Compositions containing plant extracts

Abstract

The present invention relates to a composition containing a plant extract, which has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in skin melanocytes, promotes collagen synthesis in skin fibroblasts and inhibits collagenase activity, thereby promoting skin regeneration and improving skin elasticity and wrinkles, inhibits NO production, thereby exhibiting anti-inflammatory and skin soothing effects, promotes hyaluronic acid production in fibroblasts, thereby increasing skin moisture content and providing moisturizing effects, scavenges free radicals, thereby exhibiting antioxidant effects, and exhibits a wide range of antibacterial effects against various bacteria, making it suitable for use as a cosmetic composition, pharmaceutical composition, topical skin preparation, or food composition.

Description

[Technical Field]

[0001] The present invention relates to a composition containing a plant extract, which has skin whitening, skin regeneration, improvement of skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant or antibacterial effects. [Background technology]

[0002] Everyone desires fair, beautiful skin. This is genetically determined by the concentration and distribution of melanin in the skin, but it is also influenced by environmental and physiological conditions such as ultraviolet rays from the sun, fatigue, and stress. Melanin is produced when the amino acid tyrosine is converted by the enzyme tyrosinase into dopa and dopaquinone, which are then converted through a non-enzymatic oxidation reaction. While the pathway by which melanin is produced is known, the mechanism that induces melanin synthesis, the step before tyrosinase action, remains unclear.

[0003] Commonly known skin-whitening ingredients include substances that inhibit the activity of tyrosinase enzymes, such as kojic acid and arbutin, hydroquinone, vitamin C (L-ascorbic acid), and their derivatives, as well as various plant extracts. These not only brighten skin tone by inhibiting the synthesis of melanin pigment, but also improve hyperpigmentation, such as age spots and freckles caused by ultraviolet rays, hormones, or genetics. However, there are issues with safety issues, such as irritation and redness when applied to the skin, which limit the amount that can be used, or the effect is so minimal that substantial results cannot be expected.

[0004] Collagen is a major matrix protein produced by skin fibroblasts and is present in the extracellular matrix. Its important functions include providing mechanical strength to the skin, resistance and cohesion of connective tissue, supporting cell adhesion, and inducing cell division and differentiation (during organismal growth or wound healing). Collagen decreases with age and photoaging due to UV exposure, which is known to be closely related to the formation of wrinkles in the skin. Furthermore, recent extensive research on skin aging has revealed the important functions of collagen in the skin.

[0005] Active ingredients that promote collagen synthesis and improve wrinkles are known. For example, retinoic acid, transforming growth factor (TGF) [Non-Patent Document 1], animal placenta-derived proteins [Patent Document 1], betulinic acid [Patent Document 2], and chlorella extract [Patent Documents 3 and 4] are known to promote collagen synthesis. However, these active ingredients have safety issues, such as irritation and redness when applied to the skin, and are therefore limited in their dosage. Furthermore, their efficacy is so minimal that they cannot be expected to substantially promote collagen synthesis in the skin and improve skin function.

[0006] Inflammation is the body's immune response to injury or disease. Oxidative stress, including ultraviolet rays, reactive oxygen species, and free radicals, activates inflammatory factors, leading to various diseases and skin aging. Vasoactive polypeptides such as kinin, plasmin, and complement act to dilate and constrict blood vessels and induce chemotaxis. Other factors involved in the inflammatory response include lymphokines such as interleukin-6 (IL-6) and arachidonic acid. Arachidonic acid is metabolized via two pathways, cyclooxygenase and lipoxygenase, into the inflammatory mediators prostaglandins and leukotrienes, which mediate various inflammatory responses.

[0007] Anti-inflammatory agents are drugs that eliminate inflammation by eliminating the source of inflammation and reducing biological reactions and symptoms. Substances currently used for anti-inflammatory purposes include non-steroidal agents such as flufenamic acid, ibuprofen, benzydamine, and indomethacin, as well as steroidal agents such as prednisolone and dexamethasone. While allantoin, azulene, and hydrocortisone are known to have anti-inflammatory effects, these agents have limitations on their use due to safety concerns for the skin, or their efficacy is so minimal that they cannot be expected to substantially alleviate inflammation.

[0008] Hyaluronic acid, a type of glycosaminoglycan, is a chain-like polymeric polysaccharide composed of repeated linkages of glucuronic acid and N-acetylglucosamine residues. It has the ability to bind with large amounts of water to form a gel, giving it high viscosity and elasticity. Hyaluronic acid is a major component of the extracellular matrix and is reported to be involved in water retention, maintenance of intercellular spacing, storage and diffusion of cell growth factors and nutrients, as well as cell division, differentiation, and migration.

[0009] More than 50% of the hyaluronic acid in mammals is reported to be distributed in the skin, particularly in the intercellular spaces of the epidermis and the connective tissue of the dermis. This hyaluronic acid is known to be synthesized primarily by keratinocytes and fibroblasts. The amount of hyaluronic acid in human skin has been reported to decrease with aging, and this decrease in hyaluronic acid is thought to be one of the direct causes of the loss of skin elasticity and moisture content due to aging (BiochemBiophysActa 279, 265-275; Carbohydr Res 159, 127-136; Int J Dermatol 33, 119-122). Hyaluronic acid is also known to be involved in maintaining the structure of the stratum corneum and skin barrier function (J Cosmet Dermatol. 2007 Jun, 6(2), 75-82).

[0010] However, the hyaluronic acid that has the above-mentioned effect has a large molecular weight, so it is not well absorbed by the skin.In addition, currently, the method of injecting hyaluronic acid into the skin is performed, but this method not only causes a large irritation, but also the method of increasing the synthesis of hyaluronic acid in skin cells is more effective.Therefore, the research on the method of increasing the production of hyaluronic acid in the human body has been actively carried out, but the fact is that no noteworthy research results have been found yet.

[0011] On the other hand, reactive oxygen species (ROS), which enter the body from outside or are generated within the body, cause many problems, including accelerated aging and cancer. Therefore, much research and development has been conducted on antioxidants that inhibit oxidation caused by reactive oxygen species. Antioxidants are widely distributed in the animal and plant kingdoms, and many phenolic compounds, flavonoids, tocopherols, vitamin C, and selenium are known to be found in fruits and vegetables. However, naturally occurring antioxidants are not yet fully effective when applied to the skin. Therefore, inexpensive synthetic antioxidants with excellent antioxidant properties are widely used, but their use is limited due to safety concerns, such as side effects on the human body.

[0012] Meanwhile, the development of transportation has increased global mobility, resulting in the rapid spread of various infectious diseases and novel diseases. The indiscriminate use of antibacterial substances has led to the development of resistance in various microorganisms, posing a threat to humanity. In particular, there continues to be a demand for substances with antibacterial properties for direct cosmetic improvement or therapeutic treatment against microorganisms that may be harmful to the body, particularly for the preservation of perishable products (e.g., cosmetics, pharmaceuticals, or food).

[0013] Pesticides can be broadly divided into fungicides, insecticides, herbicides, and growth regulators. Fungicides are used to control crop damage caused by viruses and microorganisms. The excessive use of cheap and powerful synthetic antibacterial substances in the agricultural sector has come to threaten our dining tables. Various antibacterial substances are also used in everyday products that are used directly on the human body, and most of them are made from cheap chemical ingredients. Unfortunately, it has been scientifically proven that most of these antibacterial substances can be harmful to the human body if used over a long period of time.

[0014] Many products widely used in our daily lives, including food, medicines, household goods, and cosmetics, contain preservatives, which are antibacterial substances approved for human use, to prevent changes in their internal properties and preserve them for a certain period of time. In particular, cosmetics, which are applied by fingertip or frequently come into contact with the human body, can be susceptible to the intrusion of microorganisms from the outside, which can alter the quality of the product. Contamination of products used around the eyes, in particular, poses a risk of eye disease. In the United States in the 1920s, there was a case in which a consumer went blind after using mascara contaminated with microorganisms. Consequently, technologies have been developed to prevent microbial contamination of these products, ensure consumer safety, and preserve product quality.

[0015] Although some of the substances used as preservatives are naturally occurring, most are chemically synthesized artificial substances. Even parabens, which are the safest of the conventional preservatives and are widely used in household goods, cosmetics, and pharmaceuticals, have problems such as skin allergies (Andrea Counti et al., Contact Dermatitis, 1997, 37; 35-36), the possibility of them acting as environmental hormones (Edwin et al., Toxicology and applied pharmacology, 1998, 153; 12-19), and the induction of resistant bacteria.

[0016] Most natural active substances used as preservatives have not been commercialized due to issues such as color and odor, poor stability, narrow antibacterial spectrum, and dosage form issues. Only a few, such as hinokitiol, an extract from Japanese cypress, magnolol, an extract from magnolia, and DF-100, an extract from grapefruit, have been commercialized. Therefore, there is a growing need for naturally derived antibacterial substances that can replace existing synthetic chemical antibacterial substances. In particular, there is a need to develop natural antibacterial substances that have a broad antibacterial spectrum and dosage form stability while reducing the side effects that are the biggest drawback of antibacterial agents. Summary of the Invention [Problem to be solved by the invention]

[0017] Therefore, the inventors have confirmed that extracts of 109 kinds of plants have a skin whitening effect by reducing the total amount of melanin and the activity of tyrosinase in skin melanocytes, promote collagen synthesis in skin fibroblasts and inhibit collagenase activity, thereby promoting skin regeneration and improving skin elasticity or wrinkles, inhibit NO production and have anti-inflammatory or skin soothing effects, promote the production of hyaluronic acid in fibroblasts, thereby increasing the moisture content of the skin and having a moisturizing effect, scavenge free radicals and have an antioxidant effect, and also have a wide range of antibacterial effects against various bacteria, thereby completing the present invention.

[0018] Therefore, an object of the present invention is to provide a method for producing and using the phytoplankton of Island thistle (Cirsium nipponicum (Maxim.) Makino, English name: Island thistle), Codonopsis lanceolata, English name: Deodeok, Solidago virgaurea, English name: Ulleung goldenrod, Aster glehnii, English name: Ulleungdo aster), Allium victorialis, English name: Myeongyi, Allium senescens, English name: Aging chive, Rowan (Sorbus commixta, English name: Silvery mountain ash), Anthriscus sylvestris, English name: Wild chervil, Japanese knotweed (Fallopia sachalinensis, Giant Knotweed), Takeshima maple (Acer pictum, Mono maple), Takeshima ladyfern (Athyrium acutipinnulum, Ulleungdo ladyfern), Wasabi (Wasabia japonica, Wild wasabi), Yellow Mongolian snakegourd (Trichosanthes kirilowii, Yellow Mongolian snakegourd), Teshima polygonatum (Polygonatum odoratum, Pungdo solomon's seal), Hedera rhombea (Songak), Usan violet (Viola woosanensis, Ulleungdo sweet violet), Takeshima campanula (Campanula takesimana, English name: Korean bellflower), Takeshima lily (scientific name: Lilium hansonii, English name: Ulleungdo turk's-cap lily), Takeshima stag beetle (scientific name: Pseudolysimachion insulare, English name: Ulleungdo spike speedwell), Taigome (scientific name: Sedumoryzifolium, English name: Coastal moss-like stonecrop), Water chickweed (Scientific name: Stellaria aquatica, English name: Water chickweed, Giant chickweed), Houttuynia cordata Thunb., English name: Heartleaf Houttuynia), Hepatica maxima (Nakai) Nakai, English name: Ulleungdo liverleaf, Ranunculus quelpaertensis (H.Lev.) Nakai, Tricuspidate falsenettle (Scientific name: Boehmeria tricuspis (Hance) Makino), Dianthus longicalyx Miq., English name: Long-calyx Pink), Rumex crispus L., Curled dock, Hypericum erectum Thunb., Erect St. Johnsworth, Gynostemma pentaphylla (Thunb.) Makino, Five-leaf gynostemma, Bird's egg cucumber, Arabis takesimana Nakai, Ulleungdo rockcress, Sisymbrium officinale (L.) Scop., Hedge Mustard, Chimaphila japonica Miq., Asian mustard prince's pine), Ardisia japonica (Thunb.) Blume, English name: Marlberry, Hamabossu (Lysimachia mauritiana Lam., English name: Spoon-leaf yellow loosestrife), Climbing hydrangea (Hydrangea petiolarisSiebold & Zucc., English name: Climbing hydrangea), Sedum sarmentosum Bunge, English name: Stringy stonecrop, Sedum takesimense Nakai, English name: Ulleungdo stonecrop, Tiarella polyphylla D.Don, English name: Foam flower, Potentilla chinensis Ser., English name: East Asian cinquefoil, Amorpha fruticosa L., English name: Indigobush Amorpha, Lespedeza cuneata (Dum.Cours.) G.Don., English name: Sericea lespedeza, Medicago sativa L., English name: Alfalfa), Vicia amurensis Oett., English name: Amur vetch, Lythrum salicaria L., English name: Hairy purple loosestrife, Circaea mollis Siebold & Zucc., English name: South enchanter's nightshade, Epilobium pyrricholophum Franch. & Sav., English name: Long-seed willowherb, Oenothera biennis L., English name: Evening Primrose, Chrysosplenium flagelliferum F.Schmidt, English name: Stolon golden saxifrage, Saxifraga fortunei var. incisolobata (Engl. & Irmsch.) Nakai, English name: Serrate-petal rockfoil), Takeshima odonikosou (scientific name: Lamium takesimense NAKAI., English name: Ulleungdodeadnettle), Island ninebark (Physocarpus insularis (Nakai) Nakai, English name: Island ninebark), Corydalis filistipes Nakai, English name: Island corydalis, Rubus takesimensis Nakai, English name: Ulleungdo raspberry, Takeshima dwarf quince (Dystaenia takeshimana (Nakai) Kitag, English name: Korea Dystaenia), Takeshima violet (Viola takeshimana Nakai, English name: Ulleungdo violet), Euonymus japonicus Thunb., English name: Spindle Tree, Crimson grapevine (Vitis coignetiae Pulliat ex Planch., English name: Crimson grapevine), Udo (Aralia cordata var.continentalis (Kitag.) YCChu), Aralia elata (Miq.) Seem., English name: Japanese Angelica), Fatsia japonica (Thunb.) Decne. & Planch., English name: Glossy-leaf paper plant), Button vine (Clematis apiifolia DC., English name: Three-leaf clematis), Eupatorium tuberosum (Solanum lyratum Thunb., English name: Lyre-leaf nightshade), Ivy morning glory (Calystegia hederacea Wall., English name: Ivy morning glory), Sea bindweed (Calystegia soldanella (L.) Roem. & Schult., English name: Beach morning glory), Japanese bark bean (Callicarpa japonica Thunb., English name: East Asian beautyberry), Kawamidori (scientific name: Agastache rugosa (Fisch. & CAMey.)Kuntze (Korean mint), Wood laurel (Clinopodium chinense var. parviflorum (Kudo) H.Hara, English name: Small-flower Asian calamint), Henbit deadnettle (Lamium amplexicaule L., English name: Henbit deadnettle), Oriental motherwort (Leonurus japonicus Houtt., English name: Oriental motherwort), Long-stalk low meadow-rue (Thalictrum kemense Fr., English name: Long-stalk low meadow-rue), Korean spice viburnum (Viburnum carlesii Hemsl., English name: Korean spice viburnum), and Asian greater celandine (Chelidonium majus var. asiaticum (H.Hara) Ohwi, English name: Asian greater celandine), Asian aster (Aster spathulifolius Maxim., English name: Seashore spatulate aster), Japanese butterbur (Farfugium japonicum (L.) Kitam., English name: Leopard plant), Inula britannica var. japonica (Thunb.) Franch. & Sav., English name: Oriental yellowhead), Japanese butterbur (Petasites japonicus (Siebold & Zucc.) Maxim., English name: Giant butterbur), Takeshima sedge (Carex blepharicarpa Franch.), Japanese blue sedge (Carex breviculmis R.Br., English name: Short-stem sedge), and Miscanthus sinensis var. purpurascens (Andersson) Rendle, English name: Purple maiden silvergrass), Pennisetum alopecuroides (L.) Spreng., English name: Foxtailfountaingrass)、ヤダケ(Scientific name:Pseudosasa japonica (Siebold & Zucc.ex Steud.) Makino ex Nakai、English name:Arrow bamboo)、チシマザサ(Scientific name:Sasa kurilensis (Rupr.) Makino & Shibata、English name:Kuril bamboo)、クララ(Scientific name:Sophora f lavescens Aiton, English name: Shrubby sophora), ホンカンゾウ (Scientific name: Hemerocallis fulva (L.) L., English name: Orange Daylily), オニユリ (Scientific name: Lilium lancifolium Thunb., English name: Tiger lily), ヤブラン (Scientific name: Liriope platyphylla FTWang & T.Tang, English name: Big blue lilyturf), ダイオウマイヅルソウ (Scientific name: Maianthemum dilatatum (AWWood) A.Nelson & JFMacbr., English name: False lily of the valley), スギ (Scientific name: Cryptomeria japonica (Thunb.ex Lf) D.Don, English name: Japanese Cedar), Thunberg's bay-tree (Machilus thunbergii Siebold & Zucc., Thunberg's bay-tree), Himekomatsu (Pinus parviflora Siebold & Zucc., Ulleungdo white pine), Tsuga (Tsuga sieboldii Carriere, Ulleungdo hemlock), Silodame (Neolitsea sericea (Blume) Koidz., Sericeous newlitsea), Yuzuliha (Daphniphyllum macropodum Miq., Macropodous daphniphyllum), Mukunoki (Aphananthe aspera (Thunb.) Planch., Scabrous aphananthe)、エゾエノキ(Scientific name: Celtis jessoensis Koidz.、English name: Caudate-leaf hackberry)、オヒョウ(Scientific name: Ulmus laciniata (Trautv.) Mayr, English name: Manchurian Elm), Mountain alder (scientific name: Alnus maximowiczii Callier, English name: Montane alder), Takeshima linden (scientific name: Tilia insularis Nakai, English name: Ulleungdo linden), White laurel (scientific name: Styrax obassia Siebold & Zucc., English name: Fragrant snowbell), Takeshima cherry (scientific name: Prunus takesimensis Nakai, English name: Ulleungdo flowering cherry), Japanese laurel (scientific name: Aucuba japonica Thunb., English name: Spotted laurel), English name: Ulleungdo maple, Japanese yellow hawthorn (scientific name: Phellodendron insulare Nakai), Japanese holly (scientific name: Zanthoxylum The present invention provides a composition for skin whitening, skin regeneration, skin elasticity improvement, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant, or antibacterial effects, which contains, as an active ingredient, one or more plant extracts selected from the group consisting of Albizia julibrissin (Alianthus-like prickly ash), Ligustrum japonicum Thunb. (Ligustrum japonicum Thunb. (Wax-leaf privet)), Lonicera insularis Nakai (Ulleungdo honeysuckle)), Albizia julibrissin (Silk tree) and Eclipta prostrata (False daisy). [Means for solving the problem]

[0019] As a means for solving the above-mentioned problems, the present invention provides a method for producing a plant that is effective against a variety of plants, including island thistle (Cirsium nipponicum (Maxim.) Makino, English name: Island thistle), Codonopsis lanceolata, English name: Deodeok, Solidago virgaurea, English name: Ulleung goldenrod, Aster glehnii, English name: Ulleungdo aster), Allium victorialis, English name: Myeongyi, Allium senescens, English name: Aging chive, Rowan (Sorbus commixta, English name: Silvery mountain ash), and Anthriscus sylvestris, English name: Wild ash. chervil), Giant Knotweed (Fallopia sachalinensis, English name: Giant Knotweed), Mono maple (Acer pictum, English name: Mono maple), Ulleungdo ladyfern (Athyrium acutipinnulum, English name: Ulleungdo ladyfern), Wasabi (Wasabia japonica, English name: Wild wasabi), Yellow Mongolian snakegourd (Trichosanthes kirilowii, English name: Yellow Mongolian snakegourd), Polygonatum odoratum (Pungdo solomon's seal), Songak ivy (Hedera rhombea, English name: Songak), and Ulleungdo sweet violet (Viola woosanensis, English name: Ulleungdo sweet violet). violet), Takeshima bellflower (Campanula takesimana, English name: Korean bellflower), Takeshima lily (Lilium hansonii, English name: Ulleungdo turk's-cap lily), Takeshima stag beetle (Pseudolysimachion insulare, English name: Ulleungdo spike)speedwell), Coastal moss-like stonecrop (Sedum oryzifolium), Water chickweed (Stellaria aquatica), Houttuynia cordata Thunb., Heartleaf Houttuynia, Hepatica maxima (Nakai) Nakai, Ulleungdo liverleaf, Ranunculus quelpaertensis (H.Lev.) Nakai, Tricuspidate falsenettle (Boehmeria tricuspis (Hance) Makino), Dianthus longicalyx Miq., Long-calyx Pink), Rumex crispus L., Curled dock, Hypericum erectum Thunb., Erect St. Johnsworth, Gynostemma pentaphylla (Thunb.) Makino, Five-leaf gynostemma, Bird's egg cucumber, Arabis takesimana Nakai, Ulleungdo rockcress, Sisymbrium officinale (L.) Scop., Hedge Mustard, Chimaphila japonica Miq., Asian mustard prince's pine), Ardisia japonica (Thunb.) Blume, English name: Marlberry, and Hamabossu (Lysimachia mauritiana Lam., English name: Spoon-leaf yellow).loosestrife), climbing hydrangea (Hydrangea petiolaris Siebold & Zucc., English name: Climbing hydrangea), stringy stonecrop (Sedum sarmentosum Bunge, English name: Stringy stonecrop), Ulleungdo stonecrop (Sedum takesimense Nakai, English name: Ulleungdo stonecrop), Tiarella polyphylla D.Don, English name: Foam flower, Potentilla chinensis Ser., English name: East Asian cinquefoil, Indigobush amorpha (Amorpha fruticosa L., English name: Indigobush amorpha), Japanese bush clover (Lespedeza cuneata (Dum.Cours.) G.Don, English name: Sericea lespedeza), Medicago sativa L. (Alfalfa), Vicia amurensis Oett. (Amur vetch), Lythrum salicaria L. (Hairy purple loosestrife), Circaea mollis Siebold & Zucc. (South enchanter's nightshade), Epilobium pyrricholophum Franch. & Sav. (Long-seed willowherb), Oenothera biennis L. (Evening Primrose), Chrysosplenium flagelliferum F.Schmidt (Stolon golden) saxifrage), Serrate-petal rockfoil (scientific name: Saxifraga fortunei var. incisolobata (Engl. & Irmsch.) Nakai, English name: Serrate-petal rockfoil), Lamium takesimense (scientific name: Lamium takesimense)NAKAI., English name: Ulleungdo deadnettle), Physocarpus insularis (Nakai) Nakai, English name: Island ninebark, Corydalis filistipes Nakai, English name: Island corydalis, Rubus takesimensis Nakai, English name: Ulleungdo raspberry), Dystaenia takeshimana (Nakai) Kitag, English name: Korea Dystaenia, Viola takeshimana Nakai, English name: Ulleungdo violet, Euonymus japonicus Thunb., English name: Spindle Tree, Vitis coignetiae Pulliat ex Planch., English name: Crimson grapevine), Udo (Aralia cordata var.continentalis (Kitag.) YCChu), Aralia elata (Aralia elata (Miq.) Seem., English name: Japanese Angelica), Fatsia japonica (Thunb.) Decne. & Planch., English name: Glossy-leaf paper plant), Button vine (Clematis apiifolia DC., English name: Three-leaf clematis), Eupatorium tuberosum (Solanum lyratum Thunb., English name: Lyre-leaf nightshade), Ivy morning glory (Calystegia hederacea Wall., English name: Ivy morning glory), Beach bindweed (Calystegia soldanella (L.) Roem. & Schult., English name: Beach morning glory) glory), Callicarpa japonica Thunb. (East Asian beautyberry), Agastache rugosa (Fisch.& CA Mey.) Kuntze, English name: Korean mint), Wood laurel (Clinopodium chinense var. parviflorum (Kudo) H. Hara, English name: Small-flower Asian calamint), Henbit deadnettle (Lamium amplexicaule L., English name: Henbit deadnettle), Japanese motherwort (Leonurus japonicus Houtt., English name: Oriental motherwort), Japanese larch (Thalictrum kemense Fr., English name: Long-stalk low meadow-rue), Viburnum carlesii Hemsl., English name: Korean spice viburnum), Celandine (Chelidonium majus var. asiaticum (H. Hara) Ohwi, English name: Asian greater celandine), Asian aster (Aster spathulifolius Maxim., English name: Seashore spatulate aster), Japanese butterbur (Farfugium japonicum (L.) Kitam., English name: Leopard plant), Inula britannica var. japonica (Thunb.) Franch. & Sav., English name: Oriental yellowhead), Japanese butterbur (Petasites japonicus (Siebold & Zucc.) Maxim., English name: Giant butterbur), Takeshima sedge (Carex blepharicarpa Franch.), Japanese blue sedge (Carex breviculmis R.Br., English name: Short-stem sedge), and Miscanthus sinensis var. purpurascens (Andersson) Rendle, English name: Purple maiden silvergrass), Pennisetum alopecuroides (L.) Spreng., English name: Foxtailfountaingrass)、ヤダケ(Scientific name:Pseudosasa japonica (Siebold & Zucc.ex Steud.) Makino ex Nakai、English name:Arrow bamboo)、チシマザサ(Scientific name:Sasa kurilensis (Rupr.) Makino & Shibata、English name:Kuril bamboo)、クララ(Scientific name: Sophora flavescens Aiton, Shrubby sophora), Hemerocallis fulva (L.) L., Orange Daylily), Lilium lancifolium Thunb., Tiger lily), Liriope platyphylla FTWang & T.Tang, Big blue lilyturf), Maianthemum dilatatum (AWWood) A.Nelson & JFMacbr., False lily of the valley), Cryptomeria japonica (Thunb.ex Lf) D.Don, Japanese lily Cedar), Thunberg's bay-tree (Machilus thunbergii Siebold & Zucc., Thunberg's bay-tree), Himekomatsu (Pinus parviflora Siebold & Zucc., Ulleungdo white pine), Tsuga (Tsuga sieboldii Carriere, Ulleungdo hemlock), Silodame (Neolitsea sericea (Blume) Koidz., Sericeous newlitsea), Yuzuliha (Daphniphyllum macropodum Miq., Macropodous daphniphyllum), Mukunoki (Aphananthe aspera (Thunb.) Planch., Scabrous aphananthe)、エゾエノキ(Scientific name: Celtis jessoensis Koidz.、English name: Caudate-leaf hackberry)、オヒョウ(Scientific name: Ulmus laciniata (Trautv.) Mayr, English name: Manchurian Elm), Mountain alder (scientific name: Alnus maximowiczii Callier, English name: Montane alder), Takeshima linden (scientific name: Tilia insularis Nakai, English name: Ulleungdo linden), White laurel (scientific name: Styrax obassia Siebold & Zucc., English name: Fragrant snowbell), Takeshima cherry (scientific name: Prunus takesimensis Nakai, English name: Ulleungdo flowering cherry), Japanese laurel (scientific name: Aucuba japonica Thunb., English name: Spotted laurel), English name: Ulleungdo maple, Japanese yellow hawthorn (scientific name: Phellodendron insulare Nakai), Japanese holly (scientific name: Zanthoxylum The present invention provides a composition for skin whitening, skin regeneration, skin elasticity improvement, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant or antibacterial effects, which contains one or more plant extracts selected from the group consisting of Albizia julibrissin (Alianthus-like prickly ash), Ligustrum japonicum Thunb. (Ligustrum japonicum Thunb. (Wax-leaf privet)), Lonicera insularis Nakai (Ulleungdo honeysuckle)), Albizia julibrissin (Silk tree) and Eclipta prostrata (False daisy). [Effects of the Invention]

[0020] The composition containing the plant extract of the present invention has a skin whitening effect by reducing the total amount of melanin and the activity of tyrosinase in skin melanocytes, promotes collagen synthesis in skin fibroblasts and inhibits collagenase activity, thereby promoting skin regeneration and improving skin elasticity or wrinkles, inhibits NO production and has an anti-inflammatory or skin soothing effect, promotes the production of hyaluronic acid in fibroblasts, thereby increasing the moisture content of the skin and having a moisturizing effect, scavenges free radicals and has an antioxidant effect, and also exhibits a wide range of antibacterial effects against various bacteria, making it suitable for use as a cosmetic composition, pharmaceutical composition, topical skin preparation, or food composition. DETAILED DESCRIPTION OF THE INVENTION

[0021] The configuration of the present invention will be specifically described below.

[0022] The present invention relates to a variety of plants, including island thistle (Cirsium nipponicum (Maxim.) Makino, English name: Island thistle), Codonopsis lanceolata (Deodeok, English name: Deodeok), Solidago virgaurea (Ulleung goldenrod, English name: Ulleung goldenrod), Aster glehnii (Ulleungdo aster), Allium victorialis (Myeongyi, English name: Myeongyi), Aging chive (Allium senescens, English name: Aging chive), Rowan (Sorbus commixta, English name: Silvery mountain ash), Wild chervil (Anthriscus sylvestris, English name: Wild chervil), and Japanese knotweed (Fallopia sachalinensis, Giant Knotweed), Takeshima maple (Acer pictum, Mono maple), Takeshima ladyfern (Athyrium acutipinnulum, Ulleungdo ladyfern), Wasabi (Wasabia japonica, Wild wasabi), Yellow Mongolian snakegourd (Trichosanthes kirilowii, Yellow Mongolian snakegourd), Teshima polygonatum (Polygonatum odoratum, Pungdo solomon's seal), Hedera rhombea (Songak), Usan violet (Viola woosanensis, Ulleungdo sweet violet), Takeshima campanula (Campanula takesimana, English name: Korean bellflower), Takeshima lily (scientific name: Lilium hansonii, English name: Ulleungdo turk's-cap lily), Takeshima stag beetle (scientific name: Pseudolysimachion insulare, English name: Ulleungdo spike speedwell), Taigome (scientific name: Sedumoryzifolium, English name: Coastal moss-like stonecrop), Water chickweed (Scientific name: Stellaria aquatica, English name: Water chickweed, Giant chickweed), Houttuynia cordata Thunb., English name: Heartleaf Houttuynia), Hepatica maxima (Nakai) Nakai, English name: Ulleungdo liverleaf, Ranunculus quelpaertensis (H.Lev.) Nakai, Tricuspidate falsenettle (Scientific name: Boehmeria tricuspis (Hance) Makino), Dianthus longicalyx Miq., English name: Long-calyx Pink), Rumex crispus L., Curled dock, Hypericum erectum Thunb., Erect St. Johnsworth, Gynostemma pentaphylla (Thunb.) Makino, Five-leaf gynostemma, Bird's egg cucumber, Arabis takesimana Nakai, Ulleungdo rockcress, Sisymbrium officinale (L.) Scop., Hedge Mustard, Chimaphila japonica Miq., Asian mustard prince's pine), Ardisia japonica (Thunb.) Blume, English name: Marlberry, Hamabossu (Lysimachia mauritiana Lam., English name: Spoon-leaf yellow loosestrife), Climbing hydrangea (Hydrangea petiolarisSiebold & Zucc., English name: Climbing hydrangea), Sedum sarmentosum Bunge, English name: Stringy stonecrop, Sedum takesimense Nakai, English name: Ulleungdo stonecrop, Tiarella polyphylla D.Don, English name: Foam flower, Potentilla chinensis Ser., English name: East Asian cinquefoil, Amorpha fruticosa L., English name: Indigobush Amorpha, Lespedeza cuneata (Dum.Cours.) G.Don., English name: Sericea lespedeza, Medicago sativa L., English name: Alfalfa), Vicia amurensis Oett., English name: Amur vetch, Lythrum salicaria L., English name: Hairy purple loosestrife, Circaea mollis Siebold & Zucc., English name: South enchanter's nightshade, Epilobium pyrricholophum Franch. & Sav., English name: Long-seed willowherb, Oenothera biennis L., English name: Evening Primrose, Chrysosplenium flagelliferum F.Schmidt, English name: Stolon golden saxifrage, Saxifraga fortunei var. incisolobata (Engl. & Irmsch.) Nakai, English name: Serrate-petal rockfoil), Takeshima odonikosou (scientific name: Lamium takesimense NAKAI., English name: Ulleungdodeadnettle), Island ninebark (Physocarpus insularis (Nakai) Nakai, English name: Island ninebark), Corydalis filistipes Nakai, English name: Island corydalis, Rubus takesimensis Nakai, English name: Ulleungdo raspberry, Takeshima dwarf quince (Dystaenia takeshimana (Nakai) Kitag, English name: Korea Dystaenia), Takeshima violet (Viola takeshimana Nakai, English name: Ulleungdo violet), Euonymus japonicus Thunb., English name: Spindle Tree, Crimson grapevine (Vitis coignetiae Pulliat ex Planch., English name: Crimson grapevine), Udo (Aralia cordata var.continentalis (Kitag.) YCChu), Aralia elata (Miq.) Seem., English name: Japanese Angelica), Fatsia japonica (Thunb.) Decne. & Planch., English name: Glossy-leaf paper plant), Button vine (Clematis apiifolia DC., English name: Three-leaf clematis), Eupatorium tuberosum (Solanum lyratum Thunb., English name: Lyre-leaf nightshade), Ivy morning glory (Calystegia hederacea Wall., English name: Ivy morning glory), Sea bindweed (Calystegia soldanella (L.) Roem. & Schult., English name: Beach morning glory), Japanese bark bean (Callicarpa japonica Thunb., English name: East Asian beautyberry), Kawamidori (scientific name: Agastache rugosa (Fisch. & CAMey.)Kuntze (Korean mint), Wood laurel (Clinopodium chinense var. parviflorum (Kudo) H.Hara, English name: Small-flower Asian calamint), Henbit deadnettle (Lamium amplexicaule L., English name: Henbit deadnettle), Oriental motherwort (Leonurus japonicus Houtt., English name: Oriental motherwort), Long-stalk low meadow-rue (Thalictrum kemense Fr., English name: Long-stalk low meadow-rue), Korean spice viburnum (Viburnum carlesii Hemsl., English name: Korean spice viburnum), and Asian greater celandine (Chelidonium majus var. asiaticum (H.Hara) Ohwi, English name: Asian greater celandine), Asian aster (Aster spathulifolius Maxim., English name: Seashore spatulate aster), Japanese butterbur (Farfugium japonicum (L.) Kitam., English name: Leopard plant), Inula britannica var. japonica (Thunb.) Franch. & Sav., English name: Oriental yellowhead), Japanese butterbur (Petasites japonicus (Siebold & Zucc.) Maxim., English name: Giant butterbur), Takeshima sedge (Carex blepharicarpa Franch.), Japanese blue sedge (Carex breviculmis R.Br., English name: Short-stem sedge), and Miscanthus sinensis var. purpurascens (Andersson) Rendle, English name: Purple maiden silvergrass), Pennisetum alopecuroides (L.) Spreng., English name: FoxtailFountaingrass), ヤダケ(Scientific name: Pseudosasa japonica (Siebold & Zucc.ex Steud.) Makino ex Nakai, English name: Arrow bamboo), チシマザサ(Scientific name: Sasa kurilensis (Rupr.) Makino & Shibata, English name: Kuril bamboo), クララ(Scientific name: Sophora flavescens Aiton, British name: Shrubby sophora), ホンカンゾウ (scientific name: Hemerocallis fulva (L.) L., British name: Orange Daylily), オニユリ (scientific name: Lilium lancifolium Thunb., British name: Tiger lily), ヤブラン (scientific name: Liriope platyphylla FTWang & T.Tang, British name: Big blue lilyturf), ダイオウマイヅルソウ (scientific name: Maianthemum dilatatum (AWWood) A.Nelson & JFMacbr., British name: False lily of the valley), スギ(scientific name: Cryptomeria japonica (Thunb.ex Lf) D.Don, English name: Japanese Cedar), タブノキ(scientific name: Machilus thunbergii Siebold & Zucc., English name: Thunberg's bay-tree), Himekomatsu (Scientific name: Pinus parviflora Siebold & Zucc., English name: Ulleungdo white pine), Tsuga (Scientific name: Tsuga sieboldii Carriere, English name: Ulleungdo hemlock), Silodame (Scientific name: Neolitsea sericea (Blume) Koidz., English name: Sericeous newlitsea), Yuzuliha (Scientific name: Daphniphyllum macropodum Miq., English name: Macropodous daphniphyllum), Mukunoki (Scientific name: Aphananthe aspera (Thunb.) Planch., English name: Scabrous aphananthe)、エゾエノキ(Scientific name: Celtis jessoensis Koidz.、English name: Caudate-leaf hackberry)、オヒョウ(Scientific name: Ulmus laciniata (Trautv.) Mayr, English name: Manchurian Elm), Mountain alder (scientific name: Alnus maximowiczii Callier, English name: Montane alder), Takeshima linden (scientific name: Tilia insularis Nakai, English name: Ulleungdo linden), White laurel (scientific name: Styrax obassia Siebold & Zucc., English name: Fragrant snowbell), Takeshima cherry (scientific name: Prunus takesimensis Nakai, English name: Ulleungdo flowering cherry), Japanese laurel (scientific name: Aucuba japonica Thunb., English name: Spotted laurel), English name: Ulleungdo maple, Japanese yellow hawthorn (scientific name: Phellodendron insulare Nakai), Japanese holly (scientific name: Zanthoxylum The present invention relates to a composition containing, as an active ingredient, one or more plant extracts selected from the group consisting of Albizia julibrissin (Alianthus-like prickly ash), Ligustrum japonicum Thunb. (Ligustrum japonicum Thunb. (Wax-leaf privet), Lonicera insularis Nakai (Ulleungdo honeysuckle), Albizia julibrissin (Silk tree), and Eclipta prostrata (False daisy).

[0023] The composition containing the plant extract according to the present invention as an active ingredient can be used as a skin whitening, skin regeneration, skin elasticity improvement, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant and / or antibacterial composition. The antibacterial composition can be used as an oral antibacterial composition, a composition for treating tooth decay, a composition for treating periodontal disease, or a composition for treating bad breath.

[0024] As can be seen from the examples of the present invention, compositions containing the plant extract of the present invention as an active ingredient exhibit significantly superior skin whitening, skin regeneration, skin elasticity improvement, skin wrinkle reduction, anti-inflammatory, skin soothing, moisturizing, antioxidant and / or antibacterial effects even at low concentrations, and can therefore be used as active ingredients in cosmetics, pharmaceuticals, topical skin preparations and food compositions for skin whitening, skin regeneration, skin elasticity improvement, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant and / or antibacterial purposes.

[0025] In the present invention, the type of plant is not limited, and plants can be used regardless of their origin, such as cultivated plants or commercially purchased plants.

[0026] The plant extract of the present invention may be extracted by any method known in the art, and the method is not particularly limited. Alternatively, commercially available extracts may be used.

[0027] The plant extract according to the present invention can have skin whitening, skin regeneration, improvement of skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant and / or antibacterial effects regardless of the region. Preferably, the plant extract collected and extracted from Ulleungdo Island can have even better skin whitening, skin regeneration, improvement of skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant and / or antibacterial effects.

[0028] The plant extract may be prepared from any part of the plant, and is not limited to the part from which it is extracted. The preparation of the plant extract is not limited by the form of the plant, and the plant may be any plant that has been processed, such as dried. For example, the plant in the present invention may be the whole plant, roots, stems, leaves, fruits, flowers, buds, branches, bark, sap, propagules, and / or seeds of the aforementioned plants.

[0029] The term "extract" as used herein includes the extract itself and all formulations that can be made using the extract, such as the extract obtained by extracting the aforementioned plants, diluted or concentrated solutions of the extract, dried products obtained by drying the extract, crude or purified products of the extract, fermented products of the extract, and mixtures thereof. It also includes juice obtained by directly squeezing or crushing the plant and then filtering. The aforementioned plants can be extracted as they are or by being processed. "Processing" refers to a pharmaceutical technique that changes the inherent properties of medicinal ingredients by processing them according to traditional Chinese medicine theory. Examples include the "frying" method, in which medicinal ingredients are fried; the "roasting" method, in which medicinal ingredients are fried with a certain amount of liquid auxiliary ingredients to allow the auxiliary ingredients to permeate the medicinal tissue; and the "steaming" method, in which liquid auxiliary ingredients are mixed according to the processing instructions for each medicinal ingredient, heated and steamed in an appropriate container, or steamed to a certain degree and then dried.

[0030] In the present invention, the extraction method is not particularly limited and may be a method commonly used in the art, including, but not limited to, solvent extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., which may be performed alone or in combination of two or more.

[0031] In the present invention, the type of extraction solvent used for extraction is not particularly limited, and any solvent known in the art can be used. In the present invention, the extract can be obtained by extraction with water, a lower alcohol having 1 to 6 carbon atoms, or a mixture thereof. Non-limiting examples of the extraction solvent include water; a lower alcohol having 1 to 6 carbon atoms, such as methanol, ethanol, propyl alcohol, or butyl alcohol; a polyhydric alcohol, such as glycerin, butylene glycol, or propylene glycol; a hydrocarbon solvent, such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, or dichloromethane; or a mixture thereof. Specifically, water, a lower alcohol, 1,3-butylene glycol, or ethyl acetate can be used alone or in combination. When two or more solvents are used in combination, the mixing ratio of the solvents is not particularly limited.

[0032] In the present invention, the extraction may be performed using a solvent in an amount of 1 to 100 times, specifically 1 to 50 times, more specifically 2 to 20 times the weight of the dried plant material, at an extraction temperature of 10 to 80°C, specifically 15 to 50°C, for an extraction time of 2 hours to 30 days, specifically 12 hours to 18 days, and may include a process of extracting the dried and crushed plant material one to five times in succession to obtain a liquid crude extract.

[0033] In the present invention, the extract may be filtered to remove suspended solid particles, for example, by filtering particles using nylon, filter paper, or the like, or by freeze filtration, and then used as is, or may be dried using freeze drying, hot air drying, spray drying, or the like.

[0034] The liquid crude extract can be separated from the dried, crushed plant material by methods such as vacuum filtration and then concentrated or dried. For example, the liquid crude extract can be concentrated under reduced pressure at 20 to 100°C, preferably 30 to 70°C, using a vacuum rotary evaporator to obtain a concentrated liquid, or the liquid extract can be dried to obtain a powdered extract. The concentrated or powdered extract can be dissolved in water, alcohol, DMSO (dimethyl sulfoxide), or a mixture thereof, as needed, before use.

[0035] In the present invention, the active ingredient may be a fraction of a plant extract.

[0036] As used herein, the term "fractionate" refers to the product obtained by fractionation to separate a specific component or group of specific components from a mixture containing various components.

[0037] In the present invention, the fractionation method for obtaining the fraction is not particularly limited and may be a method commonly used in the art, including, but not limited to, a method in which a plant extract obtained by extracting a plant is treated with a predetermined solvent to obtain a fraction from the extract.

[0038] In the present invention, the type of solvent used to obtain the fraction is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol; and non-polar solvents such as hexane, ethyl acetate, chloroform, dichloromethane, and butanol. These solvents may be used alone or in combination. When an alcohol is used as the fractionation solvent, an alcohol having 1 to 6 carbon atoms can be used.

[0039] In the present invention, the term "whitening effect" refers not only to the inhibition of melanin synthesis to lighten the skin tone, but also to all actions such as suppressing or preventing melanin deposition in the skin (hyperpigmentation), and alleviating or improving spots and freckles caused by ultraviolet rays, hormones, or genetics.

[0040] In the present invention, the term "skin regeneration effect" refers to the recovery of skin tissue from damage caused by external and internal factors by promoting the activity of skin stem cells. Examples of damage caused by external factors include ultraviolet rays, external pollutants, wounds, and trauma, and examples of damage caused by internal factors include stress.

[0041] In the present invention, the term "elasticity improving effect" refers to reducing the degree of sagging or loosening of the skin. Furthermore, the term "elasticity" refers to maintaining the skin's elasticity in a state where elastin and collagen are sufficiently present.

[0042] In the present invention, the term "wrinkle-improving effect" refers to suppressing or inhibiting the formation of wrinkles on the skin, or alleviating wrinkles that have already formed.

[0043] In the present invention, "anti-inflammatory effect" refers to the suppression of inflammation, a complex pathological response of biological tissues to a certain stimulus, involving three simultaneous events: tissue degeneration, circulatory disorders and exudation, and tissue proliferation. More specifically, inflammation is part of innate immunity. Similar to other animals, human innate immunity recognizes cell surface patterns specific to pathogens. Phagocytes recognize cells with such surfaces as non-self and attack the pathogens. When pathogens penetrate the body's physical barriers, an inflammatory response occurs. The inflammatory response is a nonspecific defense mechanism that creates a hostile environment for invading microorganisms at the wound site. During an inflammatory response, when a wound is created or an external infectious agent enters the body, white blood cells, responsible for the initial immune response, gather together and express cytokines. Therefore, the level of cytokine expression within cells is an indicator of the activation of the inflammatory response. Examples of skin diseases associated with inflammation include, but are not limited to, atopic dermatitis, psoriasis, erythematous diseases caused by radiation, chemicals, burns, etc., acid burns, bullous skin diseases, lichenoid diseases, pruritus caused by allergies, seborrheic eczema, rosacea, pemphigus vulgaris, erythema multiforme exudative, erythema nodosum, balanitis, vulvitis, inflammatory alopecia such as alopecia areata, cutaneous T-cell lymphoma, etc. The cosmetic composition having an anti-inflammatory effect has a skin soothing effect in addition to suppressing inflammation.

[0044] In the present invention, the term "moisturizing effect" refers to increasing the moisture content of the skin, reducing roughness of the skin, and maintaining a moist state, and refers to all actions such as supplying moisture to the skin and blocking moisture evaporation to maintain skin flexibility, and inducing uniform exfoliation of keratin to maintain a smooth surface. When the moisturizing effect of the skin is enhanced, it can have a beneficial effect on improving skin elasticity and improving skin wrinkles.

[0045] In the present invention, the term "antioxidant effect" refers to the inhibition of cellular oxidation caused by highly reactive free radicals or reactive oxygen species (ROS) due to oxidative stress caused by intracellular metabolism or ultraviolet light, and includes the elimination of free radicals or reactive oxygen species to reduce the resulting damage to cells.

[0046] In the present invention, the term "antibacterial effect" refers to the ability to resist bacteria, and includes all mechanisms that act to protect against the action of microorganisms such as bacteria, mold, yeast, etc. Furthermore, in the present invention, the antibacterial effect may refer to the inhibition of the growth of oral bacteria, and the bacteria may be bacteria of the genus Streptococcus or Porphyromonas, etc., and specifically may be selected from the group consisting of Streptococcus mutans, Streptococcus sanguis, Streptococcus sanguinis, Streptococcus salivarius subsp. thermophilus, and Porphyromonas gingivalis.

[0047] In the present invention, "effective amount" or "containing as an active ingredient" refers to the amount of extract that exhibits a whitening effect, promotes the regeneration of damaged skin, reduces wrinkles, suppresses inflammation, moisturizes, inhibits or alleviates cellular oxidation, and has a broad antibacterial effect against various bacteria. When the composition of the present invention contains an effective amount of the plant extract, it can provide desirable skin whitening, skin regeneration, skin elasticity improvement, wrinkle reduction, anti-inflammatory, skin soothing, moisturizing, antioxidant, and antibacterial effects.

[0048] In the cosmetic composition, pharmaceutical composition, topical skin preparation and food composition according to the present invention, the content of the plant extract is preferably 0.0001 to 10 parts by weight based on the total weight of the cosmetic composition, pharmaceutical composition, topical skin preparation and food composition.

[0049] The present invention provides a cosmetic composition containing a plant extract as an active ingredient for skin whitening, skin regeneration, improvement of skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant and / or antibacterial purposes.

[0050] When the composition is used as a cosmetic composition, it may be prepared in the form of a general emulsifier or solubilizer. For example, it may be in the form of a lotion such as a softening lotion or a nourishing lotion; an emulsion such as a facial lotion or a body lotion; a cream such as a nourishing cream, a moisturizing cream, or an eye cream; an essence; a cosmetic ointment; a spray; a gel; a pack; a sunscreen; a makeup base; a liquid, solid, or spray type foundation; a powder; a makeup remover such as a cleansing cream, a cleansing lotion, or a cleansing oil; or a cleanser such as a cleansing foam, a soap, or a body wash.

[0051] In addition to the plant extract, the cosmetic composition may contain additives commonly used in the cosmetic field, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic emulsifiers, nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, moisturizing agents, essential oils, dyes, pigments, hydrophilic active agents, lipophilic active agents, or lipid vesicles. The additives may include both natural and synthetic additives.

[0052] The natural additives refer to ingredients such as organic raw materials, plants and plant-derived raw materials, animals and animal-derived raw materials, minerals and mineral-derived raw materials, and water. For example, the natural additives may be one or more additives selected from the group consisting of moisturizers, UV-blocking agents, neutralizing agents, fragrances, preservatives, antioxidants, thickeners, viscosity adjusters, film-forming agents, and pigments.

[0053] In the present invention, the natural additives include natural products or ingredients obtained from natural substances, or ingredients obtained by simply modifying natural products or ingredients derived from natural substances, or ingredients derived from natural substances that are synthesized, but exclude synthetic ingredients that are not naturally derived ingredients and are synthesized by artificial methods.

[0054] The natural raw materials may refer to, for example, cosmetic raw materials that meet national organic standards, eco-certified grades, or equivalent natural grades.

[0055] Eco-certification standards refer to compositions made from ingredients grown and processed in an environmentally friendly manner, eliminating synthetic processes. Representative eco-certification standards include France's Ecocert, Europe's Cosmos, the US Department of Agriculture (USDA), Germany's Association of German Industries and Trading Firms (BDIH), and Japan's Japanese Association of Standards (JAS). To meet the needs of consumers who prefer eco-friendly products, various countries are developing eco-certification systems to certify the safety of ingredients or products. While each country has its own specific specifications and scope, the overall framework for the primary components of raw materials and ingredients is consistent globally. Natural ingredients are classified as eco-friendly organic ingredients, while other semi-processed ingredients are classified as PPAI (Physically Processed Agro Ingredients) and CPAI (Chemically Processed Agro Ingredients). Only ingredients that meet these standards are certified by Ecocert, Cosmos, or USDA.

[0056] In the present invention, nationally certified organic and eco-certified raw materials and ingredients refer to cosmetic raw materials and compositions that are composed only of ingredients that comply with or can comply with national organic standards and eco-certification standards and that can meet the national organic and eco-grade standards, for example, those that are composed only of ingredients that comply with or can comply with standards set by the Chinese government or organic and eco-certification standards recognized in China.

[0057] In the present invention, the synthetic additive means a raw material that is not naturally derived but is chemically synthesized.

[0058] When the plant extract is commercialized as a cosmetic product, a relatively high concentration of the plant extract may be contained in wash-off cosmetics, such as makeup removers or cleansers, in which the active ingredients remain on the skin for a short period of time. On the other hand, a lower concentration of the plant extract may be contained in leave-on cosmetics, such as lotions, emulsions, creams, or essences, in which the active ingredients remain on the skin for a long period of time. While not limited thereto, in one embodiment of the present invention, the composition may contain 0.0001 to 10 parts by weight (preferably 0.0001 to 5 parts by weight) of the plant extract based on the total weight of the composition. If the composition of the present invention contains less than 0.0001 parts by weight of the plant extract, sufficient skin whitening, skin regeneration, skin elasticity improvement, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant, and / or antibacterial effects may not be achieved. If the composition of the present invention contains more than 10 parts by weight of the plant extract, undesirable reactions such as allergies may occur or skin safety issues may arise.

[0059] Each of the above-mentioned components contained in the cosmetic composition of the present invention can be preferably contained in the cosmetic composition of the present invention within a range not exceeding the maximum usage amount specified in the "Cosmetic Safety and Technical Standards" established by the Chinese government.

[0060] The present invention also provides a pharmaceutical composition for skin whitening, skin regeneration, improvement of skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant and / or antibacterial properties, which contains the plant extract as an active ingredient.

[0061] The term "pharmaceutical composition" used in the present invention can be used as a concept including the meaning of "quasi-drug" or "drug."

[0062] In one embodiment, skin whitening can refer to the prevention or treatment of skin hyperpigmentation, anti-inflammatory or moisturizing can refer to the prevention or treatment of dry skin diseases including atopy or psoriasis, and antioxidant can refer to the scavenging of active oxygen.

[0063] The pharmaceutical compositions may be in the form of a solution, suspension or emulsion in oily or aqueous media, or in the form of extracts, powders, granules, tablets or capsules.

[0064] The composition may also contain one or more additional active ingredients exhibiting the same or similar functions. For example, known ingredients may be included for skin whitening, skin regeneration, improving skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant, and / or antibacterial purposes. When additional ingredients for skin whitening, skin regeneration, improving skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant, and / or antibacterial purposes are included, the skin whitening, skin regeneration, improving skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant, and / or antibacterial effects of the composition of the present invention may be further enhanced. When adding such ingredients, consideration should be given to skin safety when used in combination, ease of formulation, and stability of the active ingredients. In one embodiment of the present invention, the composition may further comprise one or more ingredients selected from the group consisting of skin whitening ingredients known in the art, such as substances that inhibit the activity of tyrosinase enzymes, such as kojic acid and arbutin, hydroquinone, vitamin C and derivatives thereof, and various plant extracts. The additional ingredients may be included in an amount of 0.0001 to 10 parts by weight based on the total weight of the composition, and the range of the content may be adjusted depending on factors such as skin safety and ease of formulation of the plant extract.

[0065] Additionally, the composition of the present invention may further comprise a pharmaceutically acceptable carrier.

[0066] Pharmaceutically acceptable carriers can contain a variety of ingredients such as buffers, sterile water for injection, normal saline or phosphate buffered saline, sucrose, histidine, salts, polysorbates, and the like.

[0067] The composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical formulation, for example, in various oral and parenteral dosage forms at the time of clinical administration. When formulated, the composition can be prepared using a diluent or excipient such as a commonly used filler, extender, binder, wetting agent, disintegrant, surfactant, etc.

[0068] Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations may be prepared by mixing the pharmaceutical composition of the present invention with at least one or more excipients, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc.

[0069] In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, oral solutions, emulsions, syrups, etc., and in addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, flavoring agents, preservatives, etc. may also be included.

[0070] Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. Suppository bases may include witepsol, macrogol, Tween 61, cocoa butter, laurin butter, and glycerogelatin.

[0071] The effective amount of the plant extract contained in the composition of the present invention may vary depending on the form in which the composition is formulated, the method by which the plant extract is applied to the skin, and the time the plant extract remains on the skin. For example, when the composition is formulated in a pharmaceutical dosage form, the plant extract may be contained in a higher concentration than when the composition is formulated as a cosmetic product for daily application to the skin. Therefore, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, and more preferably 50 to 60 mg / kg, based on the amount of the plant extract, and may be administered 1 to 6 times a day.

[0072] The compositions of the present invention can be used alone or in combination with surgery, radiation therapy, hormone therapy, chemotherapy and methods using biological response modifiers.

[0073] In addition, compositions for skin whitening, skin regeneration, improvement of skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant and / or antibacterial purposes, which contain the plant extract of the present invention as an active ingredient, can be provided as quasi-drugs.

[0074] In the present invention, the "quasi-drug" may contain the plant extract as an active ingredient and, if necessary, further contain a pharmaceutically acceptable carrier, excipient, or diluent. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effects of the present invention, and may include, for example, but is not limited to, a filler, extender, binder, wetting agent, disintegrant, surfactant, lubricant, sweetener, flavoring agent, or preservative.

[0075] The quasi-drug may be, for example, a disinfectant, a shower foam, an ointment, a wet tissue, a coating agent, etc., and preferably may be prepared as a semi-solid preparation such as an external ointment, a lotion, etc., but is not limited thereto. The formulation method, dosage, method of use, components, etc. of the quasi-drug may be appropriately selected from conventional techniques known in the art.

[0076] The present invention also provides an external skin preparation for skin whitening, skin regeneration, improvement of skin elasticity, wrinkle reduction, anti-inflammatory, skin soothing, skin moisturizing, antioxidant and / or antibacterial purposes, which contains the plant extract as an active ingredient.

[0077] When the plant extract is used as an external preparation for the skin, it may additionally contain adjuvants commonly used in the field of dermatology, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, moisturizing agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles, or any other ingredients commonly used in external preparations for the skin, and these ingredients may be incorporated in amounts commonly used in the field of dermatology.

[0078] When the plant extract is provided in the form of an external skin preparation, it may have a dosage form such as, but not limited to, an ointment, a patch, a gel, a cream, or a spray.

[0079] The present invention also relates to a food composition for skin whitening, skin regeneration, improving skin elasticity, reducing wrinkles, anti-inflammatory, skin soothing, skin moisturizing, antioxidant and / or antibacterial properties, which comprises the plant extract.

[0080] When the plant extract of the present invention is provided as a food composition, the composition may contain, in addition to the active ingredient, a food-safe food supplement additive.

[0081] The food supplement additives refer to components that can be added supplementarily to foods and are added to produce various forms of health functional foods, and can be appropriately selected and used by those skilled in the art. Examples of food supplement additives include, but are not limited to, various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants, fillers, pectinic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonation agents used in carbonated beverages.

[0082] The food composition may also include functional health foods. In the present invention, "functional health foods" refers to foods that have been added with added value using physical, biochemical, or bioengineering techniques to act and express the functions of the food for a specific purpose, or foods that have been designed and processed to fully express the bioregulatory functions of the food composition, such as regulating the body's defense rhythm and preventing and recovering from diseases. Health supplement foods have more active health maintenance and promotion effects than general foods, and health supplement foods refer to foods intended for health supplementation. In some cases, the terms "functional food," "health food," and "health supplement" are used interchangeably.

[0083] Specifically, the health functional foods are foods prepared by adding the plant extract of the present invention to food ingredients such as beverages, teas, spices, gum, and confectioneries, or by encapsulating, powdering, or preparing a suspension, etc., and when ingested, they bring about specific health benefits. However, unlike general medicines, because they are made from food ingredients, they have the advantage of not causing side effects that can occur when taking medicines for a long period of time.

[0084] The food may contain nutrient-acceptable food supplement additives, and may further contain suitable carriers, excipients and diluents that are commonly used in the manufacture of health functional foods.

[0085] The composition may contain additional ingredients commonly used in food compositions to improve odor, taste, and visual appearance. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, and panthotenic acid may be included. Minerals such as zinc (Zn), iron (Fe), calcium (Ca), magnesium (Mg), manganese (Mn), copper (Cu), and chromium (Cr) may also be included. Amino acids such as lysine, tryptophan, cysteine, and valine may also be included. The health functional food of the present invention may take any form and may include any food commonly defined, and may be interchangeably referred to as functional food or other terms known in the art.

[0086] In addition, the health functional food of the present invention can be prepared by mixing other appropriate supplementary ingredients and known additives that can be contained in foods according to the selection of those skilled in the art. Examples of foods to which the plant extract of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gum, dairy products including ice cream, various soups, beverages, tea, health supplements, alcoholic beverages, and vitamin complexes. The plant extract of the present invention can be added to soups, teas, jellies, and juices made from the plant extract of the present invention as a main ingredient. Foods used as animal feed are also included.

[0087] The present invention will be described in detail below with reference to examples. However, the following examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention.

[0088] Production Example 1: Production of plant extracts

[0089] The whole plant, roots, stems, leaves, fruits, flowers, buds, branches, bark, sap, bulbils, or seeds of plants 1 to 112 (collected or purchased from various locations on Ulleungdo Island) were extracted with 20 times their mass of 70% ethanol in water at room temperature for 3 days, and then filtered under reduced pressure. The filtered extract was concentrated and dried using a rotary evaporator (Buchi, Switzerland) to produce the plant extract. Experimental example 1: Effect of reducing the total amount of melanin

[0090] The whitening effect was confirmed by adding the sample to the culture medium of mouse melanoma cells (B-16 mouse melanoma cells) and measuring the total amount of melanin at the cellular level (Lotan R., Lotan D. Cancer Res. 40:3345-3350, 1980). Before the experiment, toxicity to mouse melanoma cells was evaluated, and a non-toxic concentration was selected for whitening evaluation.

[0091] The samples were treated in the culture medium so that the final concentration was the same as the treatment concentration in the table below, and arbutin, which served as a control, was added to the medium so that it was 200 ppm. Each was then treated with B-16 melanoma cells and cultured for 3 days.

[0092] The cells were then removed from the culture vessel and centrifuged. 1 ml of sodium hydroxide solution (1N concentration) was added to the precipitated cell pellet, which was then heated at 80°C for 10 minutes to dissolve the melanin. The absorbance at 400 nm was measured using a spectrophotometer to determine the amount of melanin produced.

[0093] The amount of melanin per unit cell number (1 × 10 6 The absorbance of each sample was measured by a method showing the absorbance of the sample per cell. The relative total amount of melanin in the experimental group treated with each sample was calculated as an inhibition rate (%) based on the control group without addition. The results are summarized in the table below.

[0094] Experimental Example 2: Promotion of type I collagen synthesis in human fibroblasts

[0095] Each sample was added to the culture medium of human fibroblasts to confirm its effect of promoting type 1 collagen synthesis at the cellular level. Synthesized collagen was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT). To measure the amount of collagen synthesis, each sample was added to the fibroblast culture medium (DMEM medium) and cultured for 48 hours. The culture medium was then sampled and the level of type 1 collagen synthesis at each concentration was measured using a PICP EIA kit at 450 nm using a spectrophotometer.

[0096] To compare the effects, the level of collagen synthesis was measured using the same method for a sample containing untreated fibroblast culture medium (negative control) and a sample containing TGFβ (positive control) at a final concentration of 10 ppb. The increase in collagen synthesis was calculated as the relative collagen synthesis rate compared to the negative control group, and the results are shown in the table below.

[0097] Experimental Example 3. Inhibitory effect of NO production

[0098] To confirm the anti-inflammatory and skin trouble relieving effects of each sample, an experiment was conducted to test the ability to inhibit nitric oxide (NO) formation using the GRIESS method with RAW264.7 cell line (ATCC number: CRL-2278).

[0099] Specifically, mouse macrophage cells, RAW264.7 cells, were subcultured several times, and 3 × 10 cells were placed in each well. 5The cells were placed in a 24-well plate, each containing 100 cells per well, and cultured for 24 hours. The cells were then replaced with cell culture medium diluted to the final concentration shown in the table below. The positive control group was treated with L-NMMA (L-NG-Monomethylarginine), an NO production inhibitor, at a final concentration of 5 ppm and cultured for 30 minutes. Then, the cells were treated with 1 μg / ml of LPS (lipopolysaccharide) as a stimulant and cultured for 24 hours. 100 μl of the supernatant was transferred to a 96-well plate, and 100 μl of GRIESS solution was added. The cells were incubated at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect of each sample. The results are shown in the table below.

[0100] Experimental Example 4. Evaluation of the promotion of hyaluronic acid synthesis

[0101] Human dermal fibroblasts were cultured at a density of 1 × 10 in DMEM medium containing 10% fetal bovine serum. 5 The cells were cultured at 1000 cells / mL and 500 μL of each solution was dispensed into a 24-well plate and cultured for 18 hours. After this, the samples were diluted in DMEM medium without fetal bovine serum and added at the following concentrations. As a positive control, cells were treated with 10 ppb of epidermal growth factor (EGF), which is known to promote hyaluronic acid production. After 24 hours of culture, the cell culture medium was collected and the hyaluronic acid concentration was measured using a Hyaluronan ELISA kit (R&D Systems).

[0102] In addition, the concentration of hyaluronic acid in the control group without addition was taken as the standard (100%), and the concentration of hyaluronic acid in the experimental groups treated with each sample was quantified and shown in the table below.

[0103] Experimental Example 5. Antioxidant effect - Free radical scavenging rate

[0104] Free radical scavenging activity was measured to confirm antioxidant activity. Free radical scavenging activity was measured using DPPH, which was purchased from Sigma Co., Ltd. (USA). A 1.5 mM (0.06 mg / ml) standard DPPH ethanol solution was prepared. Each extract and the antioxidant ascorbic acid (a reference substance) were then added to ethanol to prepare samples at a concentration of 0.1%. These samples were then serially diluted. The samples and the standard DPPH solution were then added in equal proportions, stirred thoroughly, and incubated at 37°C for 30 minutes. The absorbance was measured at 517 nm. A control group was prepared by adding ethanol instead of the samples. Free radical scavenging activity was calculated as the IC50, or half maximal inhibitory concentration, and the results are shown in the table below. IC50 is the concentration of ascorbic acid or the extract required to eliminate 50% of the free radicals in the untreated control group. It is a commonly used method for expressing free radical scavenging activity.

[0105] Experimental Example 6. Antibacterial Effect - Minimum Inhibitory Concentration (MIC) Measurement Method

[0106] The MICs against fermentation cultures of Staphylococcus aureus and Aspergillus niger were measured.

[0107] The MIC of Staphylococcus aureus was measured using Mueller Hinton Broth, the optimal medium, at 10 6 The bacteria were cultured at 37°C for 24 hours at a bacterial concentration of CFU / mL. Aspergillus niger was cultured in potato dextrose broth at 10 5The bacteria were cultured at a bacterial concentration of CFU / mL at 30°C for 48 hours. Serial dilution was used for both bacteria, and after the culture was completed, the turbidity between wells was visually compared to confirm the MIC of the fermented culture.

[0108] The minimum growth inhibitory concentration of each extract is shown in % (w / v), and in each table, MIC (%) means % (w / v) unless otherwise specified.

[0109] Experimental Example 7. Antibacterial effect against bacteria that cause dental caries and periodontitis

[0110] Antibacterial activity tests were conducted to confirm the preventive or therapeutic effects of each sample on caries and periodontitis. To confirm the inhibitory effect on the growth of oral pathogens, antibacterial activity tests were conducted using a paper disc test method with Streptococcus mutans, a typical caries-causing bacterium, and Porphyromonas gingivalis, a typical periodontal disease-causing bacterium.

[0111] After increasing the activity of each oral pathogenic bacterium under the optimal culture conditions shown in the table below, the bacterium was cultured in the optimal medium for each bacterium for about 4 to 6 hours until the turbidity of the culture solution reached Macfarland turbidity No. 0.5 (1.5 x 10 8 ), and 0.1 ml of each oral pathogen was evenly smeared on the plate medium. Thereafter, each sample was inoculated at a concentration of 10 mg / disc and allowed to dry for 1 hour. Next, after culturing for 24 to 48 hours at the optimal temperature for each oral pathogen, the size of the growth inhibition zone (diameter in mm) was measured.

[0112] [Table 1]

[0113] Example 1. Nambu thistle

[0114] [Table 2]

[0115] [Table 3]

[0116] Example 2. Codonopsis

[0117] [Table 4]

[0118] [Table 5]

[0119] Example 3. Goldenrod

[0120] [Table 6]

[0121] [Table 7]

[0122] Example 5. Sesame

[0123] [Table 8]

[0124] [Table 9]

[0125] Example 6. Wild Onion

[0126] [Table 10]

[0127] [Table 11]

[0128] Example 7. Red mountain onion

[0129] [Table 12]

[0130] [Table 13]

[0131] Example 8. Rowan

[0132] [Table 14]

[0133] [Table 15]

[0134] Example 10. Shaku

[0135] [Table 16]

[0136] [Table 17]

[0137] Example 11. Japanese knotweed

[0138] [Table 18]

[0139] [Table 19]

[0140] Example 12. Takeshimaitaya

[0141] [Table 20]

[0142] [Table 21]

[0143] Example 13. Takeshima Fern

[0144] [Table 22]

[0145] [Table 23]

[0146] Example 14. Wasabi

[0147] [Table 24]

[0148] [Table 25]

[0149] Example 15. Triticum vulgare

[0150] [Table 26]

[0151] [Table 27]

[0152] Example 16: Teshima Polygonatum odoratum

[0153] [Table 28]

[0154] [Table 29]

[0155] Example 17. Kizuta

[0156] [Table 30]

[0157] [Table 31]

[0158] Example 18. Violet

[0159] [Table 32]

[0160] [Table 33]

[0161] Example 19. Takeshima Campanula

[0162] [Table 34]

[0163] [Table 35]

[0164] Example 20. Takeshima lily

[0165] [Table 36]

[0166] [Table 37]

[0167] Example 21. Takeshima stag beetle

[0168] [Table 38]

[0169] [Table 39]

[0170] Example 22. Taigome

[0171] [Table 40]

[0172] [Table 41]

[0173] Example 23. Chickweed

[0174] [Table 42]

[0175] [Table 43]

[0176] Example 24. Houttuynia cordata

[0177] [Table 44]

[0178] [Table 45]

[0179] Example 25. Rusticaria

[0180] [Table 46]

[0181] [Table 47]

[0182] Example 26. Yamakitsune nobotan

[0183] [Table 48]

[0184] [Table 49]

[0185] Example 27. Akaso

[0186] [Table 50]

[0187] [Table 51]

[0188] Example 28. Dianthus sieboldii

[0189] [Table 52]

[0190] [Table 53]

[0191] Example 29. Rumex crispus

[0192] [Table 54]

[0193] [Table 55]

[0194] Example 30. Hypericum

[0195] [Table 56]

[0196] [Table 57]

[0197] Example 31. Jiaogulan

[0198] [Table 58]

[0199] [Table 59]

[0200] Example 32. Melon

[0201] [Table 60]

[0202] [Table 61]

[0203] Example 33: Takeshima Hatazao

[0204] [Table 62]

[0205] [Table 63]

[0206] Example 34. Persimmon

[0207] [Table 64]

[0208] [Table 65]

[0209] Example 36. Umegasasou

[0210] [Table 66]

[0211] [Table 67]

[0212] Example 37. Ardisia crassipes

[0213] [Table 68]

[0214] [Table 69]

[0215] Example 38. Hamabossu

[0216] [Table 70]

[0217] [Table 71]

[0218] Example 39. Climbing hydrangea

[0219] [Table 72]

[0220] [Table 73]

[0221] Example 40. Sedum crenata

[0222] [Table 74]

[0223] [Table 75]

[0224] Example 41. Takeshima kirinsou

[0225] [Table 76]

[0226] [Table 77]

[0227] Example 42. Zdayakush

[0228] [Table 78]

[0229] [Table 79]

[0230] Example 43: Kawara Saiko

[0231] [Table 80]

[0232] [Table 81]

[0233] Example 44. Nettle

[0234] [Table 82]

[0235] [Table 83]

[0236] Example 45. Scutellaria

[0237] [Table 84]

[0238] [Table 85]

[0239] Example 46. Medicago sativa

[0240] [Table 86]

[0241] [Table 87]

[0242] Example 47. Noharakusa Fuji

[0243] [Table 88]

[0244] [Table 89]

[0245] Example 48. Purple loosestrife

[0246] [Table 90]

[0247] [Table 91]

[0248] Example 49. Water hyacinth

[0249] [Table 92]

[0250] [Table 93]

[0251] Example 50. Red flower

[0252] [Table 94]

[0253] [Table 95]

[0254] Example 51. Evening primrose

[0255] [Table 96]

[0256] [Table 97]

[0257] Example 52. Cranberry

[0258] [Table 98]

[0259] [Table 99]

[0260] Example 53. Diamondsou

[0261] [Table 100]

[0262] [Table 101]

[0263] Example 54. Lamium sieboldii

[0264] [Table 102]

[0265] [Table 103]

[0266] Example 55. Shimatemari Spirea

[0267] [Table 104]

[0268] [Table 105]

[0269] Example 56: Takeshima Corydalis

[0270] [Table 106]

[0271] [Table 107]

[0272] Example 57. Rumberry

[0273] [Table 108]

[0274] [Table 109]

[0275] Example 58. Takeshima Shishiudo

[0276] [Table 110]

[0277] [Table 111]

[0278] Example 59. Takeshima Violet

[0279] [Table 112]

[0280] [Table 113]

[0281] Example 60. Masaki

[0282] [Table 114]

[0283] [Table 115]

[0284] Example 61. Wild grapes

[0285] [Table 116]

[0286] [Table 117]

[0287] Example 62. Udo

[0288] [Table 118]

[0289] [Table 119]

[0290] Example 63. Aralia

[0291] [Table 120]

[0292] [Table 121]

[0293] Example 64. Fatsia

[0294] [Table 122]

[0295] [Table 123]

[0296] Example 65. Button vine

[0297] [Table 124]

[0298] [Table 125]

[0299] Example 66. Brown-eared fungus

[0300] [Table 126]

[0301] [Table 127]

[0302] Example 67. Morning glory

[0303] [Table 128]

[0304] [Table 129]

[0305] Example 68. Sea Bindweed

[0306] [Table 130]

[0307] [Table 131]

[0308] Example 69. Barberry

[0309] [Table 132]

[0310] [Table 133]

[0311] Example 70. Kawamidori

[0312] [Table 134]

[0313] [Table 135]

[0314] Example 71. Carbana

[0315] [Table 136]

[0316] [Table 137]

[0317] Example 72. Leptospermum hirsutum

[0318] [Table 138]

[0319] [Table 139]

[0320] Example 73. Mehajiki

[0321] [Table 140]

[0322] [Table 141]

[0323] Example 74. Giant larch

[0324] [Table 142]

[0325] [Table 143]

[0326] Example 75. Viburnum maximowicziana

[0327] [Table 144]

[0328] [Table 145]

[0329] Example 76. Celandine

[0330] [Table 146]

[0331] [Table 147]

[0332] Example 77. Daffodil

[0333] [Table 148]

[0334] [Table 149]

[0335] Example 78. Japanese silverleaf

[0336] [Table 150]

[0337] [Table 151]

[0338] Example 79. Inula gracilis

[0339] [Table 152]

[0340] [Table 153]

[0341] Example 80. Butterbur

[0342] [Table 154]

[0343] [Table 155]

[0344] Example 81. Takeshima Shojosuge

[0345] [Table 156]

[0346] [Table 157]

[0347] Example 82. Blue sedge

[0348] [Table 158]

[0349] [Table 159]

[0350] Example 83. Purple grass

[0351] [Table 160]

[0352] [Table 161]

[0353] Example 84. Chikarashiba

[0354] [Table 162]

[0355] [Table 163]

[0356] Example 85. Yatake

[0357] [Table 164]

[0358] [Table 165]

[0359] Example 86. Kurile Sasa

[0360] [Table 166]

[0361] [Table 167]

[0362] Example 87. Clara

[0363] [Table 168]

[0364] [Table 169]

[0365] Example 88. Daylily

[0366] [Table 170]

[0367] [Table 171]

[0368] Example 89. Tiger lily

[0369] [Table 172]

[0370] [Table 173]

[0371] Example 90. Liriope

[0372] [Table 174]

[0373] [Table 175]

[0374] Example 91. Rhubarb

[0375] [Table 176]

[0376] [Table 177]

[0377] Example 92. Cedar

[0378] [Table 178]

[0379] [Table 179]

[0380] Example 93. Machilus thunbergii

[0381] [Table 180]

[0382] [Table 181]

[0383] Example 94. Pinus pentaphylla

[0384] [Table 182]

[0385] [Table 183]

[0386] Example 95. Tsuga

[0387] [Table 184]

[0388] [Table 185]

[0389] Example 96. White Poplar

[0390] [Table 186]

[0391] [Table 187]

[0392] Example 97. Yuzureha

[0393] [Table 188]

[0394] [Table 189]

[0395] Example 98. Zelkova

[0396] [Table 190]

[0397] [Table 191]

[0398] Example 99. Siberian hackberry

[0399] [Table 192]

[0400] [Table 193]

[0401] Example 100. Halibut

[0402] [Table 194]

[0403] [Table 195]

[0404] Example 101. Alder

[0405] [Table 196]

[0406] [Table 197]

[0407] Example 102. Takeshima Linden

[0408] [Table 198]

[0409] [Table 199]

[0410] Example 103. White jasmine

[0411] [Table 200]

[0412] [Table 201]

[0413] Example 104: Takeshima cherry

[0414] [Table 202]

[0415] [Table 203]

[0416] Example 105. Aoki

[0417] [Table 204]

[0418] [Table 205]

[0419] Example 106. Okinoha

[0420] [Table 206]

[0421] [Table 207]

[0422] Example 107. Shimakihada

[0423] [Table 208]

[0424] [Table 209]

[0425] Example 108. Zanthoxylum chinense

[0426] [Table 210]

[0427] [Table 211]

[0428] Example 109. Ligustrum japonicum

[0429] [Table 212]

[0430] [Table 213]

[0431] Example 110. Goldenrod

[0432] [Table 214]

[0433] [Table 215]

[0434] Example 111. Silk tree

[0435] [Table 216]

[0436] [Table 217]

[0437] Example 112. Takasaburo

[0438] [Table 218]

[0439] [Table 219]

[0440] Formulation Example 1: Preparation of pharmaceutical formulations

[0441] 1. Powder manufacturing

[0442] Plant extract 0.001g 1g lactose

[0443] The above ingredients were mixed and filled into an airtight bag to prepare a powder.

[0444] 2. Tablet manufacturing

[0445] Plant extract 0.2mg 100mg corn starch Lactose 100mg Magnesium stearate 2mg

[0446] The above ingredients were mixed and then compressed into tablets by a conventional tablet manufacturing method.

[0447] 3. Capsule manufacturing

[0448] Plant extract 0.2mg 100mg corn starch Lactose 100mg Magnesium stearate 2mg

[0449] The above ingredients were mixed and then filled into gelatin capsules by a conventional capsule manufacturing method to prepare capsules.

[0450] 4. Manufacturing of pills

[0451] Plant extract 0.003g Lactose 1.5g 1g glycerin 0.5g xylitol

[0452] The above ingredients were mixed and then prepared in a conventional manner so that each pill weighed 4 g.

[0453] 5. Granule production

[0454] 2mg plant extract Soybean extract 50mg 200mg glucose Starch 600mg

[0455] After mixing the above ingredients, 100 mg of 30% ethanol was added and dried at 60°C to form granules, which were then filled into a sachet.

[0456] Formulation Example 2: Manufacturing of cosmetics

[0457] 1. Manufacturing of softening lotion (skin lotion)

[0458] A softening lotion containing a plant extract as an active ingredient according to the following composition was prepared by a conventional method.

[0459] Plant extract 0.1% by weight β-1,3-glucan 1.0% by weight Butylene glycol 2.0% by weight Propylene glycol 2.0% by weight Carboxyvinyl polymer 0.1% by weight PEG-12 nonylphenyl ether 0.2% by weight Polysorbate 80 0.4% by weight Ethanol 10.0% by weight Triethanolamine 0.1% by weight Preservative 0.05% by weight Pigment 0.05% by weight Fragrance 0.05% by weight Purified water to 100% by weight

[0460] 2. Manufacturing of nourishing lotion (milk)

[0461] A nourishing lotion containing a plant extract as an active ingredient according to the following composition was prepared by a conventional method.

[0462] Plant extract 0.1% by weight β-1,3-glucan 1.0% by weight Beeswax 4.0% by weight Polysorbate 60 1.5% by weight Sorbitan sesquioleate 1.5% by weight Liquid paraffin 0.5% by weight Caprylic / capric triglyceride 5.0% by weight Glycerin 3.0% by weight Butylene glycol 3.0% by weight Propylene glycol 3.0% by weight Carboxyvinyl polymer 0.1% by weight Triethanolamine 0.2% by weight Preservative 0.05% by weight Pigment 0.05% by weight Fragrance 0.05% by weight Purified water to 100% by weight

[0463] 3. Manufacturing of nutritional cream

[0464] A nourishing cream containing a plant extract as an active ingredient according to the following composition was prepared by a conventional method.

[0465] Plant extract 0.2% by weight β-1,3-glucan 5.0% by weight Beeswax 10.0% by weight Polysorbate 60 1.5% by weight PEG60 hydrogenated castor oil 2.0% by weight Sorbitan sesquioleate 0.5% by weight Liquid paraffin 10.0% by weight Squalane 5.0% by weight Caprylic / capric triglyceride 5.0% by weight Glycerin 5.0% by weight Butylene glycol 3.0% by weight Propylene glycol 3.0% by weight Triethanolamine 0.2% by weight Preservative 0.05% by weight Pigment 0.05% by weight Fragrance 0.05% by weight Purified water to 100% by weight

[0466] 4. Manufacturing massage cream

[0467] A massage cream containing a plant extract as an active ingredient according to the following composition was prepared by a conventional method.

[0468] Plant extract 0.1% by weight β-1,3-glucan 3.0% by weight Beeswax 10.0% by weight Polysorbate 60 1.5% by weight PEG60 hydrogenated castor oil 2.0% by weight Sorbitan sesquioleate 0.8% by weight Liquid paraffin 40.0% by weight Squalane 5.0% by weight Caprylic / capric triglyceride 4.0% by weight Glycerin 5.0% by weight Butylene glycol 3.0% by weight Propylene glycol 3.0% by weight Triethanolamine 0.2% by weight Preservative 0.05% by weight Pigment 0.05% by weight Fragrance 0.05% by weight Purified water to 100% by weight

[0469] 5. Pack manufacturing

[0470] A pack containing the plant extract as an active ingredient according to the composition below was prepared by a conventional method.

[0471] Plant extract 0.2% by weight β-1,3-glucan 1.0% by weight Polyvinyl alcohol 13.0% by weight Sodium carboxymethylcellulose 0.2% by weight Glycerin 5.0% by weight Allantoin 0.1% by weight Ethanol 6.0% by weight PEG-12 nonylphenyl ether 0.3% by weight Polysorbate 60 0.3% by weight Preservative 0.05% by weight Pigment 0.05% by weight Fragrance 0.05% by weight Purified water to 100% by weight

[0472] Formulation Example 3: Preparation of topical skin preparation

[0473] 1. Gel production

[0474] A gel containing a plant extract as an active ingredient was prepared by a conventional method according to the composition below.

[0475] Plant extract 0.1% by weight β-1,3-glucan 0.1% by weight Sodium ethylenediamine acetate 0.05% by weight Glycerin 5.0% by weight Carboxyvinyl polymer 0.3% by weight Ethanol 5.0% by weight PEG-60 hydrogenated castor oil 0.5% by weight Triethanolamine 0.3% by weight Preservative 0.05% by weight Pigment 0.05% by weight Fragrance 0.05% by weight Purified water to 100% by weight

[0476] 2. Ointment manufacturing

[0477] An ointment containing the plant extract as an active ingredient according to the composition below was prepared by a conventional method.

[0478] Plant extract 0.5% by weight β-1,3-glucan 10.0% by weight Beeswax 10.0% by weight Polysorbate 60 5.0% by weight PEG60 hydrogenated castor oil 2.0% by weight Sorbitan sesquioleate 0.5% by weight Vaseline 5.0% by weight Liquid paraffin 10.0% by weight Squalane 5.0% by weight Shea butter 3.0% by weight Caprylic / capric triglyceride 5.0% by weight Glycerin 10.0% by weight Propylene glycol 10.2% by weight Triethanolamine 0.2% by weight Preservative 0.05% by weight Pigment 0.05% by weight Fragrance 0.05% by weight Purified water to 100% by weight

[0479] 3. Manufacture of topical medications (gel ointments)

[0480] A gel ointment containing the plant extract as an active ingredient according to the composition below was prepared by a conventional method.

[0481] Plant extract 0.5% by weight β-1,3-glucan 10.0% by weight Polyacrylic acid (Carbopol 940) 1.5% by weight Isopropanol 5.0% by weight Hexylene glycol 25.0% by weight Triethanolamine 1.7% by weight Deionized water to 100% by weight

[0482] 4. Manufacturing of topical medications (patches)

[0483] A patch containing the plant extract as an active ingredient according to the composition below was prepared by a conventional method.

[0484] Plant extract 0.5% by weight β-1,3-glucan 3.0% by weight Hexylene glycol 20.0% by weight Diethylamine 0.7% by weight Polyacrylic acid (Carbopol 934P) 1.0% by weight Sodium sulfite 0.1% by weight Polyoxyethylene lauryl ether (EO=9) 1.0% by weight Polyhydroxyethylene cetyl stearyl ether (Cetomacrogol 1000) 1.0% by weight 2.5% by weight of viscous paraffin oil 1656632050951_0 Ester / capric acid ester (Cetiol LC) 2.5% by weight Polyethylene glycol 400 3.0% by weight Deionized water to 100% by weight

[0485] Formulation Example 4: Food manufacturing

[0486] A food product containing the plant extract of the present invention was prepared as follows.

[0487] 1. Flour food manufacturing

[0488] 0.05 to 1.0 parts by weight of the plant extract was added to wheat flour, and the mixture was used to produce bread, cakes, cookies, crackers and noodles to produce health foods.

[0489] 2. Dairy product manufacturing

[0490] 0.2 parts by weight of the plant extract was added to milk, and the milk was used to produce various dairy products such as butter and ice cream.

[0491] 3. Zen food production

[0492] Brown rice, barley, glutinous rice, and adlay were gelatinized and dried using a known method, then roasted and ground into powder with a particle size of 60 mesh using a grinder. Black beans, black sesame seeds, and perilla seeds were also steamed and dried using a known method, then roasted and ground into powder with a particle size of 60 mesh using a grinder. The plant extracts were concentrated under reduced pressure using a vacuum concentrator, sprayed, and dried in a hot air dryer. The dried product was then ground to a particle size of 60 mesh using a grinder to obtain dry powder.

[0493] The dry powders of the grains, nuts and plant extracts prepared above were mixed in the following proportions with 100 parts by weight of the mixed powder.

[0494] Grains (30 parts by weight of brown rice, 15 parts by weight of barley, 20 parts by weight of barley), Nuts and seeds (perilla 7 parts by weight, black beans 8 parts by weight, black sesame 7 parts by weight), Plant extract (0.1 part by weight), Reishi mushroom (0.5 parts by weight), Dio (0.5 parts by weight)

[0495] Formulation Example 5: Beverage Production

[0496] 1. Healthy drink manufacturing

[0497] Plant extract 0.1mg Citric acid 1000mg 100g oligosaccharides Plum concentrate 2g 1g taurine Add purified water to make a total of 900mL

[0498] The above ingredients were mixed according to a conventional method for producing a health drink, and then stirred and heated at 85°C for about 1 hour. The resulting solution was filtered, placed in a sterilized 2L container, sealed, sterilized, and stored in a refrigerator before being used to produce the health drink composition of the present invention.

[0499] The above composition ratio is a preferred example of a mixture of ingredients that are relatively suitable for beverages, but the composition ratio may be arbitrarily modified depending on the demand group, demand country, intended use, and regional and ethnic preferences.

[0500] 2. Vegetable juice production

[0501] Health-promoting vegetable juice was produced by adding 1 g of the plant extract of the present invention to 1,000 mL of tomato or carrot juice.

[0502] 3. Fruit juice production

[0503] Health-promoting fruit juices were produced by adding 1 g of plant extract to 1,000 mL of apple or grape juice. [Industrial Applicability]

[0504] The composition containing the plant extract of the present invention has a skin whitening effect by reducing the total amount of melanin and the activity of tyrosinase in skin melanocytes, promotes collagen synthesis in skin fibroblasts and inhibits collagenase activity, thereby promoting skin regeneration and improving skin elasticity or wrinkles, inhibits NO production and has an anti-inflammatory or skin soothing effect, promotes the production of hyaluronic acid in fibroblasts, thereby increasing the moisture content of the skin and having a moisturizing effect, scavenges free radicals and has an antioxidant effect, and also exhibits a wide range of antibacterial effects against various bacteria, making it suitable for use as a cosmetic composition, pharmaceutical composition, topical skin preparation, or food composition.

Claims

1. A cosmetic composition for moisturizing skin, comprising a plant extract of geometries (scientific name: Anthriscus sylvestris, English name: Wild chervil) as an active ingredient.

2. The cosmetic composition according to claim 1 , wherein the plant extract is a substance extracted from a plant using water, an organic solvent, or a mixture thereof.

3. 3. The cosmetic composition according to claim 2, wherein the organic solvent is a lower alcohol having 1 to 6 carbon atoms, a polyhydric alcohol, a hydrocarbon solvent, or a mixture thereof.

4. 2. The cosmetic composition according to claim 1, wherein the plant extract is contained in an amount of 0.0001 to 10 parts by weight per 100 parts by weight of the composition.

5. A pharmaceutical composition for moisturizing the skin, comprising a plant extract of Wild chervil (scientific name: Anthriscus sylvestris, English name: Wild chervil) as an active ingredient.

6. A moisturizing topical skin preparation containing a plant extract of wild chervil (scientific name: Anthriscus sylvestris, English name: Wild chervil) as an active ingredient.

7. A food composition for moisturizing the skin, comprising a plant extract of wild chervil (scientific name: Anthriscus sylvestris, English name: Wild chervil) as an active ingredient.

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