Compositions of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart and their use in resistance to atherosclerosis

Abstract

A composition of Mars Needsvechkhana Dick and Prunus serasifera Ehrhart, wherein the weight ratio of Mars Needsvechkhana Dick to Prunus serasifera Ehrhart is 1:10 to 10:1. The composition has a synergistic effect in the prevention and / or treatment of atherosclerosis. The product forms of the composition of Mars Needsvechkhana Dick and Prunus serasifera Ehrhart include fruit juice, jam, fruit powder, oral liquid products, capsules, tablets, etc.

Description

Technical Field

[0001] The present invention relates to the fields of food and pharmaceuticals, specifically, compositions of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart, and their use in resisting atherosclerosis.

Background Art

[0002] Atherosclerosis (As) is an inflammatory disease involving the arterial wall characterized by the accumulation of lipids and inflammatory cells in the intima of large arteries. Atherosclerosis can lead to atherosclerotic cardiovascular disease (ASCVD). Clinically diagnosed ASCVD diseases mainly include acute coronary syndrome, a history of myocardial infarction, stable or unstable angina, coronary revascularization (including interventional therapy and bypass surgery), other peripheral arterial diseases or postoperative revascularization, atherosclerotic stroke or transient ischemic attack. At present, ASCVD is the most common cause of death worldwide, posing a serious risk to health. The incidence of atherosclerosis in China is clearly on the rise, and it is often seen in middle-aged and elderly people. Surveys have shown that by 2020, 2 billion people worldwide will have atherosclerotic carotid artery disease. A survey including 85,000 people in China showed that one-third of the elderly have atherosclerosis.

[0003] Clinical drugs for treating atherosclerosis mainly include: (1) lipid-lowering agents: statins, ezetimibe, PCSK9 inhibitors, etc.; (2) antiplatelet agents: aspirin, clopidogrel, etc. The pathogenesis of AS is complex. Many studies have shown that low-density lipoprotein cholesterol (LDL-C) plays an important role in the occurrence and development of AS, and lipid-lowering agents are preferred drugs. Furthermore, there are also oxidative stress theory and inflammation theory regarding the pathogenesis of atherosclerosis. At present, for example, there are some literatures or clinical trials on colchicine and canakinumab regarding the inflammation theory, and probucol and idebenone regarding oxidative stress. There are some Chinese patent medicines in the clinical treatment of atherosclerosis resistance, such as Compound Danshen dropping pills, Qishen Yiqi dropping pills, Naoxintong capsules, etc. Atherosclerosis requires a treatment regimen under the guidance of a doctor and long-term medication. Different drugs have different degrees of side effects on the human body, such as liver and muscle damage, gastrointestinal reactions, etc., so a more natural and safe method of the present invention is needed.

[0004] Malus niedzwetzkyana Dieck is a plant of the genus Malus Mill in the Rosaceae family. Its fresh fruit is red and has very high utilization value in terms of germplasm source research, processing and utilization. Malus niedzwetzkyana Dieck has multiple varieties, and there are significant differences in the size and color of the fruits. China, New Zealand, Japan, Switzerland and other countries have carried out breeding of Malus niedzwetzkyana Dieck. Specifically, it includes Hongxun series in Xinjiang, China, Robert Crab in the United States, Ruby Sweet in Japan, Redlove in Switzerland, etc. Malus niedzwetzkyana Dieck in Xinjiang plays an important role in the origin of fresh red apple plants.

[0005] Prunus cerasifera Ehrhart is generally called wild plum and is a plant of the genus Prunus in the Rosaceae family. Wild Prunus cerasifera Ehrhart is listed as the second-class national key protected species. It is a wild fruit tree seed source that is rare worldwide and of endangered origin. It is rarely distributed naturally around the world. Daxigou and Xiaoxigou in the lli Valley of Xinjiang are the largest concentrated distribution ranges of wild Prunus cerasifera Ehrhart in the world. The fruits of Prunus cerasifera Ehrhart have a long history of consumption in Xinjiang, China. Kazak and Mongolian herdsmen living in Daxigou in the Tianshan Mountains mainly eat beef and mutton, but there are few people suffering from dyslipidemia and hypertension. This is related to the long-term usage habits of herdsmen of picking wild Prunus cerasifera Ehrhart to make jam or drying the fruits to make black tea. Prunus cerasifera Ehrhart includes multiple varieties and is mainly classified into black fruits, red fruits, purple fruits, dark purple fruits, yellow fruits, etc. according to color.

[0006] Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart are rich in anthocyanins, polyphenols, flavonoids and other functional components and have high development value. However, current research is still limited, mainly focusing on chemical components and oxidation resistance, and is still in the preliminary research stage.

Summary of the Invention

[0007] After many experimental studies, the inventors of the present invention found that the combination of Malus niedzwetzkyana Dieck (or an extract of Malus niedzwetzkyana Dieck) and Prunus cerasifera Ehrhart (or an extract of Prunus cerasifera Ehrhart) shows a synergistic effect on resistance to atherosclerosis.

[0008] Therefore, in a first aspect, the present invention provides A first component that is Mars Needs Vegetable Canard or an extract of Mars Needs Vegetable Canard; and A second component that is Prunus cerasifera Ehrhart or an extract of Prunus cerasifera Ehrhart to provide a composition comprising the same.

[0009] In some embodiments, the weight ratio of the first component to the second component is from 1:10 to 10:1 (e.g., 1:9 to 10:1, 1:8 to 10:1, 1:7 to 10:1, 1:6 to 10:1, 1:5 to 10:1, 1:4 to 10:1, 1:3 to 10:1, 1:2 to 10:1, 1:1 to 10:1, 1:10 to 9:1, 1:9 to 9:1, 1:8 to 9:1, 1:7 to 9:1, 1:6 to 9:1, 1:5 to 9:1, 1:4 to 9:1, 1:3 to 9:1, 1:2 to 9:1, 1:1 to 9:1, 1:10 to 8:1, 1:9 to 8:1, 1:8 to 8:1, 1:7 to 8:1, 1:6 to 8:1, 1:5 to 8:1, 1:4 to 8:1, 1:3 to 8:1, 1:2 to 8:1, 1:1 to 8:1, 1:10 to 7:1, 1:9 to 7:1, 1:8 to 7:1, 1:7 to 7:1, 1:6 to 7:1, 1:5 to 7:1, 1:4 to 7:1, 1:3 to 7:1, 1:2 to 7:1, 1:1 to 7:1, 1:10 to 6:1, 1:9 to 6:1, 1:8 to 6:1, 1:7 to 6:1, 1:6 to 6:1, 1:5 to 6:1, 1:4 to 6:1, 1:3 to 6:1, 1:2 to 6:1, 1:1 to 6:1, 1:10 to 5:1, 1:9 to 5:1, 1:8 to 5:1, 1:7 to 5:1, 1:6 to 5:1, 1:5 to 5:1, 1:4 to 5:1, 1:3 to 5:1, 1:2 to 5:1, 1:1 to 5:1, 1:10 to 4:1, 1:9 to 4:1, 1:8 to 4:1, 1:7 to 4:1, 1:6 to 4:1, 1:5 to 4:1, 1:4 to 4:1, 1:3 to 4:1, 1:2 to 4:1, 1:1 to 4:1, 1:10 to 3:1, 1:9 to 3:1, 1:8 to 3:1, 1:7 to 3:1, 1:6 to 3:1, 1:5 to 3:1, 1:4 to 3:1, 1:3 to 3:1, 1:2 to 3:1, 1:1 to 3:1, 1:10 to 2:1, 1:9 to 2:1, 1:8 to 2:1, 1:7 to 2:1, 1:6 to 2:1, 1:5 to 2:1, 1:4 to 2:1, 1:3 to 2:1, 1:2 to 2:1, 1:1 to 2:1, 1:10 to 1:1, 1:9 to 1:1, 1:8 to 1:1, 1:7 to 1:1, 1:6 to 1:1, 1:5 to 1:1, 1:4 to 1:1, 1:3 to 1:1, 1:2 to 1:1, 1:10 to 5:1, 1:10 to 2:1, 1:10 to 1:1, 1:5 to 10:1, 1:5 to 5:1, 1:5 to 2:1, 1:5 to 1:1, 1:2 to 10:1, 1:2 to 5:1, 1:2 to 2:1, 1:2 to 1:1, 1:1 to 10:1, 1:1 to 5:1, 1:1 to 2:1, for example, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2,1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1).

[0010] In some embodiments, the Malus domestica Borkh or an extract of Malus domestica Borkh is obtained from / by fresh fruit, fresh fruit pulping or juice extraction, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods.

[0011] In some embodiments, the Prunus cerasifera Ehrhart or an extract of Prunus cerasifera Ehrhart is obtained from / by fresh fruit, fresh fruit pulping or juice extraction, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods.

[0012] In some embodiments, the composition is in the form of fresh fruit, fruit juice, jam, powder (e.g., freeze-dried powder), candy (e.g., tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, wine, or pastry. In other words, the product form of the composition can be fresh fruit, fruit juice, jam, powder (e.g., freeze-dried powder), candy (e.g., tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, wine, or pastry. That is, the composition can be made into fresh fruit, fruit juice, jam, powder (e.g., freeze-dried powder), candy (e.g., tablet candy), oral liquid, capsule, tablet, dried fruit, preserved fruit, wine, or pastry. Among them, fruit juice, jam, powder (e.g., freeze-dried powder), candy (e.g., tablet candy), oral liquid, capsule, tablet can be prepared from the extracts obtained by leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods.

[0013] In some embodiments, the composition is in the form of a medicament (e.g., traditional Chinese medicine, Chinese patented medicine, natural medicine, etc.), a normal food, a functional food, or a health food. In other words, the composition can be made into a medicament (e.g., traditional Chinese medicine, Chinese patented medicine, natural medicine, etc.), a normal food, a functional food, or a health food.

[0014] In some embodiments, Malus niedzwetzkyana Dieck refers to the fruit of a plant of the genus Malus Mill of Rosaceae that is red and fresh.

[0015] In some embodiments, Malus niedzwetzkyana Dieck is selected from Hongxun 1, Hongxun 3, Hongxun 4, Hongxun 6, Hongxun 8, Red Love, etc.

[0016] In some embodiments, Prunus cerasifera Ehrhart is selected from the red fruit of Prunus cerasifera Ehrhart, the black fruit of Prunus cerasifera Ehrhart, the purple fruit of Prunus cerasifera Ehrhart, the dark purple fruit of Prunus cerasifera Ehrhart, the yellow fruit of Prunus cerasifera Ehrhart, etc.

[0017] In a second aspect, the present invention provides a first product, which is the aforementioned composition; a second product, which is a medicament for preventing and / or treating atherosclerotic diseases and provides a combination product comprising the same.

[0018] In some embodiments, the atherosclerotic disease is selected from atherosclerosis of the aorta and its major branches, coronary atherosclerosis, cerebral atherosclerosis, renal atherosclerosis, mesenteric atherosclerosis, peripheral atherosclerosis, etc.

[0019] In some embodiments, the agent for preventing and / or treating atherosclerotic diseases is selected from statins (e.g., rosuvastatin, atorvastatin, simvastatin), ezetimibe, PCSK9 inhibitors, aspirin, clopidogrel, colchicine, connamab, probucol, idebenone, etc.

[0020] In some embodiments, the weight ratio of the first product to the second product is from 1000:0.001 to 1000:33.4 (e.g., 1000:0.002075, 1000:0.004175, 1000:0.0083, 1000:0.02075, 1000:0.04175, 1000:0.083, 1000:0.08325, 1000:0.167, 1000:0.20875, 1000:0.3125, 1000:0.333, 1000:0.4175, 1000:0.62625, 1000:0.835, 1000:1.25, 1000:1.67, 1000:2.0875, 1000:2.505, 1000:2.875, 1000:4.175, 1000:8.35, or 1000:16.7, or, for example, from 1000:0.002075 to 1000:16.7).

[0021] In some embodiments, when the second product is rosuvastatin, the weight ratio of the first product to the second product is from 1000:0.01 to 1000:0.34 (e.g., 1000:0.02075, 1000:0.04175, 1000:0.083 or 1000:0.167, or, for example, from 1000:0.02075 to 1000:0.167).

[0022] In some embodiments, when the second product is atorvastatin, the weight ratio of the first product to the second product is from 1000:0.02 to 1000:0.67 (e.g., 1000:0.04175, 1000:0.08325, 1000:0.167 or 1000:0.333, or, for example, from 1000:0.04175 to 1000:0.333).

[0023] In some embodiments, when the second product is aspirin, the weight ratio of the first product to the second product is from 1000:0.10 to 1000:5.01 (for example, 1000:0.20875, 1000:0.4175, 1000:0.62625, 1000:0.835, 1000:1.67, or 1000:2.505, or, for example, from 1000:0.20875 to 1000:2.505).

[0024] In some embodiments, when the second product is clopidogrel, the weight ratio of the first product to the second product is from 1000:0.10 to 1000:5.01 (for example, 1000:0.20875, 1000:0.4175, 1000:0.62625, 1000:0.835, 1000:1.67, or 1000:2.505, or, for example, from 1000:0.20875 to 1000:2.505).

[0025] In some embodiments, when the second product is colchicine, the weight ratio of the first product to the second product is from 1000:0.001 to 1000:0.166 (for example, 1000:0.002075, 1000:0.004175, 1000:0.0083, 1000:0.02075, or 1000:0.083, or, for example, from 1000:0.002075 to 1000:0.083).

[0026] In some embodiments, when the second product is probucol, the weight ratio of the first product to the second product is from 1000:1.0 to 1000:33.4 (for example, 1000:2.0875, 1000:2.875, 1000:4.175, 1000:8.35, or 1000:16.7, or, for example, from 1000:2.0875 to 1000:16.7).

[0027] In a third aspect, the present invention provides the use of the aforementioned composition in the preparation of a medicament (e.g., traditional Chinese medicine, Chinese patented medicine, natural medicine, etc.), a normal food, a functional food or a health food for preventing and / or treating atherosclerotic diseases. The present invention also provides the use of the aforementioned combined product in the preparation of a medicament (e.g., traditional Chinese medicine, Chinese patented medicine, natural medicine, etc.) for preventing and / or treating atherosclerotic diseases.

Embodiments for Carrying Out the Invention

[0028] In the present invention, a normal food refers to a finished product and raw materials for human diet, and items that are traditionally both food and medicine but do not include items for therapeutic purposes.

[0029] In the present invention, a health food refers to a food that is required to have a specific health function or is intended to supplement vitamins and minerals, that is, a food that is suitable for a specific human, has a function of controlling the human body, is not intended to treat diseases, and does not cause any acute, subacute, or chronic injury to the human body. A functional food is mainly a name for foods in Europe and Japan that can improve the health status of the body or reduce diseases, and is similar to the definition of health food in China.

[0030] In the present invention, traditional Chinese medicine is a medicament used to guide collection, processing and preparation, explain the mechanism of action, and guide clinical application according to traditional Chinese medicine theory. Traditional Chinese medicine mainly comes from natural medicines and their processed products, including herbal medicines, animal medicines, mineral medicines, and some chemical and biological products, most of which are herbal medicines.

[0031] In the present invention, a Chinese patented medicine prepared into a certain dosage form of Chinese medicine product for preventing and treating diseases according to a specific prescription and preparation process under the guidance of traditional Chinese medicine theory is a commercially available Chinese medicine approved by the National Medical Products Administration.

[0032] In the present invention, natural drugs refer to animal drugs, plant drugs, mineral drugs, etc. that are known to have certain pharmacological activities under the modern medical system. Natural drugs are not equivalent to traditional Chinese medicine or Kampo medicine. Natural drugs refer to natural drug substances and their preparations used under the guidance of modern medical theory. Their origins include plants, animals, and minerals, and usually exclude substances derived from genetically modified animals and plants, substances subjected to microbial fermentation or chemical modification, etc.

[0033] The present invention also provides a combined product comprising a first product and a second product packaged separately, wherein the first product is a combined product of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart, and the second product is an anti-atherosclerotic drug (for example, lipid-lowering agent, antiplatelet agent, anticoagulant, anti-inflammatory agent, antioxidant stress agent, etc.).

[0034] In the present invention, "individual" includes humans or non-human animals. Exemplary human individuals include human individuals (referred to as patients) having a disease (for example, the diseases described herein) or normal individuals. "Non-human animals" in the present invention include all vertebrates, for example, non-mammals (for example, fish, birds, amphibians, reptiles) and mammals, for example, non-human primates, livestock and / or breeding animals (for example, rats, sheep, dogs, cats, cows, pigs, etc.).

[0035] In the present invention, "extract of Prunus cerasifera Ehrhart" or "extract of Malus niedzwetzkyana Dieck" refers to a product formed by subjecting Prunus cerasifera Ehrhart or Malus niedzwetzkyana Dieck as raw materials to physical and / or chemical extraction and separation processes, thereby obtaining and concentrating one or more active ingredients in Prunus cerasifera Ehrhart or Malus niedzwetzkyana Dieck, and can be used as a raw material or adjuvant for foods (for example, ordinary foods or functional foods or health foods) or pharmaceuticals. Exemplary extraction methods include, but are not limited to, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation, etc.

[0036] Exemplary methods for preparing extracts of Mars Needs Vegetables Dick and Prunus Seraphifera Heart are provided below.

[0037] Leaching extraction In some embodiments, the extracts of Mars Needs Vegetables Dick and Prunus Seraphifera Heart of the present invention are obtained by leaching extraction (e.g., water leaching extraction or alcohol leaching extraction).

[0038] In some embodiments, the leaching extraction is Step 1: Remove the seeds or cores of Mars Needs Vegetables Dick and Prunus Seraphifera Heart respectively, and take the pulp directly to obtain the pulp, or separate the juice from the squeezed residue; in some embodiments, the juice and the squeezed residue are separated by filtration using gauze (e.g., 100 - 300 mesh gauze, e.g., 200 mesh gauze); Step 2: Mix the pulp or the squeezed residue with an extraction medium (e.g., water or ethanol), add cellulase, extract at room temperature or under heating (e.g., 10 - 100 °C, e.g., 50 °C) (e.g., 30 - 100 minutes, e.g., 60 minutes), and filter to obtain the pulp or squeezed residue extract; in some embodiments, the pulp or the squeezed residue is mixed with the extraction medium at a weight ratio of 1:(2 - 10) (e.g., 1:5); in some embodiments, the ethanol is 20% - 100% (e.g., 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) ethanol; Step 3: Concentrate the combined solution of the pulp extract or the combination of the juice and the squeezed residue extract to obtain the leaching extract of Mars Needs Vegetables Dick and Prunus Seraphifera Heart; in some embodiments, the concentration is carried out by rotary evaporation; in some embodiments, the rotary evaporation temperature is 30 - 60 °C, e.g., 50 °C; Step 4: Mixing the extracts of Mars neesvecana Deek and Prunus serasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, and 10:1) to prepare the composition, or first, mixing the raw materials of Mars neesvecana Deek and Prunus serasifera Ehrhart in the aforementioned different ratios in Step 1, and then extracting including the step of

[0039] In some embodiments, the extracts of Mars neesvecana Deek and Prunus serasifera Ehrhart of the present invention are obtained by water leaching extraction. In some embodiments Step 1: Removing the seeds or cores of Mars neesvecana Deek and Prunus serasifera Ehrhart respectively, taking the pulp directly to obtain the pulp, or filtering through a 100 - 300 mesh gauze to obtain the fruit juices of Mars neesvecana Deek and Prunus serasifera Ehrhart, thereby separating the juice from the pomace; In some embodiments, the gauze has a mesh number of 200 meshes; Step 2: Mixing the pulp or pomace of Mars neesvecana Deek and Prunus serasifera Ehrhart with water at a feed - to - liquid ratio of 1:(2 - 10), adding cellulase, extracting at 10 - 100 °C for 30 - 100 minutes, and filtering to obtain the pulp or pomace extract; In some embodiments, the feed - to - liquid ratio is selected to be 1:5; In some embodiments, the leaching extraction temperature is selected to be 50 °C; In some embodiments, the extraction time is selected to be 60 minutes; In some embodiments, cellulase is added in an amount in the range of 0.2% - 1% (e.g., 0.5%) based on the weight of the pomace or pulp; Step 3: Concentrate the solution of the pulp extract of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart, or a combination of fruit juice and squeezed residue extract, by rotary evaporation at 30 - 60°C to obtain the water leaching extract of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart; in some embodiments, the rotary evaporation temperature is selected to be 50°C; Step 4: Mix the water leaching extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or first mix the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, and then extract. This includes the step of

[0040] In some embodiments, the extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart of the present invention are obtained by alcohol leaching extraction. In some embodiments, Step 1: Remove the seeds or cores of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart respectively, and take the pulp directly to obtain the pulp, or filter through a 100 - 300 mesh gauze to obtain the fruit juice of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart, thereby separating the fruit juice from the squeezed residue; in some embodiments, the gauze has a mesh count of 200 meshes; Step 2: Mix the pulp or squeezed residue of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart with ethanol at a feed-to-liquid ratio of 1:(2 - 10) by 20 - 100% (e.g., 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%); add 0.2% - 1% (based on the weight of the squeezed residue or pulp) (e.g., 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%) of cellulase; extract at 10 - 100 °C for 30 - 100 minutes; and filter to obtain the pulp or squeezed residue extract. In some embodiments, the feed-to-liquid ratio is selected to be 1:5. In some embodiments, the concentration of ethanol used is 40%. In some embodiments, the leaching extraction temperature is selected to be 50 °C. In some embodiments, the extraction time is selected to be 60 minutes. Step 3: Concentrate the combined solution of the pulp extract of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart, or the combination of fruit juice and squeezed residue extract, by rotary evaporation at 30 - 60 °C, directly concentrate the pulp extract to obtain the alcohol leaching extract of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart. In some embodiments, the rotary evaporation temperature is selected to be 50 °C. Optionally, the extract is evaporated until dry to remove the residual ethanol. Step 4: Mix the alcohol leaching extracts of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or first mix the raw materials of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart in the aforementioned different ratios in Step 1 and then extract. includes the steps of

[0041] Ultrasonic extraction In some embodiments, the extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart of the present invention are obtained by ultrasonic extraction (for example, ultrasonic water extraction or ultrasonic alcohol extraction).

[0042] In some embodiments, the ultrasonic extraction is Step 1: Remove the seeds or cores of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart respectively, and take the pulp directly to obtain the pulp, or separate the juice from the squeezed residue; in some embodiments, the juice and the squeezed residue are separated by filtration using gauze (for example, 100 - 300 mesh gauze, for example, 200 mesh gauze); Step 2: Mix the pulp or the squeezed residue with an extraction medium (for example, water or ethanol) and cellulase, extract under heating (for example, 30 - 50 °C, for example, 45 °C) (for example, 30 - 100 minutes, for example, 60 minutes), treat under ultrasonic conditions (for example, ultrasonic power of 100 - 500 W) (for example, 20 - 60 minutes, for example, 30 minutes), and filter to obtain the pulp or squeezed residue extract; in some embodiments, cellulase is added in an amount in the range of 0.2% - 1% (for example, 0.5%) based on the weight of the squeezed residue or the pulp; in some embodiments, the pulp or the squeezed residue and the extraction medium are mixed at a weight ratio of 1:(2 - 10) (for example, 1:5); in some embodiments, the ethanol is 20% - 100% (for example, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%) ethanol; Step 3: Concentrate the combined solution of the pulp extract of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart or the combination of the juice and the squeezed residue extract to obtain the ultrasonic extract of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart; in some embodiments, the concentration is carried out by rotary evaporation; in some embodiments, the rotary evaporation temperature is 30 - 60 °C, for example, 50 °C; Step 4: Mixing the ultrasonic extracts of Mars neesvecana Deicke and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, and 10:1) to prepare a composition, or first, mixing the raw materials of Mars neesvecana Deicke and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, and then extracting including the steps of.

[0043] In some embodiments, the extracts of Mars neesvecana Deicke and Prunus cerasifera Ehrhart of the present invention are obtained by ultrasonic water extraction. In some embodiments, Step 1: Removing the seeds or cores of Mars neesvecana Deicke and Prunus cerasifera Ehrhart respectively, taking the pulp directly to obtain the pulp, or filtering through a 100 - 300 mesh gauze to obtain the fruit juices of Mars neesvecana Deicke and Prunus cerasifera Ehrhart, thereby separating the juice from the squeezed residue; in some embodiments, the gauze has a mesh count of 200 meshes; Step 2: Mixing the pulp or squeezed residue of Mars neesvecana Deicke and Prunus cerasifera Ehrhart with water at a feed - to - liquid ratio of 1:(2 - 10), adding cellulase at 0.2% - 1% (based on the weight of the squeezed residue or pulp), extracting in a water bath at 30 - 50°C for 30 - 100 minutes, treating with ultrasonic waves at 100 - 500W for 20 - 60 minutes, and filtering to obtain the pulp or squeezed residue extract; in some embodiments, the feed - to - liquid ratio is selected to be 1:5; in some embodiments, the cellulase is added in an amount of 0.5%; in some embodiments, the temperature of the water bath is 45°C; in some embodiments, the extraction time is selected to be 60 minutes; in some embodiments, the ultrasonic power is 500W; in some embodiments, the ultrasonic treatment time is 30 minutes; Step 3: Concentrate the solution of the pulp extract of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart, or a combination of fruit juice and pomace extract, by rotary evaporation at 30-60 °C to obtain the ultrasonic aqueous extract of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart; in some embodiments, the rotary evaporation temperature is selected to be 50 °C; Step 4: Mix the ultrasonic aqueous extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or first mix the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1 and then extract. This includes the steps of.

[0044] In some embodiments, the extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart of the present invention are obtained by ultrasonic alcohol extraction. In some embodiments, Step 1: Remove the seeds or cores of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart respectively, take the pulp directly to obtain the pulp, or filter through 100-300 mesh gauze to obtain the fruit juice of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart, thereby separating the fruit juice from the pomace; in some embodiments, the gauze has a mesh number of 200 mesh; Step 2: Mix the pulp or squeezed residue of Mars needs veronica dick and Prunus serasifera Ehrhart with 20 - 100% ethanol at a feed - to - liquid ratio of 1:(2 - 10), add 0.2% - 1% (based on the weight of the squeezed residue or pulp) of cellulase, extract in a water bath at 30 - 50 °C for 30 - 100 minutes, treat with ultrasonic waves of 100 - 500 W for 20 - 60 minutes, and filter to obtain the pulp or squeezed residue extract; In some embodiments, the feed - to - liquid ratio is selected to be 1:5; In some embodiments, the concentration of ethanol used is 40%; In some embodiments, cellulase is added in an amount of 0.5%; In some embodiments, the temperature of the water bath is 45 °C; In some embodiments, the extraction time is selected to be 60 minutes; In some embodiments, the ultrasonic power is 500 W; In some embodiments, the ultrasonic treatment time is 30 minutes; Step 3: Concentrate the combined solution of the pulp extract of Mars needs veronica dick and Prunus serasifera Ehrhart, or the combination of fruit juice and squeezed residue extract by rotary evaporation at 30 - 60 °C to obtain the ultrasonic alcohol extract of Mars needs veronica dick and Prunus serasifera Ehrhart; In some embodiments, the rotary evaporation temperature is selected to be 50 °C; Optionally, the extract is evaporated until dry to remove the residual ethanol; Step 4: Mix the ultrasonic alcohol extracts of Mars needs veronica dick and Prunus serasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or first mix the raw materials of Mars needs veronica dick and Prunus serasifera Ehrhart in the aforementioned different ratios in Step 1 and then extract including the steps of

[0045] Ultra - high - pressure extraction In some embodiments, the extracts of Mars Neesvecana Deek and Prunus cerasifera Ehrhart of the present invention are obtained by ultra-high pressure extraction (for example, ultra-high pressure water extraction or ultra-high pressure alcohol extraction). As used herein, "ultra-high pressure" refers to a pressure exceeding 100 MPa (equivalent to 1000 atmospheres).

[0046] In some embodiments, the ultra-high pressure extraction is Step 1: Remove the seeds or cores of Mars Neesvecana Deek and Prunus cerasifera Ehrhart respectively, and take the pulp directly to obtain the pulp, or separate the juice from the squeezed residue; in some embodiments, the juice and the squeezed residue are separated by filtration using gauze (for example, 100 - 300 mesh gauze, for example, 200 mesh gauze); Step 2: Mix the pulp or the squeezed residue with an extraction medium (for example, water or ethanol), treat it under ultra-high pressure conditions (for example, 200 - 600 MPa, for example, 400 MPa) (for example, for 3 - 10 minutes, for example, 5 minutes), and filter to obtain the pulp or the squeezed residue extract; in some embodiments, the pulp or the squeezed residue is mixed with the extraction medium at a weight ratio of 1:(2 - 10) (for example, 1:5); in some embodiments, the ethanol is 50% - 95% ethanol; Step 3: Concentrate the combined solution of the pulp extract of Mars Neesvecana Deek and Prunus cerasifera Ehrhart or the combination of the juice and the squeezed residue extract to obtain the ultra-high pressure extract of Mars Neesvecana Deek and Prunus cerasifera Ehrhart; in some embodiments, the concentration is carried out by rotary evaporation; in some embodiments, the rotary evaporation temperature is 30 - 60 °C, for example, 50 °C; Step 4: Mixing the ultra-high pressure extracts of Mars Needs Vegetables and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, and 10:1) to prepare a composition, or first, mixing the raw materials of Mars Needs Vegetables and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, and then extracting including the step of.

[0047] In some embodiments, the extracts of Mars Needs Vegetables and Prunus cerasifera Ehrhart of the present invention are obtained by ultra-high pressure water extraction. In some embodiments, Step 1: Removing the seeds or cores of Mars Needs Vegetables and Prunus cerasifera Ehrhart respectively, and taking the pulp directly to obtain the pulp, or filtering through a 100-300 mesh gauze to obtain the fruit juices of Mars Needs Vegetables and Prunus cerasifera Ehrhart, thereby separating the juice from the squeezed residue; In some embodiments, the gauze has a mesh number of 200 meshes; Step 2: Mixing the pulp or squeezed residue of Mars Needs Vegetables and Prunus cerasifera Ehrhart with water at a feed-to-liquid ratio of 1:(2-10), treating under an ultra-high pressure of 200-600 MPa for 3-10 minutes, and filtering to obtain the pulp or squeezed residue extract; In some embodiments, the feed-to-liquid ratio is selected to be 1:5; In some embodiments, the ultra-high pressure is 400 MPa; In some embodiments, the ultra-high pressure treatment time is selected to be 5 minutes; Step 3: Concentrate the solution of the pulp extract of Vaccinium macrocarpon and Prunus cerasifera Ehrhart, or a combination of fruit juice and squeezed residue extract, by rotary evaporation at 30-60 °C to obtain the ultra-high pressure water extract of Vaccinium macrocarpon and Prunus cerasifera Ehrhart; in some embodiments, the rotary evaporation temperature is selected to be 50 °C; Step 4: Mix the ultra-high pressure water extracts of Vaccinium macrocarpon and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or first mix the raw materials of Vaccinium macrocarpon and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, and then extract. It includes the steps of.

[0048] In some embodiments, the extracts of Vaccinium macrocarpon and Prunus cerasifera Ehrhart of the present invention are obtained by ultra-high pressure alcohol extraction. In some embodiments, Step 1: Remove the seeds or cores of Vaccinium macrocarpon and Prunus cerasifera Ehrhart respectively, take the pulp directly to obtain the pulp, or filter through 100-300 mesh gauze to obtain the fruit juice of Vaccinium macrocarpon and Prunus cerasifera Ehrhart, thereby separating the fruit juice from the squeezed residue; in some embodiments, the gauze has a mesh number of 200 mesh; Step 2: Mix the pulp or squeezed residue of Mars needs vegetable caner Dick and Prunus cerasifera Ehrhart with ethanol at a feed-to-liquid ratio of 1:(2 - 10) at 20% - 100% (e.g., 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 100%), treat it under an ultra-high pressure of 200 - 600 MPa for 3 - 10 minutes, and filter to obtain a pulp or squeezed residue extract; in some embodiments, the feed-to-liquid ratio is selected to be 1:5; in some embodiments, the ultra-high pressure is 400 MPa; in some embodiments, the ultra-high pressure treatment time is selected to be 5 minutes; in some embodiments, the concentration of ethanol used is 40%; Step 3: Concentrate the combined solution of the pulp extract of Mars needs vegetable caner Dick and Prunus cerasifera Ehrhart, or a combination of fruit juice and squeezed residue extract, by rotary evaporation at 30 - 60 °C to obtain an ultra-high pressure alcohol extract of Mars needs vegetable caner Dick and Prunus cerasifera Ehrhart; in some embodiments, the rotary evaporation temperature is selected to be 50 °C; optionally, the extract is evaporated until dry to remove residual ethanol; Step 4: Mix the ultra-high pressure alcohol extracts of Mars needs vegetable caner Dick and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare a composition, or first mix the raw materials of Mars needs vegetable caner Dick and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1 and then extract including the steps of.

[0049] Subcritical extraction In some embodiments, the extracts of Mars needs vegetable caner Dick and Prunus cerasifera Ehrhart are obtained by subcritical extraction (e.g., subcritical water extraction or subcritical alcohol extraction).

[0050] In some embodiments, subcritical extraction comprises: Step 1: removing the seeds or cores of Vaccinium myrtillus and Prunus cerasifera Ehrhart respectively, and taking the pulp directly to obtain the pulp, or separating the juice from the pomace; in some embodiments, the juice and the pomace are separated by filtration using a gauze (e.g., 100-300 mesh gauze, e.g., 200 mesh gauze); Step 2: mixing the pulp or pomace with an extraction medium (e.g., water or ethanol), performing a subcritical extraction treatment (e.g., for 10-50 minutes, e.g., 30 minutes), and filtering to obtain a pulp or pomace extract; in some embodiments, the pulp or pomace is mixed with the extraction medium at a weight ratio of 1:(2-10) (e.g., 1:5); in some embodiments, the ethanol is 50%-95% ethanol; in some embodiments, the subcritical extraction treatment temperature is 100-150°C (e.g., 110°C); Step 3: concentrating the combined solution of the pulp extract or the juice and pomace extract to obtain a subcritical extract of Vaccinium myrtillus and Prunus cerasifera Ehrhart; in some embodiments, the concentration is performed by rotary evaporation; in some embodiments, the rotary evaporation temperature is 30-60°C, e.g., 50°C; Step 4: mixing the subcritical extracts of Vaccinium myrtillus and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1 and 10:1) to prepare a composition, or first mixing the raw materials of Vaccinium myrtillus and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1 and then extracting including the steps of.

[0051] In some embodiments, the extracts of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart of the present invention are obtained by subcritical water extraction. In some embodiments, Step 1: Remove the seeds or cores of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart respectively, and take the pulp directly to obtain the pulp, or filter through a 100-300 mesh gauze to obtain the fruit juices of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart, thereby separating the juice from the squeezed residue; in some embodiments, the gauze has a mesh number of 200 meshes; Step 2: Mix the pulp or squeezed residue of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart with water at a feed-to-liquid ratio of 1:(2-10), perform subcritical extraction at a treatment temperature of 100-150 °C for 10-50 minutes, and filter to obtain the pulp or squeezed residue extract; in some embodiments, the feed-to-liquid ratio is selected to be 1:5; in some embodiments, the treatment temperature is 110 °C; in some embodiments, the treatment time is 30 minutes; Step 3: Concentrate the combined solution of the pulp extract of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart, or the combination of fruit juice and squeezed residue extract, by rotary evaporation at 30-60 °C to obtain the subcritical water extract of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart; in some embodiments, the rotary evaporation temperature is selected to be 50 °C; Step 4: Mix the subcritical water extracts of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or first mix the raw materials of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart in the aforementioned different ratios in Step 1, and then extract. includes the steps of

[0052] In some embodiments, the extracts of Mars neesvecana Dick and Prunus serasifera Ehrhart of the present invention are obtained by subcritical alcohol extraction. In some embodiments, Step 1: Remove the seeds or cores of Mars neesvecana Dick and Prunus serasifera Ehrhart respectively, and take the pulp directly to obtain the pulp, or filter through a 100-300 mesh gauze to obtain the fruit juices of Mars neesvecana Dick and Prunus serasifera Ehrhart, thereby separating the juice from the squeezed residue; in some embodiments, the gauze has a mesh count of 200 meshes; Step 2: Mix the pulp or squeezed residue of Mars neesvecana Dick and Prunus serasifera Ehrhart with 20%-100% (e.g., 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 100%) ethanol at a feed-to-liquid ratio of 1:(2-10), perform subcritical extraction at a treatment temperature of 100-150°C for 10-50 minutes, and filter to obtain the pulp or squeezed residue extract; in some embodiments, the feed-to-liquid ratio is selected to be 1:5; in some embodiments, the treatment temperature is 110°C; in some embodiments, the treatment time is 30 minutes; Step 3: Concentrate the combined solution of the Mars neesvecana Dick and Prunus serasifera Ehrhart pulp extract, or the fruit juice and squeezed residue extract, by rotary evaporation at 30-60°C to obtain the subcritical alcohol extract of Mars neesvecana Dick and Prunus serasifera Ehrhart; in some embodiments, the rotary evaporation temperature is selected to be 50°C; optionally, the extract is evaporated until dry to remove the residual ethanol; Step 4: Mixing the subcritical alcohol extracts of Mars Needs Vegetables Dick and Prunus serasifera Ehrhart at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare a composition, or first, mixing the raw materials of Mars Needs Vegetables Dick and Prunus serasifera Ehrhart at the aforementioned different ratios in Step 1, and then extracting. including the step of

[0053] Macroporous resin separation In some embodiments, the extracts of Mars Needs Vegetables Dick and Prunus serasifera Ehrhart are obtained by macroporous resin separation.

[0054] In some embodiments, the macroporous resin separation is Step 1: Obtaining the extracts of Mars Needs Vegetables Dick and Mars Needs Vegetables Dick by the aforementioned different extraction methods (leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, etc.); or obtaining the pulp of Mars Needs Vegetables Dick and Mars Needs Vegetables Dick by directly taking the pulp. Step 2: Mixing the extracts or pulp of Mars Needs Vegetable Canner and Mars Needs Vegetable Canner with 95 - 100% ethanol at a ratio of 1:(2 - 10) (by weight) (e.g., 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, or 1:10) for 1 - 5 hours (e.g., 3 hours), then filtering to obtain alcohol - precipitated juice and precipitate, and subjecting the alcohol - precipitated juice to rotary evaporation at 30 - 60°C (e.g., 30°C, 40°C, 50°C, or 60°C) to remove ethanol, thereby obtaining clear alcohol - precipitated juice; In some embodiments, the extracts or pulp of Mars Needs Vegetable Canner and Mars Needs Vegetable Canner are also subjected to enzymatic degradation purification treatment by adding pectinase to obtain clear enzymatically - decomposed juice, where the enzymatic degradation temperature is 20 - 60°C (e.g., 20°C, 30°C, 40°C, 50°C, or 60°C), the enzymatic degradation time is 30 - 240 minutes (e.g., 30 minutes, 60 minutes, 90 minutes, 120 minutes, 150 minutes, 180 minutes, 210 minutes, or 240 minutes), and the amount of added pectinase is 100 - 1000 ppm (e.g., 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm, 800 ppm, 900 ppm, or 1000 ppm); Step 3: Adsorbing the clear alcohol - precipitated juice or clear enzymatically - decomposed juice with macroporous adsorption resin, and then performing gradient elution with water and 20% - 100% ethanol (e.g., some selected from 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol, and 100% ethanol) while recovering the eluate of each gradient; In some embodiments, the elution rate is 1 - 5 BV / h (1 - 5 column volumes); Step 4: Subjecting the eluate to rotary evaporation at 30 - 60°C (e.g., 30°C, 40°C, 50°C, or 60°C) to remove ethanol, thereby obtaining different macroporous resin - separated samples including the steps of

[0055] Mars Needsvechjana Deek and Prunus serasifera Ehrhart may be separately extracted to obtain extracts and then mixed at a specific ratio to obtain a composition, or the raw materials of Mars Needsvechjana Deek and Prunus serasifera Ehrhart may first be mixed in different ratios and then extracted to obtain a composition. As described above, the product forms of the composition of the present invention can be fresh fruits, fruit juices, jams, powders (e.g., lyophilized powders), candies (e.g., tablet candies), oral liquids, capsules, tablets, dried fruits, preserved fruits, wines, or pastries. Among them, fruit juices, jams, powders (e.g., lyophilized powders), candies (e.g., tablet candies), oral liquids, capsules, and tablets can be prepared from extracts obtained by leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation, or other extraction methods. Furthermore, the product forms of the composition of the present invention can be drugs, ordinary foods, functional foods, or health foods. In some embodiments, in product forms including fruit juices, jams, powders (e.g., lyophilized powders), candies (e.g., tablet candies), oral liquids, capsules, tablets, dried fruits, preserved fruits, wines, or pastries, the composition of the present invention can be used as the main raw material accounting for, for example, 30 wt% or more, 40 wt% or more, 50 wt% or more, 60 wt% or more, 70 wt% or more, 80 wt% or more, 90 wt% or more, or 100 wt% of all raw materials; optionally, the product may also contain other components, such as water, sweetening substances, or other types of fruits or their extracts.

[0056] Exemplary methods for products of Mars Needsvechjana Deek and Prunus serasifera Ehrhart (e.g., fresh fruits, fruit juices, jams, powders, tablet candies, oral liquids, capsules) are provided below.

[0057] Fresh fruits In some embodiments, the fresh fruits of Mars Needsvechjana Deek and Prunus serasifera Ehrhart are Step 1: A step of collecting Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart from one month before ripening until full ripening, preferably a step of collecting fruits on a sunny day; Step 2: A step of picking to remove rotten or discolored fruits; Step 3: A step of storing in refrigeration after picking; Step 4: Combining fresh fruits of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) The method can be obtained by including the steps.

[0058] The obtained fresh fruits of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart can be eaten directly.

[0059] Fruit juice In some embodiments, the fruit juice of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart Step 1: A step of removing the seeds or cores of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart respectively, and then taking the pulp; Step 2: Filtering the pulp (for example, using 100 - 300 mesh gauze) to obtain the turbid fruit juice of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart; in some embodiments, the gauze has a mesh number of 200 mesh; Step 3: Mixing the cloudy juices of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare a composition, or first, mixing the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, then taking the pulp and preparing the juice It can be obtained by a method including the steps of

[0060] In some embodiments, the juices of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart are Step 1: Removing the seeds or cores of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart respectively, and then taking the pulp; Step 2: Filtering the pulp (for example, using 100 - 300 mesh gauze), adding pectinase for enzymatic decomposition and filtering to obtain the clear juices of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart; in some embodiments, the gauze has a mesh count of 200 mesh; Step 3: Mixing the clear juices of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare a composition, or first, mixing the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, then taking the pulp and preparing the juice It can also be obtained by a method including the steps of

[0061] In some embodiments, the juice of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart can also be prepared using the extracts obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation, or other extraction methods as raw materials.

[0062] Jam In some embodiments, the jam of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart Step 1: Remove the seeds or cores of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart respectively, and then take the pulp; Step 2: Boil the pulp in water and concentrate it under heating to obtain the jam of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart; Step 3: Mix the jams of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, and 10:1) to prepare the composition, or first mix the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, then take the pulp and prepare the jam It can be obtained by a method including the steps.

[0063] In some embodiments, the jam of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart can also be prepared using the extracts or juice obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation, or other extraction methods as raw materials.

[0064] Powder In some embodiments, the powder of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart Step 1: Remove the seeds or cores of Mars needs vervet canna deck and Prunus serasifera Ehrhart respectively, and then take the pulp; Step 2: Freeze-dry the pulp to obtain a freeze-dried powder of the pulp; Step 3: Mix the freeze-dried powders of Mars needs vervet canna deck and Prunus serasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, and 10:1) to prepare a composition, or first, mix the raw materials of Mars needs vervet canna deck and Prunus serasifera Ehrhart in the aforementioned different ratios in Step 1, then take the pulp, and prepare a freeze-dried powder It can be obtained by a method including the steps.

[0065] In some embodiments, the powders of Mars needs vervet canna deck and Prunus serasifera Ehrhart are Step 1: Remove the seeds or cores of Mars needs vervet canna deck and Prunus serasifera Ehrhart respectively, and then take the pulp; Step 2: Filter the pulp (for example, using 100 - 300 mesh gauze) to obtain the fruit juices of Mars needs vervet canna deck and Prunus serasifera Ehrhart; In some embodiments, the gauze has a mesh number of 200 mesh; Step 3: Freeze-dry the fruit juices to obtain a freeze-dried powder of the fruit juices; Step 4: Mixing the freeze-dried powders of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, and 10:1) to prepare the composition, or first, mixing the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart at the aforementioned different ratios in Step 1, then taking the pulp and preparing the freeze-dried powder can also be obtained by a method including the steps of.

[0066] In some embodiments, the powders of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart can also be obtained using a belt dryer with reference to the above steps.

[0067] In some embodiments, the powders of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart can also be prepared using, as raw materials, the extracts obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation, or other extraction methods by freeze-drying or belt drying.

[0068] Tablet candies In some embodiments, the tablet candies of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart are Step 1: Removing the seeds or cores of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart respectively, and then taking the pulp; Step 2: Freeze-drying or belt-drying the pulp to obtain a powder; Step 3: Tableting the powder and the adjuvant to obtain tablet candies; Step 4: Mixing tablets of Mars needsvetch canna and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare a composition, or first, mixing the raw materials of Mars needsvetch canna and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, then taking the pulp and preparing tablets It can be obtained by a method including the steps of

[0069] In some embodiments, the tablets of Mars needsvetch canna and Prunus cerasifera Ehrhart Step 1: Removing the seeds or cores of Mars needsvetch canna and Prunus cerasifera Ehrhart respectively, and then taking the pulp; Step 2: Filtering the pulp (for example, using 100 - 300 mesh gauze) to obtain the fruit juices of Mars needsvetch canna and Prunus cerasifera Ehrhart; In some embodiments, the gauze has a mesh count of 200 mesh; Step 3: Freeze - drying or belt - drying the fruit juices to obtain a powder; Step 4: Tableting the powder and adjuvant to obtain tablets; Step 5: Mixing tablets of Mars needsvetch canna and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare a composition, or first, mixing the raw materials of Mars needsvetch canna and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, then taking the pulp and preparing tablets It can also be obtained by a method including the step of

[0070] In some embodiments, the tablet candies of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart can also be prepared using the extracts obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods as raw materials.

[0071] Oral liquid In some embodiments, the oral liquid of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart Step 1: Prepare extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) (for example, by the above steps, steps of taking the pulp or making it into fruit juice, preparing them from fresh fruits by leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation); Step 2: Sterilize the extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart to obtain the oral liquid of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart It can be obtained by a method including the step of

[0072] In some embodiments, the oral liquid of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart Step 1: Prepare extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) (for example, by the above steps, steps of taking the pulp or making it into fruit juice, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, preparing them from fresh fruits by macroporous resin separation); Step 2: Add pectinase for enzymatic hydrolysis and filter to obtain clear fruit juice; Step 3: Sterilize the clear fruit juice of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart to obtain an oral liquid of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart can also be obtained by a method including the steps.

[0073] Capsule In some embodiments, the capsules of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart are Step 1: Remove the seeds or cores of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart respectively, and then take the pulp; Step 2: Freeze-dry or belt-dry the pulp to obtain a freeze-dried powder of the pulp; Step 3: Mix the powders of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, and 10:1) to prepare a composition, or first, mix the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart at the aforementioned different ratios in Step 1, then take the pulp and prepare the powder; Step 4: Filling the powder into the capsule shell to obtain a capsule It can also be obtained by a method including the steps of

[0074] In some embodiments, the capsules of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart can be Step 1: Removing the seeds or cores of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart respectively, and then taking the pulp; Step 2: Filtering the pulp (for example, using 100 - 300 mesh gauze) to obtain the fruit juices of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart; In some embodiments, the gauze has a mesh number of 200 mesh; Step 3: Freeze - drying or belt - drying the fruit juices to obtain a powder; Step 4: Mixing the powders of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, and 10:1) to prepare a composition, or first mixing the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in the aforementioned different ratios in Step 1, then taking the pulp and preparing a powder; Step 5: Filling the powder into the capsule shell to obtain a capsule It can also be obtained by a method including the steps of

[0075] In some embodiments, the capsules of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart can also be prepared by using the extracts obtained by the aforementioned leaching extraction, ultrasonic extraction, ultra - high pressure extraction, sub - critical extraction, macroporous resin separation or other extraction methods as raw materials.

[0076] Beneficial effects In the present invention, natural Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart are utilized. The prepared compositions of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart have good effects in terms of resistance to atherosclerosis. The combined use of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart has better effects than either of the two used alone, achieving a synergistic effect. Furthermore, the further use of the composition in combination with a drug has better effects than those of the composition or the drug used alone, achieving a synergistic effect.

Example

[0077] Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention.

[0078] (Example 1) The Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart products described in this example refer to the fresh fruits of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart.

[0079] Malus niedzwetzkyana Dieck (Hongxun 1) and Prunus cerasifera Ehrhart are preferably harvested on sunny days from one month before maturity until full maturity, but are picked to remove rotten or discolored fruits, and then the fruits are stored refrigerated. The obtained fresh fruits of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart may be combined in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) for direct consumption.

[0080] (Example 2) The Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart products described in this example refer to the cloudy fruit juices of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart.

[0081] The collected Mars Needs Vegetable Canner Dick (Hongxun 1) and Prunus Cerasifera Ehrhart, after removing the seeds or cores, had their pulp taken, and the pulp was filtered through a 200-mesh gauze to obtain the cloudy fruit juices of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart. The cloudy fruit juices of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart were mixed in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or the raw materials of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart were first mixed in the aforementioned different ratios, then the pulp was taken to obtain the fruit juice.

[0082] (Example 3) The Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart products described in this example refer to the clear fruit juices of Mars Needs Vegetable Canner Dick and Prunus Cerasifera Ehrhart.

[0083] The recovered Malus niedzwetzkyana Dieck (Hongxun 1) and Prunus cerasifera Ehrhart were de-seeded or cored, then the pulp was taken, and the pulp was filtered through a 200-mesh gauze to obtain the turbid juice of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart. Pectinase was added for enzymatic hydrolysis and then filtered to obtain clear juice. The clear juices of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were mixed in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were first mixed in the aforementioned different ratios and then the pulp was taken to obtain the juice.

[0084] (Example 4) The Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart products described in this example refer to the jams of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart.

[0085] Malus niedzwetzkyana Dieck (Hongxun 1) and Prunus cerasifera Ehrhart were de-seeded or cored, then the pulp was taken and boiled in water under heating to obtain a relatively thick jam. The jams of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were mixed in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were first mixed in the aforementioned different ratios and then the pulp was taken to obtain the jam.

[0086] (Example 5) The Mars Neesvecana Dick and Prunus Cerasifera Ehrhart products described in this example refer to the powders of Mars Neesvecana Dick and Prunus Cerasifera Ehrhart.

[0087] After removing the seeds or cores, the pulp of Mars Neesvecana Dick (Hongxun 1) and Prunus Cerasifera Ehrhart was taken and freeze-dried to obtain the freeze-dried powder of the pulp. The freeze-dried powders of Mars Neesvecana Dick and Prunus Cerasifera Ehrhart were mixed in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or the raw materials of Mars Neesvecana Dick and Prunus Cerasifera Ehrhart were first mixed in the aforementioned different ratios, then the pulp was taken to obtain the powder.

[0088] (Example 6) The Mars Neesvecana Dick and Prunus Cerasifera Ehrhart products described in this example refer to the tablet candies of Mars Neesvecana Dick and Prunus Cerasifera Ehrhart.

[0089] Malus niedzwetzkyana Dieck (Hongxun 1) and Prunus cerasifera Ehrhart were processed by removing the seeds or cores, taking the pulp, and freeze-drying it to obtain a freeze-dried powder of the pulp. The freeze-dried powder of the pulp and an adjuvant were made into tablets to obtain tablet candies. The tablet candies of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were mixed at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare a composition, or the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were first mixed at the aforementioned different ratios, then the pulp was taken to obtain tablet candies.

[0090] (Example 7) The products of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart described in this example refer to the water extraction oral liquid of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart.

[0091] (1) Malus niedzwetzkyana Dieck (Hongxun 1) and Prunus cerasifera Ehrhart were processed by removing the seeds or cores, taking the pulp, and obtaining the pulp of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart. (2) The pulp of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart was mixed with water at a feed-to-liquid ratio of 1:5, cellulase was added, water extraction was carried out at 50 °C for 60 minutes, and then filtered to obtain a pulp extract. (3) The extract was concentrated by rotary evaporation at 50 °C to obtain the water extract of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart. (4) The extracts of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart were mixed in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition. (5) The composition of the extracts of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart was sterilized to obtain an oral liquid.

[0092] (Example 8) The Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart products described in this example refer to the alcohol leaching extract oral liquid of Mars Needs Vegetables Dick (Hongxun 1) and Prunus Serasifera Ehrhart.

[0093] The treatment was carried out with reference to steps (1) to (5) of Example 7, and the pulp was extracted with 20%, 40%, 60% or 100% ethanol at a feed-to-liquid ratio of 1:5, respectively. The alcohol extract was evaporated until dry to remove the residual ethanol.

[0094] (Example 9) The Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart products described in this example refer to the water leaching extract oral liquid of Mars Needs Vegetables Dick (Hongxun 3) and Prunus Serasifera Ehrhart. The preparation steps were the same as those in Example 7.

[0095] (Example 10) The Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart products described in this example refer to the water leaching extract oral liquid of Mars Needs Vegetables Dick (Hongxun 6) and Prunus Serasifera Ehrhart. The preparation steps were the same as those in Example 7.

[0096] (Example 11) The products of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart described in this example refer to the ultrasonic water extract oral liquids of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart.

[0097] (1) After removing the seeds or cores of Mars Needs Vegetable Canner Dick (Hongxun 1) and Prunus cerasifera Ehrhart, the pulp was taken to obtain the pulp of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart. (2) The pulp of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart was mixed with water at a feed-to-liquid ratio of 1:5, 0.5% (based on the weight of the pulp) of cellulase was added, the pulp was extracted in a water bath at 45 °C for 60 minutes, the treatment was carried out with ultrasonic waves of 500 W for 30 minutes, and then filtered to obtain the pulp extract. (3) The extract was concentrated by rotary evaporation at 50 °C to obtain the ultrasonic water extract of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart. (4) The extracts of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart obtained by extraction were mixed at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition. (5) The composition of the extracts of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart was sterilized to obtain the oral liquid.

[0098] (Example 12) The products of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart described in this example refer to the ultrasonic water extract oral liquids of Mars Needs Vegetable Canner Dick (Hongxun 6) and Prunus cerasifera Ehrhart. The preparation steps were the same as those in Example 10.

[0099] (Example 13) The Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart products described in this example refer to the ultrasonic alcohol extract oral liquids of Mars Needs Vegetable Canner Dick (Hongxun 1) and Prunus cerasifera Ehrhart.

[0100] The treatment was carried out with reference to steps (1) to (5) of Example 10, and the pulp was extracted with 20%, 40%, 60% or 100% ethanol at a feed-to-liquid ratio of 1:5 respectively. The alcohol extract was evaporated until dry to remove the residual ethanol.

[0101] (Example 14) The Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart products described in this example refer to the ultra-high pressure water extract oral liquids of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart.

[0102] (1) After removing the seeds or cores of Mars Needs Vegetable Canner Dick (Hongxun 1) and Prunus cerasifera Ehrhart, the pulp was taken to obtain the pulp of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart. (2) The pulp of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart was mixed with water at a feed-to-liquid ratio of 1:5, treated under ultra-high pressure of 400 MPa for 5 minutes, and then filtered to obtain a pulp extract. (3) The extract was concentrated by rotary evaporation at 50 °C to obtain the ultra-high pressure water extract of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart. (4) The extracts of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart were mixed at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare a composition. (5) The composition of the extracts of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart was sterilized to obtain an oral liquid.

[0103] (Example 15) The Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart products described in this example refer to the ultra-high pressure water extraction oral liquid of Mars Needs Vegetables Dick (Hongxun 6) and Prunus Serasifera Ehrhart. The preparation steps were the same as those in Example 13.

[0104] (Example 16) The Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart products described in this example refer to the ultra-high pressure alcohol extraction oral liquid of Mars Needs Vegetables Dick (Hongxun 1) and Prunus Serasifera Ehrhart.

[0105] The treatment was carried out with reference to steps (1)-(5) of Example 13, and the pulp was extracted with 50% ethanol at a feed-to-liquid ratio of 1:5. The alcohol extract was evaporated until dry to remove the residual ethanol.

[0106] (Example 17) The Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart products described in this example refer to the subcritical water extraction oral liquid of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart.

[0107] (1) After removing the seeds or cores of Mars Needs Vegetables Dick (Hongxun 1) and Prunus Serasifera Ehrhart, the pulp was taken to obtain the pulp of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart. (2) The pulp of Mars Needs Vegetables Dick and Prunus Serasifera Ehrhart was mixed with water at a feed-to-liquid ratio of 1:5 and subjected to subcritical extraction at 110°C for 30 minutes, and then filtered to obtain a pulp extract. (3) The extract was concentrated by rotary evaporation at 50 °C to obtain the subcritical water extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart. (4) The extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were mixed at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition. (5) The composition of the extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart was sterilized to obtain the oral liquid.

[0108] (Example 18) The Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart products described in this example refer to the jams of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart.

[0109] The water leaching extract oral liquid of Malus niedzwetzkyana Dieck (Hongxun 1) and Prunus cerasifera Ehrhart was first prepared with reference to Example 7, and then boiled in water under heating to obtain a relatively thick jam with or without the addition of adjuvants. The jams of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were mixed at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the composition, or the raw materials of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were first mixed at the aforementioned different ratios and then prepared into jam.

[0110] (Example 19) The Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart products described in this example refer to the powders of Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart.

[0111] The water leaching extract oral liquid of Mars Needs Vegetable Canner Dick (Hongxun 1) and Prunus Serasifera Ehrhart was first prepared with reference to Example 7, and then freeze-dried to obtain a freeze-dried powder with or without the addition of adjuvants. The freeze-dried powders of Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart were mixed in different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare a composition, or the raw materials of Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart were first mixed in the aforementioned different ratios and then prepared into a powder.

[0112] (Example 20) The Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart products described in this example refer to the jams of Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart.

[0113] The ultrasonic extraction oral liquids of Mars Needs Vegetable Canner Dick (Hongxun 1) and Prunus cerasifera Ehrhart were first prepared with reference to Example 11, and then boiled in water under heating to obtain relatively thick jams with or without the addition of adjuvants. The jams of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart were mixed at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the compositions, or the raw materials of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart were first mixed at the aforementioned different ratios and then prepared into jams.

[0114] (Example 21) The products of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart described in this example refer to the powders of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart.

[0115] The ultrasonic extraction oral liquids of Mars Needs Vegetable Canner Dick (Hongxun 1) and Prunus cerasifera Ehrhart were first prepared with reference to Example 11, and then belt-dried to obtain powders with or without the addition of adjuvants. The powders of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart were mixed at different ratios (by weight) (1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1) to prepare the compositions, or the raw materials of Mars Needs Vegetable Canner Dick and Prunus cerasifera Ehrhart were first mixed at the aforementioned different ratios and then prepared into powders.

[0116] (Example 22) The products of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart described in this example refer to the transparent alcohol-precipitated juice of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart.

[0117] The water extract concentrate of Malus niedzwetzkyana Dieck (Hongxun 1) and Prunus cerasifera Ehrhart was first prepared with reference to Example 7. The concentrate was mixed with 95% ethanol at a weight ratio of 1:2 for 3 hours, then filtered to obtain a transparent alcohol-precipitated juice, and the ethanol was removed by rotary evaporation to obtain a composition of the transparent alcohol-precipitated juice of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart.

[0118] (Example 23) The products of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart described in this example refer to the macroporous resin separation products of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart.

[0119] The transparent alcohol-precipitated juice of Malus niedzwetzkyana Dieck (Hongxun 1) and Prunus cerasifera Ehrhart was first prepared with reference to Example 22. The transparent alcohol-precipitated juice was adsorbed by macroporous adsorption resin, and gradient elution was carried out in turn with water and 20%, 40%, 60%, and 100% ethanol at an elution rate of 3 BV / h (3 column volumes) while collecting the eluents of each gradient. The eluate was subjected to rotary evaporation at 30 - 60 °C to obtain different macroporous resin separation samples.

[0120] Evaluation of the effects of the products of the above examples on resistance to atherosclerosis: Experimental Example 1: Evaluation of the effects of compositions of different ratios of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart for preventing atherosclerosis in mice (1) Test method Wild-type C57 mice were used as a normal control group, and rosuvastatin was used as a positive drug. The effectiveness of the composition sample in preventing atherosclerosis was investigated in an atherosclerotic model of ApoE- / - mice fed a high-fat diet. The atherosclerotic model (i.e., the model group) was established 12 weeks after modeling, and the control group (i.e., the normal group) was fed normal food for 12 weeks. The sample administration group was administered the test sample simultaneously with modeling, the normal group and the model group were administered an equal dose of distilled water, and the formation of plaques in the aortic atherosclerosis of mice was observed after 12 weeks of continuous administration. The plaque area was measured after dissection, and the plaque percentage was calculated.

[0121] (2) Test results The test results are shown in Table 1. In the atherosclerosis prevention model, Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart were extracted by water extraction according to Example 7, and a water extraction extract of Malus niedzwetzkyana Dieck and a water extraction extract of Prunus cerasifera Ehrhart were obtained. The extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart had certain effects when used alone at a dose of 40 g / kg compared with the model group, and had more significant effects when used in combination at a ratio in the range of 1:10 to 10:1 (the dose of the composition was 40 g / kg). The composition could significantly reduce the formation of plaques in the arterial wall compared with the model group and was effective in preventing atherosclerosis.

[0122] The q value was calculated by the formula of Jin reported in the literature: q = E(A + B) / (EA + EB - EA·EB) [where E(A + B) is the inhibition rate of the two drugs used in combination, EA and EB are the respective inhibition rates of the drugs used alone, q < 1 indicates that the two drugs used in combination have an antagonistic effect, and q ≥ 1 indicates that the two drugs used in combination have a synergistic effect.] as calculated by

[0123] As can be seen from Table 1, the combined use of Mars Needs Verifier Dick and Prunus serasifera Ehrhart had one or more q values, that is, its effect was significantly better than that of the two used alone. The composition had an additive effect in preventing atherosclerosis.

Table 1

[0124] Experimental Example 2: Evaluation of the effects of compositions of different ratios of Mars Needs Verifier Dick and Prunus serasifera Ehrhart for treating atherosclerosis in mice (1) Test method Wild-type C57 mice were used as the normal control group, and rosuvastatin was used as the positive drug. The effectiveness (i.e., treatment effect) of the composition sample in the resistance to atherosclerosis was examined in an atherosclerosis model of ApoE- / - mice with a high-fat diet introduced. The atherosclerosis model was established 17 weeks after modeling, and the control group was given normal food for 17 weeks. The sample administration group was continuously administered the test sample for 4 weeks 17 weeks after modeling. Then, the formation of plaques of aortic atherosclerosis in mice was observed, the plaque area was measured after dissection, and the plaque percentage was calculated.

[0125] (2) Test results The test results are shown in Table 2. In the atherosclerotic treatment model, Mars Needs Vegetable Cana Dick and Prunus Serratifera Heart were extracted by water leaching according to Example 7, and a water leaching extract of Mars Needs Vegetable Cana Dick and a water leaching extract of Prunus Serratifera Heart were obtained. The extract of Mars Needs Vegetable Cana Dick and the extract of Prunus Serratifera Heart had certain effects when used alone at a dose of 40 g / kg, compared with the model group, and had more significant effects when used in combination at a ratio in the range of 1:10 to 10:1 (the dose of the composition was 40 g / kg). The composition can significantly reduce the plaque area in the arterial wall and is effective in the treatment of atherosclerosis.

[0126] The q value was calculated with reference to Jin's formula in Experimental Example 1.

[0127] As can be seen from Table 2, Mars Needs Vegetable Cana Dick and Prunus Serratifera Heart used in combination had one or more q values, that is, their effects were significantly better than those of the two used alone. The composition had a synergistic effect in the treatment of atherosclerosis.

Table 2

[0128] Experimental Example 3: Evaluation of the effects of compositions of different doses of Mars Needs Vegetable Cana Dick and Prunus Serratifera Heart for preventing atherosclerosis in mice (1) Test method Wild-type C57 mice were used as a normal control group, and rosuvastatin was used as a positive drug. The effectiveness of the composition sample in preventing atherosclerosis was examined in an atherosclerotic model of ApoE- / - mice with a high-fat diet introduced. The atherosclerotic model (i.e., the model group) was established 12 weeks after modeling, and the control group (i.e., the normal group) was given normal food for 12 weeks. The sample administration group was administered the test sample simultaneously with modeling, the normal group and the model group were administered an equal dose of distilled water, the formation of plaques of aortic atherosclerosis in mice was observed after 12 weeks of continuous administration, the plaque area was measured after dissection, and the plaque percentage was calculated.

[0129] (2) Test results The test results are shown in Table 3. In the atherosclerosis prevention model, the effects of compositions of different doses of Mars Needs Vegetables Dick and Prunus serasifera Ehrhart were compared. Mars Needs Vegetables Dick and Prunus serasifera Ehrhart were extracted by water leaching according to Example 7 to obtain a water leaching extract of Mars Needs Vegetables Dick and a water leaching extract of Prunus serasifera Ehrhart. The extract of Mars Needs Vegetables Dick and the extract of Prunus serasifera Ehrhart had weak effects when used alone at doses of 20 g / kg and 10 g / kg respectively compared with the model group, and had more significant effects when used in combination (the doses of the composition were 20 g / kg and 10 g / kg respectively). The composition can significantly reduce the formation of plaques in the arterial wall and is effective in preventing atherosclerosis.

[0130] The q value was calculated with reference to Jin's formula in Experimental Example 1.

[0131] As can be seen from Table 3, the combined use of Mars Needs Vegetable Canner Dick and Prunus Serratifera Ehrhart had one or more q values, that is, its effect was superior to that of the two used alone. The composition had a synergistic effect. It was found that compositions of different doses of Mars Needs Vegetable Canner Dick and Prunus Serratifera Ehrhart could achieve a synergistic effect in preventing atherosclerosis in mice.

Table 3

[0132] Experimental Example 4: Evaluation of the effects of compositions of different doses of Mars Needs Vegetable Canner Dick and Prunus Serratifera Ehrhart for treating atherosclerosis in mice (1) Test method Wild-type C57 mice were used as a normal control group, and rosuvastatin was used as a positive drug. The effectiveness (i.e., treatment effect) of the composition sample in resistance to atherosclerosis was examined in an atherosclerosis model of ApoE− / − mice with a high-fat diet introduced. The atherosclerosis model was established 17 weeks after modeling, and the control group was given normal food for 17 weeks. The sample administration group was continuously administered the test sample for 4 weeks 17 weeks after modeling. Thereafter, the formation of plaques of aortic atherosclerosis in mice was observed, the plaque area was measured after dissection, and the plaque percentage was calculated.

[0133] (2) Test results The test results are shown in Table 4. In the atherosclerosis treatment model, the effects of compositions of different doses of Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart were compared. Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart were extracted by water extraction according to Example 7, and a water extraction extract of Mars Needs Vegetable Canner Dick and a water extraction extract of Prunus Serasifera Ehrhart were obtained. The extract of Mars Needs Vegetable Canner Dick and the extract of Prunus Serasifera Ehrhart had weak effects when used alone at doses of 20 g / kg and 10 g / kg, respectively, compared with the model group, and had more significant effects when used in combination (the doses of the compositions were 20 g / kg and 10 g / kg, respectively). The composition was able to significantly reduce the plaque area in the arterial wall.

[0134] The q value was calculated with reference to Jin's formula in Experimental Example 1.

[0135] As can be seen from Table 4, Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart used in combination had one or more q values, that is, their effects were superior to those of the two used alone. The composition had a synergistic effect. It was found that compositions of different doses of Mars Needs Vegetable Canner Dick and Prunus Serasifera Ehrhart could achieve a synergistic effect in the treatment of atherosclerosis in mice.

Table 4

[0136] Experimental Example 5: Evaluation of the Effect of a Composition Combined with a Statin Drug for Treating Atherosclerosis in Mice (1) Test Method Wild-type C57 mice were used as a normal control group, rosuvastatin and atorvastatin were used as positive drugs, and the effectiveness of the composition mixture sample in resistance to atherosclerosis (i.e., treatment effect) was examined in an atherosclerosis model of ApoE- / - mice with a high-fat diet introduced. The atherosclerosis model was established 17 weeks after modeling, and the control group was given normal food for 17 weeks. The sample administration group was continuously administered the test sample for 4 weeks 17 weeks after modeling. Thereafter, plaque formation of aortic atherosclerosis in mice was observed, the plaque area was measured after dissection, and the plaque percentage was calculated.

[0137] (2) Test results The test results are shown in Table 5. The composition was obtained by extraction according to Example 7. In the atherosclerosis treatment model, the composition of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in the dose range of 40 g / kg to 10 g / kg, combined with high / low doses of positive drugs (rosuvastatin or atorvastatin), could significantly reduce the plaque area in the arterial wall compared with the model group and was effective in treating atherosclerosis. The combined use of the composition and the drug had a synergistic effect compared with their individual use (the calculation was performed with reference to Experimental Example 1).

Table 5

[0138] Experimental Example 6: Evaluation of the effect of a composition combined with other drugs for treating atherosclerosis in mice (1) Test method Wild-type C57 mice were used as a normal control group, aspirin, clopidogrel, colchicine, and probucol were used as positive drugs, and the effectiveness of the composition mixture sample in the resistance to atherosclerosis (i.e., treatment effect) was examined in an atherosclerosis model of ApoE− / − mice fed a high-fat diet. The atherosclerosis model was established 17 weeks after modeling, and the control group was given normal food for 17 weeks. The sample administration group was continuously administered the test sample for 4 weeks 17 weeks after modeling, and then the formation of plaques of aortic atherosclerosis in the mice was observed. The plaque area was measured after dissection, and the plaque percentage was calculated.

[0139] (2) Test results The test results are shown in Table 6. The effects of different combinations of positive drugs and compositions on the resistance to atherosclerosis were compared. The composition was obtained by extraction according to Example 7. In the atherosclerosis treatment model, compositions of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in a dose range of 40 g / kg to 10 g / kg, combined with positive drugs (aspirin, clopidogrel, colchicine, and probucol), could significantly reduce the plaque area in the arterial wall compared with the model group and were effective in the treatment of atherosclerosis. The combined use of the composition and the drug had a synergistic effect compared with their individual use (the calculation was performed with reference to Experimental Example 1). [Table 6]

[0140] Experimental Example 7: Difference in the effects of different varieties of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart in an animal model (1) Test method Wild-type C57 mice were used as a normal control group, and rosuvastatin was used as a positive drug. The effectiveness of the sample in resistance to atherosclerosis (i.e., treatment effect) was examined in an atherosclerotic model of ApoE− / − mice with a high-fat diet introduced. The atherosclerotic model was established 17 weeks after modeling, and the control group was given normal food for 17 weeks. The sample administration group was continuously administered the test sample for 4 weeks 17 weeks after modeling. Subsequently, the formation of plaques of aortic atherosclerosis in the mice was observed, the plaque area was measured after dissection, and the plaque percentage was calculated.

[0141] (2) Test results As can be seen from Table 7, the inhibition rates of Mars Nees Vicanner Dick Hongxun 1 and Prunus cerasifera Ehrhart Hongxun 3 were similar, and there was no significant difference. It was found that there was no significant difference in the effects of different varieties of Mars Nees Vicanner Dick in the animal model. Similarly, there was no significant difference in the effects of different varieties of Prunus cerasifera Ehrhart in the animal model.

Table 7

[0142] Experimental Example 8: Difference in the effects of compositions of Mars Nees Vicanner Dick and Prunus cerasifera Ehrhart obtained by different extraction methods in an animal model (1) Test method Wild-type C57 mice were used as a normal control group, and rosuvastatin was used as a positive drug. The effectiveness of the composition sample in the resistance to atherosclerosis (i.e., the treatment effect) was examined in an atherosclerotic model of ApoE- / - mice fed a high-fat diet. The atherosclerotic model was established 17 weeks after modeling, and the control group was fed normal food for 17 weeks. The sample administration group was continuously administered the test sample for 4 weeks 17 weeks after modeling. Subsequently, the formation of plaques in the aortic atherosclerosis of the mice was observed, the plaque area was measured after dissection, and the plaque percentage was calculated.

[0143] (2) Test results As can be seen from Table 8, the compositions obtained by Examples 7 and 8 through extraction with ethanol at different concentrations had good effects in the treatment of atherosclerosis. The compositions of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart obtained by different extraction methods had good effects in the animal model and were found to be superior to the two used alone.

Table 8

[0144] Experimental Example 9: Difference in the effects of the compositions of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart obtained by different extraction methods in an animal model (1) Test method Wild-type C57 mice were used as a normal control group, and rosuvastatin was used as a positive drug. The effectiveness of the composition sample in the resistance to atherosclerosis (i.e., the treatment effect) was examined in an atherosclerotic model of ApoE- / - mice with a high-fat diet introduced. The atherosclerotic model was established 17 weeks after modeling, and the control group was given normal food for 17 weeks. The sample administration group was continuously administered the test sample for 4 weeks 17 weeks after modeling. Subsequently, the formation of plaques of aortic atherosclerosis in the mice was observed, and the mice were dissected to calculate the plaque area (percentage).

[0145] (2) Test results As can be seen from Table 9, the compositions obtained by Examples 22 and 23 by gradient elution of the macroporous adsorption resin with ethanol at different concentrations had good effects in the treatment of atherosclerosis. The compositions of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart obtained by different extraction methods had good effects in the animal model and were found to be superior to the two used alone.

Table 9

[0146] The above examples are merely illustrative of the technical solutions of the present invention and not restrictive. Modifications and equivalent substitutions to these are intended to be included within the scope of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A first component which is an extract of Malus niedzwetzkyana Dieck or Malus niedzwetzkyana Dieck; and A second component which is an extract of Prunus cerasifera Ehrhart or Prunus cerasifera Ehrhart A composition comprising the same.

2. The weight ratio of the first component to the second component is from 1:10 to 10:1 (for example, 1:9 to 10:1, 1:8 to 10:1, 1:7 to 10:1, 1:6 to 10:1, 1:5 to 10:1, 1:4 to 10:1, 1:3 to 10:1, 1:2 to 10:1, 1:1 to 10:1, 1:10 to 9:1, 1:9 to 9:1, 1:8 to 9:1, 1:7 to 9:1, 1:6 to 9:1, 1:5 to 9:1, 1:4 to 9:1, 1:3 to 9:1, 1:2 to 9:1, 1:1 to 9:1, 1:10 to 8:1, 1:9 to 8:1, 1:8 to 8:1, 1:7 to 8:1, 1:6 to 8:1, 1:5 to 8:1, 1:4 to 8:1, 1:3 to 8:1, 1:2 to 8:1, 1:1 to 8:1, 1:10 to 7:1, 1:9 to 7:1, 1:8 to 7:1, 1:7 to 7:1, 1:6 to 7:1, 1:5 to 7:1, 1:4 to 7:1, 1:3 to 7:1, 1:2 to 7:1, 1:1 to 7:1, 1:10 to 6:1, 1:9 to 6:1, 1:8 to 6:1, 1:7 to 6:1, 1:6 to 6:1, 1:5 to 6:1, 1:4 to 6:1, 1:3 to 6:1, 1:2 to 6:1, 1:1 to 6:1, 1:10 to 5:1, 1:9 to 5:1, 1:8 to 5:1, 1:7 to 5:1, 1:6 to 5:1, 1:5 to 5:1, 1:4 to 5:1, 1:3 to 5:1, 1:2 to 5:1, 1:1 to 5:1, 1:10 to 4:1, 1:9 to 4:1, 1:8 to 4:1, 1:7 to 4:1, 1:6 to 4:1, 1:5 to 4:1, 1:4 to 4:1, 1:3 to 4:1, 1:2 to 4:1, 1:1 to 4:1, 1:10 to 3:1, 1:9 to 3:1, 1:8 to 3:1, 1:7 to 3:1, 1:6 to 3:1, 1:5 to 3:1, 1:4 to 3:1, 1:3 to 3:1, 1:2 to 3:1, 1:1 to 3:1, 1:10 to 2:1, 1:9 to 2:1, 1:8 to 2:1, 1:7 to 2:1, 1:6 to 2:1, 1:5 to 2:1, 1:4 to 2:1, 1:3 to 2:1, 1:2 to 2:1, 1:1 to 2:1, 1:10 to 1:1, 1:9 to 1:1, 1:8 to 1:1, 1:7 to 1:1, 1:6 to 1:1, 1:5 to 1:1, 1:4 to 1:1, 1:3 to 1:1, 1:2 to 1:1, 1:10 to 5:1, 1:10 to 2:1, 1:10 to 1:1, 1:5 to 10:1, 1:5 to 5:1, 1:5 to 2:1, 1:5 to 1:1, 1:2 to 10:1, 1:2 to 5:1, 1:2 to 2:1, 1:2 to 1:1, 1:1 to 10:1, 1:1 to 5:1, 1:1 to 2:1, for example, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1,The composition according to claim 1, which is 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1).

3. Whether the extract of Malus niedzwetzkyana Dieck or Malus niedzwetzkyana Dieck is obtained from / by fresh fruits, pulp extraction or juice extraction of fresh fruits, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods; Or, whether the extract of Prunus cerasifera Ehrhart or Prunus cerasifera Ehrhart is obtained from / by fresh fruits, pulp extraction or juice extract of fresh fruits, leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods, the composition according to Claim 1.

4. The composition is in the form of fresh fruits, fruit juices, jams, powders, candies, oral liquids, capsules, tablets, dried fruits, preserved fruits, wines, or pastries; Optionally, forms including fruit juices, jams, powders, candies, oral liquids, capsules, tablets, etc. can be prepared using extracts of Malus niedzwetzkyana Dieck and Prunus cerasifera Ehrhart obtained by leaching extraction, ultrasonic extraction, ultra-high pressure extraction, subcritical extraction, macroporous resin separation or other extraction methods as raw materials; Or the composition is in the form of a medicine (for example, traditional Chinese medicine, Chinese patented medicine, natural medicine, etc.), ordinary food or functional food or health food, the composition according to Claim 1.

5. Malus niedzwetzkyana Dieck refers to the fruit of a plant of the genus Malus Mill of Rosaceae that is red and fresh. Preferably, Malus niedzwetzkyana Dieck is selected from Hongxun 1 (Hong Xun 1), Hongxun 3 (Hong Xun 3), Hongxun 4 (Hong Xun 4), Hongxun 6 (Hong Xun 6), Hongxun 8 (Hong Xun 8), Red Love, etc.; Or, Prunus cerasifera Ehrhart is selected from the red fruit of Prunus cerasifera Ehrhart, the black fruit of Prunus cerasifera Ehrhart, the purple fruit of Prunus cerasifera Ehrhart, the dark purple fruit of Prunus cerasifera Ehrhart, the yellow fruit of Prunus cerasifera Ehrhart, etc., and the composition according to claim 1.

6. A first product, which is the composition according to claim 1; A second product, which is a medicament for preventing and / or treating atherosclerotic diseases A combination product comprising the above.

7. Is the atherosclerotic disease selected from atherosclerotic diseases of the aorta and its main branches, coronary atherosclerotic diseases, cerebral atherosclerotic diseases, renal atherosclerotic diseases, mesenteric atherosclerotic diseases, atherosclerotic diseases of the extremities, etc.; Or, the medicament for preventing and / or treating atherosclerotic diseases is selected from statins (such as rosuvastatin, atorvastatin, simvastatin), ezetimibe, PCSK9 inhibitors, aspirin, clopidogrel, colchicine, connamab, probucol, idebenone, etc., and Chinese patented pharmaceutical preparations, and the combination product according to claim 6.

8. The combined product according to claim 6, wherein the weight ratio of the first product to the second product is from 1000:0.001 to 1000:33.

4.

9. Use of the composition according to any one of claims 1 to 5 in the preparation of a medicament (such as traditional Chinese medicine, Chinese patented medicine, natural medicine, etc.), a normal food, a functional food or a health food for preventing and / or treating atherosclerotic diseases.

10. Use of the combined product according to any one of claims 6 to 8 in the preparation of a medicament (such as traditional Chinese medicine, Chinese patented medicine, natural medicine, etc.) for preventing and / or treating atherosclerotic diseases.

11. The use according to claim 9, wherein the atherosclerotic disease is selected from atherosclerotic diseases of the aorta and its main branches, coronary atherosclerotic diseases, cerebral atherosclerotic diseases, renal atherosclerotic diseases, mesenteric atherosclerotic diseases, atherosclerotic diseases of the extremities, etc.

12. The use according to claim 10, wherein the atherosclerotic disease is selected from atherosclerotic diseases of the aorta and its main branches, coronary atherosclerotic diseases, cerebral atherosclerotic diseases, renal atherosclerotic diseases, mesenteric atherosclerotic diseases, atherosclerotic diseases of the extremities, etc.