Genome amplification module with branch space adjacent to extract inlet

Abstract

The present invention relates to a genome amplification module having a branch space adjacent to an inlet through which an extract flows, and provides a genome amplification module in which a branch space that is wider and deeper than the extract flow passage is formed adjacent to the inlet, and after the branch space is filled with extract, the extract can be simultaneously injected along each extract flow passage.

Description

[Technical Field]

[0001] The present invention relates to a genome amplification module having a branch space adjacent to an inlet through which an extract enters. [Background technology]

[0002] In recent years, advances in biotechnology have made it possible to understand the causes of diseases at the genetic level, which has led to an increasing demand for the manipulation and biochemical analysis of biological samples to cure or prevent human diseases.

[0003] In addition to disease diagnosis, there is also a demand for technology to extract and analyze nucleic acids from biological samples and samples containing cells in various fields, such as new drug development, preliminary testing for the presence or absence of viral or bacterial infections, and forensic medicine.

[0004] Meanwhile, Patent Document 5, an apparatus developed by the present applicant, discloses an extraction device that pretreats an input sample to prepare an extract containing a genome. The extract produced by the extraction device is transferred to an amplification module connected to the extraction device, and the extract input to a storage section of the amplification module is amplified through a nucleic acid amplification reaction. The storage section stores probes that specifically bind to target sequences and contain fluorescent substances, so if the genome of the extract contains the target sequence, fluorescence can be observed through the nucleic acid amplification reaction. Then, the presence or absence of fluorescence can be used to determine whether the individual from whom the sample was taken has a disease / viral infection.

[0005] Meanwhile, in the case of an amplification module having multiple storage compartments, primers / probes for detecting different targets may be stored in each storage compartment. If the extracts introduced into the storage compartments are mixed during the injection / amplification process, inaccurate detection results may be obtained. Furthermore, relatively uniform results can only be obtained if the same amount of extract is injected into each storage compartment, but with existing genome amplification modules, there has been a problem where more extract is injected into storage compartments that are less affected by gravity.

[0006] In response to this, the present inventors have focused on and completed the present invention in order to solve the problems of conventional genome amplification modules. [Prior art documents] [Patent documents]

[0007] [Patent Document 1] Korean Patent No. 10-2346703 [Patent Document 2] Korean Patent Registration No. 10-2416335 [Patent Document 3] Korean Patent Registration No. 10-2293717 [Patent Document 4] Korean Patent Registration No. 10-2375252 [Patent Document 5] Korean Patent Registration No. 10-2362853 Summary of the Invention [Problem to be solved by the invention]

[0008] According to the present invention, an inner chamber containing reagents necessary for genome extraction is provided separately from an outer chamber, and the upper and lower parts of the inner chamber are sealed, thereby solving the problem of reagents contained in a single chamber leaking out from conventional genome extraction devices.

[0009] Another object of the present invention is to provide a genome extraction device including a safety clip for preventing the protruding members formed on the cover and outer chamber from perforating the sealing members that seal the upper and lower openings of the inner chamber due to the inner chamber moving up and down due to vibrations that occur during the production and distribution process of the product.

[0010] Another objective of the present invention is to provide a genome extraction device that solves the problem of cross-contamination between reagents due to capillary action occurring through the space between the double chambers through the unique design of the inner chamber (lower inner chamber).

[0011] Another objective is to provide a genome extraction device that has a structure to prevent capillary action and a unique inner chamber design (upper inner chamber) to prevent reagents from leaking out.

[0012] Another object of the present invention is to provide a genome extraction device in which the sealing member can be torn with little force due to the configuration of the first protruding member formed on the bottom surface of the outer chamber, and the perforated portion expands, allowing the reagent contained inside the inner chamber to smoothly flow out to the outside.

[0013] Another object of the present invention is to provide a genome extraction device in which a sloped portion is formed around a discharge hole through which a reagent is discharged, thereby enabling the reagent to be smoothly discharged through the discharge hole.

[0014] In addition, the purpose of this invention is to provide a genome extraction device that is easier to manufacture and solves the problem of unintentional narrowing of the flow path, by arranging a double-structured flow cover-pad between the outer chamber and the base plate, compared to conventional genome extraction devices that have only one pad arranged.

[0015] Another object of the present invention is to provide a genome extraction device in which a tightly sealed flow path is formed without leakage from the middle during the reagent transfer process by achieving a strong bond between the base plate, flow cover, pad and outer chamber.

[0016] Another objective of the present invention is to provide a genome extraction device that can maintain the performance of beads, which are vulnerable to moisture, for a long period of time by having a double chamber structure consisting of an outer chamber and a bead chamber in the bead chamber that contains the beads necessary for genome extraction and amplification.

[0017] Another object of the present invention is to provide a genome extraction device in which the performance of the beads is maintained by a dehumidifying unit located above the bead chamber even when the bead chamber is opened.

[0018] Another object of the present invention is to provide a genome extraction device that employs an amplification module in which a sufficient volume of extract liquid can be introduced by introducing pretreated extract liquid, which allows the air remaining inside the storage section to be easily expelled.

[0019] In addition, the amplification module has multiple storage compartments, each of which stores different primers and probes for genome amplification, and the purpose is to provide a genome extraction device that can diagnose several types of diseases through a single genome extraction.

[0020] Another object of the present invention is to provide a genome amplification module in which multiple extract liquid movement passages have the same volume, and extract liquid can be injected into all storage sections simultaneously, and the same amount of extract liquid can be injected.

[0021] Another object of the present invention is to provide a genome amplification module in which a branch space that is wider and deeper than the extract liquid movement passage is formed adjacent to the inlet, and after the branch space is filled with extract liquid, the extract liquid can be simultaneously injected along each extract liquid movement passage.

[0022] Another object of the present invention is to provide a genome amplification module in which a branch space is formed at a first end adjacent to an inlet, and a storage section is provided at a second end far away from the first end, thereby preventing the amplified products in each storage section from being pushed out into the moving path or flowing back into other storage sections depending on the heating temperature during the amplification process.

[0023] In addition, a space that is wider and deeper than other parts is formed at the connection point of the gas transfer passage to the storage part, so that even if bubbles are generated, they do not affect the storage part, thereby providing a genome amplification module that improves detection accuracy.

[0024] Another object of the present invention is to provide a genome amplification module in which the width and depth of the gas transfer passage are minimized, thereby minimizing the amount of extractant discharged through the gas transfer passage.

[0025] Another object of the present invention is to provide a genome extraction method using the genome extraction device described above. [Means for solving the problem]

[0026] To solve the above problems, one embodiment of the present invention provides an amplification module including a body, an inlet formed in the body through which an extract flows, a plurality of storage units connected to the inlet to store the inflowing extract, a first branch space communicating with the inlet, a plurality of extract transfer passages branching from the branch space and connecting the inlet and the plurality of storage units, an outlet formed in the body through which gas is discharged, and a plurality of gas transfer passages connecting the outlet and the plurality of storage units.

[0027] In one embodiment, the first branch space may include a first through-hole connected to the inlet and penetrating the body, and a first recess formed between the first through-hole and the plurality of extract transfer passages and recessed into one surface of the body.

[0028] In one embodiment, at least one of the width and depth of the first branch space may be wider or deeper than the width and depth of the extract transfer passage.

[0029] In one embodiment, the volumes of the plurality of extraction liquid transfer passages may be the same.

[0030] In one embodiment, the first branch space may be formed at a first end in the width direction of the amplification module, and the plurality of accommodating portions may be formed at a second end opposite the first end in the width direction of the amplification module.

[0031] In one embodiment, the gas supply device may further include a second branch space formed between the exhaust port and the plurality of gas transfer passages, and the second branch space may include a second through-hole connected to the exhaust port and penetrating the body, and a second recess formed between the second through-hole and the plurality of gas transfer passages and recessed into another surface of the body.

[0032] In an embodiment, the first recess and the second recess may have overlapping portions in a width direction of the body and may be recessed into the body.

[0033] In one embodiment, the container may further include sealing members attached to one surface and an opposite surface of the body to seal the plurality of receiving portions, the first branch space, the second branch space, the extract transfer passage, and the gas transfer passage from an external space.

[0034] In one embodiment, the space between the first recess and the sealing member and the space between the second recess and the sealing member may be independent spaces that do not communicate with each other.

[0035] In one embodiment, the gas transfer passage may be connected to the upper portion of the receiving portion, and a space that is wider and deeper than other portions may be formed at the connection point.

[0036] In one embodiment, at least one of the width and depth of the gas transfer passage may be narrower or lower than the width and depth of the extract transfer passage.

[0037] In one embodiment, the gas transfer passage may be formed through a combination of one or more of a first gas transfer passage having a first width and a first depth, a second gas transfer passage having a second width larger than the first width and the first depth, and a third gas transfer passage having a third width larger than the second width and a second depth deeper than the first depth.

[0038] In one embodiment, a number of pillars may be protruded from the gas passage.

[0039] In one embodiment, the gas transfer passages connected to the plurality of receiving portions may all have the same volume.

[0040] In one embodiment, one of the plurality of storage units may store probes and primers for amplifying a first target substance, and another of the storage units may store probes and primers for amplifying a second target substance different from the first target substance. [Effects of the Invention]

[0041] The genome extraction device according to the present invention has an inner chamber containing reagents necessary for genome extraction, which is separate from the outer chamber, and the top and bottom of the inner chamber are sealed, thereby solving the problem of reagents contained in a single chamber leaking out in conventional genome extraction devices.

[0042] In addition, the protruding members formed on the cover and outer chamber prevent the sealing members that seal the upper and lower openings of the inner chamber from being punctured when the inner chamber moves up and down due to vibrations that occur during the production and distribution process of the product.

[0043] Furthermore, the problem of cross-contamination between reagents is resolved by capillary action occurring through the space between the double chambers.

[0044] Furthermore, the structure for preventing capillary action prevents the reagent from leaking out.

[0045] In addition, the configuration of the first protruding member formed on the bottom surface of the outer chamber allows the sealing member to be torn with little force, expanding the perforated area and allowing the reagent contained inside the inner chamber to smoothly flow out.

[0046] In addition, a sloped portion is formed around the outlet hole through which the reagent is discharged, so that the reagent can be smoothly discharged through the outlet hole.

[0047] In addition, the dual-structure flow cover-pad is placed between the outer chamber and the base plate, which improves ease of manufacturing compared to conventional genome extraction devices that only have one pad, and solves the problem of unintentional narrowing of the flow path.

[0048] Furthermore, a strong bond is achieved between the base plate, flow cover, pad and outer chamber, so that a sealed flow path is formed without any intermediate leakage during the reagent transfer process.

[0049] In addition, the bead chamber containing the beads necessary for genome extraction and amplification has a double chamber structure consisting of an outer chamber and a bead chamber, which makes it possible to maintain the performance of the beads, which are sensitive to moisture, for a long period of time.

[0050] In addition, even if the bead chamber is opened, the performance of the bead is maintained by the dehumidifying unit located above the bead chamber.

[0051] Furthermore, by adding the pretreated extract, the air remaining inside the storage section can be easily expelled, and a sufficient volume of extract can be added to the amplification module.

[0052] In addition, the amplification module has a plurality of storage compartments, each of which stores different primers and probes for genome amplification, making it possible to diagnose several types of diseases through a single genome extraction.

[0053] Furthermore, the plurality of extract liquid transfer passages have the same volume, so that the extract liquid can be injected into all the storage sections at the same time, and the same amount of extract liquid can be injected.

[0054] In addition, a branch space wider and deeper than the extract liquid passage is formed adjacent to the inlet, and after the branch space is filled with the extract liquid, the extract liquid can be simultaneously injected along each extract liquid passage.

[0055] In addition, a branch space is formed at the first end adjacent to the inlet, and a storage section is provided at the second end far away from the first end, which makes it possible to prevent the amplified products in each storage section from being pushed out into the moving path or flowing back into other storage sections due to the heating temperature during the amplification process.

[0056] In addition, a space that is wider and deeper than other parts is formed at the connection point of the gas passage with the receiving part, so even if bubbles are generated, they do not affect the receiving part, improving detection accuracy.

[0057] In addition, the width and depth of the gas transfer passage are minimized, thereby minimizing the amount of extracted liquid discharged through the gas transfer passage. [Brief explanation of the drawings]

[0058] [Figure 1] 1 is a perspective view showing the overall appearance of a genome extraction device according to an embodiment of the present invention. [Figure 2] FIG. 2 is a perspective view of the genome extraction device of FIG. 1 as seen from another side. [Figure 3] FIG. 2 is an exploded perspective view of FIG. 1. [Figure 4] FIG. 10 is a diagram for explaining the coupling relationship between the outer chamber and the inner chamber. [Figure 5] 10A and 10B are diagrams illustrating the coupling relationship between the inner chamber and the safety clip. [Figure 6] FIG. 1 is a plan view of the outer chamber. [Figure 7] FIG. 4 is a cross-sectional view illustrating the coupling relationship between the inner chamber and the outer chamber. [Figure 8] 10 is an enlarged view illustrating a second protruding member formed on the bottom surface of the outer chamber. [Figure 9] FIG. 2 is a diagram for explaining the inner chamber in more detail. [Figure 10] FIG. 10 is a bottom perspective view for more specifically illustrating the cover. [Figure 11]FIG. 10 is an exploded perspective view for more specifically illustrating the flow cover and pad disposed between the base plate and the outer chamber. [Figure 12] FIG. 2 is an exploded perspective view for specifically explaining the configuration of the piston. [Figure 13] FIG. [Figure 14] FIG. 4 is a perspective view for more specifically explaining the base plate. [Figure 15] 1 is a cross-sectional view for specifically explaining a genome extraction device according to an embodiment of the present invention. [Figure 16] FIG. 2 is another cross-sectional view for specifically explaining the genome extraction device according to an embodiment of the present invention. [Figure 17] FIG. 1 is a diagram illustrating an amplification module according to a first embodiment of the present invention. [Figure 18] FIG. 1 is a diagram illustrating an amplification module according to a first embodiment of the present invention. [Figure 19] FIG. 1 is a diagram illustrating an amplification module according to a first embodiment of the present invention. [Figure 20] FIG. 1 is a diagram illustrating an amplification module according to a first embodiment of the present invention. [Figure 21] FIG. 10 is a diagram illustrating an amplification module according to a second embodiment of the present invention. [Figure 22] FIG. 10 is a diagram illustrating an amplification module according to a second embodiment of the present invention. [Figure 23] FIG. 10 is a diagram illustrating an amplification module according to a second embodiment of the present invention. [Figure 24] FIG. 10 is a diagram illustrating an amplification module according to a third embodiment of the present invention. [Figure 25] FIG. 10 is a diagram illustrating an amplification module according to a third embodiment of the present invention. [Figure 26] FIG. 10 is a diagram illustrating an amplification module according to a third embodiment of the present invention. [Figure 27] FIG. 10 is a diagram illustrating an amplification module according to a fourth embodiment of the present invention. [Figure 28]FIG. 10 is a diagram illustrating an amplification module according to a fourth embodiment of the present invention. [Figure 29] FIG. 10 is a diagram illustrating an amplification module according to a fourth embodiment of the present invention. [Figure 30] FIG. 2 is a plan view of the bead chamber. [Figure 31] FIG. 2 is a perspective view for more specifically explaining the configuration of a bead chamber. [Figure 32] FIG. 2 is a perspective view for more specifically explaining the configuration of a bead chamber. [Figure 33] FIG. 32 is a cross-sectional view of the bead chamber of FIG. 31. [Figure 34] 32 is a longitudinal cross-sectional view of the bead chamber of FIG. 31, illustrating the structure of the bead chamber coupled to the outer chamber. DETAILED DESCRIPTION OF THE INVENTION

[0059] In some cases, well-known structures and devices are omitted or shown in block diagram form, focusing on the core functions of each structure and device, in order to avoid obscuring the concepts of the present invention.

[0060] Throughout the specification, when a part "comprising" or "including" a certain element, this does not mean that it excludes other elements, but that it may further include other elements, unless otherwise specified. Furthermore, terms such as "unit," "machine," and "module" used in the specification refer to a unit that processes at least one function or operation, and this may be embodied in hardware, software, or a combination of hardware and software. Furthermore, in the context of describing the present invention (particularly in the context of the following claims), the terms "a" (or "an"), "one," "the," and similar related words may be used to include both the singular and the plural, unless otherwise specified in the specification or clearly contradicted by the context.

[0061] In describing embodiments of the present invention, if a detailed description of well-known functions or configurations is deemed to unnecessarily obscure the gist of the present invention, the detailed description will be omitted. Furthermore, the terms used below are defined in consideration of the functions in the embodiments of the present invention, and may vary depending on the intentions or practices of users or operators. Therefore, the definitions should be based on the overall content of this specification.

[0062] The present invention will now be described in detail with reference to the accompanying drawings.

[0063] 1 to 3, a genome extraction device 1000 according to an embodiment of the present invention includes an outer chamber 100, an inner chamber 200, a cover 300, a base plate 400, a safety clip 500, an amplification module 600, a piston 700, a driving unit 800, and a bead chamber 900.

[0064] The outer chamber 100 is partitioned by outer chamber partitions into a plurality of first spaces 101, 102, 103, 104, 105, 106, and 107. That is, the plurality of first spaces 101, 102, 103, 104, 105, 106, and 107 may be spaces independent from one another.

[0065] The first spaces 101, 102, 103, 104, 105, 106, and 107 may have an open top and a closed bottom. First discharge holes 121, 122, 123, 124, and 125 are formed through the bottoms of the first spaces 101, 102, 103, 104, and 105, respectively, and are spaced a first distance from the center of the outer chamber 100 and extend in the circumferential direction. Second discharge holes 126 and 127 are formed through the bottoms of the remaining first spaces 106 and 107, respectively, and are spaced a second distance from the center of the outer chamber 100 and extend in the circumferential direction. Discharge holes 128 and 129, which communicate with the amplification module 600, are formed through the bottoms of the spaces between the first spaces 106 and 107. Here, the first distance may be shorter than the second distance, but in other embodiments, the first distance may be longer than the second distance.

[0066] A reagent stored in an inner chamber 200 (described later) is poured into the plurality of first spaces 101, 102, 103, 104, and 105, and beads stored in a bead chamber 900 are poured into the remaining plurality of first spaces 106 and 107.

[0067] Piston insertion portions 108 into which pistons 700 are inserted are formed penetrating vertically at the centers of the plurality of first spaces 101, 102, 103, 104, 105, 106, and 107. When the pistons 700 are inserted into the piston insertion portions 108 and a driving unit (not shown) of the diagnostic device is coupled to the pistons 700 to move the pistons 700 up and down, the reagent (fluid) in the first spaces 101, 102, 103, 104, 105, 106, and 107 can enter and exit the fluid containing portion 701 inside the pistons 700. More specific details will be described later.

[0068] 4, the upper outer surface 100a of the outer chamber 100 is recessed toward the center while being connected to the upper portion of the lower outer surface 100b. The safety clip 500 is connected to the upper outer surface 100a of the outer chamber 100, and the boundary between the upper outer surface 100a and the lower outer surface 100b serves as a short jaw of the safety clip 500, allowing the safety clip 500 to maintain its connected position after being connected to the upper outer surface 100a. The safety clip 500 has a length that at least partially surrounds the upper outer surface 100a of the outer chamber 100 and includes an extending outer chamber connecting portion 510 and a handle 520 formed on one side of the outer chamber connecting portion 510.

[0069] When the safety clip 500 is engaged with the outer chamber 100, the cover 300 applies pressure to the inner chamber 200 engaged with the outer chamber 100, preventing the upper and lower openings of the inner chamber 200 from opening. A user can start the extraction process after grasping the handle 520 and removing the safety clip 500 from the outer chamber 100. In other words, when the safety clip 500 is engaged with the outer chamber 100, the reagent in the inner chamber 200 cannot be introduced into the outer chamber 100, and only when the safety clip 500 is removed from the outer chamber 100 can the reagent in the inner chamber 200 be introduced into the outer chamber 100.

[0070] 3-5, the construction of the safety clip 500 will be described in more detail.

[0071] Safety clip 500 includes an outer chamber mating portion 510 , a handle 520 , a top extension 530 and a side extension 540 .

[0072] The outer chamber connecting part 510 is engaged with the outer chamber 100 while surrounding at least a portion of the outer surface (specifically, the upper outer surface 100a) of the outer chamber 100. More specifically, the outer chamber connecting part 510 is engaged with the outer chamber 100 so as to surround the four outer surfaces of the outer chamber 100, but the extended ends of the outer chamber connecting part 510 are configured to be spaced apart from each other. As shown in FIG. 1 , when the safety clip 500 is engaged with the outer chamber 100, the extended end of the outer chamber connecting part 510 rests on one of the outer surfaces of the outer chamber 100, and the safety clip 500 can only be separated from the outer chamber 100 by a user grasping the safety clip 500 and applying an external force in one direction.

[0073] The handle 520 is a portion that extends outward from the outer chamber connecting portion 510 and is a portion that is grasped by a user to separate the safety clip 500 from the outer chamber 100 .

[0074] The upper extension 530 extends upward on one side of the outer chamber joint 510 , and the side extension 540 extends from the upper extension 530 toward the center of the outer chamber 100 .

[0075] The safety clip 500 according to the embodiment of the present invention is characterized in that a cover support member 541 is protruded from the upper surface of the side extension portion 540, and an inner chamber coupling portion 542 is protruded from the extension end of the side extension portion 540.

[0076] When the safety clip 500 is engaged with the outer chamber 100, the cover support member 541 prevents the protruding members 311, 312, 313, 314, 315, 316, and 317 formed on the bottom surface of the cover 300 from tearing (puncturing) the first sealing member S1 that seals the upper openings of the multiple second spaces 201, 202, 203, 204, and 205 of the inner chamber 200 and the third sealing member S3 that seals the upper opening of the bead chamber 900.

[0077] 15, when the safety clip 500 is engaged with the outer chamber 100 and the inner chamber 200, the contact between the protruding members 311, 312, 313, 314, 315, 316, and 317 and the first and third sealing members S1 and S3 is blocked. Therefore, when the safety clip 500 is engaged with the outer chamber 100 and the inner chamber 200, perforation of the inner chamber 200 and the bead chamber 900 is prevented, thereby preventing the reagent contained in the inner chamber 200 and the beads contained in the bead chamber 900 from leaking into the outer chamber 100.

[0078] The inner chamber coupling part 542 is coupled to the fixing part 230 of the inner chamber 200 when the safety clip 500 is engaged with the outer chamber 100. When the inner chamber coupling part 542 is coupled to the fixing part 230, the bottom surface of the inner chamber 200 is positioned at a predetermined distance from the bottom surface of the outer chamber 100, and therefore, the second sealing member S2 sealing the lower openings of the plurality of second spaces 201, 202, 203, 204, 205 is prevented from being torn by the protruding members 111, 112, 113, 114, 115 formed on the bottom surface of the outer chamber 100 (see FIG. 15).

[0079] In the attached drawings, the inner chamber coupling portion 542 is shown in the form of an engaging protrusion and the fixing portion 230 is shown in the form of a coupling groove that couples with the engaging protrusion, but in other embodiments, the inner chamber coupling portion 542 may be provided in the form of a coupling groove and the fixing portion 230 may be provided in the form of an engaging protrusion that couples with the coupling groove.

[0080] The outer chamber 100 (more specifically, the partition wall of the outer chamber) has a recessed seating portion 109 that provides a space for seating the fixing portion 230 of the inner chamber 200. The inner chamber 200 is fixed at a position spaced a predetermined distance from the bottom surface of the outer chamber 100 through a coupling structure with the safety clip 500, and the fixing portion 230 of the inner chamber 200 is seated and supported by the seating portion 109, thereby improving the fixing force.

[0081] 7, an insertion space 130 is recessed into the upper side of the inner wall of the outer chamber 100, and the coupling hook 240 of the inner chamber 200 may be coupled to the insertion space 130. A stopper 131 is formed on the upper side of the insertion space 130 and protrudes toward the inside of the outer chamber 100. Therefore, when the inner chamber 200 is not pressurized by the cover 300, the coupling hook 240 of the inner chamber 200 is positioned on the stopper 131, but when the inner chamber 200 is pressurized by the cover 300, the coupling hook 240 is inserted into the insertion space 130 via the stopper 131.

[0082] Referring to Figure 15, the coupling relationship between the outer chamber and the inner chamber according to another embodiment of the present invention will be described. In Figure 7, instead of the coupling hook 240 formed on the inner chamber 200, a locking protrusion 250 is provided that protrudes outward from the outer wall of the inner chamber 200, and the locking protrusion 250 engages with a stopper 131 formed on the inner wall of the outer chamber 100, partially restricting the downward movement of the inner chamber 200. When the safety clip 500 is removed from the outer chamber 100 and the inner chamber 200 is pressurized by the cover 300, the locking protrusion 250 is inserted into the insertion space 130 via the stopper 131, thereby piercing the second sealing member S2 that seals the multiple second spaces of the inner chamber 200 by the protruding member formed on the outer chamber 100.

[0083] The inner chamber 200 is divided by partition walls into a plurality of second spaces 201, 202, 203, 204, and 205. That is, the plurality of second spaces 201, 202, 203, 204, and 205 may be spaces independent from one another.

[0084] The upper and lower portions of the second spaces 201, 202, 203, 204, and 205 are open (i.e., the second spaces have upper and lower openings), and the upper portions are sealed by a first sealing member S1 and the lower portions are sealed by a second sealing member S2. The first sealing member S1 and the second sealing member S2 may be, for example, a film, but are not limited thereto, and may be a film made of any material that is impermeable to fluids.

[0085] Different reagents are poured into each of the second spaces 201, 202, 203, 204, and 205, and the reagents are poured into the second spaces after the second sealing member S2 seals the lower portions of the second spaces, and then the first sealing member S1 seals the upper portions of the second spaces, thereby completing the pouring of the reagents into the inner chamber 200.

[0086] Referring to FIG. 4, the inner chamber 200 includes an upper inner chamber 210 and a lower inner chamber 220 .

[0087] The upper inner chamber 210 is integrally formed and configured to be in close contact with the inner wall of the outer chamber 100 when combined with the outer chamber 100 .

[0088] The lower inner chamber 220 is connected to the upper inner chamber 210 and includes a portion that is bent (toward the inside in the radius direction) to be spaced apart from the inner wall of the outer chamber 100 when combined with the outer chamber 100 .

[0089] Since the present invention uses a double chamber structure consisting of an inner chamber and an outer chamber, there may be a risk of cross-contamination between reagents in the inner chamber 200 during operation. Cross-contamination can occur due to capillary action occurring through the minute space between the inner and outer chambers, but in order to prevent this cross-contamination problem, the present invention employs a curved structure in which the inner chamber 200 is sufficiently spaced from the inner wall of the outer chamber 100 to prevent capillary action.

[0090] In addition, the outer chamber 100 and the inner chamber 200 are designed to be separated to prevent capillary action, and the upper inner chamber 210 is configured to be in close contact with the inner wall of the outer chamber 100 to prevent the reagent from leaking out through the separated area.

[0091] Meanwhile, first protruding members 111, 112, 113, 114, and 115 are protruded from the bottom surfaces of the first spaces 101, 102, 103, 104, and 105 to allow the reagent contained in the inner chamber 200 to flow into the first spaces 101, 102, 103, 104, and 105 by tearing the second sealing member S2 of the inner chamber 200.

[0092] Each of the first protruding members 111, 112, 113, 114, and 115 is arranged to correspond one-to-one to the plurality of first spaces 101, 102, 103, 104, and 105. For example, the first protruding member corresponding to drawing reference number 111 tears the second sealing member S2 sealing the upper part of the first space corresponding to drawing reference number 101, and the first protruding member corresponding to drawing reference number 115 tears the second sealing member S2 sealing the upper part of the first space corresponding to drawing reference number 105.

[0093] The first protruding members 111, 112, 113, 114, 115 include protruding portions 111a, 112a, 113a, 114a, 115a that protrude a first height (h1) from the bottom surfaces of the plurality of first spaces 101, 102, 103, 104, 105, and wing portions 111b, 112b, 113b, 114b, 115b that extend from the protruding portions 111a, 112a, 113a, 114a, 115a and protrude a second height (h2) that is lower than the first height (h1) from the bottom surfaces. Here, the wing portions 111b, 112b, 113b, 114b, 115b may have a structure that extends in both left and right directions from the protruding portions 111a, 112a, 113a, 114a, 115a.

[0094] The protrusion serves to pierce the second sealing member S2, and the wing serves to expand the perforation of the second sealing member S2. In the present invention, since the height of the protrusion is higher than the wing, point contact is made between the protrusion and the second sealing member S2 that seals the lower part of the inner chamber 200. This point contact has the effect of minimizing pressure when the second sealing member S2 is torn. Therefore, the second sealing member S2 can be torn with less force.

[0095] When the second sealing member S2 is torn by the protruding members 111, 112, 113, 114, and 115, the reagent stored in the second spaces 201, 202, 203, 204, and 205 of the inner chamber 200 flows out into the first spaces 101, 102, 103, 104, and 105 of the outer chamber 100. The leaked reagent is then discharged through the first discharge holes 121, 122, 123, 124, and 125 formed on the bottom surfaces of the first spaces 101, 102, 103, 104, and 105. In order to facilitate the outflow of reagent to the first discharge holes 121, 122, 123, 124, and 125, there are portions around the first discharge holes 121, 122, 123, 124, and 125 that are inclined downward toward the first discharge holes 121, 122, 123, 124, and 125. The inclined portions may have an angle of 3 to 10 degrees, which facilitates the process of the reagent that has flowed into the first spaces 101, 102, 103, 104, and 105 flowing out to the first discharge holes 121, 122, 123, 124, and 125.

[0096] The cover 300 is coupled to the top of the outer chamber 100 and is configured to cover the top of the outer chamber 100 and the inner chamber 200 .

[0097] Referring to FIG. 10, the cover 300 includes a cover body 301 and a lid 302 .

[0098] The cover body 301 is formed with a first insertion hole 307 aligned with the piston insertion portion 108 and a first specimen insertion hole 309 through which a specimen is inserted, and second protruding members 311, 312, 313, 314, and 315 for tearing the first sealing member S1 and third protruding members 316 and 317 for tearing the third sealing member S3 protruding from the bottom surface of the cover body 301.

[0099] The second protruding members 311, 312, 313, 314, and 315 are arranged to correspond one-to-one with the plurality of first spaces 101, 102, 103, 104, 105, 106, and 107, and the third protruding members 316 and 317 are arranged to correspond one-to-one with the plurality of third spaces 910 and 920. For example, the second protruding member corresponding to reference numeral 311 tears the first sealing member S1 sealing the upper part of the first space corresponding to reference numeral 101, and the second protruding member corresponding to reference numeral 315 tears the first sealing member S1 sealing the upper part of the first space corresponding to reference numeral 105.

[0100] A spacing member 320 is formed on the bottom surface of the cover body 301 along the periphery of the first insertion hole 307. The spacing member 320 separates the first protruding member and the first sealing member from each other when the safety clip 500 is engaged with the outer chamber 100. That is, the spacing member 320 is supported by the cover support member 541, so that the cover 300 is spaced a predetermined distance from the inner chamber 100.

[0101] The cover 302 is hingedly connected to one side of the cover body 301. A second insertion hole 308 aligned with the first insertion hole 307 is formed through the center of the cover 302.

[0102] When the safety clip 500 is separated from the outer chamber 100 while the cover 300 is engaged with the outer chamber 100, and the cover 300 is then pressed downward, the inner chamber 200 engaged with the outer chamber 100 descends along the inner wall of the outer chamber 100. First protruding members 111, 112, 113, 114, 115, 116, and 117 are formed on the bottom surface of the outer chamber 100, and second protruding members 311, 312, 313, 314, and 315 and third protruding members 316 and 317 are formed on the bottom surface of the cover 300. The protruding members tear the first sealing member S1 and the second sealing member S2 that seal the upper and lower openings of the inner chamber 200, and the third sealing member S3 that seals the upper opening of the bead chamber 900. Therefore, the reagent contained in the inner chamber 200 flows out into the multiple first spaces 101, 102, 103, 104, and 105 of the outer chamber 100, and the second sealing member S2 sealing the upper opening of the inner chamber 200 tears, thereby acting as an air vent to ensure that the reagent is sufficiently discharged into the first spaces.

[0103] The base plate 400 is coupled to the bottom of the outer chamber 100 and includes a plurality of channels that guide the path along which the reagent travels between the first spaces 101 , 102 , 103 , 104 , 105 , 106 , 107 of the outer chamber 100 and the fluid-containing portion of the piston 700 .

[0104] According to one embodiment of the present invention, the base plate 400 may have a liquid flow path through which liquid can move and an air flow path through which air can move, and a flow cover 410 and a pad 420 may be further provided on the upper surface of the base plate 400 between the outer chamber 100 and the base plate 400 to prevent leakage of liquid when the outer chamber 100 is coupled. When the base plate 400-flow cover 410-pad 420 are coupled, the upper surfaces of the liquid flow path and the air flow path of the base plate 400 are filled with the flow cover 410 and the pad 420, forming a space and completing the flow path.

[0105] The liquid flow path is connected to the flow cover 410, the pad 420 and the outer chamber 100 to provide a space in which the sample and reagents can move and mix.

[0106] The air flow path connects the amplification module 600 and the vacuum control portion of the piston 700, and serves to control the vacuum that may be generated when the genome extracted from the amplification module 600 moves, thereby preventing contamination of the amplification products that may be generated during genome amplification.

[0107] A plurality of flow channels 401, 402, 403, 404, 405, 406, 407, 408, and 409 are formed on the upper portion of the base plate 400. The flow channels do not intersect with each other and extend from the center to the outer periphery of the base plate 400. Here, the liquid flow channels are components corresponding to reference numerals 401 to 408, and the air flow channel is a component corresponding to reference numeral 409.

[0108] Referring to FIG. 14, some of the multiple flow paths may have one end arranged on the same circumference, and the other ends may also be arranged on the same circumference.

[0109] A piston driver insertion hole 400a is formed through the center of the base plate 400 so that a piston driver 800 for rotating the piston 700 can be coupled thereto.

[0110] A flow cover 410 is placed in the mounting space on the base plate 400. The flow cover 410 may be made of, for example, plastic, and may be ultrasonically fused to the upper part of the base plate 400 while being mounted thereon, thereby being integral with the base plate 400.

[0111] The flow cover 410 has a first through hole 410a aligned with the piston drive unit insertion hole 400a, and has a plurality of first flow cover holes 411a, 412a, 413a, 414a, 415a, 416a, 417a, and 418a formed therethrough on a first circumference a first distance away from the first through hole 410a, a plurality of second flow cover holes 411b, 412b, 413b, 414b, and 415b formed therethrough on a second circumference a second distance away from the first through hole 410a, a plurality of third flow cover holes 416b, 417b, and 418b formed therethrough on a third circumference a third distance away from the first through hole 410a, and fourth flow cover holes 419a and 419b connected to one end and the other end of the air flow path 409. Here, the first flow cover hole is aligned with one inner end of the flow path formed in the body plate 400, the second flow cover hole and the third flow cover hole are aligned with the other outer ends of the flow path, and the fourth flow cover hole communicates with one end and the other end of the air flow path. The second distance may be longer than the first distance or shorter than the third distance.

[0112] Referring to FIG. 11, a first engaging protrusion 410b protruding upward and downward may be further formed on the outer periphery of the first through-hole 410a.

[0113] Also, melt protrusions 410c may be formed on the bottom surface of the flow cover 410 to bond along the edges of the multiple flow channels of the base plate 400 (see Fig. 12). When ultrasonic welding is performed after the flow cover 410 is placed on the top surface of the base plate 400, the melt protrusions 410c melt and become integrated with the base plate 400. This allows for tight bonding between the base plate 400 and the flow cover 410.

[0114] A pad 420 is placed on the flow cover 410. The pad 420 may be made of, for example, a silicon material, but is not limited thereto and may be made of any material having a predetermined elasticity.

[0115] The flow cover 410 has a plurality of second engagement protrusions 410d formed protruding from the upper surface thereof, and the second engagement protrusions 410d are coupled to the coupling grooves 420c of the pad 420, thereby achieving a strong coupling between the flow cover 410 and the pad 420. In addition, the first engagement protrusions 410b of the flow cover 410 are also inserted into the second through-holes 420a of the pad 420, thereby achieving a strong coupling between the two components.

[0116] The pad 420 has a second through hole 420a aligned with the first through hole 410a, and a plurality of first pad holes 421a, 422a, 423a, 424a, 425a, 426a, 427a, and 428a formed therethrough on a first circumference a first distance away from the second through hole 420a, a plurality of second pad holes 421b, 422b, 423b, 424b, and 425b formed therethrough on a second circumference a second distance away from the second through hole 420a, a plurality of third pad holes 426b, 427b, and 428b formed therethrough on a third circumference a third distance away from the second through hole 420a, and fourth pad holes 429a and 429b communicating with one end and the other end of the air flow path 409 are formed therethrough. Here, the first pad hole is aligned with the first flow cover hole, the second pad hole is aligned with the second flow cover hole, the third pad hole is aligned with the third flow cover hole, and the fourth pad hole is aligned with the fourth flow cover hole.

[0117] The pad 420 has a protrusion formed on its upper surface that protrudes from the area where the plurality of second pad holes 421b, 422b, 423b, 424b, and 425b, the plurality of third pad holes 426b, 427b, and 428b, and the fourth pad hole 429b communicating with the other end of the air flow path are formed, and that narrows toward the top. The protrusion solves the problem of the diameter of the pad hole unintentionally decreasing even when the pad 420 is closely placed between the outer chamber 100 and the base plate 400.

[0118] The amplification module 600 is engaged with the outer chamber 100 and is configured to receive a pre-treated sample. The pre-treatment of the sample means that genomes such as DNA and RNA contained in the sample have been lysed into a reagent. When the genome extraction device 1000 according to the present invention is coupled to a diagnostic device (not shown), an amplification process (e.g., PCR) of the genome received in the amplification module 600 is performed.

[0119] 1 and 2, the amplification module 600 is engaged vertically with the outer chamber 100. In other words, the amplification module 600 is engaged with the outer chamber 100 so that the upper portion 631 of the housing portion 630 is further away from the ground than the lower portion 632.

[0120] 17 to 29, the amplification module 600 includes a body 610, an inlet 621, an outlet 622, a storage section 630, a gas transfer passage 640, and an extract transfer passage 650.

[0121] The body 610 is a part that forms the outer shape of the amplification module 600 , and a first end 613 of the body 610 is formed with an inlet 621 and an outlet 622 that are connected to the outlet holes 128 and 129 of the outer chamber 100 .

[0122] The inlet 621 is connected to the discharge hole 129 and serves as an inlet for the extract discharged from the discharge hole 128 to be introduced into the storage section 630, and the outlet 622 is connected to the discharge hole 128 and serves as an outlet for the extract to be introduced into the amplification module 600 and the internal air to be discharged into the air flow path of the extraction device 1000.

[0123] That is, when the amplification module 600 is coupled to the extraction device 1000 , the inlet 621 communicates with the liquid flow path 408 and the outlet 622 communicates with the air flow path 409 .

[0124] A receiving section 630 is formed at a position farther from the body 610, the second end 614, the inlet 621 and the outlet 622 than the extracting device 1000, and serves as a space for receiving the extract flowing in through the inlet 621.

[0125] In one example, the receiving portion 630 is manufactured in a form that penetrates one side of the body 610 and the opposite side thereof completely, but in another example, it may be manufactured in a form that penetrates only one side and does not penetrate the opposite side. Both of the above embodiments are the same in that the open portions are sealed with sealing members S4 and S5. Therefore, the extract and air are introduced into and discharged from the receiving portion 630 only through the gas transfer passage 640 and the extract transfer passage 650.

[0126] According to an embodiment of the present invention, one or more receiving units 630 may be provided in one amplification module 600. Figures 17 to 23 show an amplification module having three receiving units, and Figures 24 to 29 show an amplification module having four receiving units.

[0127] The receiving portion 630 may have a substantially trapezoidal shape, and more specifically, it is preferable that the receiving portion 630 has a trapezoidal shape with rounded edges.

[0128] Here, the trapezoidal shape refers to a shape in which the width narrows as it becomes farther away from the gas transfer passage 640 and the extract transfer passage 650. The above-described shape of the receiving portion 630 solves the problem of bubbles being generated when the extract is injected through the extract transfer passage 650. If bubbles remain in the amplification module 600, particularly in the receiving portion 630, this can cause a problem of low analytical accuracy in the fluorescence detection process after the amplification process, and the shape of the receiving portion 630 can solve this problem. In the present invention, the amplification process may include an isothermal amplification process (LAMP) and a real-time nucleic acid amplification reaction (Real-time Polymerase Chain Reaction), but is not particularly limited thereto as long as it is a process for amplifying a genome.

[0129] Primers and probes necessary for genome amplification are stored in the storage unit 630. The amplification module 600 according to an embodiment of the present invention may include one or more storage units 630, each containing primers and probes targeting different substances. Therefore, multiple viruses / diseases can be simultaneously detected in the genome extracted from a single sample. For example, one storage unit 630 may contain primers and probes for amplifying coronaviruses, and another storage unit 630 may contain primers and probes for amplifying influenza viruses. This allows different viruses / diseases to be simultaneously detected in a single amplification process in a single amplification module 600.

[0130] The gas transfer passage 640 is formed on one surface 611 of the body 610 and is configured to connect the outlet 622 to the upper part 631 of the receiving part 630. Conversely, the extract transfer passage 650 is formed on the opposite surface 612 opposite to the one surface 611 and is configured to connect the inlet 621 to the lower part 632 of the receiving part 630.

[0131] First, the extract transfer path 650 will be described with reference to Figures 20, 23, 26 and 29. As described above, the extract transfer path 650 serves as a path through which the extract pretreated in the genome extraction device 1000 moves.

[0132] In this embodiment of the present invention, the number of extract transfer passages 650 is the same as the number of receiving units 630. In other words, in the case of an amplification module 600 having three receiving units 630 as shown in Fig. 20, three extract transfer passages 650 are provided, and in the case of an amplification module 600 having four receiving units 630 as shown in Fig. 29, four extract transfer passages 650 are provided.

[0133] The extract transfer passages 650 are configured to have the same volume. For example, the extract transfer passages 650 may all have the same length, width, and depth. Even if one or more of the depth, width, and depth differ, the volume of each passage is the same. Therefore, the extract developed along the extract transfer passages 650 reaches the storage units 630 simultaneously, so that all storage units 630 are filled with the extract.

[0134] Meanwhile, in order to minimize the generation of bubbles during the process of the extract spreading through the extract transfer passage 650, the extract transfer passage 650 has curved connecting portions without sharp edges, thereby minimizing the generation of bubbles.

[0135] A first branch space 660 is formed between the extract transfer passage 650 and the inlet 621. The first branch space 660 allows the inlet 621 and the extract transfer passage 650 to communicate with each other.

[0136] Referring to FIG. 20, the first branch space 660 includes a first through-hole 661 and a first recess 662 .

[0137] The first through-hole 661 is configured to penetrate the body 610 while being connected to the inlet 621, and the first recess 662 is formed between the first through-hole 661 and the extract transfer passage 650. It does not penetrate the body 610 but is recessed into one surface 611. As will be described later, a second recess 672 is also formed on the opposite surface 612 of the body 610, and the first recess 662 and the second recess 672 are formed at positions where they partially overlap in the width direction. However, the space between the first recess 662 and the sealing member and the space between the second recess 672 and the sealing member partially overlap to form independent spaces that do not communicate with each other. If the first recess 662 and the second recess 672 are configured to penetrate the body 610, the extract spreading into the extract passage 650 through the inlet 621 may also spread into the gas passage 640, and conversely, a problem may occur in that air discharged from the storage section 630 toward the outlet 622 may also spread into the extract passage 650. Therefore, in the present invention, the first recess 662 and the second recess 672 are configured as a recess rather than a penetration. On the other hand, the first penetration section and the second penetration section, which do not overlap each other in the width direction, do not cause interference problems even if they are formed as a penetration section.

[0138] Each of the extract transfer passages 650 starts from the first recess 662 and extends to the receiving portion 630, bending one or more times.

[0139] The width and depth of the first through-hole 661 and the first recess 662 are greater than the width and depth of the extract transfer passage 650. Therefore, assuming that the extract transfer passage 650, the first through-hole 661, and the first recess 662 have the same length, the capacity of the first through-hole 661 and the first recess 662 is greater than that of the extract transfer passage 650.

[0140] That is, the extract liquid injected through the inlet 621 spreads more slowly in the first branch space 660 than in the extract liquid moving passage 650, resulting in a kind of stagnation. After the first branch space 660 is completely filled with extract liquid, the extract liquid spreads in the extract liquid moving passage 650, which has the advantage that the extract liquid can spread simultaneously in each extract liquid moving passage 650 compared to when the first branch space 660 is not provided (in the case of an amplification module without a first branch space, the extract liquid is first injected into the extract liquid moving passage located at the bottom due to gravity).

[0141] Meanwhile, it is preferable that the first branch space 660 is formed at a first end 613 in the width direction of the amplification module 600, and the receiving portion 630 is formed at a second end 614 opposite to the first end 613. In other words, it is preferable that the receiving portion 630 and the first branch space 660 are spaced apart from each other in the width direction.

[0142] Extraction liquid is introduced into and contained in the container 630, and amplification reaction is carried out by bringing a heating device / cooling device into proximity or contact with the container 630. In order to prevent the amplified product in each container 630 from being pushed out into the transfer paths 640 and 650 or flowing back into other containers 630 due to the heating temperature during the amplification process, a first branch space 660 and a second branch space 670 (described later) are located at the first end 613 adjacent to the inlet 621 and outlet 622.

[0143] The gas transfer passage 640 serves as a passage through which gas (e.g., air) moves within the receiving section 630. The channel of the amplification module 600 is connected to the channel of the genome extraction device 1000 and generally has the characteristics of a closed channel. Because the receiving section 630 is filled with air before the extraction liquid is injected, an appropriate volume of air must be discharged to the outside once the extraction liquid is injected. In the present invention, the air inside the amplification module 600 is discharged to the air channel 409 via the gas transfer passage 640 through the outlet 622, thereby preventing excessive pressure buildup within the receiving section 630 caused by the injection of the extraction liquid and solving the problem of bubbles caused by air remaining inside. Like the receiving section 630, the gas transfer passage 640 preferably has a curved connection without any angular portions to minimize the generation of bubbles.

[0144] Gas is lighter than liquid such as extract, and in the present invention, a plurality of gas transfer passages 640 are connected to the end of the upper portion 631 of the receiving portion 630. At the connection point of the gas transfer passage 640 to the receiving portion 630, there is located an air bubble receiving portion 633 that is wider and deeper than the other portions of the gas transfer passage 640. Even if air bubbles are generated inside as the extract is poured, the air bubbles are contained in the wide and deep air bubble receiving portion 633 / 643, and therefore the air bubbles do not affect the receiving portion 630.

[0145] FIG. 25 shows a first embodiment of an amplification module having four receiving portions 630, and FIG. 28 shows a second embodiment of an amplification module having four receiving portions 630. As shown in FIG.

[0146] Both of the two embodiments are characterized by the fact that the width and depth of the gas transfer passage 640 are minimized, so that the inflow of liquid into the gas transfer passage 640 is minimized and only air can pass through.

[0147] The gas transfer passage 640 of the amplification module 600 according to the first embodiment is configured by a combination of a first gas transfer passage 641 having a first width and a first depth, a second gas transfer passage 642 having a second width larger than the first width and the first depth, and a third gas transfer passage 643 having a third width larger than the second width and a second depth deeper than the first depth. Specifically, the amplification module 600 according to the first embodiment is configured so that the volumes of each gas transfer passage 640 are all the same.

[0148] On the other hand, the first gas transfer passage 641, which is the narrowest and deepest of the gas transfer passages 640 of the amplification module 600 according to the first embodiment, may have a width of 0.135 mm to 0.165 mm, more specifically 0.14 mm to 0.16 mm, even more specifically 0.145 mm to 0.155 mm, preferably 0.15 mm, and a depth of 0.045 mm to 0.055 mm, more specifically 0.0475 mm to 0.0525 mm, preferably 0.05 mm.

[0149] The gas transfer passage 640 of the amplification module 600 according to the second embodiment may have a constant width and depth along its length. The width may be 0.45 mm to 0.55 mm, more specifically 0.475 mm to 0.525 mm, even more specifically 0.49 mm to 0.51 mm, and preferably 0.5 mm, and the depth may be 0.045 mm to 0.055 mm, more specifically 0.0475 mm to 0.0575 mm, even more specifically 0.049 mm to 0.051 mm, and preferably 0.05 mm. The gas transfer passage 640 of the amplification module 600 according to the second embodiment is provided with a number of protruding pillars (r) formed through a laser patterning process. This further minimizes the width and depth of the gas transfer passage 640, minimizing the inflow of liquid and allowing only air to pass through. Similar to the first embodiment, the amplification module 600 according to the second embodiment is also configured so that the volumes of the gas transfer passages 640 are all the same.

[0150] In this embodiment of the present invention, the number of gas transfer passages 640 is the same as the number of receiving portions 630. In other words, in the case of an amplification module 600 having three receiving portions 630 as shown in Figures 19 and 22, three gas transfer passages 640 are provided, and in the case of an amplification module 600 having four receiving portions 630 as shown in Figures 25 and 28, four gas transfer passages 640 are provided.

[0151] The piston 700 is inserted into the piston insertion portion 108 of the outer chamber 100 and is configured to move up and down to suck in the reagent contained in the outer chamber 100 or to expel the reagent sucked into the outer chamber 100 or the amplification module 600.

[0152] 3 and 12, the piston 700 includes an upper piston 710 and a lower piston 720.

[0153] The upper piston 710 has an open top and a fluid receiving portion 701 formed therein to receive the drawn fluid. A sealing portion 711 is installed inside the upper piston 710. The outer surface of the sealing portion 711 is in close contact with the inner surface of the upper piston 710, so that fluid cannot enter or exit through the space between the outer surface of the sealing portion 711 and the inner surface of the upper piston 710. A drive unit mounting portion 711a is recessed in the center of the sealing portion 711 to which a drive unit (not shown) of a diagnostic device is connected. The drive unit (not shown) of the diagnostic device is connected to the drive unit mounting portion 711a, and moves the sealing portion 711 up and down inside the upper piston 710 to draw fluid into the fluid receiving portion 701 or discharge the fluid contained in the fluid receiving portion 701 to the outside.

[0154] A coupling structure that engages with the lower piston 720 is formed on the bottom surface of the upper piston 710, and a first hole 712 that is connected to the liquid port of the lower piston 720 and a second hole 713 that is connected to the filter port of the lower piston 720 are formed through the bottom surface of the upper piston 710. The second hole 713 may be formed to have a smaller diameter than the filter seating space of the filter port to prevent separation of the support structure and the filter.

[0155] The lower piston 720 is fixed by engaging with a coupling structure formed on the bottom surface of the upper piston 710 .

[0156] The lower piston 720 may include a disk-shaped body 721, a shaft 722 formed to protrude outward from the center of the body 721, and a liquid port 723 and a filter port 724 arranged at the same distance from the center of the body 721.

[0157] The liquid port 723 is used to suck, mix and discharge the sample and reagent into the piston 700, and the filter port 724 is used to wash the genome collection filter and separate the genome from the genome collection filter.

[0158] In addition, a groove recessed toward the center may be formed on the outer periphery of the body 721 of the lower piston 720. The groove serves to remove vacuum that may occur when liquid moves inside the extractor.

[0159] Liquid port 723 and filter port 724 are arranged on the same circumference, spaced apart by a fixed angle. For example, filter port 724 and liquid port 723 may be arranged 18 to 36 degrees apart, and more specifically, the two ports may be arranged at an interval of 22.5 degrees. When using a step motor that rotates once in 16 steps, the positions of liquid port 723 and filter port 724 can be changed with a single drive.

[0160] The filter port 724 of the lower piston 720 may include a filter seating space 725, and a filter and a support structure may be placed in the filter seating space 725. The filter for genome capture may be a glass fiber filter having various particle sizes or a mold fixture, and the support structure serves to fix the filter for genome capture.

[0161] The support structure can be made of a porous plastic material with a certain granularity to prevent the filter from coming off when fluid is discharged and to maintain a certain pressure.

[0162] The driving unit 800 is connected to a driving unit (not shown) of the diagnostic equipment and serves as a mediator for rotating the piston 700 at a certain angle.

[0163] The driving unit 800 may include an engagement groove formed in the center of one surface to engage with the shaft 722, and a driving groove formed on the other surface to engage with a driving unit (not shown) of a diagnostic device.

[0164] The driving unit 800 is coupled to the piston 700 to position the liquid port 723 and the filter port 724 at the appropriate position of the first discharge hole of the outer chamber 100 so that the various chemical reactions required for the genome extraction step can be performed within a single device.

[0165] The liquid port 723 and the filter port 724 are spaced apart at a certain angle, and the driving unit 800 rotates the ports to positions appropriate for each stage during genome extraction.

[0166] The bead chamber 900 includes a first bead chamber 910, a second bead chamber 920, and a dehumidification chamber 930, which are separated by a first bead chamber partition wall 901 and a second bead chamber partition wall 902. The first bead chamber 910 is inserted into the first space 106 of the outer chamber 100, and the second bead chamber 920 is inserted into the first space 107 of the outer chamber 100.

[0167] Similar to the inner chamber 200, the upper opening of the bead chamber 900 is also sealed by a third sealing member S3, which is pierced by third protruding members 316 and 317 formed on the bottom surface of the cover 300 when the cover 300 is engaged with the outer chamber 100. Since the upper opening of the bead chamber 900 is opened by the third protruding members 316 and 317, even if a fluid is subsequently introduced into the first bead chamber 910 and the second bead chamber 920, a corresponding amount of air can be discharged through the pierced portion.

[0168] The bottom opening of the bead chamber 900 is open and not sealed with a separate sealing member. The bead chamber 900 stores dry beads (more specifically, freeze-dried beads), which are vulnerable to moisture. In the genome extraction device according to the present invention, the bottom opening of the bead chamber 900, the first space of the outer chamber 100, the flow cover 410, the pad 420, the channels of the base plate 400, and the channels of the amplification module 600 are connected to each other but form closed channels that are not exposed to the outside air, thereby minimizing the inflow of moisture into the bead chamber 900.

[0169] The first bead chamber 910 may store several dry beads (b1) required for genome extraction, and the second bead chamber 920 may store several dry beads (b2) required for genome amplification.

[0170] A first bead holder 911 configured to keep dry beads (b1) inside without being discharged to the outside is installed at the top opening of the first bead chamber 910, and a first dehumidifying unit 912 for dehumidifying the internal space of the first bead chamber 910 is installed in the dehumidifying chamber 930. Here, the dry beads required for genome amplification are provided in the form of, for example, capsules, but are not limited thereto.

[0171] A second bead holder 921 configured to keep dry beads (b2) inside without being discharged to the outside is installed at the upper opening of the second bead chamber 920, and a second dehumidifying unit 922 that dehumidifies the inside of the second bead chamber 920 is installed above the second bead holder 921. A third sealing member S3 seals the second bead chamber 920 so that it does not communicate with the dehumidifying chamber 930 and the first bead chamber 910, but seals the first bead chamber 910 so that they communicate with each other. This will be described in detail with reference to Figures 30 and 31.

[0172] The above-mentioned effect is achieved by configuring the height difference between the first bead chamber partition wall 901 and the second bead chamber partition wall 902. Referring to Figures 30 and 31, the second bead chamber partition wall 902, which separates the second bead chamber 920 and the dehumidifying chamber 930, has a height greater than the first bead chamber partition wall 901, which separates the first bead chamber 910 and the dehumidifying chamber 930.

[0173] In other words, the top of the second bead chamber partition 902 extends to the same height as the top of the outer partition that forms the second bead chamber 920, and the top of the first bead chamber partition 901 extends to a lower height than the top of the outer partition that forms the first bead chamber 910.

[0174] Therefore, even if the upper opening of the bead chamber 900 is sealed by the third sealing member S3, the first bead chamber 910 and the dehumidifying chamber 930 can communicate with each other through the space between the first bead chamber partition wall 901 and the third sealing member S3. Therefore, the first bead chamber 910 is dehumidified by the second dehumidifying unit 922 installed inside the dehumidifying chamber 930.

[0175] The lower opening 914 of the first bead chamber 910 (i.e., the discharge port of the first bead chamber) and the lower opening 924 of the second bead chamber 920 (i.e., the discharge port of the second bead chamber) are formed at the ends of discharge passages 913, 923 that narrow from the bead chamber 900 toward the base plate 400.

[0176] Dry beads may be accommodated inside the discharge passages 913 and 923, and a bead holder may be installed at the top of the discharge passages 913 and 923 to prevent the beads accommodated in the discharge passages 913 and 923 from leaking out.

[0177] The discharge passages 913, 923 may have a tapered shape that narrows toward the base plate 400. The diameter of the lower openings 914, 924 located at the ends of the discharge passages 913, 923 is smaller than the diameter of the dry beads, so that the beads are not discharged to the outside through the lower openings 914, 924. Fluid flows into the inside of the discharge passages 913, 923 through the lower openings 914, 922, dissolves the dry beads, and is discharged only in the form of fluid through the lower openings 914, 924 to the fluid container of the external piston or the amplification module.

[0178] Here, the discharge passage 913 of the first bead chamber 910 in which dry beads necessary for genome amplification are stored may have a wider diameter than the discharge passage 923 of the second bead chamber 920 and may become narrower toward the base plate 400.

[0179] The first bead chamber 910 is the structure into which the final fluid is introduced before the pre-treated extract is introduced toward the amplification module 600. Accurate detection results can only be obtained if the fluid introduced into the first bead chamber 910 is not left behind in the first bead chamber 910 as much as possible and is introduced into the receiving part 630 of the amplification module 600. Therefore, in the present invention, the discharge passage 913 of the first bead chamber 910 has a wider diameter than the discharge passage 923 of the second bead chamber 920, and is formed to be narrower, thereby minimizing the amount of fluid remaining in the first bead chamber 910.

[0180] The bead chamber 900 according to the present invention also has first locking protrusions 903 and 904 extending from the bottom surfaces of the outer partition walls of the first bead chamber 910 and the second bead chamber 920. As shown in Figures 32 and 34, the first locking protrusions 903 and 904 may be formed in a structure that extends toward the base plate 400 and then protrudes outward.

[0181] The outer chamber 100, which is connected to the bead chamber 900, has a second locking protrusion 109a formed on one side of the outer chamber partition wall that divides the first spaces. When a force is applied to the bead chamber 900 toward the base plate 400, the first locking protrusions 903 and 904 are connected to each other via the second locking protrusion 109a, thereby firmly connecting the two components. When the first locking protrusions 903 and 904 are connected to the second locking protrusion 109a, the relative position of the bead chamber 900 to the outer chamber 100 is fixed.

[0182] The extraction method according to the embodiment of the present invention will be specifically described below.

[0183] First, (a) the inner chamber is coupled to the outer chamber through the upper openings of the first spaces of the outer chamber, and the fixing portion of the inner chamber is preferably coupled to the outer chamber while being coupled to the inner chamber coupling portion of the safety clip.

[0184] Next, (b) the cover is coupled to the outer chamber, and (c) the safety clip is removed from the outer chamber.

[0185] Next, (d) the cover is pressurized, causing the first sealing member sealing the upper opening of the inner chamber to tear by the first protruding member formed on the bottom surface of the cover, and the second sealing member sealing the lower opening of the inner chamber to tear by the second protruding member formed on the bottom surface of the multiple first spaces of the outer chamber, causing the reagent contained in the inner chamber to flow out into the multiple first spaces, and (e) by driving the driving unit, the reagent that has flowed out into the multiple first spaces is sucked into and mixed in the fluid containing section inside the upper piston, and then the mixed reagent is discharged to the amplification module.

[0186] Step (e) may be performed in multiple steps, which will be described in more detail below.

[0187] First, (e1) a sample to be analyzed is introduced into one of the plurality of first spaces of the outer chamber through the sample introduction hole of the cover.

[0188] Next, (e2) the piston installed in the piston accommodating portion of the outer chamber rotates, and the liquid port of the piston communicates with the first discharge hole formed on the bottom surface of any one of the first spaces into which the sample to be analyzed is introduced.

[0189] Next, (e3) the contact part installed in the internal space of the piston rises, and the specimen to be analyzed accommodated in any one of the first spaces is sucked into the fluid accommodation part in the external chamber.

[0190] Next, (e4) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of another one of the first spaces.

[0191] Next, (e5) the contact portion rises and the first reagent contained in the other first space is sucked into the fluid containing portion inside the outer chamber, thereby mixing the sample to be analyzed and the first reagent in the fluid containing portion.

[0192] Next, (e6) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of another one of the first spaces.

[0193] Next, (e7) the contact portion rises and the second reagent contained in the other first space is sucked into the fluid containing portion inside the outer chamber, thereby mixing the sample to be analyzed with the first reagent and the second reagent.

[0194] Next, (e8) the piston rotates, and the filter port of the piston communicates with the first discharge hole formed in the bottom surface of the other first space.

[0195] Next, (e9) the contact part descends, and the mixed liquid contained in the fluid containing part passes through the genome collection filter installed in the filter port and is discharged into the other first space.

[0196] Next, (e10) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of the first space in which a reagent different from the first reagent and the second reagent is stored.

[0197] Next, (e11) the contact portion rises and other reagents are sucked into and mixed with the fluid containing portion.

[0198] Next, (e12) the piston rotates, and the filter port of the piston communicates with the first discharge hole formed in the bottom surface of the first space containing another reagent.

[0199] Next, (e13) the contact part descends, and the mixed liquid contained in the fluid containing part passes through the genome collection filter and is discharged into the first space containing other reagents.

[0200] Next, (e14) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of the first space containing the eluent.

[0201] Next, (e15) the contact portion rises and the eluent is sucked into the fluid storage portion.

[0202] Next, (e16) the piston rotates, and the filter port of the piston communicates with the second discharge hole formed in the bottom surface of the first space containing the beads necessary for genome amplification.

[0203] Next, (e17) the contact portion descends, and the eluent contained in the fluid containing portion passes through the genome collection filter and is discharged into the first space containing the beads necessary for genome amplification, and the genomes captured on the genome collection filter are separated from the genome collection filter and discharged together into the first space.

[0204] Next, (e18) the piston rotates, and the liquid port of the piston communicates with the second discharge hole formed in the bottom surface of the first space in which the genome is accommodated.

[0205] Next, (e19) the contact part rises and the extract containing the genome is sucked into the fluid storage part.

[0206] Next, (e20) the piston rotates, bringing the liquid port of the piston into communication with the amplification module.

[0207] Next, (e21) the contact part is lowered, and the extract containing the genome contained in the fluid containing part is discharged into the amplification module.

[0208] Next, (e22) the extract is introduced into the storage section of the amplification module through the extract transfer passage of the amplification module.

[0209] Next, (e23) the air remaining in the storage section is discharged to the outside of the amplification module through the gas transfer passage of the amplification module.

[0210] Next, (e24) the amplification device applies heat of a predetermined temperature or higher to the storage section, thereby amplifying the genome.

[0211] Next, (e25) the presence or absence of disease infection in the specimen to be analyzed is determined based on the fluorescence intensity of the amplified product of the genome.

[0212] Although the present invention has been described above with reference to the embodiments shown in the drawings so that those skilled in the art can easily understand and reproduce the present invention, these are merely illustrative examples, and those skilled in the art will understand that various modifications and equivalent embodiments are possible from the embodiments of the present invention. Therefore, the scope of protection of the present invention should be determined by the claims. [Explanation of symbols]

[0213] S1: First sealing member S2: Second sealing member S3: Third sealing member S4, S5: Sealing material 100: Outer chamber 100a: Upper outer surface 100b: Lower outer surface 101,102,103,104,105,106,107: 1st space 108: Piston insertion part 109: Landing section 109a: Second locking protrusion 111, 112, 113, 114, 115: First protruding member 111a, 112a, 113a, 114a, 115a: Protrusion 111b, 112b, 113b, 114b, 115b: Blade part 119:Second locking protrusion 121,122,123,124,125: 1st discharge hole 126,127,129:Second discharge hole 128: Air exhaust hole 130: Insertion space 131: Stopper 200: Inner chamber 201,202,203,204,205:Second space 210: Upper inner chamber 220: Lower inner chamber 230: Fixed part 300: Cover 301: Cover body 302: Lid 307: First insertion hole 308: Second insertion hole 311, 312, 313, 314, 315: Second protruding member 316, 317: Third protruding member 320: Separation member 400: Base plate 400a: Piston drive part insertion hole 401, 402, 403, 404, 405, 406, 407, 408: Liquid flow path 409: Air flow path 410: Flow cover 410a: 1st through hole 410b: 1st engagement protrusion 410c: Melt protrusion 410d: Second engagement protrusion 411a, 412a, 413a, 414a, 415a, 416a, 417a, 418a: First flow cover hole 411b, 412b, 413b, 414b, 415b: Second flow cover hole 416b, 417b, 418b: 3rd flow cover hole 419a, 419b: 4th flow cover hole 420: Pad 420a: 2nd through hole 421a, 422a, 423a, 424a, 425a, 426a, 427a, 428a: First pad hole 421b, 422b, 423b, 424b, 425b: Second pad hole 426b, 427b, 428b: 3rd pad hole 429a, 429b: 4th pad hole 420c: Binding groove 500: Safety clip 510: Outer chamber joint 520: Handle 530: Upper extension 540: Side extension 541: Cover support member 542: Inner chamber joint 600: Amplification module 610: Body 611: One side 612: Opposite side 613: First end 614:Second end 621:Inlet 622: Outlet 630: Storage unit 631: Upper 632: Lower 633: Bubble storage section 640: Gas transfer passage 641: First gas transfer passage 642: Second gas transfer passage 643: Third gas transfer passage 650:Extract liquid transfer passage 660: First Branch Space 661: First penetration 662: First depression 670: Second Branch Space 671: Second penetration 672: Second depression 700: Piston 701: Fluid storage section 710: Upper piston 711: Close contact area 711a: Drive unit mounting part 712: 1st hole 713: 2nd hole 720: Lower piston 721: Torso 722: Shaft 723: Liquid port 724: Filter port 800: Drive unit 900: Bead chamber 910: 1st bead chamber 911: 1st bead holder 912: 1st dehumidification section 913: Discharge passage 914: Lower opening 920: Second bead chamber 921: Second bead holder 922:Second dehumidification section 923: Discharge passage 924: Lower opening 930: Dehumidifying chamber 1000: Genome extraction device

Claims

1. body; an inlet formed in the body for receiving the extract; a plurality of storage sections connected to the inlet to store the incoming extract; a first branch space communicating with the inlet; a plurality of extract transfer passages branching from the branch space and connecting the inlet and the plurality of storage sections to each other; an outlet formed in the body through which gas is discharged; and a plurality of gas transfer passages interconnecting said outlet and said plurality of chambers;

2. The first branch space is a first penetration portion that penetrates the body and is connected to the inlet; and The amplification module according to claim 1 , further comprising: a first recess formed between the first through-hole and the plurality of extracting liquid passages and recessed into one surface of the body.

3. The amplification module according to claim 2 , wherein at least one of the width and depth of the first branch space is wider or deeper than the width and depth of the extract transfer passage.

4. The amplification module according to claim 1 , wherein the volumes of the plurality of extract liquid transfer passages are the same as each other.

5. the first branch space is formed at a first end of the amplification module in a width direction, The amplification module according to claim 1 , wherein the plurality of accommodating portions are formed at a second end portion opposite the first end portion in a width direction of the amplification module.

6. The gas supply system further includes a second branch space formed between the outlet and the plurality of gas transfer passages, The second branch space is a second penetration portion that penetrates the body and is connected to the outlet; and The amplification module according to claim 2 , further comprising: a second recess formed between the second through-hole and the plurality of gas transfer passages and recessed into another surface of the body.

7. The amplification module according to claim 6 , wherein the first recess and the second recess have overlapping portions in a width direction of the body and are recessed into the body.

8. The amplification module according to claim 7, further comprising a sealing member attached to one surface and an opposite surface of the body, the sealing member sealing the plurality of receiving portions, the first branch space, the second branch space, the extract transfer passage, and the gas transfer passage from an external space.

9. The amplification module according to claim 8 , wherein the space between the first recess and the sealing member and the space between the second recess and the sealing member are independent spaces that do not communicate with each other.

10. The amplification module of claim 1 , wherein the gas transfer passage is connected to an upper portion of the receiving portion, and a space that is wider and deeper than other portions is formed at the connection point.

11. The amplification module according to claim 1 , wherein at least one of the width and depth of the gas transfer passage is narrower or lower than the width and depth of the extract transfer passage.

12. The gas transfer passage is a first gas transfer passageway having a first width and a first depth; a second gas transfer passageway having a second width greater than the first width and the first depth; and a third gas transfer passage having a third width greater than the second width and a second depth greater than the first depth.

13. The amplification module according to claim 11 , wherein the gas transfer passage has a number of protruding pillars.

14. 14. The amplification module according to claim 12, wherein all of the gas transfer passages connected to the plurality of storage sections have the same volume.

15. 2. The amplification module of claim 1, wherein one of the plurality of storage units stores probes and primers for amplifying a first target substance, and another storage unit stores probes and primers for amplifying a second target substance different from the first target substance.

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