Single dose cosmetic product and its manufacturing method

Abstract

The present invention relates to a single-dose cosmetic product and a method for producing the same. The single-dose cosmetic product of the present invention comprises a solid portion of the soft tissue of the aloe vera plant, a coating having waxy properties that completely encapsulates the solid portion, and at least one tear tape located between the coating and the solid portion. The cosmetic product of the present invention allows better handling and application of aloe vera, and also retains the properties and stability of 100% aloe vera product without the use of additives. The method for producing the cosmetic product can keep the properties of aloe vera soft tissue intact.

Description

Detailed Description of the Invention

[0001] [Field of the Invention] The present invention relates to a cosmetic product. In particular, the present invention relates to a single-dose cosmetic product comprising a solid portion of the soft tissue of the Aloe vera plant and a coating having waxy properties that completely encapsulates the solid portion.

[0002] The present invention also relates to a method for producing said cosmetic product.

[0003] [Background technology] Aloe vera belongs to the Malvaceae family. It is a succulent, soft plant with a high water content (approximately 99-99.5%). Its solid content is 0.5-1% and it is composed of various active ingredients such as fat-soluble and water-soluble minerals, vitamins, simple / complex polysaccharides, organic acids, enzymes, and phenolic compounds.

[0004] The fleshy leaves, arranged in a rosette, are composed of three layers. The outer layer is made up of the bark or rind, which accounts for 20-30% of the plant's total weight and is green or blue-green in color. The middle layer is called the parenchyma, also known as the fillet, pulp or gel, which is transparent, has a gel-like fibrous matrix and accounts for 50-60% of the plant's total weight. Between the rind and the parenchyma, which occupy the entire inner surface of the leaf, are the aloin canals, which are longitudinally oriented tubes in which the acybars, also called latex, circulate.

[0005] Aloe vera is well known for its therapeutic properties. Many beneficial effects have been reported, including immunomodulatory, wound and burn healing, hypoglycemic, anticancer, gastrointestinal protective, antigunfal and anti-inflammatory properties. These beneficial therapeutic properties of Aloe vera have been adopted for several commercial applications.

[0006] As a beauty ingredient, Aloe vera is widely used in many mainstream skin care and cosmetic products. The plant is extremely gentle and versatile with a low risk of causing allergies, hypersensitivity, or skin reactions. Aloe vera is commonly used in tanning products, as well as in face, hair, and body care products.

[0007] Cosmetics or compositions containing a high percentage of Aloe vera are always accompanied by other compounds or active ingredients, such as preservatives or antibacterial agents, to prevent the deterioration of the properties of Aloe vera, and other chemical products that help to stabilize and preserve the cosmetic or composition.

[0008] For example, WO2009112558A1 describes a composition used as a base for cosmetics, which contains 50% by weight to 99% by weight of aloe vera gel, an emulsifier containing an acryloyl lauric acid copolymer, a nonionic surfactant consisting of an ester of sorbitol polyethoxylate, and a naturally derived oil.

[0009] JP 2003-252719A relates to a cosmetic product mainly composed of aloe vera gel juice. The product contains aloe vera gel juice (75-83% by weight), 1,3 butylene glycol, glycerin, and sodium hyaluronate. The active ingredients moisturize the skin, prevent skin blemishes and freckles, and promote collagen regeneration and the activity of superoxide decomposition enzymes in the skin.

[0010] However, the use of some chemical compounds in these compositions may have adverse effects on consumer health.There is also a growing interest in 100% natural cosmetic products that do not contain these chemical compounds or other additives.

[0011] However, it is difficult to maintain the properties of the compounds contained in cosmetics without using such chemical compounds. In the case of Aloe vera, the full range of benefits of Aloe can be obtained most abundantly by extracting Aloe vera products directly from the plant, but the active ingredients in Aloe vera deteriorate over time, making it difficult to maintain all of the properties.

[0012] One of the methods used to prevent deterioration of its properties and extend the shelf life of pure Aloe vera is to freeze the Aloe vera gel, as described in EP 3067060 A1.

[0013] EP3067060A1 relates to a process that allows the use of pure Aloe vera gel, containing all the natural properties of the Aloe vera plant, incorporating a stabilization process achieved by freezing, which allows the topical application of cold creams, lotions, pomades or ointments, achieving all the typical uses of Aloe vera (but mainly for relieving burns of any kind). The gel is stored individually in containers of PET or similar plastic, designed to withstand temperatures as low as -19°C without suffering any damage. The resulting product is a natural Aloe vera cold gel, free of stabilizing agents.

[0014] The disadvantages of EP3067060A1 are as follows: the steps used in the method to obtain the product, such as crushing and filtering Aloe vera, change its natural structure and cause the loss of its functional molecules, such as polysaccharides, vitamins, minerals, etc. Furthermore, the process also causes the loss of endophytic microorganisms living in the plant tissue of Aloe vera.

[0015] In addition, aloe vera gel is subjected to a freezing treatment which stabilizes the gel, however, although freezing inactivates certain microorganisms, it is not fully effective unless combined with other treatments (heating, irradiation, acidification, etc.).

[0016] Moreover, storage in such plastic containers does not prevent the aloe vera gel from deteriorating due to temperature differences, exposure to light, and other storage factors that directly affect shelf life. This last aspect is also influenced by the fact that the product must be stored at low temperatures, necessitating special conditions and thus creating difficulties in transportation, storage, and handling.

[0017] Therefore, there is a need to find new cosmetic products based on Aloe vera that combine all the properties of pure Aloe vera and are stable for a long period of time, without the need for the incorporation of chemical compounds such as stabilizers or preservatives and / or the use of complex manufacturing processes.

[0018] [Summary of the Invention] In view of the problems of the prior art, the primary objective of the present invention is to provide a single-dose cosmetic product having a solid portion of the parenchyma of the aloe vera plant, a coating having waxy properties that completely encapsulates the solid portion, and at least one tear tape located between the coating and the solid portion.

[0019] The cosmetic product of the present invention solves the technical problem of preserving the properties and stability of a 100% aloe vera product without the use of additives.

[0020] The main technical difference with conventional cosmetics lies in the fact that this novel cosmetic product is like a 100% pure / natural plant that is applied directly to the skin, without any other ingredients that dilute its effectiveness, preservatives, colorants, artificial fragrances or any objectionable substances that degrade the cosmetic product.

[0021] Another unexpected technical difference is that the cosmetic product of the present invention allows the incorporation of various endophytic microorganisms naturally occurring in this plant, such as Pantoea agglomerans, Yersinia kristensenii, Erwinia billingiae, Rahnella Victoriana, Pantoea allii, Serratia liquefaciens, Yersinia ruckeri, Rahnella woolbedingensis, Leclercia adecarboxylata, Serratia grimesii, Rahnella inusitata, and the like. The key to the success of this treatment is the preservation of the survival of the endogenous microorganisms Pantoea inusitata and Pantoea ananatis. These endogenous microorganisms are natural probiotics present in various plant tissues (parenchyma) and have beneficial effects on the skin's immune system and the treatment of skin diseases.

[0022] Conventional cosmetic products, whether in the form of gels, creams or ointments, cannot contain these endophytic microorganisms because the manufacturing process involved necessarily destroys the tissue of the aloe plant on which these microorganisms live.

[0023] On the other hand, conventional cosmetics are commercially available in the form of gel, cream, or ointment, as described above. When applying such cosmetics, users may not be able to apply an appropriate amount or the texture of the product may be difficult to handle, resulting in insufficient application of the cosmetics by users.

[0024] The cosmetic product of the present invention is in the form of a natural piece of plant covered with a coating having waxy properties, allowing better handling of the aloe vera and preserving its properties.Furthermore, the cosmetic product of the present invention contains a portion of the aloe vera parenchyma in an amount suitable for a single administration.

[0025] Additionally, the coating having waxy properties facilitates the manipulation of the parenchyma pieces of the aloe vera plant, which can be difficult for users to use due to the slippery product.

[0026] The coating with waxy properties serves several functions: it protects the aloe vera plant parenchyma pieces from deterioration due to dehydration, and it forms a physical barrier against microorganisms, preventing them from adhering to the surface of the aloe vera plant parenchyma pieces and inhibiting microbial growth.

[0027] In addition, the presence of at least one tear tape facilitates the application of the cosmetic product. Specifically, the tear tape serves to remove a portion of the coating, leaving some of the solid portion uncovered and other portions still covered. The uncovered solid portion can be applied to any part of the user's body.

[0028] In a first aspect, the present invention refers to a single-dose cosmetic product comprising a solid portion of the parenchyma of the Aloe vera plant, a coating, and at least one tear tape, wherein the coating completely encapsulates the solid portion and is made of a material having waxy properties selected from the group consisting of hydrocarbon waxes and natural waxes or mixtures thereof, and the tear tape is made of a nonwoven material and is located between the coating and the solid portion.

[0029] Examples of natural waxes include carnauba wax, candelilla wax, beeswax, Japan wax, rice bran wax, and sunflower seed wax. Of the above waxes, beeswax is more preferred.

[0030] Examples of hydrocarbon waxes include polyethylene waxes, paraffin waxes, ceresin, and microcrystalline waxes.

[0031] Optionally, the cosmetic coating of the present invention further comprises natamycin to prevent fungal contamination of the product. Doses of 3 to 20 ppm may be used.

[0032] Optionally, the cosmetic coating of the present invention further comprises at least one synthetic or natural dye. Preferably, the dye is copper chlorophyllin.

[0033] In the present invention, the aloe vera parenchyma can be extracted from any aloe vera plant, including, for example, Aloe barbadensis Miller, Aloe Arborescens, Aloe Ferox, Aloe vera (L.) Burn, Aloe officinalis, and Aloe Vulgaris Lamarck. Preferably, the aloe vera plant is Aloe barbadensis Miller.

[0034] The tear tape is made of 100% nonwoven material. The material is a flat porous sheet produced directly from filaments. The material has properties that make it the best choice for its function, among them its tear strength and ability to adhere to coatings.

[0035] In another embodiment, the single dose cosmetic product of the present invention further comprises at least one cosmetic active ingredient selected from the group consisting of vitamins, minerals, essential oils and mixtures thereof inserted within the soft tissue.

[0036] The introduction of the at least one cosmetically active ingredient into the soft tissue can be achieved by simple diffusion via a molecular concentration gradient or by forced diffusion via injection into the soft tissue.

[0037] The active ingredients used in the cosmetic compositions of the single dose cosmetic of the present invention may have a wide variety of purposes and functions. In general, such ingredients provide, or are at least expected to provide, some benefit to the user, preferably to the user's skin or hair, such as anti-aging, cell regenerating, antioxidant, germicidal, moisturizing, healing effects, among others.

[0038] The amount of active ingredient used with the present invention can vary widely depending on the specific application, and all such amounts can be determined by those skilled in the art.For example, the amount of active ingredient can vary depending on the ability of the ingredient to penetrate through, on, or into the pores of the skin.Other factors that may affect the relative amount of active ingredient include the specific active ingredient involved, the specific benefit desired, the user's sensitivity to the active ingredient, the user's health, age, skin condition, etc.In summary, active ingredient is preferably used in a "safe and effective amount", which is an amount that is large enough to bring about the desired effect on the skin.

[0039] In a second aspect of the present invention, there is provided a method for producing a single-dose cosmetic product according to the first aspect of the present invention, the method comprising the steps of: (i) Cleaning and disinfecting the surface of the aloe vera leaves; (ii) removing the cuticle and outer skin of Aloe vera leaves to obtain the parenchyma of Aloe vera; (iii) cutting the parenchyma of Aloe vera into solid pieces and immersing the solid pieces in a solution of a processing aid; (iv) removing the solution of processing aids from the part by filtration; (v) attaching to each of the solid portions at least one tear tape, the tear tape being a nonwoven material; (vi) covering the portion having the tear tape of step (v) with one made of a material having waxy properties selected from the group consisting of hydrocarbon waxes and natural waxes or mixtures thereof; and (vii) subjecting the product obtained in step (vi) to high pressure treatment to obtain a cosmetic product, wherein the pressure is 400 MPa to 600 MPa.

[0040] In the present invention, "processing aids" are auxiliary technical substances that are indirectly added for manufacturing purposes. These substances are added to the cosmetic product during the manufacturing process to keep the raw materials stable during the manufacturing process, and are removed from the cosmetic product before it is packaged in its final form according to good manufacturing practice. Those skilled in the art should know how to select said processing aids for this application.

[0041] In another embodiment, when the single-dose cosmetic product of the first aspect of the present invention further comprises at least one cosmetic active, it has an additional step prior to step (iv) consisting of inserting at least one cosmetic active selected from the group consisting of inserted vitamins, minerals, essential oils and mixtures thereof into the soft tissue.

[0042] The introduction of the at least one cosmetically active ingredient into the soft tissue can be achieved by simple diffusion via a molecular concentration gradient or by forced diffusion via injection into the soft tissue.

[0043] In the method of the present invention, the time for the high pressure treatment in step (vii) is preferably 3 to 6 minutes, and more preferably 3 minutes.

[0044] Optionally, the process comprises the step (viii) of packaging the product obtained in step (vii) in a stand-up pouch or a sealed sachet.

[0045] [Brief description of the drawings] BRIEF DESCRIPTION OF THE DRAWINGS The drawings are described below and are intended to illustrate exemplary embodiments and not to limit the scope of the invention. Figure 1 shows an unopened cosmetic product. Figure 2 shows the cosmetic product in the open state. FIG. 3 shows the cosmetic product opened with a portion of the coating removed to expose a portion of the solid portion of the soft tissue for application. FIG. 4 shows the percent loss of each active compound (acemannan (alobelloses), glucomannan, mannose, and vitamins A and C) relative to the initial concentration for each of the analyzed Aloe vera forms (the cosmetic products of the present invention, i.e., Aloe vera gel and Aloe vera cream) after 10 months of storage.

[0046] [Description of the embodiment] Example 1. Manufacturing method of the cosmetic product of the present invention First of all, it is not necessary to go into extensive detail here since a person skilled in the art would know suitable devices and equipment for carrying out the steps according to the present invention and would select a suitable system based on professional experience.

[0047] First, the coarse dirt on the surface of the Aloe vera leaves was removed by immersing them in a water immersion bath.Then, the Aloe vera bark was disinfected by immersing the Aloe vera leaves in a 1:3 ratio of water and sodium hypochlorite for 5 minutes.Then, the Aloe vera leaves were immersed in a water bath for 3 minutes and washed with water.

[0048] The outer bark of the Aloe vera leaves was then mechanically brushed with an industrial washing machine and then rinsed with a pressurized water jet. The top and bottom of the Aloe vera leaves were trimmed with a cutting blade and then rinsed with a pressurized water jet.

[0049] Then, the Aloe vera leaves were peeled, the cuticle and outer skin of the leaves were removed, and the leaves were cut into partial pieces (approximately 5x2.5x1.5 cm) using a shear blade or a high-pressure water jet device to obtain Aloe vera parenchyma strips.

[0050] Cut pieces of Aloe vera parenchyma were subjected to industrial blanching in boiling water (100°C) for 30-60 seconds. The pieces were then immersed in a solution of processing aids and the cut pieces were subsequently stored for 18-20 hours. This immersion ensured that the Aloe vera parenchyma pieces did not lose their physical properties such as color and texture and prevented contamination during the storage period.

[0051] In this example, the processing aid used was a composition containing sorbate (0.2-0.4% w / w), benzoate (0.05-0.1% w / w), ascorbic acid (0.1-0.2% w / w), L-cysteine ​​(0.3-0.6% w / w), water (99.35-98.3% w / w) and citric acid (maximum pH 3.4).

[0052] Then, the technical auxiliary solution mentioned in the previous step was completely removed by gravity filtration using a perforated mesh for 10 minutes. When the technical auxiliary solution was removed, the pieces were separated from each other.

[0053] A tear tape of a nonwoven material was applied to each piece at the center of the piece along the length and width of the piece. The tear tape was not secured by any means, it was simply applied by hand to the surface of the piece.

[0054] The area with the tear tape was then covered with a coating having waxy properties. In this example, the coating composition was 70%-80% ester, 10%-15% free acid, and 10%-20% paraffin.

[0055] The coating was heated at temperatures between 75 and 100°C for 2 to 4 hours until it was completely melted and liquid. The liquid coating was applied to the parts, followed by a cooling stage using a cold tunnel system (below 14°C).

[0056] Finally, the product obtained in the previous step was subjected to high pressure treatment to obtain the cosmetic product of the present invention. The pressure of the high pressure treatment was 600 MPa for 3 minutes.

[0057] The resulting product is shown in Figure 1. For use, the user pulls the tear tape, partially peeling back the coating and exposing a portion of the solid portion of the soft tissue (Figures 2 and 3). The user can then apply the solid portion of the soft tissue to the desired area.

[0058] Example 2. Comparative test of the properties of the cosmetic product of the present invention and a commercially available product containing aloe vera The beneficial effects of Aloe vera are directly related to the active compounds it contains. The methods used to preserve and stabilize the products obtained from Aloe vera can have a significant impact on the concentration of these compounds and therefore on the strength of the beneficial effects attributed to them.

[0059] For this purpose, four samples of different forms of Aloe vera were analyzed by high performance liquid chromatography (HPLC). Table 1 shows the composition of each sample.

[0060] Each sample was analyzed for the concentration of the main compounds in Aloe vera: acemannan (allovelose), glucomannan, mannose, as well as vitamins A and C. Table 2 shows the results obtained.

[0061] [Table 1]

[0062] [Table 2]

[0063] Table 3 shows the percentage of each compound in each type of sample relative to the concentration in pure Aloe vera (sample 1).

[0064] [Table 3]

[0065] The cosmetic product of the present invention shows that the concentrations of each of the analyzed compounds are closer to that of the pure Aloe vera product (sample 1). In particular, the concentrations of polysaccharides (acemannan and glucomannan) and the monosaccharide mannose are similar to those of the natural product, i.e., these compounds remain intact and are not adversely affected by the treatment. Vitamin C is slightly degraded during the treatment, but maintains a concentration of 92.7% compared to pure Aloe vera (sample 1). On the other hand, the slightly elevated concentration of Vitamin A (less than 100%) in sample 2 may be due to heterogeneity between the samples, but in any case, it indicates that Vitamin A is not degraded during the treatment.

[0066] Aloe vera gel (sample 3) shows a reduction of approximately 10% in sugars, 23.5% in vitamin C, and 16% in vitamin A. In the cream form (sample 4), none of the compounds are present in concentrations greater than 15% compared to pure Aloe vera (sample 1).

[0067] Furthermore, the stability of the cosmetic product of the present invention was evaluated and its shelf life and degradation over time were estimated in comparison with commercial products.

[0068] Samples 2, 3, and 4 were stored in a temperature-controlled chamber (20°C) and monitored over time. Samples were analyzed at the start of the study and then periodically every 2 months up to 10 months. The main compounds in Aloe vera mentioned above were used for shelf-life monitoring. The analytical method was by high performance liquid chromatography (HPLC). The results obtained are shown in Tables 4, 5, and 6, and in Figure 4.

[0069] [Table 4]

[0070] [Table 5]

[0071] [Table 6]

[0072] As shown in the table above, the initial concentrations of the analyzed compounds were higher in the cosmetic product of the present invention compared to the other two products (gel and cream), and mainly compared to the Aloe Vera cream, the concentrations were about 10 times higher in the cosmetic product of the present invention.

[0073] Furthermore, after 10 months of storage, the product of the present invention did not have any significant loss of any of the analyzed compounds, considering that the difference between the initial and final concentrations of the analyzed compounds was lower than the analytical uncertainty (the loss rate was not significant) (see Figure 4).

[0074] In contrast, the gel experienced significant losses of vitamin A (11.7%), vitamin C (10.7%), and allobenose (11.35%), as did the cream, which experienced losses of vitamin C (18.9%), vitamin A (7.88%), and mannose (6.5%).

[0075] The conclusion of the tests carried out is that the concentrations of both analyzed compounds (the active ingredients that give rise to the health-promoting properties of Aloe Vera) are higher in the cosmetic product of the invention than in the gel and significantly higher (about 10 times higher) than in the Aloe Vera cream.

[0076] Moreover, the cosmetic product of the present invention maintains the same beneficial properties as pure aloe vera. Moreover, because the cosmetic product of the present invention is provided in a single dose form and has been subjected to high pressure processing (a physical process that preserves the original properties of the product), it does not require the use of preservatives, unlike other forms.

[0077] Another advantage of the products of the present invention is that they are less allergenic than other forms (gels and creams) since they do not contain preservatives that may cause allergic reactions upon ingestion or contact.

[0078] Example 3. Metagenomic analysis of the cosmetic product of the present invention DNA extraction from samples of the products of the present invention was performed using two commercially available DNA extraction kits: (1) DNeasy UltraClean Microbial kit (Qiagen) and (2) DNeasy PowerLyzer PowerSoil kit (Qiagen).

[0079] The resulting DNA was quantified using Qubit (Thermofisher).

[0080] The samples with the highest DNA content were subjected to amplification of the 16S rRNA gene, which is conserved in all bacterial species and allows for identifying and establishing lineages between different bacterial species based on differences in the genetic sequence of this gene.

[0081] As a result, the samples of the cosmetic product of the present invention contained bacteria from the following five genera: Pantoea, Yersinia, Rahnella, Erwinia, and Serratia. More specifically, the main species present are Pantoea agglomerans, Yersinia kristensenii, Erwinia billingiae, Rahnella Victoriana, Pantoea allii, Serratia liquefaciens, Yersinia ruckeri, Rahnella woolbedingensis, Leclercia adecarboxylata, Serratia grimesii, Rahnella inusitata and Pantoea ananatis.

[0082] These endophytic microorganisms are natural probiotics present in plant tissues (parenchyma).By keeping the parenchyma intact, the products of the present invention keep these microorganisms alive, resulting in healthy benefits to the skin.

[0083] In conventional cosmetics, whether in the form of gels, creams or ointments, the production of these products requires the "cutting" or "destroying" of the tissue of the aloe plant in which the microorganisms live, making it impossible to keep these microorganisms alive through the production process. [Brief description of the drawings]

[0084] [Figure 1] Figure 1 shows an unopened cosmetic product. [Diagram 2] Figure 2 shows the cosmetic product in the process of being opened. [Diagram 3] FIG. 3 shows the cosmetic product opened with a portion of the coating removed to expose a portion of the solid portion of the soft tissue for application. [Figure 4] FIG. 4 shows the percent loss of each active compound (acemannan (alobelloses), glucomannan, mannose, and vitamins A and C) relative to the initial concentration for each of the analyzed Aloe vera forms (the cosmetic products of the present invention, i.e., Aloe vera gel and Aloe vera cream) after 10 months of storage.

Claims

1. A single-dose cosmetic product (1) comprising a solid portion of the soft tissue of the aloe vera plant (2), a coating (3), and at least one tear tape (4), said coating (3) completely encapsulating said solid portion (2) and made of a material having waxy properties selected from the group consisting of hydrocarbon waxes and natural waxes or mixtures thereof; and The single-dose cosmetic product, wherein the tear tape (4) is made of a nonwoven material and is disposed between the coating and the solid portion.

2. 2. The single-dose cosmetic product of claim 1, wherein the coating (3) further comprises natamycin.

3. 10. The single-dose cosmetic product of claim 1, wherein the coating (3) further comprises at least one synthetic or natural dye.

4. 10. The single-dose cosmetic product of claim 1, wherein the cosmetic product (1) further comprises at least one cosmetic active ingredient selected from the group consisting of vitamins, minerals, essential oils and mixtures thereof, inserted within the solid portion of the soft tissue (2).

5. A method for producing the single-dose cosmetic product (1) according to claim 1, comprising the steps of: (i) cleaning and disinfecting the surface of the aloe vera leaves; (ii) removing the cuticle and outer skin of the Aloe vera leaves to obtain the Aloe vera parenchyma; (iii) cutting the aloe vera parenchyma into solid portions (2) and immersing the solid portions (2) in a solution of a processing aid; (iv) removing the solution of processing aid from the portion by filtration; (v) attaching to each of said solid portions (2) at least one tear tape (4) of nonwoven material; (vi) covering said solid part (2) having the tear tape (4) of step (v) with a coating made of a material having waxy properties selected from the group consisting of hydrocarbon waxes and natural waxes or mixtures thereof; and (vii) subjecting the product obtained in step (vi) to high pressure treatment to obtain the cosmetic product (1), wherein the pressure is 400 MPa to 600 MPa.

6. 6. The method of claim 5, further comprising the additional step of inserting at least one cosmetic active ingredient selected from the group consisting of vitamins, minerals, essential oils and mixtures thereof into the soft tissue prior to step (v).

7. The method according to claim 5, wherein the pressure of the high pressure treatment in step (vii) is 600 MPa.

8. The method according to claim 5, wherein the high pressure treatment in step (vii) lasts for 3 to 6 minutes.

9. The method according to claim 8, wherein the duration of the high pressure treatment in step (vii) is 3 minutes.

10. 6. The process according to claim 5, wherein the process further comprises the step (viii) of packaging the product obtained in step (vii) in a stand-up pouch or a sealed sachet.

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