Collagen enhancer

Abstract

To provide a new material that exhibits the effect of increasing collagen even when applied to organisms.SOLUTION: Oren-gedoku-to extract can increase the amount of collagen even when applied to organisms, so that it can be used as an active ingredient of an agent for increasing collagen.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a collagen enhancer.

Background Art

[0002] Collagen is a type of extracellular matrix and is essential not only for maintaining the form of tissues and organs but also plays an important role under physiological conditions such as wound healing and tissue repair processes. Among them, collagen in the skin decreases due to aging and light stimulation, etc., and is one of the causes of wrinkles and sagging, which are manifestations of skin aging.

[0003] Typical examples of components formulated to promote the production of collagen, which functions to give elasticity to the skin, are retinoic acid and retinol. Retinoic acid is used as an active ingredient in anti-wrinkle pharmaceuticals, but since it is highly stimulating, a doctor's prescription is required. The strength of the physiological activity of retinol is about 1 / 100 that of retinoic acid, and because of its high safety, it is formulated in cosmetics and used.

[0004] In recent years, research has been conducted on other materials having the effect of increasing collagen. For example, Patent Document 1 discloses a composition for promoting collagen production containing cells of Pediococcus acidilactici or a processed product thereof, and Patent Document 2 discloses a collagen production promoter characterized by containing an aqueous extract of Lilium rubellum as an active ingredient.

Prior Art Documents

Patent Documents

[0005]

Patent Document 1

Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0006] As described above, retinoic acid has a problem of irritation, and retinol has a problem that its effect is not so high. In addition, the materials described in Patent Documents 1 and 2 have only been confirmed to be effective at the cell level, and no indication is given as to how effective they are when actually applied to a living body.

[0007] Therefore, an object of the present invention is to provide a new material that shows an effect of increasing collagen even when applied to a living body.

Means for Solving the Problems

[0008] As a result of intensive studies, the present inventor has found that the extract of Huanglian Jiedu Decoction shows an effect of increasing collagen even when applied to a living body. The present invention has been completed by further studies based on such findings.

[0009] That is, the present invention provides an invention in the following aspects. Item 1. A collagen increasing agent containing an extract of Huanglian Jiedu Decoction. Item 2. The collagen increasing agent according to Item 1, which is used to increase the amount of collagen in the dermis of the skin. Item 3. The collagen increasing agent according to Item 1 or 2, which is used to increase the amount of collagen in the outer corner of the eye.

Effects of the Invention

[0010] According to the present invention, a new material that shows an effect of increasing collagen even when applied to a living body is provided.

Brief Description of the Drawings

[0011]

Figure 1

Figure 2

BEST MODE FOR CARRYING OUT THE INVENTION

[0012] The collagen enhancer of the present invention is characterized by containing an extract of Huanglian Jiedu Decoction. Hereinafter, the collagen enhancer of the present invention will be described in detail.

[0013] Coptis chinensis decoction extract The collagen enhancer of the present invention contains an extract of Huanglian Jiedu Decoction as an active ingredient. Huanglian Jiedu Decoction is a mixed crude drug consisting of Scutellaria baicalensis Georgi, Gardenia jasminoides Ellis, Coptis chinensis Franch., and Phellodendron amurense Rupr., with the "Essential Formulas from the Golden Cabinet" as the original text.

[0014] In the present invention, the mixing ratio of the crude drugs constituting Huanglian Jiedu Decoction is not particularly limited, but usually, Scutellaria baicalensis Georgi is 1.5 to 4 parts by weight, preferably 2 to 3 parts by weight; Gardenia jasminoides Ellis is 0.5 to 3 parts by weight, preferably 0.75 to 2 parts by weight; Coptis chinensis Franch. is 0.5 to 4 parts by weight, preferably 1 to 3 parts by weight; Phellodendron amurense Rupr. is 0.5 to 4 parts by weight, preferably 0.75 to 3 parts by weight.

[0015] Suitable examples of the crude drug preparation used for the production of the extract of Huanglian Jiedu Decoction in the present invention include 3 parts by weight of Scutellaria baicalensis Georgi, 2 parts by weight of Coptis chinensis Franch., 1.5 parts by weight of Gardenia jasminoides Ellis, and 1.5 parts by weight of Phellodendron amurense Rupr.

[0016] The form of the extract of Huanglian Jiedu Decoction may be any of liquid extracts such as fluid extract and soft extract, or solid dried extract powder.

[0017] The liquid extract of Huanglian Jiedu Decoction can be obtained by subjecting the mixed crude drug according to Huanglian Jiedu Decoction to an extraction treatment and concentrating the obtained extract as necessary. The extraction solvent used for the extraction treatment is not particularly limited, and examples include water or aqueous ethanol, preferably water. Further, the dried extract powder of Huanglian Jiedu Decoction can be obtained by drying the liquid extract. The method of the drying treatment is not particularly limited, and examples include spray drying method, and a method of adding a suitable adsorbent (such as anhydrous silicic acid, starch, etc.) to a soft extract with a high extract concentration to obtain an adsorbed powder.

[0018] In the present invention, as the Coptidis Rhizoma and Gardeniae Fruit Decoction extract, the extract prepared by the aforementioned method may be used, or a commercially available product may be used. For example, as the dried extract powder of Coptidis Rhizoma and Gardeniae Fruit Decoction, Coptidis Rhizoma and Gardeniae Fruit Decoction Dried Extract - AT (manufactured by Nippon Powdered Drugs Co., Ltd.), Coptidis Rhizoma and Gardeniae Fruit Decoction Dried Extract - F (manufactured by Alps Pharmaceutical Co., Ltd.), etc. are known as products and can also be obtained commercially.

[0019] In the collagen enhancer of the present invention, the content of the Coptidis Rhizoma and Gardeniae Fruit Decoction extract is not particularly limited as long as the effects of the present invention are achieved. However, in terms of the amount of the dried extract powder of the Coptidis Rhizoma and Gardeniae Fruit Decoction, it is usually 10 to 100% by weight, preferably 20 to 90% by weight, more preferably 40 to 80% by weight, and still more preferably 60 to 70% by weight. In the present invention, the conversion in terms of the amount of the dried extract powder of the Coptidis Rhizoma and Gardeniae Fruit Decoction means that when the dried extract powder of the Coptidis Rhizoma and Gardeniae Fruit Decoction is used, it is the amount itself, and when the liquid extract of the Coptidis Rhizoma and Gardeniae Fruit Decoction is used, it is the amount converted to the remaining amount after removing the solvent. Further, when the dried extract powder of the Coptidis Rhizoma and Gardeniae Fruit Decoction contains additives such as adsorbents added during production, it is the amount excluding the additives.

[0020] Other components The collagen enhancer of the present invention may consist solely of the extract of Huanglian Jiedu Decoction, or may contain additives and bases according to the dosage form. Such additives and bases are not particularly limited as long as they are pharmaceutically acceptable. For example, excipients, binders, disintegrants, lubricants, isotonic agents, plasticizers, dispersants, emulsifiers, solubilizers, wetting agents, stabilizers, suspending agents, adhesives, coating agents, gloss agents, water, fats and oils, waxes, hydrocarbons, fatty acids, higher alcohols, esters, water-soluble polymers, surfactants, metal soaps, lower alcohols, polyhydric alcohols, pH adjusters, buffers, antioxidants, ultraviolet ray inhibitors, preservatives, flavoring agents, fragrances, powders, thickeners, pigments, chelating agents, etc. may be mentioned. These additives may be used alone or in combination of two or more. Also, the contents of these additives and bases are appropriately set according to the types of additives and bases used, the dosage form of the collagen enhancer, etc.

[0021] In addition to the extract of Huanglian Jiedu Decoction, the collagen enhancer of the present invention may, if necessary, contain other nutritional components and pharmacological components. Such nutritional components and pharmacological components are not particularly limited as long as they are pharmaceutically acceptable. For example, antacids, stomachics, digestive agents, intestinal regulators, antispasmodics, mucosal repair agents, anti-inflammatory agents, astringents, antiemetics, antitussives, expectorants, anti-inflammatory enzyme agents, sedative hypnotics, antihistamines, caffeine compounds, cardiotonic diuretics, antibacterial agents, vasoconstrictors, vasodilators, local anesthetics, crude drug extracts, vitamins, menthol compounds, etc. may be mentioned. These nutritional components and pharmacological components may be used alone or in combination of two or more. Also, the contents of these components are appropriately set according to the types of components used, etc.

[0022] Dosage form Regarding the dosage form of the collagen enhancer of the present invention, there is no particular limitation as long as oral administration is possible. For example, solid preparations such as powders, fine granules, granules, tablets, troches, chewable tablets, capsules (soft capsules, hard capsules), pills, etc.; semi-solid preparations such as jelly; liquid preparations such as solutions, suspensions, syrups, etc. are mentioned. Preferably, granules or tablets are mentioned.

[0023] Manufacturing method The method for producing the collagen enhancer of the present invention may be formulated according to the usual formulation methods employed in the pharmaceutical field using the above-mentioned crude drug components.

[0024] Use The collagen enhancer of the present invention is used for the purpose of increasing the amount of collagen.

[0025] The tissue or organ for which the amount of collagen is to be increased is not particularly limited, and examples include the dermal layer of the skin, gums, cartilage, etc. Preferably, the dermal layer of the skin is mentioned. Further, when the collagen enhancer of the present invention is used to increase the amount of collagen in the dermal layer of the skin, the location of the dermal layer of the skin on the body surface is not particularly limited, and it may be any of the face, neck, trunk, upper limbs, and lower limbs. Preferably, the face is mentioned, and more preferably, the outer canthus is mentioned.

[0026] Dosage and administration The collagen enhancer of the present invention is used by oral administration. Regarding the dosage of the collagen enhancer of the present invention, it is appropriately set according to the age, constitution, degree of symptoms, etc. of the administration subject. For example, for one human, the amount of Coptis chinensis Detoxification Decoction extract (in terms of dry extract) is 500 to 2000 mg, preferably 800 to 1800 mg per day, and it may be taken 1 to 4 times a day, preferably 2 to 3 times a day.

[0027] Regarding the timing of taking the agent within a day, there is no particular limitation, and it may be taken before meals, after meals, or between meals, but preferably before meals or between meals. Further, from the viewpoint of further enhancing the collagen increasing effect, the collagen increasing agent of the present invention is preferably taken continuously for, for example, 3 days or more, preferably 5 days or more, and more preferably 10 days or more.

Examples

[0028] Hereinafter, the present invention will be specifically described by way of examples, but the present invention is not limited to these examples.

[0029] [Test Example 1] (1) Preparation of collagen increasing agent As raw crude drugs, 3 parts by weight of Scutellaria baicalensis Georgi, 2 parts by weight of Cnidium officinale Makino, 1.5 parts by weight of Rheum palmatum L., and 1.5 parts by weight of Paeonia lactiflora Pall. were used. After grinding these, 10 times the weight of water was used for extraction at about 100 ° C for 1 hour, and centrifugation was performed to obtain an extract. The extract was concentrated under reduced pressure and dried using a spray dryer to obtain an extract powder of Huanglian Jiedu Decoction. The drying by the spray dryer was performed by dropping the extract onto an atomizer with a rotation speed of 10,000 rpm and supplying hot air of air at 150 ° C. Thereafter, granulation was performed and tablets were prepared to obtain a collagen increasing agent. The daily dose of the collagen increasing agent was 1600 mg in terms of the amount of Huanglian Jiedu Decoction extract (dry extract equivalent).

[0030] (2) Experimental method Nine men and women in their 20s were made to take the above-mentioned collagen increasing agent for 10 days (twice a day). The collagen score of the left outer canthus was measured before and after taking using an ultrasonic dermography device (device name: DermaLab (registered trademark), model number: DermaLab, manufactured by Cortex Technology, measurement item: collagen score). The measurement was performed 3 times each, and the average value was taken as the measured value (the measured values of the collagen score were made to be within ±5 for two times. The room temperature was 20 ° C and the humidity was 65%, and the measurement was performed after 15 minutes of acclimation).

[0031] An example of the obtained ultrasonic dermal images (before and after administration) is shown in Fig. 1. In each of the images shown in Fig. 1, the left side indicates the stratum corneum side and the left side indicates the dermal layer side. In this ultrasonic dermal image, the areas with high density of dermal collagen fibers are shown in yellow and the areas with low density are shown in green. As shown in Fig. 1, it was confirmed that the amount of collagen increased significantly after administration.

[0032] Also, Fig. 2 shows the results of calculating the mean value ± standard error of the measurement results of the collagen score and performing a paired t-test using Microsoft (registered trademark) Excel (statistical analysis software: Excel Statistics). As shown in Fig. 2, a significant (*: p < 0.05) increase in the collagen score was confirmed. From this result as well, it was confirmed that the amount of collagen increased after administration.

[0033] (3) Comparison with the ability to increase collagen by a mixture of Coptidis Rhizoma extract and Phellodendri Cortex extract The collagen increasing agent of the present invention is excellent in the collagen increasing effect, and the Coptidis Rhizoma Decoction extract acts as an active ingredient. This can be confirmed by the fact that when a mixture of extracts of Coptis chinensis and Phellodendron amurense, which are the constituent crude drugs of Coptidis Rhizoma Decoction (a mixture consisting of Coptidis Rhizoma extract and Phellodendri Cortex extract), and the Coptidis Rhizoma Decoction extract are each subjected to the following cell test, the Coptidis Rhizoma Decoction extract exhibits a superior collagen increasing effect.

[0034] (Cell test method) Normal human dermal fibroblasts (NHDF) are cultured in a 24-well culture plate. More specifically, 1.0×10 4Seed the plate at the density of cells / wells and culture at 37 °C for 72 hours in an environment of 5% carbon dioxide and 95% air. As the culture medium, use a medium containing fetal bovine serum (FBS) at a concentration of 2% by mass in Dulbecco's Modified Eagle Medium (DMEM), and add the concentration of Coptidis Rhizoma Decoction extract and the concentration of a mixture consisting of Evodiae Fructus extract and Paeoniae Radix Alba extract (mixed weight ratio of Evodiae Fructus extract and Paeoniae Radix Alba extract = 1:1) (that is, the total amount of Evodiae Fructus extract and Paeoniae Radix Alba extract) to be 0.01% by mass, respectively. After the culture is completed, measure the amount of collagen in the culture supernatant by ELISA.

Claims

Claim 1 An oral collagen increasing agent containing an aqueous extract of Huanglian Jiedu Decoction. Claim 2 The collagen increasing agent according to claim 1, which is used to increase the amount of collagen in the dermis of the skin. Claim 3 The collagen increasing agent according to claim 1 or 2, which is used to increase the amount of collagen in the outer canthus.

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