Topical preparation for skin

Abstract

To provide a topical preparation for skin that is formulated with an active ingredient of natural origin, having superior biosafety, and exhibiting effects in prevention or improvement of spots, freckles, and dullness, and improvement of skin tone.SOLUTION: The present invention provides a topical preparation for skin in which a mixture of rosemary extract, sage extract, and lavender extract is used as an active ingredient.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to an external skin preparation containing, as an active ingredient, an extract of multiple plants belonging to the Lamiaceae family, which has excellent stability and efficacy, and which improves skin conditions and tone. [Background technology]

[0002] Skin aging or disorders is a phenomenon that occurs due to a complex interplay of internal factors, such as age-related inactivation of cell proliferation and differentiation, decreased hormone secretion, and quantitative decrease in extracellular matrix components, and external factors, such as cell and tissue damage or inflammation caused by reactive oxygen species induced by ultraviolet rays or air pollutants. Examples of skin aging or disorders include wrinkles, sagging, loss of firmness, decreased skin barrier function, pigmentation, dullness, etc., and anti-wrinkle agents, moisturizers, anti-blemish agents, etc. have been proposed depending on the respective skin conditions.

[0003] To date, various moisturizers (natural moisturizing factors [NHFs], glycerin, polyhydric alcohols, sodium lactate, etc.), antioxidants (vitamin E, astaxanthin, polyphenols, etc.), and agents for preventing or improving blemishes (placenta, ascorbic acid derivatives, ellagic acid, linoleic acid, etc.) have been proposed to prevent or improve skin aging, but there is a demand for ingredients derived from natural products that are less harsh on the skin. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 03-157334 [Patent Document 2] Japanese Patent Application Publication No. 06-024937 [Patent Document 3] Japanese Patent Publication No. 64-083022 [Patent Document 4] Japanese Patent Application Publication No. 09-002935 [Patent Document 5] Japanese Patent Application Publication No. 09-077636 [Patent Document 6] Japanese Patent Application Publication No. 09-136824 [Patent Document 7] Japanese Patent Application Publication No. 11-335233 [Patent Document 8] Japanese Patent Application Laid-Open No. 2015-028012 Summary of the Invention [Problem to be solved by the invention]

[0005] In view of the problems of the prior art, the present inventors have conducted extensive research to find new active ingredients derived from natural products that are safe for the skin. As a result, they have found that a mixture of an extract of rosemary (Lamiaceae, genus Salicornia), an extract of sage (Lamiaceae, genus Salicornia), and an extract of lavender (Lavender, genus Lavandula), has an excellent effect of improving skin conditions and skin tone.

[0006] It has been disclosed in Patent Documents 1 to 8, for example, that rosemary extract, sage extract, and lavender extract each have physiological skin activity on their own, but it has not been known that these extracts can be combined. [Means for solving the problem]

[0007] The present invention is an external skin preparation containing rosemary extract, sage extract, and lavender extract. [Effects of the Invention]

[0008] The present invention can provide an external skin preparation containing rosemary extract, sage extract, and lavender extract as active ingredients, which has the effect of improving skin conditions and skin tone. DETAILED DESCRIPTION OF THE INVENTION

[0009] The rosemary used in the present invention is Salvia rosmarinus, a member of the Lamiaceae family and the genus Salvia, and the plant species is not particularly limited. The part to be extracted is preferably the whole plant or the above-ground parts. The whole plant or the above-ground parts preferably include at least the flowers, leaves, and stems.

[0010] The sage used in the present invention is Salvia officinalis, a plant of the genus Salvia in the family Lamiaceae, but the plant species is not particularly limited. The whole plant or above-ground parts are preferred. The whole plant or above-ground parts preferably include at least the flowers, leaves, and stems.

[0011] The lavender used in the present invention is a plant of the genus Lavandula in the family Lamiaceae, and the species of the plant is not particularly limited. Examples include English lavender (Lavandula angustifolia), French lavender (Lavandula stoechas), and hybrids thereof. The part to be extracted is preferably the whole plant or above-ground parts. The whole plant or above-ground parts preferably include at least the flowers, leaves, and stems.

[0012] To prepare the extract, first, the part of each plant to be used is washed with water if necessary to remove foreign matter, and then, either as is or dried, shredded or crushed as necessary, and then brought into contact with an extraction solvent for extraction. Extraction can be carried out by contacting with the extraction solvent according to a conventional method such as immersion, but supercritical extraction can also be used instead of immersion.

[0013] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these may be used alone or in combination.

[0014] Among these extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoints of the efficacy of the resulting extract mixture, as well as skin irritation, and the wide applicability of these solvents to topical skin preparations (cosmetics, quasi-drugs, topical pharmaceuticals, etc.). Preferred examples of hydrophilic solvents include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol and 1,3-propanediol) alone, or a mixed solvent of water and lower alcohols (particularly ethanol), or a mixed solvent of water and polyhydric alcohols (particularly 1,3-butylene glycol and 1,3-propanediol). Among these, water alone, a mixed solvent of water and ethanol, a mixed solvent of water and 1,3-butylene glycol, or a mixed solvent of water and 1,3-propanediol are particularly preferred.

[0015] When a mixed solvent is used, the mixing ratio is preferably in the range of 5:1 to 1:5 by volume, for example, when the mixed solvent is a mixture of water and ethanol, water and 1,3-butylene glycol, or water and 1,3-propanediol.

[0016] The weight ratio of the part of each plant to be used to the extraction solvent is preferably 1:1 to 1:50.

[0017] When preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, the extraction solvent may be blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid to adjust the pH to the desired level.

[0018] The extraction conditions, such as extraction temperature and extraction time, vary depending on the type and pH of the solvent used. For example, when water alone or a mixed solution of water and ethanol, 1,3-butylene glycol, or water and 1,3-propanediol is used as the solvent, the extraction temperature is preferably in the range of 0°C to 80°C, more preferably in the range of 20°C to 60°C, and the extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week), more preferably in the range of 1 to 120 hours (1 hour to 5 days).

[0019] If necessary, the extract may be subjected to a hydrolysis treatment prior to or in parallel with the extraction treatment of the present invention, which may improve the storage stability of the extract and allow the extract to be used more effectively as a cosmetic ingredient.

[0020] The extracts prepared as described above are mixed and generally adjusted to a pH of 3 to 8. The mixture may be used as an ingredient in a topical skin preparation as is, or may be used at a desired concentration by vacuum concentration, etc. The extract may also be dried by a conventional method such as spray drying.

[0021] Examples of topical skin preparations (cosmetics, quasi-drugs, topical pharmaceuticals) containing the mixture of plant extracts of the present invention include, but are not limited to, emulsions, creams, lotions, essences, packs, lipsticks, foundations, sheet masks, liquid foundations, makeup press powders, blushers, face powders, facial cleansers, body shampoos, hair shampoos, cleansing cosmetics such as soaps, hair growth agents, and even bath additives.

[0022] When blended in an external skin preparation for skin care, the blending amount of each plant extract of the present invention is generally 0.00001 to 5.0 wt %, preferably 0.0001 to 1.0 wt %, in terms of the solid content of each extract. When blended in an external skin preparation for hair, the blending amount is generally 0.00001 to 5.0 wt % (solid content weight %, the same applies hereinafter) in terms of the solid content of each extract, preferably 0.0001 to 3.0 wt %.

[0023] When the mixture of the present invention is incorporated into topical skin preparations (cosmetics, quasi-drugs, topical pharmaceuticals, etc.), ingredients used in topical skin preparations, such as oily ingredients, surfactants (synthetic or natural), moisturizers, thickeners, emulsifiers or emulsifier aids, preservatives / bactericides, powder ingredients, UV absorbers, antioxidants, colorants, fragrances, anti-wrinkle agents, and other physiologically active ingredients, can be appropriately incorporated as needed. Furthermore, as long as the efficacy and characteristics of the apricot extract of the present invention are not impaired, it is also permissible to incorporate the apricot extract of the present invention in combination with other physiologically active ingredients into the topical skin preparation.

[0024] Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin wax. Examples of suitable glycerides include hydrocarbons such as cocos and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; and synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0025] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0026] Examples of emulsifiers and / or emulsifying aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc.

[0027] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, estradiol, and various amino acids and their derivatives.

[0028] Examples of thickeners include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0029] Examples of anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or a derivative thereof.

[0030] Examples of antiseptics and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and alkyl bromides. Examples of suitable ethanol include triisoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, radish fermented liquid, salus marvigor, and plant-derived ethanol such as corn, or 1,3-butylene glycol.

[0031] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0032] Anti-acne agents include, for example, sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0033] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybean, adzuki bean, etc.), etc.

[0034] Examples of ultraviolet absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-rosemary cinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-melosmary benzophenone-5-sulfonate, 4-tert-butyl-4-melosmary benzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0035] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), and the like.

[0036] Examples of whitening agents include one or more selected from ellagic acid and its derivatives, resorcinol derivatives, 4-mellowsmary cisalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate).

[0037] Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diaceroserosmary benzoic acid, 2-acerosmary-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid. Examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. Examples of the active ingredient include one or more selected from kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-mellowsmary cissalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, alpha-hydroxy acids, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid derivatives, mulberry bark extract, chamomile extract, hydrolyzed rice bran extract, saxifrage extract, and white mustard extract or hydrolyzates thereof.

[0038] Examples of kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of ascorbic acid derivatives include ascorbic acid ester salts such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate; ascorbic acid sugar derivatives such as ascorbic acid 2-glucoside, L-ascorbic acid 5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, 6-acylated products of these ascorbic acid sugar derivatives (acyl group is hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, and other L-ascorbic acid tetrafatty acid esters, 3-O-ethyl Ascorbic acid, L-ascorbic acid-2-phosphate-6-O-sodium palmitate, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, L-ascorbic acid aminopropyl phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. Hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), ) and the like, examples of tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (e.g., tranexamic acid methylamide), and the like, examples of resorcinol derivatives include 4-n-butylresorcinol, 4-isoamylresorcinol, and the like, examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diaceroserosmary citrate, 2-acerosmary citrate-5-hydroxybenzoic acid,Examples of nicotinic acid derivatives include nicotinamide (niacinamide) and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0039] Anti-wrinkle agents include vitamin A or its derivatives, vitamin E or its derivatives (tocopherol acetate, etc.), vitamin B or its derivatives (nicotinamide, etc.), and vitamin C or its derivatives (ascorbic acid glucoside, 3-O-ethyl ascorbic acid, ascorbic acid phosphate magnesium salt, etc.). Anti-inflammatory agents include D-pantothenyl alcohol, tranexamic acid, nicotinamide, allantoin, pyridoxine or its salt, and vitamin E or its derivatives (DL-α-tocopherol, DL-α-tocopherol acetate, etc.).

[0040] Furthermore, it is also possible to use in combination components derived from natural products such as the following plants or microorganisms: collagen or hydrolysates thereof, yeast extracts or hydrolysates, lactic acid bacteria cultures, grasses, cruciferous plants, Theaceae plants, Rosaceae plants, Paeoniaceae plants, Rutaceae plants, Amaranthaceae plants, Zosteraea plants, Leguminosae plants, Asteraceae plants, Fabaceae plants, Malvaceae plants, Gentianaceae plants, Lamiaceae plants, Nelumbosacaceae plants, Cucurbitaceae plants, Araliaceae plants, Solanaceae plants, Bignoniaceae plants, Actinidiaceae plants, Mulberry plants, Iridaceae plants, Campanulaceae plants, Oleaceae plants, Actinidiaceae plants, Mulberry plants, Rhamnaceae plants, Orchidaceae plants, and Anacardiaceae plants. Examples of suitable extracts include extracts of one or more plants selected from the family Garcinia, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae, or hydrolysates or fermented products thereof; extracts of one or more seaweeds selected from the family Laminaria, Mirrataceae, and Ulvulaceae, or hydrolysates or fermented products thereof; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts of royal jelly, or hydrolysates or fermented products thereof.

[0041] As ingredients derived from grass plants, particularly preferred are rice leaf hydrolysate, rice extract hydrolysate, rice bran extract hydrolysate, germinated brown rice hydrolysate, rice fermentation liquid, sake lees extract derived from sake, bamboo shoot skin extract from Madake or Moso bamboo, and fermented Job's tears seed. Furthermore, as Brassicaceae plants, particularly preferred are extracts of seeds of Hakugai, Ogai, or Kokugai, or hydrolysates or fermented products thereof. Furthermore, as ingredients derived from Theaceae plants, particularly preferred are green tea (Yabukita, Samidori, Asahi, Goko, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuuki, etc.) and black tea (Darjeeling, Assam, Ceylon, Earl Grey, Honey Black Tea, etc.). As ingredients derived from Rosaceae plants, preferred are Damask rose flower extract, peach flower, leaf, or immature fruit extract, strawberry flower extract, and cherry blossom flower or leaf extract. Furthermore, as components derived from Paeoniaceae plants, extracts of peony root or flower, and lavender flower are preferred. As components derived from Amaranthaceae plants, Salicornia extract is particularly preferred. As components derived from Zosteraea plants, extracts of Zostera marina or Zostera kohlrauss are particularly preferred. As components derived from Leguminosae plants, extracts of white soybeans or black soybeans or their hydrolysates, fermented soy milk, adzuki bean extract, red clover extract, and pueraria lobata root extract are particularly preferred. As components derived from Asteraceae plants, burdock root extract, sunflower sprout extract, Arctium gracilis extract, arnica extract, and chamomile flower extract are particularly preferred. As components derived from Malvaceae plants, fermented products of hibiscus, rose of sharon, or Hibiscus are preferred. As components derived from Gentianaceae plants, gentian extract is preferred. As components derived from Lamiaceae plants, perilla extract and barberry fruit extract are preferred. As a component derived from a plant of the Nelumbo family, particularly preferred is a lotus flower or lotus seed extract or a fermented lotus seed product. As a component derived from a plant of the Cucurbitaceae family, particularly preferred is a loofah extract. As a component derived from a plant of the Araliaceae family, a ginseng extract or fermented product is preferred. As a component derived from a plant of the Solanaceae family, an extract of eggplant (long eggplant, water eggplant, rice eggplant, Kamo eggplant, etc.) is included. As a component derived from a plant of the Bignoniaceae family, a Pau d'Arco bark extract is preferred. As a component derived from a plant of the Actinidiaceae family, an immature kiwi extract is preferred.As components derived from plants of the Moraceae family, mulberry bark extract, mulberry fruit extract, and fig fruit or bark extract are preferred. As components derived from plants of the Rhamnaceae family, jujube fruit extract is preferred. Furthermore, as components derived from plants of the Iridaceae family, saffron is preferred. As components derived from plants of the Campanulaceae family, an extract or hydrolysate of Codonopsis globulus root is preferred. As components derived from plants of the Anacardiaceae family, mango fruit extract is particularly preferred. As components derived from plants of the Garciniaceae family, mangosteen fruit extract is particularly preferred. Furthermore, as components derived from plants of the Valenciaceae family, cherimoya fruit extract is preferred. As components derived from plants of the Rutaceae family, extracts of mandarin oranges, bergamot fruit extracts, grapefruit or banpeiyu fruit (including immature fruits), extracts containing flavonoids and their glycosides contained in plants such as grapefruit or hassaku, or Japanese pepper seed extract are preferred. As components derived from plants of the Liliaceae family, extracts of daylily, daylily, Casablanca lily, Madonna lily, or Japanese lily are preferred. As components derived from plants of the Crassulaceae family, an extract or fermented product of Rhodiola rosea is particularly preferred. As components derived from plants of the Oleaceae family, jasmine flower extract is particularly preferred. As plants of the Cupressaceae family, juniper fruit extract is particularly preferred. As components derived from plants of the Myrtaceae family, guava leaf extract is particularly preferred. As plants of the Orchidaceae family, an extract of Bletilla sieboldii root (white orchid) is particularly preferred. As components derived from plants of the Convolvulaceae family, sweet potato extract or a fermented product thereof, or sweet potato shochu lees extract or a fermented product thereof is particularly preferred. As components derived from seaweeds of the Laminaceae family, kelp extract is particularly preferred, as components derived from seaweeds of the Mirinaceae family, Katamenkirinsai extract is preferred, and as components derived from seaweeds of the Ulva family, Ulva pertusa extract is particularly preferred. As the component derived from Funoriaceae seaweed, Funori extract is particularly preferred.

[0042] The present invention will now be described in more detail with reference to Production Examples, Formulation Examples, and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all % mean % by weight.

[0043] Preparation Example 1. Preparation of extract (1) Rosemary whole plants were dried, and 90 g of the dried product was added with 1125 g of purified water and 1125 g of absolute ethanol, followed by stirring and extraction at 40°C for 2 hours. This was filtered to obtain 1900 g of a brown, transparent 50 w / w% rosemary ethanol extract (solid content concentration 1.0%). The obtained 50 w / w% rosemary ethanol extract was concentrated to obtain 460 g of a concentrate. Purified water and 1,3-butylene glycol were added to the concentrate, and this was filtered to obtain 1190 g of a brown, transparent rosemary extract (solid content concentration 1.03%).

[0044] Preparation Example 1. Preparation of extract (2) Whole sage plants were dried, and 90 g of the dried product was mixed with 1125 g of purified water and 1125 g of absolute ethanol, followed by stirring and extraction at 40°C for 2 hours. This was filtered to obtain 1825 g of a brown, transparent 50 w / w% sage ethanol extract (solids concentration: 0.9%). The obtained 50 w / w% sage ethanol extract was concentrated to obtain 440 g of a concentrate. Purified water and 1,3-butylene glycol were added to the concentrate, and this was filtered to obtain 1250 g of a brown, transparent sage extract (solids concentration: 1.02%).

[0045] Preparation Example 3. Preparation of extract (3) The whole lavender plant was dried, and 400 g of purified water and 600 g of 1,3-butylene glycol were added to 100 g of the dried product, followed by stirring and extraction for 2 hours at 40°C. This was filtered, and purified water was added to obtain 1,300 g of a brown, transparent lavender extract (solid concentration 0.88%).

[0046] Example 1. Preparation of Mixture 1 300g of rosemary extract, 300g of sage extract, and 300g of lavender extract were mixed and filtered to obtain 900g of a brown mixture of rosemary extract, sage extract, and lavender extract (solid concentration 0.98%).

[0047] Although examples of preparation of each plant extract are shown in Production Examples 1 to 3, the present invention is not limited thereto. Similar to the solvents used in Production Examples 1 to 3, any polar solvent may be used as the extraction solvent, such as water alone, a mixed solvent of water and 1,3-butylene glycol, a mixed solvent of water and 1,3-propanediol, or a mixed solvent of water and ethanol.

[0048] Furthermore, the mixing ratio of the extracts in Production Examples 1 to 3 is not limited to the ratio shown in Example 1, but can be prepared so that the solid content ratio is rosemary extract:sage extract = 2:1 to 1:2, sage extract:lavender extract = 2:1 to 1:2, or rosemary extract:lavender extract = 2:1 to 1:2.

[0049] Test Example 1. Tyrosinase activity inhibition evaluation test (1) Normal human epidermal keratinocytes (NHEK) were placed in a 96-well plate at 8.0 × 10 3 Cells were seeded using HuMedia-KG2 (Kurabo Industries, Ltd.) at 100 cells / well and cultured for 24 hours. After replacing the culture medium with HuMedia-KB2 (Kurabo Industries, Ltd.), the extract of Production Example 1 was added at final concentrations of 0.25% and 0.125% in HuMedia-KB2. As a control, a 50% aqueous solution of 1,3-butylene glycol was similarly prepared and added. After culturing for 24 hours, the medium was replaced with HuMedia-KB2 containing no sample, and ultraviolet UV-B was irradiated at 50 mJ / cm to avoid sample carryover. 2 A control test group was set up without UV irradiation and used as a reference. 24 hours after UV irradiation, the culture supernatant was collected and frozen at -80°C. Normal human pigment cells (NHEM) were placed in a 96-well plate at 1.0 x 10 4Cells were seeded at 1000 cells / well using Derma Life M and cultured for 24 hours. After culture, the collected culture supernatant was added to the culture medium at 50% of the total volume, and culture was continued for 72 hours. After culture, the cells were washed once with PBS and lysed with PBS containing 1.0% Tween 20. L-DOPA was added to the cell lysate to a final concentration of 2.5 mM and incubated at 37°C. After 1 hour, the absorbance at 492 nm was measured and used as an index of intracellular tyrosinase activity. A separate plate under the same conditions was washed once with PBS, and a PBS test solution containing 0.3 mg / mL MTT was added and incubated at 37°C for 1 hour. The test solution was then removed using an aspirator, and 2-propanol was added to dissolve the cells. The absorbance at 570 nm was measured and used as an index of viable cell count. The tyrosinase activity per viable cell count was calculated by dividing the tyrosinase activity by the number of viable cells. The inhibition rate of tyrosinase activity was calculated by setting the value of the test group not irradiated with ultraviolet light as 100%.

[0050] The results of Test Example 1 are shown in Table 1. [Table 1] JPEG2025133008000001.jpg63132

[0051] As shown in Table 1, it was confirmed that tyrosinase activity in melanocytes was promoted when cultured epidermal keratinocytes were irradiated with ultraviolet light (UV-B) and the culture supernatant was added to melanocytes. In contrast, it was confirmed that tyrosinase activity was suppressed compared to the control when epidermal keratinocytes were cultured in the presence of the extract of Production Example 1 according to the present invention, even when irradiated with ultraviolet light (UV-B) and the culture supernatant was added to the melanocytes. This suggests that epidermal keratinocytes secrete a factor that promotes tyrosinase activity in melanocytes when irradiated with ultraviolet light (UV-B), and that the extract of Production Example 1 according to the present invention suppresses the promotion of tyrosinase activity by this factor.

[0052] Test Example 2. Tyrosinase activity inhibition evaluation test (2) Normal human fibroblasts (NB1RGB) were cultured in a 96-well plate at 1.0 × 10 4 The cells were seeded in Eagle's medium containing 0.5% NCS at 100 cells / well and cultured for 24 hours. The extract of Production Example 2 was prepared in Eagle's medium containing 0.5% NCS to a final concentration of 0.25% or 0.125%, and then added. As a control test, a 50% aqueous solution of 1,3-butylene glycol was prepared in the same manner and added. After 24 hours of culture, the medium was replaced with Eagle's medium containing 0.5% NCS but without the sample, and ultraviolet UV-A was applied at 4900 mJ / cm to avoid sample carryover. 2 A control test group was set up without UV irradiation and used as a reference. 24 hours after UV irradiation, the culture supernatant was collected and frozen at -80°C. Normal human pigment cells (NHEM) were placed in a 96-well plate at 1.0 x 10 4 Cells were seeded at 1000 cells / well using Derma Life M and cultured for 24 hours. After culture, the collected culture supernatant was added to the culture medium at 50% of the total volume, and culture was continued for 72 hours. After culture, the cells were washed once with PBS and lysed with PBS containing 1.0% Tween 20. L-DOPA was added to the cell lysate to a final concentration of 2.5 mM and incubated at 37°C. After 1 hour, the absorbance at 492 nm was measured and used as an index of intracellular tyrosinase activity. A separate plate under the same conditions was washed once with PBS, and a PBS test solution containing 0.3 mg / mL MTT was added and incubated at 37°C for 1 hour. The test solution was then removed using an aspirator, and 2-propanol was added to dissolve the cells. The absorbance at 570 nm was measured and used as an index of viable cell count. The tyrosinase activity per viable cell count was calculated by dividing the tyrosinase activity by the number of viable cells. The activity rate of tyrosinase was calculated by setting the value of the test area not irradiated with ultraviolet light as 100%.

[0053] The results of Test Example 2 are shown in Table 1. [Table 2] JPEG2025133008000002.jpg64133

[0054] As shown in Table 2, it was confirmed that tyrosinase activity in pigment cells was promoted when cultured fibroblasts were irradiated with ultraviolet light (UV-A) and the culture supernatant was added to pigment cells. In contrast, it was confirmed that tyrosinase activity was suppressed compared to the control when fibroblasts were cultured in the presence of the extract of Production Example 2 according to the present invention, even when fibroblasts were irradiated with ultraviolet light (UV-A) and the culture supernatant was added to pigment cells. This suggests that fibroblasts secrete a factor that promotes tyrosinase activity in pigment cells when irradiated with ultraviolet light (UV-A), and that the extract of Production Example 2 according to the present invention suppresses the promotion of tyrosinase activity by this factor.

[0055] Test Example 3. Tyrosinase activity inhibition evaluation test (3) Normal human microvascular endothelial cells (HMVEC) were plated in a 96-well plate at 1.0 × 10 4 The cells were seeded using HuMedia-MvG at 100 cells / well and cultured for 24 hours. The extract of Production Example 3 was added to HuMedia-MvG at final concentrations of 0.5%, 1.0%, and 2.0%. As a control, a 30% aqueous solution of 1,3-butylene glycol was similarly prepared and added. After 24 hours of culture, the cells were replaced with HuMedia-MvG containing no sample, and UV-A was irradiated at 4900 mJ / cm to prevent sample carryover. 2 A control test group was set up without UV irradiation and used as a reference. 24 hours after UV irradiation, the culture supernatant was collected and frozen at -80°C. Normal human pigment cells (NHEM) were placed in a 96-well plate at 1.0 x 10 4Cells were seeded at 1000 cells / well using Derma Life M and cultured for 24 hours. After culture, the collected culture supernatant was added to the culture medium at 50% of the total volume, and culture was continued for 72 hours. After culture, the cells were washed once with PBS and lysed with PBS containing 1.0% Tween 20. L-DOPA was added to the cell lysate to a final concentration of 2.5 mM and incubated at 37°C. After 1 hour, the absorbance at 492 nm was measured and used as an index of intracellular tyrosinase activity. A separate plate under the same conditions was washed once with PBS, and a PBS test solution containing 0.3 mg / mL MTT was added and incubated at 37°C for 1 hour. The test solution was then removed using an aspirator, and 2-propanol was added to dissolve the cells. The absorbance at 570 nm was measured and used as an index of viable cell count. The tyrosinase activity per viable cell count was calculated by dividing the tyrosinase activity by the number of viable cells. The inhibition rate of tyrosinase activity was calculated by setting the value of the test group not irradiated with ultraviolet light as 100%.

[0056] The results of Test Example 3 are shown in Table 3. [Table 3] JPEG2025133008000003.jpg74133

[0057] As shown in Table 3, when cultured vascular endothelial cells were irradiated with ultraviolet light (UV-A) and the culture supernatant was added to pigment cells, it was confirmed that tyrosinase activity in pigment cells was promoted. In contrast, when vascular endothelial cells were cultured in the presence of the extract of Production Example 3 according to the present invention, tyrosinase activity was suppressed compared to the control, even when vascular endothelial cells were irradiated with ultraviolet light (UV-A) and the culture supernatant was added to pigment cells. This suggests that vascular endothelial cells secrete a factor that promotes tyrosinase activity in pigment cells when irradiated with ultraviolet light (UV-A), and that the extract of Production Example 3 according to the present invention suppresses the promotion of tyrosinase activity by this factor.

[0058] From the evaluation test results of the extracts of Production Examples 1 to 3, it can be seen that Mixture 1, which is a combination of the extracts of Production Examples 1 to 3, can inhibit the factors secreted by three types of cells, epidermal cells, fibroblasts, and vascular endothelial cells, from increasing the tyrosinase activity of pigment cells due to ultraviolet rays, and is expected to be effective in preventing and improving age spots, freckles, and dullness, as well as improving skin tone.

[0059] Test Example 4: Test to evaluate the inhibition of tyrosinase activity in pigment cells by the culture supernatant of epidermal keratinocytes Normal human epidermal keratinocytes (NHEK) were plated in a 24-well plate at 1.0 × 10 5 Cells were seeded using HuMedia-KG2 (Kurabo Industries, Ltd.) at 1.5 cells / well and cultured for 24 hours. After replacing the culture medium with HuMedia-KB2 (Kurabo Industries, Ltd.), the sample solution described below was adjusted to a predetermined concentration using HuMedia-KB2 and added to the culture medium (test group). In addition, as a control group, a 1,3-butylene glycol aqueous solution was added to the culture medium at the same concentration as that in the test group. After continuing culture for 24 hours in the test and control groups, the medium was replaced with HuMedia-KB2 containing no sample solution or control solution, and ultraviolet UV-B was applied at 50 mJ / cm to avoid carryover of the sample solution. 2 A control group was set up without UV irradiation and used as a reference. 24 hours after UV irradiation, the culture supernatant was collected and frozen at -80°C. Normal human pigment cells (NHEM) were placed in a 96-well plate at 1.0 x 10 4 Cells were seeded at 100 cells / well using Derma Life M and cultured for 24 hours. After culture, the culture supernatant collected above was added to the culture medium at 50% of the total volume, and culture was continued for 72 hours. After culture, the cells were washed once with PBS, and PBS containing 1.0% Tween 20 was added to lyse the cells. L-DOPA was added to the cell lysate to a final concentration of 2.5 mM, and the cells were incubated at 37°C. The absorbance at 492 nm was measured after 1 hour. This was used as an index of intracellular tyrosinase activity. The inhibition rate of tyrosinase activity was calculated by setting the non-UV-irradiated test group at 100%.

[0060] The sample solutions used in Test Example 4 are shown in Table 4.5. The concentrations of the extracts of Production Examples 1 to 3 alone and the concentration of Mixture 1 (sample solution 4 in Table 1) shown in Example 1 indicate the final concentrations as solutions when added to the medium. The concentration of niacinamide (NA) indicates the concentration adjusted in advance.

[0061] [Table 4] JPEG2025133008000004.jpg36147

[0062] [Table 5] JPEG2025133008000005.jpg48129

[0063] The results of Test Example 4 are shown in Table 6. [Table 6] JPEG2025133008000006.jpg84121

[0064] As shown in Table 6, compared to the sample solutions (sample solutions 1 to 3) containing the extracts of Production Examples 1, 2, and 3 alone, it was confirmed that sample solution 4 (mixture 1) had a significant tyrosinase activity inhibitory effect. It was also confirmed that the mixed solution of the extracts of Production Examples 1 to 3 synergistically inhibited tyrosinase activity when used in combination with niacinamide. Thus, by incorporating the active ingredient of the present invention into a topical skin preparation, significant effects can be expected in preventing and improving age spots, freckles, and dullness, as well as improving skin tone.

[0065] In Test Example 4, sample solution 4 was used, in which the mixture ratio of the extract of Production Example 1, the extract of Production Example 2, and the extract of Production Example 3 was 1:1:1. However, similar excellent effects can be expected by adjusting the solid content ratio to extract of Production Example 1:extract of Production Example 2=2:1 to 1:2, extract of Production Example 2:extract of Production Example 3=2:1 to 1:2, or extract of Production Example 1:extract of Production Example 3=2:1 to 1:2.

[0066] In addition, a test was conducted using tranexamic acid instead of niacinamide in Test Example 4 above. Tranexamic acid was prepared so that the final concentration when added to the medium was 1.0%. Here, the tyrosinase activity inhibitory effects were compared between a sample solution containing only tranexamic acid (sample solution 10) and a sample solution containing tranexamic acid and Mixture 1 (adjusted to a final concentration of 0.3% as a solution) (sample solution 11). The results are shown in Table 7.

[0067] [Table 7] JPEG2025133008000007.jpg36119

[0068] As shown in Table 7, a significantly superior tyrosinase activity inhibitory effect was observed when tranexamic acid was combined with a mixed solution of the extracts of Production Examples 1 to 3. Furthermore, similar effects can be expected when used in combination with ascorbic acid derivatives, dipotassium glycyrrhizinate, arbutin, or kojic acid in addition to tranexamic acid or niacinamide, and furthermore, the effects of preventing and improving noticeable spots, freckles, and dullness, as well as improving skin tone, are suggested.

[0069] Prescription example 1. Lotion [Ingredients] Part mixture 1 0.9 Squalane 0.2 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Isopropylmethylphenol 0.1 1,3-butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Purified water (enough to make the total volume 100 parts)

[0070] Prescription example 2: Lotion [Ingredients] Part mixture 1 1.5 Glyceryl Caprylate 3.0 Polyglyceryl-10 Laurate 3.0 Cetyl alcohol 2.0 Behenyl Alcohol 2.0 Methylparaben 0.1 Niacinamide 5.0 Glycyrrhizic acid 0.5 Resorcinol 0.1 Zinc Oxide 2.0 dl-camphor 0.5 Glycerin 2.0 1,3-butylene glycol 5.0 Potassium hydroxide 0.5 Purified water (amount to make 100 parts)

[0071] Prescription example 3: Lotion [Ingredients] Part mixture 1 1.5 Jojoba oil 1.0 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Methylparaben 0.1 Ascorbic Acid Glucoside 2.0 Niacinamide 5.0 ε-aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Pyridoxine hydrochloride 0.5 Glycerin 5.0 1,3-butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-Camphor 0.1 Purified water (enough to make the total volume 100 parts)

[0072] Prescription example 4: Emulsion [Ingredients] Part mixture 1 1.5 Squalane 5.0 Cyclopentanesiloxane 1.0 Hexalan 3.0 Hexyldecyl Isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 Laurate 5.0 Polyglyceryl-10 Isostearate 5.0 Ascorbyl dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Ascorbic acid phosphate magnesium salt 3.0 Arbutin 3.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella fuciformis polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Isopropylmethylphenol 0.1 Water-soluble collagen 1.0 Hydrolyzed Collagen 1.0 Acetyl hyaluronic acid 0.1 Purified water (enough to make the total volume 100 parts)

[0073] Prescription example 5. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of L-ascorbic acid-2-glucoside was used in place of 2.0 parts of ascorbic acid phosphate magnesium salt.

[0074] Prescription example 6. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of ascorbic acid phosphate magnesium salt and 1.0 part of tranexamic acid were used in place of potassium hydroxide.

[0075] Prescription example 7. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of ascorbic acid phosphate magnesium salt was replaced with 3.0 parts of 3-O-ethyl ascorbic acid.

[0076] Prescription example 8. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of ascorbic acid phosphate magnesium salt and 5.0 parts of niacinamide were used in place of potassium hydroxide.

[0077] Prescription example 9. Cream [Ingredients] Part Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 1.0 Behenyl Alcohol 1.0 Stearyl Alcohol 1.0 Candelilla Wax 1.0 Lactic acid bacteria fermented rice 2.0 Soybean-derived hydrogenated lecithin 0.5 mixture 1 1.5 Carboxyvinyl Polymer 1.0 Sodium alginate 1.0 Glycerin 4.0 Preservatives 5.2 pH adjuster (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0078] Prescription example 10. Cream [Ingredients] Part mixture 1 1.5 Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Hexyldecyl Isostearate 5.0 Dioctyldodecyl / Phytosteryl Lauroyl Glutamate / Behenyl) 5.0 Glyceryl Caprylate 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 3.0 γ-oryzanol 0.1 Behenyl Alcohol 2.0 Palmitic acid 2.5 D-Pantothenyl Alcohol 3.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium chloride 0.05 Urea 3.0 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Ammonium glycyrrhizinate 0.1 Niacinamide 5.0 Lactic acid bacteria fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysolecithin 0.5 Hydrolyzed Collagen 1.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-camphor 0.3 l-menthol 0.5 Purified water (enough to make the total volume 100 parts)

[0079] Example 11. Pack [Ingredients] Part mixture 1 1.0 Dipropylene Glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetyl alcohol 3.0 Behenyl Alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Water-soluble collagen 1.0 Xanthan gum 2.0 Polyglyceryl-6 Myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated lecithin 1.0 Hydroxylated lecithin 1.0 Purified water (enough to make the total volume 100 parts)

[0080] Prescription example 12. Hair shampoo [Ingredients] Part Mixture 1 3.0 Sodium Laureth Sulfate 10.0 Glyceryl Monostearate 1.0 Coconut oil fatty acid diethanolamide 2.0 Polyoxyethylene (40) hydrogenated castor oil 0.5 Benzalkonium chloride 1.0 Stearyl Alcohol 2.0 Behenyl Alcohol 2.0 Dimethicone 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sodium salicylate 0.1 Tocopherol acetate 0.1 Pyrithione Zinc 0.3 Benzoic acid 0.2 Triclosan 0.2 Citric acid 0.1 Propylene Glycol 2.0 Purified water (enough to make the total volume 100 parts)

[0081] Prescription example 13. Hair conditioner [Ingredients] Part mixture 1 3.0 Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Benzalkonium chloride 1.0 Cetyl alcohol 3.0 Stearyl Alcohol 1.0 Allantoin 0.1 Isopropylmethylphenol 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sulfur 0.5 Alkylisoquinolinium bromide solution (75%) 0.06 Pyrithione Zinc 0.3 Methylparaben 0.1 Triclosan 0.2 Resorcinol 0.1 Purified water (enough to make the total volume 100 parts)

[0082] Formulation example 14. Cleansing cosmetics [Ingredients] Part Extract of Preparation Example 1 3.0 Potassium cocoyl glycinate 5.0 Glycerin 10.0 Glyceryl Caprylate 1.0 Sodium lauroyl aspartate 10.0 Water-soluble collagen 5.0 Cetyl alcohol 3.0 Myristyl Alcohol 3.0 Isopropyl methyl alcohol 0.1 Allantoin 0.1 Sulfur 0.5 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Monoammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.05 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.2 Tocopherol acetate 0.2 Triclosan 0.1 Trichlorocarbanide 0.5 Trichlorohydroxydiphenyl ether 0.2 Concentrated Benzalkonium Chloride Solution 50 0.2 Benzalkonium chloride 0.1 Purified water (enough to make the total volume 100 parts)

[0083] Prescription example 15. Sheet mask A sheet mask is obtained by impregnating a nonwoven fabric with the following ingredients. [Ingredients] Part mixture 1 1.5 Glycerin 3.0 1,3-butylene glycol 2.0 Niacinamide 5.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 1.0 Water-soluble collagen 1.0 Sodium hyaluronate 1.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0084] Prescription example 16. Beauty serum [Ingredients] Part mixture 1 1.5 Sodium hyaluronate 1.0 Water-soluble collagen 1.0 Tranexamic acid 0.1 Ethanol 2.0 Glycerin 5.0 1,3-butylene glycol 5.0 Methylparaben 0.1 Citric acid 0.3 Sodium citrate 0.6 Eucheuma muscaria extract 5.0 Purified water (enough to make the total volume 100 parts)

[0085] Prescription example 17. Cream [Ingredients] Part Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 1.0 Shea Butter 2.0 Behenyl Alcohol 1.0 Stearyl alcohol 1.5 Candelilla Wax 0.5 Niacinamide 5.0 mixture 1 1.5 Lactic acid bacteria fermented rice 3.0 Hydrogenated Lecithin 2.0 Eucheuma muscaria extract 2.0 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Glycerin 4.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

Claims

[Claim 1] An external skin preparation containing rosemary extract, sage extract, and lavender extract.

Images