Licorice carbon coating material for reducing hepatotoxicity of drugs, its manufacturing method and uses

Abstract

Disclosed are a licorice carbon coating material for reducing the hepatotoxicity of drugs, a method for producing the same, and uses thereof. The coating material is licorice carbon, and the method for producing licorice carbon is obtained by pulverizing licorice slices, sieving, stir-frying over high heat for 1-2 hours, spraying clear water, sealing and cooling. The yield of licorice carbon prepared by this method is about 45%, which is about 10% higher than the highest yield reported in the existing reports. The obtained licorice carbon is a black or grayish-black powder, with a slight fragrance, a light and slightly sweet taste, less than 3% impurities, and less than 10% moisture. The licorice carbon prepared by this method can effectively reduce the hepatotoxicity of drugs, be used as a coating for drug-induced hepatotoxic drugs, reduce liver damage after patients take medicine, and has the potential for wide application.

Description

Technical Field

[0001] (Cross - reference to Related Applications) This application claims the benefit of Chinese Application No. 2022106390547, filed on June 7, 2022. The entire content of the application number 2022106390547 is hereby incorporated by reference in its entirety.

[0002] (Technical Field) The present invention belongs to the field of medicine, and particularly relates to a glycyrrhiza carbon coating material for reducing the hepatotoxicity of drugs, its manufacturing method and uses.

Background Art

[0003] Traditional Chinese carbonized medicines are one of the drugs with very distinctive preparation characteristics. There are two types of traditional carbon manufacturing methods: stir-frying to carbonize and calcining to carbonize. That is, the product to be prepared is put into a pot and stir-fried until the surface turns charred black and the inside turns charred brown, or heated to a specified degree. When manufacturing carbon, it is necessary to "preserve the nature", prevent ash formation, and avoid reignition. Traditional Chinese carbonized medicines have effects such as hemostasis, antidiarrhea, hypoglycemic, analgesic, liver protection, anti-inflammatory, and anti-ulcer in clinical practice. Currently, the number of types of carbonized medicines used clinically has reached more than 70. Licorice carbon is a processed product of the dried roots and rhizomes of the leguminous plant Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. Its essence is a part of licorice that has been carbonized and is widely used in the surface coating of water pills in traditional Chinese medicine preparations. Zhuanggu Guanjie Pills and Kanggu Zengsheng Pills included in the Pharmacopoeia of the People's Republic of China (2020 Edition) are manufactured with licorice carbon coating. The manufactured water pills have uniform color, luster, and are not easily deteriorated. At the same time, licorice has a harmonizing effect on other medicines and does not affect the effect of the preparation even when used in the coating. In recent research, the nano-components separated and purified from carbonized medicines were identified and analyzed by combining characterization techniques such as transmission electron microscopy and Fourier transform infrared spectroscopy with pharmacological activity experiments. The results showed that the carbon nano-components are the material basis for the medicinal effects of the corresponding carbonized medicinal materials. Nano-components have also been discovered in licorice carbon, and it has been proven that the nano-components have an anti-ulcer effect using a mouse acute alcoholic gastric ulcer model (Reference: For example, Zhu Yafan, Research on the Original Appearance of the "Roasting" Method in Zhongjing and the Anti-ulcer Effect of Roasted Licorice [D]. Beijing University of Chinese Medicine, 2019.). Further pharmacological activities of licorice carbon still need to be explored deeply.

[0004] With the extensive use of Chinese herbal medicines both at home and abroad, in recent years, safety issues / events of Chinese medicines, represented by liver damage, have been frequently reported. In particular, among Chinese medicines that have traditionally been regarded as non-toxic, there are many reports of liver damage, and among them, Polygonum multiflorum is relatively prominent. China's drug regulatory authorities have issued liver damage warnings and regulatory notices for preparations containing Polygonum multiflorum many times, including Yangxue Shengfa Capsules, Shouwu Yanshou Tablets, Shouwu Yanshou Granules, etc. With the wider application of traditional Chinese medicine and the deepening of people's understanding of traditional Chinese medicine, new safety issues of Chinese medicines may be discovered one after another. On the basis of ensuring clinical efficacy, it is possible to effectively solve the safety problems of Chinese medicines by reducing the hepatotoxicity of Chinese medicines, seeking benefits and avoiding damages, and minimizing the risks of clinical use. At present, the main methods for reducing the hepatotoxicity of Chinese medicines are detoxification by preparation and detoxification by formulation. For example, the traditional preparation method of Polygonum multiflorum, "nine steaming and nine sunning", and the modern mainstream steaming or steaming with black soybean juice can both reduce the hepatotoxicity of Polygonum multiflorum. Clinically, Polygonum multiflorum is mainly detoxified by being formulated with Poria cocos, Glycyrrhiza uralensis, etc. In a study investigating the detoxification effect of the combination of Polygonum multiflorum with Poria cocos, Glycyrrhiza uralensis, and Panax notoginseng, it was shown that Poria cocos, Glycyrrhiza uralensis, and Panax notoginseng all had significant detoxification effects and were dose-dependent. Among them, the detoxification effect of Poria cocos was the most significant. However, the formulation of the prescription flavors and the preparation of medicinal materials of Chinese patent medicines are fixed, and changing them will affect the clinical efficacy.

[0005] Therefore, there is a lack of technology that can effectively reduce the hepatotoxicity of drugs, is applicable to most Chinese patent medicines, and does not affect the medicinal efficacy.

Summary of the Invention

[0006] In order to solve the above technical problems, the present invention provides a Glycyrrhiza carbonata coating material for reducing the hepatotoxicity of drugs, its manufacturing method, and uses.

[0007] Specifically, the present invention is realized by the following technical means.

[0008] In a first aspect, the present invention provides a manufacturing method of a Glycyrrhiza carbonata coating material for reducing the hepatotoxicity of drugs, which includes the following steps.

[0009] Crush the licorice slices, sieve them through a 1.5 - 2.0 cm sieve, set the starting temperature of the stir - frying machine at 120°C - 160°C, set the heating temperature at 250 - 350°C, set the heating time at 1.0 - 2.0 hours, stir - fry until the surface is charred black and the inside is charred yellow, end the heating when the smoke becomes thick, spray a small amount of clear water to extinguish the sparks, after the stir - frying machine cools down to room temperature, take out the stir - fried licorice, dry it, crush it, and sieve it through a No. 6 sieve to obtain the licorice charcoal coating material.

[0010] As an alternative, in the above - mentioned manufacturing method, the licorice slices are licorice slices that meet the quality standard requirements of the "Pharmacopoeia of the People's Republic of China".

[0011] As an alternative, in the above - mentioned manufacturing method, the licorice slices are prepared and processed products of the dried roots and rhizomes of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of the Leguminosae family.

[0012] As an alternative, the above - mentioned manufacturing method includes the following steps.

[0013] Crush the licorice slices, sieve them through a 1.6 cm sieve, set the starting temperature of the stir - frying machine at 150°C, set the heating temperature at 300°C, set the heating time at 1.5 hours, stir - fry until the surface is charred black and the inside is charred yellow, end the heating when the smoke becomes thick, spray a small amount of clear water to extinguish the sparks, wait until the stir - frying machine cools down to room temperature, then take out the stir - fried licorice, dry it, crush it, and sieve it through a No. 6 sieve to obtain the licorice charcoal coating material.

[0014] On the second aspect, the present invention provides a licorice charcoal coating material for reducing the hepatotoxicity of a drug obtained by using the manufacturing method described in the first aspect above. This licorice charcoal coating material is a black or gray - black powder, has a slight fragrance, a light taste and is slightly sweet, with less than 3% impurities and less than 10% moisture by weight percentage.

[0015] In a third aspect, the present invention provides the use of the licorice carbon coating material described in the second aspect above for the surface coating of water pills of traditional Chinese medicine preparations. The water pills coated with the licorice carbon coating material have a uniform color, luster, and are not easily deteriorated.

[0016] In a fourth aspect, the present invention provides the use of the licorice carbon coating material described in the second aspect above for the production of a coating material that reduces the hepatotoxicity of drugs.

[0017] As an alternative, in the above use, the drug is a drug having drug-induced hepatotoxicity.

[0018] As an alternative, in the above use, the drug having drug-induced hepatotoxicity is Polygonum multiflorum, Tripterygium wilfordii, Aristolochia fangchi or Psoralea corylifolia.

[0019] The present invention has the following beneficial effects over the prior art.

[0020] (1) The production method of the present invention improves the yield of licorice carbon by about 10%. The currently reported yield of the production method of licorice carbon is at most 35%, and the yield of the production process is low, leaving room for improvement (see: CN201810717845.0). The yield of licorice carbon produced by the method of the present invention is about 45%, which is about 10% higher than the yield of the conventional report.

[0021] (2) There is little pharmacological activity research on licorice carbon. As a very characteristic drug in the preparation of traditional Chinese medicine carbon drugs, its pharmacological activity has great potential for further exploration. The present invention discovers for the first time that the licorice carbon produced by the method of the present invention has the effect of reducing the hepatotoxicity of drugs. Furthermore, by using this as a coating for drugs having drug-induced hepatotoxicity, liver damage after patients take medicine can be reduced, and it has a broad application prospect.

Embodiments for Carrying Out the Invention

[0022] The present invention will be further described based on specific examples below. It should be understood that the specific examples described here are only used for the description of the present invention and do not limit the scope of the present invention.

[0023] When specific technologies and conditions are not described in the examples, the technologies and conditions described in the literature in this field or the instructions of the products shall be followed. When the manufacturers of the reagents and equipment used are not described, they are general products that can be purchased through regular channels.

[0024] Unless otherwise specified, all the experimental methods in the following examples are common methods. Unless otherwise specified, the test materials used in the following examples are commercially available products.

[0025] Manufacturing Example: The manufacturing process of the licorice charcoal coating material for reducing the hepatotoxicity of the drug of the present invention is as follows. That is, licorice slices are pulverized, sieved through a 1.6 cm sieve, the starting temperature of the stir-frying machine is set at 150 °C, the heating temperature is set at 300 °C, the heating time is set at 1.5 hours. When the surface becomes charred black and the inside becomes charred yellow and the smoke becomes thick, the heating is terminated, a small amount of clear water is sprayed to extinguish the sparks, and after waiting for the stir-frying machine to cool to room temperature, the stir-fried licorice is taken out, dried, pulverized, and sieved through a No. 6 sieve to obtain the licorice charcoal coating material.

[0026] The production of licorice charcoal was carried out according to the production method of the present invention, and the production situation is summarized below.

Table 1

[0027] The above data indicate that the preparation technology of licorice charcoal is mature and can meet the production demand.

[0028] The produced licorice charcoal should meet the following requirements. Particle size: All should be sieved through a No. 5 sieve, and those sieved through a No. 6 sieve should be 98% or more. The impurity content shall not exceed 3% (General Principles 2301 of Chinese Pharmacopoeia, 2020 Edition). Inspection method: 1. Take an appropriate amount of the test sample, spread it out, and observe it with the naked eye or a magnifying glass (5 - 10 times magnification) to remove impurities. If there are separable impurities, use an appropriate sieve to separate the impurities. 2. Weigh each type of impurity separately and calculate the content (%) in the test sample. The moisture content shall not exceed 10.0% (Method 2 of General Principles 0832 of Chinese Pharmacopoeia, 2020 Edition). Hot air drying method: Take 25 g of the test sample. If the diameter or length of the test sample exceeds 3 mm, quickly process it into particles or fragments with a diameter or length not exceeding 3 mm before weighing, and spread it evenly in a flat weighing bottle that has been dried to a constant weight. Make sure the thickness does not exceed 5 mm, and for loose test samples, make sure it does not exceed 10 mm, then weigh precisely. Open the lid of the bottle and dry it at 100 - 105 °C for 5 hours, close the lid of the bottle, transfer it to a desiccator, cool it for 30 minutes, and weigh precisely. Then, dry it at the above temperature for 1 hour, cool it, and weigh it, ensuring that the difference between two consecutive weighings does not exceed 5 mg. Calculate the moisture content (%) in the test sample based on the reduced weight.

[0029] Effect examples: The licorice carbon used in the following effect experiments is the licorice carbon produced in the "Manufacturing Example" part of the present invention.

[0030] Effect Example 1: Investigation of the effect of the combination of Polygonum multiflorum Thunb. and licorice carbon on human normal hepatocyte line L02 cells 1. Cell origin and culture conditions: Cell origin: Human normal hepatocyte line L02 cells were purchased from Shanghai Meixuan Biotechnology Co., Ltd. Culture conditions: L02 cells were routinely cultured in RPMI - 1640 culture medium containing 10% fetal bovine serum, in a cell culture incubator at 37 °C with 5% CO 2 at saturated humidity, and the culture medium was changed once every 1 - 2 days. Logarithmic growth phase L02 cells with good condition and vigorous growth were used for subsequent experiments. Origin of medicinal materials: Polygonum multiflorum Thunb. medicinal materials were purchased from Kangmei Pharmaceutical Co., Ltd., extracted and concentrated with 70% ethanol, and a medicinal liquid with the required crude drug concentration for the experiment was prepared for standby.

[0031] 2. Experimental grouping: Blank control group (i.e., normal saline group), Polygonum multiflorum group (dose: 20 mg·mL -1 ), Polygonum multiflorum and high-dose carbonized licorice combination group (Polygonum multiflorum dose: 20 mg·mL -1 , carbonized licorice dose: 15 mg·mL -1 ), Polygonum multiflorum and medium-dose carbonized licorice combination group (Polygonum multiflorum dose: 20 mg·mL -1 , carbonized licorice dose: 10 mg·mL -1 ), Polygonum multiflorum and low-dose carbonized licorice combination group (Polygonum multiflorum dose: 20 mg·mL -1 , carbonized licorice dose: 5 mg·mL -1 ).

[0032] 3. Sample preparation: Crush the Polygonum multiflorum medicinal materials and sieve them with a 1.5 cm sieve for reserve. Take the Polygonum multiflorum and the mixed powders of Polygonum multiflorum and high- and low-concentration carbonized licorice respectively, perform ultrasonic extraction (output: 200 W, frequency: 40 kHz) twice with 10 times the amount of 70% ethanol for 1 hour each time. Combine the two extraction solutions, recover ethanol under reduced pressure, dry and concentrate in a 50°C water bath to obtain an extract. Dissolve it with DMSO, make up the volume to 25 mL in a volumetric flask, store it in a 4°C refrigerator, prepare it to the highest required concentration with a complete culture medium before use, and prepare other concentrations by serial dilution with a complete culture medium, and sterilize it with a 0.22 μm microporous filter to obtain it.

[0033] 4. Measurement of the toxicity of each experimental group to L02 cells by CCK-8 method: Take L02 cells in the logarithmic growth phase, resuspend them after digestion, adjust the cell density to 4×10 4 cells / mL, inoculate 200 μL of cells into each well of a 96-well plate. After 24 hours, change the liquid, and add 200 μL of the prepared Polygonum multiflorum and the mixed solutions of Polygonum multiflorum and high- and low-concentration carbonized licorice to each well. Set up a negative control group (add an equal volume of complete culture medium) in the experiment, and set up 5 replicate wells for each group. After adding the drug for 48 hours, discard the supernatant of each well, gently wash it once with PBS, add 100 μL of 5% CCK-8 reagent to each well, incubate in a 37°C incubator for 1 hour, and measure the absorbance (A) at a wavelength of 450 nm with an enzyme-labeling instrument.

[0034] Calculate the inhibition rate (IR) of L02 cells using the following formula: IR = (1 - A 投薬群平均 / A 陰性対照群平均 ) × 100%.

[0035] 5. Measurement of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities in the culture supernatant:

[0036] After cell seeding and grouping, add the drug to each group and culture for 48 hours, then collect the cell supernatant and measure the activities of ALT, AST, and LDH in the supernatant according to the instructions of the reagent kit.

[0037] 6. Data analysis: Perform statistical analysis using SPSS 17.0 software. The measured data between groups are

Number

[0038] 7. Experimental results: The results are shown in Table 2. Compared with the blank control group, the polygonum multiflorum group and the combination group of polygonum multiflorum and carbonized licorice significantly inhibited the growth of L02 cells (P < 0.01). The combinations of different doses of carbonized licorice and polygonum multiflorum still showed different degrees of inhibitory effects on L02 cells, but the combinations of medium and high doses of carbonized licorice and polygonum multiflorum showed a significantly lower inhibitory effect than the polygonum multiflorum group (P < 0.05 or P < 0.01). Also, there is a certain dose-response relationship in the inhibitory effect, indicating that carbonized licorice significantly reduces the inhibitory effect of polygonum multiflorum on the proliferation of L02 cells.

Table 2

[0039] The results are shown in Table 3. Compared with the blank control group, the polygonum multiflorum group significantly increased the activities of ALT, AST, and LDH in the L02 cell culture supernatant (P<0.01). Compared with the administration of polygonum multiflorum alone, the combined administration of high, medium, and low doses of carbonized licorice root and polygonum multiflorum significantly reduced the levels of ALT, AST, and LDH increased by polygonum multiflorum (P<0.05 or P<0.01). In addition, there is a clear dose-response relationship in the reduction of the levels of ALT, AST, and LDH.

Table 3

[0040] Effect Example 2: Consideration of the influence of the combination of polygonum multiflorum and carbonized licorice root on the liver of mice In this effect example, the contents of ALT and AST in mouse serum are used as important indicators of liver poisoning. This is because when liver cells are damaged during liver poisoning, cell permeability increases, and AST and ALT in cell mitochondria and liver cells are released into the blood in large quantities, and their contents exceed the normal level, thereby reflecting the degree of liver damage. When damaged by liver poisoning, mainly oxidative stress reactions occur, and in this process, superoxide dismutase (SOD) and malondialdehyde (MDA) indirectly reflect the ability to remove free radicals and the degree of attack by free radicals respectively. In addition, due to organ damage caused by liver poisoning, the body automatically performs self-repair, cell tissue proliferates, the mass of the liver increases, and the liver body index becomes larger.

[0041] 1. Experimental method: Fifty SPF-class KM mice (half male and half female, body weight 30.0±2.0 g) were purchased from Hunan Slake Jingda Experimental Animal Co., Ltd., and the mice were randomly divided into 5 groups based on body weight. Blank control group (i.e., physiological saline group), polygonum multiflorum group (dose 20 g·kg -1 ), combined group of polygonum multiflorum and high-dose carbonized licorice root (polygonum multiflorum dose 20 g·kg -1 , carbonized licorice root dose 3 g·kg -1 ), combined group of polygonum multiflorum and medium-dose carbonized licorice root (polygonum multiflorum dose 20 g·kg -1 , carbonized licorice root dose 2 g·kg -1) The compatibility group of Polygonum multiflorum and low-dose carbonized licorice (Polygonum multiflorum dosage: 20 g·kg -1 , carbonized licorice dosage: 1 g·kg -1 ). There are 10 mice in each group, and they are raised separately by male and female.

[0042] Grind the medicinal materials of Polygonum multiflorum, sieve them with a 1.5 cm sieve, and keep them as a reserve. Take the powders mixed with carbonized licorice of different concentrations from Polygonum multiflorum respectively, and perform ultrasonic extraction twice with 10 times the amount of 70% ethanol (output 200 W, frequency 40 kHz, 1 hour each). Combine the two extraction liquids, recover ethanol under reduced pressure, concentrate in a water bath at 50 °C to obtain an extract, and keep it as a reserve. Add the required amount of water for the experiment to prepare a medicinal liquid with the corresponding crude drug concentration.

[0043] Intervene continuously for 15 days, administer orally once a day, and collect serum and liver samples 12 hours later without diet restriction on the 16th day. Use an enzyme-labeling device to detect the indexes of serum ALT, serum AST, liver tissue MDA, and liver tissue SOD. During the drug intervention period, measure the body weight of the mice once every two days, adjust the dosing volume, and closely observe the changes in the physiological signs of the mice during the dosing period.

[0044] Statistical method: All the measurement data in this experiment are

Number

[0045] 2. Experimental results: As a result of oral administration to mice for 15 days, there was no death phenomenon in the mice of the five groups, and there were no obvious differences in physiological signs such as body weight, diet status, hair color, and excretion.

[0046] Observing the data in Table 4, compared with the blank control group, the liver somatic indices (liver somatic index = liver mass / body mass × 100%) of the polygonum multiflorum group and the group with polygonum multiflorum combined with carbonized licorice root are both higher than that of the blank control group (P < 0.05), indicating that the administration of polygonum multiflorum and the combination of polygonum multiflorum and carbonized licorice root have liver toxicity and cause liver damage. However, the liver somatic indices of the combined administration of polygonum multiflorum and carbonized licorice root are all lower than those of the single administration of polygonum multiflorum, with significant differences (P < 0.01), and it is also shown that the liver somatic index depends on the administration concentration of carbonized licorice root. This indicates that carbonized licorice root can effectively reduce the liver toxicity caused by polygonum multiflorum.

[0047] Comparing the contents of AST and ALT in the sera of mice in each group, the contents of AST and ALT in the polygonum multiflorum group and the group with polygonum multiflorum combined with carbonized licorice root are higher than those of the blank control group (P < 0.01). The levels of AST and ALT in the combined administration of polygonum multiflorum and carbonized licorice root are all lower than those of the single administration group of polygonum multiflorum (P < 0.01 or P < 0.05), and it is also shown that the levels of AST and ALT depend on the administration concentration of carbonized licorice root. From the perspective of liver cell damage, it indicates that carbonized licorice root can effectively reduce the damage of liver cells caused by polygonum multiflorum.

Table 4

[0048] Observing the data in Table 5, the MDA contents of the single administration of polygonum multiflorum and the combined administration of polygonum multiflorum and carbonized licorice root are higher than those of the blank control group (P < 0.01 or P < 0.05). However, the MDA of the combined administration of polygonum multiflorum and carbonized licorice root is lower than that of the single administration group of polygonum multiflorum (P < 0.01 or P < 0.05); the activities of SOD in the single administration of polygonum multiflorum and the combined administration of polygonum multiflorum and carbonized licorice root are both lower than those of the blank control group (P < 0.01), but the activity of SOD in the combined administration of polygonum multiflorum and carbonized licorice root is higher than that of the single administration of polygonum multiflorum (P < 0.01 or P < 0.05). From the perspective of the microscopic liver oxidative stress reaction, it indicates that carbonized licorice root can effectively reduce the liver damage effect caused by polygonum multiflorum.

Table 5

[0049] Generally, Polygonum multiflorum has hepatotoxicity, and administration of a certain dose of Polygonum multiflorum can cause liver damage. However, the combination of Polygonum multiflorum and carbonized licorice can effectively reduce liver damage caused by Polygonum multiflorum. It has been shown that the combination of Polygonum multiflorum and carbonized licorice mainly inhibits the increase in liver body index, the increase in the contents of AST and ALT, and the increase in MDA content caused by Polygonum multiflorum, and increases the activity of SOD. Therefore, carbonized licorice can effectively reduce the hepatotoxicity of Polygonum multiflorum, and the combination of carbonized licorice and Polygonum multiflorum can improve the drug use safety of Polygonum multiflorum.

[0050] From this, it is obvious that the carbonized licorice produced by the method of the present invention can effectively reduce the hepatotoxicity of drugs, can be used as a coating for drugs with drug-induced hepatotoxicity, can reduce liver damage after patients take medicine, and has broad application prospects.

[0051] Obviously, those skilled in the art can make various modifications and deformations to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and deformations of the present invention are within the scope of the claims of the present invention and its equivalent technologies, the present invention is intended to include these modifications and deformations.

Claims

1. A method for manufacturing a glycyrrhiza carbon coating material for reducing the hepatotoxicity of drugs, characterized by comprising the following steps; Crush the sliced licorice root, sieve it through a sieve with a mesh size of 1.5 - 2.0 cm, set the starting temperature of the stir-frying machine to 120°C - 160°C, set the heating temperature to 250 - 350°C, and set the heating time to 1.0 - 2.0 hours. Stir-fry until the surface becomes charred black and the inner surface becomes charred yellow. When the smoke becomes thick, stop heating, spray a small amount of clear water to extinguish the sparks. After the stir-frying machine cools down to room temperature, take out the stir-fried licorice, dry it, crush it, and sieve it through a No. 6 sieve to obtain the glycyrrhiza carbon coating material.

2. The manufacturing method according to Claim 1, characterized in that the sliced licorice root conforms to the quality standard requirements of the "Pharmacopoeia of the People's Republic of China".

3. The manufacturing method according to Claim 1 or Claim 2, characterized in that the sliced licorice root is a processed product of the dried roots and rhizomes of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of the Leguminosae family.

4. The manufacturing method according to any one of Claims 1 to 3, characterized by comprising the following steps; Crush the sliced licorice root, sieve it through a sieve with a mesh size of 1.6 cm, set the starting temperature of the stir-frying machine to 150°C, set the heating temperature to 300°C, and set the heating time to 1.5 hours. Stir-fry until the surface becomes charred black and the inner surface becomes charred yellow. When the smoke becomes thick, stop heating, spray a small amount of clear water to extinguish the sparks. Wait until the stir-frying machine cools down to room temperature, then take out the stir-fried licorice, dry it, crush it, and sieve it through a No. 6 sieve to obtain the glycyrrhiza carbon coating material.

5. A glycyrrhiza carbon coating material for reducing the hepatotoxicity of drugs manufactured by the manufacturing method according to any one of Claims 1 to 4, characterized in that the glycyrrhiza carbon coating material is a black or grayish-black powder, has a slight fragrance, a light taste and a slightly sweet taste, with less than 3% impurities and less than 10% moisture by weight percentage.

6. The use of the glycyrrhiza carbon coating material according to Claim 5 as the surface coating of water pills in traditional Chinese medicine preparations, characterized in that the water pills coated with the glycyrrhiza carbon coating material have a uniform color, luster and are not easily deteriorated.

7. The use of the glycyrrhiza carbon coating material according to Claim 5 in the manufacture of a coating material for reducing the hepatotoxicity of drugs.

8. The use according to claim 7, wherein the drug is a drug having drug-induced hepatotoxicity.

9. The use according to claim 8, wherein the drug having drug-induced hepatotoxicity is Polygonum multiflorum Thunb., Tripterygium wilfordii Hook. f., Aristolochia fangchi Y. C. Wu ex L. D. Chow & S. M. Hwang or Psoralea corylifolia L.

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