Virus detection method, detection system, and detection kit

Abstract

A mask (1) includes an antibody layer (11) on which an antibody (2) capable of binding to a virus (3) is fixed. A detection method comprises: a step for attaching a label (4) including a site (41) that is bound to the virus (3) and a site (42) which receives excitation light to emit fluorescent light to the antibody layer (11) in the mask (1) that a subject wears (step (3)); and a step for irradiating the antibody layer (11) with the excitation light (step (5)).

Description

[Technical field]

[0001] The present invention relates to a virus detection method, detection system, and detection kit, and more particularly to a technology for detecting a virus using a mask after it has been worn by a subject. [Background technology]

[0002] In order to prevent droplet and airborne infection of infectious diseases caused by viruses, it is common to protect the mouth and nose with a mask made of nonwoven fabric or the like. Non-Patent Document 1 discloses research on embedding a genetic circuit inside the mask to create a biosensor that changes color when it detects target virus molecules. With this configuration, it is easy to determine whether a subject wearing the mask is infected with the target virus. [Prior art documents] [Non-patent literature]

[0003] [Non-Patent Document 1] Peter Q. Nguyen et al., "Wearable materials with embedded synthetic biology sensors for biomolecule detection", Nature biotechnology, 28 June 2021, https: / / doi.org / 10.1038 / s41587-021-00950-3) Summary of the Invention [Problem to be solved by the invention]

[0004] On the other hand, the configuration disclosed in Non-Patent Document 1 requires the manufacture of a mask in which a special genetic circuit is embedded, which may increase the cost of the mask.

[0005] The present disclosure has been made to solve such problems, and its purpose is to detect viruses using a mask with a simple configuration. [Means for solving the problem]

[0006] A first aspect of the present invention relates to a method for detecting a virus using a mask, the mask including an antibody layer having an antibody that binds to the virus immobilized thereon, the method comprising the steps of: adding a label to the antibody layer of the mask worn by a subject, the label including a site that binds to the virus and a site that emits fluorescence upon receiving excitation light; and irradiating the antibody layer with excitation light.

[0007] A second aspect of the present invention relates to a virus detection system using a mask. The mask includes an antibody layer to which antibodies that bind to viruses are fixed. The detection system includes a label adder, an illuminator, and a detector. The label adder adds a label to the antibody layer of the mask worn by the subject, the label including a site that binds to the virus and a site that emits fluorescence upon receiving excitation light. The illuminator irradiates the antibody layer with excitation light. The detector detects the fluorescence emitted from the antibody layer by the excitation light.

[0008] A third aspect of the present invention relates to a virus detection kit. The detection kit includes a mask and a label adder. The mask includes an antibody layer to which an antibody that binds to the virus is fixed. The label adder adds a label to the antibody layer of the mask worn by the subject, the label including a site that binds to the virus and a site that emits fluorescence upon receiving excitation light. Effect of the Invention

[0009] According to the present disclosure, viruses that are discharged from the subject's mouth or nose and bind to the antibody layer of the mask can be detected by the fluorescence emitted from the label. In this way, viruses can be detected using a mask with a simple configuration that has an antibody layer. [Brief description of the drawings]

[0010] [Figure 1]1 is a diagram illustrating an overview of a virus detection system according to an embodiment of the present invention. [Diagram 2] 1 is a diagram illustrating a method for detecting a virus according to an embodiment of the present invention. [Diagram 3] 1 is a diagram illustrating a configuration of a virus detection system and a detection device according to an embodiment of the present invention. [Figure 4] 11 is a flowchart illustrating a process related to virus detection. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0011] Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. In the drawings, the same or corresponding parts are designated by the same reference characters and their description will not be repeated.

[0012] [1. Overview of the virus detection system] FIG. 1 is a diagram illustrating an overview of a virus detection system 100 according to an embodiment of the present invention.

[0013] 1, the detection system 100 includes a label adding device 51, a label removing device 52, and a detection unit 6. In order to detect a virus in the detection system 100, a mask 1 is used.

[0014] The mask 1 includes an antibody layer 11 having an antibody 2 fixed thereon that binds to a virus 3. The antibody layer 11 is disposed, for example, on the surface (inner side) of the mask 1 facing the subject. With this configuration, viruses 3 discharged from the respiratory system of the subject bind to the antibody layer 11. By disposing the antibody layer 11 on the inner side of the mask 1, viruses discharged from people other than the subject are less likely to bind to the antibody layer 11 compared to when the antibody layer 11 is disposed on the outer side of the mask 1. The mask 1 may include a filter for collecting viruses discharged from people other than the subject, outside the antibody layer 11. The mask 1 may be, for example, a mask in which the antibody layer 11 is layered on the subject-side surface of a general polypropylene nonwoven mask.

[0015] The label adder 51 is used to add the label 4 to the antibody layer 11 of the mask 1 worn by the subject. The label 4 includes a site 41 that binds to the virus 3 and a site 42 that emits fluorescence upon receiving excitation light. The label adder 51 is, for example, a spray bottle that stores a label liquid 45 containing the label 4. The label liquid 45 is a liquid suitable for keeping the label 4 stable in the label liquid 45, and is, for example, a buffer solution to which the label 4 and, if necessary, a component that prevents aggregation of the label 4 have been added.

[0016] The label remover 52 is used to remove the label 4 that is not bound to the antibody layer 11. The label remover 52 is, for example, a tool including a fan 521.

[0017] The label adder 51 and the label remover 52 may be one unit related to label addition, which may be referred to as the label adding unit 5. The label adding unit 5 may include other instruments. The label adding unit is, for example, a clean bench or a laboratory table including the label adder 51 and the label remover 52.

[0018] The detection unit 6 includes an illuminator 61 and a detector 62. The illuminator 61 irradiates the antibody layer 11 housed in the detection unit with excitation light. The detector 62 detects fluorescence generated from the antibody layer 11 by the excitation light. The detection unit 6 is, for example, a general fluorescence detection device.

[0019] [2. Comparison with conventional virus detection methods] A known method for detecting viruses using a mask is to incorporate a genetic circuit into the mask so that it detects viral RNA and changes color (Non-Patent Document 1). However, this detection method requires the preparation of a mask incorporating a special genetic circuit, which could increase the cost of the mask and, ultimately, the cost of the detection method itself.

[0020] Therefore, in the virus detection method according to the present embodiment, a mask including an antibody layer on which antibodies bound to viruses are fixed is used. An ostrich antibody mask is known as such a mask including an antibody layer. Specifically, a label including a site that binds to viruses and a site that emits fluorescence upon receiving excitation light is added to the antibody layer of the mask worn by the subject by a label adder. If viruses are bound to the antibody layer, fluorescence is generated when the antibody layer is irradiated with excitation light. Therefore, in the virus detection method according to the present embodiment, a mask with a simple configuration can be used to detect viruses.

[0021] [3. Virus detection methods] 2 is a diagram illustrating a virus detection method according to an embodiment of the present invention. With reference to FIG. 2, (1) to (5) show the order of each step of the virus detection method.

[0022] In FIG. 2(1), the subject prepares a mask 1. In FIG. 2( 2 ), when a subject wears a mask 1 , viruses 3 excreted from the subject's mouth or nose bind to antibodies 2 in the antibody layer 11 .

[0023] The subsequent steps of FIG. 2(3) to (5) are performed by, for example, a medical technician. In Fig. 2(3), a laboratory technician applies a label 4 to the antibody layer 11 of the mask 1 worn by the subject using a label applicator 51. For example, the label 4 is applied by spraying a label liquid 45, which is a liquid containing the label 4, from the label applicator 51.

[0024] When the label 4 is added to the antibody layer 11 by the label adder 51, some of the labels 4 bind to the viruses 3 at the sites 41. In this embodiment, the labels 4 bound to the viruses 3 are referred to as "bound labels 4A." Meanwhile, some of the labels 4 remain unbound to the viruses 3 and are present in the antibody layer 11. In this embodiment, the labels 4 not bound to the viruses 3 are referred to as "unbound labels 4B." The unbound labels 4B are attached, for example, to portions of the antibody layer 11 other than the viruses 3.

[0025] 2(4), the laboratory technician uses a label remover 52 to remove unbound labels 4B from the antibody layer 11. As a result, the only labels 4 remaining in the antibody layer 11 are bound labels 4A bound to the viruses 3.

[0026] The label remover 52 rotates a fan 521 to suck or blow away the unbound labels 4B and remove them from the antibody layer 11. The exhaust air containing the unbound labels 4B generated by the label remover 52 is filtered by an air filter such as a HEPA filter (High Efficiency Particulate Air Filter) and exhausted to the outside.

[0027] In FIG. 2(5), the laboratory technician places the antibody layer 11 in the detection unit 6. The laboratory technician irradiates the antibody layer 11 with excitation light from an irradiator 61, and detects the fluorescence generated from the site 42 of the binding label 4A by the excitation light using a detector 62. In this way, the viruses 3 bound to the antibody layer 11 can be detected by detecting the fluorescence of the binding label 4A bound to the viruses 3. In other words, it can be determined whether or not the subject wearing the mask 1 is infected with the viruses 3.

[0028] In FIG. 2(5), the excitation light irradiated from the irradiator 61 may cause not only the binding label 4A but also the material constituting the mask 1 to generate light. For example, polypropylene nonwoven fabric, which is widely used in general nonwoven masks, is known to generate fluorescence when excited by ultraviolet light. If the wavelength range of the fluorescence generated by the nonwoven fabric overlaps with the wavelength range of the fluorescence generated by the binding label 4A, viruses may not be detected accurately. In this embodiment, light in the wavelength range of the near-infrared region can be used as the excitation light irradiated from the irradiator 61. In this case, even if the mask 1 is configured to include a portion of polypropylene nonwoven fabric, the polypropylene nonwoven fabric itself does not generate fluorescence, so it does not interfere with the detection of the fluorescence from the binding label 4A in the antibody layer 11.

[0029] 2(3) and subsequent steps may be performed by the laboratory technician on the "portion including the antibody layer 11" isolated from the mask 1. For example, if the laboratory technician removes the parts of the mask that hang from the ears, the remaining part becomes easier to handle.

[0030] As described above, a filter for capturing viruses excreted from humans other than the subject may be included outside the antibody layer 11 of the mask 1. In this case, for example, removing the filter before the step of Fig. 2(3) has the advantage that the viruses captured by the filter do not interfere with the detection of the viruses 3 bound to the antibody layer 11.

[0031] Furthermore, if the subject has the skills to perform the steps in FIG. 2(3) and subsequent steps, the subject may perform these steps by himself or herself.

[0032] 2(4), the unbound label 4B may be removed from the antibody layer 11, for example, by washing the antibody layer 11 with a rinse solution. The rinse solution is a liquid suitable for washing the antibody layer 11, for example, a buffer solution.

[0033] Label 4 binds to virus 3 Even if you don'tThe first type of label may be one that emits fluorescence and binds to the virus 3. Only in this state The second type of label may be one that generates fluorescence. Known labels can be used for both the first type of label and the second type of label.

[0034] The first type of label has the advantage of being relatively inexpensive. An example of the first type of label is indocyanine green (ICG). It is known that ICG also emits fluorescence when excited by light in the near-infrared wavelength range.

[0035] Although the second type of label tends to be more expensive than the first type of label, it has the advantage that the step of removing unbound labels shown in Figure 2(4) can be omitted because it does not emit fluorescence even when irradiated with excitation light in the unbound state.

[0036] In the above example, the mask 1 and the label 4 stored in the label adder 51 are consumables. At least one of these consumables, the mask 1 and the label 4 stored in the label adder 51, may be configured to be replenished by a laboratory technician or a subject as appropriate. For example, the mask 1 and the label liquid 45 may be configured to be sold as a virus 3 detection kit to subjects and facilities to which the subjects belong.

[0037] [4. Configuration of detection system and detection device] Fig. 3 is a diagram illustrating the configurations of virus detection system 100 and detection device 100A according to an embodiment of the present invention. With reference to Fig. 3, virus detection system 100 outlined in Fig. 1 will be described in more detail, and then a configuration in the case where virus detection system 100 is realized as detection device 100A will be described.

[0038] 1 and 2, the label adding device 51 of the label adding unit 5 is illustrated as a spray bottle containing the label liquid 45, but is not limited thereto. Also, the label remover 52 is illustrated as a device including a fan 521, but is not limited thereto.

[0039] For example, the label adding unit 5 may be a single device including the label adder 51 and the label remover 52. In this case, for example, the label adding unit 5 has a lattice-shaped table for placing the mask 1 therein. The label adding unit 5 includes a sprayer for the label liquid 45, which is one form of the label adder 51, above the table. The label adding unit 5 also includes an aspirator, which is one form of the label remover 52, at a position above the table that does not overlap with the sprayer. The sprayer and the aspirator are controlled, for example, by a control unit described later. In this case, for example, when a laboratory technician stores the mask 1 or the "part including the antibody layer 11" in the label adding unit 5, the sprayer automatically sprays the label liquid 45 onto the antibody layer 11, and then the aspirator aspirates the unbound labels 4B.

[0040] The detection unit 6 includes the illuminator 61 and the detector 62 described in Fig. 1. The detection unit 6 may further include an alarm 63. The alarm 63 is a device that visually and / or audibly notifies a laboratory technician of the detection of the virus 3 in the detection unit 6. The detection of the virus 3 is determined, for example, by whether or not the intensity of the detected fluorescence exceeds a threshold value.

[0041] The alarm 63 is, for example, a display, a lamp, or a speaker. The alarm 63 is provided, for example, on the outside of the fluorescence detection device including the irradiator 61 and the detector 62. With this configuration, the laboratory technician can easily recognize that the virus 3 has been detected by the detector 62.

[0042] Such a detection system 100 can perform the virus detection method described with reference to FIG.

[0043] The detection system 100 may be realized as a detection device 100A including a labeling unit 5 and a detection unit 6. In this case, for example, the detection device 100A includes a control unit 7 in addition to the labeling unit 5 and the detection unit 6. The control unit 7 controls each unit. The control unit 7 includes, for example, a processor 71 and a memory 72, which are basic components of a computer. The processor 71 and the memory 72 are connected to each other by a common bus 73. The detection device 100A may further include an input unit for a laboratory technician or a subject to operate the detection device 100A, and a handling device for inserting / removing the mask 1 or the "part including the antibody layer 11" into / from the labeling unit 5 and the detection unit 6. In the detection device 100A, the labeling unit 5 and the detection unit 6 may be provided as separate bodies (for example, two housings) or as an integrated body (for example, one housing).

[0044] In the detection device 100A, for example, when the mask 1 or "a portion including the antibody layer 11" is stored, the above-mentioned series of virus detection methods are automatically executed. In this case, the storage in the detection device 100A and the operation of the detection device 100A may be performed by the subject himself, not by a laboratory technician. In other words, the detection device 100A allows the subject to self-check for infection with the virus 3.

[0045] Therefore, for example, if the detection device 100A is installed at the entrance of the facility to which the subject belongs, the subject can store the mask 1 in the detection device 100A when entering or leaving the facility, and perform the self-check. In this case, when the alarm 63 notifies that the virus 3 has been detected in the antibody layer 11, the subject may be prevented from entering or leaving the facility. The detection device 100A may be configured to include a device for identifying the subject (for example, an optical camera or an ID card sensor for the subject at the facility) so that a subject infected with the virus 3 can be identified. Such a configuration helps prevent the spread of infection of the virus 3 inside and outside the facility.

[0046] [5. Processing flow in the detection device] Fig. 4 is a flow chart for explaining the process of detecting the virus 3 by the detection device 100A. The process of Fig. 4 is executed by the control unit 7 of the detection device 100A.

[0047] Referring to FIG. 4, in step ST02, the control unit 7 detects that the antibody layer 11 or the mask 1 including the antibody layer 11 has been stored in the detection device 100A. The storage is detected, for example, by using a weight sensor (not shown) attached to a location in the detection device 100A where the antibody layer 11 or the mask 1 including the antibody layer 11 is stored. In step ST04, the control unit 7 adds the label 4 to the antibody layer 11 using the label adder 51. In step ST06, the control unit 7 removes the unbound label 4B from the antibody layer 11 using the label remover 52. In step ST08, the control unit 7 irradiates the antibody layer 11 with excitation light using the irradiator 61. In step ST10, the control unit 7 detects fluorescence generated from the bound label 4A bound to the antibody layer 11 using the detector 62. In step ST12, the control unit 7 notifies the detector 62 that the fluorescence has been detected using the alarm 63.

[0048] Therefore, if the control unit 7 of the detection device 100A executes the process according to the flowchart of Fig. 4, it is possible to detect the viruses 3 attached to the antibody layer 11. That is, the viruses 3 can be detected using the mask 1 with a simple configuration.

[0049] In this manner, the virus detection method, detection system 100, and detection device 100A according to this embodiment can use a mask 1 of simple configuration to test a subject wearing the mask 1 for an infectious disease caused by a virus 3.

[0050] Typically, infectious disease tests are conducted to confirm whether or not someone is infected when specific symptoms of the disease are observed. However, there are some infectious diseases, such as the novel coronavirus disease (COVID-19), that are often asymptomatic even when infected. In such cases, asymptomatic infected people do not have the opportunity to be tested, and there is a risk that they may unknowingly spread the infection to those around them.

[0051] On the other hand, in the virus detection method, detection system 100, and detection device 100A according to the present embodiment, testing for infection with the virus 3 is easy, inexpensive, and does not impose a physical burden on the subject. Therefore, it is easy to test many subjects, including asymptomatic subjects. In other words, by expanding the opportunities to test whether asymptomatic persons are infected and at risk of infecting others, it is possible to suppress the unconscious spread of infection by asymptomatic persons.

[0052] [Aspects] It will be appreciated by those skilled in the art that the exemplary embodiments described above are examples of the following aspects.

[0053] (Item 1) A method for detecting a virus according to one embodiment comprises: A method for detecting a virus using a mask, comprising: The mask includes an antibody layer, which is a layer to which antibodies that bind to the virus are fixed, The detection method is Adding a label to the antibody layer of the mask worn by the subject, the label including a site that binds to a virus and a site that emits fluorescence upon receiving excitation light; and irradiating the antibody layer with excitation light.

[0054] According to the virus detection method described in paragraph 1, the virus excreted from the subject's mouth or nose binds to the antibody layer of the mask. The bound virus is detected by a label that emits fluorescence. Therefore, the virus can be detected using a mask with a simple structure.

[0055] (Item 2) In the virus detection method described in item 1, the label has the property of emitting fluorescence when exposed to excitation light regardless of binding to the virus, and the method may further include a step of removing unbound labels, which are labels that are not bound to the virus, from the antibody layer.

[0056] According to the virus detection method described in paragraph 2, the only labels present in the antibody layer are the bound labels bound to the viruses. Therefore, when the antibody layer is irradiated with excitation light, the fluorescence of only the bound labels bound to the viruses can be detected, and the influence of the unbound labels can be eliminated. Therefore, the viruses bound to the antibody layer can be detected.

[0057] (Item 3) In the virus detection method described in Item 2, the step of removing unbound labels may include removing the unbound labels from the antibody layer by sucking or blowing away the unbound labels.

[0058] According to the virus detection method described in paragraph 3, the labels present in the antibody layer are only the bound labels bound to the viruses. Therefore, when the antibody layer is irradiated with excitation light, the fluorescence of only the bound labels bound to the viruses can be detected, and the influence of unbound labels can be eliminated. Therefore, the viruses bound to the antibody layer can be detected. Furthermore, the exhaust air containing unbound labels generated by the label remover can be easily exhausted to the outside by filtering it with an air filter such as a HEPA filter.

[0059] (Item 4) In the virus detection method described in Item 2, the step of removing the unbound label may include a step of removing the unbound label from the antibody layer by washing the antibody layer with a rinsing solution.

[0060] According to the virus detection method described in paragraph 4, the only labels present in the antibody layer are the bound labels bound to the viruses. Therefore, when the antibody layer is irradiated with excitation light, the fluorescence of only the bound labels bound to the viruses can be detected, and the influence of the unbound labels can be eliminated. Therefore, the viruses bound to the antibody layer can be detected. Furthermore, the unbound labels can be removed, for example, using a rinse solution used for washing after a normal antibody-antigen reaction. Therefore, there is no need to prepare a special tool or technique for the removal.

[0061] (Item 5) In the virus detection method according to any one of Items 1 to 4, the excitation light may be light in the near-infrared wavelength range.

[0062] According to the virus detection method described in paragraph 5, even if excitation light is applied to polypropylene nonwoven fabric, which is widely used in general nonwoven fabric masks, no fluorescence is generated. Therefore, even if a mask containing polypropylene nonwoven fabric is used, the fluorescence from the bound label in the antibody layer can be appropriately detected.

[0063] (Item 6) In the virus detection method according to any one of items 1 to 5, the antibody layer may be disposed on the surface of the mask facing the subject.

[0064] According to the method for detecting a virus described in item 6, the virus excreted from the respiratory system of the subject becomes more likely to bind to the antibody layer.

[0065] (Item 7) A virus detection system according to one embodiment comprises: A virus detection system using a mask, The mask includes an antibody layer, which is a layer to which antibodies that bind to the virus are fixed, The detection system comprises: a label adder that adds a label to the antibody layer of the mask worn by the subject, the label including a site that binds to the virus and a site that emits fluorescence upon receiving excitation light; an irradiator for irradiating the antibody layer with excitation light; and a detector for detecting fluorescence emitted from the antibody layer by the excitation light.

[0066] According to the virus detection system described in paragraph 7, the virus excreted from the subject's mouth or nose binds to the antibody layer of the mask. The bound virus is detected by a label that emits fluorescence. Therefore, the virus can be detected using a mask with a simple structure.

[0067] (Item 8) The virus detection system according to item 7 may further include a label remover that removes unbound labels, which are labels that are not bound to the virus, from the antibody layer.

[0068] According to the virus detection system described in paragraph 8, the only labels present in the antibody layer are the bound labels bound to the viruses. Therefore, when the antibody layer is irradiated with excitation light, the fluorescence of only the bound labels bound to the viruses can be detected, and the influence of the unbound labels can be eliminated. Therefore, the viruses bound to the antibody layer can be detected.

[0069] (Item 9) The virus detection system according to item 7 or 8 may further include an alarm that notifies the user that fluorescence has been detected by the detector.

[0070] According to the virus detection system described in paragraph 9, a laboratory technician can easily recognize that a virus has been detected in the detector.

[0071] (Item 10) A virus detection kit according to one embodiment includes: A mask including an antibody layer having an antibody that binds to the virus fixed thereto; The mask may also include a label adder that adds a label to the antibody layer of the mask worn by the subject, the label including a site that binds to the virus and a site that emits fluorescence when exposed to excitation light.

[0072] According to the virus detection kit described in paragraph 10, viruses excreted from the subject's mouth or nose bind to the antibody layer of the mask. A fluorescent label is attached to the bound viruses. Therefore, by irradiating the antibody layer of the mask with excitation light, the viruses attached to the antibody layer can be detected using fluorescence. Therefore, viruses can be detected using a mask with a simple structure.

[0073] The embodiments disclosed herein should be considered to be illustrative and not restrictive in all respects. The scope of the present invention is defined by the claims, not the above description, and is intended to include all modifications within the meaning and scope of the claims. [Explanation of symbols]

[0074] 1 mask, 2 antibody, 3 virus, 4 label, 4A bound label, 4B unbound label, 5 label addition unit, 6 detection unit, 7 control unit, 11 antibody layer, 41, 42 site, 45 label solution, 51 label addition device, 52 label removal device, 61 irradiator, 62 detector, 63 alarm, 71 processor, 72 memory, 73 bus, 100 detection system, 100A detection device, 521 fan.

Claims

1. A method for detecting a virus using a mask, comprising: The mask includes an antibody layer to which an antibody that binds to the virus is fixed, The detection method includes: A step of spraying a label liquid containing a label having a site that binds to the virus and a site that emits fluorescence upon receiving excitation light onto the antibody layer of the mask worn by the subject; A virus detection method comprising a step of irradiating the antibody layer with excitation light.

2. the label has a property of emitting fluorescence upon receiving the excitation light regardless of binding to the virus, The method for detecting a virus according to claim 1 , further comprising the step of removing unbound labels, which are labels that are not bound to the virus, from the antibody layer.

3. The method for detecting a virus according to claim 2 , wherein the step of removing the unbound labels comprises the step of removing the unbound labels from the antibody layer by sucking or blowing the unbound labels away.

4. The method for detecting a virus according to claim 2 , wherein the step of removing the unbound label comprises the step of removing the unbound label from the antibody layer by washing the antibody layer with a rinsing solution.

5. The virus detection method according to claim 1 , wherein the excitation light is light in the near-infrared wavelength range.

6. The method for detecting a virus according to claim 1 , wherein the antibody layer is placed on the surface of the mask facing the subject.

7. A virus detection system using a mask, The mask includes an antibody layer to which an antibody that binds to the virus is fixed, The detection system comprises: a label adding device that sprays a label liquid containing a label having a site that binds to the virus and a site that emits fluorescence when exposed to excitation light onto the antibody layer of the mask worn by the subject; an irradiator for irradiating the antibody layer with excitation light; A virus detection system comprising: a detector that detects fluorescence generated from the antibody layer in response to the excitation light.

8. The virus detection system according to claim 7 , further comprising a label remover for removing unbound labels, which are labels that are not bound to the virus, from the antibody layer.

9. The virus detection system according to claim 7 , further comprising an alarm that notifies that fluorescence has been detected by the detector.

10. A virus detection kit, comprising: A mask including an antibody layer having an antibody that binds to the virus immobilized thereon; A virus detection kit comprising: a label adder that sprays a label liquid containing a label that has a site that binds to the virus and a site that emits fluorescence when exposed to excitation light onto the antibody layer of the mask worn by a subject.

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