Information processing apparatus and information processing method

Abstract

To provide an information processing device and an information processing method capable of easily grasping what kind of peeling condition a peeled cell has undergone by storing information on the cell and information on the peeling condition in association with each other.SOLUTION: An information processing device comprising: a cell information acquisition unit configured to acquire information on a cell; a detachment condition acquisition unit configured to acquire information on a detachment condition for detaching the cell adhering to a culture substrate from the culture substrate by applying an external force to the culture substrate; and a storage unit configured to store the information on the cell and the information on the detachment condition in association with each other.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an information processing device and an information processing method used when detaching cells from a culture substrate. [Background technology]

[0002] With the advancement of regenerative medicine, cell culture techniques using culture plates and petri dishes have been developed for medical treatment and research and development. After culturing, cells adhere to the bottom of the container. Therefore, it is necessary to detach and remove the cells from the container in which they were cultured. Cell detachment methods include adding detachment enzymes or chemicals that act on cell membranes, using temperature-responsive polymers, and applying ultrasonic waves to cells to apply vibrational energy to detach them from the container. A common method is to select and combine multiple detachment methods. Patent Document 1 discloses a technology for detaching cells in a petri dish by externally irradiating them with ultrasonic waves. Patent Document 2 discloses a cell detachment device that reciprocates a culture container held in a holder, colliding the holder with a collision target, and detaching sample cells by the resulting impact and vibration. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] International Publication No. 2019 / 111310 [Patent Document 2] Japanese Patent Application Laid-Open No. 2014-113133 Summary of the Invention [Problem to be solved by the invention]

[0004] As the number and types of cells to be detached increase, the number of detachment conditions also increases, making it difficult to understand the detachment conditions that the detached cells have undergone. [Means for solving the problem]

[0005] The information processing device of the present invention has a cell information acquisition unit that acquires information about cells, a detachment condition acquisition unit that acquires information about detachment conditions for detaching the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate, and a memory unit that stores information about the cells and information about the detachment conditions in association with each other.

[0006] The information processing method of the present invention includes a cell information acquisition step of acquiring information about cells, a detachment condition acquisition step of acquiring information about detachment conditions for detaching the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate, and a storage step of storing the information about the cells and the information about the detachment conditions in association with each other. [Effects of the Invention]

[0007] According to the information processing device and information processing method of the present invention, by storing information about cells and information about detachment conditions in association with each other, it is possible to easily understand what detachment conditions the detached cells have experienced. [Brief explanation of the drawings]

[0008] [Figure 1] 1 is a block diagram illustrating an information processing apparatus according to an embodiment of the present invention. [Figure 2] 1 is a block diagram illustrating a cell detachment system according to an embodiment of the present invention. [Figure 3] 1 is a block diagram showing a hardware configuration of an information processing apparatus according to an embodiment of the present invention; [Figure 4] 1 is a flowchart illustrating an information processing method according to an embodiment of the present invention. [Figure 5] 1 is a block diagram showing an example of a cell detachment system according to an embodiment of the present invention. [Figure 6] 10 is a diagram illustrating an operation screen of a GUI according to an embodiment of the present invention. [Figure 7] 10 is an operation screen after the duration of each state of the GUI has been determined according to the embodiment of the present invention. [Figure 8] 10A and 10B are diagrams showing changes over time in the indicator position on the status display bar according to an embodiment of the present invention. [Figure 9] 10A and 10B are diagrams showing changes over time in the indicator position on the status display bar according to an embodiment of the present invention. [Figure 10] 10A and 10B are diagrams showing changes over time in the indicator position on the status display bar according to an embodiment of the present invention. [Figure 11] 10A and 10B are diagrams illustrating changes over time in the indicator position on the status display bar according to an embodiment of the [Figure 12] 10A and 10B are diagrams showing changes over time in the indicator position on the status display bar according to an embodiment of the present invention. [Figure 13] FIG. 2 is a schematic diagram of an operation screen of a GUI according to an embodiment of the present invention. [Figure 14] FIG. 2 is a schematic diagram of an operation screen of a GUI according to an embodiment of the present invention. [Figure 15] 10 is a flowchart of a peeling operation and storage of peeling conditions according to an embodiment of the present invention. [Figure 16] FIG. 4 is a schematic diagram of a vibration condition setting screen according to the embodiment of the present invention. [Figure 17] FIG. 10 is a schematic diagram of a setting screen for shaking conditions according to an embodiment of the present invention. [Figure 18] FIG. 10 is a schematic diagram of a setting screen for hitting conditions according to the embodiment of the present invention. [Figure 19] FIG. 10 is a schematic diagram showing the contents of a peeling condition storage file according to an embodiment of the present invention. [Figure 20] FIG. 10 is a schematic diagram showing the contents of a peeling condition storage file according to an embodiment of the present invention. [Figure 21] FIG. 10 is a schematic diagram of a peeling condition setting screen according to an embodiment of the present invention. [Figure 22] FIG. 10 is a schematic diagram of a peeling condition setting screen according to an embodiment of the present invention. [Figure 23] FIG. 10 is a schematic diagram of a peeling condition setting screen according to an embodiment of the present invention. [Figure 24] FIG. 10 is a schematic diagram of a peeling condition setting screen according to an embodiment of the present invention. [Figure 25] FIG. 10 is a schematic diagram of a peeling condition setting screen according to an embodiment of the present invention. [Figure 26] FIG. 10 is a schematic diagram of a wizard screen when selecting a peeling condition file according to an embodiment of the present invention. [Figure 27] FIG. 10 is a schematic diagram of a wizard screen when selecting a peeling condition file according to an embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0009] Hereinafter, an information processing device and a cell detachment system according to embodiments of the present invention will be described in detail with reference to the drawings. However, the configurations described in the following embodiments are merely examples, and the scope of the present invention is not limited to the configurations described in the embodiments.

[0010] <First embodiment: information processing device> The information processing device 1000 according to this embodiment includes a cell information acquisition unit 1001 that acquires information about cells, and a detachment condition acquisition unit 1002 that acquires information about detachment conditions for detaching cells adhered to the culture substrate from the culture substrate. The information about the detachment conditions is the condition for detaching cells from the culture substrate by applying an external force to the culture substrate. The information processing device 1000 is characterized by including a memory unit 1005 that stores information about cells and information about the detachment conditions in association with each other. By storing information about cells and information about the detachment conditions in this manner in association with each other, it is possible to easily understand the detachment conditions under which the detached cells were subjected.

[0011] The information processing device 1000 according to this embodiment may include a receiving unit 1003 that receives input of peeling conditions and a display control unit 1004 that controls display of the peeling conditions.

[0012] (External force application) In this embodiment, the external force is applied by, for example, beating the culture substrate, shaking the culture substrate, or vibrating the culture substrate. Vibration of the culture substrate may be achieved by generating ultrasonic waves to vibrate the culture substrate in the ultrasonic band.

[0013] (Information on culture conditions) In this embodiment, the information on the detachment conditions includes at least one piece of information selected from the group consisting of information on the intensity of vibration applied to the culture substrate, information on the frequency of vibration applied to the culture substrate, and information on the time for applying vibration to the culture substrate. Here, the information on the intensity of vibration applied to the culture vessel includes information on the voltage applied to the ultrasonic vibrator that generates ultrasonic waves.

[0014] Furthermore, the information on detachment conditions in this embodiment may be information obtained based on information on the detachment results of cells detached from the culture substrate, which includes at least one piece of information selected from the group consisting of information on the detachment rate of cells, information on the viability of the detached cells, information on the proliferation rate of the detached cells, and information on the functionality of the detached cells.

[0015] Furthermore, the information regarding the detachment conditions in this embodiment may be at least any one of the strength of the beating on the culture substrate, the beating frequency, the beating time, the shaking amplitude, the shaking frequency, and the shaking time.

[0016] When a detachment solution is used to detach cells from the culture substrate, information about the detachment solution may be included in the information about the detachment conditions. When an acoustic matching material is provided between an ultrasonic transducer that generates ultrasonic waves and the culture substrate, information about the acoustic matching material may be included in the information about the detachment conditions.

[0017] (Cell information) In this embodiment, the information about the cells includes information about the cell type.

[0018] (Information on culture conditions) The storage unit may store information about cells, information about detachment conditions, and information about cell culture conditions in association with each other, wherein the information about culture conditions includes at least one piece of information selected from the group consisting of information about cell seeding density, information about the culture medium, information about the culture period, and information about the culture environment.

[0019] (Display control unit) In this embodiment, a display control unit may be provided that controls the display unit to display the operating status of the peeling device. This operating status may include information regarding changes in the operating status of the peeling device over time. The display control unit according to this embodiment may also control the display unit to display the operating conditions of the peeling device, and may display the operating status and the operating conditions in association with each other on the display unit.

[0020] (Cell detachment system) The cell detachment system 1100 according to this embodiment includes the information processing device 1000 according to the embodiment described above and a detachment device 1011 that detaches cells adhered to a culture substrate from the culture substrate by applying an external force to the culture substrate. The detachment device 1011 includes a beating unit that strikes the culture substrate, a shaking unit that shakes the culture substrate, and a vibration generating unit that applies vibrations to the culture substrate. When the vibration generating unit generates vibrations in the ultrasonic band, the vibration generating unit includes an ultrasonic vibrator.

[0021] Furthermore, the cell detachment system 1100 according to this embodiment may include the information processing device 1000 according to this embodiment described above, and a measuring device 1010 that performs measurements to acquire information about cells. The measuring device is not particularly limited as long as it can acquire information about cells, and may include an imaging device that can acquire images of cells or moving images of cells, or an analysis unit that analyzes images of cells or moving images of cells acquired using the imaging device to acquire information about cells.

[0022] (Hardware configuration of information processing device) 3 is a block diagram showing the hardware configuration of an information processing device 1000 according to this embodiment. The information processing device 1000 has a CPU (Central Processing Unit) 1121, a RAM (Random Access Memory) 1122, a ROM (Read Only Memory) 1123, an HDD (Hard Disk Drive) 1124, a communication I / F 1125, a display device 1126, and an input device 1127. These components are connected to each other via a bus or the like.

[0023] The CPU 1121 is a processor that reads out programs stored in the ROM 1123 and the HDD 1124 into the RAM 1122 and executes them, and performs calculation processing and controls each part of the information processing device 1000. The processing performed by the CPU 1121 includes obtaining information about cells, obtaining information about detachment conditions, and obtaining information about culture conditions.

[0024] The RAM 1122 is a volatile storage medium and functions as a work memory when the CPU 1121 executes a program. The ROM 1123 is a non-volatile storage medium and stores firmware and the like required for the operation of the information processing device 1000. The HDD 1124 is a non-volatile storage medium and stores information related to cells, information related to detachment conditions, and information related to culture conditions in association with each other.

[0025] The communication I / F 1125 is a communication device based on standards such as Wi-Fi (registered trademark), Ethernet (registered trademark), Bluetooth (registered trademark), etc. The communication I / F 1125 is used for communication with a measurement device and a peeling device (to be described later), other computers, etc.

[0026] The input device 1127 is a device for inputting information to the information processing device 1000, and is typically a user interface for a user to operate the information processing device 1000. Examples of the input device 1127 include a keyboard, a button, a mouse, a touch panel, and the like.

[0027] The display device 1126 is a device that the information processing device 1000 uses to output information to the outside, and is typically a user interface for presenting information to a user. Examples of the display device 1126 include a display and a speaker.

[0028] The above-described configuration of the information processing device 1000 is an example and can be modified as appropriate. For example, examples of processors that can be installed in the information processing device 1000 include a GPU (Graphics Processing Unit), an ASIC (Application Specific Integrated Circuit), and an FPGA (Field Programmable Gate Array) in addition to the above-described CPU 1121. A plurality of these processors may be provided, and the plurality of processors may perform processing in a distributed manner. The function of storing information such as image data in the HDD 1124 may be provided in another data server rather than within the information processing device 1000. The HDD 1124 may also be a storage medium such as an optical disk, a magneto-optical disk, or an SSD (Solid State Drive).

[0029] The CPU 1121 executes a program to perform predetermined arithmetic processing. The CPU 1121 also executes a program to control each unit in the information processing device 1000. Through these processes, the CPU 1121 realizes the functions of the cell information acquisition unit 1001 and the detachment condition acquisition unit 1002.

[0030] 3 is an example, and other devices may be added, or some devices may not be provided, as long as the functions of the information processing device 1000 according to this embodiment are realized. Also, some devices may be replaced with other devices having similar functions. Furthermore, some functions may be provided by other devices via a network, and the functions constituting this embodiment may be distributed and realized among multiple devices. For example, the HDD 1124 may be replaced with an SSD (Solid State Drive) using semiconductor elements such as flash memory.

[0031] (cell) The cells in this embodiment are not particularly limited as long as they can be cultured in vitro on a culture vessel. For example, various cultured cell lines such as Chinese hamster ovary-derived CHO cells, mouse connective tissue L929 cells, mouse skeletal muscle myoblasts (C2C12 cells), human fetal lung-derived normal diploid fibroblasts (TIG-3 cells), human fetal kidney-derived cells (HEK293 cells), human alveolar basal epithelial adenocarcinoma-derived A549 cells, mouse macrophage-like cells (RAW264.7), and human cervical cancer-derived HeLa cells can be used. In addition, for example, epithelial cells and endothelial cells that constitute various tissues and organs in the body, contractile cells, and the like can be used. Examples of cells that can differentiate include skeletal muscle cells, smooth muscle cells, cardiac muscle cells, neuron cells that make up the nervous system, glial cells, and fibroblasts, hepatic parenchymal cells that are involved in the metabolism of the living body, non-parenchymal liver cells, and adipocytes, as well as various stem cells such as induced pluripotent stem (iPS) cells, embryonic stem (ES) cells, embryonic germ (EG) cells, embryonic carcinoma (EC) cells, mesenchymal stem cells, liver stem cells, pancreatic stem cells, skin stem cells, muscle stem cells, and germline stem cells, as well as progenitor cells of various tissues, and cells induced to differentiate from these.

[0032] The cells in this embodiment may be individual cells (so-called single cells) or sheet-like cell cultures (cell sheets). The cell detachment method according to this embodiment is suitable for cells with strong intercellular bonds, cells with high adhesive strength to a substrate, and cells with high trypsin sensitivity. Given the need for mass cell culture, this method is particularly suitable for, for example, CHO cells used for protein production and mesenchymal stem cells that can be used in cell therapy.

[0033] (Culture substrate) The substrate in this embodiment refers to a culture substrate used for cell culture. The culture substrate is not particularly limited as long as it is a cell-adherent culture substrate, and examples thereof include flasks, tissue culture flasks, dishes, Petri dishes, tissue culture dishes, multi-dishes, microplates, multi-well plates, multi-plates, Petri dishes, culture bags, bottles, etc.

[0034] The material of the culture substrate in this embodiment may be any material that is chemically stable and capable of culturing the desired cells, and examples thereof include polyethylene, polypropylene, polycarbonate, polystyrene, polyvinyl chloride, nylon, polyurethane, polyurea, polylactic acid, polyglycolic acid, polyvinyl alcohol, polyvinyl acetate, poly(meth)acrylic acid, poly(meth)acrylic acid derivatives, polyacrylonitrile, poly(meth)acrylamide, poly(meth)acrylamide derivatives, polysulfone, cellulose, cellulose derivatives, polysilicone, polymethylpentene, glass, metal, etc. Among these, polystyrene is preferred.

[0035] <Second embodiment: information processing method> The information processing method according to this embodiment includes at least the following steps. (1) A cell information acquisition step (S1001) of acquiring information about a cell. (2) A detachment condition acquisition step (S1002) of acquiring information about the detachment conditions for detaching cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate. (3) A storage step (S1003) of storing information relating to the cells and information relating to the detachment conditions in association with each other.

[0036] The information processing method according to this embodiment may acquire information about cell culture conditions. In this case, in step (3), the information about the cells, the information about the detachment conditions, and the information about the cell culture conditions may be stored in association with each other.

[0037] <Third embodiment: Graphical user interface (GUI)> When detaching cells using multiple detachment means as in Patent Document 2, the setting, loading, and saving of the detachment conditions require a different approach than a GUI for a user to control a detachment means using a single detachment means. For example, the following graphical user interface is conceivable.

[0038] The operating and non-operating states of a detachment means (detachment device) operating in multiple modes over time may be displayed in association with the operating conditions entered by the user on a graphical user interface. The operating and non-operating states of the modes may also be displayed as a bar graph arranged in chronological order with lengths proportional to their duration. This bar graph may be displayed vertically, horizontally, or concentrically on a common time axis on the graphical user interface, and the shape of the bar graph may change according to values ​​entered by the user on the graphical user interface. The graphical user interface may also highlight a portion of the bar graph corresponding to a specific mode over time while the device is operating in a specific mode. The bar graph may also have an indicator indicating the current time, and the indicator's position on the bar graph may change over time. The graphical user interface may also have a storage medium for caching or saving the detachment conditions, and the graphical user interface may have a continuation check box. When the detachment operation by the cell detachment device is started with the continuation check box checked, the conditions for the detachment operation may be added to the existing cache. Furthermore, when the detachment operation using the cell detachment device is started with the continuation check box unchecked, the existing cache may be overwritten with the detachment operation conditions. Furthermore, when the detachment conditions are saved at the end of the detachment operation, the cache may be saved as experimental data in a format that can be written and read from a storage medium, and the cache at that time may be erased from the storage medium. Furthermore, if detachment is completed with the check box checked, the cell sheet adhesion prevention operation may be performed until the next detachment operation is started unless the check box is unchecked. The adhesion prevention operation may be a detachment operation that is one of multiple modes or a combination of multiple modes. The cell detachment device may have a storage medium that stores the detachment conditions, and the graphical user interface may have multiple detachment condition editing buttons that write or read the detachment conditions from the storage medium.The cell detachment device may include a storage medium for storing the detachment conditions, and the detachment operation edit button corresponding to the detachment condition selected by the user and that led to the start of the detachment operation may be positioned on the graphical user interface in the order in which the detachment operations were performed. The graphical user interface may have a peeling condition reading means for reading a peeling condition file from a storage medium. When peeling is actually performed using the peeling condition file selected by the peeling condition reading means, an editing action may be performed on the peeling condition file. The editing action may be changing the timestamp of the peeling condition file. The editing action may be adding a timestamp to the prefix of the file name of the peeling condition file. The editing action may also be changing the contents of the peeling condition file to the extent that the settings of the setting file are not changed.

[0039] Below, an example of a GUI suitable for detaching cells using multiple detachment means is shown.

[0040] [An example of a cell detachment system] FIG. 5 is a bird's-eye view of a cell detachment system according to an embodiment of the present invention. The detachment device 1011 detaches cells from a culture substrate by applying an external force to the cells adhered to the culture substrate. The detachment device 1011 converts power supplied from a control unit 1020 into mechanical motion for applying the external force using a DC motor or the like. The external force control unit 1020 generates an electrical signal based on the specified detachment conditions and sends the electrical signal to the detachment device 1011. The reception unit 1003 (not shown) receives the detachment conditions specified by the user via a GUI and sends a signal indicating the specified detachment conditions to the control unit 1020, which controls the start and stop of the detachment operation. The system also includes a memory unit 1005 (shown in the accompanying drawings) that stores the detachment conditions. The memory unit 1005 can also temporarily store the detachment conditions as a cache. The display unit 1030 can display the detachment conditions under the control of the display control unit 1004.

[0041] [GUI example 1] FIG. 6 shows a GUI operation screen according to Example 1. This operation screen is displayed on the display unit 1030. As described above, in this example, the peeling operation has three modes: peeling means 1, peeling means 2, and peeling means 3, each of which has buttons 101, 102, and 103 for setting the peeling conditions. Examples of peeling means are means for executing the method described above in the application of external force. Pressing each button opens a peeling condition setting wizard, allowing the peeling conditions to be set. In this example, the three modes of peeling operation are repeated a specified number of times at a user-specified cycle. The user inputs the number of repetitions into the set number input unit 106. After driving begins, the current number of repetitions is displayed in the current number display unit 105. The three modes each transition between standby 1, driving, standby 2, and interval driving states, and are repeated a set number of times. Driving is a state in which the peeling operation is actually being performed in that mode. Other driving states are states in which the peeling operation is paused. The user can input the duration of each driving state into the state duration input units 111 to 119. 111 to 113 allow input of the duration of each of the standby 1, drive, and standby 2 states in vibration mode. Similarly, 114 to 116 allow input of the duration of each of the modes for the peeling means 2, and 117 to 119 allow input of the duration of each of the modes for the peeling means 3. The duration of the interval state is common to all three modes and is input in the interval time input section 104. The drive cycle is the sum of the times for standby 1, drive, standby 2, and interval. Depending on the values ​​entered by the user, this cycle may not be the same in all three modes. In this case, pressing the confirm button 100 after entering the value automatically corrects the input value for standby 2 by adjusting the standby 2 time so that each cycle in the three modes is the same. 121 to 129 are state transition display bars. These are provided to clearly indicate the operating and non-operating states in the three modes. Only the time region in the drive state within one cycle is displayed as a dot. In Figure 6, the duration of each state in the three modes does not correspond to the display of the state transition display bar.

[0042] FIG. 7 shows an operation screen after the duration of each state of the GUI according to an embodiment of the present invention has been confirmed. After entering the durations and intervals of states 111 to 119 on the operation screen in FIG. 6, the confirm button 100 is pressed. In FIG. 7, the durations of the standby 1 state, drive state, standby 2 state, and interval state are 0.5, 1.0, 0.5, and 0.5 seconds, respectively, for the vibration mode cycle. Therefore, the operation cycle is 0.5 + 1.0 + 0.5 + 0.5 = 2.5 seconds. In the state transition display bar corresponding to the vibration mode, the portion corresponding to the duration of the standby 1 state is displayed as 121, the portion corresponding to the drive state as 122, and the portions corresponding to the standby 2 and interval states as 123. The length of each state transition display bar is displayed in proportion to the duration. The same applies to the modes of peeling means 2 and peeling means 3. As shown in Figure 7, by pressing the Confirm button 100 to confirm the duration, state transition display bars 121-129 are displayed corresponding to the values ​​entered in the state duration input fields 111-119 and interval time input field 104. This allows the user to visually confirm the conditions entered numerically, preventing incorrect input of detachment conditions. In particular, with a cell detachment method using three methods, it is important to determine which modes to use in combination. Arranging the state transition display bars vertically, as shown in Figures 6 and 7, makes it easier to compare the start and end points of the drive states in each mode, which is useful when considering detachment conditions.

[0043] 8 to 12 show the change over time in the indicator position on the status display bar according to this embodiment. When the drive start button 107 in FIG. 7 is pressed to start the peeling operation, the indicator 109 is displayed on the status transition display bar 121 to 129. As time passes, the indicator 109 moves from left to right on the status transition display bar. FIG. 8 shows the position of the indicator 109 immediately after the drive start button 107 is pressed, which is at the leftmost position on the status transition display bar. As time passes, the indicator 109 for each mode moves rightward on the status transition display bar for the standby time 1. FIG. 9 shows the position of the indicator 0.75 seconds after the start of the peeling operation. At this point, the device is in the operating state in the vibration mode and the beating mode, so the vibration condition setting button 101 and the beating condition setting button 103 are highlighted. FIG. 10 shows the position of the indicator 1.25 seconds after the start of the peeling operation. At this point, all modes are in the driven state, so the vibration condition setting button 101, shaking condition setting button 102, and beating condition setting button 103 are highlighted. Figure 11 shows the position of the indicator 1.75 seconds after the start of the peeling operation. At this point, the shaking and beating modes are in the driven state, so the condition setting button 102 for peeling means 2 and the condition setting button 103 for peeling means 3 are highlighted. Figure 12 shows the position of the indicator 2.25 seconds after the start of the peeling operation. At this point, all modes are in the de-driven state, so no condition setting button is highlighted.

[0044] As described above, the indicator 109 moves on the state transition display bars 121 to 129 over time, and when the indicator 109 is positioned on the drive state of each mode, the condition setting button for each mode is highlighted, making it easy to understand the current drive / non-drive state.

[0045] [GUI example 2] The configuration of the cell detachment system in Example 2 is the same as that in Example 1. Only the configuration of the GUI display screen on the display unit 1030 differs from that in Example 1. Figure 13 shows the GUI operation screen according to Example 2. Unlike the GUI operation screen of Example 1 in Figure 6, the state transition display bar is displayed vertically. As with Example 1, this method also allows the user to visually and easily grasp the driving and non-driving states of each mode.

[0046] [GUI example 3] The configuration of the cell detachment system in Example 3 is the same as that in Example 1. Only the configuration of the GUI display screen on the display unit 1030 differs from that in Example 1. Figure 14 shows the GUI operation screen according to Example 3. Unlike the GUI operation screen of Example 1 in Figure 6, a state transition display bar is displayed in concentric circles. As with Example 1, this method also allows the user to visually and easily grasp the drive and non-drive states of each mode.

[0047] [GUI example 4] The configuration of the cell detachment system in Example 4 is the same as that in Example 1. The configuration of the GUI display screen on the display unit 1030 is also the same as that in Example 1, as shown in FIG. 6. In FIG. 6, the detachment conditions can be saved by pressing the detachment condition write button 145. In Example 4, the saving sequence of these detachment conditions varies depending on whether the continue button 108 is checked or not. FIG. 15 is a flowchart of saving detachment conditions according to Example 4. After inputting the detachment conditions, the detachment operation is started by pressing the drive start button 100 (S102). If the continue button 108 is checked, the cache of information on the detachment conditions used in the previous detachment operation is maintained, and the information on the detachment conditions set for this detachment operation is added to the cache (S103, S104). If the continue button 108 is not checked, the cache of information on the detachment conditions used in the previous detachment operation is erased, and the information on the detachment conditions set for this detachment operation is saved as a cache (S103, S104, S105). The detachment operation then ends (S106). If the peeling conditions are saved by pressing the peeling condition write button 145, the cache in the storage device is saved as a file as the peeling conditions (S107, S108). After saving the file, the cache is deleted (S109). If the peeling conditions are not saved and the process proceeds to the next peeling operation, the process proceeds to the sequence of S102.

[0048] FIG. 16 shows a vibration condition setting screen for Example 4. This screen can be displayed by pressing the vibration condition setting button 101. The vibration mode inputs a sine wave voltage signal of a specified amplitude over a specified frequency range for a specified sweep time to the ultrasonic wave generator 303. Therefore, the vibration peeling condition input section allows the user to specify the sweep time, maximum frequency, minimum frequency, and (sine wave) amplitude. The peeling pattern selection section 133 allows the user to select the following sweep patterns: (i) a SWEEP UP pattern in which the sweep frequency sweeps from the lowest frequency to the highest frequency; (ii) a SWEEP DOWN pattern in which the sweep frequency sweeps from the highest frequency to the lowest frequency; (iii) a SWEEP UP-DOWN pattern in which the frequency swings back and forth between the lowest and highest frequencies within the sweep time; and (iv) a BURST pattern in which the frequency remains constant.

[0049] 17 is a screen for setting the conditions of the peeling means 2 according to Example 4. When the peeling means 2 is a vibration type, it is possible to specify a target frequency for setting the frequency of the reciprocating motion of the peeling means 2 and PID coefficients (Kp, Ki, Kd) for controlling the linear motor that operates the peeling means 2.

[0050] 18 is a screen for setting the conditions of the detachment means 3 according to Example 4. When the detachment means 3 is beating, the frequency with which the culture vessel 305 is beated can be designated.

[0051] 16 to 18. For example, the impact force applied to the culture vessel 305 during beating can be specified as a percussion condition. Other specifiable experimental conditions include the date of the experiment, location, operator's name, cell type, lot number, passage number, number of days in culture, number of cells, number of seeds, type of medium, volume of culture medium, additives to the culture medium, method of cleaning the culture vessel, type of cleaning solution, volume of cleaning solution, number of washes, type of detachment solution for detaching the cell sheet, type and volume of detachment solution (e.g., EDTA, PBS, trypsin), survival rate after detachment, type of culture vessel, type of vibration transmitter, and temperature, humidity, and CO2 activity as part of the experimental environment.

[0052] FIG. 19 is a diagram showing the contents of a peeling condition file according to Example 4. This file was created when three peeling operations were performed with the continue button 108 pressed, and the peeling conditions were not saved for the first and second peeling operations. It can be seen that the peeling conditions for the first through third operations are arranged in chronological order and saved as a single file. Each peeling operation is assigned a separate line for each of the three methods. The conditions are written in the following order: lot number, date and time, peeling method, and in the case of vibration mode, pattern, number of repetitions, interval, duration of standby 1 state, duration of drive state, duration of standby 2 state, followed by the peeling conditions specific to each method.

[0053] 20 is a diagram showing the contents of the peeling condition saved file for Example 4. This file is created when the peeling operation is performed twice without pressing the continue button 108, and the peeling conditions for the first peeling are not saved. The peeling conditions for the first peeling are not recorded in the saved file, and only the peeling conditions for the second peeling are saved.

[0054] By providing the continue button 108 in this way, it is possible to save the experimental conditions collectively as one file after completing any number of peeling operations, without having to save the experimental conditions each time.

[0055] [GUI example 5] 21 to 24 show detachment condition setting screens related to Example 5. The configuration of the cell detachment system in Example 5 is the same as in Example 1. Only the configuration of the GUI display screen on the display unit 1030 differs from Example 1. In this example, four detachment condition edit buttons 141 are arranged as shown in FIG. 21. Pressing each button opens a screen for editing experimental conditions, as in Example 4. The detachment condition edit buttons 141 are arranged from left to right in chronological order, in the order in which the detachment operation was actually performed under the detachment conditions. For example, in the display state of FIG. 21, the order of the detachment conditions used is D → C → B → A. Pressing the detachment condition selection tab 142 here displays the available detachment conditions as shown in FIG. 22, from which one can be selected. FIG. 23 shows the state in which condition C is selected. If the drive start button 107 is pressed here to start the detachment operation, the most recently used detachment conditions will be conditions C, A, B, and C in chronological order. Therefore, the detachment condition edit buttons are arranged as shown in FIG. 24. This allows the user to easily recall the peeling conditions used in chronological order, allowing for GUI operation with excellent condition setting convenience.

[0056] [GUI Examples 6 and 7] FIG. 25 shows a detachment condition setting screen for Examples 6 and 7. The configuration of the cell detachment system in Examples 6 and 7 is the same as in Example 1. Only the configuration of the GUI display screen on the display unit 1030 differs from Example 1. The GUI operation screen includes a detachment condition load button 144, which, when pressed, calls up a screen for selecting a detachment condition file stored in a storage device. The selected detachment conditions are displayed in the detachment condition display unit 143. Pressing the drive start button 107 starts the detachment operation using the detachment conditions displayed in the detachment condition display unit 143. FIG. 26 shows a wizard screen for selecting a detachment condition file for Example 6. This wizard screen is launched by pressing the detachment condition load button 144. In Example 6, when the detachment operation actually starts using the selected detachment condition file, the timestamp of the detachment condition file is rewritten to that time. For example, assume that the name and timestamp of the detachment condition file in the detachment condition selection wizard before detachment begins are as shown in the left screen of FIG. 26. In this case, if you select the file with condition D as the peeling condition and start the peeling operation, the timestamp of the peeling condition file with condition D will be rewritten to 2024 / 03 / 12, the date and time when peeling started. By doing this, you can sort the peeling condition files in order of update date and time (timestamp) on the peeling condition selection wizard screen shown on the right side of Figure 26. In other words, you can sort the peeling condition files on the wizard screen in order of most recent time of use.

[0057] Figure 27 shows the wizard screen for selecting a stripping condition file in Example 7. In Example 7, when the stripping operation actually starts based on the selected stripping condition file, the timestamp of the stripping condition file is written to the prefix of its file name. For example, assume that the name and timestamp of the stripping condition file in the stripping condition selection wizard before stripping starts are as shown in the left screen of Figure 27. In this case, if you select the file with condition D as the stripping condition and start the stripping operation, the prefix of the stripping condition file for condition D is rewritten from 240312092219 to 240420085122, the date and time when stripping started. In this way, by sorting the stripping condition files by name on the stripping condition selection wizard screen on the right screen of Figure 27, it is possible to sort the stripping condition files in chronological order of the time when the stripping conditions were actually used.

[0058] As described above, the GUI described in this embodiment provides users with high operability when setting, loading, and saving detachment conditions to detach cells from a culture vessel using multiple modes, as well as when performing actual detachment operations under those detachment conditions. Specifically, when detaching a cell sheet from a culture vessel using multiple modes, the detachment condition settings can be visualized and checked at any time. Furthermore, when loading detachment conditions, the detachment conditions can be recalled in the order in which the detachment operations were performed, enhancing user convenience. Furthermore, when saving detachment conditions, the experimental conditions for multiple detachment operations leading up to detachment can be compiled into a single detachment condition file, reducing the workload. Overall, this makes it possible to provide a highly user-friendly GUI for users performing cell detachment operations.

[0059] The present invention may be realized, for example, as follows: First, an information processing program that realizes one or more functions of the above-described embodiments is supplied to a system or device via a network or a storage medium. Then, one or more processors in a computer of the system or device read and execute the information processing program. Furthermore, the present invention may be realized, for example, by a circuit (e.g., an ASIC) that realizes one or more functions.

[0060] Although preferred embodiments of the present invention have been described above, they are merely examples of specific embodiments for carrying out the present invention, and the technical scope of the present invention should not be construed as being limited by these. In other words, the present invention can be embodied in various forms without departing from its technical concept or main features. For example, it should be understood that embodiments in which part of the configuration of any embodiment is added to or substituted for part of the configuration of another embodiment are also embodiments to which the present invention can be applied.

[0061] Embodiments of the present disclosure include the following methods and compositions. (Configuration 1) a cell information acquisition unit that acquires information about cells; a detachment condition acquisition unit that acquires information about detachment conditions for detaching the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate; an information processing device having a memory unit that stores information about the cells and information about the detachment conditions in association with each other. (Configuration 2) 2. The information processing device according to configuration 1, wherein the external force is applied to the culture substrate by applying vibration to the culture substrate. (Configuration 3) The information processing device according to configuration 2, wherein the information regarding the peeling conditions includes at least one piece of information selected from the group consisting of information regarding the intensity of vibration applied to the culture substrate, information regarding the frequency of vibration applied to the culture substrate, and information regarding the time for applying vibration to the culture substrate. (Configuration 4) The vibration applied to the culture vessel is vibration caused by applying ultrasonic waves generated by an ultrasonic vibrator, 3. The information processing device according to configuration 2, wherein the information regarding the intensity of the vibration to be applied to the culture vessel includes information regarding the voltage to be applied to the ultrasonic vibrator. (Configuration 5) An information processing device according to any one of configurations 1 to 4, wherein the information regarding the detachment conditions includes at least one piece of information selected from the group consisting of information regarding a detachment solution used to detach the cells from the culture substrate and information regarding an acoustic matching material. (Configuration 6) the information on the detachment conditions is information obtained based on information on the detachment results obtained by detaching the cells from the culture substrate, An information processing device described in any one of configurations 1 to 5, wherein the information regarding the detachment result includes at least one piece of information selected from the group consisting of information regarding the detachment rate of cells, information regarding the survival rate of the detached cells, information regarding the proliferation rate of the detached cells, and information regarding the functionality of the detached cells. (Configuration 7) 7. The information processing device according to any one of configurations 1 to 6, wherein the information about the cells includes information about cell types. (Configuration 8) 8. The information processing device according to any one of configurations 1 to 7, wherein the storage unit stores information about the cells, information about the detachment conditions, and information about the culture conditions of the cells in association with each other. (Configuration 9) 9. The information processing device according to claim 8, wherein the information regarding the culture conditions further includes at least one piece of information selected from the group consisting of information regarding the cell seeding density, information regarding the culture medium, information regarding the culture period, and information regarding the culture environment. (Configuration 10) The information processing device according to any one of configurations 1 to 9, a detachment device that detaches the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate. (Configuration 11) a measuring device that performs measurements to obtain information about the cells; The information processing device according to any one of configurations 1 to 10, A cell detachment system having (Configuration 12) An information processing device according to any one of configurations 1 to 9, comprising a display control unit that controls the display unit to display the operating status of a detachment device that detaches the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate. (Configuration 13) 13. The information processing device according to configuration 12, wherein the driving state includes information about a change over time in the driving state of the peeling device. (Configuration 14) 14. The information processing device according to configuration 12 or 13, wherein the display control unit controls a display unit to display the operating conditions of the peeling device. (Configuration 15) 13. The information processing device according to configuration 12, wherein the display control unit controls the display unit to display the driving state and the driving conditions of the peeling device in association with each other. (Method 1) a cell information acquisition step of acquiring information about the cell; a detachment condition acquisition step of acquiring information about detachment conditions for detaching the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate; and a storage step of storing the information about the cells and the information about the detachment conditions in association with each other. [Explanation of symbols]

[0062] 1000 Information Processing Device 1001 Cell information acquisition department 1002 Peeling condition acquisition unit 1003 Reception 1004 Display control unit 1005 Storage section

Claims

1. a cell information acquisition unit that acquires information about cells; a detachment condition acquisition unit that acquires information about detachment conditions for detaching the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate; an information processing device having a memory unit that stores information about the cells and information about the detachment conditions in association with each other.

2. The information processing device according to claim 1 , wherein the external force is applied to the culture substrate by applying vibration to the culture substrate.

3. 3. The information processing device of claim 2, wherein the information regarding the peeling conditions includes at least one piece of information selected from the group consisting of information regarding the intensity of the vibration applied to the culture substrate, information regarding the frequency of the vibration applied to the culture substrate, and information regarding the time for which vibration is applied to the culture substrate.

4. The vibration applied to the culture vessel is vibration caused by applying ultrasonic waves generated by an ultrasonic vibrator, The information processing device according to claim 2 , wherein the information regarding the intensity of the vibration applied to the culture vessel includes information regarding a voltage applied to the ultrasonic vibrator.

5. The information processing device according to claim 1 , wherein the information regarding the detachment conditions includes at least one piece of information selected from the group consisting of information regarding a detachment solution used to detach the cells from the culture substrate and information regarding an acoustic matching material.

6. the information on the detachment conditions is information obtained based on information on the detachment results obtained by detaching the cells from the culture substrate, 2. The information processing device of claim 1, wherein the information regarding the detachment result includes at least one piece of information selected from the group consisting of information regarding the detachment rate of cells, information regarding the survival rate of the detached cells, information regarding the proliferation rate of the detached cells, and information regarding the functionality of the detached cells.

7. The information processing device according to claim 1 , wherein the information about the cells includes information about a cell type.

8. The information processing device according to claim 1 , wherein the storage unit stores the information on the cells, the information on the detachment conditions, and the information on the culture conditions of the cells in association with each other.

9. The information processing device according to claim 8 , wherein the information on the culture conditions further includes at least one piece of information selected from the group consisting of information on cell seeding density, information on the culture medium, information on the culture period, and information on the culture environment.

10. An information processing device according to any one of claims 1 to 9; a detachment device that detaches the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate.

11. a measuring device that performs measurements to obtain information about the cells; An information processing device according to any one of claims 1 to 9; A cell detachment system having

12. 10. The information processing device according to claim 1, further comprising a display control unit that controls the display unit to display the operating status of a detachment device that detaches the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate.

13. The information processing apparatus according to claim 12 , wherein the driving state includes information about a change over time in the driving state of the peeling device.

14. The information processing apparatus according to claim 12 , wherein the display control unit controls a display unit to display the operating conditions of the peeling device.

15. The information processing apparatus according to claim 12 , wherein the display control unit controls the display unit to display the driving state and the driving conditions of the peeling device in association with each other.

16. a cell information acquisition step of acquiring information about the cell; a detachment condition acquisition step of acquiring information about detachment conditions for detaching the cells adhered to the culture substrate from the culture substrate by applying an external force to the culture substrate; and a storage step of storing the information about the cells and the information about the detachment conditions in association with each other.

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