Method for producing exosomes

The method of using a polyimide porous membrane in a suspended culture system with oscillating movements addresses the challenge of maintaining exosome quality and yield in industrial-scale production, ensuring stable and efficient exosome production.

JP7711386B2Active Publication Date: 2025-07-23UBE CORPORATION
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Patent Information

Application Number
JP2021018631
Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
Filing Date
2021-02-08
Publication Date
2025-07-23
Estimated Expiration
2041-02-08

AI Technical Summary

Technical Problem

Existing methods for producing exosomes lack the ability to maintain uniform quality and high yield over an extended period, especially in industrial-scale production, due to changes in cell culture conditions and the aging of primary cells, which complicates the establishment of a stable exosome production system.

Method used

A method involving a polyimide porous membrane housed in a casing, suspended in a medium without being fixed, and subjected to oscillating and stirring movements to culture cells, ensuring stable exosome production over time.

Benefits of technology

This approach allows for the continuous production of exosomes with uniform quality and high yield, suitable for industrial-scale use, while maintaining cell characteristics and preventing cell aging.

✦ Generated by Eureka AI based on patent content.

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Abstract

To provide: a method for producing at high yield, exosome having uniform quality, for a long term and continuously, using cells; an exosome production device; a kit; use thereof; and exosome produced using them.SOLUTION: There is provided a method for producing exosome using cells, the method comprises: a step of applying the cells to a cell culture module; and a step of floating the cell culture module to which cells are applied, in a medium in a state in which, the module is not fixed into a cell culture vessel for floating culture, for culturing cells by shaking and / or stirring the cell culture module by turning motion, reciprocation motion, vertical motion, wave motion typed oscillation, rotary motion or combination thereof, for producing the exosome by the cells. The cell culture module comprises: a polyimide porous film; and a casing comprising two or more medium outflow inlets and storing the polyimide porous film.SELECTED DRAWING: None
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Citation Information

Patent Citations

  • Method for detecting cancer, and cancer inhibitor

    JP2009171876A

  • Porous polyimide film and method for producing the same

    JP2011219585A

  • Porous polyimide film and method for producing the same

    JP2011219586A

  • Method of creating exosome from stem cells and use thereof

    JP2018064550A

  • Exosomes and micro-ribonucleic acids for tissue regeneration

    JP2019038840A