Oral composition

Abstract

To provide a barley young leaf-containing oral composition that has useful functions.SOLUTION: An oral composition contains barley young leaves, and at least two materials selected from protein, gymnema, acesulfame potassium and sucralose.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to an oral composition containing young barley leaves.

Background Art

[0002] In recent years, against the backdrop of the growing interest in health and the like, various health foods have been developed, and research has been conducted on various materials used in health foods.

[0003] For example, young barley leaves are generally used as a material for health foods and green juices, and have been widely studied for their functionality such as the effect of improving bowel movements and the action against hypercholesterolemia (Patent Documents 1 to 3).

[0004] Also, in recent years when there is a high preference for safety as well as a health orientation, products using safe materials such as young barley leaves that are consumed daily are preferred, and it is desired to make more use of the useful functions of such safe materials with food experience such as young barley leaves.

Prior Art Documents

Patent Documents

[0005]

Patent Document 1

Patent Document 2

Patent Document 3

Summary of the Invention

Problems to be Solved by the Invention

[0006] An object of the present invention is to provide an oral composition containing young barley leaves having useful functions.

Means for Solving the Problems

[0007] The inventors of the present invention have found that by using two specific other materials in young barley leaves, excellent myoblast proliferation effects and excellent α-glucosidase inhibitory effects can be obtained, and thus completed the present invention.

[0008] That is, the present invention is as follows. [1] An oral composition characterized by containing young barley leaves and at least two other materials selected from protein, Gymnema, acesulfame potassium, and sucralose. [2] The oral composition according to [1] above, which is characterized by being used for dieting.

Effects of the Invention

[0009] Since the oral composition of the present invention has excellent myoblast proliferation effects and / or excellent α-glucosidase inhibitory effects, it is effective for maintaining and enhancing muscle tissue, suppressing blood glucose level increase, and dieting.

Brief Description of the Drawings

[0010]

Figure 1

Figure 2

Modes for Carrying Out the Invention

[0011] The oral composition of the present invention is characterized by containing young barley leaves and at least two other materials selected from protein, Gymnema, acesulfame potassium, and sucralose.

[0012] The oral composition of the present invention has an excellent myoblast proliferation effect and can maintain and enhance muscle tissue. In addition, since muscle tissue is a tissue with active energy metabolism, maintaining and enhancing this muscle tissue can maintain and increase the basal metabolic rate, and thus obtain a diet effect by preventing and / or eliminating obesity.

[0013] Similarly, the oral composition of the present invention has an excellent α-glucosidase inhibitory effect and can delay the decomposition and absorption of sugar in vivo (intestinal tract), so it can suppress the rise in blood glucose level after meals. In addition, a diet effect can be obtained by delaying the decomposition and absorption of sugar in vivo (intestinal tract).

[0014] In addition, the oral composition of the present invention containing young barley leaves and at least two other raw materials has higher dispersibility than when using young barley leaves or when using young barley leaves and one other raw material. That is, the oral composition of the present invention is a powder that is difficult to agglomerate. Therefore, when dissolved in a liquid such as water or hot water and ingested, it is easily soluble and can be easily mixed. Furthermore, in the manufacturing process of the product, in the powder mixing process, adhesion between powders can be suppressed, formation of lumps can be suppressed, and uniform mixing becomes possible. That is, the oral composition with high dispersibility of the present invention can be prepared simply by mixing, and its preparation is also easy. For example, when mixing raw materials with strong cohesiveness and easy to form lumps, it is known that pretreatment such as crushing using a crusher is required for the raw materials to ensure their fluidity before charging, and after charging, it is necessary to supply air as quickly as possible so that they do not re-agglomerate and start flowing, but such complicated operations are not necessary. It is also known that in some cases, adhesiveness can be alleviated by pre-mixing a raw material with strong cohesiveness and easy to form lumps and an excipient, but such addition is not necessary either.

[0015] In addition, since the composition of the present invention has high dispersibility, even when mixing materials other than the components of the present invention to form the oral composition of the present invention, there is no need to perform complicated operations as described above, and it can be prepared simply by mixing.

[0016] [Barley young leaves] The barley (Hordeum vulgare L.) used as a raw material of the present invention is native to Central Asia and is an annual or biennial herb belonging to the Poaceae family. Depending on the ear shape, it is roughly classified into two-row barley, six-row barley, etc. The barley used in the present invention is not particularly limited as long as it is commonly available, and any barley such as two-row barley or six-row barley may be used. Also, any variety may be used. Barley young leaves may contain young leaves of barley and may also contain other parts such as stems.

[0017] Barley young leaves can be used as pulverized products, squeezed juices, extracts, etc. Examples of the pulverized products include dry powders, shredded products and their dried products (dried shredded products). The squeezed juice and extract may be in a liquid state, but can also be used as a paste or a dry powder (squeezed juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and as the solvent, for example, water (warm water, hot water), ethanol, or aqueous ethanol can be used. In the present invention, since the nutritional components contained in barley young leaves can be effectively utilized, it is preferably a dry powder obtained by drying and pulverizing barley young leaves. The dry powder preferably has 60% or more, more preferably 75% or more, and particularly preferably 90% or more of particles with a particle size of 75 μm or less.

[0018] [Other materials] (Protein) Protein is obtained by extracting and purifying proteins contained in animals and plants, and commercially available products can be used. In the present invention, a powdery form is preferred. Examples of animal-derived proteins include whey protein, casein protein, egg protein, beef protein, etc., and examples of plant-derived proteins include soy protein, pea protein, rice protein, etc. In the present invention, it is preferable to use plant-derived protein, and when combined with young barley leaves, it exhibits particularly excellent myoblast proliferation effect. Among them, it is particularly preferable to use soy-derived protein (soy protein). In addition, when combined with young barley leaves, soy protein exhibits particularly excellent dispersibility improvement effect. Therefore, soy protein preferably has a protein content of 50% or more, more preferably 70% or more, and particularly preferably 90% or more on a dry matter basis.

[0019] (Gymnema) Gymnema is a vine belonging to the Asclepiadaceae family native to India and Sri Lanka, and examples include Gymnema sylvestre. In the present invention, the preferred part to use is the leaf. Also, the stem may be included together with the leaf. Gymnema can be used as a pulverized product, juice, extract, etc. Examples of the pulverized product include dry powder, shredded pieces and their dried products (dried shredded pieces). The juice or extract may be in a liquid state, but can also be used as a paste or dry powder (juice powder, extract powder). The extract can be obtained by extracting with an appropriate solvent. Examples of the solvent include water (hot water), ethanol, and aqueous ethanol. In the present invention, the dry powder of Gymnema is preferred, and when combined with young barley leaves, it exhibits particularly excellent myoblast proliferation effect. Therefore, it is more preferable to use the dry powder of the hydrous alcohol extract of Gymnema leaves. In addition, the dry powder of Gymnema leaves exhibits particularly excellent dispersibility improvement effect when combined with young barley leaves. Therefore, the Gymnema acid content is preferably 5 to 80%, more preferably 10 to 60%, and particularly preferably 20 to 40%.

[0020] (Aceulfame Potassium) Aceulfame Potassium (Aceulfame K) is one of the artificial sweeteners and is a high-intensity sweetener with a sweetness intensity 200 times that of sucrose. Chemically, it is the potassium salt of 6-methyl-1,2,3-oxathiazin-4(3H)-one-2,2-dioxide. In the present invention, various forms such as liquid, powder, solid, granular, etc. can be used, and commercially available products can be used. In the present invention, the powdered form is preferred. When combined with young barley leaves, it exhibits an especially excellent effect of improving dispersibility. Therefore, it is preferable that 80% or more, more preferably 90% or more, and particularly preferably 99% or more of the particles have a particle size of 100 μm or less.

[0021] (Sucralose) Sucralose is a highly safe non-calorie high-intensity sweetener produced from sucrose and having a sweetness intensity about 600 times that of sucrose. In the present invention, various forms such as liquid, powder, solid, granular, etc. can be used, and commercially available products can be used. In the present invention, the powdered form is preferred. When combined with young barley leaves, it exhibits an especially excellent effect of improving dispersibility. Therefore, it is preferable that 80% or more, more preferably 90% or more, and particularly preferably 100% of the particles have a particle size of 20 mesh or less.

[0022] The oral composition of the present invention has, in addition to general foods, food additives, feeds, etc., an excellent effect of promoting the proliferation of myoblasts, maintaining and enhancing muscle tissue based on the α-glucosidase inhibitory effect, an effect of suppressing an increase in blood glucose level, and a diet (anti-obesity) effect. Therefore, for example, it can be used as so-called health foods such as functional foods whose efficacy has been approved by a prescribed agency such as foods for specified health use, foods with nutrient functions, and foods with functional claims, and pharmaceuticals (including quasi-drugs).

[0023] That is, the oral composition of the present invention can be used, for example, as an oral composition for dieting such as so-called health foods and pharmaceuticals. Such an oral composition for dieting contains young barley leaves and two other raw materials, and is not particularly limited as long as it can be distinguished from other products as a product in terms of being used for dieting. For example, any of the main body, packaging, instruction manual, and promotional materials of the product according to the present invention that displays the fact that it has a dieting function is included in the scope of the present invention. Note that the oral composition for dieting of the present invention is not limited to those in which the components of the present invention (young barley leaves and two other raw materials) are displayed as effective components for dieting on the packaging of the product or the like. For example, it may not specify the active ingredient, or it may display a specific raw material as the active ingredient.

[0024] Specifically, in so-called health foods, examples can include those displaying "For those concerned about weight", "Boost metabolism", "Help reduce body fat in those with a tendency to obesity", "Enhance the ability to metabolize fat", "Support the figure", etc.

[0025] Similarly, the oral composition of the present invention can be used as an oral composition for maintaining and enhancing muscle tissue and an oral composition for suppressing blood glucose level increase.

[0026] Examples of the form of the oral composition of the present invention include tablet form, capsule form, powder form, granule form, liquid form, granular form, rod form, plate form, block form, solid form, round form, paste form, cream form, caplet form, gel form, chewable form, stick form, etc. Among these, tablet form, capsule form, powder form, granule form, and liquid form are particularly preferred. Specifically, supplements, container-packed beverages filled in PET bottles, cans, bottles, etc., instant beverages (powdered beverages) to be dissolved in water (hot water), milk, fruit juice, green juice, etc. for drinking, and food additives can be exemplified. These are preferred in that they can be easily drunk during meals and can enhance palatability.

[0027] As for the contents of young barley leaves and two other materials (components of the present invention) in the oral composition of the present invention, they may be appropriately contained within the range where the effects are exhibited.

[0028] Generally, when the oral composition of the present invention is a supplement or pharmaceutical such as a tablet or a pill, it is preferable that the components of the present invention are contained in an amount of 0.01 to 100% by mass in terms of dry mass of the whole composition, more preferably 0.1 to 80% by mass, and even more preferably 1 to 60% by mass.

[0029] When the oral composition of the present invention is a container-packed beverage, it is preferable that the components of the present invention are contained in an amount of 0.001 to 30% by mass of the whole composition, more preferably 0.01 to 20% by mass, and even more preferably 0.1 to 15% by mass.

[0030] Also, when the oral composition of the present invention is an instant beverage such as green juice, it is preferable that the components of the present invention are contained in an amount of 0.01 to 100% by mass in terms of dry mass of the whole composition, more preferably 0.1 to 80% by mass, and even more preferably 1 to 60% by mass.

[0031] In order to more effectively exhibit the effects of the present invention, it is preferable that the components of the present invention are contained in an amount of 70% or more, more preferably 80% or more, even more preferably 90% or more, and particularly preferably 100% in terms of dry mass of the whole oral composition of the present invention.

[0032] Although there is no particular limitation on the intake amount of the oral composition of the present invention, from the viewpoint of more significantly exerting the effects of the present invention, it is preferably ingested so that the intake amount of young barley leaves in terms of dry mass is 0.01 g / day or more, more preferably 0.1 g / day or more, and even more preferably 1 g / day or more per adult per day. The upper limit is, for example, 30 g / day, preferably 20 g / day, and more preferably 10 g / day. The oral composition of the present invention can be contained in one container or divided into, for example, two to three containers as one-day intake so that the daily intake amount is the above-mentioned intake amount.

[0033] The blending mass ratio of young barley leaves and two other raw materials (total amount) is preferably 0.0001:1 to 10000:1, more preferably 0.001:1 to 1000:1, and even more preferably 0.01:1 to 100:1 in terms of dry mass conversion.

[0034] The oral composition of the present invention can be produced by a known method by adding other components other than the components of the present invention, if necessary. Examples of other components other than the components of the present invention include vitamins such as water-soluble vitamins (vitamin B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P), fat-soluble vitamins (vitamin A, D, E, K); minerals such as calcium, magnesium, phosphorus, iron; sulfur-containing compounds contained in taurine, garlic, etc.; flavonoids or flavonoid compounds such as hesperidin, quercetin; proteins such as collagen; peptides; amino acids; animal fats and oils; vegetable fats and oils; pulverized products or extracts of animals and plants, etc.

[0035] The oral composition of the present invention can be excellent as a composition for promoting myoblast proliferation, a composition for enhancing muscle strength, a composition for increasing muscle mass, a composition for activating energy metabolism, a composition for dieting, a composition for reducing adipose tissue, and a composition for reducing body fat by exhibiting the myoblast proliferation promoting effect shown in the examples described later. Further, by exhibiting the α-glucosidase inhibitory effect shown in the examples described later, it can be excellent as a composition for inhibiting α-glucosidase, a composition for suppressing blood glucose level elevation, and a composition for dieting. Furthermore, by exhibiting the effect of improving dispersibility shown in the examples described later, it can be a composition that is difficult to aggregate and has high dispersibility.

Examples

[0036] Hereinafter, the present invention will be described based on examples. [Example 1] (Raw materials) As the young barley leaves, a dried and powdered young barley leaf powder (90% or more with a particle size of 75 μm or less; manufactured by Toyo Shinyaku Co., Ltd.) was used. As the protein, a powdery isolated soy protein (protein content 90% or more on a dry matter basis; commercially available product) obtained by neutralizing curd obtained by acid precipitation of soy milk extracted from defatted soybeans, drying it, and passing it through a 20-mesh sieve was used. As Gymnema, Gymnema sylvestre powder (90% or more with a particle size of 40 mesh or less, 85% or more with a particle size of 80 mesh or less, Gymnemic acid content 25 - 30%; commercially available product) obtained by extracting the leaves of Gymnema sylvestre with hydrous ethanol, drying the extract, and powdering it was used. As acesulfame potassium, acesulfame potassium powder (particle size 100 μm; commercially available product) obtained by subjecting diketene, a substance derived from acetic acid, and sulfamic acid to a synthesis reaction, followed by a cyclization reaction with sulfuric acid and neutralization with potassium hydroxide, and then purification, concentration, and crystallization was used. As sucralose, sucralose powder (commercially available product) obtained by passing powdery sucralose through a 20-mesh sieve was used.

[0037] [Test Example 1] (Mouse myoblast proliferation test) In a 37°C, 5% CO2 incubator, using a 75 cm 2 flask, mouse myoblast cells (C2C12) were cultured with 10% FBS-DMEM. The cells suspended by trypsin treatment were transferred from the 75 cm 2 flask and seeded into each well of a collagen-coated 96-well plate at a cell density of 8000 cells per well.

[0038] After pre-incubation for 24 hours in a 37°C, 5% CO2 incubator, the test substance prepared with 10% FBS-DMEM was added to each well at 100 μL. Specifically, the preparation and addition of the test substance were performed as follows. First, the test substance was suspended in 10% FBS-DMEM to a concentration of 5 mg / ml and filtered through a 0.2 μm filter. Then, this suspension of the test substance at 5 mg / ml was further diluted with 10% FBS-DMEM and added to the wells to achieve the concentrations (μg / mL) shown in Table 1.

[0039]

Table 1

[0040] After culturing for 24 hours, the cells were washed twice with PBS and 100 μL of filter-sterilized MTT solution (MTT 500 μg / ml in DMEM) was added, followed by further culturing for 3 hours. The cells were washed twice with PBS and 100 μL of 0.04N HCl in isopropanol was added.

[0041] After confirming that the dye had eluted outside the cells, the absorbance (570 nm, 630 nm) was measured to determine the cell growth rate (% of control).

[0042] % of control = [SampleDate] / [ControlDate] × 100

[0043] Data = [(Sample570nm - Sample630nm) - (blank570nm - blank630nm)]

[0044] Sample570nm and blank570nm are the absorbance values at 570nm, and Sample630nm and blank630nm are the absorbance values at 630nm (turbidity).

[0045] The results are shown in Figure 1.

[0046] As shown in Figure 1, for the compositions of the present invention (Examples 1 to 4) in which two other materials were blended with young barley leaves, a remarkable improvement in cell proliferation rate was observed. In particular, when protein and sucralose were combined with young barley leaves (Example 3), and when gymnema and sucralose were combined with young barley leaves (Example 4), the increase was significant even compared to Examples 1 and 2. When using one kind of other material (Comparative Examples 6 to 9), no remarkable improvement in cell proliferation rate was observed compared to the comparative examples.

[0047] [Test Example 2] (α-Glucosidase Inhibition Test) Weighed each test substance into a 1.5 ml tube, added 0.1M PB to reach the predetermined final concentration shown in Table 2, and sonicated for 10 minutes. After centrifugation (10,000 rpm, room temperature, 5 min), the supernatant was collected as the sample solution.

[0048] [Table 2]

[0049] Added 80 μL / well of the sample solution (control is 0.1M PB) to a 96-well plate. Added 40 μL of the substrate solution and incubated at 37°C for 5 minutes.

[0050] Added 40 μL of the enzyme solution to the test wells, pre-incubated at 37°C for 15 minutes, and then added 160 μL of the reaction stop solution. For blank, after adding the reaction stop solution, 40 μL of the enzyme solution was added.

[0051] The absorbance at 440 nm was measured using a microplate reader. Using the measured absorbance, the inhibition rate was calculated using the following formula.

[0052] Inhibition rate (%) =(1 - [(Sample test - Sample blank ) / (Control test - Control blank )]) × 100

[0053] As the substrate solution, an aqueous solution of 0.02 M p-nitrophenyl α-D-glucopyranoside was used. As the enzyme solution, 0.02 U / ml α-glucosidase in 0.01 M PB (pH 7.0) containing 0.2% BSA was used. As the reaction stop solution, an aqueous solution of 0.2 M sodium carbonate was used.

[0054] The results are shown in Figure 2.

[0055] As shown in Figure 2, the composition of the present invention in which two other raw materials are blended with young barley leaves showed a synergistic improvement in the α-glucosidase inhibition rate.

[0056] [Test Example 3] (Dispersibility test) Each raw material was passed through a sieve (nominal size: 710). When combining two or more raw materials, the necessary amount for measuring the dispersibility was weighed according to the ratios in Tables 6 and 7, and mixed by hand in a plastic bag until uniform.

[0057] 10 g of the test substance was weighed, and the dispersibility was measured using the powder specific evaluation apparatus "Powder Tester PT-X" manufactured by Hosokawa Micron Corporation. The measurement of the dispersibility was performed according to the manual of the apparatus.

[0058] The dispersibility was measured by the following method. A sample of a specified weight (W) is dropped from a specified height onto a tray, and the weight (W1) of the sample on the tray is measured. Furthermore, the dispersibility is calculated by the following formula.

[0059] Dispersion degree(%) = (W - W1) / W × 100

[0060] The composition of each test substance and the results are shown in Tables 7 to 12.

[0061]

Table 3

[0062]

Table 4

[0063]

Table 5

[0064]

Table 6

[0065]

Table 7

[0066]

Table 8

[0067] As shown in Tables 3 to 8, the compositions of the present invention in which two other raw materials are blended with young barley leaves have higher dispersion degrees and improved dispersibility compared to young barley leaves alone.

[0068] [Formulation Example] A powdery edible composition (oral composition of the present invention) shown in Table 9 was prepared. When 3 g of the obtained composition of the present invention was mixed with 150 mL of water and drunk, the smell and taste of young barley leaves were improved, and a beverage with excellent overall palatability was obtained.

[0069]

Table 9

Industrial Applicability

[0070] Since the oral composition of the present invention can be used as so-called health foods and the like, it is industrially useful.

Claims

[Claim 1] An oral composition comprising barley grass, a protein, and a high-intensity sweetener.

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