Allergen-reducing composition for mites

Abstract

To provide an allergen-reducing composition for mites, exhibiting enhanced allergen reduction effect in mite applications.SOLUTION: An allergen-reducing composition for mites comprising dodecyl sulfate and tetradecyl sulfate, wherein each salt of the dodecyl sulfate and the tetradecyl sulfate is one or more selected from the group consisting of an alkali metal salt, an ammonium salt, and an ethanolamine salt, and wherein a weight-based blending ratio of the dodecyl sulfate to the tetradecyl sulfate is 90:10 to 50:50.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a composition for reducing allergens for mites.

Background Art

[0002] Allergic diseases such as bronchial asthma, atopic dermatitis, or allergic rhinitis, which have been increasing in recent years, are caused by various allergens present in the environment. Among them, the dead bodies and feces of mites are known as typical inhalant allergens. In particular, dust mites that inhabit fiber products such as carpets, bedding, curtains, sofas, etc. or tatami mats have become a major problem as a source of allergens.

[0003] As a composition for reducing allergens related to mites, a composition for reducing allergens containing dodecyl sulfate as an active ingredient has been developed (Patent Document 1).

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0005] Although dodecyl sulfate has an excellent effect of reducing allergens for mites, consumers have desired a more effective composition for reducing allergens for mites.

Means for Solving the Problems

[0006] As a result of intensive studies, the present inventor has found that by blending a specific amount of a specific dodecyl sulfate and tetradecyl sulfate, an allergen-reducing effect more excellent for mite applications can be obtained, leading to the present invention.

[0007] That is, the present invention is 〔1〕A composition for reducing allergens for mites, containing dodecyl sulfate and tetradecyl sulfate, wherein each salt of dodecyl sulfate and tetradecyl sulfate is one or more selected from the group consisting of alkali metal salts, ammonium salts, and ethanolamine salts, A composition for reducing allergens for mites, characterized in that the mixing ratio of dodecyl sulfate and tetradecyl sulfate is 90:10 to 50:50 by weight. 〔2〕The above composition for reducing allergens for mites, wherein the mixing ratio is 90:10 to 70:30 by weight. 〔3〕The above composition for reducing allergens, wherein each salt of dodecyl sulfate and tetradecyl sulfate is one or more selected from the group consisting of lithium salts, sodium salts, and potassium salts. 〔4〕A processing agent or treatment agent comprising the above composition for reducing allergens. To provide.

Effects of the Invention

[0008] According to the present invention, a composition for reducing allergens for mites having more excellent efficacy can be provided.

Modes for Carrying Out the Invention

[0009] The dodecyl sulfate used in the composition for reducing allergens for mites of the present invention is an alkali metal salt, ammonium salt, monoethanolamine salt, diethanolamine salt, or triethanolamine salt of dodecyl sulfate. Among these, preferably, it is a lithium salt, sodium salt, or potassium salt among the alkali metal salts of dodecyl sulfate, and more preferably, it is the sodium salt of dodecyl sulfate.

[0010] The tetradecyl sulfate used in the acarid allergen-reducing composition of the present invention is an alkali metal salt, ammonium salt, monoethanolamine salt, diethanolamine salt or triethanolamine salt of tetradecyl sulfate. Among these, preferably it is a lithium salt, sodium salt or potassium salt among the alkali metal salts of tetradecyl sulfate, and more preferably it is the sodium salt of tetradecyl sulfate.

[0011] The acarid allergen-reducing composition of the present invention is formulated such that the above-mentioned dodecyl sulfate and the above-mentioned tetradecyl sulfate are in a weight ratio of 90:10 to 50:50, preferably 90:10 to 70:30.

[0012] The acarid allergen-reducing composition of the present invention is usually used as a powder or a liquid preparation. Examples of the liquid preparation include an aqueous solution, an emulsion, a flowable agent, etc.

[0013] When making it into a liquid preparation, usually a solvent such as water or a polar solvent is used. Examples of the polar solvent include ethanol, propyl alcohol, isopropyl alcohol, butanol, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monopropyl ether, N-methylpyrrolidone, γ-butyrolactone, dimethyl sulfoxide, ethylene glycol, propylene glycol, butylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol with a molecular weight of 1000 or less, polypropylene glycol with a molecular weight of 1000 or less, etc. These polar solvents may be used alone or two or more of them may be used in combination.

[0014] Upon liquid formulation, one or more surfactants selected from nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants can be used. Among these, nonionic surfactants or anionic surfactants are preferred. Examples of nonionic surfactants include polyoxyethylene alkyl phenyl ether, polyoxyethylene styryl phenyl ether, polyoxyethylene alkyl ether, polyoxyethylene alkenyl ether, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, and the like. Examples of anionic surfactants include alkyl benzene sulfonate, polyoxyethylene alkyl phenyl ether sulfate, dialkyl sulfosuccinate, and the like. Examples of cationic surfactants include aliphatic amine salts and their quaternary ammonium salts, and the like. Examples of zwitterionic surfactants include betaine-type surfactants, aminocarboxylates, and the like.

[0015] The acarid allergen-reducing composition of the present invention may further contain other allergen-reducing agents, acarid control agents, antifungal agents, antibacterial agents, chelating agents, rust inhibitors, fragrances, antifoaming agents, antistatic agents, thickeners, softening agents, softeners, deodorants, disinfectants, adhesives, provided that the effects of the present invention are not impaired.

[0016] Examples of acarid control agents include pyrethroid compounds such as natural pyrethrin, phenothrin, permethrin, etc., organophosphorus compounds such as fenitrothion, malathion, fenthion, diazinon, etc., dicofol, chlorbenzilate, hexythiazox, tebufenpyrad, pyridaben, amidoflumet, benzyl alcohol, benzyl benzoate, phenyl salicylate, cinnamaldehyde, hyssop oil, carrot seed oil, and the like.

[0017] The allergen-reducing composition for mites of the present invention is, for example, kneaded with a fiber material or a non-woven fabric material and processed into fiber products such as carpets, bedding, curtains, sofas, etc., kneaded with tatami grass and processed into tatami mats, kneaded with a paper material and processed into paper products such as wallpaper and blinds, or kneaded with a resin component and processed into plastic products such as handrails and floor mats for use. Further, the allergen-reducing composition for mites of the present invention is blended into a spray agent, paint, coating agent, etc., and used after being treated by methods such as coating and spraying on fiber products, tatami mats, paper products, and plastic products.

[0018] The allergen-reducing composition for mites of the present invention is particularly effective against allergens derived from mites. In addition, it also has a certain effect on allergens derived from the hair and epithelium of pets such as dogs and cats, allergens derived from molds such as cockroaches and feathers, and allergens derived from pollen such as cedar, mugwort, giant hyssop, cypress, and plantain.

Examples

[0019] The present invention will be further described in detail by way of examples, but the present invention is not limited thereto.

[0020] According to the following table, various allergen-reducing compositions for mites were prepared. The unit is parts by weight.

[0021] 〔Table 1〕 TIFF2025110721000001.tif2993

[0022] The allergen-reducing composition was dissolved in a liquid containing ethanol and distilled water in a weight ratio of 1:1 so that the concentration became 10% by weight to prepare various liquid agents.

[0023] Regarding the above liquid agents, the mite allergen reduction rate was measured based on the following procedure. The results are shown in Table 2.

[0024] 〔Procedure for measuring the mite allergen reduction rate〕 1. The anti-Der f 2 monoclonal antibody 15E11 (manufactured by Fujifilm Wako Shibayagi Co., Ltd.) diluted to 2 μg / mL with PBS (manufactured by Fujifilm Wako Pure Chemical Corporation) was added to an F16 MAXISORP NUNC-IMMUNO MODULE plate (manufactured by NUNC) at 100 μL per well and left standing at 4°C for 3 days. 2. After standing, the liquid was discarded, and a blocking reagent {PBS (manufactured by Fujifilm Wako Pure Chemical Corporation) containing 1% by weight bovine serum albumin} was added to each well at 200 μL per well and left standing at 20 - 25°C for 60 minutes. 3. The liquid in the wells was discarded, and the plate was rinsed with PBS (manufactured by Fujifilm Wako Pure Chemical Corporation). 4. To 1 mL of an allergen solution containing the mite allergen Der f 2 {a solution prepared with PBS-T (manufactured by Fujifilm Wako Pure Chemical Corporation) to have a protein amount equivalent to 900 ng / mL of Der f 2}, 6.25 μL of the above-mentioned liquid agent was mixed and left standing at 20 - 25°C for 60 minutes. 5. The above-mentioned mixed solution was dropped at 100 μL per well and left standing at 20°C for 90 minutes. 6. The liquid in the wells was discarded, and the plate was rinsed with PBS (manufactured by Fujifilm Wako Pure Chemical Corporation). 7. The peroxidase-labeled anti-Der f 2 monoclonal antibody 13A4 (manufactured by Fujifilm Wako Shibayagi Co., Ltd.) was dissolved to 200 μg / mL with PBS-T (manufactured by Fujifilm Wako Pure Chemical Corporation) containing 1% by weight bovine serum albumin, and then diluted 2000-fold with PBS-T (manufactured by Fujifilm Wako Pure Chemical Corporation) containing 1% by weight bovine serum albumin. 8. The 2000-fold diluted solution was added to each well at 100 μL per well and left standing at 20°C for 90 minutes. 9. The liquid in the wells was discarded, and the plate was rinsed with PBS (manufactured by Fujifilm Wako Pure Chemical Corporation). 10. To 6.5 mL of 0.2 mol / L phosphate buffer (pH 6.2), add one tablet of ortho-phenylenediamine dihydrochloride (13 mg Tablet, manufactured by FUJIFILM Wako Pure Chemical Corporation) and 6.5 μL of 30% hydrogen peroxide solution, dissolve, and add 100 μL per well. Leave to stand at 37 °C for 10 minutes. 11. Add 50 μL per well of 2 mol / L sulfuric acid aqueous solution, and measure the absorbance (OD490 nm) using a microplate spectrophotometer (manufactured by Tecan Japan Co., Ltd.). Calculate the Der f 2-converted protein concentration from the absorbance, and calculate the mite allergen reduction rate according to formula (1).

[0025] Mite allergen reduction rate (%) = (900 - (Der f 2-converted protein amount)) / 900 × 100 (1)

[0026] Regarding the above liquid preparation, the Cryptomeria japonica pollen allergen reduction rate was measured based on the following procedure. The results are shown in Table 2.

[0027] 〔Procedure for measuring Cryptomeria japonica pollen allergen reduction rate〕 1. Add 100 μL per well of Cry j 1 monoclonal antibody 013 (manufactured by Biodynamics Laboratory Inc.) diluted to 2 μg / mL with PBS (manufactured by FUJIFILM Wako Pure Chemical Corporation) to an F16 MAXISORP NUNC-IMMUNO MODULE plate (manufactured by NUNC), and leave to stand at 4 °C for 1 day. 2. After standing, discard the liquid, add 200 μL per well of blocking reagent {PBS (manufactured by FUJIFILM Wako Pure Chemical Corporation) containing 1 wt% bovine serum albumin}, and leave to stand at 20 - 25 °C for 60 minutes. 3. Discard the liquid in the well, and rinse the plate with PBS (manufactured by FUJIFILM Wako Pure Chemical Corporation). 4. As the Cryptomeria japonica pollen allergen Cry j 1, 6.25 μL of the solution obtained by diluting each of the above-mentioned preparations 10-fold with ion-exchanged water with respect to 1 mL of the allergen solution {solution prepared with PBS-T (manufactured by FUJIFILM Wako Pure Chemical Corporation) so as to be 12.5 μg / mL} (hereinafter referred to as the standard Cryptomeria japonica pollen allergen solution) was mixed and allowed to stand at 20 to 25 °C for 60 minutes. 5. 100 μL of each of these mixed solutions was dropped per well and allowed to stand at 20 °C for 90 minutes. 6. The liquid in the well was discarded and the plate was rinsed with PBS (manufactured by FUJIFILM Wako Pure Chemical Corporation). 7. The peroxidase-labeled Cry j 1 monoclonal antibody 053 (manufactured by Biodynamics Laboratory Inc.) was dissolved in distilled water to 200 μg / mL, and the solution diluted 1200-fold with PBS-T (manufactured by FUJIFILM Wako Pure Chemical Corporation) containing 1 wt% bovine serum albumin was added 100 μL per well and allowed to stand at 20 °C for 90 minutes. 8. The liquid in the well was discarded and the plate was rinsed with PBS (manufactured by FUJIFILM Wako Pure Chemical Corporation). 9. 6.5 mL of 0.2 mol / L phosphate buffer (pH 6.2) to which ortho-phenylenediamine dihydrochloride (13 mg Tablet, manufactured by FUJIFILM Wako Pure Chemical Corporation) and 6.5 μL of 30% hydrogen peroxide solution were added was added 100 μL per well and allowed to stand at 37 °C for 10 minutes. 10. 50 μL of 2 mol / L sulfuric acid aqueous solution was added per well, and the absorbance (OD490 nm) was measured with a microplate spectrophotometer (manufactured by Tecan Japan Co., Ltd.). 11. The Cry j 1 concentration was determined from the absorbance, and the Cryptomeria japonica pollen allergen reduction rate was calculated by formula (2).

[0028] Cryptomeria japonica pollen allergen reduction rate (%) = (12.5 - (amount of Cry j 1)) / 12.5 × 100 (2)

[0029] [Table 2] TIFF2025110721000002.tif 2993 Reference Example

[0030] 〔Reference Example 1〕 Sodium dodecyl sulfate and sodium decyl sulfate were mixed so that the weight ratio was 70:30 to prepare an allergen-reducing composition.

[0031] 〔Reference Example 2〕 Sodium dodecyl sulfate and sodium hexadecyl sulfate were mixed so that the weight ratio was 70:30 to prepare an allergen-reducing composition.

[0032] Regarding the allergen-reducing compositions of Reference Example 1 and Reference Example 2, after preparing a solution by dissolving the allergen-reducing composition in a liquid containing ethanol and distilled water at a weight ratio of 1:1 so that the concentration of the allergen-reducing composition was 10% by weight, the mite allergen reduction rate was measured according to the above. The results are shown in Table 3.

[0033] TIFF2025110721000003.tif 1250

Claims

1. An acarid allergen-reducing composition containing dodecyl sulfate and tetradecyl sulfate, wherein each salt of dodecyl sulfate and tetradecyl sulfate is one or more selected from the group consisting of alkali metal salts, ammonium salts, and ethanolamine salts, and the mixing ratio of dodecyl sulfate and tetradecyl sulfate is 90:10 to 50:50 by weight, and the acarid allergen-reducing composition is characterized by this.

2. The acarid allergen-reducing composition according to Claim 1, wherein the mixing ratio is 90:10 to 70:30 by weight.

3. The allergen-reducing composition according to Claim 1 or 2, wherein each salt of dodecyl sulfate and tetradecyl sulfate is one or more selected from the group consisting of lithium salts, sodium salts, and potassium salts.

4. A processing agent or treatment agent comprising the allergen-reducing composition according to Claim 1 or 2.

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