Flow cell containing heteropolymer

Abstract

The flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer having a linking group reactive with the functional group attached to the primer, and a monomer having a stimulus-responsive functional group. The monomer having a stimulus-responsive functional group may be pH-responsive, temperature-responsive, sugar-responsive, nucleophile-responsive, and / or salt-responsive.

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Application No. 62 / 793,250, filed January 14, 2019, the contents of which are incorporated herein by reference in their entirety. [Background technology]

[0002] Biological arrays are among the wide range of tools used to detect and analyze molecules, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). In these applications, arrays are engineered to contain probes for nucleotide sequences present in the genes of humans and other organisms. In certain applications, for example, individual DNA and RNA probes can be attached to a geometric grid of locations (or randomly) on an array support. For example, a test sample from a human or tissue can be exposed to the grid such that complementary fragments hybridize to the probes at individual sites in the array. The array can then be tested by scanning a specific frequency of light across the sites, and the fluorescence of the sites to which the fragments are hybridized identifies which fragments are present in the sample.

[0003] Biological arrays can be used for gene sequencing. In general, gene sequencing involves determining the order of nucleotides or nucleic acids in a length of genetic material, such as a fragment of DNA or RNA. Longer sequences of base pairs are increasingly being analyzed, and the resulting sequence information can be used in a variety of bioinformatics techniques to logically match fragments together to reliably determine the sequence of extensive lengths of genetic material from which the fragments are derived. Automated computer-based testing of characteristic fragments has been developed and used in genome mapping, identification of genes and their functions, assessment of risk of certain conditions and disease states, and the like. Beyond these applications, biological arrays can be used for the detection and evaluation of a wide range of molecules, families of molecules, gene expression levels, single nucleotide polymorphisms, and genotypes. Summary of the Invention

[0004] A first embodiment disclosed herein is a switchable heteropolymer that includes a plurality of monomers that include a stimuli-responsive functional group, where the stimuli-responsive functional group is selected from the group consisting of a pH-responsive functional group, a temperature-responsive functional group, a sugar-responsive functional group, a nucleophile-responsive functional group, and a salt-responsive functional group.

[0005] The stimuli-responsive functional groups can undergo modification upon exposure to a given stimulus, which changes the polarity and / or conformation of the switchable heteropolymer.

[0006] In some embodiments, the switchable heteropolymer further comprises a primer grafted thereto.

[0007] In some embodiments, the stimulus-responsive functional group is not an azide group.

[0008] In some embodiments, the switchable heteropolymer comprises two or more different stimuli-responsive monomers that respond to the same or different stimuli.

[0009] In some embodiments, the switchable heteropolymer is a copolymer comprising a plurality of acrylamide monomers, each of which optionally comprises an azide group. In one of these embodiments, the acrylamide monomer is an azidoacetamidepentylacrylamide monomer, or a combination of an azidoacetamidepentylacrylamide monomer and a second acrylamide monomer.

[0010] In some embodiments, the switchable heteropolymer comprises a sugar monomer that optionally comprises an azide group.

[0011] In some embodiments, the pH-responsive functional group is selected from the group consisting of hydroxyl, 1,2-diol, 1,3-diol protected as an acetal, hemiacetal or ketal, tert-butyloxycarbonylamino group, 9H-fluoren-9-ylmethoxycarbonylamino group, amino group, carboxylate group, carboxylic acid group, sulfonate group and sulfonic acid group.

[0012] In other embodiments, the sugar-responsive functional group comprises a boronic acid group.

[0013] In yet another embodiment, the nucleophile-responsive functional group has the structure: [ka] wherein (a) Y is SO2 and Y' is CH2, or (b) Y and Y' are both C(O). In yet another embodiment, the salt-responsive functional group is a zwitterionic functional group that exhibits anti-polyelectrolyte behavior.

[0014] In some embodiments, the temperature responsive group comprises a thermally sensitive hydroxyl or amino protecting group.

[0015] It should be understood that any of the features of the switchable heteropolymers disclosed herein can be combined together in any desired manner and / or configuration.

[0016] Another aspect disclosed herein is a method for making a switchable heteropolymer, comprising copolymerizing a plurality of monomers that include a stimulus-responsive functional group with a plurality of second monomers.

[0017] In some embodiments, the second monomer is a sugar monomer or an acrylamide monomer that includes an azide group. In some embodiments, the acrylamide monomer includes an azide group. In some embodiments, the acrylamide monomer is azidoacetamidepentylacrylamide, or a combination of azidoacetamidepentylacrylamide and a second acrylamide monomer.

[0018] It should be understood that any features of the methods may be combined together in any desired manner. Moreover, it should be understood that any combination of features of the methods and / or switchable heteropolymers may be used together and / or combined with any of the embodiments disclosed herein.

[0019] Another embodiment disclosed herein is a flow cell, comprising a support and a switchable heteropolymer bound to the support. Any switchable heteropolymer disclosed herein may be used. In some embodiments, the flow cell further comprises a primer grafted to the switchable heteropolymer.

[0020] It should be understood that any features of the flow cell may be combined together in any desired manner. Moreover, it should be understood that any combination of features of the flow cell and / or method and / or switchable heteropolymer may be used together and / or combined with any of the embodiments disclosed herein.

[0021] Yet another aspect disclosed herein is a method of making a flow cell, comprising contacting a switchable heteropolymer with at least a portion of a flow cell support, thereby binding the switchable heteropolymer to the flow cell support.

[0022] In some embodiments, the method comprises grafting a primer onto a switchable heteropolymer bound on a support.

[0023] In another embodiment, the method comprises exposing a switchable heteropolymer bound to a flow cell support to a predetermined stimulus.

[0024] It should be understood that any features of the method may be combined together in any desired manner. Moreover, it should be understood that any combination of features of the flow cell and / or the method and / or the switchable heteropolymer may be used together and / or combined with any of the embodiments disclosed herein.

[0025] Yet another embodiment is a method of sequencing comprising grafting a primer onto a switchable heteropolymer on a flow cell support, exposing the switchable heteropolymer on the flow cell support to a predetermined stimulus thereby causing a change in the polarity and / or conformation of the switchable heteropolymer, hybridizing a nucleic acid template to the primer on the flow cell support, amplifying the nucleic acid template on the flow cell support to produce an amplified template, and detecting a signal when a labeled nucleotide binds to a complementary nucleotide of the amplified template.

[0026] It should be understood that any features of the method may be combined together in any desired manner. Moreover, it should be understood that any combination of features of the flow cell and / or the method and / or the switchable heteropolymer may be used together and / or combined with any of the embodiments disclosed herein.

[0027] Yet another embodiment disclosed herein is a heteropolymer comprising a monomer comprising a linking group reactive with a functional group attached to the primer and a monomer comprising a stimulus-responsive functional group, wherein the monomer comprising a stimulus-responsive functional group is selected from the group consisting of an acrylamide monomer comprising a terminal pH-responsive functional group; a vinyl or acrylate monomer comprising a terminal pH-responsive functional group selected from the group consisting of a hydroxyl having an acid labile protecting group, a hydroxyl having a base labile protecting group, an amino having an acid labile protecting group, an amino having a base labile protecting group, a sulfonate group, and a sulfonic acid group; a temperature-responsive N-substituted acrylamide; an acrylamide, acrylate, or vinyl monomer comprising a terminal sugar-responsive functional group; an acrylamide, acrylate, or vinyl monomer comprising a terminal nucleophile-responsive functional group; and an acrylamide, acrylate, or vinyl monomer comprising a terminal salt-responsive functional group.

[0028] In some embodiments, monomers containing stimuli-responsive functional groups undergo modification upon exposure to a given stimulus, where the modification changes the polarity and / or conformation of the switchable heteropolymer.

[0029] Some embodiments of this heteropolymer further include a primer grafted to the linking group.

[0030] In some embodiments, the linking group is selected from the group consisting of an azide group, an alkenyl group, an alkynyl group, an aldehyde group, a hydrazone group, a hydrazine group, a tetrazole group, a tetrazine group, and a thiol group.

[0031] In other embodiments, the linking group of the acrylamide monomer comprises an azide group. In some embodiments, the acrylamide monomer is an azidoacetamidopentylacrylamide monomer. In some embodiments, the heteropolymer further comprises a second acrylamide monomer.

[0032] In some embodiments, the monomer comprising a stimulus-responsive functional group is an acrylamide monomer comprising a terminal pH-responsive functional group, the terminal pH-responsive functional group being selected from the group consisting of a hydroxyl, a 1,2-diol, a 1,3-diol protected as an acetal, a hemiacetal or a ketal, a tert-butyloxycarbonylamino group, a 9H-fluoren-9-ylmethoxycarbonylamino group, an amino group, a carboxylate group, a carboxylic acid group, a sulfonate group and a sulfonic acid group.

[0033] In another embodiment, the monomer containing the stimuli-responsive functional group is an acrylamide, acrylate, or vinyl monomer containing a terminal sugar-responsive functional group, and the terminal sugar-responsive functional group contains a boronic acid group. In one embodiment, the monomer containing the stimuli-responsive functional group is 3-(acrylamido)phenylboronic acid.

[0034] In some embodiments, the monomer comprising a stimulus-responsive functional group is an acrylamide, acrylate, or vinyl monomer comprising a terminal nucleophile-responsive functional group, the terminal nucleophile-responsive functional group having the following structure: [ka] where (a) Y is SO2 and Y' is CH2, or (b) Y and Y' are both C(O).

[0035] In another embodiment, the monomer containing the stimuli-responsive functional group is an acrylamide, acrylate, or vinyl monomer containing a terminal salt-responsive functional group, which is a zwitterionic functional group that exhibits anti-polyelectrolyte behavior. In one embodiment, the monomer containing the stimuli-responsive functional group has one of the following structures: [ka] where A is O or NH, and R z is H or C 1-4 is alkyl, or [ka] Here, R z is H or C 1-4 It is an alkyl.

[0036] In some embodiments, the monomer comprising a stimulus-responsive functional group is a temperature-responsive N-substituted acrylamide, where the temperature-responsive substituted acrylamide comprises a thermally sensitive hydroxyl or amino protecting group.

[0037] In some embodiments, the monomer comprising a stimuli-responsive functional group is a temperature-responsive N-substituted acrylamide, and the temperature-responsive N-substituted acrylamide is N-isopropylacrylamide.

[0038] It should be understood that any of the features of the heteropolymer may be combined together in any desired manner. Moreover, it should be understood that any combination of features of the heteropolymer and / or flow cell and / or any of the methods may be used together in any desired manner and / or combined with any of the embodiments disclosed herein.

[0039] Another aspect disclosed herein is a heteropolymer having the structure: [ka] where n ranges from 19 to 500.

[0040] It should be understood that any of the features of the heteropolymer may be combined together in any desired manner. Moreover, it should be understood that any combination of features of the heteropolymer and / or flow cell and / or any of the methods may be used together in any desired manner and / or combined with any of the embodiments disclosed herein.

[0041] Yet another embodiment disclosed herein is a method for making a heteropolymer, comprising selecting a monomer containing a stimuli responsive functional group from the group consisting of an acrylamide monomer containing a terminal pH responsive functional group; a vinyl or acrylate monomer containing a terminal pH responsive functional group selected from the group consisting of hydroxyl with an acid labile protecting group, hydroxyl with a base labile protecting group, amino with an acid labile protecting group, amino with a base labile protecting group, a sulfonate group, and a sulfonic acid group; a temperature responsive N-substituted acrylamide; an acrylamide, acrylate, or vinyl monomer containing a terminal sugar responsive functional group; an acrylamide, acrylate, or vinyl monomer containing a terminal nucleophile responsive functional group; and an acrylamide, acrylate, or vinyl monomer containing a terminal salt responsive functional group; and copolymerizing the monomer containing the stimuli responsive functional group with an acrylamide monomer containing a linking group that reacts with the functional group attached to the primer.

[0042] In some embodiments, the acrylamide monomer having a linking group is an azidoacetamidopentylacrylamide monomer.

[0043] Some embodiments of this method include copolymerizing a monomer having a stimulus-responsive functional group and a monomer containing a linking group with a second acrylamide monomer.

[0044] It should be understood that any combination of the features of this method and / or flow cell and / or method and / or any heteropolymer may be used together and / or combined with any embodiment disclosed herein.

[0045] Features of examples of the present disclosure will become apparent by reference to the following detailed description and the drawings in which like reference numerals correspond to similar, though perhaps not identical, components. For purposes of brevity, reference numerals or features having a previously described function may or may not be described in conjunction with the other drawings in which they appear. [Brief description of the drawings]

[0046] [Figure 1] 1A to 1D are schematic cross-sectional views illustrating examples of the methods disclosed herein in conjunction with FIGS. 1E and 1F, or in conjunction with FIGS. 1G and 1H, respectively. [Diagram 2] 2A to 2D are schematic cross-sectional views illustrating another example of the method disclosed herein. [Diagram 3] 3A and 3B are graphs showing the error rate percentage (FIG. 3A) and quality metric percentage (FIG. 3B) of flow cells formed with comparative and example polymers disclosed herein. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0047] The singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise.

[0048] The terms comprising, including, containing and various forms of these terms are synonymous with each other and are equally broad in meaning.

[0049] The terms "top," "bottom," "lower," "upper," "above," and the like are used herein to describe the flow cell and / or various components of the flow cell. It is understood that these directional terms do not imply a particular orientation, but are used to indicate the relative orientation between the components. The use of directional terms should not be construed as limiting the embodiments disclosed herein to any particular orientation.

[0050] As used herein, "acetal group" refers to a functional group having the following connectivity: R2C(OR')2, where the R and R' groups are each organic fragments. Acetal groups include acetals, ketals, hemiacetals, and hemiketals. In acetals, one R group is H. In some embodiments, R' is C. 1-4 Alkyl, or two R's together form C 2-4 To form an alkylene, the acetal protecting group can be used to protect hydroxyl, 1,2-diol or 1,3-diol groups.

[0051] "Acrylate groups" include the salts, esters, and conjugate bases of acrylic acid and its derivatives (e.g., methacrylic acid). An acrylate ion has the molecular formula CH2=CHCOO - has.

[0052] "Acrylamide monomer" has the structure [ka] or a monomer having a substituted analogous structure thereof (e.g., methacrylamide or N-isopropylacrylamide). An example of a monomer containing an acrylamide group and an azide group is azidoacetamidepentylacrylamide: [ka] It is.

[0053] As used herein, "alkyl" refers to a straight or branched, fully saturated (i.e., containing no double or triple bonds) hydrocarbon chain. The alkyl group may have from 1 to 20 carbon atoms. Examples of alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tertiary butyl, pentyl, hexyl, and the like. By way of example, the designation "C1-4 alkyl" means that there are from 1 to 4 carbon atoms in the alkyl chain, i.e., the alkyl chain is selected from the group consisting of methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, sec-butyl, and t-butyl.

[0054] As used herein, "alkenyl" refers to a straight or branched hydrocarbon chain containing one or more double bonds. Alkenyl groups can have from 2 to 20 carbon atoms. Alkenyl groups include ethenyl, propenyl, butenyl, pentenyl, hexenyl, and the like.

[0055] As used herein, "alkyne" or "alkynyl" refers to a straight or branched hydrocarbon chain containing one or more triple bonds. Alkynyl groups can have from 2 to 20 carbon atoms.

[0056] As used herein, "aryl" refers to an aromatic ring or ring system (i.e., two or more fused rings sharing two adjacent carbon atoms) containing one carbon atom in the ring backbone. When aryl is a ring system, all rings in the system are aromatic. Aryl groups can have from 6 to 18 carbon atoms. Examples of aryl groups include phenyl, naphthyl, azulenyl, and anthracenyl.

[0057] As used herein, the term "attached" refers to the state of two things being linked, fixed, attached, connected, or bound to one another, either covalently or non-covalently (e.g., by hydrogen bonds, ionic bonds, van der Waals forces, hydrophilic and hydrophobic interactions). For example, a nucleic acid can be attached to a functionalized polymer by covalent or non-covalent bonds.

[0058] An "azide" or "azido" functional group refers to --N3.

[0059] As used herein, a "bonding region" refers to a region on a substrate that is bonded to another material, which may be, by way of example, a spacer layer, a lid, another substrate, etc., or a combination thereof (e.g., a spacer layer and a lid). The bond formed in the bonding region may be a chemical bond (as described above) or a mechanical bond.

[0060] "tert-Butyloxycarbonyl group" (Boc) is [ka] The term "butyloxycarbonyloxy group" refers to -OCO2tBu.

[0061] As used herein, "carbocyclyl" refers to a non-aromatic cyclic ring or ring system containing only carbon atoms in the ring system backbone. When a carbocyclyl is a ring system, two or more rings can be fused, bridged, or spiro-linked together. A carbocyclyl can have any degree of saturation, as long as at least one ring of the ring system is not aromatic. Thus, carbocyclyl includes cycloalkyl, cycloalkenyl, and cycloalkynyl. A carbocyclyl group can have 3 to 20 carbon atoms. Examples of carbocyclyl rings include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl, 2,3-dihydro-indene, bicyclo[2.2.2]octanyl, adamantyl, and spiro[4.4]nonanyl.

[0062] As used herein, the term "carboxylic acid" or "carboxyl" refers to --COOH.

[0063] As used herein, "cycloalkylene" means a fully saturated carbocyclyl ring or ring system attached to the remainder of the molecule through two points of attachment.

[0064] As used herein, "cycloalkenyl" or "cycloalkene" refers to a carbocyclyl ring or ring system having at least one double bond, and no aromatic rings are present in the ring system. Examples include cyclohexenyl or cyclohexane, and norbornenyl or norbornene. As further used herein, "heterocycloalkenyl" or "heterocycloalkene" refers to a carbocyclyl ring or ring system containing at least one heteroatom in the ring backbone and at least one double bond, and no aromatic rings are present in the ring system.

[0065] As used herein, "cycloalkynyl" or "cycloalkyne" refers to a carbocyclyl ring or ring system having at least one triple bond, and no aromatic rings in the ring system. An example is cyclooctyne. Another example is bicyclononyne. As further used herein, "heterocycloalkynyl" or "heterocycloalkyne" refers to a carbocyclyl ring or ring system containing at least one heteroatom in the ring backbone and at least one double bond, and no aromatic rings in the ring system.

[0066] The term "depositing" as used herein refers to any suitable application technique, which may be manual or automated, that results in the modification of surface properties. In general, depositing may be performed using deposition techniques, coating techniques, grafting techniques, and the like. Some specific examples include chemical vapor deposition (CVD), spray coating (e.g., ultrasonic spray coating), spin coating, dunk or dip coating, doctor blade coating, paddle dispensing, flow-through coating, aerosol printing, screen printing, microcontact printing, inkjet printing, and the like.

[0067] As used herein, the term "depression" refers to a fractional concave feature in a patterned support having a surface opening completely surrounded by an interstitial region of the patterned support surface. The depression can have a variety of shapes in the surface opening, such as, for example, a circle, an ellipse, a square, a polygon, a star (with any number of vertices). The cross section of the depression taken perpendicular to the surface can be curved, square, polygonal, hyperbolic, conical, angular, etc. As an example, the depression can be a well. As further used herein, "functionalized depression" refers to a fractional concave feature to which the polymers and primers disclosed herein are attached.

[0068] The term "each," when used in reference to a collection of items, is intended to identify each individual item in the collection, but does not necessarily refer to every item in the collection. Exceptions may occur where explicit disclosure or context clearly dictates otherwise.

[0069] As used herein, the term "flow cell" is intended to mean a vessel having a chamber (i.e., a flow channel) in which a reaction can be carried out, an inlet for delivering a reagent to the chamber, and an outlet for removing the reagent from the chamber. In some instances, the chamber allows for detection of a reaction or signal occurring within the chamber. For example, the chamber can include one or more transparent surfaces that allow for optical detection of arrays, optically labeled molecules, etc. within the chamber.

[0070] As used herein, a "flow channel" or "flow channel region" may be a region provided between two joined components that can selectively receive a liquid sample. In some examples, a flow channel may be provided between a patterned support and a lid, thereby fluidly connecting with one or more depressions provided in the patterned support. In other examples, a flow channel may be defined between a non-patterned support and a lid.

[0071] The "fluorenylmethyloxycarbonyl" group (Fmoc) is a base-labile protecting group and has the following structure: [ka]

[0072] As used herein, "heteroaryl" refers to an aromatic ring or ring system (e.g., two or more fused rings sharing two adjacent atoms) that contains one or more heteroatoms, i.e., elements other than carbon, including but not limited to nitrogen, oxygen, and sulfur, in the ring backbone. When a heteroaryl is a ring system, all rings in the system are aromatic. Heteroaryl groups can have from 5 to 18 ring members.

[0073] As used herein, "heterocyclyl" refers to a non-aromatic ring or ring system that contains at least one heteroatom in the ring backbone. Heterocyclyls may be bonded together in a fused, bridged, or spiro-bonded manner. Heterocyclyls may have any degree of saturation, so long as at least one ring in the ring system is not aromatic. In the ring system, the heteroatoms may be present in either the non-aromatic or aromatic ring. Heterocyclyl groups may have 3 to 20 ring members (i.e., the number of atoms that form the ring backbone, including carbon atoms and heteroatoms). In some examples, the heteroatom is O, N, or S.

[0074] As used herein, the term "hydrazine" or "hydrazyl" refers to an optionally substituted -NHNH2 group.

[0075] As used herein, the term "hydrazone" or "hydrazonyl" refers to [ka] R a and R b are each independently selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-7 carbocyclyl, C 6-10 aryl, 5-10 membered heteroaryl, and 5-10 membered heterocyclyl, as defined herein.

[0076] As used herein, "hydroxy" or "hydroxyl" refers to an --OH group.

[0077] As used herein, the term "interstitial region" refers to a region on a support or surface that partitions a depression. The ring backbone contains one or more heteroatoms, i.e., elements other than carbon, including but not limited to nitrogen, oxygen, and sulfur. When a heteroaryl is a ring system, all rings in the system are aromatic. For example, the interstitial region can separate one function of the array from another function of the array. The two functions that are separated from each other can be separate, i.e., lack physical contact with each other. In another example, the interstitial region can separate a first portion of a feature from a second portion of the feature. In many examples, the interstitial region is continuous, but the features are separate, such as in the case of multiple wells on an otherwise continuous surface. The separation provided by the interstitial region can be partial or complete separation. The interstitial region can have a surface material that is different from the surface material of the features on the surface. For example, the features of the array can have an amount or concentration of coating layers and primers that exceeds the amount or concentration present in the interstitial region. In some instances, the coating layer and primer may not be present in the interstitial regions.

[0078] As used herein, a "nucleotide" comprises a nitrogenous heterocyclic base, a sugar, and one or more phosphate groups. A nucleotide is a monomeric unit of a nucleobase sequence. In RNA, the sugar is ribose, and in DNA, the sugar is deoxyribose, i.e., a sugar lacking the hydroxyl group present at the 2' position of the ribose. The nitrogenous heterocyclic base (i.e., the nucleobase) can be a purine base or a pyrimidine base. Purine bases include adenine (A) and guanine (G) and modified derivatives or analogs thereof. Pyrimidine bases include cytosine (C), thymine (T) and uracil (U) and modified derivatives or analogs thereof. The C-1 atom of the deoxyribose is attached to the N-1 of the pyrimidine or the N-9 of the purine.

[0079] As used herein, "plasma ashing" refers to a process of removing organics from a substrate by oxygen plasma. Products resulting from plasma ashing may be removed with a vacuum pump / system. Plasma ashing may activate the substrate by introducing reactive hydroxyl groups.

[0080] A "heteropolymer" or "heteropolymer coating layer" as referred to herein is intended to mean a large molecule of at least two different repeating subunits (monomers), where one of the repeating subunits (monomers) contains a stimuli-responsive functional group.

[0081] As used herein, a "primer" is defined as a single-stranded nucleic acid sequence (e.g., single-stranded DNA or single-stranded RNA) that serves as the initiation point for DNA or RNA synthesis. The 5' end of the primer may be modified to allow for a coupling reaction with a functionalized polymer layer. The length of the primer can be any number of bases long and can include a variety of non-natural nucleotides. In one example, the primer is a short strand ranging from 20 to 40 bases.

[0082] As used herein, the terms "silane" and "silane derivatives" refer to organic or inorganic compounds containing one or more silicon atoms. Examples of inorganic silane compounds are SiH4 or halogenated SiH4 where the hydrogen atoms are replaced by one or more halogen atoms. Examples of organosilane compounds are XR B -Si(OR C )3, where R-Si is an organic linking group, X is a functional group such as amino, vinyl, methacrylate, epoxy, sulfur, alkyl, alkenyl, or alkynyl; R B is a spacer, e.g. -(CH2) n -, where n is 0 to 1000; R C is selected from hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted 5-10 membered heteroaryl and optionally substituted 5-10 membered heterocyclyl, as defined herein. As used herein, the terms "silane" and "silane derivative" can include mixtures of different silane and / or silane derivative compounds.

[0083] As used herein, a "spacer layer" refers to a material that bonds two elements together. In some instances, the spacer layer refers to a radiation absorbing material that aids in bonding or that can be in contact with a radiation absorbing material that aids in bonding.

[0084] As used herein, "stimulus-responsive functional group" refers to a portion of an atom and / or bond in a polymer that can change its state in response to a stimulus. The stimuli-responsive functional group can be pH-responsive, temperature-responsive, sugar-responsive, nucleophile-responsive, or salt-responsive. Specific examples of each stimuli-responsive functional group are further described below.

[0085] The term flow cell "support" or "substrate" refers to a support or substrate to which surface chemistry can be applied. The term "patterned substrate" refers to a support having or on which depressions are defined. The term "unpatterned substrate" refers to a substantially planar support. The substrate may also be referred to herein as a "support", "patterned support", or "unpatterned support". The support may be a wafer, a panel, a rectangular sheet, a die, or any other suitable configuration. The support is generally rigid and insoluble in aqueous liquids. The support may be inert to the chemicals used to modify the depressions. For example, the support may be inert to the chemicals used to bond the primer to the polymer coating layer to form the polymer coating layer. Examples of suitable supports are epoxy siloxanes, glass and modified or functionalized glass, polyhedral oligomeric silsequioxanes (POSS) and their derivatives, plastics (acrylics, polystyrene and copolymers of styrene and other materials, polypropylene, polyethylene, polybutylene, polyurethane, polytetrafluoroethylene (TEFLON from Chemours), and the like. (R) Other materials that may be used include cyclic olefins / cyclic olefin polymers (COPs) (such as Zeon's ZEONOR®), polyimides, nylons, ceramics / ceramic oxides, silica, fused silica, or silica-based materials, aluminum silicates, silicon and modified silicon (e.g., boron-doped p+ silicon), silicon nitride (Si3N4), silicon oxide (SiO2), tantalum pentoxide (TaO5) or other tantalum oxides (TaO x ), hafnium oxide (HaO2), carbon, metals, inorganic glasses, etc. The support may also be glass or silicon, or a silicon-based polymer such as a POSS material, optionally having a coating layer of tantalum oxide or another ceramic oxide on the surface.

[0086] The term "surface chemistry" as used herein refers to chemically and / or biologically active moieties incorporated into the channels of a flow cell. Examples of surface chemistry disclosed herein include a polymeric coating layer attached to at least a portion of the surface of the support, and a primer attached to at least a portion of the polymeric coating layer.

[0087] A "thiol" functional group refers to -SH.

[0088] As used herein, the terms "tetrazine" and "tetrazinyl" refer to a six-membered heteroaryl group containing four nitrogen atoms. Tetrazine can be optionally substituted.

[0089] As used herein, "tetrazole" refers to a 5-membered heteroaryl group containing four nitrogen atoms. The tetrazole may be optionally substituted.

[0090] An example of a flow cell disclosed herein includes a support, a polymer attached to the support, and a primer grafted to the polymer. Examples of flow cells are shown in Figures 1F, 1H, and 3D and will be further described herein. Next, various examples of polymers that are attached to the flow cell support will be described.

[0091] References throughout this specification to "one example," "another example," "an example," etc. mean particular elements (e.g., features, structures, and / or characteristics) described in connection with that example that are included in at least one example described herein and may or may not be present in other examples. Further, unless the context clearly dictates otherwise, it should be understood that elements described in any example can be combined in any suitable manner in the various examples.

[0092] Ranges provided herein should be understood to include the stated range and any value or subrange within the stated range, as if that range and value or subrange were expressly stated herein. For example, a range of about 400 nm to about 1 μm (1000 nm) should be interpreted not only to include the expressly stated limits of about 400 nm to about 1 μm, but also to include individual values ​​such as about 708 nm, about 945.5 nm, etc., and subranges such as about 425 nm to about 825 nm, about 550 nm to about 940 nm, etc. Furthermore, "about" and / or "substantially" are used to describe values ​​and are meant to include slight variations (up to + / - 10%) from the stated values.

[0093] The heteropolymers described herein include stimuli-responsive functional groups that can undergo modification when exposed to a given stimulus, the modification changing the polarity and / or conformation of the heteropolymer. Thus, examples of heteropolymers disclosed herein can be switchable. Switchable heteropolymers can transition from an initial state to a second switched state when exposed to a particular stimulus. Examples of switching include polarity changes such as hydrophobic to hydrophilic, increased hydrophilicity, hydrophilic to hydrophobic, increased hydrophobicity, neutral to charged, charged to neutral, anionic to neutral, cationic to neutral, neutral to anionic, neutral to cationic, and neutral to neutral with increased hydrophilicity. Examples of switching also include conformational changes such as swelling, folded to extended, extended to folded (e.g., with anti-polyelectrolyte behavior), and coil-globule formation. A given stimuli-responsive group may provide multiple switching effects. In some embodiments, the switch is irreversible, while in other instances, the switch is reversible under different chemical or thermal conditions.

[0094] The stimuli-responsive functional group exhibits an initial state when the switchable heteropolymer is applied to the flow cell support. In some embodiments, the initial state is compatible with the hydrophobic nature of the flow cell support, which facilitates the manufacture and handling of the flow cell. For example, the initial state (e.g., hydrophobic) can improve the adhesion and coating uniformity of the heteropolymer to the hydrophobic flow cell support, while the switching state can be more compatible with the use of the flow cell, such as sequencing operations.

[0095] Thus, in some embodiments, the switched state may be a solution conformation that provides improved performance in some applications, including sequencing operations. Prior to sequencing operations, the stimuli-responsive polymers disclosed herein may be exposed to a predetermined stimulus. Upon exposure to the stimulus, the switchable heteropolymer changes polarity and / or conformation due to the effect of the predetermined stimulus on the stimuli-responsive functional groups. The switched state heteropolymer may provide a low-fouling surface that may reduce non-specific adsorption of proteins and improve sequence metrics (e.g., base or first cycle intensity, quality score, error rate, etc.).

[0096] One of ordinary skill in the art will recognize that any of the heteropolymers described herein may be random, block, linear, and / or branched copolymers containing two or more repeating monomeric units in any order or configuration, and may be linear, crosslinked or branched, or combinations thereof.

[0097] In some examples, the stimuli-responsive functional group is a pH-responsive functional group.In such an embodiment, the switchable heteropolymer is a copolymer that includes a plurality of monomers that contain pH-responsive functional groups.The plurality of monomers may each have the same pH-responsive functional group or different pH-responsive functional groups that respond to the same pH condition.In some embodiments, the heteropolymer is a copolymer of a plurality of acrylamide monomers.A single type of acrylamide monomer can be used, or two or more different acrylamide monomers can be used.

[0098] In some embodiments, the pH-responsive functional group is converted to a substituent with increased or decreased polarity (e.g., increased hydrophilicity or increased hydrophobicity) upon exposure to acidic or basic pH conditions. In some embodiments, the pH-responsive functional group is neutral and becomes charged upon exposure to a stimulus. In some embodiments, the pH-responsive functional group is charged and becomes neutral upon exposure to a stimulus. In some embodiments, the pH-responsive functional group is neutral and is converted to a different group that is neutral but more polar upon exposure to a stimulus.

[0099] In some embodiments, the pH-responsive functional group is a hydroxyl with an acid labile protecting group (which switches to a more hydrophilic free hydroxyl when exposed to acidic / low pH conditions), a hydroxyl with a base labile protecting group (which switches to a more hydrophilic free hydroxyl when exposed to basic / high pH conditions), an amino with an acid labile protecting group (which switches to a more hydrophilic free amino group when exposed to acidic / low pH conditions), an amino with a base labile protecting group (which switches to a more hydrophilic free amino group when exposed to basic / high pH conditions), an amino group (which switches to an ammonium ion when exposed to acidic / low pH conditions), a carboxylate (-CO2 - ) groups (which switch to neutral carboxylic acids when exposed to acidic / low pH conditions), carboxylic acid groups (which switch to charged, more hydrophilic carboxylates when exposed to basic / high pH conditions), sulfonates (-SO3 -) groups (which switch to neutral sulfonic acids when exposed to acidic / low pH conditions), or sulfonic acid groups (which switch to charged, more hydrophilic sulfonates when exposed to basic / high pH conditions).

[0100] An exemplary switchable heteropolymer includes monomers of the following structure: [ka] Where: X is -O-PG, -NH-PG, -NR a R b , -SO3H, -SO3 - , -CO2H, and -CO2 - The pH responsive functional group is selected from the group consisting of: PG is an acid- or base-labile protecting group (eg, Boc, Fmoc, or acetal). R a and R b are each independently H or C1-4 alkyl. In some embodiments, X is -O-Boc, -NHBoc, -NHFmoc, -NH2, -NHCH3, or -N(CH3)2. In some embodiments, X is SO3H, -SO3 - , -CO2H, or -CO2 - In some embodiments, R a and R b are both methyl. In some embodiments, R z is H or methyl. In some embodiments, the monomer has the structure: [ka]

[0101] Thus, in some embodiments, the pH-responsive functional group is a 1,2-diol or 1,3-diol protected as a hydroxyl, acetal, hemiacetal, or ketal (which switches to a more polar / hydrophilic diol upon exposure to acidic / low pH conditions), a tert-butyloxycarbonylamino group, a 9H-fluoren-9-ylmethoxycarbonylamino group, an amino group, a carboxylate (-CO2 - ) group, carboxylic acid group, sulfonate (-SO3 - ) group, or a sulfonic acid group.

[0102] The tert-butoxycarbonylamino group may be in a hydrophobic (or less hydrophilic) state and may transition to a hydrophilic state (e.g., an amino group) when exposed to a low (acidic) pH (e.g., having a pH of less than 7). The tert-butoxycarbonylamino group may be attached to an acrylamide monomer or an acrylate monomer. Examples of tert-butoxycarbonylamino group-containing monomers include N-(tert-butoxycarbonyl-aminoethyl) methacrylamide, N-(tert-butoxycarbonyl-aminopropyl) methacrylamide, and (2-tert-butoxycarbonyl-amino)ethyl methacrylate.

[0103] The 9H-fluoren-9-yl methoxycarbonyl amino group may be in a hydrophobic (or less hydrophilic) state and may transition to a hydrophilic state (e.g., an amino group) upon exposure to a low (acidic) pH (e.g., having a pH less than 7). The 9H-fluoren-9-yl methoxycarbonyl amino group may be attached to an acrylamide monomer, or an acrylate monomer, or a vinyl monomer. Examples of 9H-fluoren-9-yl methoxycarbonyl group-containing monomers include the following: [ka]

[0104] The amino group may be in a neutral state and may transition to a charged (and more hydrophilic) state (cationic) when exposed to a low (acidic) pH (e.g., having a pH of less than 7). For example, the amino group of the synthesized polymer may be protonated, resulting in a cationic charge around the polymer backbone. In the synthesized polymer, the amino group may be attached to an acrylamide or acrylate monomer, or a vinyl monomer. Examples of amino group-containing monomers include 2-(dimethylamino)ethyl methacrylate, 2-(N,N-dimethylamino)ethyl acrylate, N-[3-(N,N-dimethylamino)propyl]acrylamide, N-[2-(N,N-dimethylamino)ethyl]methacrylamide, and N-[3-(N,N-dimethylamino)propyl]methacrylamide.

[0105] The acetal group may transition to a more hydrophilic state (hydroxyl or diol) than its initial state when exposed to low (acidic) pH (e.g., pH less than 7). The acetal group may be attached to azide-functionalized hyaluronic acid (HA-N3). Since HA-N3 has limited solubility in organic solvents, it may be converted to a tetrabutylammonium salt using an acidic ion exchange resin prior to the acetalization reaction. The acetalization reaction may be carried out by reacting 2-methoxypropene and pyridinium p-toluenesulfonate with the HA-N3 salt dissolved in dimethylsulfoxide (DMSO) at room temperature.

[0106] In some embodiments, the switchable heteropolymer further comprises an azide-containing acrylamide monomer. [ka] Including, in some cases [ka] In some embodiments, the switchable heteropolymer comprises the structure [ka] where R z are each independently H or C 1-4 In some embodiments, X is -O-Boc, -NHBoc, -NHFmoc, -NH2, -NHCH3, or -N(CH3)2. In some embodiments, X is -NHBoc. In some embodiments, X is SO3H, -SO3 - , -CO2H, or -CO2 - In some embodiments, R a and R b and R are both methyl groups. z are each independently H or methyl.

[0107] In some examples, the switchable heteropolymer comprises two pH-responsive acetal functional groups (such as one ketal group and one hemiketal group) and a carboxylic acid group. An exemplary heteropolymer has the following structure: [ka] where n ranges from 10 to 500. The acid-labile acetal groups protect multiple alcohols on the polar dextran backbone of azide-modified hyaluronic acid (HA-N3), changing the properties of the heteropolymer from hydrophilic to hydrophobic. Heteropolymers in a more hydrophobic initial state are easier to handle and process, and may allow the heteropolymer to be more effectively coated or attached to hydrophobic supports disclosed herein (e.g., norbornene-functionalized glass or POSS substrates).

[0108] In some examples, the stimuli-responsive functional group is a temperature-responsive functional group. In such an embodiment, the switchable heteropolymer is a copolymer comprising a plurality of monomers that contain a temperature-responsive functional group. Each of the plurality of monomers may have the same or different temperature-responsive functional groups that respond to the same temperature condition. In some embodiments, the heteropolymer is a copolymer with a plurality of acrylamide monomers. A single type of acrylamide monomer may be used, or two or more different acrylamide monomers may be used.

[0109] Temperature-responsive functional groups are those that can be converted to more or less polar functional groups or can cause conformational changes in the polymer with temperature changes. For example, temperature-responsive groups include heat-sensitive hydroxyl or amino protecting groups (such as Boc or Fmoc groups) that are removed when the heteropolymer is exposed to heat (switching from a neutral initial state to a neutral state with increased hydrophilicity). Various Boc and Fmoc protected monomers, including acrylic monomers, may be used. In another example, the temperature-responsive functional group allows the polymer to exist in an extended initial state that is neutral and relatively hydrophilic at room temperature, and then switch the heteropolymer to a folded state that is neutral and relatively hydrophobic at elevated temperatures (such as 32° C. or higher). Another example of this is coil-sphere switching, which can be exhibited by, for example, poly(N-isopropylacrylamide). It should be understood that primers grafted to the switchable heteropolymer may slightly alter this behavior. In some embodiments, the polymeric material thermally undergoes a coil-to-globule transition. In some embodiments, the temperature-responsive functional group is part of a polymer that is an ionizable, thermosensitive gel. In some embodiments, the monomer that includes the temperature-responsive functional group is H2C=C(H or methyl)-C(O)NR c R d where R is an N-substituted acrylamide such as c is H and R d is branch C 3-6In some embodiments, the monomer comprising the temperature responsive functional group is N-isopropylacrylamide, optionally in the blocked state of poly(N-isopropylacrylamide).

[0110] In one example, the heteropolymer comprises a temperature-responsive functional group monomer and an acrylamide monomer. In some examples, the acrylamide monomer is selected from the group consisting of azidoacetamidepentylacrylamide monomer and a combination of an acrylamide monomer and an azidoacetamidepentylacrylamide monomer, as described above. In some embodiments, the switchable heteropolymer further comprises an azide-containing acrylamide monomer. In some embodiments, the switchable heteropolymer comprises: [ka] In some embodiments, the switchable heteropolymer comprises the following structure: [ka] Here, R z are each independently H or C 1-4 It is an alkyl.

[0111] In another embodiment, the stimuli-responsive functional group is a sugar-responsive functional group, which is a hydrophilic substituent that reacts with a diol reagent to form an anionic functional group. In some embodiments, the diol is an organic diol, or a sugar, or glucose. In some embodiments, the sugar-responsive functional group comprises a boronic acid, such as an alkylboronic acid or an arylboronic acid. The boronic acid functional group may be in a charge-neutral initial state (and may be relatively hydrophobic) and may transition to a negatively charged (anionic) state (which may be more hydrophilic than the charge-neutral state) upon exposure to a sugar solution. Boronic acids are capable of reacting with sugars to form boronate esters and reversibly swell with the influx of water. This may be desirable during sequencing operations.

[0112] The boronic acid functional group may be attached to an acrylamide monomer, or an acrylate monomer, or a vinyl monomer. In one example, a monomer containing a sugar-responsive functional group has the following structure: [ka] An example of a boronic acid group-containing monomer includes 3-(acrylamido)phenylboronic acid. In some embodiments, the switchable heteropolymer further comprises an acrylamide monomer. In some embodiments, the acrylamide monomer is an azide-containing acrylamide monomer. In some embodiments, the switchable heteropolymer comprises: [ka] In some embodiments, the heteropolymer has the following structure: [ka] Here, R z are each independently H or C 1-4 It is an alkyl.

[0113] In some examples, the stimuli-responsive functional group is a nucleophile-responsive functional group. A nucleophile-responsive functional group is a group that is susceptible to attack by a nucleophile, resulting in a structural change that results in a polar and / or conformational change as described herein. In some embodiments, the switchable heteropolymer is a copolymer that includes a plurality of monomers that include a nucleophile-responsive functional group. Each of the plurality of monomers may have the same nucleophile-responsive functional group or different nucleophile-responsive functional groups that respond to the same nucleophile. In some embodiments, the heteropolymer is a copolymer of a monomer that includes a nucleophile-responsive functional group and one or more acrylamide monomers. A single type of acrylamide monomer may be used, or two or more different acrylamide monomers may be used. In some examples, the nucleophile-responsive functional group is a cyclic sulfonate ester (such as a sultone ring) or a cyclic anhydride (such as succinic anhydride) that can undergo a ring-opening reaction upon exposure to a nucleophile, optionally under basic (high pH) conditions, such as pH 9 or higher.

[0114] In some embodiments, the nucleophile-responsive functional group has the following structure: [ka] wherein (a) Y is SO and Y' is CH, or (b) Y and Y' are both C(O). In other embodiments, the nucleophile-responsive functional group is [ka] Suitable nucleophiles include primary alkyl amines and alkyl alcohols. Examples of sultone ring-opening groups and their ring-opening reactions are: [ka] where M is H or a monovalent cation (sodium or potassium cation). The sultone ring-opening group may be in a hydrophobic (or less hydrophilic) state and upon exposure to a high (basic) pH, the ring-opening reaction may proceed to transition to a (more) hydrophilic state. The functional group after the ring-opening reaction may also be anionic and therefore in a charged state.

[0115] In some embodiments, the monomer containing a nucleophile-responsive functional group is: [ka] In a particular example, the monomer is: [ka]

[0116] In some embodiments, the nucleophile-responsive functional group can be attached to an acrylamide monomer, or an acrylate monomer, or a vinyl monomer. In some embodiments, the switchable heteropolymer further comprises an acrylamide monomer. In some examples, the acrylamide monomer is an azide-containing acrylamide monomer. In some embodiments, the switchable heteropolymer comprises: [ka]

[0117] In some embodiments, the heteropolymer has the following structure: [ka] Here, R z are each independently H or C 1-4 It is an alkyl.

[0118] In some embodiments, the stimuli-responsive functional group is a salt-responsive functional group. In some examples, the salt-responsive functional group is a zwitterionic functional group that exhibits anti-polyelectrolyte behavior, where the zwitterionic functional group switches from a folded state to an extended state when exposed to salt. The salt-responsive functional group is a zwitterionic functional group that has anti-polyelectrolyte behavior. As used herein, "anti-polyelectrolyte behavior" means that a monomer that includes a zwitterionic functional group switches from a folded state to an extended state when exposed to salt (i.e., the monomer is more soluble in salt water than in pure water). Thus, the salt-responsive functional group can be in a folded state (e.g., a state in which the polymer chain is globular) and can transition to an extended state (i.e., a state in which the polymer chain is extended) when exposed to a salt solution. The effect of the local salt counterion changes the conformation of the polymer chain that includes the salt-responsive functional group. In one example, the monomer containing the zwitterionic functional group is selected from the group consisting of N-(2-methacryloyloxy)ethyl-N,N-dimethylammoniopropanesulfonate and N-(3-methacryloylimino)propyl-N,N-dimethylammoniopropanesulfonate.

[0119] In some examples, the salt-responsive functional group is -NMe3 + Exemplary monomers are: [ka] Here, R z are each independently H or C 1-4 It is an alkyl. When combined with anionic counterparts (present in salt solutions), these charged materials may exhibit anti-polyelectrolyte behavior. Examples of suitable anionic counterparts include carboxylates, sulfonates, citrates, phosphates, etc.

[0120] The salt-responsive functional group may be attached to an acrylamide monomer, or an acrylate monomer, or a vinyl monomer. In some embodiments, the switchable heteropolymer further comprises an acrylamide monomer. In some examples, the acrylamide monomer is an azide-containing acrylamide monomer. In some embodiments, the switchable heteropolymer comprises: [ka] In some embodiments, the heteropolymer has the following structure: [ka] Here, R z are each independently H or C 1-4 It is an alkyl.

[0121] In one example, the flow cell includes a support and a switchable heteropolymer bound to the support, and the stimuli-responsive functional group is selected from the group consisting of a pH-responsive functional group, a temperature-responsive functional group, a sugar-responsive functional group, a nucleophile-responsive functional group, and a salt-responsive functional group. The stimuli-responsive functional group can undergo modification upon exposure to a predetermined stimulus, and the modification changes the polarity and / or conformation of the switchable heteropolymer.

[0122] In some embodiments, the flow cell support is a patterned substrate comprising depressions separated by interstitial regions and the heteropolymer is present within the depressions, hi other embodiments, the support is an unpatterned substrate having a flow channel region and a binding region and the heteropolymer is bound to the flow channel region.

[0123] In some embodiments, the flow cell further comprises a primer grafted to the switchable heteropolymer.

[0124] In an example of this embodiment of the flow cell, the surface of the support is functionalized with a silane or silane derivative, and the heteropolymer is bound to the silane or silane derivative. In some examples, the silane or silane derivative comprises an unsaturated moiety that can react with the functional group of the functionalized polymer layer. As used herein, the term "unsaturated moiety" refers to a chemical group that comprises a cycloalkene, cycloalkyne, heterocycloalkene, heterocycloalkyne, or variant that may be optionally substituted and comprises at least one double bond or one triple bond. The unsaturated moiety can be monovalent or divalent. When the unsaturated moiety is monovalent, cycloalkene, cycloalkyne, heterocycloalkene, and heterocycloalkyne are used interchangeably with cycloalkenyl, cycloalkynyl, heterocycloalkenyl, and heterocycloalkynyl, respectively. When the unsaturated moiety is divalent, cycloalkene, cycloalkyne, heterocycloalkene, and heterocycloalkyne are used interchangeably with cycloalkenylene, cycloalkynylene, heterocycloalkenylene, and heterocycloalkynylene, respectively.

[0125] The unsaturated moiety can be directly attached to the silicon atom of the silane or silane derivative, or indirectly attached via a linker. Examples of suitable linkers include optionally substituted alkylenes (e.g., divalent saturated aliphatic radicals that are considered to be derived from an alkene by opening a double bond, or from an alkane by removing two hydrogen atoms from different carbon atoms, such as ethylene), substituted polyethylene glycols, and the like.

[0126] The heteropolymers disclosed herein are composed of at least two different monomers. One of the monomers comprises a stimuli-responsive functional group. In some embodiments, the other monomer comprises a binding group that is reacted with the flow cell support and / or the primer to which the heteropolymer is bound. This other binding group may also be capable of binding to the support, or the other monomer may comprise a second (different) binding group that can be bound to the support. It should be understood that the polymers disclosed herein may also comprise one or more other monomers that do not interfere with the respective functions of the stimuli-responsive functional group and the binding group.

[0127] In any of the examples of the polymers disclosed herein, the linking group is selected from the group consisting of azide, amino, alkenyl (including cycloalkenyl or heterocycloalkenyl groups), alkynyl (including cycloalkynyl or heterocycloalkynyl groups), aldehyde, hydrazone, hydrazine, carboxyl, hydroxy, tetrazole, tetrazine, and thiol.

[0128] The linking group may be capable of reacting with a functional group attached to the 5' end of the primer. For example, a bicyclo[6.1.0]non-4-yne (BCN)-terminated primer may be captured by an azide linking group of a polymer via strain-promoted, uncatalyzed click chemistry. As another example, an alkyne-terminated primer may be captured by an azide linking group of a polymer via copper-catalyzed click chemistry. As yet another example, a norbornene-terminated primer may undergo uncatalyzed ring strain-promoted click reaction with a tetrazine linking group of a polymer. It should be understood that other coupling chemistries may be used to attach a primer to a linking group, including, for example, Staudinger ligation, strain-promoted reaction, and photo-click cycloaddition.

[0129] Other examples of terminal primers that can be used include tetrazine terminated primers, azide terminated primers, amino terminated primers, epoxy or glycidyl terminated primers, thiophosphate terminated primers, thiol terminated primers, aldehyde terminated primers, hydrazine terminated primers, and triazolinedione terminated primers.

[0130] In one example, the linking group is attached to an acrylamide monomer. One example of a monomer that includes a linking group is azidoacetamidopentylacrylamide.

[0131] In one example method, applying the polymer coating layer to the flow cell includes flow-through deposition, chemical vapor deposition, dip coating, dunk coating, spin coating, spray coating, paddle dispensing, ultrasonic spray coating, doctor blade coating, aerosol printing, screen printing, microcontact printing, or inkjet printing.

[0132] In one example of the method, the flow cell support is a patterned flow cell support including recesses separated by interstitial regions, and the method further includes, prior to applying the polymer coating layer, binding a silane or silane derivative to a surface of the patterned flow cell support, thereby forming silanized recesses and silanized interstitial regions, applying the polymer coating layer to the silanized recesses and silanized interstitial regions, and removing (e.g., polishing) the polymer coating layer from the silanized interstitial regions.

[0133] In one example of the method, exposing the polymer coating layer to the predetermined stimulus includes one of exposing the polymer coating layer to a solution of a predetermined pH, exposing the polymer coating layer to a nucleophile, exposing the polymer coating layer to a solution containing a sugar, or exposing the polymer coating layer to a salt solution.

[0134] In yet another aspect, the method includes exposing a polymer coating layer on at least a portion of the flow cell support to a predetermined stimulus, thereby switching stimulus-responsive functional groups of the polymer coating layer: i) from a current state to a state that is more hydrophilic than the current state, or ii) from a neutral state to a charged state, or iii) from a folded state to an extended state, and performing a sequencing operation using the flow cell support when the polymer coating layer is in the more hydrophilic state, the charged state, or the extended state.

[0135] In some embodiments, the method is a method of making a flow cell, the method comprising applying a switchable heteropolymer to at least a portion of a flow cell support.

[0136] The addition of polymer (polymer coating layer) and primer (i.e., surface chemistry) to a patterned substrate is described with reference to Figures 1A to 1F, Figures 1G and 1H are described in combination with Figures 1A to 1D, and the addition of surface chemistry to an unpatterned substrate is described with reference to Figures 2A to 2D.

[0137] FIG. 1A is a cross-sectional view of one example of a patterned support 12. The patterned support 12 may be a patterned wafer, or a patterned die, or any other patterned support (e.g., a panel, a rectangular sheet, etc.). Any example of the support 12 described herein may be used. A patterned wafer may be used to form several flow cells, and a patterned die may be used to form a single flow cell. In one example, the support may have a diameter ranging from about 2 mm to about 300 mm, or may have a rectangular sheet or panel with a maximum dimension of up to about 10 feet (~3 meters). In one example, the support wafer has a diameter ranging from about 200 mm to about 300 mm. In another example, the support die has a width ranging from about 0.1 mm to about 10 mm. Although exemplary dimensions are provided, it should be understood that any suitable dimensions of support / substrate may be used. As another example, a panel may be used that is a rectangular support with a surface area larger than a 300 mm circular wafer.

[0138] Patterned substrate 12 includes depressions 14 defined on or in an exposed layer or surface of substrate 12 and interstitial regions 16 separating adjacent depressions 14. In the examples disclosed herein, depressions 14 are functionalized with surface chemistries (e.g., 20, 22), while interstitial regions 16 are available for binding but will not have a primer thereon (as shown in Figures 1E, 1F and 1H).

[0139] The depressions 14 may be fabricated in or on the substrate 12 using a variety of techniques including, for example, photolithography, nanoimprint lithography, stamping techniques, embossing techniques, molding techniques, microetching techniques, printing techniques, etc. As will be appreciated by those skilled in the art, the technique used will depend on the composition and shape of the substrate 12.

[0140] Many different layouts of the depressions 14 may be envisioned, including regular, repeating, and non-regular patterns. In one example, the depressions 14 are arranged in a hexagonal grid for close packing and improved density. Other layouts may include, for example, rectilinear (i.e., rectangular) layouts, triangular layouts, and the like. In some examples, the layout or pattern may be an xy format of the depressions 14, in rows and columns. In some other examples, the layout or pattern may be a repeating arrangement of the depressions 14 and / or interstitial regions 16. In yet other examples, the layout or pattern may be a random arrangement of the depressions 14 and / or interstitial regions 16. The patterns may include spots, pads, wells, posts, stripes, swirls, lines, triangles, rectangles, circles, arcs, checks, plaids, diagonals, arrows, squares, and / or cross hatches.

[0141] The layout or pattern may be characterized in terms of the density of the dimples 14 (i.e., the number of dimples 14) within a defined area. For example, the dimples 14 may be spaced apart from each other by 1 mm 2 The density may be, for example, at least about 1 mm 2 Approximately 100 per 1mm 2 Approximately 1,000 per 1mm 2 Approximately 100,000 per mm 2 Approximately 1 million per mm 2 Approximately 2 million per mm 2 Approximately 5 million per mm 2 Approximately 10 million per mm 2 Alternatively or additionally, the density may be adjusted to about 50 million or more per mm 2 Approximately 50 million per mm 2 Approximately 10 million per mm 2 Approximately 5 million per mm 2 Approximately 2 million per mm 2 Approximately 1 million per mm 2 Approximately 100,000 per mm 2 Approximately 1,000 per 1mm 2The density of the depressions 14 on the support 12 may be adjusted to about 100 per inch, or less. It should be further understood that the density of the depressions 14 on the support 12 may be between one of the low values ​​and one of the high values ​​selected from the ranges above. By way of example, a high density array may be characterized as having depressions 14 separated by less than about 100 nm, a medium density array may be characterized as having depressions 14 separated by about 400 nm to about 1 μm, and a low density array may be characterized as having depressions 14 separated by more than about 1 μm. Although examples of densities are provided, it should be understood that any suitable density substrate may be used.

[0142] The layout or pattern may also, or alternatively, be characterized in terms of the average pitch, i.e., the distance from the center of a recess 14 to the center of an adjacent interstitial region 16 (center-to-center spacing). The pattern may be regular such that the coefficient of variation of the average pitch is small, or the pattern may be irregular, in which case the coefficient of variation may be relatively large. In either case, the average pitch may be, for example, at least about 10 nm, about 0.1 μm, about 0.5 μm, about 1 μm, about 5 μm, about 10 μm, about 100 μm, or more. Alternatively or additionally, the average pitch may be, for example, up to about 100 μm, about 10 μm, about 5 μm, about 1 μm, about 0.5 μm, about 0.1 μm, or less. The average pitch of a particular pattern of sites 16 may be between one of the lower values ​​and one of the upper values ​​selected from the ranges above. In one example, the recesses 14 have a pitch (center-to-center spacing) of about 1.5 μm. Although examples of average pitch values ​​are provided, it should be understood that other average pitch values ​​may be used.

[0143] In the example shown in Figures 1A to 1H, the depressions 14 are wells 14', and thus the patterned support 12 includes an array of wells 14' on its surface. The wells 14' may be microwells or nanowells. The size of each well 14' may be characterized by its volume, well opening area, depth, and / or diameter.

[0144] Each well 14' can have any volume capable of holding liquid. The minimum or maximum volume can be selected to accommodate, for example, the expected throughput (e.g., multiplexing), resolution, analyte composition, or analyte reactivity for downstream use of the flow cell. For example, the volume can be at least about 1×10 -3 μm 3 , about 1×10 -2 μm 3 , about 0.1μm 3 , about 1μm 3 , about 10μm 3 , about 100μm 3 Alternatively or additionally, the capacity may be up to about 1×10 4 μm 3 , about 1×10 3 μm 3 , about 100μm 3 , about 10μm 3 , about 1μm 3 , about 0.1μm 3 , or less. It should be understood that the functionalized coating layer can fill all or a portion of the volume of the well 14'. The volume of the coating layer within an individual well 14' can be greater, less, or somewhere in between the values ​​specified above.

[0145] The area occupied by each well opening on the surface can be selected based on similar criteria as those discussed above for well volume. For example, the area of ​​each well opening on the surface can be at least about 1×10 -3 μm 2 , about 1×10 -2 μm 2 , about 0.1μm 2 , about 1μm 2 , about 10μm 2 , about 100μm 2 Alternatively or additionally, the area may be at most about 1×10 3 μm 2 , about 100μm 2 , about 10μm 2 , about 1μm 2, about 0.1μm 2 , about 1×10 -2 μm 2 The area occupied by each well opening may be greater than, less than, or between the values ​​specified above.

[0146] The depth of each well 14' may be at least about 0.1 μm, about 1 μm, about 10 μm, about 100 μm, or more. Alternatively or additionally, the depth may be at most about 1×10 3 The depth of each well 14' may be greater than, less than, or between the values ​​specified above.

[0147] In some cases, the diameter of each well 14' may be at least about 50 nm, about 0.1 μm, about 0.5 μm, about 1 μm, about 10 μm, about 100 μm, or more. Alternatively or additionally, the diameter may be at most about 1×10 3 μm, about 100 μm, about 10 μm, about 1 μm, about 0.5 μm, about 0.1 μm, or less (e.g., about 50 nm). The diameter of each well 14' may be larger, smaller, or between the values ​​specified above.

[0148] The patterned substrate 12 may be subjected to a series of processes to add surface chemistries 20, 22 to the recesses 14.

[0149] Although not shown, it should be understood that the patterned substrate 12 may be exposed to plasma ashing to clean and activate the surface. For example, the plasma ashing process may remove organic materials and introduce surface hydroxyl groups. Depending in part on the type of substrate 12, other suitable cleaning processes may be used to clean the substrate 12. For example, chemical cleaning may be performed using an oxidizing agent or a caustic solution.

[0150] The patterned support 12 (shown in FIG. 1A) may then be exposed to a process that prepares the surface for the placement of a stimuli-responsive polymer disclosed herein to form a polymer coating layer 20 (FIG. 1C). In one example, the patterned support 12 may be exposed to silanization, which bonds silanes or silane derivatives 18 (FIG. 1B) to the patterned support surface. The silanization introduces silanes or silane derivatives 18 to the entire surface, including the recesses 14, 14' (e.g., along the bottom and sidewalls) and the interstitial regions 16. In some embodiments, the silanes or silane derivatives are selectively introduced only to the recesses of a patterned substrate, or to microlocations of an unpatterned substrate, which are separated from one another.

[0151] Silanization can be accomplished using any silane or silane derivative 18. The choice of silane or silane derivative 18 may depend in part on the polymer used to form the polymer coating layer 20 (shown in FIG. 1C), since it may be desirable to form a covalent bond between the silane or silane derivative 18 and the polymer coating layer 20. The method used to bond the silane or silane derivative 18 to the substrate 12 may vary depending on the silane or silane derivative 18 used. Several examples are provided herein.

[0152] In one example, the silane or silane derivative 18 is (3-aminopropyl)triethoxysilane (APTES) or (3-aminopropyl)trimethoxysilane (APTMS) (i.e., XR B -Si(OR C )3, where X is amino and R B is -(CH2)3-, R Cis ethyl or methyl). In this example, the surface of the substrate 12 is pretreated with (3-aminopropyl)triethoxysilane (APTES) or (3-aminopropyl)trimethoxysilane (APTMS) to allow silicon to be covalently bonded to one or more oxygen atoms on the surface (without intending to be supported by the mechanism, each silicon may be bonded to one, two, or three oxygen atoms). This chemically treated surface is baked to form an amine group monolayer. The amine groups are then reacted with Sulfo-HSAB to form the azide derivative. 1 J / cm 2 to 30 J / cm 2 Upon UV activation at 21 °C with energy of 1000 keV, active nitrene species are generated, which can readily undergo a variety of intercalation reactions with the polymers disclosed herein.

[0153] Other silanization methods may also be used. Examples of suitable silanization methods include vapor deposition, YES, spin-coating, or other deposition methods. Although some examples of methods and materials that can be used to silanize the support 12 are described herein, it should be understood that other methods and materials can be used.

[0154] In an example utilizing a YES CVD oven, the patterned substrate 12 is placed in the CVD oven. The chamber may be evacuated and then the silanization cycle may be initiated. During the cycle, the silane or silane derivative container may be maintained at an appropriate temperature (e.g., about 120° C. for norbornene silane), the silane or silane derivative vapor line may be maintained at an appropriate temperature (e.g., about 125° C. for norbornene), and the vacuum line may be maintained at an appropriate temperature (e.g., about 145° C.).

[0155] In another example, silane or silane derivative 18 (e.g., liquid norbornene silane) may be deposited in a glass vial and placed in a glass vacuum desiccator along with the patterned substrate 12. The desiccator can then be evacuated to a pressure ranging from about 15 mTorr to about 30 mTorr and placed in an oven at a temperature ranging from about 60° C. to about 125° C. After silanization is allowed to proceed, the desiccator is removed from the oven, cooled, and vented to air.

[0156] Vapor deposition, the YES method and / or a vacuum desiccator may be used with a variety of silanes or silane derivatives 18, such as those silanes or silane derivatives 18 that contain the examples of unsaturated moieties disclosed herein. By way of example, these methods may be used where the silane or silane derivative 18 contains a cycloalkene unsaturated moiety, such as norbornene, norbornene derivatives (e.g., (hetero)norbornenes that contain oxygen or nitrogen in place of one of the carbon atoms), transcyclooctene, transcyclooctene derivatives, transcyclopentene, transcycloheptene, transcyclononene, bicyclo[3.3.1]non-1-ene, bicyclo[4.3.1]dec-1(9)-ene, bicyclo[4.2.1]non-1(8)-ene, and bicyclo[4.2.1]non-1-ene. Any of these cycloalkenes can be substituted with R groups such as, for example, hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, cycloalkynyl, aryl, heteroaryl, heteroalicyclyl, aralkyl, or (heteroalicyclyl)alkyl. An example of a norbornene derivative includes [(5-bicyclo[2.2.1]hept-2-enyl)ethyl]trimethoxysilane. As another example, these methods may be used when the silane or silane derivative 18 contains an alkyne or cycloalkyne unsaturated moiety, such as a cyclooctyne, a cyclooctyne derivative, or a bicyclononyne (e.g., bicyclo[6.1.0]non-4-yne or derivative, bicyclo[6.1.0]non-2-yne, or bicyclo[6.1.0]non-3-yne). These cycloalkynes can be substituted with any of the R groups described herein.

[0157] As shown in FIG. 1B, attachment of silane or silane derivative 18 forms a silanized patterned substrate that includes silanized recesses and silanized interstitial regions (which are one example of treated recesses and treated interstitial regions).

[0158] The silanized patterned substrate may then be exposed to a process that forms a polymer coating layer 20 over the silanized recesses and the silanized interstitial regions.

[0159] Prior to applying the polymer coating layer 20, some examples of the method may include synthesizing a heteropolymer that is disposed to form the polymer coating layer 20. The synthesis may include copolymerizing a stimuli-responsive functional group-containing monomer with a monomer selected from the group consisting of a diacetamidopentylacrylamide monomer, and a combination of an acrylamide monomer and an azidoacetamidopentylacrylamide monomer. Any of the pH-responsive, temperature-responsive, sugar-responsive, nucleophile-responsive, or salt-responsive monomers described herein may be used to form the heteropolymer. Several approaches may be used to make the polymeric materials disclosed herein. As some examples, the polymerization method used may be free radical polymerization, controlled radical polymerization, non-radical methods, or another suitable method.

[0160] As described herein, examples of the polymer coating layer 20 include any of the stimuli-responsive polymers disclosed herein, including any of the examples of stimuli-responsive functional groups and any of the examples of binding groups. The stimuli-responsive polymer may be present in a mixture or incorporated into a mixture. In one example, the mixture includes a stimuli-responsive polymer in a mixture of ethanol and water. The polymer coating layer 20 may be formed on the surface of the silanized patterned substrate 12 (i.e., on the silanized recesses and silanized interstitial regions) using any suitable technique. The stimuli-responsive polymer may be disposed on the surface of the patterned substrate 12 using chemical vapor deposition (CVD), or dipping or dip coating, dunk coating, spin coating, spray coating or ultrasonic spray coating, paddle dispensing, doctor blade coating, aerosol printing, screen printing, microcontact printing, or inkjet printing, or other suitable techniques. The polymer coating layer 20 is shown in FIG. 1C.

[0161] Dunk coating may involve submerging the patterned and silanized substrate in a series of temperature controlled baths. The baths may also be flow controlled and / or nitrogen blanketed. Through the various baths, the stimuli-responsive polymer bonds to form the polymer coating layer 20 in the silanized recesses and interstitial regions. In one example, the patterned and silanized substrate is introduced into a first bath containing a polymer mixture where a reaction to bond the polymers occurs, and then the patterned, silanized, polymer-coated substrate is transferred to an additional bath for washing. The patterned substrate may be moved from bath to bath using a robotic arm or manually. A drying system may be used for dunk coating.

[0162] Spray coating can be accomplished by spraying the polymer mixture directly onto the patterned and silanized substrate. The spray-coated substrate can be incubated for a time sufficient to allow the polymer to bind. After incubation, the unbound polymer mixture can be diluted and removed, for example, using a spin coater or by sonication in a bath or dunk tank as described herein.

[0163] Paddle dispensing can be performed according to the pool and spin-off method, and can therefore be accomplished using a spin coater. The polymer mixture can be applied (manually or via an automated process) to the patterned and silanized substrate. The applied polymer mixture can be applied or spread over the entire surface of the patterned and silanized substrate. The polymer-coated patterned substrate can be incubated for a time sufficient to allow the polymer to bind. After incubation, the unbound polymer mixture can be diluted and removed, for example, using a spin coater or by sonication in a bath or dunk tank as described herein.

[0164] The attachment of the polymer coating layer 20 to the silanized recesses and silanized interstitial regions (i.e., 18) may be via covalent bonds. The covalent attachment of the polymer coating layer 20 to the silanized recesses helps to maintain the polymer coating layer 20 in the recesses 14, 14' throughout the life of the ultimately formed flow cell during various uses. Below are some examples of reactions that may occur between silanes or silane derivatives 18 and the polymer coating layer 20:

[0165] When the silane or silane derivative 18 contains norbornene or a norbornene derivative as the unsaturated moiety, the norbornene or norbornene derivative can i) undergo 1,3-dipolar cycloaddition reaction with an azide / azide group of a stimuli-responsive polymer; ii) undergo a coupling reaction with a tetrazine group attached to a stimuli-responsive polymer; iii) undergo a cycloaddition reaction with a hydrazone group attached to a stimuli-responsive polymer; iv) undergo a photoclick reaction with a tetrazole group attached to a stimuli-responsive polymer; or v) undergo a cycloaddition reaction with a nitrile oxide group attached to a stimuli-responsive polymer.

[0166] When the silane or silane derivative 18 contains a cyclooctyne or cyclooctyne derivative as an unsaturated moiety, the cyclooctyne or cyclooctyne derivative can i) undergo strain-promoted azide-alkyne 1,3-cycloaddition (SPAAC) reaction with an azide / azide group of a stimuli-responsive polymer, or ii) undergo strain-promoted alkyne-nitrile oxide cycloaddition reaction with a nitrile oxide group attached to a stimuli-responsive polymer.

[0167] When the silane or silane derivative 18 contains a bicyclononyne as the unsaturated moiety, the bicyclononyne can undergo a similar SPAAC alkyne cycloaddition with azides or nitrile oxides attached to stimuli-responsive polymers due to the strain of the bicyclic ring system.

[0168] Although not shown, it should be understood that in some examples of the method, the patterned substrate 12 may not be exposed to silanization. Rather, the patterned substrate 12 may be exposed to plasma ashing, and then the polymer coating layer 20 may be spin-coated (or otherwise deposited) directly onto the plasma-ashed patterned substrate 12. In this example, the plasma ashing may generate surface-activating agents (e.g., -OH groups as hydroxyl or carboxyl) that can adhere the polymer coating layer 20 to the patterned substrate 12. In these examples, the other functional groups of the polymer coating layer 20 may be selected to react with the surface groups generated by the plasma ashing. For example, the other functional groups of the polymer coating layer 20 may be N-hydroxysuccinimide esters (NHS esters).

[0169] After being coated, the stimuli-responsive polymer may also be subjected to a curing process to form a polymer coating layer 20 over the patterned substrate (i.e., over the recesses 14 and interstitial regions 16). In one example, curing of the stimuli-responsive polymer may occur at a temperature ranging from room temperature (e.g., about 25° C.) to about 60° C., for a time ranging from about 5 minutes to about 2 hours.

[0170] The silanized and coated patterned substrate (as shown in FIG. 1C) may be subjected to a cleaning process. This process may utilize a water bath and ultrasonic treatment. The water bath may be maintained at a relatively low temperature ranging from about 22° C. to about 45° C. In another example, the temperature of the water bath ranges from about 25° C. to about 30° C.

[0171] The silanized and coated patterned support may then be subjected to polishing, if desired, to remove portions of the polymer coating layer 20 from the silanized interstitial regions. The silanized, coated and polished patterned substrate is shown in FIG. 1D. Portions of the silane or silane derivative 18 adjacent to the interstitial regions 16 may or may not be removed as a result of the polishing. Thus, in FIGS. 1D to 1H, portions of the silane or silane derivative 18 adjacent to the interstitial regions 16 are shown in phantom, as they may at least partially remain after polishing or may be removed after polishing. It should be understood that when these silanized portions are completely removed, the underlying support 12 is exposed.

[0172] The polishing process may be carried out with a mild chemical slurry (e.g., containing an abrasive, a buffer, a chelating agent, a surfactant, and / or a dispersant) capable of removing the thin polymer coating layer 20, and in some instances, at least some of the silane or silane derivative 18 from the interstitial regions 16, without adversely affecting the underlying substrate 12 in those regions. Alternatively, polishing may be performed with a solution that does not contain abrasive particles.

[0173] The chemical slurry may be used in a chemical mechanical polishing system to polish the surface of the silanized and coated patterned substrate shown in Figure 1C. The polishing head / pad or other polishing tool may polish the polymer coating layer 20 from the interstitial regions 16, leaving the polymer coating layer 20 in the recesses 14, 14' and leaving the underlying substrate 12 at least substantially intact. As one example, the polishing head may be a Strasborg ViPRRII polishing head.

[0174] As described above, polishing may be performed using a polishing pad and an abrasive-free solution. For example, a polishing pad may be utilized with a solution that does not contain abrasive particles (i.e., a solution that does not contain abrasive particles).

[0175] Polishing removes portions of the polymer coating layer 20 (and in some instances, at least a portion of the silane or silane derivative 18) from the interstitial regions 16, leaving portions of the polymer coating layer 20 in the silanized recesses, as shown in FIG. 1D. Also, as noted above, the interstitial regions 16 may remain silanized after polishing is completed. In other words, the silanized interstitial regions may remain intact after polishing. Alternatively (as shown by the phantom portion of 18), the silane or silane derivative 18 may be removed from the interstitial regions 16 as a result of polishing.

[0176] Although not shown, it should be understood that the silanized, coated, and polished patterned substrate (shown in FIG. 1D) may be subjected to a cleaning process. This process may utilize a water bath and sonication. The water bath may be maintained at a relatively low temperature, ranging from about 22° C. to about 30° C. The silanized, coated, and polished patterned substrate may be spin-dried or dried via another suitable technique.

[0177] The silanized, coated and polished patterned support shown in Figure 1D may then be subjected to a process to produce the flow cell 10 shown in Figures 1E and 1F, or may be subjected to a process to produce the flow cell 10' shown in Figures 1G and 1H. In Figures 1E and 1F, the primer 22 is grafted before the lid 26 is bonded to the patterned flow cell support 12. In Figures 1G and 1H, the lid 26 is bonded to the patterned flow cell support 12 before the primer 22 is grafted.

[0178] In FIG. 1E, a grafting process is performed to graft a primer 22 to the polymer coating layer 20 of the recesses 14, 14'. The primer 22 may be any forward or reverse amplification primer that includes an alkyne functional group. Specific examples of suitable primers include P5 and / or P7 primers, which are HISEQTM , HISEQX TM , MISEQ TM , MISEQDX TM ,MINISEQ TM , NEXTSEQ TM , NEXTSEQDX TM , NOVASEQ TM , GENOME ANALYZER TM , ISEQ TM and is used on the surface of commercially available flow cells sold by Illumina for sequencing on other instrument platforms.

[0179] In this example, grafting may be accomplished by flow-through deposition (e.g., using a temporarily attached lid), dunk coating, spray coating, paddle dispensing, or another suitable method of attaching the primer 22 to the functionalized polymer layer 20 in at least some of the recesses 14, 14'. Each of these exemplary techniques may utilize a primer solution or mixture that may include a primer, water, a buffer, and a catalyst, and may be performed as described herein.

[0180] Dunk coating may involve submerging the patterned substrate (with the polymer coating layer 20 in its recesses 14) in a series of temperature controlled baths. The baths may also be flow controlled and / or nitrogen blanketed. The baths may contain a primer solution or mixture. Throughout the various baths, the primer 22 bonds to the binding groups of the polymer coating layer 20 in at least some of the recesses 14. In one example, the coated and polished patterned substrate is introduced into a first bath containing a primer solution or mixture where a reaction occurs to bond the primer, and then the patterned substrate is transferred to an additional bath for cleaning. The patterned substrate may be moved from bath to bath using a robotic arm or manually. A drying system may be used for dunk coating.

[0181] Spray coating may be accomplished by spraying the primer solution or mixture directly onto the coated and polished patterned substrate. The spray coated wafer may be incubated at a temperature ranging from about 0° C. to about 70° C. for a time ranging from about 4 minutes to about 60 minutes. After incubation, the primer solution or mixture may be diluted and removed using, for example, a spin coater.

[0182] Paddle dispensing may be performed according to the pool and spin-off method and may therefore be accomplished using a spin coater. The primer solution or mixture may be applied (manually or via an automated process) to the coated and polished patterned substrate. The applied primer solution or mixture may be applied or spread over the entire surface of the coated and polished patterned substrate. The primer-coated patterned substrate may be incubated at a temperature ranging from about 0° C. to about 80° C. for a time ranging from about 2 minutes to about 60 minutes. After incubation, the primer solution or mixture may be diluted and removed, for example, using a spin coater.

[0183] 1F, the lid 26 may then be bonded to the bonding regions 25 of the support 12. If the patterned flow cell support 12 is a wafer, then different regions of the lid 26 may each at least partially define a flow channel 30 that is formed using the wafer. If the patterned flow cell support 12 is a die, then the lid 26 may define one or more flow channels 30 that are / are being formed.

[0184] The lid 26 may be any material that is transparent to the excitation light directed at the surface chemistries 20, 22 within the well 14. By way of example, the lid 26 may be glass (e.g., borosilicate, fused silica, etc.), plastic, etc. A commercially available example of a suitable borosilicate glass is D263 available from Schott North America, Inc.(R) A commercially available example of a suitable plastic material, i.e., a cycloolefin polymer, is ZEONOR 100, available from Zeon Chemicals L.P. (R) It is a product.

[0185] In some examples, the lid 26 may be integrally formed with a sidewall 29 that corresponds to the shape of the bonding region 25 and is bonded to the bonding region 25. For example, a recess may be etched into a transparent block to form a substantially planar (e.g., top) portion 27 and a sidewall 29 extending from the substantially planar portion 27. When the etched block is attached to the bonding region of the patterned substrate 12, the recess may become the flow channel 30.

[0186] In other examples, the sidewalls 29 and the lid 26 may be separate components bonded to one another. For example, the lid 26 may be a substantially rectangular block having at least a substantially planar outer surface and at least a substantially planar inner surface that defines a portion (e.g., a top) of the flow channel 30 (once bonded to the patterned support 12). The block may be bonded to a bonding region 25 of the patterned flow cell substrate 12 and attached (e.g., bonded) to the sidewalls 29 that form the sidewalls of the flow channel 30. In this example, the sidewalls 29 may include any of the materials described herein for the spacer layer (described below).

[0187] The lid 26 may be bonded to the bonding regions 25 of the patterned flow cell support 12 using any suitable technique, such as laser bonding, diffusion bonding, anodic bonding, eutectic bonding, plasma activated bonding, glass frit bonding, or methods known in the art. In one example, a spacer layer 28 may be used to bond the lid 26 to the bonding regions 25. The spacer layer 28 may be any material that seals at least some of the interstitial regions 16 (e.g., bonding regions 25) of the patterned substrate 12 and the lid 26 together.

[0188] In one example, the spacer layer 28 can be a radiation absorbing material that absorbs radiation at wavelengths transmitted by the lid 26 and / or the patterned support 12. The absorbed energy then forms bonds between the spacer layer 28 and the lid 26, and between the spacer layer 28 and the patterned substrate 12. An example of this radiation absorbing material is black KAPTON® (polyamide with carbon black) from DuPont (USA) that absorbs at about 1064 nm. It should be understood that polyimide can be used without the addition of carbon black, except that the wavelength must be changed to one that is significantly absorbed by the native polyimide material (e.g., 480 nm). As another example, polyimide CEN JP can be bonded when irradiated with 532 nm light. If the spacer layer 28 is a radiation absorbing material, the spacer layer 28 can be disposed at the interface between the lid 26 and the patterned support 12 such that the spacer layer 28 contacts the desired bond area 25. Compression (e.g., about 100 PSI pressure) may be applied while laser energy of an appropriate wavelength is applied to the interface (i.e., the radiation absorbing material is irradiated). Laser energy may be applied to the interface from above or below to achieve proper bonding.

[0189] In another example, the spacer layer 28 may include a radiation absorbing material in contact therewith. The radiation absorbing material may be applied to the interface between the spacer layer 28 and the lid 26, as well as the interface between the spacer layer 28 and the patterned flow cell support 12. As one example, the spacer layer 28 may be a polyimide and the separate radiation absorbing material may be carbon black. In this example, the separate radiation absorbing material absorbs laser energy that forms bonds between the spacer layer 28 and the lid 26, and between the spacer layer 28 and the patterned support 12. In this example, compression may be applied to each interface while laser energy of an appropriate wavelength is applied to the interfaces (i.e., while the radiation absorbing material is irradiated).

[0190] If the patterned flow support 12 is a wafer, the spacer layer 28 and sidewalls 29 (of or connected to the lid 26) may physically separate one flow channel 30 from an adjacent flow channel 30 and may be located on the periphery of the wafer. If the patterned support 12 is a die and the flow cell 10 being formed includes a single flow channel 30 or lane, the spacer layer 28 and sidewalls 29 (of or connected to the lid 26) may be located on the periphery of the die to define the flow channel 30 and seal the flow cell 10. If the patterned support 12 is a die and the flow cell 10 being formed includes multiple isolated flow channels 30 (e.g., eight or four flow channels / lanes), the spacer layer 28 and sidewalls 29 (of or connected to the lid 26) may physically separate one flow channel / lane 30 from an adjacent flow channel / lane 30 and may be located on the periphery of the die. However, it should be understood that the spacer layer 28 and sidewalls 29 may be located in any desired area depending on the implementation.

[0191] If the patterned support 12 is a die, assembly of the flow cell 10 may include bonding of the lid 26. If the patterned support 12 is a wafer, assembly of the flow cell 10 may include additional processing, such as dicing, after the lid 26 is bonded. In one example, the lid 26 may be bonded to the patterned wafer, and dicing forms the individual flow cells 10. As discussed herein, on the wafer, the sidewalls 29 may physically separate one flow channel 30 from an adjacent flow channel 30, and thus dicing may be performed through at least some of the sidewalls 29, such that each individual flow cell 10 includes a desired number of flow channels 30, with each flow channel 30 having a portion of the original sidewall 29 surrounding it. In another example, the patterned wafer may be diced to form a lidless die, which may have a respective lid 26 bonded thereto to form the individual flow cells 10.

[0192] In the exemplary flow cell 10 shown in Figure IF, the lid 26 includes a top 27 integrally formed with a sidewall 29. The sidewall 29 is bonded to a bonding region 25 of the patterned substrate 12 through a spacer layer 28.

[0193] Together, the lid 26 and the patterned flow cell substrate 12 define a flow channel 30 in selective fluid communication with the recesses 14, 14'. The flow channel 30 may function, for example, to selectively introduce reactive components or reactants to the surface chemistries 20, 22 to initiate designated reactions within / at the recesses 14, 14'.

[0194] Prior to performing a sequencing operation, the flow cell 10 may expose the polymer coating layer 20 to a predetermined stimulus to transition the polymer coating layer 20 from its current state to a more hydrophilic state (e.g., from a hydrophobic state to a hydrophilic state), from a neutral state to a charged state, and / or from a folded state to an extended state. The predetermined stimulus used will depend on the polymer coating layer 20 and the stimulus-responsive functional groups it contains. Exposing the polymer coating layer 20 to the predetermined stimulus may include heating the polymer coating layer 20; exposing the polymer coating layer 20 to a solution of a predetermined pH; exposing the polymer coating layer 20 to a solution containing a sugar, exposing the polymer coating layer 20 to a nucleophile; or exposing the polymer coating layer 20 to a salt solution. If the lid 26 is attached, exposing the polymer coating layer 20 to any solution may be accomplished by a flow-through process. For example, a basic or acidic solution, a sugar solution (e.g., glucose), or a salt solution may be introduced into the flow cell channel 30 via an input port, incubated for a time sufficient to effect a desired property change, and then removed from the channel 30 via an output port. In one example, the incubation time may be from a few seconds to a few minutes. If the stimuli-responsive functional group is thermoresponsive, the entire flow cell 10 may be heated, or a flow-through process may be used to expose the heated solution to the polymer coating layer 20.

[0195] The predetermined stimulus will render the polymer coating layer 20 more compatible with the conditions of the sequencing operation that is to be subsequently performed.

[0196] Examples of solutions of a given pH may include basic solutions such as 0.1 M NaOH, TRIS-HCL buffer, or carbonate buffer, or acidic solutions such as citrate buffer (pH 6) or 2-(N-morpholino)ethanesulfonic acid (MES) buffer. Examples of sugar solutions include glucose solutions having concentrations ranging from about 1 mM to about 100 mM. Examples of salt solutions include saline-sodium citrate buffer and phosphate buffered saline (PBS) buffer.

[0197] 1G and 1H, another example of a method involves bonding a lid 26 to the patterned flow cell support 12 before the primer 22 is grafted.

[0198] As shown in Figure 1G, a polymer coating layer 20 has been applied (e.g., deposited and polished) as described in Figure 1D. At least some of the polished interstitial regions 16 may define a bond region 25, and a lid 26 may be bonded to the bond region 25. The lid 26 may be any material and may have any configuration described herein. The lid 26 may be bonded to the bond region 25 via any of the techniques described herein.

[0199] In the example shown in Figure 1G, the lid 26 includes a top 27 integrally formed with sidewalls 29. The sidewalls 29 are bonded to bonding regions 25 of the patterned substrate 12 via spacers. After the lid 26 is bonded, a flow channel 30 is formed between the lid 26 and the patterned substrate 12. The flow channel 30 may function to selectively introduce various fluids into the flow cell 10' (Figure 1H).

[0200] Next, in this example, primers 22 are grafted to the polymer coating layer 20 in the recesses 14, as shown in FIG. 1H. Any of the primers 22 described herein may be used. In a flow-through process, a primer solution or mixture described herein may be introduced into the flow channel 30 via a respective input port (not shown), may be maintained within the flow channel 30 for a period sufficient for the primers 22 to attach to the polymer coating layer 20 in one or more recesses 14 (i.e., an incubation period), and may then be removed from a respective output port (not shown). After binding of the primers 22, additional fluid may be directed through the flow channel 30 to wash the now functionalized recesses and the flow channel 30.

[0201] Prior to performing a sequencing operation, the flow cell 10' may expose the polymer coating layer 20 to a predetermined stimulus to transition the polymer coating layer 20 from a current (e.g., more hydrophobic) state to a more hydrophilic state than the current state, from a neutral state to a charged state, and / or from a folded state to an extended state. The predetermined stimulus used will depend on the polymer coating layer 20 and the stimulus-responsive functional groups it contains. Exposing the polymer coating layer 20 to the predetermined stimulus may include heating the polymer coating layer 20, exposing the polymer coating layer 20 to a solution of a predetermined pH, exposing the polymer coating layer 20 to a solution containing a sugar, exposing the polymer coating layer 20 to a nucleophile, or exposing the polymer coating layer 20 to a salt solution. If the lid 26 is attached, exposing the polymer coating layer 20 to any solution may be accomplished by a flow-through process as previously described herein. If the stimuli-responsive functional group is thermally responsive, the entire flow cell 10' can be heated, or a flow-through process may be used in which a heated solution is exposed to the polymer coating layer 20.

[0202] The predetermined stimulus will render the polymer coating layer 20 more compatible with the conditions of the sequencing operation that is to be subsequently performed.

[0203] In other examples, exposing the polymer coating layer 20 to the predetermined stimulus may occur prior to grafting of the primer 22. In examples where the predetermined stimulus exposure occurs prior to grafting of the primer 22 in FIG. 1E, techniques other than a flow-through process, such as dip or dunk coating, may be used. For example, the silanized, coated, and polished patterned support shown in FIG. 1D may be immersed in a basic or acidic solution, a sugar solution (e.g., glucose), or a salt solution for a time sufficient for the desired property change. As another example, the silanized, coated, and polished patterned support shown in FIG. 1D may be heated to a desired temperature to initiate a state transition. In examples where the predetermined stimulus exposure occurs prior to grafting of the primer 22 in FIG. 1H, a flow-through process may be used for such exposure. As another example, the silanized, coated, and polished patterned support with the lid 26 attached may be heated to a desired temperature to initiate a state transition, as shown in FIG. 1G. Heating may be performed in the presence of water or a buffer solution.

[0204] As mentioned above, surface chemistry 20, 22 may also be added to the unpatterned support, and an example of this is described with reference to Figures 2A to 2D. In the case of the unpatterned support 12', the continuous surface would include the same surface chemistry 20, 22 as seen in the depressions 14' of Figures 1E, 1F and 1H. Any of the supports disclosed herein may be used as the unpatterned substrate 12', except that the unpatterned substrate 12' does not include depressions 14 or interstitial regions 16. In this exemplary method, a lid 26 (shown in Figure 2B) is first bonded to the unpatterned substrate 12' to form the flow channel 30. The lid 26 may be of any material and any configuration described herein. The lid 26 may also be bonded to the unpatterned substrate 12' via any of the techniques described herein.

[0205] 2B, the lid 26 includes a top 27 integrally formed with sidewalls 29. The sidewalls 29 are bonded to bonding regions 25 of the unpatterned substrate 12' via a spacer layer 28. The bonding regions 25 may be at the periphery of the unpatterned substrate 12' or in any area where it is desired to form a boundary for the flow channel 30. In other examples, the spacer layer 28 may form the sidewalls and may be attached to the at least substantially planar lid 26.

[0206] The lid 26 (including sidewalls 29) and the unpatterned substrate 12' together define a flow channel 30. The flow channel 30 may selectively introduce fluids to, for example, form the surface chemistries 20, 22, and may selectively introduce reactive components or reactants to the surface chemistries 20, 22 to initiate a state transition of the polymer coating layer 20 and / or to initiate other designated reactions within the flow channel 30.

[0207] Prior to forming the polymer coating layer 20 (shown in FIG. 2C), the method may include exposing the unpatterned substrate 12′ to a cleaning process (via a flow-through process) and / or another process (e.g., silanization) that prepares the exposed surface of the unpatterned substrate 12′ for subsequent deposition of a stimuli-responsive polymer.

[0208] Silanization of an unpatterned substrate 12' is shown in Figure 2B. In this example, silanization attaches silane or silane derivatives 18 to exposed portions of the unpatterned wafer surface 12' present in the flow channels 30.

[0209] Silanization may be accomplished using any silane or silane derivative 18. The choice of silane or silane derivative 18 may depend in part on the stimuli-responsive polymer used to form the polymer coating layer 20 (shown in FIG. 2C), since it may be desirable to form a covalent bond between the silane or silane derivative 18 and the polymer coating layer 20. The method used to attach the silane or silane derivative 18 to the substrate 12' may be a flow-through process.

[0210] As shown in FIG. 2C, in this example, within flow channel 30, a polymer coating layer 20 is then formed over the silane or silane derivative 18 or other chemical that was deposited to prepare the exposed surface of unpatterned substrate 12'.

[0211] Any of the stimuli-responsive polymers described herein may be used, and combinations of stimuli-responsive polymers may be used together. In one example, the formation of the polymer coating layer may be accomplished by a flow-through process. In a flow-through process, the stimuli-responsive polymers may be introduced into the flow channels 30 through their respective input ports and may or may not be cured. The polymer coating layer 20 is formed on the exposed surface of the unpatterned substrate 12', and no polishing is performed.

[0212] As shown in FIG. 2D, the primers 22 are grafted to the polymer coating layer 20 within the flow channel 30. In this example, the grafting may be accomplished by a flow-through process. In a flow-through process, a primer solution or mixture may be introduced into the flow channel 30 via the respective input ports and maintained within the flow channel for a sufficient time (i.e., an incubation period) for the primers 22 to bind to the binding groups of the polymer coating layer 20. The remaining primer solution or mixture may then be removed from the respective output ports. After primer binding, additional fluid may be directed through the flow channel to wash the now functionalized flow channel 30. The resulting flow cell 10'' in this example is shown in FIG. 2D.

[0213] Prior to performing a sequencing operation, the flow cell 10'' may be exposed to a predetermined stimulus of the polymer coating layer 20 to transition the polymer coating layer 20 from its current state to a more hydrophilic state (e.g., from a hydrophobic state to a hydrophilic state, from a hydrophilic state to a more hydrophilic state), from a neutral state to a charged state, and / or from a collapsed state to an expanded state. The predetermined stimulus used will depend on the polymer coating layer 20 and the stimulus-responsive functional groups it contains. Exposing the polymer coating layer 20 to the predetermined stimulus may include heating the polymer coating layer 20; exposing the polymer coating layer 20 to a solution of a predetermined pH; exposing the polymer coating layer 20 to a solution containing a sugar; exposing the polymer coating layer 20 to a nucleophile; or exposing the polymer coating layer 20 to a salt solution. Since the lid 26 is attached, exposing the polymer coating layer 20 to any solution can be accomplished by a flow-through process as previously described herein. If the stimuli-responsive functional group is thermally responsive, the entire flow cell 10'' can be heated, or a flow-through process may be used in which a heated solution is exposed to the polymer coating layer 20.

[0214] The predetermined stimulus will render the polymer coating layer 20 more compatible with the conditions of a subsequently performed sequencing operation. A sequencing operation is a process of determining the order of nucleotides in a sample of DNA or RNA. In one example, the sequencing operation is sequencing by synthesis, which involves imaging fluorescently labeled reversible terminators as nucleotides are added to a template strand, and then cleaving the fluorescently labeled reversible terminators to allow incorporation of the next base.

[0215] In another example using the flow cell 10'', exposing the polymer coating layer 20 to the predetermined stimulus may occur prior to grafting of the primer 22. Again, since the lid 26 is attached prior to applying the polymer coating layer 20, a flow-through process may be used for the exposure of the predetermined stimulus. As another example, as shown in FIG. 2C, the silanized and coated unpatterned substrate may be heated to a desired temperature to initiate a state transition. Heating may be accomplished in an aqueous environment (e.g., water or a buffer solution).

[0216] Although not shown, it should be understood that the patterned support 12 or the unpatterned support 12' may include inlet and outlet ports that fluidly engage with other ports (not shown) for directing fluids into and out of the respective flow channels (e.g., from a reagent cartridge or other fluid storage system) and from the flow channels (e.g., to a waste removal system).

[0217] Also, although not shown, it should be understood that the functionalized support (having surface chemistries 20, 22 thereon) may be bonded to another functionalized substrate having surface chemistries 20, 22 thereon instead of being bonded to the lid 26. The two functionalized surfaces may face each other and have a defined flow path between them. A spacer layer and appropriate bonding method may be used to bond the two functionalized substrates together.

[0218] The flow cells 10, 10', 10'' disclosed herein may be used with a variety of sequencing approaches or techniques, including techniques often referred to as sequencing by synthesis (SBS), cyclic array sequencing, sequencing by ligation, pyrosequencing, and the like. With any of these techniques, and in the example using a patterned support 12, the polymer coating layer 20 and attached primers 22 reside in the functionalized recesses (i.e., 14, 14' having surface chemistry 20, 22) and not in the interstitial regions 16, thereby confining amplification to the functionalized recesses. Sequencing generally involves hybridizing a nucleic acid template to the flow cell, amplifying the nucleic acid template, and detecting a signal when nucleotides or oligonucleotides bind to the amplified nucleic acid template.

[0219] As one example, sequencing-by-synthesis (SBS) reactions were performed using the HISEQ PCR kit from Illumina (San Diego, CA). (TM) , HISEQX (TM) , MISEQ (TM) , MISEQDX (TM) ,MINISEQ (TM) , NOVASEQ (TM) , NEXTSEQX (TM) , or NEXTSEQ (TM) It may be performed in a system such as a sequencer system.

[0220] The SBS sequencing operation generally includes introducing a nucleic acid library template to the flow cell support 12, whereby the nucleic acid library template hybridizes to a primer 22 attached to a polymer coating layer 20; generating a nucleic acid template strand from the hybridized nucleic acid library template; introducing a sequencing primer complementary to an adapter of the nucleic acid template strand; introducing fluorescently labeled nucleotides and a polymerase to the flow cell support 12, whereby one of the fluorescently labeled nucleotides is incorporated to extend the sequencing primer along the nucleic acid template strand; and detecting a fluorescent signal from one of the incorporated fluorescently labeled nucleotides.

[0221] In SBS, multiple nucleic acid library templates may be introduced to the flow cell 10, 10', 10''. The multiple nucleic acid library templates, for example, hybridize to one of two types of primers 22 immobilized on the flow cell 10, 10', 10''. In one example of cluster generation, the nucleic acid library templates are copied by 3' extension from the hybridized primer 22 using a high fidelity DNA polymerase. The original nucleic acid library template is denatured and the copy remains immobilized where the primer 22 is located. Isothermal bridge amplification may be used to amplify the immobilized copy. For example, the copied template loops over and hybridizes to an adjacent complementary primer 22, and the polymerase copies the copied template to form a double-stranded bridge, which is denatured to form two single strands. These two strands loop over and hybridize to adjacent complementary primers 22 and are extended again to form two new double-stranded loops. This process is repeated for each template copy by cycles of isothermal denaturation and amplification to create dense clonal clusters. In one example, the reverse strand is removed by specific base cleavage, leaving the forward template strand.

[0222] The 3' end of the template and optional primer 22 can be blocked to prevent undesired priming. A sequencing primer can be introduced into the flow cell 10, 10', 10''. The sequencing primer is complementary to the adapter of the nucleic acid template strand and therefore hybridizes to the adapter (e.g., the lead 1 sequencing primer of the template).

[0223] The extension of a nucleic acid primer (e.g., a sequencing primer) along a nucleic acid template (e.g., a forward template polynucleotide strand) is monitored to determine the sequence of nucleotides in the template. The underlying chemical process can be polymerization (e.g., catalyzed by a polymerase enzyme) or ligation (e.g., catalyzed by a ligase enzyme). In certain polymerase-based SBS processes, fluorescently labeled nucleotides are added to the template in a template-dependent manner (thereby extending the sequencing primer), and detection of the order and type of nucleotides added to the sequencing primer can be used to determine the sequence of the template. For example, to initiate a first SBS cycle, one or more labeled nucleotides, a DNA polymerase, or the like, can be delivered into / through a flow channel 30, such as a flow channel 30 that houses an array of primers 22 having template strands bound thereto. Sequencing primer extension causes the incorporation of labeled nucleotides, and this incorporation can be detected through an imaging event. During an imaging event, an illumination system (not shown) can provide excitation light to the flow cell 10, 10', 10''.

[0224] In some instances, the nucleotides may further include a reversible termination feature that terminates further sequencing primer extension once the nucleotide is added. For example, a nucleotide analog with a reversible terminator portion may be added to the sequencing primer along the template strand, such that no further extension can occur until a deblocking agent is delivered to remove the portion. Thus, for instances using a reversible termination, a deblocking agent may be delivered to the flow channel 30 or the like (before or after detection is performed).

[0225] Washing may be performed between the various fluid delivery steps. The SBS cycle is then repeated n times to extend the sequencing primer by n nucleotides, thereby allowing detection of a sequence of length n.

[0226] Although SBS is described in detail, it should be understood that the flow cells 10, 10', 10'' described herein may be utilized with other sequencing protocols, such as flow cell-based library preparation for genotyping or in other chemical and / or biological applications.

[0227] To further illustrate the present disclosure, examples and prophetic examples are given herein. It should be understood that these examples are provided for illustrative purposes and should not be construed as limiting the scope of the present disclosure. Non-Limiting Illustrative and Prophetic Examples Example 1. Synthesis of sugar-responsive switchable heteropolymers (boronic acids). [ka]

[0228] A mixture of BrAPA (N-(5-bromoacetamidylpentyl)acrylamide), sodium azide, and dimethylformamide (DMF) was placed in a DrySyn bath and the solution was heated under nitrogen atmosphere at 20 °C with stirring for 3 h to produce AzAPA (N-(5-azidoacetamidylpentyl)acrylamide). Acrylamide and 3-(acrylamido)phenylboronic acid were dissolved in deionized water. The prepared AzAPA solution was then added to the acrylamide / 3-(acrylamido)phenylboronic acid solution and mixed thoroughly before filtering through a 0.2 μm filter. The filtered solution was then transferred to a 500 mL round-bottom flask equipped with a stir bar and nitrogen was bubbled through the mixture for 30 min. While the acrylamide / AzAPA premix was degassed, the required amount of potassium persulfate in deionized water was prepared and transferred to the mixture of monomers. The mixture was then treated with the co-initiator TEMED (tetramethylethylenediamine). The solution was stirred under nitrogen at 35° C. for 1.5 hours. At the end of the polymerization, the nitrogen gas line was removed and the reaction flask was exposed to air. The crude mixture was then slowly added to 2-propanol. The crude polymer was then isolated by filtration.

Claims

1. an acrylamide monomer that includes a linking group that reacts with a functional group attached to the primer; and A heteropolymer comprising a monomer comprising a stimulus-responsive functional group, the linking group of the acrylamide monomer comprises an azide group, and the acrylamide monomer is an azidoacetamidepentylacrylamide monomer; Here, the monomer containing a stimuli-responsive functional group is a temperature-responsive N-substituted acrylamide, wherein the temperature-responsive N-substituted acrylamide is N-isopropylacrylamide; an acrylamide, acrylate, or vinyl monomer comprising a terminal sugar-responsive functional group, wherein the terminal sugar-responsive functional group comprises a boronic acid group; and an acrylamide, acrylate, or vinyl monomer comprising a terminal nucleophile-responsive functional group, said terminal nucleophile-responsive functional group having the structure: 【Chemistry 1】 Here, (a) Y is SO ２ and Y' is CH ２ or (b) Y and Y' are both C(O).

2. The heteropolymer of claim 1, wherein the monomer containing the stimuli-responsive functional group undergoes modification upon exposure to a predetermined stimulus, wherein the modification changes the polarity and / or conformation of the heteropolymer.

3. The heteropolymer of claim 1 or 2, further comprising a primer grafted to said linking group.

4. 4. The heteropolymer of claim 1, wherein the linking group is selected from the group consisting of an azide group, an alkenyl group, an alkynyl group, an aldehyde group, a hydrazone group, a hydrazine group, a tetrazole group, a tetrazine group, and a thiol group.

5. 10. The heteropolymer of claim 1 further comprising a second acrylamide monomer.

6. 2. The heteropolymer according to claim 1, wherein the monomer containing a stimuli-responsive functional group is 3-(acrylamide)phenylboronic acid.

7. a temperature-responsive N-substituted acrylamide, wherein the temperature-responsive N-substituted acrylamide is N-isopropylacrylamide; an acrylamide, acrylate, or vinyl monomer comprising a terminal sugar-responsive functional group, wherein the terminal sugar-responsive functional group comprises a boronic acid group; and an acrylamide, acrylate, or vinyl monomer comprising a terminal nucleophile-responsive functional group, said terminal nucleophile-responsive functional group having the structure: 【Chemistry 2】 Here, (a) Y is SO ２ and Y' is CH ２ or (b) Y and Y′ are both C(O); copolymerizing the monomer containing the stimulus-responsive functional group with an acrylamide monomer containing a linking group that reacts with a functional group attached to the primer; The method for preparing a switchable heteropolymer, wherein the acrylamide monomer containing a linking group is an azidoacetamidepentylacrylamide monomer.

8. 8. The method of claim 7, further comprising copolymerizing the monomer containing the stimulus-responsive functional group and the acrylamide monomer containing the linking group with a second acrylamide monomer.

9. A support, and A flow cell comprising a heteropolymer according to any one of claims 1 to 6 attached to said support.

10. 10. A method of making a flow cell comprising contacting a heteropolymer of any one of claims 1, 2 or 4-6 with at least a portion of a flow cell support, thereby binding said heteropolymer to the flow cell support.

11. The method of claim 10, further comprising grafting a primer onto the attachment group of the support-bound heteropolymer.

12. 12. The method of claim 10 or 11, further comprising exposing the heteropolymer bound to the flow cell support to a predetermined stimulus.

13. 13. The method of claim 12, further comprising performing a sequencing operation on the flow cell after exposing.

14. 10. Grafting a primer onto a switchable heteropolymer according to any one of claims 1 to 6 on a flow cell support; exposing the switchable heteropolymer on the flow cell support to a predetermined stimulus, thereby causing a change in the polarity and / or conformation of the switchable heteropolymer; hybridizing a nucleic acid template to a primer on a flow cell support; amplifying the nucleic acid template on the flow cell support to produce an amplified template; and Detecting a signal when the labeled nucleotide binds to a complementary nucleotide of the amplified template. A sequencing method comprising:

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