Oral composition

Abstract

The object of this disclosure is to provide an oral composition containing Rhodiola rosea extract with enhanced antioxidant activity. [Solution] By incorporating lutein and / or zeaxanthin into an oral composition containing Rhodiola rosea extract, the antioxidant effect is improved.

Description

Technical Field

[0001] The present disclosure relates to an oral composition containing rhaponticum extract.

Background Art

[0002] Rhaponticum extract contains various polyphenols and is known to have antioxidant activity (Non-Patent Document 1). Furthermore, rhaponticum extract is known to have anxiolytic effects, effects of improving falling asleep and maintaining sleep, stress-reducing effects, relaxation-improving effects, performance or concentration-improving effects, effects of improving the effect of rest, and fatigue-recovery promoting effects, and is known to be orally administrable (Patent Document 1).

Prior Art Documents

Non-Patent Documents

[0003]

Non-Patent Document 1

Patent Documents

[0004]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0005] In order to enhance the effectiveness of an oral composition containing rhaponticum extract, a formulation that further enhances its expected antioxidant action is desired. However, such formulations have not been sufficiently studied.

[0006] Therefore, the present disclosure aims to provide an oral composition containing Rhodiola rosea extract with improved antioxidant activity. [Means for solving the problem]

[0007] The inventors of the present invention conducted diligent research to solve the aforementioned problems and found that the antioxidant effect is improved by adding lutein and / or zeaxanthin to an oral composition containing Rhodiola rosea extract. This disclosure was completed by further research based on this finding.

[0008] In other words, this disclosure provides inventions in the following embodiments. Item 1. An oral composition comprising (A) Rhodiola rosea extract and (B) lutein and / or zeaxanthin. Item 2. The oral composition according to Item 1, wherein the total amount of component (B) is 0.001 to 20 parts by weight per 1 part by weight of component (A). Item 3. An oral composition according to item 1 or 2, used as an antioxidant. [Effects of the Invention]

[0009] According to this disclosure, an oral composition containing Rhodiola rosea extract with enhanced antioxidant activity is provided. [Modes for carrying out the invention]

[0010] The oral composition of this disclosure is characterized by containing (A) Rhodiola rosea extract (hereinafter also referred to as "component (A)") and (B) lutein and / or zeaxanthin (hereinafter also referred to as "component (B)"). The oral composition of this disclosure will be described in detail below. In this disclosure, the numerical range "X~Y" refers to a range of X or more and Y or less.

[0011] (A) Rhodiola extract The oral composition of the present disclosure contains a raffia extract as component (A). The raffia extract has an antioxidant effect, but the oral composition of the present disclosure exhibits an antioxidant effect at a level exceeding the antioxidant effect corresponding to the blending amount of the raffia extract.

[0012] Raffia (Apocynum venetum L.) means a plant belonging to the family Apocynaceae. Examples of the part of raffia (extraction target part) used for the preparation of the raffia extract include the whole plant, aerial part, leaves, stems, etc. As the extraction target part, one of these extraction target parts may be used alone, or two or more of them may be used in combination. Among these extraction target parts, from the viewpoint of antioxidant action, the leaves of raffia are preferably mentioned.

[0013] Regarding the extraction treatment for obtaining the raffia extract, any general extraction method used for the production of plant extracts may be used. Examples include solvent extraction treatment, supercritical extraction treatment, steam distillation treatment, etc. Among these, solvent extraction treatment is preferably mentioned.

[0014] Examples of the extraction solvent used in the solvent extraction treatment include water; monohydric alcohols having 1 to 6 carbon atoms such as methanol and ethanol; polyhydric alcohols such as propylene glycol and 1,3 - butylene glycol; other polar solvents such as acetone and ethyl acetate; and mixed solvents thereof. Among these extraction solvents, water, monohydric alcohol, and mixed solvents thereof are preferably mentioned, more preferably water, ethanol, and aqueous ethanol, and even more preferably aqueous ethanol.

[0015] The solvent extraction treatment can be carried out by immersing the part of raffia to be extracted, which has been subjected to treatments such as drying, cutting, and pulverization as necessary to enhance the extraction efficiency, in the extraction solvent and stirring as necessary. For example, the solvent extraction treatment can be carried out by immersing it in an extraction solvent in an amount 2 to 100 times the dry weight of the part of raffia to be extracted and carrying out the treatment at, for example, 60°C or higher, preferably at the heating reflux temperature, for about 0.5 to 24 hours.

[0016] After the extraction process, by removing solids through solid-liquid separation, a rough extract is obtained in the form of an extract solution. The obtained extract solution may, if necessary, be subjected to a filtration process; various adsorption treatments such as various chromatographies using a column filled with a carrier such as polystyrene gel (polystyrene-divinylbenzene copolymer, etc.), ion exchange resin, activated carbon, etc., and then subjected to a purification process. Alternatively, the obtained extract solution may be used as it is as a non-concentrated extract, or may be subjected to a concentration process as necessary and used as a soft extract, or may further be subjected to a drying process and used as an extract powder.

[0017] The rough extract contains hyperoside and isoquercitrin, which are flavonoid compounds. Examples of the content of hyperoside per 100 parts by weight of the dry weight of the rough extract include, for example, 1 to 10 parts by weight, preferably 1.5 to 10 parts by weight, or 1.5 to 5 parts by weight. Examples of the content of isoquercitrin per 100 parts by weight of the dry weight of the rough extract include, for example, 1 to 10 parts by weight, preferably 1.5 to 10 parts by weight, or 1.5 to 5 parts by weight.

[0018] The content of the component (A) in the oral composition of the present disclosure may be appropriately set according to the degree of antioxidant effect required, but in terms of dry weight, for example, it is 1 to 20% by weight, preferably 5 to 15% by weight.

[0019] (B) Lutein and / or zeaxanthin The oral composition of the present disclosure contains lutein and / or zeaxanthin as the component (B). Both lutein and zeaxanthin improve the antioxidant effect by being combined with the component (A) in the oral composition of the present disclosure.

[0020] Lutein and zeaxanthin are included in xanthophyll, which is a type of carotenoid. Both lutein and zeaxanthin have the chemical formula C 40 H 56 O2 and are structural isomers that differ only in the position of the double bond in one terminal ring.

[0021] In the oral composition of the present disclosure, a product of lutein and / or zeaxanthin may be used, or an extract of a plant containing at least one of them may be used.

[0022] The plants containing lutein and / or zeaxanthin are not particularly limited. For example, as plants containing lutein, there are dark green leafy vegetables such as kale, collard greens, spinach, etc., marigold (Tagetes erecta), wolfberry (Lycium barbarum), potato, large red algae, etc. As plants containing zeaxanthin, there are paprika, corn, persimmon, marigold (Tagetes erecta), wolfberry (Lycium barbarum), specific potatoes (Inca's eye, Nagasaki gold (registered trademark), and Inca's awakening), red algae, etc. These plants may be used alone or in combination of two or more. Among these plants, preferably marigold (extraction target part: petal), wolfberry (extraction target part: fruit) are mentioned, and more preferably marigold (extraction target part: petal) is mentioned.

[0023] Regarding the extraction treatment for obtaining the extract of the above plants, any general extraction method used in the production of plant extracts may be used, for example, solvent extraction treatment, supercritical extraction treatment, steam distillation treatment, etc. Among these, preferably solvent extraction treatment is mentioned.

[0024] Examples of the extraction solvent used in the solvent extraction treatment include water; monohydric alcohols having 1 to 6 carbon atoms such as methanol and ethanol; polyhydric alcohols such as propylene glycol and 1,3 - butylene glycol; other polar solvents such as acetone and ethyl acetate; non - polar solvents such as hexane; and mixed solvents thereof. Among these extraction solvents, preferably non - polar solvents are mentioned, and more preferably hexane is mentioned.

[0025] Solvent extraction can be carried out by immersing the target material, which has been subjected to drying, cutting, grinding, or other treatments as necessary to improve extraction efficiency, in an extraction solvent and stirring as needed. For example, solvent extraction can be carried out by immersing the target material in an extraction solvent in an amount of 2 to 100 times its dry weight, at a temperature of, for example, 60°C or higher, preferably a reflux temperature, for about 0.5 to 24 hours.

[0026] After the extraction process, the plant extract is obtained in the form of an extract by removing solid matter through solid-liquid separation. The obtained extract may be purified by filtration, adsorption treatment using various chromatography methods such as polystyrene gel (polystyrene-divinylbenzene copolymer, etc.), ion exchange resin, or activated carbon columns, as needed. The obtained extract may be used as is as a non-concentrated extract, or it may be concentrated as a soft extract as needed, or it may be further dried as an extract powder.

[0027] In the oral compositions of this disclosure, from the viewpoint of antioxidant activity, it is preferable to use a marigold extract in the formulation of component (B). In other words, it is preferable that the oral compositions of this disclosure contain a marigold extract. The lutein and zeaxanthin content per 100 parts by weight of dry weight of the marigold extract is, for example, 30 to 80 parts by weight, preferably 50 to 75 parts by weight, for lutein and 5 to 40 parts by weight, preferably 10 to 20 parts by weight, for zeaxanthin.

[0028] In the oral composition of this disclosure, the ratio of component (A) to component (B) is not particularly limited and may be set appropriately according to the desired degree of antioxidant effect. For example, the content (total amount) of component (B) per 1 part by weight (dry weight) of component (A) is 0.001 to 20 parts by weight, preferably 0.05 to 5 parts by weight, more preferably 0.08 to 3 parts by weight, even more preferably 0.2 to 2 parts by weight, even more preferably 0.3 to 1 part by weight, and particularly preferably 0.4 to 0.8 parts by weight or 0.4 to 0.6 parts by weight.

[0029] The specific content of component (B) in the oral composition of this disclosure is determined by the content of component (A) and the ratio of component (A) to component (B), but the total amount is preferably 0.5 to 8% by weight, more preferably 1 to 6% by weight or 2 to 5% by weight.

[0030] In the oral compositions of this disclosure, when a plant extract is used in the formulation of component (B), the ratio of component (A) to the plant extract should be determined such that the ratio of component (A) to the content of component (B) contained in the plant extract is the above ratio. For example, the content of plant extract (dry weight) per 1 part by weight (dry weight) of component (A) may be, for example, 0.004 to 80 parts by weight, preferably 0.01 to 20 parts by weight, more preferably 0.05 to 10 parts by weight, even more preferably 0.1 to 5 parts by weight or 0.1 to 1 part by weight, and even more preferably 0.3 to 0.8 parts by weight.

[0031] In the oral composition of this disclosure, when a plant extract is used in the formulation of component (B), the specific content of the plant extract in the oral composition of this disclosure is determined by the content of component (A) and the ratio of component (A) to the plant extract, but preferably 0.5 to 20% by weight, more preferably 1 to 10% by weight or 1 to 7% by weight, based on dry weight.

[0032] Other ingredients The oral compositions of this disclosure may or may not contain other nutritional and / or pharmacological components in addition to the components (A) and (B) described above. Such optional nutritional and / or pharmacological components are not particularly limited as long as they are usable in food or oral medicine, but examples include vitamins, amino acids, minerals, carbohydrates, fatty acids, other plant extracts, other antioxidants, hypoglycemic agents, anticholinergic agents, and immunostimulants. These components may be used individually or in combination of two or more. The content of these components may be appropriately determined depending on the type of additive used or the intended use of the oral composition.

[0033] Furthermore, the oral compositions of this disclosure may or may not contain, in addition to the aforementioned components (A) and (B), a base and / or additives, as necessary, in order to prepare them into a desired formulation. Such bases and / or additives, whether present or absent, are not particularly limited as long as they are usable in food or oral pharmaceuticals, but examples include excipients, disintegrants, lubricants, binders, thickeners, monohydric alcohols with 1 to 5 carbon atoms, higher alcohols with 6 or more carbon atoms, oils and fats, waxes, water-soluble polymers, surfactants, polyhydric alcohols, pH adjusters, buffers, antioxidants, preservatives, chelating agents, and water. These components may be used individually or in combination of two or more. The content of these components is appropriately determined according to the type of base and / or additive used and the dosage form of the oral composition.

[0034] Dosage form / form The dosage form of the oral composition of this disclosure is not particularly limited as long as it can be taken orally, and may be solid (specifically, powder, granules, tablets, capsules, etc.), semi-solid (specifically, paste, gel, etc.), or liquid. Of these dosage forms, from the viewpoint of stability, etc., a solid form is preferred, preferably a capsule, and more preferably a soft capsule.

[0035] The formulation of the oral composition of this disclosure is not particularly limited as long as it can be taken orally, and may be either food or pharmaceutical, but food is preferred. Examples of food and beverages include foods for special dietary uses and health functional foods (nutrient function foods, foods with functional claims, foods for specified health uses, etc.), and general foods (nutritional supplements, health supplements, fortified foods, nutritional adjustment foods, supplements, etc.). Food and beverages include food and beverages for human use and feed for non-human animals (livestock feed, livestock supplements, pet food, pet supplements, etc.).

[0036] Purpose The oral compositions of this disclosure can be used in applications that utilize the known effects of Rhodiola rosea extract, and / or applications that utilize the known effects of lutein and / or zeaxanthin. The oral compositions of this disclosure are preferably used as antioxidants because they exhibit antioxidant effects at a level exceeding that of the amount of Rhodiola rosea extract present.

[0037] More specific uses of the oral composition disclosed herein include improving eye fatigue (for example, temporary eye fatigue caused by the use of smartphones, computers, etc.), improving sleep quality, improving sleep onset, improving sleep maintenance, anti-anxiety effects, stress reduction, improved relaxation, improved performance, improved concentration, improved rest effects, accelerated fatigue recovery, improvement of temporary gloomy moods before menstruation, and improvement of menstrual discomfort. [Examples]

[0038] The present disclosure will be described in detail below with reference to examples, but the present disclosure is not limited to these examples.

[0039] Test example Following the procedure for the DPPH antioxidant capacity measurement kit (Dojin Chemical Laboratories), assay buffer (included in the kit) and DPPH (1,1-diphenyl-2-picrylhydrazyl) solution were added to a 96-well microplate, and the components listed in Table 1 were added to reach the indicated final concentrations. For the Rhodiola rosea extract, a dried powder of the extract obtained by heating and refluxing dried Rhodiola rosea leaves with aqueous ethanol (containing 2% by weight of hyperoside and 2% by weight of isoquercitrin) was used. For the marigold extract, a suspension (containing 30% by weight of marigold extract, 20% by weight of lutein, and 4% by weight of zeaxanthin) obtained by heating and extracting dried marigold petals with hexane was used and suspended in sunflower oil. The marigold extract, as well as the lutein and zeaxanthin contained in the marigold extract, were added to the indicated amounts in the suspension.

[0040] DPPH is a stable, purple radical compound that becomes colorless upon reduction by antioxidants. The absorbance at 517 nm (at 25°C, 30 minutes after the start of measurement) was measured using a plate reader. The radical scavenging rate was evaluated as antioxidant capacity based on the following formula. The results are shown in Table 1.

[0041] [ka]

[0042] [Table 1]

[0043] As shown in Table 1, the antioxidant capacity of the combination of Rhodiola rosea extract (Comparative Examples 1 and 2) and lutein and zeaxanthin (Comparative Example 3) was synergistically improved.

[0044] Prescription examples The oral compositions shown in Table 2 were prepared, and 300 mg of each was placed in a soft capsule shell to create soft capsules. The resulting soft capsules exhibited improved antioxidant effects due to the inclusion of lutein and zeaxanthin.

[0045] [Table 2]

Claims

1. An oral composition comprising (A) Rhodiola rosea extract and (B) lutein and / or zeaxanthin.

2. The oral composition according to claim 1, wherein the total amount of component (B) is 0.001 to 20 parts by weight per 1 part by weight of component (A).

3. An oral composition according to claim 1 or 2, used as an antioxidant.

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