Sterilization evaluation system

Abstract

To provide a sterilization evaluation system capable of performing quick sterilization evaluation to fine particles containing bacteria or viruses.SOLUTION: A sterilization evaluation system 1 has a first route 2 and a fine particle sampling device 31, where the first route 2 includes a first route wall 3, and a first space 4 surrounded by the first route wall 3, the first space 4 includes a supply space 5, a spraying space 8, an active space 9, a first inlet 10 and a first outlet 11, the supply space 5 is connected to a supply port 13 of a feeder 12 which feeds sterilization active substance, the spraying space 8 is connected to a spraying port 15 of a sprayer 14 which sprays fine particles containing bacteria or viruses, the active space 9 is a space where mixed air in which the sterilization active substance and fine particles are mixed passes, air flown from the first inlet 10 passes through the supply space 5 and the spraying space 8 to be mixed air, and the mixed air passes through the active space 9, the first outlet 11, a device inlet 31a, a device interior 31b and a device outlet 31c in this order.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present disclosure relates to a sterilization evaluation system. [Background technology]

[0002] Conventionally, as a method for evaluating sterilization of airborne particles containing bacteria or viruses, a sterilization evaluation system has been known in which the particles are sprayed into a closed evaluation chamber, brought into contact with a substance that has the effect of removing bacteria or viruses for a desired period of time, and then the particles are collected and detected.

[0003] For example, Patent Document 1 or Patent Document 2 discloses an environmental evaluation device in which a large number of air supply holes are provided on the entire surface of an evaluation chamber except for the floor, and air flows into the evaluation chamber through the air supply holes. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Laid-Open No. 2008-22764 [Patent Document 2] Japanese Patent Application Laid-Open No. 2008-22765 Summary of the Invention [Problem to be solved by the invention]

[0005] In such conventional sterilization evaluation systems, a particle sampling device for collecting particles is independently installed in a closed-space evaluation chamber into which particles containing bacteria or viruses are sprayed. Therefore, the amount of sterilizing substance and sprayed particles required is equal to the volume of the evaluation chamber. This has the problem that it takes a long time for the sterilizing substance and sprayed particles to uniformly diffuse throughout the evaluation chamber, and also takes a long time for the sprayed particles to reach an evaluable amount after contact with the sterilizing substance.

[0006] Therefore, the present disclosure was conceived in response to the above-mentioned conventional problems, and aims to provide a sterilization evaluation system that is capable of quickly evaluating the sterilization of fine particles (airborne fine particles) containing bacteria or viruses. [Means for solving the problem]

[0007] To achieve this object, a sterilization evaluation system according to one embodiment of the present disclosure is a sterilization evaluation system having a first path and a particle sampling device, wherein the first path includes a first path wall and a first space surrounded by the first path wall, and the first space includes a supply space, a spray space, an action space, a first inlet, and a first outlet, the supply space is connected to a supply port of a supplier that supplies a substance having the effect of removing bacteria or viruses, the spray space is connected to a spray port of a sprayer that sprays fine particles containing bacteria or viruses, the action space is a space through which mixed air containing the substance and fine particles passes, and the particle sampling device includes a device inlet, a device interior, and a device outlet, and samples fine particles, and by operation of a blower connected to the first space, air flowing in from the first inlet passes through the supply space and the spray space to become mixed air, and the mixed air passes through the action space, first outlet, device inlet, device interior, and device outlet in this order. [Effects of the Invention]

[0008] According to the present disclosure, it is possible to quickly evaluate the sterilization of microparticles containing bacteria or viruses. [Brief explanation of the drawings]

[0009] [Figure 1] 1 is a cross-sectional view showing the configuration of a sterilization evaluation system according to Example 1. [Figure 2] Flow diagram showing the series of operations of the sterilization evaluation system DETAILED DESCRIPTION OF THE INVENTION

[0010] Hereinafter, embodiments of the present disclosure will be described with reference to the drawings.

[0011] Example 1 As shown in FIG. 1, the sterilization evaluation system 1 includes a first path 2, a second path 21, and a particulate sampling device 31.

[0012] Disinfection refers to the removal of germs and / or viruses, including bacteria and / or fungi.

[0013] The first path 2 has a first path wall 3 and a first space 4 .

[0014] The first space 4 is a space surrounded by the first path wall 3. The first space 4 has, in order from upstream, a supply space 5, a diffusion space 6, a flow straightening space 7, a spray space 8, and an action space 9.

[0015] The supply space 5 is connected to a supply port 13 of a supply device 12 that supplies a substance having the effect of removing bacteria or viruses. The supply space 5 is connected to a humidity control port 19 of a humidity controller 18 that introduces humidified or dehumidified air (hereinafter referred to as dehumidified / dehumidified air).

[0016] Substances that have the ability to remove bacteria or viruses (hereinafter referred to as removal substances) include, for example, hypochlorous acid gas, ozone gas, positive and negative ions, radicals, and the like.

[0017] The feed space 5 has a first inlet 10 at its upstream end. The first inlet 10 is connected to an inlet filter 35.

[0018] Inlet filter 35 has an intake 33 at its upstream end.

[0019] The diffusion space 6 has a structure in which the opening area increases in the downstream direction. The diffusion space 6 is located between the supply space 5 and the spray space 8.

[0020] The rectification space 7 has a rectification section 20. The rectification space 7 is located between the diffusion space 6 and the spray nozzle 15. The rectification space 7 is also connected to the sensor inlet 17 of the gas sensor 16. The rectification section 20 is used to regulate the flow of air. In this embodiment, a grid or a metal grid is used for the rectification section 20.

[0021] The spray space 8 is connected to a spray nozzle 15 of a sprayer 14 that sprays fine particles B containing bacteria or viruses. The fine particles B include fungi, bacteria, viruses, aerosols, and the like.

[0022] The working space 9 is connected to the downstream end of the spraying space 8 and is a space through which mixed air containing the removal agent and the fine particles B passes. The working space 9 has a structure in which its length can be changed in the upstream and downstream directions of the air flow A, and has a first outlet 11 at its downstream end.

[0023] Here, a variable duct whose length can be changed may be used as the first path wall 3 surrounding the working space 9. This variable duct may be a flexible duct, an expandable duct, an extension duct, or the like.

[0024] The second path 21 has a second path wall 22 and a second space 23 .

[0025] The second space 23 has a second inlet 24 and a second outlet 25. The second space 23 is connected to a counter inlet 27 of a particle counter 26 that measures the number of fine particles. The second space 23 is connected to a temperature sensor 28 and / or a humidity sensor 29.

[0026] The particulate sampling device 31 includes a device inlet 31a, a device interior 31b, and a device outlet 31c. The device inlet 31a is connected to the first outlet 11. The device outlet 31c is connected to the second inlet 24.

[0027] The second outlet 25 is connected to an outlet filter 36. The outlet filter 36 has a discharge port 34 at its downstream end.

[0028] The blower 32 is connected to the exhaust port 34. The blower 32 is in communication with the first inlet 10, the first space 4, the first outlet 11, the device inlet 31a, the device interior 31b, the device outlet 31c, the second inlet 24, the second space 23, and the second outlet 25.

[0029] Next, the operation of the sterilization evaluation system 1 will be described with reference to Figures 1 and 2. As shown in Figure 2, the evaluator can evaluate one sample by performing operation steps S1 to S12 in order. If there are multiple samples to be evaluated, the evaluator repeats the series of operations from S1 to S12 the same number of times as the number of samples. In other words, if the operations from S1 to S12 are considered to be one series of operations, the evaluator needs to perform the series of operations the same number of times as the number of samples.

[0030] First, the evaluator operates the blower 32 (step S1: blowing step). The air taken in from the intake 33 by the blower 32 contains various particles originating from the outside air. This air passes through the inlet filter 35, whereby the particles contained in the air are removed. This cleans the air.

[0031] The purified air passes through the first inlet 10, supply space 5, diffusion space 6, rectification space 7, spray space 8, action space 9, first outlet 11, device inlet 31a, device interior 31b, device outlet 31c, second inlet 24, second space 23, second outlet 25, outlet filter 36, and exhaust port 34 in this order. This air is finally discharged from the exhaust port of blower 32. This air is illustrated by air flow A. In this example, the air flow rate of blower 32 was set to 100 L / min.

[0032] Next, the evaluator activates the supplier 12. The supplier 12 supplies the removal agent to the first space 4 (step S2: removal agent supply step). The removal agent supplied to the supply space 5 through the supply port 13 flows along the air flow A. At this time, the removal agent is diffused by passing through the diffusion space 6. The air containing the removal agent is then rectified by passing through the rectification space 7. This air flows uniformly through the spray space 8, the working space 9, the device interior 31b, and the second space 23, and is discharged from the outlet 34.

[0033] At this time, the gas sensor 16 measures the concentration of the removal substance supplied from the supplier 12. The evaluator adjusts the supply amount of the supplier 12 while observing the measurement value of the gas sensor 16 so that the desired removal substance concentration is obtained. In this example, hypochlorous acid gas generated by bubbling slightly acidic hypochlorous acid water was used as the removal substance. The evaluator then adjusted the supply amount of hypochlorous acid gas so that the measurement value of the gas sensor 16 was 100 ppb.

[0034] Next, the evaluator activates the humidity controller 18. The humidity controller 18 supplies dehumidified and humidified air to the first space 4 (step S3: humidity control step). The dehumidified air is diffused by passing through the diffusion space 6, similar to the removal substance described above. The dehumidified air is rectified by passing through the rectification space 7. The dehumidified air flows uniformly through the spray space 8, the working space 9, the device interior 31b, and the second space 23, and is discharged from the outlet 34. Therefore, the humidity in the spray space 8, the working space 9, the device interior 31b, and the second space 23 is essentially the same.

[0035] The evaluator adjusts the supply amount of the humidistat 18 while observing the measurement value of the humidity sensor 29 so that the humidity in the working space 9 becomes the humidity to be evaluated. In this example, the evaluator adjusted the supply amount of dehumidified / humidified air so that the humidity in the working space 9 becomes 60% RH.

[0036] Next, the evaluator activates the sprayer 14. The sprayer 14 sprays fine particles B containing bacteria and / or viruses from the spray nozzle 15 into the first space 4 (step S4: spraying step). The fine particles B sprayed into the spray space 8 flow along the air flow A, just like the removal agent and dehumidified air.

[0037] The particulate B and the removal agent mix in the working space 9. The air containing the particulate B and the removal agent is mixed air. A sterilizing effect is exerted on the particulate B in the mixed air while it passes through the working space 9. The particulate B that remains uncaptured by the particulate sampling device 31 passes through the second outlet 25 and is almost entirely captured by the outlet filter 36.

[0038] In this example, a Collison nebulizer or the like was used as the nebulizer 14. As an example, Staphylococcus epidermidis was 1×10 6 Phosphate buffered saline (PBS) suspended to a concentration of CFU / mL was sprayed using the sprayer 14. The sprayed fine droplets were designated as microparticles B.

[0039] Next, the evaluator activates the particle sampling device 31. The particle sampling device 31 samples the particles B that have flowed into the device inlet 31a (step S5: sampling step). In this example, an electrostatic collection type sampling device was used. The sampling time is arbitrary, and in this example, it was set to 5 minutes. The particle sampling device 31 captures particles from the air passing through the device interior 31b. The captured particles become part of the liquid sample.

[0040] After the sampling is completed, the evaluator promptly stops the sprayer 14. This stops the spraying of the fine particles B (step S6: spray stopping step).

[0041] Next, the evaluator stops the supplier 12. This stops the supply of the removal agent (Step S7: removal agent supply stopping step).

[0042] Next, the evaluator stops the humidity controller 18. This stops the supply of dehumidified and humidified air (step S8: humidity control stop step).

[0043] Next, the evaluator stops the fan 32. This stops the air flow A (step S9: air blowing temporary stop step).

[0044] Next, the evaluator collects the sample from the particle sampling device 31 (step S10: sample collection step). The collected sample contains particles B.

[0045] The assessor evaluates the number of bacteria or viruses contained in the collected sample. The method for detecting bacteria or viruses contained in the collected sample can be culture or staining method, etc. Any detection method may be used. In this example, the evaluator collected a liquid sample and performed a detection evaluation using the pour plate culture method.

[0046] The collected sample includes bacteria or viruses killed by the disinfectant. The number of bacteria or viruses evaluated by the evaluator is the number of live bacteria or viruses. In this case, the number of bacteria or viruses evaluated by the evaluator does not include the number of killed bacteria or viruses.

[0047] Next, the evaluator operates the blower 32 again. This causes the outlet filter 36 to capture the particles B remaining in the sterilization evaluation system 1 (step S11: particle removal step). At this time, the evaluator confirms that the concentration value of the particles B detected by the particle counter 26 is decreasing.

[0048] The evaluator confirms that the concentration value of the particles B is equal to or less than a predetermined safe concentration (step S12: safe concentration confirmation step). In this example, this safe concentration is set to 1 particle / cm 3 It was decided.

[0049] Next, the evaluator stops the blower 32. This stops the air flow A (step S13: blowing stop step).

[0050] The evaluation operation for one sample in the sterilization evaluation system 1 has been described above.

[0051] With this configuration, the air flowing in from the first inlet 10 passes through the supply space 5 and the spray space 8, becoming mixed air containing the removal agent and the fine particles B. This mixed air passes through the working space 9, the first outlet 11, the device inlet 31a, the device interior 31b, and the device outlet 31c, in that order. In other words, all of the fine particles sprayed into the spray space 8 mix with the removal agent that has already been uniformly dispersed within the working space 9, and after passing through the working space 9, quickly flow into the fine particle sampling device 31. This allows both sterilization and sampling to be performed in an extremely short time. As a result, it is possible to perform sterilization evaluation of fine particles containing bacteria and / or viruses in a short time, on the order of seconds.

[0052] Below, additional information will be provided regarding each example.

[0053] The inlet filter 35, diffusion space 6, rectification space 7, gas sensor 16, sensor inlet 17, humidity controller 18, humidity control port 19, rectification unit 20, particle counter 26, counter inlet 27, temperature sensor 28, humidity sensor 29, second path 21, outlet filter 36, etc. are not essential because the effects of the present disclosure can be obtained even without these.

[0054] For example, if already purified air is introduced through the first inlet 10, the inlet filter 35 is not required.

[0055] For example, when the sterilization evaluation system 1 and the evaluator are not in the same space, such as when the sterilization evaluation system 1 is remotely operated, the outlet filter 36 is not necessary.

[0056] The inlet filter 35 and / or the outlet filter 36 may be other means capable of capturing fine particles, provided that the blower 32 remains in communication with the first space 4.

[0057] Although the length of the working space 9 is variable, it may be fixed.

[0058] Although a metal grid is used for the flow straightening section 20, a perforated plate, a honeycomb grid, or the like may also be used.

[0059] Although an electrostatic collection type sampling device is used as the particulate sampling device 31, a filter type sampling device, a cyclone type sampling device, an impinger, or the like may also be used.

[0060] Although blower 32 is connected to the downstream end of outlet 34, it may also be connected to the upstream end of intake 33. In this case, the air flow generated by blower 32 passes through first inlet 10, supply space 5, spray space 8, action space 9, first outlet 11, device inlet 31a, device interior 31b, and device outlet 31c in this order.

[0061] It is not essential that the blower 32 be a stand-alone blower. For example, an exhaust system attached to the evaluation room in which the sterilization evaluation system 1 is installed may be used.

[0062] Although there are descriptions using the expression "all," such as "all particles," this is merely an example and may be interpreted as meaning "almost all" or "basically all."

[0063] Next, the effects of the present disclosure will be described.

[0064] The fine particles sprayed into the spray space 8 quickly flow into the working space 9 and mix with the removal agent, and are sampled by the fine particle sampling device 31 immediately after passing through the working space 9. Therefore, the sprayed fine particles hardly dry out, and sterilization evaluation of wet fine particles is possible. If sterilization evaluation of dry fine particles is desired, connecting a diffusion dryer between the sprayer 14 and the spray nozzle 15 also makes it possible to evaluate sterilization of dry fine particles.

[0065] Since the inlet filter 35 is connected to the upstream end of the first inlet 10, the air flowing into the first inlet 10 contains almost no fine particles. Therefore, it is possible to sample only the fine particles sprayed from the spray nozzle 15. As a result, highly accurate sterilization evaluation can be performed.

[0066] The sprayer 14 is connected to the spray space 8 through the spray nozzle 15, so that by changing the sprayer 14 as desired, it is possible to evaluate the sterilization effect on fine particles of different diameters.

[0067] The following items are independent of the scope of the claims. Although specific descriptions may be provided, they are merely examples and do not limit the scope of the claims.

[0068] (Item 1) A sterilization evaluation system 1 having a first path 2 and a particulate sampling device 31, The first path 2 includes a first path wall 3 and a first space 4 surrounded by the first path wall 3, The first space 4 includes a supply space 5, a spray space 8, an action space 9, a first inlet 10, and a first outlet 11; The supply space 5 is connected to a supply port 13 of a supply device 12 that supplies a substance having an effect of removing bacteria or viruses, The spray space 8 is connected to a spray nozzle 15 of a sprayer 14 that sprays fine particles containing bacteria or viruses. The action space 9 is a space through which mixed air containing the substance and the fine particles passes. The particle sampling device 31 includes a device inlet 31a, a device interior 31b, and a device outlet 31c, and is used to sample particles. By operating the blower 32 connected to the first space 4, the air flowing in from the first inlet 10 passes through the supply space 5 and the spray space 8 to become mixed air, and the mixed air passes through the action space 9, the first outlet 11, the device inlet 31a, the device interior 31b, and the device outlet 31c in this order.

[0069] According to this configuration, the fine particles sprayed from the spray nozzle 15 are mixed with the removal agent in the working space 9. After passing through the working space 9, these fine particles quickly flow into the fine particle sampling device 31 and can be sampled. This allows for rapid evaluation of sterilization of fine particles containing bacteria or viruses. In particular, sterilization evaluation can be performed in seconds.

[0070] (Item 2) The supply space 5 is located upstream of the spray space 8, The air flowing in from the first inlet 10 may pass through the supply space 5 and then through the spray space 8.

[0071] With this configuration, the removal agent supplied from the supply port 13 can be dispersed at a uniform concentration in the spray space 8 and the working space 9. The fine particles sprayed from the spray port 15 can be mixed with the removal agent dispersed at a uniform concentration in the working space 9. This allows for highly accurate evaluation of sterilization.

[0072] (Item 3) The first space 4 is connected to a sensor inlet 17 of a gas sensor 16 that detects the concentration of a substance. The sensor inlet 17 may be configured to be located downstream of the supply port 13 and upstream of the spray port 15.

[0073] With this configuration, the gas sensor 16 can measure the concentration of the substance in the first space 4. The amount of the removal substance supplied can be adjusted while monitoring the measured value, allowing for more accurate evaluation of sterilization.

[0074] (Item 4) The first space 4 is connected to a humidity control port 19 of a humidity controller 18 that introduces humidified or dehumidified air. The humidity control port 19 may be configured to be located upstream of the spray port 15.

[0075] According to this configuration, dehumidified and humidified air can be introduced from upstream of spray space 8, and the humidity in first space 4 can be controlled, allowing for more accurate evaluation of sterilization.

[0076] (Item 5) The first space 4 includes a diffusion space 6 whose opening area increases downstream, The diffusion space 6 may be located between the supply space 5 and the spray space 8 .

[0077] With this configuration, the flow of air flowing in from the first inlet 10 is stabilized by passing through the diffusion space 6. This diffusion space 6 makes it easier for the removal agent and / or dehumidified / humidified air supplied in the supply space 5 to diffuse uniformly. This allows for more accurate sterilization evaluation.

[0078] (Item 6) A rectifying section 20 for regulating the air flow is provided inside the first space 4, The flow regulating unit 20 may be configured to be located upstream of the spray nozzle 15.

[0079] According to this configuration, the flow of air flowing in from the first inlet 10 passes through the flow straightening section 20. This straightening section 20 makes it easier for the removal agent and / or dehumidified / humidified air supplied in the supply space 5 to be diffused uniformly, thereby enabling more accurate evaluation of sterilization.

[0080] (Item 7) The length of the working space 9 may be variable in the direction of flow of the mixed air.

[0081] According to this configuration, the time during which the mixed air passes through the interaction space 9 can be changed to arbitrarily change the time during which the removal agent acts on the microparticles containing bacteria or viruses, thereby enabling more accurate evaluation of sterilization.

[0082] (Item 8) having a second path 21; The second path 21 includes a second path wall 22 and a second space 23 surrounded by the second path wall 22, The second space 23 includes a second inlet 24 and a second outlet 25, The second inlet 24 may be configured to be connected to the device outlet 31c downstream of the device outlet.

[0083] (Item 9) The second space 23 may be configured to be connected to a counter inlet 27 of a particle counter 26 that counts the number of fine particles.

[0084] With this configuration, after sampling is completed, the number of particles remaining in the first space 4 and the second space 23 can be counted. Then, by operating the blower 32 until the count becomes sufficiently small (until the first space 4 and the second space 23 are clean), the risk of contamination can be reduced when the next sterilization evaluation is performed. This allows for more accurate sterilization evaluation.

[0085] (Item 10) a temperature sensor 28 and a humidity sensor 29; The temperature sensor 28 detects the temperature of the second space 23. The humidity sensor 29 may be configured to detect the humidity in the second space 23.

[0086] With this configuration, the temperature and / or humidity of the air flowing in from the first inlet 10 can be adjusted while monitoring the measured temperature and / or humidity values ​​in the second space 23, which is in communication with the first space 4. This allows for more accurate sterilization evaluation. As a method for adjusting the temperature and / or humidity, for example, the temperature and / or humidity may be adjusted by adjusting the temperature and / or humidity of a space external to the sterilization evaluation system 1. Furthermore, if the humidity control port 19 of the humidity controller 18 is provided in the first space 4, the humidity in the first space 4 may be adjusted by adjusting the amount of dehumidified / humidified air supplied by the humidity controller 18.

[0087] (Item 11) The second outlet 25 may have an outlet filter 36 .

[0088] According to this configuration, the fine particles discharged from the second outlet 25 can be collected by the outlet filter 36. This makes it possible to prevent the air discharged from the blower 32, the space outside the sterilization evaluation system 1, and the like from being contaminated by fine particles containing bacteria or viruses. As a result, the evaluator is hardly exposed to the fine particles, and sterilization evaluation can be performed safely. By using a high-precision filter as the filter 36, it is possible to capture all of the particulate matter discharged from the second outlet 25 in the outlet filter 36.

[0089] Although the sterilization evaluation system according to the present disclosure has been described above based on the examples, the present disclosure is not limited to the examples. As long as it does not deviate from the spirit of the present disclosure, various modifications conceivable by a person skilled in the art to each example and forms constructed by combining components in different examples are also included within the scope of the present disclosure. [Explanation of symbols]

[0090] 1. Disinfection evaluation system 2 Route 1 3 First Route Wall 4 1st space 5 Supply space 6 Diffusion Space 7 Rectification space 8 Spray space 9 Working space 10 First Entrance 11 Exit 1 12 Feeder 13 Supply port 14 Sprayer 15 Spray nozzle 16 Gas Sensor 17 Sensor inlet 18 Humidity controller 19 Humidity control outlet 20 Rectifier 21 Route 2 22 Second Route Wall 23 Second space 24 Second Entrance 25 2nd exit 26 Particle Counter 27 Counter entrance 28 Temperature Sensor 29 Humidity Sensor 31 Particulate sampling device 31a Equipment entrance 31b Inside the device 31c Equipment exit 32 Blower 33 Intake 34 Outlet 35 Inlet filter 36 Exit Filter A. Air flow B Fine particles

Claims

1. A sterilization evaluation system having a first path and a particulate sampling device, the first path includes a first path wall and a first space surrounded by the first path wall, the first space includes a supply space, a spray space, an action space, a first inlet, and a first outlet; The supply space is connected to a supply port of a supply device that supplies a substance having an effect of removing bacteria or viruses, The spray space is connected to a spray nozzle of a sprayer that sprays fine particles containing bacteria or viruses, the interaction space is a space through which mixed air containing the substance and the fine particles passes, the particulate sampling device includes an inlet, an interior, and an outlet, and samples the particulate; A sterilization evaluation system in which, by operation of a blower connected to the first space, air flowing in from the first inlet passes through the supply space and the spray space to become the mixed air, and the mixed air passes through the action space, the first outlet, the device inlet, the inside of the device, and the device outlet in this order.

2. The supply space is located upstream of the spray space, The sterilization evaluation system according to claim 1 , wherein the air flowing in from the first inlet passes through the supply space and then the spray space.

3. the first space is connected to a sensor inlet of a gas sensor that detects the concentration of the substance; The sterilization evaluation system according to claim 2 , wherein the sensor inlet is located downstream of the supply port and upstream of the spray port.

4. The first space is connected to a humidity control port of a humidity controller that introduces humidified or dehumidified air, The sterilization evaluation system according to claim 2 , wherein the humidity control port is located upstream of the spray port.

5. the first space includes a diffusion space whose opening area increases downstream, The sterilization evaluation system according to claim 2 , wherein the diffusion space is located between the supply space and the spray space.

6. a rectifying section for regulating the flow of air is provided inside the first space, The sterilization evaluation system according to claim 2 , wherein the flow straightening unit is located upstream of the spray nozzle.

7. The sterilization evaluation system according to claim 2 , wherein the length of the working space is variable in the direction in which the mixed air flows.

8. having a second pathway, the second path includes a second path wall and a second space surrounded by the second path wall, The second space includes a second inlet and a second outlet, The sterilization evaluation system according to claim 1 , wherein the second inlet is connected to the device outlet downstream of the device outlet.

9. The sterilization evaluation system according to claim 8 , wherein the second space is connected to a counter inlet of a particle counter that counts the number of the fine particles.

10. A temperature sensor and a humidity sensor are included. the temperature sensor detects the temperature of the second space; The sterilization evaluation system according to claim 8 , wherein the humidity sensor detects humidity in the second space.

11. The sterilization evaluation system according to claim 8 , further comprising an outlet filter at the second outlet.

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