Oral composition including glutathione

Abstract

To provide a pharmaceutical composition or a food composition which inhibits, improves, or prevents an increase in ALT due to alcohol ingestion, etc.SOLUTION: The inventors have found that ingestion of a combination of a polyphenol such as curcumin with glutathione results in an effect of lowering an ALT value.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a food composition and a pharmaceutical composition containing glutathione and polyphenols.

Background Art

[0002]

[0003] Glutathione is a tripeptide composed of cysteine, glutamic acid, and glycine, and is also called γ-L-glutamyl-L-cysteinyl-glycine. This glutathione is widely distributed in the living body, and the liver, spleen, adrenal glands, etc. contain particularly large amounts.

[0004] Glutathione is known to exhibit various functions in the living body. For example, antioxidant action by scavenging various reactive oxygen species (ROS), suppression of melanin production, whitening and beautifying effects by promoting collagen production, therapeutic effect on non-alcoholic fatty liver, detoxifying action on carbonyl compounds, etc.

[0005] Regarding the detoxifying action, the mechanism of the detoxifying action on carbonyl compounds has been elucidated. For example, carbonyl compounds such as methylglyoxal and glyoxal react non-enzymatically with the thiol group of glutathione and are metabolized by glyoxalase. NAD(P)H activates glutathione reductase and increases the glutathione concentration. It is known that a decrease in the concentration of redox coenzymes such as glutathione and NAD(P) results in a decrease in the detoxifying action on carbonyl compounds (Patent Document 1).

[0006] Since glutathione has various functions in this way, substances that promote glutathione production in the living body have been reported. For example, extracts of Portulaca oleracea (Patent Document 2), triterpenic acids or their derivatives (Patent Document 3), low molecular weight substances such as agar and agarose (Patent Document 4), etc. have been reported. In addition to promoting the production of glutathione, methods for enhancing the function of glutathione have also been proposed. For example, there are methods that utilize protein hydrolysates derived from rice bran or rice (Patent Document 5), methods that utilize yeast extracts containing glutathione (Patent Document 6), and the like. Cysteine is a type of amino acid and is not only used as a food additive, pharmaceutical, or cosmetic, but is also used as a synthetic precursor for coenzyme A, glutathione, and the like.

Prior Art Documents

Patent Documents

[0007]

Patent Document 1

Patent Document 2

Patent Document 3

Patent Document 4

Patent Document 5

Patent Document 6

Summary of the Invention

Problems to be Solved by the Invention

[0008] An object of the present invention is to provide a pharmaceutical composition or a food composition for suppressing, improving, or preventing an increase in ALT caused by alcohol intake or the like.

Means for Solving the Problems

[0009] The inventor of the present invention has found an effect of reducing the ALT value by ingesting polyphenols such as curcumin and glutathione in combination, and has completed the present invention. Specifically, (1) An oral composition containing polyphenols and glutathione as active ingredients (2) The composition of (1) for preventing disorders caused by alcohol intake (3) The composition of (1) for improving disorders caused by alcohol intake To provide.

Advantages of the Invention

[0010] According to the present invention, by taking polyphenols and glutathione in combination, the respective functionalities are improved, liver disorders caused by alcohol intake, lifestyle-related diseases, etc. are suppressed, and it can be used as a pharmaceutical composition, supplement, or food composition such as a functional food for suppressing, improving, or preventing an increase in ALT.

Brief Description of the Drawings

[0011]

Figure 1

Modes for Carrying Out the Invention

[0012] The pharmaceutical composition or food composition of the present invention is a composition containing polyphenols and glutathione. According to the present invention, by combining these, the functions can be exerted more effectively than each single product due to the synergistic effect.

[0013] Examples of the polyphenols of the present invention include plant extracts, plant pulverized products, etc. For example, flavonoids, chlorogenic acids, ellagic acids, lignans, curcuminoids, stilbenoids, coumarins, etc. are included. In particular, the polyphenols of the present invention are preferably extracts. In particular, organic solvent extracts are more preferable, and ethanol extracts are even more preferable. The polyphenol may be a mixture of two or more polyphenols.

[0014] As the polyphenols of the present invention, curcuminoids are particularly preferred, and as the curcuminoids, curcumin is suitable. There is no limitation on the available curcumin. In the present invention, natural products can be used. For example, curcumin may be extracted from natural plants containing curcumin. Also, those chemically synthesized by conventionally known methods or commercially available products may be used. As a method for extracting curcumin from natural plants, conventionally known methods can be used. For example, a method of extraction, concentration using ethanol or the like, and evaporation and removal of the solvent from the solvent fraction can be used.

[0015] The glutathione used in combination with the polyphenols in the present invention refers to reduced glutathione, oxidized glutathione, or a mixture thereof. Reduced glutathione represents a tripeptide having the structure of γ-L-Glu-L-Cys-Gly, and oxidized glutathione is a substance in which two molecules of reduced glutathione are bonded by an S-S bond. The form of glutathione may be anything as long as it contains glutathione as an active ingredient.

[0016] The glutathione of the present invention is not particularly limited as long as it is obtained by a method that can be used as a pharmaceutical or food. Usually, it is produced by extraction from microorganisms such as yeast, synthesis using enzymes, etc. Purified glutathione can also be used. Also, yeast or yeast extract containing glutathione may be used. Specifically, there is High Thione Extract YH-15 (manufactured by Kojin Life Science).

[0017] The method for producing the composition of the present invention can employ a general production method without particular limitation. A general production method used for producing it may be appropriately selected according to the form of the pharmaceutical composition or food composition. For example, if the form of the food composition is a powder, polyphenols, glutathione, and other components blended as necessary can be uniformly kneaded and produced. The blending amounts of the components other than glutathione and polyphenols may be determined as appropriate.

[0018] The intake amount of the present invention is not particularly limited, but is determined by the condition of the intake person, the selection of the composition to be administered, the age, weight, and response of the intake person, the condition of the intake person, etc. Generally, glutathione can be formulated so that it can be taken in an amount of 50 mg to 300 mg, more preferably 80 mg to 200 mg. For polyphenols, the recommended intake amount of the polyphenols used and the amount ingested in general foods may be appropriately adjusted. For example, since curcumin is recommended to be taken at 3 mg per 1 kg of body weight, it is formulated so that curcumin can be taken in an amount of about 50 mg to 200 mg, more preferably about 100 mg to 150 mg, per daily oral intake amount. The mixing ratio of glutathione and polyphenols can be adjusted in a timely manner so as to achieve the above intake amounts.

[0019] The intake method of the present invention is oral intake, but its form is not particularly limited, and it may be any oral preparation such as tablets, powders, granules, pills, suspensions, emulsions, infusions, decoctions, capsules, syrups, solutions, elixirs, extracts, tinctures, fluid extracts, etc.

[0020] The present invention can be ingested not only as a pharmaceutical, but also, for example, as a mixed food of glutathione-containing yeast extract and turmeric extract, and can also be ingested as a functional food, dietary supplement, supplement, etc.

[0021] In addition, for the present invention, compositions other than polyphenols and glutathione can be mixed with those generally added to pharmaceuticals and foods as long as the functions of the product of the present invention are not impaired. For example, dextrin, maltitol, sorbitol, starch, etc., which serve as excipients and diluents.

[0022] The composition of the present invention can be used as a composition for preventing or ameliorating disorders caused by alcohol consumption. After alcohol is consumed, it is decomposed into acetaldehyde in the body and further decomposed into acetic acid. In the composition of the present application, it is expected that the decomposition of acetaldehyde to acetic acid is promoted compared to when each is ingested alone. In the present invention, the retention of acetaldehyde in the body is meant as one of the disorders caused by alcohol consumption. Also, alcohol consumption is considered to be one of the causes of fatty liver, etc., but in the present invention, since the decrease in ALT value is promoted, it is considered to be effective in ameliorating fatty liver, etc. Therefore, the disorders caused by alcohol consumption in the present invention include a decrease in liver function.

Examples

[0023] The present invention will be specifically shown below, but the present invention is not limited thereto.

[0024] (Sample adjustment) The synergistic effect of glutathione (GSH: Mitsubishi Corporation Life Sciences) and curcumin (Curcumin: (FUJIFILM Wako Pure Chemical Corporation)) was confirmed. As shown in the graph of FIG. 1, curcumin was adjusted to samples at 10 μM, 25 μM, and 50 μM, glutathione was adjusted to 100 μg / ml, and the combined use of glutathione and curcumin was adjusted such that glutathione was 100 μg / ml and curcumin was 10 μM and 25 μM, respectively.

[0025] (ALT activity) 22×10 5 AML12 cells adjusted to a cell count of / ml were added to each well of a 24-well flat-bottom plate at 1 ml each and pre-cultured for 24 hours in an incubator at 37°C / 5% CO2. EtOH and a sample-containing medium were added, and further culture was performed. After 2 hours of culture, the ALT activity in the culture supernatant was measured.

[0026] The results are shown in FIG. 1. Compared with the control group without EtOH addition, an increase in ALT activity was observed in the EtOH-added group, and it was confirmed that the addition of GSH suppressed ALT activity. In addition, in the curcumin-added group, a concentration-dependent ALT activity inhibitory activity was observed, and furthermore, a higher ALT inhibitory activity was confirmed by simultaneously adding Curcumin and GSH compared to the single addition.

Claims

1. An oral composition containing polyphenols and glutathione as active ingredients

2. The composition according to Claim 1, which is for preventing disorders caused by alcohol intake

3. The composition according to Claim 1, which is for improving disorders caused by alcohol intake

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