing ceramides, phospholipids, a diester of an N-acylamino acid, and a polyalkyloxysterol ether. Patent Document 2 proposes a composition containing a bicellar structure, which contains (a) a phospholipid, (b) a ceramide, (c) at least one surfactant selected from the group consisting of polyoxyethylene sterol ether and polyoxyethylene stanol ether, (d) at least one dihydric alcohol selected from the group consisting of propylene glycol, dipropylene glycol, and 1,3-butylene glycol, and (e) water, in which the mass ratio (a) / (c) of component (a) to component (c) and the mass ratio (b) / {(a)+(c)} of component (b) to the total of components (a) and (c) are within predetermined ranges. Furthermore, Patent Document 3 proposes a liposome composition for cosmetics that contains a cosmetic ingredient in its internal aqueous phase and contains liposomes with an average particle size of 100 nm to 350 nm. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] JP 2016-160242 A [Patent Document 2] JP 2020-19764 A [Patent Document 3] JP 2022-149691 A Summary of the Invention [Problem to be solved by the invention]

[0006] When liposomes encapsulating cosmetic ingredients are used in cosmetics, there is a demand for liposomes that can encapsulate a larger amount of cosmetic ingredients. To encapsulate a water-soluble component in the aqueous phase, it is necessary to ensure the volume of the internal aqueous phase, but if the particle size is increased by increasing the volume of the internal aqueous phase, the turbidity of the preparation such as a cosmetic increases, the transparency deteriorates, and the appearance may be affected. In particular, liposomes containing ceramides, which contribute to improving the barrier function of the skin, tend to have a large particle size and an increase in turbidity.

[0007] The liposomes contained in the composition proposed in Patent Document 1 have the problem that they do not adequately retain the encapsulated components. The bicellar structure contained in the composition described in Patent Document 2 contains ceramide, but due to its structure, there is almost no room for including aqueous phase components, and there is room for improvement in the encapsulation rate of cosmetic ingredients. The liposomes contained in the composition proposed in Patent Document 3 have a fine particle size and can contain encapsulated components, but there is no focus on the inclusion of ceramide.

[0008] An object of one embodiment of the present disclosure is to provide a liposome-containing composition for cosmetics that contains ceramides, can contain an active ingredient encapsulated in liposomes at a high encapsulation rate, and contains fine liposomes. [Means for solving the problem]

[0009] <1> A liposome-containing composition for cosmetics, comprising liposomes containing a phospholipid, a ceramide, a polyoxyethylene phytosterol, and at least one of cholesterol and a phytosterol other than polyoxyethylene phytosterol, wherein the content of the polyoxyethylene phytosterol is c and the total content of the cholesterol and the phytosterol other than polyoxyethylene phytosterol is d, and the mass ratio of d / c is 1.4 to 2.5. <2> The liposome has an average particle size of 150 nm or less. <1> The liposome-containing cosmetic composition according to claim 1. <3> The ceramides are at least one selected from the group consisting of ceramide NDS, ceramide ADS, ceramide EODS, ceramide NS, ceramide AS, ceramide EOS, ceramide NP, ceramide AP, ceramide EOP, ceramide NH, ceramide AH, and ceramide EOH. <1> or <2> The liposome-containing cosmetic composition according to claim 1. <4> The polyoxyethylene phytosterol has an average added mole number of oxyethylene groups of 10 or more. <1> ~ <3> 10. The liposome-containing cosmetic composition according to claim 9 , <5> The content of the polyoxyethylene phytosterol relative to the total mass of the liposome-containing composition for cosmetics is 0.1% by mass or more. <1> ~ <4> 10. The liposome-containing cosmetic composition according to claim 9 , <6> the total content of phytosterols other than the cholesterol and polyoxyethylene phytosterol is 0.2% by mass to 0.5% by mass relative to the total mass of the liposome-containing composition for cosmetics; <1> ~ <5> 10. The liposome-containing cosmetic composition according to claim 9 , <7> The liposomes include liposomes having a single lamellar structure. <1> ~ <6> 10. The liposome-containing cosmetic composition according to claim 9 , <8> Cosmetics <1> ~ <7> 10. The liposome-containing cosmetic composition according to claim 9 , Effect of the Invention

[0010] According to one embodiment of the present disclosure, it is possible to provide a liposome-containing composition for cosmetics that contains ceramides, can contain an active ingredient encapsulated in liposomes at a high encapsulation rate, and contains fine liposomes. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0011] Hereinafter, the embodiments for carrying out the present disclosure will be described in detail. However, the present invention is not limited to the following embodiments. In the following embodiments, the components are not essential unless specifically stated otherwise. The same applies to the numerical values ​​and their ranges, and they do not limit the present disclosure.

[0012] In the present disclosure, a numerical range indicated using "~" includes the numerical values ​​before and after "~" as the minimum and maximum values, respectively. In the present disclosure, in which numerical ranges are described in stages, the upper or lower limit value described in one numerical range may be replaced with the upper or lower limit value of another numerical range described in stages. In addition, in the numerical ranges described in the present disclosure, the upper or lower limit of the numerical range may be replaced with values ​​shown in the examples. In the present disclosure, each component may contain multiple types of corresponding compounds. When multiple substances corresponding to each component are present in the composition, the content of each component means the total content of the multiple substances present in the composition, unless otherwise specified.

[0013] In the present disclosure, a "liposome" is a closed endoplasmic reticulum formed by a lipid bilayer membrane. A structure having a single lipid bilayer is called a single lamellar structure, and a structure having multiple lipid bilayers is called a multilamellar structure. In the present disclosure, a liposome having a single lamellar structure is sometimes also called a "unilamellar liposome." In this disclosure, "internal aqueous phase" refers to the solution present within closed endoplasmic reticulum. In this disclosure, "external aqueous phase" refers to the solution in which the liposomes are dispersed.

[0014] In this disclosure, "cholesterol" is a general term for sterols contained in animals, and "phytosterol" is a general term for sterols contained in plants.

[0015] In the present disclosure, the term "beauty ingredient" refers to an ingredient that acts on the body, such as the skin, and means an active ingredient used in cosmetics and the like. Moreover, the term "water-soluble" means that the solubility in 100 g of water at a liquid temperature of 22° C. and a pH of 7.0 is 0.1 g or more.

[0016] In the present disclosure, the term "average particle size" refers to the volume average particle size determined by dynamic light scattering. Examples of dynamic light scattering particle size measuring instruments include the Concentrated System Particle Size Analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.), Nanotrack UPA (manufactured by Nikkiso Co., Ltd.), and Dynamic Light Scattering Particle Size Distribution Measuring Instrument LB-550 (manufactured by Horiba, Ltd.). The average particle size is measured by diluting the liposome-containing composition for cosmetics with pure water to 10 times its mass, at room temperature (for example, 25° C.). The "pure water" used herein is pure water obtained using an ultrapure water production apparatus manufactured by Merck Ltd.

[0017] In the present disclosure, "the ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome-containing composition for cosmetics" is determined by observing the liposomes contained in the liposome-containing composition for cosmetics in an ice-embedded state using a transmission electron microscope (hereinafter referred to as TEM) and counting the total number of liposomes contained in the liposome-containing composition for cosmetics and the total number of liposomes having a single lamellar structure. More specifically, first, a TEM image of the liposome-containing cosmetic composition at a magnification of 50,000 times is obtained using a transmission electron microscope. The total number of liposomes present within a circle of radius 2 μm and the total number of liposomes having a single lamellar structure are counted at each of three arbitrary points on the TEM image to determine the "ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome-containing composition for cosmetic use." In the present disclosure, the "ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome-containing composition for cosmetics" is the average value of three points. In the present disclosure, the encapsulation rate of a cosmetic ingredient in a liposome can be evaluated by the "average particle size of the liposomes" and the "ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome-containing composition for cosmetics."

[0018] (Liposome-containing composition for cosmetics) The liposome-containing composition for cosmetics of the present disclosure is a liposome containing a phospholipid, a ceramide, a polyoxyethylene phytosterol, and at least one of cholesterol and a phytosterol other than polyoxyethylene phytosterol, and contains a liposome (hereinafter also referred to as a specific liposome) in which d / c is a mass ratio of 1.4 to 2.5, where c is the content of the polyoxyethylene phytosterol and d is the total content of the phytosterols other than the cholesterol and polyoxyethylene phytosterol.

[0019] The liposome-containing composition for cosmetics of the present disclosure (hereinafter also referred to as the composition of the present disclosure) is made into the specific liposome of the above-mentioned embodiment, and thereby can contain an aqueous active ingredient (beauty ingredient) at a high encapsulation rate, and the liposome for cosmetics has a fine particle size.

[0020] The reason why the above effect is achieved is considered to be as follows. The specific liposome contained in the liposome-containing composition for cosmetics of the present disclosure contains a phospholipid (hereinafter also referred to as component A), a ceramide (hereinafter also referred to as component B), a polyoxyethylene phytosterol (hereinafter also referred to as component C), and at least one phytosterol other than polyoxyethylene phytosterol (hereinafter also referred to as component D). The specific liposome contains polyoxyethylene phytosterol (ie, component C), which inhibits the lipid bilayer membranes from approaching each other, and component C contributes to the formation of the liposome. In addition, it was known that the incorporation of ceramides (i.e., component B) tends to increase the particle size of liposomes, but by setting the content ratio (d / c) of component C to at least one of phytosterols other than cholesterol and polyoxyethylene phytosterol (i.e., component D) to 1.4 to 2.5 and relatively increasing the content ratio of component D to component C, it is speculated that, although the reason is not clear, even when subjected to high-pressure emulsification conditions aimed at microparticulating the liposomes, an increase in particle size or the occurrence of undesirable bicellar structures is suppressed, the proportion of liposomes having a single lamellar structure is increased, component B is retained in the lipid bilayer membrane, which is the external phase, and a decrease in the encapsulation rate of the cosmetic ingredient in the aqueous phase is suppressed. It should be noted that the above-mentioned mechanism of action is merely speculation and does not limit the present disclosure in any way.

[0021] The specific liposome according to the present disclosure and each component contained in the composition of the present disclosure containing the specific liposome will be described below. In the specific liposome, the following phospholipids (component A), ceramides (component B), component C, and component D are contained in the lipid bilayer membrane that constitutes the specific liposome.

[0022] (A) Phospholipids (Component A) The particular liposome comprises an A component. There are no particular limitations on the phospholipid (component A) that may be used as long as it is a phospholipid that can be used in cosmetics, and an example of such a phospholipid is lecithin, which is a phospholipid derived from a natural product. Lecithin refers to phosphatidylcholine (hereinafter also referred to as PC) itself, or a mixture containing at least phosphatidylcholine. A mixture containing at least PC is generally a mixture that may contain, in addition to PC, phosphatidylserine, phosphatidylethanolamine, phosphatidylinositol, N-acylphosphatidylethanolamine, phosphatidylglycerol, phosphatidic acid, lysophosphatidylcholine, lysophosphatidic acid, sphingomyelin, sphingoethanolamine, and the like.

[0023] Examples of lecithin include natural lecithin obtained from plants such as soybean, rapeseed, sunflower, safflower, peanut, cottonseed, corn, rice, barley, and egg yolk, as well as derivatives of these (hereinafter also referred to as lecithin derivatives). Examples of lecithin derivatives include hydrogenated lecithin, and compounds in which polyethylene glycol, aminoglycans, etc. are introduced into the phospholipids in lecithin. Among the above, hydrogenated lecithin is particularly preferred from the viewpoint of oxidation stability.

[0024] From the viewpoint of the stability over time of the liposome-containing cosmetic composition, the content of phosphatidylcholine relative to the total mass of lecithin (hereinafter also referred to as PC content) is preferably 80% by mass or more, more preferably 85% by mass or more, and even more preferably 90% by mass or more. The PC content in the phospholipids can be measured by subjecting the phospholipid composition to a TLC-FID method or an HPLC method.

[0025] Commercially available lecithin includes COATSOME NC-21 (PC content 90% by mass or more) manufactured by NOF Corporation and NIKKOL (registered trademark) Lecithinol S-10E (PC content 75% by mass to 85% by mass or more) manufactured by Nikko Chemicals Co., Ltd., and from the viewpoint of PC content, COATSOME NC-21 is preferred.

[0026] From the viewpoints of lipid bilayer formation and stability over time, the content of lecithin relative to the total mass of the liposome-containing composition for cosmetics is preferably 0.3% by mass to 10% by mass, more preferably 0.4% by mass to 5% by mass, and even more preferably 0.5% by mass to 3% by mass.

[0027] (B) Ceramides (Component B) The specific liposome according to the present disclosure contains ceramides (component B). As the ceramides, human ceramides can be used, and pseudoceramides synthesized to mimic the structure of active ceramides can also be used. The ceramides can be appropriately selected from those contained in cosmetics. The ceramide is preferably at least one selected from the group consisting of ceramide NDS, ceramide ADS, ceramide EODS, ceramide NS, ceramide AS, ceramide EOS, ceramide NP, ceramide AP, ceramide EOP, ceramide NH, ceramide AH, and ceramide EOH, and among these, ceramide EOP, ceramide NP, and ceramide AP are preferred. The specific liposome may contain only one type of ceramide as the component (B), or may contain two or more types. The content of the (B) component in the specific liposome can be appropriately selected depending on the purpose. In general, the content of the (B) component can be 0.001% by mass to 1.0% by mass, preferably 0.01% by mass to 0.1% by mass, and most preferably 0.05% by mass to 0.09% by mass, based on the total mass of the specific liposome.

[0028] (C) Polyoxyethylene phytosterol (ingredient C) Polyoxyethylene phytosterols are (CH 2 CH 2 -O) n It is a phytosterol having an oxyethylene group represented by the formula: In this disclosure, phytosterols include hydrogenated phytosterols (phytostanols). Specific examples of phytosterols include sitosterol, stigmasterol, fucosterol, spinasterol, brassicasterol, and hydrogenated products thereof.

[0029] From the viewpoint of improving the encapsulation rate of a beauty ingredient, the polyoxyethylene phytosterol preferably has an average added mole number of oxyethylene groups of 5 or more, more preferably 10 or more, and even more preferably 20 or more. Here, by setting the average number of moles of oxyethylene groups added in the polyoxyethylene phytosterol to a preferred range of 10 or more, the liposome-containing composition for cosmetics can be The ratio of liposomes having a single lamellar structure can be increased, and the encapsulation rate of the cosmetic ingredient in the liposomes can be further improved. Note that the catalog value can be referred to for the average number of moles of oxyethylene groups added to the polyoxyethylene phytosterol.

[0030] Commercially available polyoxyethylene phytosterols include NIKKOL (registered trademark) BPS-5 (average number of moles of oxyethylene groups added: 5), NIKKOL (registered trademark) BPS-10 (average number of moles of oxyethylene groups added: 10), NIKKOL (registered trademark) BPS-20 (average number of moles of oxyethylene groups added: 20), and NIKKOL (registered trademark) BPS-30 (average number of moles of oxyethylene groups added: 30), NIKKOL (registered trademark) BPSH-25 (average number of moles of oxyethylene groups added: 25), all of which are manufactured by Nikko Chemicals Co., Ltd. Examples of such ethylene oxide adducts include EMALEX® PS-5 (average number of moles of oxyethylene groups added: 5), EMALEX® PS-10 (average number of moles of oxyethylene groups added: 10), EMALEX® PS-20 (average number of moles of oxyethylene groups added: 20), EMALEX® PS-30 (average number of moles of oxyethylene groups added: 30), and EMALEX® PS-25 (average number of moles of oxyethylene groups added: 25), all manufactured by Nippon Emulsion Co., Ltd.

[0031] The content of component C relative to the total mass of the specific liposome is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, and even more preferably 0.2% by mass or more. By ensuring that the content of component (C) is 0.05% by mass or more, the proportion of liposomes having a single lamellar structure in the liposome-containing cosmetic composition can be increased, and the encapsulation rate of the cosmetic ingredient in the liposomes can be further improved. Furthermore, the content of polyoxyethylene phytosterol relative to the total mass of the specific liposome is preferably 0.70% by mass or less, more preferably 0.50% by mass or less, and even more preferably 0.30% by mass or less. When the content of component (C) is 0.07% by mass or less, liposomes are easily formed and the formation of undesired disk particles or micelles can be suppressed. The content of component (C) is preferably in the range of 0.1% by mass to 0.5% by mass, and more preferably in the range of 0.2% by mass to 0.3% by mass. By having the content in the above range, it can be said that it is easier to obtain specific liposomes with a small particle size and a high proportion of liposomes having a single lamellar structure.

[0032] (D) Phytosterols other than cholesterol and polyoxyethylene phytosterols (i.e., component (D)) In this disclosure, cholesterol includes cholestanol and cholesterol derivatives. Examples of cholesterol include cholesterol, dihydrocholesterol, dehydrocholesterol, cholesteryl oleate, cholesteryl isostearate, cholesteryl hydroxystearate, and polyoxyethylene cholesteryl ether. Examples of component (D) include cholesterol, which is an animal sterol, as well as phytosterol, which is a plant sterol, and further include phytostanol, which is a hydrogenated product of plant sterol, as component (D). In component (D), phytosterols other than polyoxyethylene phytosterols refer to phytosterols that do not have a polyoxyethylene group. From the viewpoint of the encapsulation rate of the water-soluble cosmetic ingredient, the specific liposome according to the present disclosure preferably contains at least cholesterol as the D component.

[0033] The content of the D component relative to the total mass of the specific liposome is preferably 0.05% by mass to 0.7% by mass, more preferably 0.1% by mass to 0.5% by mass, and more preferably 0.2% by mass to 0.5% by mass. It is more preferable that the content is 0.5% by mass to 0.5% by mass. By setting the total content of phytosterols other than cholesterol and polyoxyethylene phytosterol within the above numerical range, the proportion of liposomes having a single lamellar structure in the specific liposomes can be increased, and the encapsulation rate of the cosmetic ingredient contained in the liposomes can be further improved. In addition, in the present disclosure, "the total content of the D component relative to the total mass of the specific liposome" means the cholesterol content when the specific liposome contains only cholesterol as the D component, means the phytosterol content when the specific liposome contains only phytosterol as the D component, and means the total amount of these contents when the specific liposome contains cholesterol and phytosterol as the D component.

[0034] (Specific liposomes) -d / c- In general, liposomes containing ceramides tend to have an increased average particle size. In the specific liposome according to the present disclosure, when the content of component C is c and the total content of component D is d, the content ratio d / c of ​​the two is 1.4 to 2.5 by mass. By making d / c 1.4 to 2.5 by mass, it is possible to achieve a high encapsulation rate and a small particle size while containing component B. That is, in the specific liposome, the content ratio d / c of ​​the components C and D contained in the specific liposome is set to a range of 1.4 to 2.5 in terms of mass ratio. From the viewpoint of suppressing an increase in the particle size of the specific liposome and further improving the encapsulation rate of the cosmetic ingredient, d / c is from 1.4 to 2.5, preferably from 1.5 to 2.1, and more preferably from 1.7 to 2.0. In the present disclosure, the ratio (d / c) of the content (d) of component D to the content (c) of component C in the liposome-containing composition for cosmetics is calculated by rounding off to two decimal places.

[0035] (Oleic acid) The specific liposomes may contain oleic acid, which functions as a penetration enhancer for the aqueous active ingredient encapsulated in the specific liposomes. Oleic acid is preferably included in the oil phase of certain liposomes. When the specific liposome contains oleic acid, the content of oleic acid is preferably in the range of 0.01% by mass to 1.0% by mass, more preferably 0.05% by mass to 0.50% by mass, and even more preferably 0.025% by mass to 0.075% by mass, from the viewpoint of not impairing particle formation and achieving a better penetration-enhancing effect.

[0036] The average particle size of the specific liposomes contained in the composition of the present disclosure is preferably 150 nm or less. By making the average particle size of the specific liposomes 150 nm or less, the transparency of the liposome-containing cosmetic composition containing the specific liposomes is improved, resulting in a more excellent appearance. In addition, when the liposome-containing cosmetic composition is applied as a cosmetic, for example, the permeability into the skin can be improved. On the other hand, by maintaining the above content ratio, the composition of the present disclosure is prevented from increasing in particle size and the proportion of liposomes having a single lamellar structure is increased, thereby preventing a decrease in the encapsulation rate of the beauty ingredient, resulting in a liposome-containing composition for cosmetics in the form of fine particles with a good encapsulation rate. There is no particular lower limit to the average particle size of the specific liposomes, but from the viewpoint of temporal stability and suppression of aggregation and coalescence of the specific liposomes over time, it can be set to 50 nm or more. From the viewpoint of improving the encapsulation rate of the cosmetic ingredient without increasing the turbidity of the composition, the average particle size of the liposome is preferably 150 nm to 50 nm, more preferably 130 nm to 70 nm, and even more preferably 110 nm to 90 nm. stomach.

[0037] From the viewpoint of improving the encapsulation rate of the cosmetic ingredient, the specific liposome is preferably a liposome having a single lamellar structure (also called a unilamellar vesicle or unilamellar liposome). The ratio of the total number of liposomes having a single lamellar structure to the total number of specific liposomes contained in the composition of the present disclosure is preferably 50% or more, and more preferably 70% or more. The liposome-containing cosmetic composition may contain liposomes having a multilamellar structure (also called multilamellar vesicles or multilamellar liposomes).

[0038] (Domestic and foreign waters) The liposome-containing cosmetic composition of the present disclosure comprises an internal aqueous phase and an external aqueous phase. The internal aqueous phase contains at least water and a cosmetic ingredient, and is present in a state encapsulated in the lipid bilayer membrane of the specific liposome. The external aqueous phase contains at least water.

[0039] (water) Examples of the water contained in the internal aqueous phase and the external aqueous phase include ion-exchanged water, pure water, purified water, and tap water. Among these, purified water is preferred in terms of its applicability to cosmetics.

[0040] The water content relative to the total mass of the liposome-containing composition for cosmetics is not particularly limited, but is preferably 1 mass% to 99 mass%, more preferably 10 mass% to 95 mass%, and even more preferably 20 mass% to 90 mass%. In the present disclosure, the "content of water relative to the total mass of the liposome-containing composition for cosmetics" means the sum of the content of water contained in the internal aqueous phase and the content of water contained in the external aqueous phase relative to the total mass of the liposome-containing composition for cosmetics.

[0041] (ethanol) The internal aqueous phase and the external aqueous phase may contain ethanol as necessary. Ethanol has good interactivity with ceramides contained in the specific liposomes, and by containing ethanol, the formability of the specific liposomes and the solubility of lipids can be further improved. When the liposome-containing composition for cosmetics contains ethanol, the content of ethanol may be 0.2% by mass to 30% by mass with respect to the total mass of the liposome-containing composition for cosmetics from the viewpoint of particle formability. The content of ethanol relative to the total mass of the liposome-containing composition for cosmetics may be 3 mass % or more, 5 mass % or more, or 10 mass % or more. Furthermore, the content of ethanol relative to the total mass of the liposome-containing composition for cosmetics may be 25% by mass or less, 20% by mass or less, or 18% by mass or less. In the present disclosure, the "ethanol content relative to the total mass of the liposome-containing composition for cosmetics" means the sum of the ethanol content contained in the inner aqueous phase and the ethanol content contained in the outer aqueous phase relative to the total mass of the composition of the present disclosure. Incidentally, ethanol includes absolute ethanol.

[0042] (Beauty ingredients) The specific liposome may contain a cosmetic ingredient. Cosmetic ingredients include ascorbic acid, ascorbic acid stearate, sodium ascorbate, disodium ascorbate sulfate, aspartic acid, aspartame, acetylglucosamine, acetylglutamic acid, acetylcysteine, acetylpantothenyl ethyl ester, disodium adenosine triphosphate, and dibasic adenosine monophosphate. Sodium, ε-aminocaproic acid, γ-aminobutyric acid, allantoin, allantoin-β-glycyrrhetinic acid, albumin, inositol, erythritol, glucosamine hydrochloride, pyridoxine hydrochloride, oxyproline, orotic acid, hydrolyzed elastin, caffeine hydrate, sodium chondroitin sulfate, cyanocobalamin, water-soluble elastin, taurine, sodium palmitoyl methyl taurine, sodium myristoyl methyl taurine, thiamine hydrochloride, theanine, deoxyribonucleic acid, ascorbyl palmitate, pantothenyl alcohol, sodium pantothenate, calcium pantothenate, pyridoxine, phytic acid, placenta extract, flavin adenine dinucleotide disodium dihydrate, anhydrous caffeine, riboflavin, riboflavin butyrate, riboflavin phosphate, resorcin, levulinic acid, glycine, arginine, lysine solution, lauroyl Aspartic acid, palmitoyl aspartic acid, cysteine, methionine, glutamic acid, threonine, serine, tyrosine, histidine, proline, glutathione, salicylic acid, nicotinamide, retinol palmitate, alginic acid, gamma-undecalactone, perilla oil, estradiol, estrone, ergocalciferol, beta-carotene, glucosamine, cholecalciferol, tocopherol acetate, retinoic acid oleic acid, salicylic acid, shikonin, ascorbyl dipalmitate, pyridoxine dipalmitate, natural vitamin E, α-tocopherol, horse oil, γ-nonalactone, bisabolol, vitamin A oil, hinokitiol, fumaric acid, powdered vitamin A, tocopherol linoleate, δ-tocopherol, tocopherol linoleate, isoleucine, phenylalanine, valine, leucine, ascorbyl tetra-2-hexyldecanoate, etc. Caffeine includes anhydrous caffeine.

[0043] From the viewpoint of solubility, the cosmetic ingredient contained in the internal aqueous phase is preferably a water-soluble cosmetic ingredient, and among the above, ascorbic acid, ascorbic acid stearate, sodium ascorbate, disodium ascorbate sulfate, aspartic acid, aspartame, acetylglucosamine, acetylglutamic acid, acetylcysteine, acetylpantothenyl ethyl ester, disodium adenosine triphosphate, disodium adenosine monophosphate, ε-aminocaproic acid, γ-aminobutyric acid, allantoin, allantoin β-glycyrrhetinic acid, albumin, inositol, erythritol, glucosamine hydrochloride, pyridoxine hydrochloride, oxyproline, orotic acid, hydrolyzed elastin, caffeine hydrate, sodium chondroitin sulfate, cyanocobalamin, water, Soluble elastin, taurine, sodium palmitoyl methyl taurine, sodium myristoyl methyl taurine, thiamine hydrochloride, theanine, deoxyribonucleic acid, ascorbyl palmitate, pantothenyl alcohol, sodium pantothenate, calcium pantothenate, pyridoxine, phytic acid, placenta extract, flavin adenine dinucleotide disodium dihydrate, anhydrous caffeine, riboflavin, riboflavin butyrate, riboflavin phosphate, resorcinol, levulinic acid, glycine, arginine, lysine solution, lauroyl lysine, aspartic acid, palmitoyl aspartic acid, cysteine, methionine, glutamic acid, threonine, serine, tyrosine, histidine, proline, glutathione, salicylic acid, nicotinamide, and the like are preferred. In addition, as beauty ingredients, plant extracts as natural ingredients, for example, Akebia extract, Ascophyllum nodosum extract, Asparagus extract, Aloe vera extract, Achillea millefolium extract, Avocado extract, Amacha extract, Sargassum extract, Arnica extract, Arnica flower extract, Apricot extract, Prunus mume fruit extract, Almond extract, Ginkgo biloba extract, Indian quince extract, Japanese butterbur extract, Japanese butterbur fruit extract, Turmeric rhizome extract, Udo extract, Hosta ventricosa extract, Glehnia littoralis extract, Goldthread root extract, Cinnamon extract, Carrot extract, Wild mugwort extract, Wild mugwort flower extract, Licorice extract, Licorice root extract, Licorice leaf extract, Kiwi extract, Cucumber extract, Gardenia jasminoides extract, Sasa kurilensis extract, Clara extract, Walnut extract Kiss, guava extract, grey extract, grapefruit extract, alpinia oleracea extract, gentiana extract, rice extract, fermented rice bran extract, fermented rice extract, comfrey leaf extract, sanshou extract, zanthoxylum extract, shiitake mushroom extract, perilla extract, perilla leaf extract, linden extract, meadowsweet extract, ginger extract, calamus root extract, honeysuckle extract, honeysuckle leaf extract, horsetail extract, stevia extract, yarrow extract, peppermint extract, peppermint leaf extract, hypericum perforatum flower / leaf / stem extract, se Hawthorn extract, Elderberry extract, Horse chestnut seed extract, Sage extract, Sage leaf extract, Sophora gracilis extract, Chinese laurel extract, Thyme extract, Dandelion extract, Tea leaf extract, Clove extract, Ginseng extract, Citrus aurantium extract, Camellia seed extract, Centella asiatica leaf extract, Asclepias persica leaf extract, Calendula officinalis leaf extract, Calendula officinalis root extract, Togo extract, Corn germ extract, Passiflora arbutus leaf extract, Tounin extract, Tosisou extract, Toraja extract, Aconite root extract, Aconite extract, Tonokina extract, Extract, Aoi seed extract, Houttuynia cordata extract, Peony extract, Jujube fruit extract, Nabara seed extract, Nanbari grass extract, Elderberry seed extract, Carrot root extract, Garlic extract, Cat's foot root extract, Cat's foot leaf extract, Lily seed extract, Saw palmetto extract, Nori extract, Centella asiatica extract, Capsicum extract, Angelica acutiloba extract, Calendula officinalis extract, Calendula officinalis flower extract, Tomato extract, Wild rose extract, Herb stem extract, Herb leaf extract, Hibiscus flower extract, Coix seed extract, Hamamelis extract, Haline Kiss, porcupine fish fruit extract, hahoniko extract, bakamonshiden extract, bucking powder extract, verbena seed extract, powder drink, papaya extract, barnyard millet extract, cypress fungus extract, hippophae rhamnoides fruit extract, sunflower seed extract, hydrangea extract, bulbul flower bud extract, phyco extract, fukisig extract, fukujinsou extract, grape leaf extract, blue noir extract, hemerocallis flower extract, hemp leaf extract, henna extract, henden extract, tuna extract, burdock extract, lotus extract, bakumondou extract,Birch extract, Japanese laurel extract, Japanese laurel leaf / stem extract, Fucus extract, Japanese cypress extract, Loquat extract, Loquat leaf extract, Coltsfoot extract, Tilia cordata flower extract, Poria columbine extract, Grape extract, Grape seed extract, Loofah extract, Hops flower extract, Cordia linden extract, Maca extract, Makomodake extract, Madonna lily flower extract, Morus alba root bark extract, Pine extract, Origanum majorana leaf extract, Mamaki extract, Mishimayuzu extract, Asian skunk cabbage extract, Miyamakirishima extract, Miyubiyou extract, Myrobalan seed extract, Muibai extract, Purple bark extract, Meadowfoam extract, Agate extract, Melissa extract, Peach extract, Mozuku extract, moringa seed extract, mondouju extract, moss bean seed extract, cornflower extract, palm leaf / flesh extract, fatsia leaf extract, mistletoe extract, eucalyptus extract, eucalyptus leaf extract, yuzu extract, lily extract, European white birch bark extract, European white birch bark extract, coix seed extract, mugwort extract, mugwort leaf extract, lavender extract, lavender flower extract, laurel extract, lychee extract, lemon extract, forsythia extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract, wild thyme extract, sanguisorba officinalis extract, and geranium extract are also preferred. The cosmetic ingredient may also be contained in the external aqueous phase.

[0044] The content of the cosmetic ingredient relative to the total mass of the liposome-containing composition for cosmetics is preferably 0.3% by mass to 5% by mass, and more preferably 0.5% by mass to 3% by mass.

[0045] (Other Ingredients) The internal aqueous phase and the external aqueous phase may contain other components such as physiological saline, sugar, pH adjusters, preservatives, etc. Examples of sugars include glucose, fructose, lactose, sucrose, trehalose, lactulose, and maltitol. Examples of pH adjusters include sodium hydrogen phosphate. Examples of preservatives include methyl paraoxybenzoate, etc. Examples of preservatives include sodium dihydrogen phosphate, disodium hydrogen phosphate anhydrous, sodium hydroxide (caustic soda), citric acid, acetic acid, triethanolamine, etc.

[0046] The pH of the inner aqueous phase and the outer aqueous phase is not particularly limited, but is preferably 5 to 9, more preferably 7 to 8. The pH of the inner aqueous phase and the outer aqueous phase can be adjusted by using the above-mentioned pH adjuster.

[0047] A method for producing the liposome-containing cosmetic composition of the present disclosure will be described below, but is not limited thereto. The liposome-containing cosmetic composition can be produced by mixing an oil phase composition and an aqueous phase composition, stirring and emulsifying the mixture to form specific liposomes.

[0048] By mixing, stirring and emulsifying the oil phase composition and the aqueous phase composition, the oil phase composition and the aqueous phase composition are emulsified into an O / W type (oil-in-water type) emulsion, and a liposome-containing composition for cosmetics is produced, which contains specific liposomes, an inner aqueous phase and an outer aqueous phase.

[0049] Examples of the stirring method include a method using ultrasonic waves or mechanical shearing force. In order to make the particle size uniform, an extruder treatment or a microfluidizer treatment can be performed by passing the mixture through a filter with a certain pore size. By performing an extruder treatment or the like, a multilayer liposome containing a plurality of unilamellar liposomes can be separated into a plurality of unilamellar liposomes.

[0050] The liquid temperature of the mixture of the oil phase composition and the aqueous phase composition can be adjusted as appropriate, but is preferably set at a temperature higher than the phase transition temperature of lecithin, which is component (A) contained in the mixture, for example, at 35°C to 70°C.

[0051] In producing a liposome-containing cosmetic composition, a solvent such as water and water may be evaporated from an aqueous solution containing liposomes. The evaporation mentioned above includes both intentional evaporation of a part or all of the solvent, and natural evaporation of a part or all of the solvent during the stirring and emulsification process.

[0052] When the solvent is intentionally evaporated, the evaporation method is not particularly limited, but examples thereof include a method of heating the solvent, a method of leaving the liposome-containing composition for cosmetic use at rest, a method of stirring the liposome-containing composition for cosmetic use, and a method of vacuum degassing.

[0053] The method for encapsulating the cosmetic ingredient in the liposome is not particularly limited, and can be carried out by using an aqueous phase composition in which the cosmetic ingredient is dissolved during emulsification.

[0054] The obtained liposome-containing cosmetic composition may be subjected to dialysis, filtration, extrusion, or other methods, which can make the average particle size of the liposomes contained therein uniform. The extrusion method is a method in which the liposome-containing cosmetic composition is passed through a filter having fine holes to apply physical shearing force and atomize the composition. When the liposome-containing cosmetic composition is passed through the liposome-containing cosmetic composition, the liposome-containing cosmetic composition and the filter are kept at a temperature equal to or higher than the phase transition temperature of lecithin, whereby the composition can be rapidly atomized.

[0055] (Application) In one embodiment, the liposome-containing composition for cosmetics of the present disclosure may be a cosmetic. The form of the cosmetic is not particularly limited, and may be a liquid, a jelly, a gel, a cream, a solid such as a stick, or a semi-solid. The liposome-containing composition for cosmetics of the present disclosure is preferably a transparent cosmetic. Examples of cosmetics include, but are not limited to, skin care cosmetics (skin lotions, milky lotions, beauty essences, sunscreens, etc.), body cosmetics (body lotions, etc.), scalp cosmetics, and facial packs.

[0056] All publications, patent applications, and standards mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent application, and standard was specifically and individually indicated to be incorporated by reference. EXAMPLES

[0057] The above embodiment will be specifically described below with reference to examples, but the above embodiment is not limited to these examples. In the examples, "%" means "% by mass" unless otherwise specified.

[0058] <Example 1> (Preparation of Oil Phase Composition) Ceramides (ceramide EOP: component B), hydrogenated soybean phospholipid (hydrogenated lecithin, PC content 90% or more: component A), polyoxyethylene phytosterol (average number of moles of oxyethylene groups added: 20, manufactured by Nikko Chemicals Co., Ltd., NIKKOL (registered trademark) BPS-20: component C), cholesterol (Nippon Fine Chemicals Co., Ltd., cholesterol JSQI: component D), ascorbic acid derivative (ascorbyl tetrahexyldecanoate, manufactured by Nikko Chemicals Co., Ltd., NIKKOL (registered trademark) VC-IP), and ethanol were mixed and dissolved by heating and stirring in a 60°C water bath for 10 minutes, to obtain an oil phase composition.

[0059] (Preparation of Aqueous Phase Composition) Anhydrous disodium hydrogen phosphate, sodium dihydrogen phosphate, methyl paraoxybenzoate and purified water were mixed and dissolved by heating and stirring in a 60° C. water bath for 1 hour to obtain an aqueous phase composition.

[0060] (Production of liposome-containing cosmetic composition) The aqueous phase composition and oil phase composition obtained above were mixed and dispersed for 1 hour using a Robomix Homomixer (manufactured by Tajima Chemical Machinery Co., Ltd.), and then two passes were made at 100 MPa using a high-pressure emulsifier (Starburst Mini, manufactured by Sugino Machine Co., Ltd.) to obtain a liposome-containing composition for cosmetics. The content of each component in the total mass of the liposome-containing cosmetic composition was as shown in Table 1. In the liposome-containing composition for cosmetics obtained in Example 1, the content ratio (d / c) of component D to component C was 1.74 on a mass basis. In Table 1, the ratio of the content of D component to the content of C component is shown as "d / c."

[0061] <Examples 2 to 6 and Comparative Examples 1 to 6> A liposome-containing cosmetic composition was produced in the same manner as in Example 1, except that the components of the liposome-containing cosmetic composition were changed as shown in Table 1. For Examples 2 to 6 and Comparative Examples 1 to 6, d / c is also shown in Table 1.

[0062] (Evaluation of liposome-containing compositions for cosmetics) For the liposome-containing compositions for cosmetics of Examples 1 to 6 and Comparative Examples 1 to 6, the encapsulation rate and the average particle size of the liposomes contained in the compositions were evaluated by the following methods.

[0063] <<Encapsulation rate evaluation (proportion of liposomes with single lamellar structure)>> The liposomes contained in the liposome-containing cosmetic compositions of the Examples and Comparative Examples were embedded in ice and TEM images were obtained at a magnification of 50,000 times using a transmission electron microscope.

[0064] The total number of liposomes present within a circle of radius 2 μm and the total number of liposomes having a single lamellar structure were counted at three arbitrary points on the TEM image, and the average ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome-containing cosmetic composition was confirmed and evaluated based on the following evaluation criteria. The evaluation results are shown in Table 1. Liposomes having a single lamellar structure are evaluated to have a higher encapsulation rate than liposomes having a multilamellar structure, even if they have the same average particle size. In the evaluation results section of Table 1, the encapsulation rate is described as "encapsulation rate (proportion of single-layer liposome content)."

[0065] (Evaluation Criteria) A: The proportion of liposomes having a single lamellar structure was 70% or more. B: The proportion of liposomes having a single lamellar structure was 50% or more and less than 70%. C: The ratio of liposomes having a single lamellar structure was 30% or more and less than 50%. D: The ratio of liposomes having a single lamellar structure was less than 30%. In the above evaluation criteria, from the viewpoint of improving the encapsulation rate of the beauty ingredient, ranks A to B are practically preferable levels.

[0066] <<Average particle size of liposomes>> The liposome-containing compositions for cosmetics produced in the Examples and Comparative Examples were diluted 10 times by mass with pure water, and the volume average particle size of the contained liposomes was measured by a dynamic light scattering method using a dynamic light scattering particle size measuring instrument (Analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.)) and evaluated according to the following evaluation criteria. The volume average particle size was measured within 20 to 24 hours after the production of the liposome-containing composition for cosmetics. The temperature of the measurement environment was 25° C. The measurement results are shown in Table 1. (Evaluation Criteria) AA: The volume average particle size was 110 nm or less. A: The volume average particle size was greater than 110 nm and less than 130 nm. B: The volume average particle size was more than 130 nm and was 150 nm or less. C: The volume average particle size exceeded 150 nm. In the above evaluation criteria, from the viewpoint of improving the encapsulation rate of the beauty ingredient and the appearance of the composition, ranks AA to B are practically preferable levels.

[0067] In the composition column of Table 1 below, "-" means that the component is not included. In Table 1, d / c for comparative examples not using component C and / or component D is marked as "-". In Table 1, polyoxyethylene phytosterols are listed with their trade names (BPS-20), and ascorbic acid derivatives are listed with their trade names (VC-IP).

[0068] [Table 1]

[0069] The results in Table 1 show that the liposome-containing compositions of Examples 1 to 6 have a finer average particle size, a larger proportion of liposomes having a single lamellar structure, and an excellent encapsulation rate of the cosmetic ingredient, compared to Comparative Examples 1 to 6. In addition, from the results in Table 1, in Comparative Example 3, which does not contain ceramides (component B), d / c is Since the particle size is smaller than the range specified in the table, the proportion of liposomes having a single lamellar structure is high, but it can be seen that the particle size is larger than that of Example 1, for example. Furthermore, the liposome-containing compositions of Comparative Examples 1, 2, and 6, which do not contain component D in the oil phase, have smaller particle sizes but lower encapsulation rates of the cosmetic ingredient than the Examples. The liposome-containing composition of Comparative Example 4, in which the oil phase contained components A to D but the d / c ratio was greater than the range specified in the present disclosure, had an increased liposome particle size. The liposome-containing composition of Comparative Example 5, in which d / c was greater than the range specified in the present disclosure, had a small average liposome particle size but was inferior in the encapsulation rate of the cosmetic ingredient.

[0070] Hereinafter, formulation examples in which the liposome-containing composition for cosmetics of the present disclosure is used as a cosmetic will be disclosed. In the following prescription examples, BG is an abbreviation for butylene glycol, PEG is polyethylene glycol, and PCA-Na is sodium pyrrolidone carboxylate. The names of these cosmetic ingredients are listed in detail in the "List of Cosmetic Ingredient Names" created by the Japan Cosmetic Industry Association.

[0071] <Example 7-1: Cream> A cream having the following composition was prepared by heating the oil phase and the water phase to 70°C and stirring while emulsifying (total amount 100% by mass). [Composition] [Content (mass%)] Liposome-containing composition of Example 5 0.8 Liposome-containing composition of Example 6 0.2 <Oily phase components> Squalane 8.0 Hydrogenated coconut oil 6.0 Cetanol 4.0 Neopentyl glycol dicaprate 4.0 Stearic Acid 3.0 Tocopherol 1.0 Polyoxyethylene sorbitan monooleate (20E.O.) 2.3 Lipophilic glyceryl monostearate 1.7 Sodium methyl stearoyl taurate 1.0 Tocopherol 1.0 ≪Aqueous phase components≫ BG 7.0 Arbutin 2.0 Dipotassium glycyrrhizinate 1.0 Magnesium Ascorbyl Phosphate 0.1 Phenoxyethanol 0.3 fragrance trace amount water remainder

[0072] The cream obtained in Example 7-1 had good properties in terms of feel when used, fragrance, etc., and it was found that the liposome-containing composition for cosmetics of the present disclosure can be suitably used for preparing creams.

[0073] <Example 7-2: Lotion> A lotion having the following composition was prepared by a conventional method (total amount 100% by mass). [Composition] [Content (mass%)] Liposome-containing composition of Example 5 0.8 Liposome-containing composition of Example 6 0.2 Glycerin 5.0 BG 5.0 Ethanol 3.0 PEG-32 1.0 N-Acetyl-L-Hydroxyproline 0.1 Water-soluble collagen 0.1 Hydrolyzed Collagen 0.1 Astaxanthin-containing emulsion composition (krill extract) [Composition prepared by the method described below] 0.2 Lycopene-containing emulsion composition [composition prepared by the method described above] 0.2 Carbomer 0.1 Phenoxyethanol 0.3 K hydroxide appropriate amount fragrance trace amount Water remaining

[0074] <Example 7-3: Lotion> A lotion having the following composition was prepared by a conventional method (total amount 100% by mass). [Composition] [Content (mass%)] Liposome-containing composition of Example 5 0.8 Liposome-containing composition of Example 6 0.2 Glycerin 10.0 BG 5.0 PEG-32 2.0 Diglycerin 1.0 N-Acetyl-L-Hydroxyproline 0.1 Water-soluble collagen 0.1 Hydrolyzed Collagen 0.1 Astaxanthin-containing emulsion composition (krill extract) [Composition prepared by the method described below] 0.2 Lycopene-containing emulsified composition [composition prepared by the aforementioned method] 0.2 Sodium hyaluronate 0.05 PCA-Na 0.1 Phenoxyethanol 0.3 Potassium hydroxide appropriate amount Fragrance trace amount Water balance

[0075] The lotions obtained in Example 7-2 and Example 7-3 had good properties in terms of usability, fragrance, etc., and it was found that the liposome-containing composition for cosmetics of the present disclosure can be suitably used for the preparation of lotions.

[0076] The lycopene-containing emulsified composition used in Example 7-2 and Example 7-3 was prepared by the following method.

[0077] <Preparation of lycopene-containing emulsified composition> (Preparation of oil phase composition a) The components of the following composition were weighed into a container and heated and mixed for 5 minutes with stirring on a hot plate at 150°C to obtain oil phase composition a. (Composition) · Tomato oleoresin (lycopene content 6% by mass) 2.8 parts by mass (manufactured by Sambright Co., Ltd., Lyc-O-Mato 6%) · Diglyceryl monostearate 0.3 parts by mass (manufactured by Nikko Chemicals Co., Ltd., NIKKOL® DGMS, HLB: 5.0) · Mixture of glyceryl tricaprylate and glyceryl tricaprate 11.3 parts by mass (manufactured by Kao Corporation, Coconaard® MT, HLB: 1) · Mixed tocopherol 0.6 parts by mass (manufactured by Riken Vitamin Co., Ltd., Riken E Oil 800)

[0078] (Preparation of aqueous phase composition a) The components of the following composition were weighed into a container and heated and mixed with stirring in a thermostat at 70°C to obtain aqueous phase composition a. (Composition) Decaglyceryl oleate 10 parts by mass (Nikko Chemicals Co., Ltd., Decaglyn 1-O) Glycerin 45 parts by weight Purified water 30 parts by weight

[0079] The obtained aqueous phase composition a was added to the oil phase composition a, and the mixture was stirred and mixed, and dispersed for a predetermined time using an ultrasonic homogenizer to obtain a crude dispersion. Thereafter, the obtained crude dispersion was subjected to high-pressure emulsification at 200 MPa using an ultra-high-pressure emulsifier (Ultimaizer, manufactured by Sugino Machine Ltd.) to prepare a lycopene-containing emulsion composition (lycopene content: 0.17% by mass).

[0080] The obtained lycopene-containing emulsion composition was diluted to 1% by mass with Milli-Q water, and the particle size of the dispersed particles was measured using a particle size analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.) to find that it was 52 nm.

[0081] <Example 7-4: Jelly-like serum> A jelly-like beauty essence having the following composition was prepared by a conventional method (total amount 100% by mass). [Composition] [Content (mass%)] Liposome-containing composition of Example 5 0.8 Liposome-containing composition of Example 6 0.2 Locust bean gum 0.3 Xanthan gum 0.3 Glycerin 5.0 Hydrogenated lecithin 0.5 Squalane 3.0 Dipotassium glycyrrhizinate 0.1 Astaxanthin-containing emulsion composition (krill extract) [Composition prepared by the method described below] 0.2 Ceramide NP, Ceramide AP mixture 1.0 Hydrolyzed Collagen 0.1 Acetylhydroxyproline 0.1 N-Acetyl-L-Hydroxyproline 0.1 Methylparaben 0.12 fragrance trace amount Water remaining

[0082] The jelly-like beauty serum obtained in Example 7-4 had good properties in terms of feel when used, fragrance, etc., and it was found that the liposome-containing composition for cosmetics of the present disclosure can be suitably used for preparing a jelly-like beauty serum.

[0083] The astaxanthin-containing emulsion compositions used in Examples 7-2, 7-3, and 7-4 were prepared by the following method.

[0084] <Preparation of Astaxanthin-Containing Emulsion Composition> (Preparation of aqueous phase composition b) The following components were dissolved for 1 hour while heating at 70° C. to obtain an aqueous phase composition b. (composition) · Sucrose stearate (HLB:16) 33.0g · Decaglyceryl monooleate (HLB:12) 67.0g · Glycerin 450.0g · Pure water 300.0g

[0085] (Preparation of Oil Phase Composition b) The following components were dissolved for 1 hour while heating at 70° C. to obtain oil phase composition b. (composition) · Krill extract 15.0g · Mixed tocopherol 32.0g (Riken Vitamin Co., Ltd., Riken E Oil 800) Medium chain fatty acid glyceride 93.0g (Kao Corporation, Coconard (registered trademark) MT) Lecithin 10.0g (Riken Vitamin Co., Ltd., Resion (registered trademark) P, derived from soybeans)

[0086] The aqueous phase composition B obtained above was stirred (10,000 rpm) using a homogenizer (HP93, manufactured by SMT Co., Ltd.) while keeping the temperature at 70°C, and then the oil phase composition b was added to the aqueous phase composition b to obtain a preliminary emulsion. The obtained preliminary emulsion was cooled to about 40°C and subjected to high-pressure emulsification at a pressure of 200 MPa using an Ultimizer HJP-25005 (manufactured by Sugino Machine Co., Ltd.).Then, the emulsion was filtered through a microfilter with an average pore size of 1 μm to prepare an astaxanthin-containing emulsion composition (astaxanthin content: 0.3% by mass).

[0087] The obtained astaxanthin emulsion composition was diluted to 1% by mass with Milli-Q water, and the particle size of the dispersed particles was measured using a particle size analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.) to find that it was 58 nm.

[0088] <Example 7-5: Sunscreen> A sunscreen agent having the following composition was prepared by a conventional method (total amount 100% by mass). [Composition] [Content (mass%)] Liposome-containing composition of Example 5 0.8 Liposome-containing composition of Example 6 0.2 <Oily phase components> Cyclomethicone 20.0 Ethylhexyl methoxycinnamate 7.0 HXMT-100ZA (Teika Co., Ltd., surface-treated titanium oxide particles, average primary particle size 15 nm) 6.0 Zinc oxide treated with triethoxycaprylylsilane 5.0 Isopropyl Isostearate 4.0 Glyceryl Stearate (SE) 2.0 Polysorbate 65 2.0 PEG-40 hydrogenated castor oil 1.0 Diethylamino hydroxybenzoyl hexyl benzoate 1.0 Tocopherol Acetate 0.5 ≪Aqueous phase components≫ BG 5.0 Astaxanthin-containing emulsion composition (krill extract) [Composition prepared by the method described above] 0.2 Centella asiatica extract 0.1 Phenoxyethanol 0.3 fragrance trace amount Water remaining

[0089] <Example 7-6: Sunscreen> A sunscreen agent having the following composition was prepared by a conventional method (total amount 100% by mass). [Composition] [Content (mass%)] Liposome-containing composition of Example 5 0.8 Liposome-containing composition of Example 6 0.2 <Oily phase components> Caprylic / Capric Triglyceride 12.5 Ethylhexyl methoxycinnamate 7.5 Diethylamino hydroxybenzoyl hexyl benzoate 5.0 Octocrylene 3.0 Cyclopentasiloxane 1.5 PEG-40 hydrogenated castor oil 1.0 Acrylates Copolymer Sodium 1.0 Haematococcus algae extract 0.1 ≪Aqueous phase components≫ Ethanol 20.0 Propylene glycol 5.0 Ceteareth-25 0.5 Hydrolyzed Collagen 0.1 Acetylhydroxyproline 0.1 Water-soluble collagen 0.1 Phenoxyethanol 0.3 fragrance trace amount Water remaining

[0090] The sunscreens obtained in Examples 7-5 and 7-6 had good properties in terms of usability, fragrance, etc., and it was found that the liposome-containing composition for cosmetics of the present disclosure can be suitably used for preparing sunscreens.

[0091] <Example 8: Cosmetic liposomes containing γ-aminobutyric acid in the internal aqueous phase> A liposome-containing cosmetic composition having the following composition can be produced in the same manner as in Example 1. [Composition] [Content (mass%)] <Oily phase components> Hydrogenated soy phospholipids 0.5500 Polyoxyethylene Phytosterol 0.1150 Cholesterol 0.2000 Tetra-2-hexyldecanoate ascorbyl ester 0.0500 Ceramides (Ceramide EOP, Ceramide AP, Ceramide NP) 0.0642 ≪Aqueous phase components≫ Absolute ethanol 10.0500 Sodium phosphate monobasic anhydrous 0.0650 Sodium dihydrogen phosphate 0.0030 Gamma-aminobutyric acid 8.0000 Methyl parahydroxybenzoate 0.2000 Water remaining

[0092] The liposome-containing composition for cosmetics obtained in Example 8 is a liposome-containing composition for cosmetics that contains a high encapsulation rate of γ-aminobutyric acid encapsulated in liposomes and also contains fine liposomes.

Claims

1. A liposome comprising a phospholipid, a ceramide, a polyoxyethylene phytosterol, and at least one of cholesterol and a phytosterol other than the polyoxyethylene phytosterol, The content of the polyoxyethylene phytosterol is c, When the total content of at least one of cholesterol and phytosterol other than polyoxyethylene phytosterol is d, A liposome-containing composition for cosmetics, comprising liposomes having a mass ratio d / c of ​​1.4 to 2.

5.

2. The liposome-containing cosmetic composition according to claim 1 , wherein the liposomes have an average particle size of 150 nm or less.

3. 3. The liposome-containing composition for cosmetics according to claim 1, wherein the ceramide is at least one selected from the group consisting of ceramide NDS, ceramide ADS, ceramide EODS, ceramide NS, ceramide AS, ceramide EOS, ceramide NP, ceramide AP, ceramide EOP, ceramide NH, ceramide AH, and ceramide EOH.

4. 3. The liposome-containing cosmetic composition according to claim 1, wherein the polyoxyethylene phytosterol has an average added mole number of oxyethylene groups of 10 or more.

5. 3. The liposome-containing composition for cosmetics according to claim 1, wherein the content of the polyoxyethylene phytosterol is 0.1% by mass or more based on the total mass of the liposome-containing composition for cosmetics.

6. 3. The liposome-containing composition for cosmetics according to claim 1, wherein a total content of the cholesterol and the phytosterols other than the polyoxyethylene phytosterol is 0.2% by mass to 0.5% by mass based on the total mass of the liposome-containing composition for cosmetics.

7. The liposome-containing cosmetic composition according to claim 1 , wherein the liposome comprises a liposome having a single lamellar structure.

8. The liposome-containing composition for cosmetics according to claim 1 or 2, which is a cosmetic.

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