Analysis method and analysis program

Abstract

To provide an analysis method and an analysis program capable of lightening a user's burden and also improving the determination precision of an analysis result.SOLUTION: An analysis method includes: dripping liquid to be analyzed on a micro fluid device which comprises an introduction part 3 for dripping the liquid and a plurality of flow passages extending from the introduction part, the flow passage including a region for analysis showing a coloring reaction corresponding to a component of the liquid and a region 4 for time measurement changing in color with the liquid; and analyzing the liquid by photographing the micro fluid device on which the liquid is dripped, by a measurement terminal comprising a camera part and a control part. The analysis method comprises a color change determination process of determining whether the region for time measurement changes into a predetermined color from image data of a photographed moving image.SELECTED DRAWING: Figure 5

Description

Technical Field

[0001] The present invention relates to an analysis method and an analysis program.

Background Art

[0002] Conventionally, a paper substrate device including a paper substrate, a hydrophobic silane coupling agent having a specific structure coated on the paper substrate, and a hydrophilic silane coupling agent having a specific structure coated within a region coated with the hydrophobic silane coupling agent to form a desired-shaped flow path is known (for example, Patent Document 1).

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] The paper substrate device described in Patent Document 1 and the like are generally called microfluidic paper-based analytical devices (μPADs), which are devices for performing analysis, chemical reactions, etc. using flow paths on paper. For example, by attaching a reagent that shows a color reaction with the liquid to be analyzed in the flow path, the concentration of one or more components to be analyzed can be determined using the color reaction.

[0005] Since the paper-based device can form a plurality of flow paths in one device, for example, multi-item simultaneous analysis is possible. However, when performing multi-item simultaneous analysis, the time required for analysis may vary depending on the analysis target. Therefore, when the user makes a visual judgment, it is necessary to accurately measure the time from the point when the liquid to be analyzed is dropped and confirm the color development of each analysis item at a predetermined time. There is a problem that accurate analysis cannot be performed due to human errors such as time measurement errors, measurement errors for each user, and overlooking the confirmation at the predetermined time.

[0006] Furthermore, during the analysis, the user is restricted, which causes a problem of a heavy burden on the user. Also, regarding the determination of the analysis results, it is necessary to compare the color of the colored part of the paper-based device with the color sample of the color reaction for determination. Especially in the case of multi-item simultaneous analysis, since a plurality of items are measured simultaneously, it is complicated, and there is a problem of a heavy burden on the user even when recording the analysis results.

[0007] The present invention has been made in view of such problems, and an object thereof is to provide an analysis method and an analysis program capable of reducing the burden on the user and improving the determination accuracy of the analysis results.

Means for Solving the Problems

[0008] The analysis method according to the present invention includes an introduction part for dropping a liquid and a plurality of flow paths extending from the introduction part. The flow paths include an analysis region that exhibits a color reaction according to the components in the liquid and a time measurement region whose color changes with the liquid. The liquid to be analyzed is dropped onto a microfluidic device, and the microfluidic device onto which the liquid has been dropped is photographed with a measurement terminal including a camera unit and a control unit to analyze the liquid. The method includes a video shooting step in which the camera unit shoots a video of the microfluidic device, a discoloration determination step in which the control unit determines whether or not the time measurement region has changed to a predetermined color from the image data of the video, a measurement start step in which the control unit starts measuring time when it is determined that the time measurement region has changed to the predetermined color, an analysis image shooting step in which the camera unit shoots analysis image data after a lapse of a predetermined time from the measurement start step, and an analysis step in which the control unit calculates an analysis result of the analysis region from the analysis image data.

[0009] In the analysis method according to the present invention, the microfluidic device is disposed on a mount sheet including a reference color region having an analysis color sample corresponding to the color after the color reaction in the analysis region. In the analysis image shooting step, the camera unit shoots the reference color region together with the microfluidic device. The analysis step may include a reference color information acquisition step of acquiring color information of the analysis color sample, a color information acquisition step of acquiring color information of the analysis region, and an analysis result calculation step of calculating the analysis result by comparing the color information of the analysis color sample with the color information of the analysis region.

[0010] In the analysis method according to the present invention, in the analysis result calculation step, a calibration curve of the color information of the analysis color sample may be created, and the analysis result may be calculated based on the calibration curve.

[0011] In the analysis method according to the present invention, a plurality of the predetermined times are set, and the analysis image shooting step may be repeatedly executed for each set predetermined time.

[0012] In the analysis method according to the present invention, the microfluidic device is disposed on a mount including a reference color region having a color sample for time measurement corresponding to the predetermined color. In the video shooting step, the camera unit shoots the reference color region together with the microfluidic device. The discoloration determination step includes a reference color information acquisition step of acquiring color information of the color sample for time measurement, a discoloration color information acquisition step of acquiring color information of the region for time measurement, and a comparison step of comparing the color information of the color sample for time measurement with the color information of the region for time measurement. In the comparison step, when the color difference between the color information of the color sample for time measurement and the color information of the region for time measurement is within a predetermined range, it may be determined that the region for time measurement has changed to the predetermined color.

[0013] The analysis program according to the present invention includes an introduction unit for dropping a liquid and a plurality of flow paths extending from the introduction unit. The flow paths are for causing a measurement terminal including a camera unit and a control unit to execute analysis of the liquid to be analyzed dropped onto a microfluidic device including a region for time measurement and an analysis region that exhibit a color reaction according to components in the liquid. The analysis program causes the measurement terminal to execute a video shooting process for causing the camera unit to shoot a video of the microfluidic device, a discoloration determination process for determining whether or not the region for time measurement has changed to a predetermined color from the image data of the video, a measurement start process for starting time measurement when it is determined that the region for time measurement has changed to the predetermined color, an analysis image shooting process for causing the camera unit to shoot analysis image data after a lapse of a predetermined time from the measurement start process, and an analysis process for calculating an analysis result of the analysis region from the analysis image data.

Advantages of the Invention

[0014] According to the present invention, it is possible to provide an analysis method and an analysis program capable of reducing the burden on the user and improving the determination accuracy of the analysis result.

Brief Description of the Drawings

[0015]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

[0016] Hereinafter, the best mode for carrying out the present invention will be described with reference to the drawings. Note that the following embodiments do not limit the invention according to each claim, and not all combinations of features described in the embodiments are essential for the solution means of the invention. Also, the drawings are schematic diagrams that have been appropriately emphasized, omitted, or adjusted in ratio to show the present invention, and may differ from the actual shape, positional relationship, and ratio.

[0017] [Configuration of the Measurement Device According to this Embodiment] First, the measurement device 1 according to the embodiment of the present invention will be outlined. As shown in FIG. 1, the measurement device 1 according to this embodiment includes a microfluidic device 2. The microfluidic device 2 according to this embodiment is a measurement device that exhibits a color reaction according to the components in the liquid. For example, it is a microfluidic paper-based analytical device (μPADs) in which a flow path for liquid analysis is provided on a paper substrate, or a device in which a flow path for liquid analysis is provided on a substrate such as glass or silicon. Further, the measurement device 1 includes a mounting board 6 on which the microfluidic device 2 can be placed.

[0018] In this embodiment, "analysis" means quantitative analysis or semi-quantitative analysis such as the concentration of the component to be analyzed. Also, in this embodiment, "mounting board" means a sheet-like member. That is, the mounting board 6 is not limited to cardboard, and can be composed of various arbitrary materials such as thin paper, plastic plates, metal plates, plastic or metal sheets. Further, the mounting board 6 may be a seal with an adhesive back surface that can be attached to a desk or the like.

[0019] The microfluidic device 2 includes a substantially circular introduction part 3 for dropping liquid, and a plurality of flow paths extending radially from the outer edge of the introduction part 3. Each flow path includes either an analysis region 5 that exhibits a color reaction according to the components in the liquid or a time measurement region 4 whose color changes with the liquid. The time measurement region 4 and the analysis region 5 are provided at the middle part and the end part of the flow path, and are formed in a substantially circular shape having a diameter larger than the width of the flow path.

[0020] As a method for forming the flow path of the microfluidic device 2, in addition to the method of forming the flow path wall on the substrate with a wax printer, a method of cutting the substrate into the shape of the flow path may also be used.

[0021] In some regions of the time measurement region 4 and the analysis region 5, predetermined reagents are attached respectively. The attachment of the reagent can be performed by impregnating a solution in which the reagent is dissolved into a predetermined region and then drying it. The type of reagent to be attached to one region may be one type or a plurality of types. Also, by performing the operation of impregnating and drying the solution in which the reagent is dissolved a plurality of times, a plurality of types of reagents may be attached to one region.

[0022] As shown in FIG. 2, the time measurement region 4 has a first region 4a (the middle part of the flow path) to which a time measurement reagent is attached, and a second region 4b which is located at the downstream end of the first region 4a of the flow path and to which no reagent is attached. As shown in FIG. 5, the second region 4b of the time measurement region 4 is configured such that when a liquid is dropped and the liquid flows to the second region 4b, it develops a predetermined color. In the present embodiment, the time measurement reagent is Acid Red 27, but it is not limited thereto, and any reagent that shows color development in the second region 4b can be adopted. Also, the time measurement reagent may be a reagent that shows a color reaction such as cobalt chloride, for example, and the time measurement region 4 may be configured to show a color reaction.

[0023] As shown in FIG. 1, the microfluidic device 2 of the measurement device 1 according to the present embodiment has a plurality of analysis regions 5 and is configured to be able to analyze a plurality of components contained in a liquid. Specifically, as shown in FIG. 2, for example, the microfluidic device 2 has a pH analysis region 51 for analyzing pH, a NO2-N (nitrite nitrogen) analysis region 52 for analyzing NO2-N, a K analysis region 53 for analyzing K (potassium), an NH3-N (ammonia nitrogen) analysis region 54 for analyzing NH3-N, a NO3-N (nitrate nitrogen) analysis region 55 for analyzing NO3-N, and a PO4 3― -P (phosphorus) analysis region 56 for analyzing PO4 3― -P.

[0024] The pH analysis area 51 has a third area 51a located in the middle of the flow path and a fourth area 51b located at the downstream end of the flow path relative to the third area 51a and to which no reagent is attached. Bromothymol blue (BTB) is attached to the third area 51a.

[0025] The NO2-N analysis area 52 has a fifth area 52a located in the middle of the flow path and a sixth area 52b located at the downstream end of the flow path relative to the fifth area 52a and to which no reagent is attached. Citric acid monohydrate, trisodium citrate dihydrate, urea, and N-1-naphthylethylenediamine dihydrochloride are attached to the fifth area 52a, and then L(+)-tartaric acid and sulfanilamide are attached.

[0026] The K analysis area 53 has a seventh area 53a located in the middle of the flow path and an eighth area 53b located at the downstream end of the flow path relative to the seventh area 53a and to which no reagent is attached. Sodium dipicrylamine is attached to the seventh area 53a.

[0027] The NH3-N analysis area 54 has a ninth area 54a located in the upper-middle part of the flow path, a tenth area 54b located in the lower-middle part of the flow path relative to the ninth area 54a, and an eleventh area 54c located at the downstream end of the flow path relative to the tenth area 54b. Sodium dichloroisocyanurate is attached to the ninth area 54a. Also, sodium hydroxide and sodium salicylate are attached to the tenth area 54b and the eleventh area 54c. In this way, by separately attaching the same reagent to the tenth area 54b and the eleventh area 54c, there is an advantage that even if a reagent with a high concentration is attached, the flow of the liquid is not inhibited and the liquid reaches the end of the flow path.

[0028] The NO3-N analysis area 55 has a 12th area 55a located in the middle part on the upstream side of the flow path, a 13th area 55b located in the middle part on the downstream side of the 12th area 55a of the flow path, and a 14th area 55c located at the end on the downstream side of the 13th area 55b of the flow path. Urea, N-1-naphthylethylenediamine dihydrochloride, L(+)-tartaric acid, and sulfanilamide are attached to the 12th area 55a. Vanadium chloride is attached to the 13th area 55b. Further, citric acid monohydrate is attached to the 14th area 55c.

[0029] PO4 3― The PO4-P analysis area 56 has a 15th area 56a located in the middle part of the flow path and a 16th area 56b located at the end on the downstream side of the 15th area 56a of the flow path. Trichloroacetic acid, hexaammonium heptamolybdate tetrahydrate, and citric acid monohydrate are attached to the 15th area 56a. After hydrochloric acid and stannous chloride are attached to the 16th area 56b, tartrazine is attached.

[0030] The microfluidic device 2 having the above configuration is used for, for example, the analysis of soil extracts and environmental waters such as rivers, lakes, and seas, but is not limited thereto, and various liquids can be the objects of analysis.

[0031] Note that the microfluidic device 2 may have only one analysis area 5 without having a plurality of them. Also, each of the above-described analysis areas 5 and the attached reagents are merely examples and are not limited thereto.

[0032] As shown in FIG. 1, the mount 6 includes a device placement area (not shown) for placing the microfluidic device 2, a reference color area 7 disposed around the device placement area, and a marker 8 disposed at the outer edge of the mount 6.

[0033] The microfluidic device 2 may simply be placed on the device placement area, may be detachably attached to the device placement area, or may be fixed to the device placement area.

[0034] The reference color area 7 is arranged so as to surround the microfluidic device 2 (device arrangement area), and has a time measurement color sample 7a corresponding to a predetermined color after color development in the time measurement area 4 of the microfluidic device 2, and an analysis color sample 7b corresponding to the color after the color reaction in the analysis area 5 of the microfluidic device 2.

[0035] The time measurement color sample 7a is arranged in the vicinity of the second area 4b of the time measurement area 4. Further, the analysis color sample 7b includes a color chart corresponding to the color reaction in each analysis area 5. Specifically, as shown in FIG. 3, the analysis color sample 7b includes a pH analysis color chart 71 corresponding to the color after the color reaction in the fourth area 51b of the pH analysis area 51, a NO2-N analysis color chart 72 corresponding to the color after the color reaction in the sixth area 52b of the NO2-N analysis area 52, a K analysis color chart 73 corresponding to the color after the color reaction in the eighth area 53b of the K analysis area 53, an NH3-N analysis color chart 74 corresponding to the color after the color reaction in the eleventh area 54c of the NH3-N analysis area 54, a NO3-N analysis color chart 75 corresponding to the color after the color reaction in the fourteenth area 55c of the NO3-N analysis area 55, and 3― a PO4 3― -P analysis color chart 76 corresponding to the color after the color reaction in the sixteenth area 56b of the -P analysis area 56.

[0036] The pH analysis color chart 71 has a first pH analysis color sample 71a, a second pH analysis color sample 71b, a third pH analysis color sample 71c, and a fourth pH analysis color sample 71d. The NO2-N analysis color chart 72 has a first NO2-N analysis color sample 72a, a second NO2-N analysis color sample 72b, a third NO2-N analysis color sample 72c, and a fourth NO2-N analysis color sample 72d. Further, the K analysis color chart 73 has a first K analysis color sample 73a, a second K analysis color sample 73b, a third K analysis color sample 73c, and a fourth K analysis color sample 73d.

[0037] Similarly, the NH3-N analysis color chart 74 has a first NH3-N analysis color sample 74a, a second NH3-N analysis color sample 74b, a third NH3-N analysis color sample 74c, and a fourth NH3-N analysis color sample 74d. Also, the NO3-N analysis color chart 75 has a first NO3-N analysis color sample 75a, a second NO3-N analysis color sample 75b, a third NO3-N analysis color sample 75c, and a fourth NO3-N analysis color sample 75d. Furthermore, the PO4 3― -P analysis color chart 76 has a first PO4 3― -P analysis color sample 76a, a second PO4 3― -P analysis color sample 76b, a third PO4 3― -P analysis color sample 76c, and a fourth PO4 3― -P analysis color sample 76d.

[0038] In this embodiment, the analysis color samples 7b corresponding to the colors after the color reaction in each analysis region 5 are arranged clockwise, but are not limited thereto. The arrangement of the analysis color samples 7b can adopt various arbitrary arrangements. Also, in this embodiment, each color chart has four-color color samples, but is not limited thereto, and the number of color samples included in each color chart can adopt various arbitrary numbers.

[0039] In this embodiment, the markers 8 are provided one by one at the four corners of the measurement device 1, but are not limited thereto. The number and arrangement of the markers 8 can adopt various arbitrary configurations. Also, in this embodiment, the markers 8 are AR markers, and the measurement terminal 100 is configured to identify the region inside the four markers 8 as the marker inner region of the measurement device 1.

[0040] [Configuration of the Measurement Terminal According to this Embodiment] As shown in FIG. 4, the measurement terminal 100 according to this embodiment includes a camera unit 120 and a control unit 140. Further, the measurement terminal 100 includes a display unit 130 and a storage unit 150. The measurement terminal 100 may be, for example, a general-purpose terminal with a built-in camera such as a smartphone or a tablet terminal, or may be an electronic computer such as a notebook PC or a desktop PC with an external camera connected thereto, or may be a dedicated terminal for analyzing the measurement device 1.

[0041] The camera unit 120 includes at least one lens and one image sensor, and is configured to be able to photograph the measurement device 1. Further, the camera unit 120 has a video shooting function, and in the video shooting process described later, as shown in FIG. 5, it is configured to shoot a video of the microfluidic device 2. Furthermore, the camera unit 120 is configured to shoot the analysis image data 152 in the analysis image shooting process described later.

[0042] In this embodiment, the measurement terminal 100 deletes the video captured by the camera unit 120 without storing it in the storage unit 150, but is not limited thereto, and the video may be stored and saved in the storage unit 150. Also, in this embodiment, when the measurement terminal 100 repeatedly executes the analysis image shooting process, it is configured to overwrite and save at any time without storing all the analysis image data 152 in the storage unit 150, but is not limited thereto, and all the analysis image data 152 may be stored in the storage unit 150. Furthermore, the measurement terminal 100 may temporarily store the analysis image data 152 and delete it after calculating the analysis result 154 described later.

[0043] When photographing the measurement device 1, the user fixes the measurement terminal 100 (or the external camera if the camera unit 120 is an external camera) to a photographing jig such as a tripod so that the microfluidic device 2 and the mount 6 are within the angle of view of the camera unit 120, or holds the measurement terminal 100 (external camera) by hand and photographs the measurement device 1.

[0044] The display unit 130 has a display as a display device, and in addition to the screen display function normally required in the measurement terminal 100, for example, it displays the analysis result 154 and the like. Further, the display unit 130 may be configured by a touch panel. When the display unit 130 is configured by a touch panel, the user can, for example, perform an operation such as starting video shooting on the measurement terminal 100 by operating the display unit 130. Note that since the display unit 130 can adopt various known configurations, a detailed description thereof is omitted.

[0045] The control unit 140 is configured by an arithmetic processing unit and includes a device identification unit 142, a timing unit 144, and an analysis unit 146. The device identification unit 142 is configured to be able to execute a video shooting process for causing the camera unit 120 to shoot a video of the measurement device 1. Further, the device identification unit 142 is configured to be able to recognize the marker 8 of the mount 6 from the image data of the video.

[0046] Furthermore, the device identification unit 142 is configured to be able to determine whether or not the marker 8 of the mount 6 is recognized. When the device identification unit 142 does not recognize the markers 8 at the four corners of the measurement device 1, that is, when it is determined that the marker 8 does not appear within the angle of view of the video, the display unit 130 displays a message indicating that the measurement device 1 does not appear correctly within the angle of view of the video (indicating that the measurement device 1 cannot be photographed correctly).

[0047] By providing such a configuration, it is possible to prompt the user to adjust the shooting position and prevent the analysis result 154 from varying depending on the user who takes the shot.

[0048] The timing unit 144 is configured to be able to execute a discoloration determination process for determining whether or not the time measurement area 4 of the microfluidic device 2 has changed to a predetermined color from the image data of the video, and a measurement start process for starting time measurement when it is determined that the time measurement area 4 has changed to the predetermined color.

[0049] The color change determination process includes a reference color information acquisition process for acquiring the color information of the color sample 7a for time measurement in the reference color region 7 of the mount 6, a variable color information acquisition process for acquiring the color information of the time measurement region 4 of the microfluidic device 2, and a comparison process for comparing the color information of the color sample 7a for time measurement with the color information of the time measurement region 4. In the comparison process, the timer unit 144 is configured to determine that the time measurement region 4 has changed to a predetermined color when the color difference between the color information of the color sample 7a for time measurement and the color information of the time measurement region 4 is within a predetermined range.

[0050] Specifically, in the comparison process, the timer unit 144 is configured to acquire the color information of the color sample 7a for time measurement, calculate the color difference based on the color information, and determine whether the color difference is within a predetermined range. In the present embodiment, the color information includes RGB values, a red color difference (Pr value), and a blue color difference (Pb value). Further, in the present embodiment, the color information of the time measurement region 4 acquired by the timer unit 144 is the color information of the central portion of the second region 4b of the time measurement region 4.

[0051] As shown in FIG. 5, the analysis unit 146 is configured to be able to execute an analysis image capturing process for causing the camera unit 120 to capture analysis image data 152 after a lapse of a predetermined time from the measurement start process. The analysis image capturing process may capture a still image (analysis image data 152) separately from the moving image capturing process after a lapse of a predetermined time, or may acquire the image data at the time point when the predetermined time has elapsed as the analysis image data 152 from the moving image of the moving image capturing process.

[0052] Further, the analysis unit 146 is configured to be able to set a plurality of predetermined times. In the present embodiment, a plurality of predetermined times are set, and the analysis unit 146 is configured to repeatedly execute the analysis image capturing process for each set predetermined time. Specifically, the analysis unit 146 acquires the first analysis image data 152 after a lapse of the first predetermined time (for example, 15 seconds) from the measurement start process. Further, the analysis unit 146 acquires the second analysis image data 152 after a lapse of the second predetermined time (for example, 25 seconds) from the measurement start process.

[0053] Furthermore, the analysis unit 146 acquires the third analysis image data 152 after the elapse of a third predetermined time (for example, 35 seconds) from the measurement start process. Still further, the analysis unit 146 acquires the fourth analysis image data 152 after the elapse of a fourth predetermined time (for example, 45 seconds) from the measurement start process. Also, the analysis unit 146 acquires the fifth analysis image data 152 after the elapse of a fifth predetermined time (for example, 50 seconds) from the measurement start process.

[0054] Still further, the analysis unit 146 is configured to be able to execute an analysis process for calculating the analysis result 154 of the analysis area 5 of the microfluidic device 2 from the analysis image data 152. The analysis process includes a reference color information acquisition process for acquiring the color information of the color sample 7b for analysis of the reference color area 7 of the mount 6, a presentation color information acquisition process for acquiring the color information of the analysis area 5, and an analysis result calculation process for calculating the analysis result 154 by comparing the color information of the color sample 7b for analysis with the color information of the analysis area 5.

[0055] In the reference color information acquisition process, when calculating the analysis result 154 of the pH of the liquid, for example, the analysis unit 146 acquires the color information from the pH analysis color chart 71 (the first pH analysis color sample 71a, the second pH analysis color sample 71b, the third pH analysis color sample 71c, and the fourth pH analysis color sample 71d) of the color sample 7b for analysis of the reference color area 7. Also, when calculating the analysis result 154 of NO2-N contained in the liquid, the analysis unit 146 acquires the color information from the NO2-N analysis color chart 72 (the first NO2-N analysis color sample 72a, the second NO2-N analysis color sample 72b, the third NO2-N analysis color sample 72c, and the fourth NO2-N analysis color sample 72d), and when calculating the analysis result 154 of K contained in the liquid, the analysis unit 146 acquires the color information from the K analysis color chart 73 (the first K analysis color sample 73a, the second K analysis color sample 73b, the third K analysis color sample 73c, and the fourth K analysis color sample 73d).

[0056] Similarly, when calculating the analysis result 154 of NH3-N contained in the liquid, the analysis unit 146 obtains color information from the NH3-N analysis color chart 74 (the first NH3-N analysis color sample 74a, the second NH3-N analysis color sample 74b, the third NH3-N analysis color sample 74c, and the fourth NH3-N analysis color sample 74d). When calculating the analysis result 154 of NO3-N contained in the liquid, the analysis unit 146 obtains color information from the NO3-N analysis color chart 75 (the first NO3-N analysis color sample 75a, the second NO3-N analysis color sample 75b, the third NO3-N analysis color sample 75c, and the fourth NO3-N analysis color sample 75d). When calculating the analysis result 154 of PO4 3― -P in the liquid, the analysis unit 146 obtains color information from the PO4 3― -P analysis color chart 76 (the first PO4 3― -P analysis color sample 76a, the second PO4 3― -P analysis color sample 76b, the third PO4 3― -P analysis color sample 76c, and the fourth PO4 3― -P analysis color sample 76d).

[0057] Also, in the color information acquisition process, when calculating the analysis result 154 of the pH of the liquid, for example, the analysis unit 146 obtains the color information at the center of the fourth region 51b of the pH analysis region 51 in the analysis region 5. When calculating the analysis result 154 of NO2-N contained in the liquid, the analysis unit 146 obtains the color information at the center of the sixth region 52b of the NO2-N analysis region 52 in the analysis region 5. When calculating the analysis result 154 of K contained in the liquid, the analysis unit 146 obtains the color information at the center of the eighth region 53b of the K analysis region 53 in the analysis region 5.

[0058] Similarly, when calculating the analysis result 154 of NH3-N contained in the liquid, the analysis unit 146 obtains the color information at the center of the eleventh region 54c of the NH3-N analysis region 54 in the analysis region 5. When calculating the analysis result 154 of NO3-N contained in the liquid, the analysis unit 146 obtains the color information at the center of the fourteenth region 55c of the NO3-N analysis region 55 in the analysis region 5. When calculating the analysis result 154 of PO4 3― -P in the liquid, the analysis unit 146 obtains the color information from the PO4 3―Obtain the color information of the central part of the 16th area 56b of the -P analysis area 56.

[0059] Specifically, in the analysis result calculation process, the analysis unit 146 is configured to create a calibration curve CC of the color information of the analysis color sample 7b and calculate the analysis result 154 based on the calibration curve CC. For each component to be analyzed, the analysis unit 146 uses any one of the color information of the R value, G value, B value, Pr value, and Pb value as the vertical axis of the calibration curve CC. In this embodiment, in the pH analysis, the analysis unit 146 uses the Pb value as the vertical axis of the calibration curve CC, and for the analysis of NO2-N, NH3-N, and PO4 3― -P analysis, the Pr value is used as the vertical axis of the calibration curve CC, in the K analysis, the B value is used as the vertical axis of the calibration curve CC, and in the NO3-N analysis, the G value is used as the vertical axis of the calibration curve CC, but it is not limited thereto. Further, the analysis unit 146 may be configured to create a plurality of calibration curves CC with different color information as the vertical axis for the analysis of one component and calculate the analysis result 154 based on the calibration curve CC.

[0060] For example, in the case of pH analysis, the analysis unit 146 with such a configuration creates a calibration curve CC from the color information (for example, Pb value) obtained from four points (the first pH analysis color sample 71a, the second pH analysis color sample 71b, the third pH analysis color sample 71c, and the fourth pH analysis color sample 71d) of the pH analysis color chart 71 in the reference color information acquisition process as shown in FIG. 6, and inserts the color information of the central part of the 4th area 51b of the pH analysis area 51 of the analysis area 5 obtained in the presentation color information acquisition process onto the calibration curve CC, thereby calculating the pH analysis result 154.

[0061] Also, for example, in the case of NO2-N analysis, the analysis unit 146 creates a calibration curve CC from the color information (e.g., Pr value) obtained from four points (the first NO2-N analysis color sample 72a, the second NO2-N analysis color sample 72b, the third NO2-N analysis color sample 72c, and the fourth NO2-N analysis color sample 72d) of the NO2-N analysis color chart 72 in the reference color information acquisition process, and interpolates the color information at the center of the sixth region 52b of the NO2-N analysis region 52 of the analysis region 5 of the analysis target region 5 obtained in the target color information acquisition process onto the calibration curve CC, thereby calculating the analysis result 154 of the concentration of NO2-N.

[0062] Furthermore, in the analysis process, the analysis unit 146 can not only calculate the analysis result 154 of one analysis target region 5 from one analysis image data 152, but also calculate the analysis results 154 of a plurality of analysis target regions 5 from one analysis image data 152.

[0063] The analysis unit 146 having such a configuration calculates, for example, the analysis result 154 of pH from the first analysis image data 152. Also, the analysis unit 146 calculates the analysis result 154 of NO2-N from the second analysis image data 152. Furthermore, the analysis unit 146 calculates the analysis result 154 of PO4 3― -P from the third analysis image data 152. Additionally, the analysis unit 146 calculates the analysis results 154 of NH3-N and NO3-N from the fourth analysis image data 152. Also, the analysis unit 146 calculates the analysis result 154 of K from the fifth analysis image data 152.

[0064] Note that in this embodiment, the analysis unit 146 calculates the analysis result 154 from the analysis image data 152 each time the analysis unit 146 acquires the analysis image data 152, but it is not limited to this. The analysis unit 146 may calculate the analysis results 154 from each analysis image data 152 collectively after the analysis unit 146 has acquired a plurality of analysis image data 152.

[0065] Further, as shown in FIG. 5, the analysis unit 146 is configured to be able to execute analysis result display processing for displaying the calculated analysis result 154 on the display unit 130. In the present embodiment, the analysis unit 146 is configured to be able to display the analysis results 154 of a plurality of analysis regions 5 in a list on the display unit 130. For example, the analysis unit 146 displays on the display unit 130 in a list the analysis result 154 of the pH of the measurement device 1, the analysis result 154 of NO2-N, the analysis result 154 of P, the analysis result 154 of NH3-N, the analysis result 154 of NO3-N, and the analysis result 154 of K. Further, the analysis unit 146 stores the calculated analysis result 154 in the storage unit 150.

[0066] Note that the analysis unit 146 may not be able to display the analysis results 154 of a plurality of analysis regions 5 in a list.

[0067] The storage unit 150 is configured by a storage device and stores various data in a readable and writable manner. As shown in FIG. 4, the storage unit 150 stores the analysis image data 152, the analysis result 154, and the analysis program 156.

[0068] The analysis program 156 is an analysis program that causes the measurement terminal 100 to analyze the liquid to be analyzed dropped on the microfluidic device 2, and includes a video shooting process for causing the camera unit 120 of the measurement terminal 100 to shoot a video of the microfluidic device 2, a discoloration determination process for determining whether or not the time measurement region 4 has changed to a predetermined color from the image data of the video, a measurement start process for starting time measurement when it is determined that the time measurement region 4 has changed to the predetermined color, an analysis image shooting process for causing the camera unit 120 to shoot the analysis image data 152 after a lapse of a predetermined time from the measurement start process, and an analysis process for calculating the analysis result 154 of the analysis region 5 from the analysis image data 152, and causes the measurement terminal 100 to execute these processes.

[0069] [Explanation of the analysis method according to the present embodiment] A method for analyzing the measurement device 1 using the measurement terminal 100 according to this embodiment will be described with reference to FIG. 7. FIG. 7 is a flowchart showing an example of an analysis procedure of the measurement device 1 using the measurement terminal 100 according to this embodiment. The analysis method according to this embodiment is generally an analysis method in which a liquid to be analyzed is dropped onto the microfluidic device 2, and the microfluidic device 2 onto which the liquid has been dropped is photographed with the measurement terminal 100 to analyze the liquid.

[0070] The analysis method according to this embodiment includes a video shooting step in which the camera unit 120 shoots a video of the microfluidic device 2, a discoloration determination step in which the control unit 140 determines whether or not the time measurement region 4 has changed to a predetermined color from the image data of the video, a measurement start step in which the control unit 140 starts measuring time when it is determined that the time measurement region 4 has changed to the predetermined color, an analysis image shooting step in which the camera unit 120 shoots analysis image data 152 after a lapse of a predetermined time from the measurement start step, and an analysis step in which the control unit 140 calculates an analysis result 154 of the analysis region 5 from the analysis image data 152.

[0071] This method will be described in detail below. In this embodiment, it is assumed that the microfluidic device 2 is placed in advance in the device placement area of the mount 6.

[0072] First, the user turns the camera unit 120 of the measurement terminal 100 toward the measurement device 1 and operates the measurement terminal 100 to select a start button for starting the analysis of the microfluidic device 2 (S1 in FIG. 7). The selection of the start button is, for example, a tap operation when the display unit 130 is configured by a touch panel.

[0073] After receiving the selection operation of the start button, the device identification unit 142 of the control unit 140 of the measurement terminal 100 causes the camera unit 120 of the measurement terminal 100 to start shooting (S10 in FIG. 7). The camera unit 120 shoots a video of the measurement device 1 (S11 in FIG. 7: video shooting step). Further, the device identification unit 142 recognizes the marker 8 of the mount 6 of the measurement device 1 from the image data of the video.

[0074] When the device identification unit 142 of the control unit 140 of the measurement terminal 100 does not recognize the four markers 8 on the mount 6 of the measurement device 1 (NO in S12 of FIG. 7), the display unit 130 of the measurement terminal 100 displays that the measurement device 1 is not correctly shown within the angle of view of the video, prompting the user to adjust the shooting position.

[0075] When the device identification unit 142 of the control unit 140 of the measurement terminal 100 recognizes the four markers 8 of the measurement device 1 (YES in S12 of FIG. 7), the video shooting process is continued as it is. In the video shooting process, when the device identification unit 142 correctly recognizes the marker 8, since the marker inner region of the measurement device 1 is shown in the angle of view of the camera unit 120 of the measurement terminal 100, the camera unit 120 shoots the reference color region 7 of the mount 6 together with the microfluidic device 2.

[0076] After the start of the video shooting process, the user drops a liquid (test liquid) for analysis onto the introduction part 3 of the microfluidic device 2 of the measurement device 1 (S2 in FIG. 7). Then, the timing unit 144 of the control unit 140 of the measurement terminal 100 determines whether the time measurement region 4 has changed to a predetermined color from the image data of the video (S13 in FIG. 7: discoloration determination process). Specifically, the timing unit 144 acquires the color information of the time measurement color sample 7a of the reference color region 7 of the mount 6 from the image data (S100 in FIG. 7: reference color information acquisition process). Also, the timing unit 144 acquires the color information of the second region 4b of the time measurement region 4 of the microfluidic device 2 (S101 in FIG. 7: discoloration information acquisition process).

[0077] Then, the timing unit 144 of the control unit 140 of the measurement terminal 100 compares the acquired color information of the time measurement color sample 7a of the reference color region 7 of the mount 6 with the color information of the time measurement region 4 of the microfluidic device 2 (S102 in FIG. 7: comparison process). When the color difference between the color information of the time measurement color sample 7a and the color information of the time measurement region 4 is within a predetermined range (YES in S102 of FIG. 7), the timing unit 144 determines that the time measurement region 4 has changed to a predetermined color and starts measuring time (S14 in FIG. 7: measurement start process).

[0078] On the other hand, when the color information of the time measurement color sample 7a and the color information of the time measurement area 4 of the control unit 140 of the measurement terminal 100 are not within a predetermined range (NO in S102 of FIG. 7), the color information of the time measurement color sample 7a and the color information of the time measurement area 4 are acquired again and compared.

[0079] Thereafter, the analysis unit 146 of the control unit 140 of the measurement terminal 100 waits until the analysis area 5 of the microfluidic device 2 of the measurement device 1 exhibits a color reaction (S15 in FIG. 7), and determines whether or not a predetermined time has elapsed (S16 in FIG. 7). When it is determined that the predetermined time has not elapsed (NO in S16 of FIG. 7), the analysis unit 146 waits again.

[0080] When it is determined that the predetermined time has elapsed (YES in S16 of FIG. 7), the analysis unit 146 of the control unit 140 of the measurement terminal 100 causes the camera unit 120 to capture the analysis image data 152. The camera unit 120 captures the analysis image data 152 in which the reference color area 7 is shown together with the microfluidic device 2 (S17 in FIG. 7: analysis image capturing step). Then, the analysis unit 146 calculates the analysis result 154 of the analysis area 5 from the analysis image data 152 (S18 in FIG. 7: analysis step).

[0081] Hereinafter, the analysis step according to the present embodiment will be described in detail with reference to FIG. 8. Specifically, the analysis unit 146 of the control unit 140 of the measurement terminal 100 acquires the color information of the analysis color sample 7b of the reference color area 7 of the mount 6 from the analysis image data 152 (S200 in FIG. 8: reference color information acquisition step). Further, the analysis unit 146 acquires the color information of the analysis area 5 of the microfluidic device 2 from the analysis image data 152 (S201 in FIG. 8: color development information acquisition step). For example, when the analysis unit 146 calculates the analysis result 154 of the pH of the liquid, the analysis unit 146 acquires the color information of the fourth area 51b of the pH analysis area 51 of the analysis area 5.

[0082] Then, the analysis unit 146 of the control unit 140 of the measurement terminal 100 calculates an analysis result 154 by comparing the color information of the color sample 7b for analysis of the reference color region 7 of the mount 6 that has been acquired with the color information of the analysis region 5 of the microfluidic device 2 (for example, the color information of the fourth region 51b of the pH analysis region 51) (S202 in FIG. 8: analysis result calculation step). In the analysis result calculation step, the analysis unit 146 creates a calibration curve CC of the color information of the color sample 7b for analysis, and calculates the analysis result 154 based on the calibration curve CC.

[0083] Thereafter, the analysis unit 146 of the control unit 140 of the measurement terminal 100 determines whether or not all the analyses of the liquid have been completed (S19 in FIG. 7). If it is determined that not all the analyses have been completed (NO in S19 in FIG. 7), the analysis unit 146 determines again whether or not a predetermined time (for example, a second predetermined time) has elapsed, and repeats the analysis image capturing step and the analysis step.

[0084] Also, when the analysis unit 146 of the control unit 140 of the measurement terminal 100 determines that all the analyses have been completed (YES in S19 in FIG. 7), it displays the calculated analysis result 154 on the display unit 130 of the measurement terminal 100 (S20 in FIG. 7: analysis result display step). Through the above steps, a series of analysis methods of the measurement device 1 by the measurement terminal 100 according to the present embodiment are executed.

[0085] [Advantages of the analysis method and analysis program according to the present embodiment] As described above, the analysis method according to the present embodiment includes an introduction unit 3 for dropping a liquid and a plurality of flow paths extending from the introduction unit 3. The flow paths include an analysis region 5 that exhibits a color reaction according to the components in the liquid and a time measurement region 4 whose color changes with the liquid. A liquid to be analyzed is dropped onto a microfluidic device 2, and the microfluidic device 2 onto which the liquid has been dropped is photographed with a measurement terminal 100 including a camera unit 120 and a control unit 140 to analyze the liquid. The method includes a moving image photographing step in which the camera unit 120 photographs a moving image of the microfluidic device 2, a discoloration determination step in which the control unit 140 determines whether or not the time measurement region 4 has changed to a predetermined color from the image data of the moving image, a measurement start step in which the control unit 140 starts measuring time when it is determined that the time measurement region 4 has changed to the predetermined color, an analysis image photographing step in which the camera unit 120 photographs analysis image data 152 after a lapse of a predetermined time from the measurement start step, and an analysis step in which the control unit 140 calculates an analysis result 154 of the analysis region 5 from the analysis image data 152.

[0086] And, by having such a configuration, the analysis method according to the present embodiment enables the measurement terminal 100 to automatically start measuring time based on the color reaction in the time measurement region 4, photograph the analysis image data 152 after a lapse of a predetermined time, and calculate the analysis result 154 from the analysis image data 152. Therefore, it is not necessary for the operator to measure a predetermined time with a clock or the like, and it is not necessary to visually determine the analysis result 154. As a result, there is no possibility of measurement errors in measuring a predetermined time or measurement errors between users, and the determination accuracy of the analysis result 154 can be improved. Further, since the analysis is automatically performed by the measurement terminal 100, the user can leave the site and perform another task by keeping the microfluidic device 2 onto which the liquid has been dropped in a state where it can be photographed by the camera unit 120, which has the advantage of reducing the burden on the user.

[0087] Also, in the analysis method according to the present embodiment, the microfluidic device 2 is disposed on a mount 6 including a reference color region 7 having an analysis color sample 7b corresponding to the color after the color reaction in the analysis region 5. In the analysis image capturing step, the camera unit 120 captures the reference color region 7 together with the microfluidic device 2. The analysis step includes a reference color information acquisition step of acquiring the color information of the analysis color sample 7b, a developed color information acquisition step of acquiring the color information of the analysis region 5, and an analysis result calculation step of calculating an analysis result 154 by comparing the color information of the analysis color sample 7b and the color information of the analysis region 5. By having such a configuration, the color information of the analysis color sample 7b and the color information of the analysis region 5 are acquired from the same analysis image data 152 and compared. Even if the shooting environment such as brightness and color temperature changes when shooting the analysis image data 152, the color information of the analysis color sample 7b and the color information of the analysis region 5 acquired in the same shooting environment are compared. As a result, the analysis result is not affected by the shooting environment, and thus has an advantage that the determination accuracy of the analysis result 154 can be improved.

[0088] Furthermore, in the analysis method according to the present embodiment, in the analysis result calculation step, a calibration curve CC of the color information of the analysis color sample 7b is created, and the analysis result 154 is calculated based on the calibration curve CC. By having such a configuration, the analysis result 154 can be calculated without being affected by the shooting environment, and thus has an advantage that the determination accuracy of the analysis result 154 can be improved.

[0089] Moreover, in the analysis method according to the present embodiment, a plurality of predetermined times are set, and the analysis image capturing step is repeatedly executed for each set predetermined time. By having such a configuration, even when analyzing the microfluidic device 2 having a plurality of analysis regions 5 with different predetermined times, the analysis image data 152 is automatically captured and the analysis result 154 is calculated for each predetermined time. Therefore, it has an advantage that the burden on the user can be reduced and the determination accuracy of the analysis result 154 can be improved.

[0090] Furthermore, in the analysis method according to the present embodiment, the microfluidic device 2 is disposed on a mount 6 including a reference color region 7 having a color sample 7a for time measurement corresponding to a predetermined color. In the video shooting step, the camera unit 120 shoots the reference color region 7 together with the microfluidic device 2. The discoloration determination step includes a reference color information acquisition step of acquiring color information of the color sample 7a for time measurement, a discoloration information acquisition step of acquiring color information of the region 4 for time measurement, and a comparison step of comparing the color information of the color sample 7a for time measurement with the color information of the region 4 for time measurement. In the comparison step, when the color difference between the color information of the color sample 7a for time measurement and the color information of the region 4 for time measurement is within a predetermined range, it is determined that the region 4 for time measurement has changed to a predetermined color. By providing such a configuration, the accuracy of determining whether the region 4 for time measurement has changed to a predetermined color is improved, and the measurement of time can be started at an accurate timing. Therefore, the measurement accuracy of a predetermined time is improved, and as a result, the determination accuracy of the analysis result 154 can be improved.

[0091] [Modification Example] As described above, the preferred embodiments of the present invention have been described. However, the technical scope of the present invention is not limited to the scope described in the above embodiments. Various changes or improvements can be made to the above embodiments.

[0092] For example, in the above-described embodiment, the microfluidic device 2 is disposed on a mount 6 including a reference color region 7 having an analysis color sample 7b corresponding to the color after the color reaction in the analysis region 5. In the analysis image capturing step, the camera unit 120 captures the reference color region 7 together with the microfluidic device 2. The analysis step has been described as including a reference color information acquisition step of acquiring the color information of the analysis color sample 7b, a developed color information acquisition step of acquiring the color information of the analysis region 5, and an analysis result calculation step of comparing the color information of the analysis color sample 7b with the color information of the analysis region 5 to calculate an analysis result 154, but is not limited thereto. For example, the microfluidic device 2 may not be disposed on the mount 6, and the camera unit 120 may capture only the microfluidic device 2, and the analysis result 154 may be calculated by comparing the color information of the analysis color sample data stored in the measurement terminal 100, the server, or the like with the color information of the analysis region 5.

[0093] In the analysis method according to the above-described embodiment, in the analysis result calculation step, a calibration curve CC of the color information of the analysis color sample 7b is created, and the analysis result 154 is calculated based on the calibration curve CC, but is not limited thereto. In the analysis result calculation step, the analysis method may not create a calibration curve CC of the color information of the analysis color sample 7b and calculate the analysis result 154 based on the calibration curve CC. For example, the information of the calibration curve CC stored in the measurement terminal 100, the server, or the like may be acquired, and the analysis result 154 may be calculated by comparing the acquired calibration curve CC with the color information of the analysis region 5.

[0094] In the above-described embodiment, a plurality of predetermined times are set, and the analysis image capturing step is repeatedly executed for each of the set predetermined times, but is not limited thereto. A plurality of predetermined times may not be set.

[0095] In the above-described embodiment, the microfluidic device 2 is disposed on a mount 6 including a reference color region 7 having a color sample 7a for time measurement corresponding to a predetermined color. In the video shooting step, the camera unit 120 shoots the reference color region 7 together with the microfluidic device 2. The discoloration determination step includes a reference color information acquisition step of acquiring the color information of the color sample 7a for time measurement, a discoloration information acquisition step of acquiring the color information of the region 4 for time measurement, and a comparison step of comparing the color information of the color sample 7a for time measurement with the color information of the region 4 for time measurement. In the comparison step, when the color difference between the color information of the color sample 7a for time measurement and the color information of the region 4 for time measurement is within a predetermined range, it has been described that it is determined that the region 4 for time measurement has changed to a predetermined color, but the present invention is not limited thereto. For example, the microfluidic device 2 is not disposed on the mount 6, the camera unit 120 shoots only the microfluidic device 2, and the color information of the color sample data for time measurement stored in the measurement terminal 100, the server, etc. is compared with the color information of the region 4 for time measurement. When the color difference between the color information of the color sample data for time measurement and the color information of the region 4 for time measurement is within a predetermined range, it may be determined that the region 4 for time measurement has changed to a predetermined color.

[0096] In the above-described embodiment, the measurement device 1 has been described as including a mount 6 on which the microfluidic device 2 can be placed, but the present invention is not limited thereto, and the measurement device 1 may not include the mount 6.

Explanation of reference numerals

[0097] 1 Measurement device 2 Microfluidic device 3 Introduction part 4 Region for time measurement 4a First region 4b Second region 5 Analysis region 51 Region for pH analysis 51a Third region 51b Fourth region 52 Region for NO2-N analysis 52a Fifth region 52b Sixth region 53 Region for K analysis 53a, 7th Region 53b, 8th Region 54, NH3-N Analysis Region 54a, 9th Region 54b, 10th Region 54c, 11th Region 55, NO3-N Analysis Region 55a, 12th Region 55b, 13th Region 55c, 14th Region 56, PO4 3― -P Analysis Region 56a, 15th Region 56b, 16th Region 6, Mounting Board 7, Reference Color Region 7a, Color Sample for Time Measurement 7b, Color Sample for Analysis 71, pH Analysis Color Chart 71a, 1st pH Analysis Color Sample 71b, 2nd pH Analysis Color Sample 71c, 3rd pH Analysis Color Sample 71d, 4th pH Analysis Color Sample 72, NO2-N Analysis Color Chart 72a, 1st NO2-N Analysis Color Sample 72b, 2nd NO2-N Analysis Color Sample 72c, 3rd NO2-N Analysis Color Sample 72d, 4th NO2-N Analysis Color Sample 73, K Analysis Color Chart 73a, 1st K Analysis Color Sample 73b, 2nd K Analysis Color Sample 73c, 3rd K Analysis Color Sample 73d, 4th K Analysis Color Sample 74, NH3-N Analysis Color Chart 74a, 1st NH3-N Analysis Color Sample 74b, 2nd NH3-N Analysis Color Sample 74c, 3rd NH3-N Analysis Color Sample 74d, 4th NH3-N Analysis Color Sample 75, NO3-N Analysis Color Chart 75a Color standard for the analysis of NO3-N, No. 1 75b Color standard for the analysis of NO3-N, No. 2 75c Color standard for the analysis of NO3-N, No. 3 75d Color standard for the analysis of NO3-N, No. 4 76 PO4 3― -P color chart for analysis 76a Color standard for the analysis of PO4-P, No. 1 3― -P color standard for analysis 76b Color standard for the analysis of PO4-P, No. 2 3― -P color standard for analysis 76c Color standard for the analysis of PO4-P, No. 3 3― -P color standard for analysis 76d Color standard for the analysis of PO4-P, No. 4 3― -P color standard for analysis 8 Marker 100 Measuring terminal 120 Camera unit 130 Display unit 140 Control unit 142 Device identification unit 144 Timing unit 146 Analysis unit 150 Memory unit 152 Image data for analysis 154 Analysis result 156 Analysis program CC Calibration curve

Claims

1. A microfluidic device comprising an introduction part for dropping a liquid and a plurality of flow paths extending from the introduction part, the flow paths including an analysis region showing a color reaction according to components in the liquid and a time measurement region whose color changes with the liquid, dropping the liquid to be analyzed onto the microfluidic device, and analyzing the liquid by photographing the microfluidic device onto which the liquid has been dropped with a measurement terminal having a camera part and a control part, the analysis method comprising: A video shooting step of shooting a video of the microfluidic device by the camera part; A discoloration determination step of determining by the control part whether or not the time measurement region has changed to a predetermined color from the image data of the video; When it is determined that the time measurement region has changed to the predetermined color, a measurement start step of starting time measurement by the control part; An analysis image shooting step of shooting analysis image data by the camera part after a lapse of a predetermined time from the measurement start step; An analysis step of calculating an analysis result of the analysis region from the analysis image data by the control part including The analysis method is characterized by the above.

2. The microfluidic device is disposed on a mount including a reference color region having an analysis color sample corresponding to the color after the color reaction in the analysis region, In the analysis image shooting step, the camera part shoots the reference color region together with the microfluidic device, The analysis step includes: A reference color information acquisition step of acquiring color information of the analysis color sample; A color development information acquisition step of acquiring color information of the analysis region; An analysis result calculation step of calculating the analysis result by comparing the color information of the analysis color sample with the color information of the analysis region including The analysis method according to claim 1, characterized by the above.

3. In the analysis result calculation step, a calibration curve of the color information of the color sample for analysis is created, and the analysis result is calculated based on the calibration curve. The analysis method according to claim 2, characterized in that.

4. A plurality of the predetermined times are set, and the analysis image capturing step is repeatedly executed for each set predetermined time. The analysis method according to any one of claims 1 to 3, characterized in that.

5. The microfluidic device is disposed on a mount sheet including a reference color region having a color sample for time measurement corresponding to the predetermined color. In the video capturing step, the camera unit captures the reference color region together with the microfluidic device. The discoloration determination step is as follows. A reference color information acquisition step of acquiring the color information of the color sample for time measurement. A discolored color information acquisition step of acquiring the color information of the time measurement region. And a comparison step of comparing the color information of the color sample for time measurement with the color information of the time measurement region. In the comparison step, when the color difference between the color information of the color sample for time measurement and the color information of the time measurement region is within a predetermined range, it is determined that the time measurement region has changed to the predetermined color. The analysis method according to any one of claims 1 to 3, characterized in that.

6. An analysis program for causing a measurement terminal including a camera unit and a control unit to analyze a liquid to be analyzed dropped onto a microfluidic device including an introduction unit for dropping the liquid and a plurality of flow paths extending from the introduction unit, the flow paths including a time measurement region and an analysis region that exhibit a color reaction according to the components in the liquid. The program includes: A video capturing process for causing the camera unit to capture a video of the microfluidic device. A discoloration determination process for determining whether or not the time measurement region has changed to a predetermined color from the image data of the video. When it is determined that the time measurement area has changed to the predetermined color, a measurement start process for starting time measurement, an analysis image capturing process for causing the camera unit to capture analysis image data after a lapse of a predetermined time from the measurement start process, an analysis process for calculating an analysis result of the analysis area from the analysis image data are executed on the measurement terminal An analysis program characterized by this.

Images