Cell culture vessels and related cell culture assemblies

Abstract

To provide a cell culture vessel that solves the problems of low operational efficiency and tendency for liquid to remain. [Solution] A cell culture vessel (11A) comprising a vessel portion (113A), a sloping neck (114A), a transition portion, at least one inlet port (111A), and at least one outlet port (112A), wherein the vessel portion includes a first vessel wall (1131A), a second vessel wall arranged opposite the first vessel wall, and a plurality of connecting walls (1133A) connecting the first vessel wall and the second vessel wall, wherein the vertical axis of the sloping neck is inclined at a predetermined angle with respect to the vertical axis of the vessel portion, wherein the transition portion connects the vessel portion and the sloping neck, and wherein the transition portion includes a first transition wall (1151A) connecting to the first vessel wall, a second transition wall connecting to the second vessel wall, and at least one third transition wall (1153A) connecting the first transition wall and the second transition wall, wherein the inlet port is provided in the first vessel wall or the first transition wall, and the outlet port is provided in one of the plurality of connecting walls or the second transition wall.

Description

[Technical Field]

[0001] The present invention relates to cell culture products, and more particularly to cell culture vessels and related cell culture assemblies. [Background technology]

[0002] In the field of biotechnology or cell culture, cell culture vessels, such as cell culture flasks, are one of the most commonly used basic consumables in laboratories for cell culture. Conventional cell culture vessels rely on a single opening in a canted neck to perform all operations, including adding fresh medium, seeding with cell suspensions, removing waste medium, and even rinsing with buffers such as PBS. This design is prone to low operational efficiency and residual liquid residue, which can affect the accuracy and reproducibility of experimental results. Therefore, improvements are needed. Summary of the Invention [Problem to be solved by the invention]

[0003] One object of the present invention is to provide a cell culture vessel and related cell culture assembly that solves the above problems. [Means for solving the problem]

[0004] To achieve the above object, the present invention discloses a cell culture vessel, the cell culture vessel comprising a vessel portion, a sloped neck, a transition portion, at least one inlet port, and at least one outlet port, the vessel portion including a first vessel wall, a second vessel wall, and a plurality of connecting walls, the second vessel wall being disposed opposite to the first vessel wall, the plurality of connecting walls connecting the first vessel wall and the second vessel wall, the longitudinal axis of the sloped neck being inclined at a predetermined angle with respect to the longitudinal axis of the vessel portion, and the transition portion being inclined forward. The transition portion connects the container portion and the inclined neck, and the transition portion includes a first transition wall, a second transition wall, and at least one third transition wall, wherein the first transition wall is connected to the first container wall, the second transition wall is connected to the second container wall, and the at least one third transition wall connects the first transition wall and the second transition wall, and the at least one inlet port is provided in the first container wall or the first transition wall, and the at least one outlet port is provided in one of the plurality of connecting walls or the second transition wall.

[0005] According to one embodiment of the present invention, the at least one inlet port is provided in the first container wall, and the at least one outlet port is provided in any of the plurality of connecting walls.

[0006] According to one embodiment of the present invention, the at least one inlet port is provided in the first transition wall, and the at least one outlet port is provided in any of the plurality of connecting walls.

[0007] According to one embodiment of the present invention, at least one guide structure is formed on any of the plurality of connecting walls, and the at least one guide structure is provided near the at least one outlet port to guide liquid toward the outlet port.

[0008] According to one embodiment of the present invention, the at least one guide structure is a funnel-shaped structure.

[0009] According to one embodiment of the present invention, the at least one inlet port is located in the first transition wall, and the at least one outlet port is located in the second transition wall.

[0010] According to one embodiment of the present invention, the at least one inlet port is provided in the first vessel wall, and the at least one outlet port is provided in the second transition wall.

[0011] According to one embodiment of the present invention, the second transition wall is formed with at least one guide structure, which is located near the at least one outlet port and is configured to guide liquid toward the outlet port.

[0012] According to one embodiment of the present invention, the at least one guide structure is a funnel-shaped structure.

[0013] According to one embodiment of the present invention, when the cell culture vessel is in a horizontal position, the second vessel wall contacts a support surface.

[0014] According to one embodiment of the present invention, when the cell culture vessel is in the horizontal position, the at least one inlet port is located outside the peripheral contour of the second vessel wall in vertical projection.

[0015] According to one embodiment of the present invention, the second transition wall includes an inclined portion inclined relative to the second container wall, and when the cell culture container is in the horizontal state, the at least one inlet port is located within the peripheral contour of the inclined portion of the second transition wall in a vertical projection.

[0016] According to one embodiment of the present invention, the second transition wall includes an extension step extending parallel to the second container wall, and when the cell culture container is in the horizontal state, a vertical projection of the at least one inlet port is located within the peripheral contour of the second container wall or the peripheral contour of the extension step.

[0017] According to one embodiment of the invention, the cross-sectional area of ​​the transition section gradually decreases in a direction from the container section to the inclined neck.

[0018] To achieve the above object, the present invention discloses a cell culture assembly, the cell culture assembly including a cell culture vessel, at least one inlet pipe, and at least one outlet pipe, the cell culture vessel having a vessel portion, a slanted neck, a transition portion, at least one inlet port, and at least one outlet port, the vessel portion including a first vessel wall, a second vessel wall disposed opposite to the first vessel wall, and a plurality of connecting walls connecting the first vessel wall and the second vessel wall, the longitudinal axis of the slanted neck being inclined at a predetermined angle with respect to the longitudinal axis of the vessel portion, the transition portion being inclined at a predetermined angle with respect to the vessel portion, The transition portion connects the container portion and the inclined neck, and the transition portion includes a first transition wall connected to the first container wall, a second transition wall connected to the second container wall, and at least one third transition wall connecting the first transition wall and the second transition wall, the at least one inlet port is provided in the first container wall or the first transition wall, the at least one outlet port is provided in one of the plurality of connecting walls or the second transition wall, the at least one inlet pipe is connected to the at least one inlet port, and the at least one outlet pipe is connected to the at least one outlet port. [Effects of the Invention]

[0019] As described above, in the present invention, the cell culture vessel communicates with upstream equipment (e.g., a cell culture medium reservoir, a cell detachment agent reservoir, a buffer solution reservoir, or a cell differentiation medium reservoir) via an inlet pipe, allowing liquid to flow into the cell culture vessel, thereby introducing the cell culture medium, cell detachment agent, buffer solution, or cell differentiation medium. The cell culture vessel also communicates with downstream equipment (e.g., a waste liquid reservoir or another cell culture vessel) via an outlet pipe, allowing liquid to be discharged from the cell culture vessel, thereby removing the cell culture medium or cell detachment agent. Furthermore, the cell culture vessel can be switched between a horizontal and non-horizontal position using a mechanical arm or a rotating mechanism. When the cell culture vessel is in a horizontal position, the inlet port faces upward, facilitating the introduction of the cell culture medium, cell detachment agent, buffer solution, or cell differentiation medium. On the other hand, when the cell culture vessel is in a non-horizontal position, the outlet port faces downward, allowing the cell culture medium or cell detachment agent to be removed from the cell culture vessel by gravity. Additionally, the inlet port can be connected to a gas source to supply gas to the cell culture vessel with the outlet port facing downward to assist in the removal of cell culture medium or cell release agent. [Brief explanation of the drawings]

[0020] [Figure 1] 1 is a schematic view showing the appearance of a cell culture assembly according to a first embodiment of the present invention; [Figure 2] 1 is an exploded view showing the components of a cell culture assembly according to a first embodiment of the present invention. FIG. [Figure 3] 1A to 1C are schematic views showing a cell culture vessel according to a first embodiment of the present invention from different viewing angles. [Figure 4] 1A to 1C are schematic views showing a cell culture vessel according to a first embodiment of the present invention from different viewing angles. [Figure 5] 1A to 1C are schematic views showing a cell culture vessel according to a first embodiment of the present invention from different viewing angles. [Figure 6] 1A to 1C are schematic diagrams illustrating the cell culture vessel according to the first embodiment of the present invention in different states. [Figure 7]1A to 1C are schematic diagrams illustrating the cell culture vessel according to the first embodiment of the present invention in different states. [Figure 8] 1A and 1B are schematic views showing a cell culture vessel according to a second embodiment of the present invention from different angles. [Figure 9] 1A and 1B are schematic views showing a cell culture vessel according to a second embodiment of the present invention from different angles. [Figure 10] 1A and 1B are schematic views showing a cell culture vessel according to a second embodiment of the present invention from different angles. [Figure 11] 5A-5C are schematic diagrams illustrating a cell culture vessel according to a second embodiment of the present invention in different states. [Figure 12] 5A-5C are schematic diagrams illustrating a cell culture vessel according to a second embodiment of the present invention in different states. [Figure 13] FIG. 10 is a plan view of a cell culture vessel according to a third embodiment of the present invention. [Figure 14] 10A-10C are schematic diagrams illustrating a cell culture vessel according to a third embodiment of the present invention in different states. [Figure 15] 10A-10C are schematic diagrams illustrating a cell culture vessel according to a third embodiment of the present invention in different states. [Figure 16] 10 is a plan view of a cell culture vessel according to a fourth embodiment of the present invention. FIG. [Figure 17] 10A-10C are schematic diagrams illustrating a cell culture vessel according to a fourth embodiment of the present invention in different states. [Figure 18] 10A-10C are schematic diagrams illustrating a cell culture vessel according to a fourth embodiment of the present invention in different states. DETAILED DESCRIPTION OF THE INVENTION

[0021] Directional terms used in the following examples, such as "upper," "lower," "left," "right," "front," and "rear," refer to directions shown in the accompanying drawings. Therefore, these directional terms are used for convenience of explanation and do not limit the present invention. Furthermore, unless otherwise specified, terms such as "connected" used in this specification include both direct and indirect electrical and structural connection means. Therefore, when a first device is described as being connected to a second device, this includes cases where the first device is directly electrically or structurally connected to the second device, as well as cases where the first device is indirectly electrically or structurally connected to the second device via other devices or connection means.

[0022] Reference will now be made to FIGS. 1 and 2. FIG. 1 is a schematic diagram showing the exterior of a cell culture assembly 1A according to a first embodiment of the present invention, and FIG. 2 is an exploded view showing the components of the cell culture assembly 1A according to the first embodiment of the present invention. As shown in FIGS. 1 and 2, the cell culture assembly 1A includes a cell culture vessel 11A, at least one inlet pipe 12A, and at least one outlet pipe 13A. The cell culture vessel 11A includes at least one inlet port 111A and at least one outlet port 112A. The inlet pipe 12A is connected to upstream equipment (e.g., a cell culture medium reservoir, a cell detachment agent reservoir, a buffer solution reservoir, or a cell differentiation medium reservoir), and the inlet port 111A is connected to the inlet pipe 12A. This allows liquid to flow from the upstream equipment into the cell culture vessel 11A, allowing the introduction of cell culture medium, cell detachment agent, buffer solution, or cell differentiation medium. Meanwhile, outlet tube 13A is connected to downstream equipment (e.g., a waste reservoir or another cell culture vessel), and outlet port 112A is connected to outlet tube 13A, allowing liquid to be discharged from cell culture vessel 11A to downstream equipment, allowing removal of cell culture medium or cell release agent. Specifically, in this embodiment, the cell culture assembly 1A has two inlet pipes 12A and two outlet pipes 13A, and the cell culture vessel 11A also has two inlet ports 111A and two outlet ports 112A. Note that the present invention is not limited to this embodiment, and the number of inlet pipes 12A, outlet pipes 13A, inlet ports 111A, and outlet ports 112A can be set arbitrarily according to design requirements. For example, in another embodiment, the cell culture assembly 1A may have one inlet pipe 12A and one outlet pipe 13A, and the cell culture vessel 11A may have one inlet port 111A and one outlet port 112A.

[0023] Please refer to Figures 2 to 7. Figures 3, 4, and 5 are schematic views showing the cell culture vessel according to the first embodiment of the present invention from different angles, and Figures 6 and 7 are schematic views showing the cell culture vessel according to the first embodiment of the present invention in different states. As shown in Figures 2 to 7, the cell culture vessel 11A includes a vessel portion 113A, a sloped neck 114A, and a transition portion 115A. The container portion 113A includes a first container wall 1131A, a second container wall 1132A, and a plurality of connecting walls 1133A. The second container wall 1132A is disposed opposite the first container wall, and the plurality of connecting walls 1133A connect the first container wall 1131A and the second container wall 1132A. The longitudinal axis L1 of the angled neck 114A is inclined at a predetermined angle X with respect to the longitudinal axis L2 of the container portion 113A. The transitional portion 115A connects the container portion 113A and the angled neck 114A, and the cross-sectional area of ​​the transitional portion 115A gradually decreases in the direction from the container portion 113A to the angled neck 114A. That is, the transitional portion 115A has a tapered structure. The transitional portion 115A further includes a first transitional wall 1151A, a second transitional wall 1152A, and at least one third transitional wall 1153A. The first transitional wall 1151A connects to the first container wall 1131A, the second transitional wall 1152A connects to the second container wall 1132A, and the third transitional wall 1153A connects to the first transitional wall 1151A and the second transitional wall 1152A. The inlet port 111A is provided in the first container wall 1131A, and the outlet port 112A is provided in one of the plurality of connecting walls 1133A that is disposed opposite the inclined neck 114A.

[0024] As shown in FIGS. 6 and 7, the cell culture vessel 11A can be switched between a horizontal position and a non-horizontal position by a mechanical arm or a rotation mechanism.

[0025] 6, the inlet port 111A faces upward, which facilitates the introduction of a cell culture medium, a cell release agent, a buffer solution, or a cell differentiation medium into the cell culture vessel 11A through the inlet tube 12A. In this state, the outer surface of the second vessel wall 1132A can be brought into contact with a support surface (e.g., a table surface or a platform surface of a mechanical arm), and the inner surface of the second vessel wall 1132A can be used as a surface for cells to adhere and culture.

[0026] 5 and 6, the second transitional wall 1152A includes an extending section S1A extending parallel to the second vessel wall 1132A and an inclined section S2A connected to the extending section S1A and inclined relative to the second vessel wall 1132A. When the cell culture vessel 11A is in a horizontal position, the vertical projection PA of the inlet port 111A is located at the intersection of the peripheral contour of the second vessel wall 1132A and the peripheral contour of the extending section S1A of the second transitional wall 1152A. However, the present invention is not limited to this embodiment. For example, in other embodiments, when the cell culture vessel 11A is in a horizontal position, the inlet port 111A may be positioned away from or towards the inclined neck 114A, and its vertical projection may be located within the peripheral contour of the second vessel wall 1132A or within the peripheral contour of the extension section S1A of the second transitional wall 1152A.

[0027] When the cell culture vessel 11A is in a non-horizontal position (e.g., a vertical position) as shown in Figure 7, the outlet port 112A faces downward, allowing the cell culture medium or cell release agent to be discharged from the cell culture vessel 11A by gravity through the outlet tube 13A.

[0028] In a preferred embodiment, inlet port 111A is connected to a gas source, and gas can be supplied into cell culture vessel 11A with outlet port 112A facing downward to assist in the removal of cell culture medium or cell release agent.

[0029] Furthermore, as shown in Figures 5 to 7, at least one guide structure GA is formed on the connecting wall 1133A located on the opposite side of the inclined neck 114A. The guide structure GA is disposed near the outlet port 112A and functions to guide the liquid to the outlet port 112A in the non-horizontal state shown in Figure 7. In this embodiment, the guide structure GA is a funnel-shaped structure. However, the present invention is not limited to this embodiment, and in other embodiments, the guide structure GA may be a slanted trough-shaped structure. In this embodiment, two guide structures GA are formed on the connecting wall 1133A located opposite the inclined neck 114A, and each guide structure GA is located adjacent to one of the two outlet ports 112A. However, the present invention is not limited to this embodiment. The number of guide structures GA may be changed according to design requirements. For example, in another embodiment, if the cell culture vessel 11A includes only one outlet port 112A, only one guide structure GA may be formed on the connecting wall 1133A located opposite the inclined neck 114A.

[0030] Please refer to Figures 8 to 12. Figures 8 to 10 are schematic views showing a cell culture vessel according to a second embodiment of the present invention from different viewing angles, and Figures 11 and 12 are schematic views showing the cell culture vessel according to the second embodiment of the present invention in different states. As shown in FIGS. 8 to 12, the second embodiment differs from the first embodiment in that at least one inlet port 111B is provided in a first transitional wall 1151B and at least one outlet port 112B is provided in a second transitional wall 1152B. Specifically, the second transitional wall 1152B includes only an inclined step S2B inclined relative to the second vessel wall 1132B. The outlet port 112B is provided in the inclined step S2B of the second transitional wall 1152B. When the cell culture vessel 11B is in a horizontal position, the inlet port 111B and the outlet port 112B are offset from each other in the vertical direction, i.e., the vertical projection PB of the inlet port 111B does not completely overlap with the outlet port 112B. Furthermore, when the cell culture vessel 11B is in a horizontal position, the vertical projection PB of the inlet port 111B is located outside the peripheral contour of the second vessel wall 1132B and within the peripheral contour of the sloped step S2B of the second transitional wall 1152B, to prevent the liquid flowing into the cell culture vessel 11B from directly washing away the cells adhering to the inner surface of the second vessel wall 1132B and detaching the cells. Additionally, at least one guide structure GB is formed on the sloped step S2B of the second transition wall 1152B. The guide structure GB is located near the outlet port 112B and functions to guide the liquid to the outlet port 112B. The rest of the structure of this embodiment is the same as that of the first embodiment, and there are various similar modifications, which will not be described in detail here for the sake of brevity.

[0031] Please refer to Figures 13 to 15. Figure 13 is a plan view of a cell culture vessel 11C according to a third embodiment of the present invention, and Figures 14 and 15 are schematic views showing the cell culture vessel 11C according to the third embodiment of the present invention in different states. As shown in Figures 13 to 15, the third embodiment is different from the first embodiment in that at least one inlet port 111C is provided in the first transition wall 1151C, and at least one outlet port 112C is provided in the connecting wall 1133C located on the opposite side of the inclined neck 114C. Furthermore, the second transitional wall 1152C includes only an inclined step S2C inclined relative to the second container wall 1132C. When the cell culture container 11C is in a horizontal position, the vertical projection PC of the inlet port 111C is located outside the peripheral contour of the second container wall 1132C and within the peripheral contour of the inclined step S2C of the second transitional wall 1152C. This prevents the liquid flowing into the cell culture container 11C from directly washing away cells attached to the inner surface of the second container wall 1132C and detaching the cells. Furthermore, at least one guide structure GC is formed on the connecting wall 1133C located on the opposite side of the inclined neck 114C. The guide structure GC is located near the outlet port 112C and functions to guide the liquid to the outlet port 112C. The rest of the structure of this embodiment is the same as that of the first embodiment, and there are various similar modifications, which will not be described in detail here for the sake of brevity.

[0032] Please refer to Figures 16 to 18. Figure 16 is a plan view of a cell culture vessel 11D according to a fourth embodiment of the present invention, and Figures 17 and 18 are schematic views showing the cell culture vessel 11D according to the fourth embodiment of the present invention in different states. As shown in Figures 16 to 18, the fourth embodiment is different from the first embodiment in that the second transition wall 1152D in the fourth embodiment includes only a sloped step S2D inclined relative to the second vessel wall 1132D. At least one inlet port 111D is provided in the first vessel wall 1131D, and at least one outlet port 112D is provided in the sloped step S2D of the second transition wall 1152D. Furthermore, when the cell culture vessel 11D is in a horizontal position, the vertical projection PD of the inlet port 111D is located within the peripheral contour of the second vessel wall 1132D, and at least one guide structure GD is formed on the sloped step S2D of the second transitional wall 1152D. The guide structure GD is located near the outlet port 112D and functions to guide the liquid to the outlet port 112D. The rest of the structure of this embodiment is the same as that of the first embodiment, and there are various similar modifications, which will not be described in detail here for the sake of brevity.

[0033] Compared with the prior art, in the present invention, the cell culture vessel is connected to an upstream device (e.g., a cell culture medium reservoir, a cell detachment agent reservoir, a buffer solution reservoir, or a cell differentiation medium reservoir) through an inlet pipe, allowing liquid to flow into the cell culture vessel, thereby introducing the cell culture medium, cell detachment agent, buffer solution, or cell differentiation medium. The cell culture vessel may also be connected via an outlet tube to downstream equipment (e.g., a waste reservoir or another cell culture vessel) to allow liquid to be drained from the cell culture vessel and for cell culture medium or cell release agent to be removed. Furthermore, the cell culture vessel can be switched between a horizontal and a non-horizontal position by a mechanical arm or a pivoting mechanism. When the cell culture vessel is in a horizontal position, the inlet port faces upward, allowing for easy introduction of cell culture medium, cell detachment agent, buffer solution, or cell differentiation medium into the cell culture vessel. When the cell culture vessel is in a non-horizontal position, the outlet port faces downward, allowing for the removal of cell culture medium or cell detachment agent from the cell culture vessel by gravity. Additionally, the inlet port can be connected to a gas source to supply gas to the cell culture vessel when the outlet port faces downward to aid in the removal of cell culture medium or cell release agent. [Explanation of symbols]

[0034] 1A: Cell culture assembly 11A, 11B, 11C, 11D: Cell culture container 12A: Inlet pipe 13A: Outlet pipe 111A, 111B, 111C, 111D: Inlet ports 112A, 112B, 112C, 112D: Outlet port 113A: Container part 1131A, 1131D: First container wall 1132A, 1132B, 1132C, 1132D: Second container wall 1133A, 1133C: Connecting wall 114A, 114C: Inclined neck 115A:Transient section 1151A, 1151B, 1151C: First transition wall 1152A, 1152B, 1152C, 1152D: Second transition wall 1153A: Third transition wall GA, GB, GC, GD: ガイド structure L1, L2: Vertical shafts PA, PB, PC, PD: Projection S1A: Extension Section S2A, S2B, S2C, S2D: Inclined sections X: determined angle

Claims

1. A cell culture vessel, comprising: a vessel portion, a sloped neck, a transition portion, at least one inlet port, and at least one outlet port; The container portion is a first vessel wall; a second container wall disposed opposite the first container wall; a plurality of connecting walls connecting the first container wall and the second container wall; The longitudinal axis of the inclined neck is inclined at a predetermined angle relative to the longitudinal axis of the container portion, the transition portion connects the container portion and the sloped neck; The transition portion is a first transition wall coupled to the first vessel wall; a second transition wall coupled to the second vessel wall; at least one third transition wall connecting the first transition wall and the second transition wall; the at least one inlet port is located in the first vessel wall or the first transition wall; The cell culture vessel, wherein the at least one outlet port is provided in one of the plurality of connecting walls or the second transition wall.

2. The cell culture vessel of claim 1 , wherein the at least one inlet port is provided in the first vessel wall, and the at least one outlet port is provided in any one of the plurality of connecting walls.

3. The cell culture vessel of claim 1 , wherein the at least one inlet port is provided in the first transition wall, and the at least one outlet port is provided in any one of the plurality of connecting walls.

4. 4. The cell culture vessel according to claim 2, wherein at least one guide structure is formed in any one of the plurality of connecting walls, and the at least one guide structure is provided in the vicinity of the at least one outlet port for guiding a liquid toward the outlet port.

5. The cell culture vessel of claim 4 , wherein the at least one guide structure is a funnel-shaped structure.

6. 2. The cell culture vessel of claim 1, wherein the at least one inlet port is located in the first transitional wall and the at least one outlet port is located in the second transitional wall.

7. 2. The cell culture vessel of claim 1, wherein the at least one inlet port is located in the first vessel wall and the at least one outlet port is located in the second transitional wall.

8. The cell culture vessel according to claim 6 or 7, wherein the second transitional wall is formed with at least one guide structure, the at least one guide structure being provided in the vicinity of the at least one outlet port for guiding liquid toward the outlet port.

9. The cell culture vessel of claim 8 , wherein the at least one guide structure is a funnel-shaped structure.

10. The cell culture vessel of claim 1 , wherein the second vessel wall contacts a support surface when the cell culture vessel is in a horizontal position.

11. The cell culture vessel of claim 10 , wherein when the cell culture vessel is in the horizontal position, the at least one inlet port is located outside the outer peripheral edge contour of the second vessel wall when projected vertically.

12. 11. The cell culture vessel of claim 10, wherein the second transition wall includes a sloped step inclined relative to the second vessel wall, and when the cell culture vessel is in the horizontal state, the at least one inlet port is located within a peripheral contour of the sloped step of the second transition wall as projected vertically.

13. 11. The cell culture vessel of claim 10, wherein the second transitional wall includes an extension step extending parallel to the second vessel wall, and when the cell culture vessel is in the horizontal position, the at least one inlet port is located, as projected vertically, either inside a peripheral contour of the second vessel wall, inside a peripheral contour of the extension step of the second transitional wall, or at an intersection of the peripheral contour of the second vessel wall and the peripheral contour of the extension step of the second transitional wall.

14. The cell culture vessel of claim 1 , wherein the cross-sectional area of ​​the transitional portion gradually decreases in a direction from the vessel portion toward the angled neck.

15. 1. A cell culture assembly comprising: a cell culture vessel, at least one inlet tube, and at least one outlet tube; the cell culture vessel comprising a vessel portion, a sloped neck, a transition portion, at least one inlet port, and at least one outlet port; The container portion is a first vessel wall; a second container wall disposed opposite the first container wall; a plurality of connecting walls connecting the first container wall and the second container wall; The longitudinal axis of the inclined neck is inclined at a predetermined angle relative to the longitudinal axis of the container portion, the transition portion connects the container portion and the sloped neck; The transition portion is a first transition wall coupled to the first vessel wall; a second transition wall coupled to the second vessel wall; at least one third transition wall connecting the first transition wall and the second transition wall; the at least one inlet port is located in the first vessel wall or the first transition wall; the at least one outlet port is provided in one of the plurality of connecting walls or the second transition wall; the at least one inlet pipe is connected to the at least one inlet port; The at least one outlet tube is connected to the at least one outlet port.

Images