Plant extract composition and use of the same

Abstract

To provide a plant extract composition capable of significantly promoting secretion of type I collagen (COL-I) and elastin that are anti-aging proteins.SOLUTION: A plant extract composition is provided, the plant extract composition containing cherry blossom extract and prickly pear extract, and the solid component weight ratio of the cherry blossom extract to the prickly pear extract being 75:25-10:90. Preferably, the plant extract composition does not contain any other plant extracts.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to a plant extract, a type I collagen production promoter, and an elastin production promoter. [Background technology]

[0002] As people's living standards improve and awareness of beauty and health grows, the demand for skin care and anti-aging measures is increasing day by day. Collagen and elastin are important proteins that maintain skin elasticity and firmness, and their production and maintenance are important for skin health. However, due to aging and the effects of environmental ultraviolet rays, the human body's own ability to synthesize collagen and elastin gradually weakens, resulting in aging phenomena such as wrinkles and sagging skin. Therefore, the search for methods or substances that can effectively promote collagen and elastin production has become a current research focus in the fields of beauty and biomedicine.

[0003] Cherry blossoms are known as a symbol of spring, and not only are they beautiful flowers, but their petals are rich in bioactive ingredients such as polyphenols and flavonoid compounds, which have a variety of biological activities, including antioxidant and anti-inflammatory properties. In recent years, cherry blossom extracts have been gaining attention for their application in the fields of beauty and skin care, and are expected to promote skin metabolism and improve skin quality.

[0004] Rosa roxburghii (also known as wild pear or mountain pear (Rosa roxburghii Tratt)) is a widely distributed wild fruit tree whose fruit contains abundant vitamin C, flavonoid compounds, polyphenols, and other substances, and has significant antioxidant and anti-inflammatory properties. In traditional medicine, Rosa roxburghii is used to treat various diseases, including skin disorders. Recent studies have shown that Rosa roxburghii extract can promote the proliferation and differentiation of skin cells, and has a positive effect on improving skin quality and promoting collagen synthesis.

[0005] Although cherry blossom extract and thorny pear extract have achieved some success in the application of beauty and skin care, previous research results still have room for improvement in the effect of promoting collagen and elastin production. To satisfy people's pursuit of beauty and health, products with better promoting effects have important practical significance and market value. Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide a plant extract composition that can significantly promote the production of collagen and elastin. [Means for solving the problem]

[0007] After extensive research, the inventors discovered that when cherry blossom extract and prickly pear extract are used in combination, a superior collagen and elastin production promoting effect is achieved compared to when either extract is used alone at the same dosage, leading to the completion of the present invention.

[0008] The present invention includes the following embodiments. [1] A plant extract composition comprising a cherry blossom extract and a prickly pear extract, wherein the weight ratio of the solid components of the cherry blossom extract to the prickly pear extract is 75:25 to 10:90. [2] The composition described in [1], which does not contain other plant extracts. [3] Use of the composition according to [1] or [2] in food. [4] Use of the composition according to [1] or [2] in cosmetics. [5] Use of a composition containing cherry blossom extract and prickly pear extract in the manufacture of a type I collagen production promoter. [6] Use of a composition containing cherry blossom extract and prickly pear extract in the manufacture of an elastin production promoter.

[0009] The plant extract composition of the present application may be a solid composition or a liquid composition prepared to a certain concentration. [Effects of the Invention]

[0010] The composition of the present invention can significantly promote the secretion of type I collagen production promoter (COL-I) and elastin, which are anti-aging proteins. DETAILED DESCRIPTION OF THE INVENTION

[0011] The plant extract composition of the present invention contains a cherry blossom extract and a Prickly Pear extract, and the weight ratio of the solid components of the cherry blossom extract to the Prickly Pear extract is 75:25 to 10:90.

[0012] In the present invention, the composition having the above-mentioned configuration can significantly improve the secretion amounts of type I collagen and elastin, and in particular, can achieve a promotion effect that exceeds that achieved when cherry blossom extract or prickly pear extract is used alone.

[0013] In the present invention, the cherry blossom extract, which is the active ingredient, may be a water extract or an ethanol extract, but is preferably a water extract. The cherry blossom extract used in the present invention may be a commercially available product or may be obtained by a known extraction method, such as an extract produced by extracting dried cherry blossoms or fresh Sekiyama cherry blossoms with water or a mixture of water and ethanol.

[0014] Specifically, the following method can be used: Dried cherry blossom raw material is prepared, and defective products, such as spoiled raw material, are removed. The mixture is then placed in an extraction tank, purified water is added, and the mass ratio of water to raw material is controlled to 50:1 to 10:1. The mixture is heated to 40 to 80°C and extracted for 0.5 to 10 hours. The residue is then removed by filtration, and the resulting extract is filtered through a 50 to 500 nm ceramic membrane, concentrated using an ultrafiltration membrane, and then freeze-dried to obtain a powder. Alternatively, powder can be obtained by adding 20 to 50% by mass of maltodextrin, starch, etc. after concentration and spray-drying. Here, 20 to 50% by mass refers to the mass concentration of the auxiliary ingredients in the finished powder product after drying.

[0015] In the present invention, the active ingredient, Prickly Pear extract, may be a water extract or an ethanol extract, preferably a water extract. The Prickly Pear extract used in the present invention may be a commercially available product or one obtained by a known extraction method, for example, an extract produced by squeezing fresh Prickly Pear juice, filtering to remove particulate matter and polymeric substances, and then concentrating the juice.

[0016] Specifically, the following method can be used: Fresh tangerine pears are prepared, washed, and then squeezed to obtain tangerine pear juice. The juice is filtered through a 100-300 mesh filter cloth, and the filtrate is filtered through a 50-500 nm ceramic membrane cartridge to remove particulate matter and polymeric substances. The resulting filtrate is vacuum-concentrated at low temperatures at 40-70°C to obtain an extract with a solid content of 20% to 40% by mass. A certain amount of auxiliary ingredients (e.g., 20-50% by mass of maltodextrin, starch, etc.) is added to the extract, which is then dried using a spray dryer or freeze dryer to obtain powder. Here, 20-50% by mass refers to the mass concentration of the auxiliary ingredients in the finished powder product after drying.

[0017] The freeze-drying process is roughly as follows: first, the material is pre-frozen to -40°C under vacuum and held at that temperature for several hours, typically 4 to 6 hours; then, the material moves to the sublimation drying stage, where the temperature is gradually raised from -40°C to -20 to -15°C and held at that temperature for 5 to 12 hours; then, the material is further raised from -20 to -15°C to -10°C and held at that temperature for 10 to 24 hours; and finally, the material moves to the analytical drying stage, where the temperature is gradually raised from -10°C to 30 to 40°C and held at that temperature for approximately 10 hours, yielding the freeze-dried product. Then, the mixture was crushed and sieved to obtain a finer powder.

[0018] After extensive research, the inventors have found that cherry blossom extract and Chinese pear extract have a synergistic effect in promoting collagen and elastin secretion, and that a particularly significant synergistic effect is achieved when cherry blossom extract and Chinese pear extract are used together in a solid component weight ratio of 90:10 to 10:90.

[0019] Furthermore, when the weight ratio of the solid components of the cherry blossom extract and the prickly pear extract was set to 75:25 to 10:90, a more pronounced synergistic effect was confirmed. [Example]

[0020] The technical concept of the present invention will be further explained below with reference to examples. However, the following examples are merely illustrative examples for those skilled in the art to clearly understand the concept of the present invention, and are not intended to specifically limit the present invention.

[0021] Raw material extract manufacturing (1) Production of cherry blossom extract Dried Sekiyama cherry blossom raw material was prepared, and defective products, such as spoiled raw materials, were removed. The mixture was then placed in an extraction tank, purified water was added, and the mass ratio of water to raw materials was controlled to 20:1. The mixture was heated to 50°C and extracted for 1 hour. The residue was then removed by filtration, and the extract was filtered through a 200nm ceramic membrane and concentrated using an ultrafiltration membrane. 20% maltodextrin was then added and spray-dried to obtain a powder. (2) Manufacturing of Prickly Pear Extract Fresh tangerine pears were prepared, washed, and squeezed to obtain tangerine pear juice. The juice was filtered through a 200-mesh filter cloth, and the filtrate was filtered through a 200-nm ceramic membrane cartridge to remove particulate matter and polymeric substances. The resulting filtrate was vacuum-concentrated at 60°C to obtain an extract with a solid content of 40% by mass. 20% by mass of maltodextrin was added to the extract, which was then dried in a spray dryer to obtain tangerine pear extract powder.

[0022] Preparation of the composition The Guanshan cherry blossom extract and the prickly pear extract powder were weighed out and mixed in a certain weight ratio to form a composition.

[0023] Example 1 Guanshan cherry blossom extract-Chinese pear extract composition (cherry blossom-Chinese pear composition): Guanshan cherry blossom extract and Chinese pear extract powder were weighed out and mixed in a weight ratio of 90:10, and a sample solution of the appropriate concentration was prepared using sterile PBS buffer. The sample was passed through a 0.22 μm filtration membrane and then prepared for measurement.

[0024] Example 2 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was set to 75:25.

[0025] Example 3 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was 65:35.

[0026] Example 4 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was 60:40.

[0027] Example 5 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract and tangerine pear extract was 50:50.

[0028] Example 6 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was 40:60.

[0029] Example 7 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was 35:65.

[0030] Example 8 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was 30:70.

[0031] Example 9 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was set to 26:74.

[0032] Example 10 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was set to 25:75.

[0033] Example 11 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was 20:80.

[0034] Example 12 A composition solution was obtained in the same manner as in Example 1, except that the weight ratio of cherry blossom extract to tangerine pear extract was 10:90.

[0035] Comparative Example 1 A composition solution was obtained in the same manner as in Example 1, using the cherry blossom extract prepared above, but without adding the tangerine extract.

[0036] Comparative Example 2 A composition solution was obtained in the same manner as in Example 1, using the above-prepared tangerine extract and without adding cherry blossom extract.

[0037] <Evaluation of the effect of the composition on promoting type I collagen and elastin secretion in a UVA-damaged fibroblast model>

[0038] 1. Test equipment Carbon dioxide incubator (Thermo), microplate reader (Tecan), inverted microscope (CEWEI), water bath, vortex mixer, electronic balance, ultraviolet irradiation lamp (Philips), dosimeter (TENMARS).

[0039] 2. Materials and reagents Fetal bovine serum (FBS) (Gibco), high-glucose DMEM medium (Gibco), fibroblast HSF (Chinese Academy of Sciences Cell Bank), double antibiotics (penicillin + streptomycin, Gibco), complete medium (10% FBS + 90% high-glucose DMEM + 1% double antibiotics), COL-I detection kit (Elabscience), and elastin detection kit (Elabscience).

[0040] 3, cell culture 3.1. Cell thawing and passaging Fibroblasts were removed from liquid nitrogen and quickly thawed in a 37°C water bath. They were then transferred to 5 ml of preheated medium, centrifuged at 1000 rpm for 5 minutes, and the supernatant discarded. The cells were then transferred to preheated complete medium and gently pipetted to resuspend the cells. Fibroblasts that reached 80-90% confluence were washed twice with PBS, digested with 0.25% trypsin, and incubated at 37°C for 1 minute. The digestion was stopped with medium, centrifuged at 1000 rpm for 5 minutes, the supernatant discarded, transferred to preheated complete medium, gently pipetted to resuspend the cells, and then subcultured.

[0041] 3.2 Cell culture and seeding For cell culture, high-glucose DMEM medium (containing 10% FBS and 1% dual antibiotics) was used. Fibroblasts were harvested and counted when they reached 80-90% confluence. Based on the counting results, 2-8 × 10 cells were cultured in a 24-well cell culture plate. 4 The cells were seeded at a density of 1000 cells / ml, and 500 μl was dispensed into each well.

[0042] 3.3. Drug administration Based on the experimental design, the group administration was carried out. The administration volume in each well was 500 μl, and three multiple holes were set up in each group. The dose was 5 J / cm except for the control group. 2 The cells were irradiated with UVA and cultured in an incubator (37°C, 5% CO2, 95% RH) for 24 hours.

[0043] 3.4. Result detection The supernatant of the cell culture was collected and centrifuged at 12,000 rpm for 2 minutes, after which the supernatant was collected, and the concentrations of type I collagen (COL-I) and elastin in each sample were detected using a COL-I detection kit and an elastin detection kit, respectively. Blank control group: Samples that were not UVA irradiated or administered with any drug. UVA irradiation model control group: Samples that were irradiated with UVA but not administered with any drugs. Drug-administered sample group: Samples to which the drug was administered at a concentration of 0.5 mg / ml or 0.1 mg / ml and which were also irradiated with UVA. The UVA-irradiated model control group was exposed to UVA irradiation, and as a result, the secretion levels of type I collagen and elastin were significantly reduced compared to the control sample. Despite UVA irradiation, the drug-administered group was able to stimulate fibroblasts to secrete type I collagen and elastin, resulting in increased secretion compared to the UVA-irradiated model control group. Therefore, by calculating the rate of change in protein secretion between the drug-administered sample group and the UVA-irradiated model control group, it is possible to evaluate the difference in the protein secretion stimulating effect of the drug-administered composition. The effects of the compositions of each example and comparative example on the production of type I collagen and elastin by fibroblasts in the blank control group, UVA-irradiated model control group, and drug-administered sample group are shown in Tables 1 and 2 below.

[0044] [Table 1] [Table 2]

[0045] Table 2: Effect of blank control group, UVA-irradiated model control group, and drug-administered sample group on elastin production

[0046] As is clear from the data of the above Examples and Comparative Examples, the control group, after exposure to UVA irradiation, showed reduced production of type I collagen and elastin by fibroblasts compared to the blank group not exposed to UVA irradiation. On the other hand, the sample group, despite being exposed to UVA irradiation, produced more type I collagen and elastin after treatment with cherry blossom extract and pear extract compared to the blank group simply exposed to UVA irradiation.

[0047] In the sample group, a significant synergistic effect was observed when cherry blossom extract and prickly pear extract were used together. That is, when used together, the effect of promoting type I collagen and elastin secretion was significantly higher than when used alone at the same dosage. In particular, a more significant synergistic effect was observed when cherry blossom extract and prickly pear extract were used in a weight ratio of 75:25 to 10:90.

[0048] In measuring the secretion levels of type I collagen and elastin, the concentrations of the active ingredients (cherry blossom extract and / or prickly pear extract) in the fibroblast cultures were determined for in vitro experiments, not the concentration of the composition itself. The composition products of the present application may be solid compositions or liquid compositions prepared to a specific concentration as needed. Those skilled in the art can select an appropriate product form and active ingredient concentration based on the technical concepts of the present application and in combination with the needs of actual applications.

[0049] The composition of the present application can be used for producing a type I collagen production promoter, for producing an elastin production promoter, for food applications, and for cosmetics applications.

Claims

1. A plant extract composition comprising a cherry blossom extract and a prickly pear extract, wherein the weight ratio of the solid components of the cherry blossom extract to the prickly pear extract is 75:25 to 10:

90.

2. 10. The composition of claim 1, which does not contain other plant extracts.

3. Use of the composition according to claim 1 or 2 in food products.

4. Use of the composition according to claim 1 or 2 in cosmetics.

5. Use of a composition containing cherry blossom extract and tangerine pear extract in the manufacture of a type I collagen production promoter.

6. Use of a composition containing cherry blossom extract and thorn pear extract in the manufacture of an elastin production promoter.

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