Sanitary processing method

Abstract

To provide a sanitary processing method that can easily and reliably determine a point and a condition for sanitary processing, and can effectively execute sanitary processing, such as cleaning, disinfection, virus killing, and sterilization.SOLUTION: A sanitary processing method includes: detecting human saliva amylase in environment by immuno-chromatography; determining a point on which sanitary processing is performed and / or a condition for the sanitary processing on the basis of a result of the detection; and performing the sanitary processing on the basis of the determination.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a method for determining a sanitation treatment location, a method for determining sanitation treatment conditions, a method for determining a sanitation treatment method, a sanitation treatment method, a sanitation treatment kit, an estimation method, a method for displaying a contamination status at a candidate location for sanitation treatment, and a method for displaying a sanitation treatment location.

Background Art

[0002] The living environment surrounding us includes various facilities, installations, devices, etc., and various treatments are performed in consideration of their respective uses. For example, in commercial facilities, accommodation facilities, etc., treatments such as cleaning and sterilization are performed to maintain a hygienic state. At that time, it has been proposed to determine the degree of dirt, etc., and to determine treatment conditions, etc., according to the degree of dirt, etc.

[0003] Patent Document 1 discloses a cleaning method including a predetermined detection method for detecting a test substance such as a virus, and a cleaning step of removing the test substance from a target space according to the detection result of the detection method (Claim 19).

[0004] Patent Document 2 discloses a method for evaluating the hygienic state of a living environment, which includes a step of detecting the presence or absence of bacteria belonging to the genus Methylobacterium in the living environment.

[0005] Patent Document 3 discloses a method for determining the cleaning time of a toilet, and a method for determining the cleaning range of a toilet, which are composed of an installation step of arranging a specific toilet dirt detection sheet or a specific toilet dirt detection sheet laminate at a plurality of locations in a toilet area to be cleaned, a determination step of determining the degree of dirt at the plurality of locations from the discoloration state of the installed sheet, and a time determination step of determining the cleaning time of the toilet according to the determined degree of dirt.

Prior Art Documents

Patent Documents

[0006]

Patent Document 1

[0007] In an environment, when performing treatments such as cleaning, bleaching, deodorizing, virus killing, and sterilization, drugs corresponding to the treatment are often used. However, in various facilities, especially facilities such as commercial facilities, accommodation facilities, and manufacturing facilities, it is inefficient to apply drugs to all locations. That is, in such large-scale facilities, if the locations and times for performing treatments such as cleaning can be efficiently determined, labor and costs can be significantly reduced. In particular, since preventing infection by viruses and bacteria is important in facilities used by a large number of unspecified people, an effective method for reliably and efficiently performing the treatment is desired.

[0008] The present invention provides a method for determining a sanitation treatment, which can simply and reliably determine the locations and conditions where sanitation treatments such as cleaning, disinfection, virus killing, and sterilization can be effectively performed. [Means for Solving the Problems]

[0009] The present invention relates to a method for determining a location for a sanitation treatment, which detects amylase in an environment and determines the location for performing the sanitation treatment based on the detection result.

[0010] Further, the present invention relates to a method for determining a condition for a sanitation treatment, which detects amylase in an environment and determines the condition for the sanitation treatment based on the detection result.

[0011] Further, the present invention relates to a method for determining a sanitation treatment method, which detects amylase in an environment and determines the location for performing the sanitation treatment and the condition for the sanitation treatment based on the detection result.

[0012] The present invention also relates to a sanitation treatment method for determining at least one of a location for performing sanitation treatment and conditions for sanitation treatment by the determination method of the present invention, and performing sanitation treatment based on the determination.

[0013] The present invention also relates to a sanitation treatment kit including means for detecting amylase in the environment and a drug used for treating a sanitation treatment location determined based on a detection result of amylase obtained by the means.

[0014] The present invention also relates to an estimation method for detecting amylase in the environment and estimating a location where a harmful virus and / or harmful bacteria are likely to exist based on the detection result.

[0015] The present invention also relates to an estimation method for detecting amylase in the environment, estimating a location where a harmful virus and / or harmful bacteria are likely to exist based on the detection result, and estimating an infection route.

[0016] The present invention also relates to a method for displaying a contamination status at a candidate location for sanitation treatment, (a) A step of identifying one or more locations in the environment, the step including the following (i) to (iii) steps, (i) A step of generating position data capable of identifying the installation locations of physical objects to identify the one or more locations within a defined area, (ii) A step of recording the position data of the one or more locations in the environment, and (iii) A step of separately recording physical objects linked to the position data of the one or more locations in the environment by position and part, (b) A step of recording a detection result of amylase detected from one or more locations in the environment, (c) A step of recording the generated detection result separately by the position and part of the physical objects recorded in (iii) of (a), and (d) A step of displaying the recording in (c) together with the position data of the one or more locations in the environment, Relates to a method for displaying a contamination status at a candidate location for a sanitation treatment, comprising

[0017] Further, the present invention relates to a method for displaying a sanitation treatment location, which determines a sanitation treatment location based on the contamination status at a candidate location for a sanitation treatment displayed by the display method of the present invention and displays it as the sanitation treatment location.

Effect of the Invention

[0018] According to the present invention, there is provided a method for determining a sanitation treatment location, sanitation treatment conditions, or sanitation treatment method, which can easily and surely determine a location where sanitation treatments such as cleaning, disinfection, virus killing, and sterilization can be effectively carried out. Further, the present invention provides a method for easily and surely estimating the location and infection route of harmful viruses and / or harmful bacteria.

Brief Description of the Drawings

[0019]

Figure 1

Figure 2

Mode for Carrying Out the Invention

[0020] In this specification, "sanitation treatment" may be a treatment applied to the environment to maintain health. For example, it may mean removing "contaminants" from the "environment" and further from the "object", inactivating the "contaminants", or both. In this specification, "contaminant" may be a substance that causes a health disadvantage when present in an object. For example, it may include saliva, droplets from the mouth, etc. Droplets from the mouth may contain saliva and substances associated with saliva such as amylase. Further, the "substances associated with saliva" may include viruses and / or bacteria (sometimes simply referred to as "bacteria"), and further harmful viruses and / or harmful bacteria. As used herein, "contamination" may refer to a situation where, for example, in the "environment", and more specifically in the "object", contaminants such as saliva and substances associated with saliva such as amylase are present in an amount that is detrimental to health, as opposed to a situation where they are not present.

[0021] Amylase is an enzyme contained in human saliva. The present invention has found that the degree of detection of amylase adhering to the surface of the living environment correlates with the possibility of contamination by components containing amylase, and determines that a location with a high degree of amylase detection is a location with a high possibility of contamination, and determines that such a location should be a location for performing sanitation treatments such as cleaning, disinfection, virus killing, and sterilization, thereby finding that contamination by viruses and / or bacteria can be efficiently prevented and removed. Furthermore, the present invention has found that based on the degree of detection of amylase, the possibility of the presence of harmful viruses and / or harmful bacteria and the infection route can be accurately estimated. Determining the possibility of contamination based on the degree of detection of amylase, determining the location for performing sanitation treatments, and further estimating the possibility of the presence of harmful viruses and / or harmful bacteria and the infection route have not been conventionally performed.

[0022] In the present invention, amylase in the environment is detected. The environment may be a living environment, a natural environment, etc. The environment, especially the living environment, may include buildings and public transportation. Also, buildings and public transportation may each include the equipment associated therewith. The scale, use, etc. of the building are not limited.

[0023] Examples of the building include a building selected from a commercial facility, a lodging facility, an educational facility, a medical facility, a nursing facility, a business facility, and a food service facility, and these may be a complex facility. Examples of the commercial facility include a shopping center, a supermarket, a cinema complex, an amusement park, etc. Examples of the lodging facility include a hotel, a ryokan, a private lodging facility, etc. Examples of the educational facility include various schools, a cultural center, etc. Examples of the medical facility include a hospital. Examples of the nursing facility include a care house for the elderly, etc. Examples of the business facility include an office building, a factory, etc. Examples of the food service facility include a restaurant, a coffee shop, a fast food restaurant, etc.

[0024] Examples of the public transportation include a public transportation selected from a railway vehicle, a road vehicle, a ship, and an aircraft. Examples of the railway vehicle include a train, a diesel car, etc. Examples of the road vehicle include a bus, etc. Examples of the ship include a passenger ship, etc. Examples of the aircraft include a passenger aircraft, etc.

[0025] In the present invention, the environment includes an object for detecting amylase (hereinafter referred to as a detection object), and amylase can be detected on the surface of the detection object. The detection object may include, for example, a detection object selected from a building, building equipment, public transportation, and public transportation equipment. In the present invention, as the detection object, amylase can be detected on the surface of an article, for example, an article arranged indoors. The article may be a fixed article or a non-fixed article. The material of the article is not limited either. Examples of the article include furniture, electrical appliances, plumbing articles, building materials, fittings, clothing, bedding, etc. More specifically, for example, a table, chair, sink, toilet seat, doorknob, faucet, switch, remote control device (such as a remote controller for various devices), computer input devices (such as a keyboard, mouse, etc.), wallpaper, fabric ceiling, curtains, carpets, etc. can be mentioned. Also, as the article, for example, an elevator switch installed in a building, an escalator handrail installed in a building, a ticket vending machine in a transportation facility, a hanging strap in a vehicle, a shopping basket or cart frequently used in a commercial facility, etc. can be mentioned. In the present invention, amylase can be detected on the surface of these articles which are the detection objects.

[0026] In the present invention, the environment may be an area to be subjected to sanitation treatment (hereinafter also referred to as a target area). Hereinafter, the term "target area" may mean the environment. The target area may be selected, for example, from buildings such as the above-mentioned commercial facilities, accommodation facilities, business facilities, etc., and transportation public facilities. A location for detecting amylase is selected from those target areas. The location for detecting amylase can be appropriately selected in consideration of the use, scale, etc. of the target area. In the present invention, the area to be subjected to sanitation treatment in the environment is determined, and amylase can be detected in that target area. That is, in the present invention, the area to be subjected to sanitation treatment in the environment is determined, and amylase can be detected in the detection object in that target area.

[0027] In the present invention, it is preferable to detect amylase on the surface of the object to be detected in the target area, and further measure the amount of amylase if necessary. The amylase may be amylase derived from humans.

[0028] Sampling of a sample for detecting amylase from the surface of the object to be detected in the target area can be performed by bringing an appropriate sampling means, for example, absorbent cotton, non-woven fabric, sponge, etc. into contact with the surface of the object to be detected. In the present invention, for detecting amylase from the surface of the object to be detected, it is preferable to use a sampling means having a support portion and a sampling portion for a sample provided on the support. In the present invention, it is preferable to use a swab or a cotton swab as the sampling means. These may be for medical use or for specimen collection. The sampling portion can take various shapes such as spherical and longitudinal. Further, the sampling portion may be composed of, for example, fibers, porous materials, etc. Specific examples of the fibers include natural fibers such as cotton, synthetic fibers, and blended fibers thereof. Examples of the porous material include sponges. The sampling portion preferably has liquid absorbency. The sampling portion is preferably kept in a wet state with an appropriate liquid. That is, it is preferable that the sampling portion holds the liquid. The amount of the liquid held in the sampling portion is dripping isAn amount that does not occur is preferred. The dry mass of the collection part, for example, a fibrous material or a porous material, is preferably, for example, 10 mg or more and 1000 mg or less. The liquid for supporting on the collection part may be, for example, water. Further, the liquid may be an aqueous solution containing a surfactant, physiological saline, a buffer solution, etc., or may be a composition containing a combination of components formulated therein. The surfactant may be selected from those that do not affect the detection of amylase. Examples of the surfactant include nonionic surfactants. As the nonionic surfactant, for example, it has an alkyl group having 6 to 22 carbon atoms, the average added mole number of oxypropylene groups is 0 or more and 5 or less on average, and the average added mole number of oxyethylene groups is 5 or more and 50 or less. Examples thereof include polyoxyalkylene alkyl ethers and polyoxyethylene alkyl phenyl ethers, and polyoxyalkylene adducts of esters of polyhydric alcohols such as glycerin, pentaerythritol, and sorbitol and fatty acids. As the nonionic surfactant, those commercially available under trade names such as Triton X-100, Nonidet P-40 (NP-40), Tween20, and Tween80 can be used. The concentration of the surfactant in the liquid may be determined within a range that does not affect the detection of amylase. The concentration of the surfactant in the liquid may be, for example, 2% by mass or less, further 1% by mass or less, further 0.5% by mass or less, further 0.1% by mass or less, further 0.05% by mass or less, further 0.01% by mass or less, further 0.005% by mass or less.

[0029] In order to obtain common information by aligning sampling conditions at the sampling locations, for example, in units of specific sites, rooms, buildings, or regions, it is preferable to use sampling means in which substantially the same amount of liquid is carried on the same type of sampling part. That is, usually, since a plurality of sampling means are used for sampling a sample, each has the same type of sampling part, and it is preferable that the sampling part carries the same amount of liquid at the initial stage before contacting the surface of the object to be detected. For this purpose, a plurality of supports in which the sampling part is wetted with a predetermined amount of liquid and sealed with an aluminum package or a highly airtight container may be prepared. Also, when separating the sampling part and the initial wetting liquid, a method of putting a certain amount of liquid into a microtube, test tube, etc. in advance, sealing it, opening it before use, contacting it with the sampling part, and sucking the liquid so as not to absorb more than a specific amount can also be considered. The amount of liquid carried by the sampling part is preferably 10 mg or more, more preferably 30 mg or more, still more preferably 90 mg or more, and preferably 1500 mg or less, 1000 mg or less, 500 mg or less in the case of a cotton swab. Although the amount of liquid sucked can vary depending on the inspection area and the nature of the sampling part, it is preferably 200 mg or less, more preferably 100 mg, which is generally easy to use. The ratio of the liquid to the sampling part (for example, the cotton of a cotton swab) is preferably 0.5 or more, more preferably 1 or more, still more preferably 2 or more, even more preferably 3 or more, and preferably 5 or less, more preferably 4 or less by mass ratio.

[0030] In addition, specific examples of the support include those made of wood or resin. When the amylase is not extracted immediately from the collected cotton swab, for example, when it is taken back to a laboratory separate from the collection location for extraction, it becomes difficult to extract the amylase. Therefore, a resin-made support is preferred. This is presumably because in the case of a wooden support, the liquid gradually migrates from the collection part to the support part. Also, when the amylase is not extracted immediately from the collected cotton swab, it is preferably stored at a temperature of 10°C or lower, more preferably 5°C or lower, and preferably 0°C or higher, more preferably 2°C or higher. It is preferable to transport and store the collection means within this temperature range. At this time, it is preferable to store the cotton swab sealed in a plastic container with a dip, a sample bottle, an aluminum package, or a highly airtight container. Also, when the amylase is not detected immediately from the extract collected from the collection part, it is preferably stored at a low temperature, preferably 10°C or lower, more preferably 5°C or lower, and preferably 0°C or higher, more preferably 2°C or higher. It is preferable to transport and store the extract within this temperature range.

[0031] In addition, when using the above-mentioned collection means, when the liquid carried on the collection part is 90 mg or more and 500 mg or less, the inspection area targeted by one collection part is preferably 100 mm 2 or more, more preferably 2000 mm 2 or more. From the perspective of maintaining the wettability of the collection part and the wiping efficiency of amylase, it is preferably 30000 mm 2 or less, more preferably 10000 mm 2 or less. When sampling a sample using the above-mentioned collection means, when the inspection surface is large, a square corresponding to the above area is assumed as the target surface, and on that surface, the number of contacts is determined to be 10 or more and 20 or less in each of the vertical, horizontal, and diagonal (two diagonals) directions for sampling. That is, on one surface, it is preferably sampled a total of the number of contacts in one direction × 4 times. When the inspection surface is curved or rectangular, the sample may be sampled by setting a range close to the determined area.

[0032] When using the above-described sampling means, the extraction of amylase from the sampling part after contacting the surface of the object to be detected may be performed by bringing the sampling part into contact with an extraction liquid, for example, by immersing it in the extraction liquid contained in an appropriate container. The amount of the extraction liquid varies depending on the size of the sampling part and the like. For example, in the case of a cotton swab, the amount of the extraction liquid is preferably 30 mg or more, more preferably 50 mg or more, and preferably 10,000 mg or less, more preferably 5,000 mg or less, and even more preferably 1,000 mg or less. Preferably, the amount of the extraction liquid is 0.1 times or more, and further 0.5 times or more, and 10 times or less, further 5 times or less, and even further 2 times or less, based on mass, of the amount of the liquid impregnated in the sampling part. In the case of immersion, the immersion time may be, for example, 10 seconds or more, further 1 minute or more, 5 minutes or more, 10 minutes or more, 30 minutes or more, 1 hour or more, 2 hours or more, and less than 48 hours, further 24 hours, 12 hours, and less than 6 hours. In the case of immersion, the immersion temperature can be a temperature at which the amylase does not denature, for example, room temperature (as an example, 10°C or more and 30°C or less). The same composition as the above-described liquid may be used as the extraction liquid. While the sampling part is immersed in the extraction liquid, it may be left standing, or an external force load may be applied, for example, application of ultrasonic waves, stirring by a stirring device such as a vortex mixer, manual shaking, pressing with a rod or the like, pressing against the container wall surface, etc. Also, when a small amount of the extraction liquid is used, the liquid may be separated from the sampling part by centrifugation or pressing. Amylase may be detected from the extraction liquid obtained by bringing the sampling part into contact by such a method, for example, by a method using an antigen-antibody reaction and / or an enzyme reaction described later. Note that for the sampling, extraction, etc. of amylase, a commercially available kit suitable for this can be used, and in that case, the sampling of amylase may be performed according to the method described in the kit. Also, the above-described liquid, the extraction liquid, etc. may be those attached to the kit.

[0033] As one aspect of the present invention, there is provided a method for determining a hygienic treatment location, which includes detecting amylase on the surface of an object to be detected in the environment, and further an object to be detected in the living environment, and determining a location for performing hygienic treatment based on the detection result. As another aspect of the present invention, there is provided a method for determining a location for sanitation treatment, which involves detecting amylase on the surface of a detection target in the environment, particularly in the living environment, and determining the location for sanitation treatment based on the detection result. The method includes collecting a sample from the surface of the detection target using sampling means having a support portion and a sample collection portion provided on the support, preferably using a swab and / or a cotton swab, and detecting amylase from the sample.

[0034] In the present invention, amylase can be detected at different locations in the target area, and the location where amylase is detected relatively more can be set as the location for sanitation treatment.

[0035] In the present invention, amylase is detected in the target area, and based on the detection result, conditions for sanitation treatment, such as the type of treatment, the type and amount of chemicals used in the treatment, the number of times of treatment, etc., are determined. The conditions can be adopted as they are or with modifications, which are the conditions usually used by those skilled in the art for each treatment.

[0036] It is assumed that locations with a high detection amount of amylase are locations where amylase or components containing amylase, such as saliva, are likely to adhere in various forms. Such locations are likely to be contaminated by, for example, saliva, viruses and / or bacteria taken into saliva even after being cleaned once. When determining the location and conditions for sanitation treatment based on the detection result of amylase, this can be taken into consideration.

[0037] In the present invention, amylase is detected at different locations in the target area, and the conditions for sanitation treatment can be determined from the detection frequency and detection amount of amylase. Specifically, amylase is detected at different locations in the target area, and the conditions for sanitation treatment can be determined from the amylase positive rate and average amylase amount calculated from the detection result. Here, the amylase positive rate is the ratio of the number of locations where amylase is detected to the number of locations where amylase detection is performed, and is calculated by the following formula. Amylase positive rate (%) = B / A × 100 A: The number of locations where amylase detection is performed B: The number of locations where amylase is detected Also, the average amylase amount is the average value of the amylase amount at the locations where amylase detection is performed. In this case, the amylase amount may be based on a result indicating the detection amount relatively, for example, the detection intensity of a reagent.

[0038] The amylase positive rate is the probability that the target area is contaminated, and the average amylase amount may be interpreted as the amount of contaminants adhering to the target area on average. In the present invention, based on the detection result of amylase, the possibility of contamination by a virus and / or bacteria can be determined. In this case, the higher the numerical values of the amylase positive rate and the average amylase amount at a location, the higher the infection risk when a person contacts the surface of the target area. In the present invention, it is preferable to set criteria for determining the possibility of contamination by a virus and / or bacteria from the amylase positive rate and the average amylase amount, and determine the infection risk based on the criteria. Specifically, the amylase positive rate and the average amylase amount may be provided with criteria of two or more levels, preferably three or more levels, and ten levels or less, preferably five levels or less, respectively, to determine the infection risk and judge the priority order of performing sanitation treatment, etc. For example, the amylase positive rate and the average amylase amount may be associated as shown in Table 1 described later to perform risk determination and judge the priority order of performing sanitation treatment, etc. Note that the risk determination criteria in Table 1 are an example, and the magnitudes of the effects of the amylase positive rate and the average amylase amount on the infection risk may be determined based on criteria different from those in Table 1.

[0039] When performing amylase detection for different locations in the target area in the present invention, for example, 1 m 2Measurements can be taken at a ratio of one or more locations, further two or more locations, further three or more locations, and up to 16 locations, further up to 9 locations, further up to 6 locations. The different locations may be selected on the same detection target or on different detection targets.

[0040] In the present invention, the detection of amylase can be carried out by a method using an antigen-antibody reaction and / or an enzyme reaction. The detection of amylase in the target area may be carried out directly on the surface of the detection target, for example, or on a sample collected from the surface of the detection target. In the present invention, it is preferable to carry out the detection of amylase by a method using an antigen-antibody reaction. In the present invention, for example, it is preferable to carry out the detection by a method using an antigen-antibody reaction capable of specifically detecting human salivary amylase. Examples of methods using an antigen-antibody reaction include immunochromatography, enzyme immunoassay (EIA method, ELISA method), immunoprecipitation, immunoblotting, and the like. A commercially available product for measuring the amount of amylase using an antigen-antibody reaction may also be used.

[0041] In the present invention, the sanitation treatment may be a treatment applied to the target area where the detection of amylase has been carried out. That is, in the present invention, for example, when the detection of amylase is carried out on the surface of the detection target, the sanitation treatment may be a sanitation treatment applied to the detection target and / or the environment including the detection target.

[0042] In the present invention, the sanitation treatment may include a treatment for improving the sanitation state of the target area, a treatment for maintaining the sanitation state of the target area, and the like. Generally, the sanitation state of the living environment is improved and / or maintained by keeping it clean. In the present invention, for example, the sanitation treatment may include one or more treatments selected from purification, anti-bacterial adhesion treatment, and anti-viral adhesion treatment. More specifically, the sanitation treatment may include, for example, one or more treatments selected from washing, bleaching, disinfection, sterilization, decontamination, antibacterial treatment, antiviral treatment, and virus removal. Each treatment can be carried out by a known method.

[0043] In the present invention, the sanitation treatment may be a treatment for reducing the disadvantages caused by viruses and / or bacteria. That is, the viruses and / or bacteria targeted by the present invention may be harmful viruses and / or harmful bacteria. The harm includes at least harm to humans. The harmful virus may be a non-enveloped virus or an enveloped virus, particularly an enveloped virus. The treatment for reducing the disadvantages caused by viruses and / or bacteria can be specifically selected from the above-mentioned treatments. The disadvantages caused by viruses and / or bacteria are typically infectious diseases.

[0044] In the present invention, the sanitation treatment may be a treatment using a sanitation treatment agent (hereinafter referred to as the sanitation treatment agent) for at least any one selected from washing, sterilization, and virus killing. The sanitation treatment agent can contain, for example, one or more selected from (a) a surfactant, (b) an oxidizing bleaching agent, and (c) a bactericide. The sanitation treatment agent can contain water. The sanitation treatment agent can be either liquid or solid, but liquid is preferred, and a liquid containing water is more preferred.

[0045] (a) The surfactant includes one or more selected from an anionic surfactant, a nonionic surfactant, a cationic surfactant, and an amphoteric surfactant. (a) Examples of the surfactant include one or more selected from linear alkylbenzene sulfonic acid and its salts, alkyl sulfate esters and their salts, polyoxyethylene alkyl ether sulfates and their salts, fatty acids and their salts, polyoxyethylene alkyl ethers, alkyl glycosides, alkylamine oxides, quaternary ammonium salts, and alkylamine ethylene oxide adducts. The carbon number of the linear alkyl group of the linear alkylbenzene sulfonic acid may be 8 or more and 20 or less. The carbon number of the alkyl group of the alkyl sulfate ester may be 8 or more and 20 or less. The average number of moles of ethylene oxide added to polyoxyethylene alkyl ether may be 1 or more and 30 or less, and the number of carbon atoms in the alkyl group may be 8 or more and 20 or less. The number of carbon atoms in the fatty acid may be 8 or more and 20 or less. Examples of the salt include alkali metal salts such as sodium salt and potassium salt. The average number of moles of ethylene oxide added to polyoxyethylene alkyl ether may be 1 or more and 30 or less, and the number of carbon atoms in the alkyl group may be 8 or more and 20 or less. The number of carbon atoms in the alkyl glucoside may be 8 or more and 20 or less. The number of carbon atoms in the alkylamine oxide may be 8 or more and 20 or less. Examples of the quaternary ammonium salt include benzalkonium chloride, benzethonium chloride, and dialkyl (8 or more and 20 or less carbon atoms) dimethylammonium chloride. The number of carbon atoms in the alkylamine ethylene oxide adduct may be 8 or more and 20 or less, the average number of moles of ethylene oxide added may be 1 or more and 30 or less, and the amine may be primary, secondary, or tertiary. Examples of the alkylamine ethylene oxide adduct include laurylamine ethylene oxide adduct. Although it depends on the type of sanitation treatment, the sanitation treatment agent can contain (a) a surfactant, for example, 0.0001% by mass or more, further 0.001% by mass or more, further 0.01% by mass, further 0.1% by mass, and 20% by mass or less, further 10% by mass or less, further 5% by mass or less.

[0046] (b) Examples of the oxidizing bleaching agent include hypochlorite, dichloroisocyanurate, hydrogen peroxide, sodium percarbonate, and peracetic acid, with hypochlorite being preferred. Examples of the salt include alkali metal salts such as sodium salt and potassium salt. Although it depends on the type of sanitation treatment, the sanitation treatment agent can contain (b) an oxidizing bleaching agent, for example, 0.0001% by mass or more, further 0.001% by mass or more, further 0.01% by mass, further 0.1% by mass, further 1% by mass, and 10% by mass or less, further 7.5% by mass or less, further 5% by mass or less.

[0047] (c) Examples of the bactericide include one or more selected from bactericidal alcohols and bactericidal organic acids.

[0048] Examples of the bactericidal alcohol include one or more selected from ethanol and propanol. Depending on the type of the sanitation treatment, the sanitation treatment agent may contain the bactericidal alcohol, for example, at 0.1% by mass or more, further 1% by mass or more, further 10% by mass, further 40% by mass, and 95% by mass or less, further 90% by mass or less, further 85% by mass or less.

[0049] Examples of the bactericidal organic acid include lactic acid or a salt thereof. Examples of the salt include alkali metal salts such as sodium salt and potassium salt. Depending on the type of the sanitation treatment, the sanitation treatment agent may contain the bactericidal organic acid, for example, at 0.01% by mass or more, further 0.1% by mass or more, further 0.4% by mass, and 7% by mass or less, further 5% by mass or less, further 3% by mass or less.

[0050] (c) The bactericide may be one or more selected from ethanol, propanol, lactic acid, and a salt of lactic acid.

[0051] Preferably, the sanitation treatment agent contains two or more selected from (a) a surfactant, (b) an oxidative bleaching agent, and (c) a bactericide.

[0052] In the present invention, the sanitation treatment may include contact of the sanitation treatment agent with the treatment site and wiping of the treatment site with a flexible liquid-absorbing material that has been brought into contact with the sanitation treatment agent. In the present invention, the contact of the sanitation treatment agent with the treatment site can be performed by at least one of contact of a flexible liquid-absorbing material impregnated with the sanitation treatment agent with the treatment site and spraying of the sanitation treatment agent onto the treatment site. For example, when wiping the treatment site with a flexible liquid-absorbing material impregnated with the sanitation treatment agent, it is possible to wipe the treatment site while bringing the sanitation treatment agent into contact with the treatment site. Spraying can be performed using, for example, a trigger pump. Examples of the flexible liquid-absorbing material include non-woven fabric, woven fabric, and sponge.

[0053] The present invention provides a method for determining a sanitation treatment method, which detects amylase in the environment and determines the location for performing sanitation treatment and the conditions for sanitation treatment based on the detection results. In this method, the method for determining the location for performing sanitation treatment may be the method for determining the sanitation treatment location of the present invention. Also, in this method, the method for determining the conditions for sanitation treatment may be the method for determining the method for determining the sanitation treatment conditions of the present invention. Based on the location for performing sanitation treatment and the sanitation treatment conditions determined in this way, a sanitation treatment method is determined.

[0054] In the present invention, the detection of amylase is carried out to determine the location for performing sanitation treatment and / or the conditions for sanitation treatment. In the present invention, the detection of amylase may be carried out every time sanitation treatment is performed, but it is not necessary to perform it every time. Also, for example, if the information on the type, degree, frequency, etc. of the sanitation treatment for each location is manualized based on the information on the location and / or conditions determined by the present invention, then hereafter, as long as the use of that location does not change significantly, highly hygienic treatment can be continuously implemented without necessarily detecting amylase. Also, once the location for performing sanitation treatment and / or the conditions for sanitation treatment are determined, the detection of amylase may be carried out periodically or aperiodically to confirm the quality and effect of the determined sanitation treatment method.

[0055] Using the determination method of the present invention, for example, it is possible to assist in sanitation treatment for the above-mentioned buildings, public transportation periods, and their equipment. That is, by providing the information on the sanitation treatment method determined by the present invention to the person who performs the sanitation treatment, the predetermined sanitation treatment can be surely and efficiently executed.

[0056] As a more specific aspect of the present invention, there is provided a method for determining a sanitation treatment method, which includes detecting amylase in a target area, determining a location where amylase is detected as a location potentially contaminated by a virus and / or bacteria, and determining conditions for a sanitation treatment to be applied to the determined location based on the detection result of amylase. In this method, the matters described in each of the determination methods of the present invention described above can be appropriately applied. As another more specific aspect of the present invention, there is provided a method for determining a sanitation treatment method, which includes detecting amylase in a building, for example, indoors and / or outdoors, determining a location where amylase is detected as a location potentially contaminated by a virus and / or bacteria, and determining conditions for a sanitation treatment to be applied to the determined location based on the detection result of amylase. In this method, the matters described in each of the determination methods of the present invention described above can be appropriately applied.

[0057] The present invention provides a sanitation treatment method for determining at least one of a location for performing a sanitation treatment and conditions for the sanitation treatment by the determination method of the present invention, and performing a sanitation treatment based on the determination. That is, the sanitation treatment method of the present invention is (1) determining a location for performing a sanitation treatment by the determination method of the present invention, and performing a sanitation treatment on the determined location; (2) determining conditions for a sanitation treatment by the determination method of the present invention, and performing a sanitation treatment under the determined conditions; or (3) determining a location for performing a sanitation treatment and conditions for the sanitation treatment by the determination method of the present invention, and performing a sanitation treatment on the determined location under the determined conditions. This is the sanitation treatment method. In the sanitation treatment method of the present invention, the matters described in the determination method of the present invention can be appropriately applied. It is also preferable that the sanitation treatment of the sanitation treatment method of the present invention is performed using a sanitation treatment agent. Specific examples and preferred examples of the sanitation treatment and the sanitation treatment agent can also be those described in the determination method of the present invention.

[0058] The present invention provides a method for detecting amylase in an environment and, based on the detection result, estimating a location where a harmful virus and / or harmful bacterium is likely to be present. Further, the present invention provides a method for detecting amylase in an environment, estimating a location where a harmful virus and / or harmful bacterium is likely to be present based on the detection result, and estimating an infection route, that is, an infection route caused by the harmful virus and / or harmful bacterium. The infection route here may be an infection route to humans. Also, the infection route may be either a route for an already-occurred infection or a route for an expected future infection. Note that the estimation method according to the present invention also includes estimating a location or infection route where a harmful virus and / or harmful bacterium is likely to be present through empirical knowledge or artificial intelligence (AI) etc. as the accumulation of past data even in a first environment. Also, amylase detection may be performed after the estimation. Matters described in each determination method of the present invention described above can be appropriately applied to these methods. Also, in these methods, the harmful virus may be a non-enveloped virus or an enveloped virus, particularly an enveloped virus.

[0059] Examples of the harmful bacterium include Streptococcus pneumoniae etc. Examples of the non-enveloped virus include adenovirus, rhinovirus, and norovirus etc. Examples of the enveloped virus include SARS-CoV-2, influenza, SARS, MARS, and RS virus etc.

[0060] The present invention provides a sanitation treatment kit including means for detecting amylase in a target area and a drug used for treating a sanitation treatment site determined based on a detection result of amylase obtained by the means. Examples of the means for measuring the amount of amylase include an immunochromatographic method, an enzyme immunoassay (EIA method, ELISA method), an immunoprecipitation method, immunoblotting, and enzyme activity measurement. Examples of the drug include a detergent, a bleaching agent, a disinfectant, a virucide, an antiviral agent, a bactericide, and an antibacterial agent.

[0061] The present invention provides a method for displaying a contamination status at a candidate site for sanitation treatment, (a) A step of identifying one or more locations in the environment, the step including the following steps (i) to (iii): (i) A step of generating position data capable of identifying the installation location of an actual object to identify the one or more locations within a defined area, (ii) A step of recording the position data of the one or more locations in the environment, and (iii) A step of separately recording the actual objects linked to the position data of the one or more locations in the environment by position and part, (b) A step of recording a detection result of amylase detected from one or more locations in the environment, (c) A step of recording the generated detection result according to the separately recorded position and part of the actual object recorded in (iii) of (a), and (d) A step of displaying the recording in (c) together with the position data of the one or more locations in the environment. A method for displaying a contamination status at a candidate site for sanitation treatment is provided. The method for displaying the contamination status of the present invention is preferably performed by an information processing device (such as a PC or a smartphone). That is, it is preferable that the recording of each data in each step is performed by the information processing device. The outline of the procedure of the method for displaying the contamination status at the candidate locations for the sanitation treatment of the present invention is shown in FIG. 1. In FIG. 1, ST is an abbreviation for step. In FIG. 1, step (a) (not shown) is executed by ST(a)-(i), ST(a)-(ii), and ST(a)-(iii).

[0062] Step (a) is a step of identifying one or more locations in the environment, and appropriately selecting locations that are candidates for sanitation treatment in the environment. For example, as the location, indoor articles, and further articles in the toilet can be selected. Step (i) [ST(a)-(i) in FIG. 1] is a step of generating position data that can identify the installation locations of real objects in order to identify the one or more locations within the defined area. The area may be, for example, a predetermined area indoors. The real objects include, for example, indoor articles, and further articles in the toilet. The position data is, for example, data indicating the indoor positions of those articles. Step (ii) [ST(a)-(ii) in FIG. 1] is a step of recording the position data of the one or more locations in the environment. Step (iii) [ST(a)-(iii) in FIG. 1] is a step of separately recording the real objects linked to the position data of the one or more locations in the environment by position and part. For example, when there are multiple articles with the same attribute indoors, they are separated and individual information is created and recorded. Specifically, when there are two chairs indoors, they are sorted as chair A and chair B, and their respective position data is recorded. Further, when each chair has two armrests, the position data can also be created by subdividing them as armrest A1 and armrest A2. Of course, this processing method can be applied to other real objects (such as articles).

[0063] Step (b) [ST(b) in FIG. 1] is a step of recording the detection results of amylase detected from one or more locations within the environment. The detection of amylase is performed at the positions and parts of the actual objects separated in step (iii) of step (a). The detection results can be obtained, for example, by the detection method employed in the method for determining the sanitary treatment location of the present invention.

[0064] Step (c) [ST(c) in FIG. 1] is a step of recording the generated detection results in association with the separated positions and parts of the actual objects recorded in step (iii) of step (a). By this step (c), the detection results in the actual objects at a predetermined position and the detection results at a predetermined part of the actual objects at a predetermined position are recorded, and the positions and parts of the actual objects are associated with the detection results.

[0065] Step (d) [ST(d) in FIG. 1] is a step of displaying the recording in step (c) together with the position data of the one or more locations within the environment. The display is performed, for example, on an information display terminal such as an external monitor for a personal computer or a smartphone screen.

[0066] For example, in FIG. 1, taking ST(a)-(i) to ST(d) as one unit, ST(a)-(i) to ST(d) can be repeatedly performed until a predetermined number of times is reached, and it can also be terminated after reaching the predetermined number of times. Also, for example, in FIG. 1, taking ST(a)-(i) to ST(d) as one unit, ST(a)-(i) to ST(d) can be repeatedly performed at a predetermined interval, and it can also be terminated after reaching a predetermined time.

[0067] According to the present invention, there is provided a method for displaying a sanitation treatment location, which determines the sanitation treatment location based on the contamination status at the candidate locations for sanitation treatment displayed by the display method of the present invention and displays it as the sanitation treatment location. The method for displaying the sanitation treatment location of the present invention is preferably performed by an information processing device (such as a PC or a smartphone). In the method for displaying the sanitation treatment location of the present invention, for one or more locations within the environment specified in step (a), the sanitation treatment location can be determined based on the data of a plurality of candidate locations for sanitation treatment obtained by changing the measurement time. For the determination of the sanitation treatment location, for example, AI can be utilized. That is, for example, data such as the detection results of amylase, the locations and articles where amylase detection was performed, and the occurrence status of past infections can be accumulated, and the sanitation treatment location can be determined by utilizing AI.

[0068] The method for displaying the candidate locations for sanitation treatment and / or the method for displaying the sanitation treatment location of the present invention can be incorporated and implemented in the method for determining the sanitation treatment location, the method for determining the sanitation treatment conditions, the method for determining the sanitation treatment method, and the estimation method of the present invention. For example, in the method for displaying the sanitation treatment location of the present invention, based on the contamination status in the method for displaying the contamination status of the present invention, the possibility of contamination by viruses and / or bacteria can be determined, and the sanitation treatment location can be determined from the determination result. The determination of the possibility of contamination may include estimating the possibility of contamination. In addition, the result displayed according to the present invention can be printed and output on a paper medium or the like as needed. Also, the electronic data of the displayed result can be stored in an appropriate storage medium. Further, the electronic data of the displayed result can be secondarily utilized using an information communication network (Internet), for example, for services such as creating an implementation plan for sanitation treatment and guiding the sanitation treatment procedure based on the implementation plan.

[0069] The embodiments of the present invention are exemplified below. In these embodiments, the matters described in the method for determining the sanitation treatment location, the method for determining the sanitation treatment conditions, the method for determining the sanitation treatment method, the sanitation treatment method, the sanitation treatment kit, the estimation method, the method for displaying the contamination status at the candidate locations for sanitation treatment, and the method for displaying the sanitation treatment location of the present invention can be appropriately applied.

[0070] <1> A method for determining a location for sanitation treatment, which detects amylase in an environment and determines the location for sanitation treatment based on the detection result.

[0071] <2> The determination method according to <1>, wherein amylase is detected at different locations in the environment, and the location where amylase is detected relatively more among the detected locations is set as the location for sanitation treatment.

[0072] <3> The determination method for the sanitation treatment location according to <1> or <2>, which determines the possibility of contamination by viruses and / or bacteria based on the detection result of amylase and determines the location for sanitation treatment from the determination result.

[0073] <4> A method for determining sanitation treatment conditions, which detects amylase in an environment and determines the sanitation treatment conditions based on the detection result.

[0074] <5> The determination method according to <4>, wherein amylase is detected at different locations in the environment, and the sanitation treatment conditions are determined from the detection frequency and detection amount of amylase.

[0075] <6> The determination method according to <4> or <5>, which determines the possibility of contamination by viruses and / or bacteria based on the detection result of amylase and determines the sanitation treatment conditions from the determination result.

[0076] <7> A method for determining a sanitation treatment method, which detects amylase in an environment and determines the location for sanitation treatment and the sanitation treatment conditions based on the detection result.

[0077] <8> The determination method according to <7>, wherein detection of amylase is performed at different locations in the environment, and a location where amylase detection is relatively high among the detected locations is set as a location for performing sanitation treatment.

[0078] <9> The determination method according to <7> or <8>, wherein detection of amylase is performed at different locations in the environment, and the sanitation treatment conditions are determined based on the detection frequency and detection amount of amylase.

[0079] <10> The determination method according to any one of <7> to <9>, wherein based on the detection result of amylase, the possibility of contamination by viruses and / or bacteria is determined, and the location for performing sanitation treatment and / or the sanitation treatment conditions are determined from the determination result.

[0080] <11> The determination method according to any one of <1> to <10>, wherein the sanitation treatment is a treatment for reducing the disadvantages caused by viruses and / or bacteria.

[0081] <12> The determination method according to any one of <1> to <10>, wherein the sanitation treatment includes one or more treatments selected from purification, anti-bacterial adhesion treatment, and anti-virus adhesion treatment.

[0082] <13> The determination method according to any one of <1> to <12>, wherein the detection of amylase is performed by a method using an antigen-antibody reaction and / or an enzyme reaction.

[0083] <14> The determination method according to any one of <1> to <13>, wherein the detection of amylase is performed by a method using an antigen-antibody reaction.

[0084] <15> The determination method according to any one of <1> to <14>, wherein the amylase is human-derived amylase.

[0085] <16> The determination method according to any one of <1> to <15>, wherein the environment is a living environment.

[0086] <17> The determination method according to any one of <1> to <16>, wherein the environment includes an object for detecting amylase (hereinafter referred to as a detection object), and amylase is detected on the surface of the detection object.

[0087] <18> The determination method according to <17>, wherein the detection object includes a detection object selected from a building, a building equipment, a public transportation vehicle, and a public transportation vehicle equipment.

[0088] <19> The determination method according to <18>, wherein the building is a building selected from a commercial facility, a lodging facility, an educational facility, a medical facility, a nursing facility, a business facility, and a food service facility, and the public transportation vehicle is a public transportation vehicle selected from a railway vehicle, a road vehicle, a ship, and an aircraft.

[0089] <20> The determination method according to any one of <17> to <19>, wherein the detection object includes a detection object selected from a table, a chair, a sink, a toilet seat, a doorknob, a faucet, a switch, a remote control device, and a computer input device.

[0090] <21> The determination method according to any one of <1> to <20>, wherein the sanitation treatment is a treatment using a sanitation treatment agent for at least any one selected from washing, sterilization, and virus killing.

[0091] <22> The determination method according to <21>, wherein the sanitation treatment agent contains one or more selected from (a) a surfactant, (b) an oxidative bleaching agent, and (c) a bactericide.

[0092] <23> The determination method according to <21> or <22>, wherein the sanitation treatment agent contains two or more selected from (a) a surfactant, (b) an oxidative bleaching agent, and (c) a bactericide.

[0093] <24> (a) The surfactant is one or more selected from linear alkylbenzene sulfonic acid and its salts, fatty acids and their salts, polyoxyethylene alkyl ethers, alkyl glycosides, alkylamine oxides, quaternary ammonium salts, and alkylamine ethylene oxide adducts, and the determination method according to <22> or <23>.

[0094] <25> (b) The oxidative bleaching agent is hypochlorite, and the determination method according to any one of <22> to <24>.

[0095] <26> (c) The bactericide is one or more selected from bactericidal alcohols and bactericidal organic acids, and the determination method according to any one of <22> to <25>.

[0096] <27> (c) The bactericide is one or more selected from ethanol, propanol, lactic acid, and salts of lactic acid, and the determination method according to any one of <22> to <26>.

[0097] <28> The sanitation treatment includes contact of the sanitation treatment agent with the treatment site and wiping of the treatment site where the sanitation treatment agent is contacted with a flexible liquid-absorbing material, and the determination method according to any one of <21> to <27>.

[0098] <29> The contact of the sanitation treatment agent with the treatment site is performed by at least one of contact of a flexible liquid-absorbing material impregnated with the sanitation treatment agent with the treatment site and spraying of the sanitation treatment agent onto the treatment site, and the determination method according to <28>.

[0099] <30> A sanitation treatment method for determining at least one of the location for performing sanitation treatment and the conditions for sanitation treatment by the determination method according to any one of <1> to <29> and performing sanitation treatment based on the determination.

[0100] <31> The sanitation treatment method according to <30>, wherein the sanitation treatment is a treatment using a sanitation treatment agent for at least any one selected from cleaning, sterilization, and virus killing.

[0101] <32> The sanitation treatment method according to <31>, wherein the sanitation treatment agent contains one or more selected from (a) a surfactant, (b) an oxidizing bleaching agent, and (c) a bactericide.

[0102] <33> The sanitation treatment method according to <31> or <32>, wherein the sanitation treatment agent contains two or more selected from (a) a surfactant, (b) an oxidizing bleaching agent, and (c) a bactericide.

[0103] <34> The sanitation treatment method according to <32> or <33>, wherein (a) the surfactant is one or more selected from linear alkylbenzene sulfonic acid and its salts, fatty acids and their salts, polyoxyethylene alkyl ethers, alkyl glycosides, alkylamine oxides, quaternary ammonium salts, and alkylamine ethylene oxide adducts.

[0104] <35> The sanitation treatment method according to any one of <32> to <34>, wherein (b) the oxidizing bleaching agent is a hypochlorite.

[0105] <36> The sanitation treatment method according to any one of <32> to <35>, wherein (c) the bactericide is one or more selected from bactericidal alcohols and bactericidal organic acids.

[0106] <37> The sanitation treatment method according to any one of <32> to <36>, wherein (c) the bactericide is one or more selected from ethanol, propanol, lactic acid, and salts of lactic acid.

[0107] <38> A sanitation treatment kit comprising means for detecting amylase in an environment and a drug used for treating a sanitation treatment site determined based on the detection result of amylase obtained by the means.

[0108] <39> A method for estimation, which comprises detecting amylase in an environment and estimating a location highly likely to have a harmful virus and / or harmful bacteria based on the detection result.

[0109] <40> A method for estimation, which comprises detecting amylase in an environment, estimating a location highly likely to have a harmful virus and / or harmful bacteria based on the detection result, and estimating an infection route.

[0110] <41> The method for estimation according to <39> or <40>, wherein the harmful virus is an enveloped virus.

[0111] <42> A method for displaying a contamination status at a candidate site for sanitation treatment, comprising: (a) A step of identifying one or more locations in the environment, the step including the following steps (i) to (iii): (i) A step of generating position data capable of identifying the installation location of an actual object to identify the one or more locations within a defined area; (ii) A step of recording the position data of the one or more locations in the environment; and (iii) A step of separately recording the actual objects linked to the position data of the one or more locations in the environment by position and part; (b) A step of recording the detection result of amylase detected from one or more locations in the environment; (c) A step of recording the generated detection result according to the separately recorded positions and parts of the actual objects recorded in (iii) of (a); and (d) A step of displaying the recording in (c) together with the position data of the one or more locations in the environment. A method for displaying a contamination status at a candidate site for sanitation treatment, comprising the above steps.

[0112] <43> A method for displaying a sanitation treatment location, which determines a sanitation treatment location based on the contamination status at a candidate location for sanitation treatment displayed by the display method of <42> and displays it as the sanitation treatment location.

[0113] <44> The method for displaying a sanitation treatment location according to <43>, which determines the possibility of contamination by viruses and / or bacteria based on the contamination status and determines the sanitation treatment location from the determination result.

Example

[0114] <Example 1 and Comparative Example 1> (1) Example 1 The contamination risk was determined for a commercial facility (shopping center). The commercial facility was divided into three areas: (1) sales area, (2) dining area, and (3) rest area. Detection of amylase was performed at the inspection locations in each area. From the detection results, the average amylase amount and amylase positive rate were obtained for each area, and the contamination risk level was determined based on Table 1 below. The results are shown in Table 2.

[0115] At each inspection location, a 25 cm 2 area was wiped with a clean cotton swab. Detection of amylase from the wiped cotton swab was performed using an immunochromatography kit (Independent Forensics, RSID (registered trademark) SALIVA [model number: 0100]). If amylase was not detected at the target inspection location, it was considered negative "-", and if detected, it was considered positive "+". In the case of a positive result, it was visually evaluated in three levels according to the intensity of the test line band and used as the amylase amount. When the band was barely confirmed, it was +1, when it could be clearly confirmed, it was +2, and when it could be confirmed darkly, it was +3. It can be judged that the amount of amylase attached was large as the value of + increased. In addition, when measuring an amylase solution with a known concentration, the amylase amount corresponding to each determination result was 25 cm 2Per area of the region, "-" was less than 10 ng, "+1" was 10 ng or more and less than 25 ng, "+2" was 25 ng or more and less than 60 ng, and "+3" was 60 ng or more.

[0116] Note that the above inspection method can be carried out manually, and a kit suitable for the manualized content, for example, a kit suitable for implementation under the following conditions (hereinafter referred to as Manual Example 1), can be used. Wetting solution: 0.1 ml of an aqueous solution with a pH of 7 containing 0.05% Tween 80 in distilled water, sealed in a 0.5 ml microtube Sampling part: A cotton swab with a weight of 30 mg (excluding the indicating part) wrapped around one end and a length of 75 mm Extract: 0.1 ml of an aqueous solution with a pH of 7 containing 0.05% Tween 80 in distilled water, sealed in a squeezable tube Sampling method: When in use, put the cotton swab into the microtube to absorb the wetting solution.

[0117] When following the procedure under the above conditions, select a predetermined area, for example, an area of 50 mm × 50 mm, from the surface of the inspection target, for example, the surface of the target article, and bring the wetted sampling part into contact with it vertically, horizontally, and diagonally right and left (the order of vertical, horizontal, and diagonal does not matter). It is preferable to make contact so that the surface of the cotton swab touches evenly, for example, by interpreting the cotton swab as a quadrangular prism and distributing each direction to each surface, etc., but it is sufficient to make contact so that the sampling part does not become biased and run out of liquid. The carrier part of the sampled cotton swab etc. may be directly placed in a suitable container, for example, a plastic container with a zip or a sample bottle, and the container may contain the extract. Extract the amylase (obtain the liquid containing amylase) and detect and quantify the amylase from the carrier part of the sampled cotton swab etc. by the following method. After sampling, the sample may be moved to another location and the extraction operation may be carried out in the brought-back workroom. Extraction method: Immerse the sampling part of the cotton swab in the extract and knead the cotton ball from above the tube. Detection method: For example, in a commercially available immunochromatography kit (IndependentForensics, RSID (registered trademark) SALIVA [model number: 0100]), 0.08 mL of the attached Running buffer and 0.02 mL of the extract are mixed and dropped to detect amylase. Quantification method: By reducing the error of the above sampling method, amylase can also be quantified from the shade of the band at the detection site. Serial dilutions of an amylase solution with a known concentration can be measured, and the intensity of the band can be visually determined using a color scale, or quantification can also be performed using a commercially available immunochromatography reader. Note that the above sampling method may be set so that it is easy to process with a commercially available amylase measurement kit.

[0118] (2) Comparative Example 1 At the inspection locations in each area of the same commercial facility as in Example 1, the amount of ATP (RLU) was measured. The average value of the ATP amount was determined for each area from the measurement results. The results are shown in Table 2. The amount of ATP was measured using the LuciPac Pen manufactured by Kikkoman Biochemifa by wiping about 25 cm 2 of the target location and using the company's Lumitester Smart according to the method in the instruction manual attached to the product.

[0119]

Table 1

[0120]

Table 2

[0121] The average ATP amount in Comparative Example 1 contains not only viruses and / or bacteria but also other organic substances such as dirt. On the other hand, as shown in Reference Example 3 described later, it is suggested that there is a correlation between the amount of amylase and the amount of virus. Therefore, it can be judged that the contamination risk level based on the amylase detection result in Example 1 is more reliable than the contamination risk level based on the average ATP amount in Comparative Example 1. In Example 1, by measuring the amount of amylase, it was possible to estimate the location of amylase contamination, that is, the location where contamination by components containing amylase was highly likely. This could not be estimated from the amount of ATP. For example, in Comparative Example 1 of Table 2, the ATP amount of the floor (1) at 18,860 RLU was larger than the ATP amount of the register handle (1) at 17,973 RLU. However, in Example 1, the amount of amylase was larger in the former. Based on the results of Example 1, sanitation treatment for environmental surface contamination by amylase and components containing amylase can be carried out only at locations where amylase is detected, and the work can be made more efficient. Also, based on the results of Example 1, it is possible to estimate locations where harmful viruses and / or harmful bacteria are highly likely to exist, and further estimate the infection routes to humans. In Example 1, areas such as the sales area, dining area, and rest area were set, and the contamination risk level was evaluated based on the detection results of amylase in each area. As a result, the contamination risk levels of the dining area and the rest area were higher than that of the sales area. Therefore, it can be proposed to allocate greater sanitation treatment effort (materials and personnel) to the dining area and the rest area. When calculating the average ATP amount by area as in Comparative Example 1, it can be seen that the value of the sales area is larger than that of the rest area, which is a different trend from Example 1.

[0122] <Examples 2 and Comparative Example 2> (1) Example 2 The contamination risk was determined for the guest rooms of a hotel. For 8 guest rooms in the same hotel, at the inspection locations shown in Table 3, the amount of amylase was measured in the same manner as in Example 1, and the average value of the 8 rooms was obtained and used as the average amylase amount. Also, from the measurement results, the amylase positive rate was obtained, and the contamination risk level was determined based on Table 1 above. The results are shown in Table 3. Also, an example of displaying the contamination situation based on the results of Example 2 is schematically shown in FIG. 2. FIG. 2 schematically shows the overview of the hotel guest room and the inspection locations. The black circles attached to the inspection locations indicate the average amylase amount. The size of the black circles is correlated with the average amylase amount, meaning that the larger the size, the greater the average amylase amount.

[0123] (2) Comparative Example 2 At the inspection locations of 8 guest rooms in the same hotel as in Example 2, the ATP amount (RLU) was measured in the same manner as in Comparative Example 1. The average value of the ATP amount was determined for each inspection location from the measurement results. The results are shown in Table 3.

[0124]

Table 3

[0125] Based on the determination of the contamination risk level in Example 2, it was possible to propose the ranking of the places where meticulous sanitation treatment should be carried out or the places where sanitation treatment should be preferentially carried out. On the other hand, in Comparative Example 2, for example, at the edge of the washbasin sink where the contamination risk level was the highest in Example 2, the ATP amount was the lowest, indicating a different tendency from Example 2. Also, based on the results of Example 2, it is possible to estimate the locations where harmful viruses and / or harmful bacteria are likely to exist, and further estimate the infection routes to humans. The contamination situation in FIG. 2 can be displayed on the output device of an information processing apparatus (such as a PC or a smartphone). By displaying the contamination situation as shown in FIG. 2, the relationship between the inspection location and the contamination situation can be easily grasped visually. Furthermore, based on the contamination situation displayed in FIG. 2, the sanitation treatment location can be determined and displayed as the sanitation treatment location.

[0126] <Reference Example 1> Taking the table in the commercial facility where amylase was detected in Example 1 (inspection area: dining area, a table different from inspection locations (1), (2), and (3)) as the test object, the evaluation of the sanitation treatment effect was carried out. The table was wiped vertically and horizontally once each with a cloth moistened with tap water or a cloth moistened with a commercially available medical detergent (Medical Clenkeeper, Kao Corporation) diluted 200 times with tap water for sanitation treatment. The wiping with the cloth moistened with tap water and the cloth moistened with the commercially available medical detergent was carried out on different tables respectively. Before and after the sanitation treatment, the amount of amylase was measured in the same manner as in Example 1, and the amount of ATP was measured in the same manner as in Comparative Example 1. The results are shown in Table 4.

[0127]

Table 4

[0128] From the results in Table 4, it was determined that in the facilities of the said establishment, wiping with a medical cleaner was more effective in reducing the amount of amylase than wiping with water.

[0129] <Reference Example 2> (1) When sneezing indoors, (2) When three people were having a meal at a table indoors, and (3) When a web conference using a personal computer was held indoors, detection of amylase was carried out. (1) With one subject standing in a closed room with the window and door, without wearing a mask, sneezing was done 5 times, and amylase was detected directly below the subject, at points 50 cm, 1 m, and 2 m away from the subject. (2) Three subjects sat at a four-person table (without partitions), had a meal for 30 minutes while talking without wearing masks, and amylase was detected in the middle of the table and between two side-by-side subjects. (3) One subject sat at a desk and had a 2-hour and 30-minute web conference using a personal computer with a mask on, and amylase on the keyboard of the personal computer was detected. The detection of amylase was carried out in the same manner as in Example 1 in all cases. The locations where a large amount of amylase was detected are places where there is a high probability of saliva-derived stains. The results are shown in Table 5.

[0130]

Table 5

[0131] How saliva droplets scatter in the situations (1) to (3) above is predicted, for example, by simulations using the supercomputer "Fugaku". It was confirmed that the results in Table 5 correlate with such predicted results. Therefore, it is highly likely that the locations where amylase was detected in Examples 1 and 2 were contaminated by saliva droplets ejected from a person's mouth.

[0132] <Reference Example 3> The bacteriophage MS2 concentration was 10 10 PFU / mL, and the bacteriophage MS2 solution (10 12 PFU / mL) was mixed into saliva. 0.1 mL of this solution was measured and applied onto a PP (polypropylene) plate in a range of 4 cm × 4 cm, and left to stand in a safety cabinet for 1 hour to dry. This was taken as the initial attachment location. The following operations were performed from the initial attachment location. An arbitrary location of the initial attachment location was gently wiped once with a clean finger, and a new uncontaminated PP plate was gently wiped once with that finger part. This operation was repeated 5 times. The uncontaminated PP plate was wiped within a range of 4 cm × 4 cm. The wiped location on the new PP plate was taken as the propagation location (1). The same operation was performed on another new PP plate from the propagation location (1), and taken as the propagation location (2). Further, the same operation was performed on another new PP plate from the propagation location (2), and taken as the propagation location (3). The initial attachment location, propagation locations (1) to (3) were thoroughly wiped with a sterilized cotton swab moistened with sterilized water. The cotton swab was immersed in 0.2 mL of the extraction buffer (RSID (registered trademark) SALIVA Extraction Buffer) attached to the kit to prepare an extract of the wiped components. Of the extract, 0.1 mL was used for measuring the virus amount by the plaque assay described later, and the rest was used for measuring the amylase amount by an immunochromatography kit (Independent Forensics, RSID (registered trademark) SALIVA [model number: 0100]). The results are shown in Table 5.

[0133] [Method of Plaque Assay] LB agar medium (agar concentration: 1.5%) with calcium chloride added to a final concentration of 2.25 mM was autoclaved, and 15 mL was poured into a petri dish (Nissui Pharmaceutical Co., Ltd., Nissui sterilized shallow petri dish [model number: 06300]) and allowed to solidify at room temperature. LB agar medium (agar concentration 0.6%) with calcium chloride added to a final concentration of 2.25 mM was autoclaved and then maintained at about 40°C. 4 mL was taken and mixed with 0.9 mL of Escherichia coli solution (OD600 = 0.4) and 0.1 mL of the above extract, and the prepared LB agar medium was poured onto the solidified petri dish and layered. This was statically cultured at 37°C overnight, and the number of parts (plaques) where the virus was infected and lysed by Escherichia coli on the agar was counted as the virus amount (PFU / cm 2 ).

[0134]

Table 6

[0135] By the method of this reference example, the transmission of saliva by hand contact was confirmed. Therefore, it was suggested that the locations where amylase was detected in Examples 1 and 2 might be not only the locations where droplets adhered but also the locations of saliva transmission through contact with objects such as hands. Also, since it was confirmed in this reference example that the trends of the changes in the amount of virus and amylase due to transmission were similar, the locations where amylase was detected in the actual environment, regardless of the adhesion pattern of amylase (adhesion of droplets or transmission by contact), were considered to be places with a risk of contamination by components containing amylase, such as saliva, and thus viruses and / or bacteria in saliva. Moreover, this result suggests that the detection result of amylase can be used to estimate the infection route by viruses and / or bacteria.

[0136] <Reference Example 4> Multiple dried samples were prepared by applying 5 μL of saliva diluted 20-fold (collected from a 30-year-old male) onto polypropylene substrates. Amylase measurements were performed on each substrate according to the manual example 1 above. However, for the cotton swabs used as the sampling parts, (1) those with a wooden support (manufactured by Nippon Cotton Swab Co., Ltd., examiner-attached cotton swab CTB-15), and (2) those with a resin support (manufactured by Eiken Chemical Co., Ltd., γ Collect Swab R, TC2400) were used. Also, the quantification of amylase was divided into the following groups: (I) the group that quantifies immediately after collection, (II) the group that stores the cotton swab after collection at 4°C for 4 or 10 days and then quantifies, and (III) the group that stores the cotton swab after collection at 25°C for 4 or 10 days and then quantifies. The storage was carried out in the examiners attached to each cotton swab. The amount of amylase was quantified by the same method as in Example 1. The results are shown in Table 7.

[0137]

Table 7

[0138] From Table 7, it can be seen that when using a cotton swab with a wooden support, the detection sensitivity decreases when stored at 25°C. When the support is wooden, the detection intensity was maintained for 4 days by storing at 4°C, but the detection sensitivity decreased after 10 days. Therefore, it can be seen that low-temperature storage is preferable when the support is wooden. On the other hand, when using a cotton swab with a resin support, the detection sensitivity was maintained even at 25°C for 4 days. Therefore, when including the step of storing the cotton swab after collection, it can be seen that a resin support for the cotton swab is preferable compared to a wooden one.

[0139] <Reference Example 5> A plurality of substrates with saliva adhered were prepared in the same manner as in Reference Example 4, and amylase was quantified from each substrate in the same manner as in Reference Example 4. However, a cotton swab with a wooden support (manufactured by Nippon Cotton Swab Co., Ltd., examiner-attached cotton swab CTB-15) was used. Also, for the quantification of amylase, after sampling with a cotton swab, extraction was performed immediately, and the samples were divided into (i) a group in which the extract was stored at 4 °C for 4 days or 10 days and then quantified, and (ii) a group in which the extract was stored at 25 °C for 4 days or 10 days and then quantified. The results are shown in Table 8.

[0140]

Table 8

[0141] From Table 8, it can be seen that when the extract collected from the cotton swab is stored before the quantification of amylase, the detection sensitivity is maintained at both 4 °C and 25 °C if it is stored for 4 days. Also, since the detection sensitivity was maintained for a longer period at low temperature storage, it can be seen that low temperature storage is preferable when the process of storing the extract is included.

[0142] <Formulation Example> Tables 9 to 11 show formulation examples of the hygiene treatment agent that can be used for the hygiene treatment according to the present invention. Using these hygiene treatment agents, the hygiene treatment method of the present invention can be performed. However, the hygiene treatment agent is not limited to these. Table 9 is a treatment agent mainly used by impregnating sheet materials such as non-woven fabrics, and using this, a sheet-shaped hygiene treatment agent product can be obtained. The sheet-shaped hygiene treatment agent product is suitable for contacting and wiping the hygiene treatment agent at the treatment site. Table 10 is a treatment agent mainly used by spraying with a spray or the like, and using this, a spray-type hygiene treatment agent product can be obtained. The spray-type hygiene treatment agent product is suitable for efficiently contacting (spraying) the hygiene treatment agent at the treatment site). After contacting the hygiene treatment agent with the spray-type hygiene treatment agent product, if necessary, the treatment site may be wiped with a flexible material such as a non-woven fabric or a sponge. Table 11 is a treatment agent mainly used for washing and sterilization, and its product form can be various. For example, it can be used in the sheet-shaped hygiene treatment agent products or spray-type hygiene treatment agent products as described above.

[0143]

Table 9

[0144]

Table 10

[0145]

Table 11

[0146] Some of the components used in Tables 9 to 11 are as follows. · Amhil 20N: Manufactured by Kao Corporation, lauryldimethylamine oxide · Amhil 20AB: Manufactured by Kao Corporation, lauric acid amide propyl betaine · Neoperex GS: Manufactured by Kao Corporation, alkylbenzene sulfonic acid · Emal 20CS: Manufactured by Kao Corporation, sodium polyoxyethylene lauryl ether sulfate · Secondary alcohol EO 3 moles: A non-ionic surfactant obtained by adding an average of 3 moles of ethylene oxide to a secondary alcohol (with 12 to 14 carbon atoms), Softanol 30, manufactured by Nippon Shokubai Co., Ltd. · Alkyl glycoside: AG-124, manufactured by Kao Corporation, non-ionic surfactant · Leodol TW-L120: Manufactured by Kao Corporation, polyoxyethylene sorbitan monolaurate · Laurylamine EO adduct (2 moles): A non-ionic surfactant obtained by adding an average of 2 moles of ethylene oxide to laurylamine · Penetol GE-EH: Manufactured by Kao Corporation, 2-ethylhexyl glyceryl ether · Sanizole B-50: Manufactured by Kao Corporation, alkylbenzyldimethylammonium chloride · BDG: Butyl diglycol (diethylene glycol monobutyl ether), manufactured by Nippon Emulsion Co., Ltd., solvent · MFG: Propylene glycol monomethyl ether, manufactured by Nippon Emulsion Co., Ltd., solvent · Betaine polymer: A copolymer manufactured by the method of Example 1 of JP-A-2018-199770 · Carbopol ETD2020 POLYMER: Manufactured by Lubrizol Advanced Materials, carboxyvinyl polymer · Preservative: Kathon CG, manufactured by DuPont

Claims

1. A sanitation treatment method, which comprises detecting human salivary amylase in an environment by an immunochromatographic method, determining a location for sanitation treatment and / or sanitation treatment conditions based on the detection result, and performing sanitation treatment based on the determination.

2. The sanitation treatment method according to Claim 1, wherein the detection result includes quantification of human salivary amylase.

3. Once the location for sanitation treatment and / or sanitation treatment conditions are determined, sanitation treatment is performed without detecting human salivary amylase thereafter, or sanitation treatment is performed by detecting human salivary amylase periodically (excluding each time) or aperiodically. The sanitation treatment method according to Claim 1 or 2.

4. The sanitation treatment method according to Claim 1 or 2, wherein the environment is a living environment.

5. The sanitation treatment method according to Claim 4, wherein the environment includes an object to be detected for human salivary amylase, and human salivary amylase is detected on the surface of the object to be detected.

6. The sanitation treatment method according to Claim 1 or 2, wherein the sanitation treatment is a treatment using a sanitation treatment agent for at least any one selected from cleaning, sterilization, and virus killing.

7. The sanitation treatment method according to Claim 6, wherein the sanitation treatment agent contains one or more selected from (a) a surfactant, (b) an oxidative bleaching agent, and (c) a bactericide.

8. The sanitation treatment method according to Claim 6 or 7, wherein the sanitation treatment includes contacting the sanitation treatment agent with the treatment location and wiping the treatment location contacted with the sanitation treatment agent with a flexible liquid-absorbing material.

9. A sanitation treatment kit, which comprises means for detecting human salivary amylase in an environment by an immunochromatographic method and a drug used for treating a sanitation treatment location determined by the detection result of human salivary amylase obtained by the means.

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