Method for producing plant-derived extract composition

Abstract

In one aspect, a method for producing a plant-derived extract composition that has excellent storage stability and can reduce discoloration that occurs over time is provided. [Solution] In one aspect, the present disclosure relates to a method for producing a plant-derived extract composition having excellent storage stability, the method including a step of obtaining an extract composition from hydroponically grown rosemary.

Description

[Technical Field]

[0001] The present disclosure relates to a method for producing a plant-derived extract composition and a plant-derived extract composition. [Background technology]

[0002] To improve the skin's moisture retention ability, prevent and improve rough skin, and prevent and improve skin aging such as the formation of wrinkles and the reduction of texture, it is believed that it is effective to act on the epidermal cells of the skin, promote the keratinization of skin cells, encourage the formation of a healthy stratum corneum, and improve the stratum corneum barrier function to protect the body from external stimuli and other factors.

[0003] It is said that maintaining the stratum corneum's moisture content and flexibility is important for the health of this barrier function. Aging, dryness, ultraviolet rays, and other factors can disrupt the cell turnover process, leading to abnormalities in the formation of stratum corneum cells and the structure of intercellular lipids. This is known to lead to various skin disorders and skin problems, such as rough skin.

[0004] Here, turnover refers to the continuous repetition of keratinocyte (epidermal keratinocyte) proliferation in the basal layer, the cornification process, and the peeling of the stratum corneum. These keratinocytes form each layer, from basal membrane cells, spinous cells, granular cells, and corneocytes, cornifying sequentially outward. Proteins that make up the cornified envelope (CE) are synthesized from the upper spinous layer to the granular layer. Furthermore, as they reach the stratum corneum, transglutaminase (TGM-1) binds matrix proteins such as involucrin (IVL), loricrin (LOR), and cystatin to the keratinocyte cell membrane, forming insolubilized CE. Furthermore, ceramides and other proteins covalently bind to the insolubilized CE, forming the basis of the stratum corneum's barrier function.

[0005] Conventionally, skin problems such as rough skin caused by a decrease in the stratum corneum barrier function have been resolved by supplementing the stratum corneum barrier function with creams containing ceramides, etc. However, the improvement in CE of stratum corneum cells is insufficient, and there is a demand for ingredients (agents, compositions, etc.) that can promote the formation of healthy CE. For example, Patent Document 1 proposes a composition for improving skin viscoelasticity, which contains an inhibitor of an increase in extracellular ATP concentration, in which the weight ratio of (A) rosmarinic acid to (B) luteolin glucuronide is (A):(B) = 1:0.2 to 1.5. Patent Document 2 proposes a filaggrin production promoter containing rosmarinic acid and / or eriodictyol 7-O-rutinoside as active ingredients to promote the mRNA expression of profilaggrin, a precursor of filaggrin that is involved in improving the skin's moisturizing function. Patent Document 3 proposes an involucrin expression promoter and a stratum corneum formation promoter that contain one or more plants or extracts thereof selected from Japanese pepper, peppermint, eucalyptus, saxifrage, rosemary, asparagus, comfrey, shiitake mushroom, calamus, thyme, burnet, gambir, ginkgo, kudzu, Chinese quince, gardenia, loquat, cinchona, hawthorn, clove, mugwort, and Phellodendron amurense as agents capable of maintaining healthy skin barrier function. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] International Publication No. 2020 / 158912 [Patent Document 2] Japanese Patent Application Laid-Open No. 2010-90037 [Patent Document 3] Japanese Patent Application Laid-Open No. 2007-277149 Summary of the Invention [Problem to be solved by the invention]

[0007] Rosmarinic acid has attracted attention as a component for improving (promoting) keratinization. Rosmarinic acid is a type of polyphenol found in large amounts in plants of the Lamiaceae family, such as rosemary. A known plant-derived extract composition containing rosmarinic acid is, for example, rosemary extract extracted from rosemary (Japanese name: Mannenrou). Plant-derived extract compositions such as rosemary extract generally tend to discolor over time, which can damage the image of topical products, foods, and the like that use the plant-derived extract composition. The discoloration is thought to be caused by the discoloration of the plant-derived extract composition itself. Therefore, there is a demand for a plant-derived extract composition that has excellent storage stability and can reduce discoloration that occurs over time.

[0008] Therefore, in one aspect, the present disclosure provides a method for producing a plant-derived extract composition that has excellent storage stability and is capable of reducing discoloration that occurs over time. [Means for solving the problem]

[0009] In one aspect, the present disclosure relates to a method for producing a plant-derived extract composition, the method comprising obtaining the extract composition from hydroponically grown rosemary.

[0010] In one aspect, the present disclosure relates to a plant-derived extract composition containing rosmarinic acid, wherein the mass ratio of sugars to rosmarinic acid (sugars / rosmarinic acid) is 10 or less.

[0011] In one aspect, the present disclosure relates to a cosmetic product containing the extract composition of the present disclosure. [Effects of the Invention]

[0012] According to one aspect of the present disclosure, a method for producing a plant-derived extract composition that has excellent storage stability and can reduce discoloration that occurs over time can be provided. DETAILED DESCRIPTION OF THE INVENTION

[0013] Generally, plant-derived extract compositions are mixtures of various components and contain many unexpected impurities, so the coloring components of plant-derived extract compositions have not been generally known. The present inventors have found that the concentration of sugars contained in hydroponically grown rosemary is reduced compared to that in open-field grown rosemary, and further found that the storage stability of an extract composition extracted from hydroponically grown rosemary is improved and that discoloration that occurs over time can be reduced.

[0014] That is, in one aspect, the present disclosure relates to a method for producing a plant-derived extract composition (hereinafter also referred to as the "method for producing the extract composition of the present disclosure"), which includes a step of obtaining an extract composition from hydroponically grown rosemary (hereinafter also referred to as the "extraction step").

[0015] According to one aspect of the present disclosure, a method for producing a plant-derived extract composition that has excellent storage stability and can reduce discoloration that occurs over time can be provided.

[0016] Although the details of the mechanism by which the effects of the present disclosure are manifested are not clear, it is presumed as follows. In the present disclosure, by obtaining an extract composition from hydroponically grown rosemary, it is believed that the mass ratio of sugars to rosmarinic acid (sugars / rosmarinic acid) in the plant-derived extract composition can be reduced to a predetermined value or less, thereby reducing the sugar concentration in the extract composition. In hydroponic cultivation, the roots are more efficiently absorbed of nutrient solution and oxygen, resulting in a faster growth rate than open-field cultivation. Furthermore, not only nutrient solution but also sugar is essential for plant growth. In other words, in hydroponic cultivation, where plants grow faster, more sugar is consumed within the plant body, resulting in a reduced sugar content. Therefore, it is believed that the extract composition obtained from hydroponically grown rosemary can suppress discoloration and improve storage stability. However, the present disclosure need not be construed as being limited to these mechanisms.

[0017] In one or more embodiments, the plant of the present disclosure may be a plant containing rosmarinic acid. The plant containing rosmarinic acid is not particularly limited as long as it contains rosmarinic acid, and examples thereof include Lamiaceae plants. Examples of Lamiaceae plants include herb plants of the Lamiaceae family, such as rosemary (Rosmarinus officinalis), perilla, lemon balm, and common sage. The plant part used for extraction may be the whole or a part of the plant, and can be appropriately selected depending on the purpose. Suitable plant parts for extraction include leaves, stems, flowers, fruits, pericarp, pits, above-ground parts, and mixtures thereof. Among these, leaves are preferred from the viewpoint of efficient extraction of rosmarinic acid.

[0018] [Extraction process] In one or more embodiments, the method for producing an extract composition of the present disclosure includes a step of obtaining an extract composition from hydroponically grown rosemary (extraction step). Examples of extraction methods used in the extraction step include soaking, decoction, percolation, reflux extraction, supercritical fluid extraction, ultrasonic extraction, and microwave extraction. A preferred method involves soaking a plant in an extraction solvent. For example, a method for extracting rosmarinic acid involves placing a certain amount of rosemary in a kettle, adding a soaking solvent (e.g., water), and then soaking the mixture at 25°C for 24 hours. Stirring may or may not be performed during soaking. Therefore, in one or more embodiments, the extraction step is a step of soaking hydroponically grown rosemary in an extraction solvent to obtain an extract composition.

[0019] <Plants used for extraction> The plant used in the extraction process is hydroponically grown rosemary. The method of cultivating rosemary is hydroponics, a form of nutrient solution cultivation. Here, hydroponics is a type of nutrient solution cultivation that does not require a solid cultivation medium, in other words, a cultivation method in which plants are grown by providing them with a nutrient solution without using soil or a solid medium. The hydroponic cultivation method is not particularly limited, and in one or more embodiments, it is any one of thin film hydroponic cultivation, flooded hydroponic cultivation, and spray hydroponic cultivation, or a combination thereof. Here, Nutrient Film Technique (NFT) is a cultivation method in which plants are cultivated by allowing a nutrient solution (culture solution) to flow little by little down a gently sloping cultivation bed. Deep Flow Technique (DFT) is a cultivation method in which a large amount of nutrient solution is stored in a horizontal cultivation bed and plants are cultivated while the nutrient solution is circulated. Spray hydroponic cultivation is a cultivation method in which plants are cultivated by spraying the nutrient solution in the form of a mist onto the roots of the plants.

[0020] (nutrient solution) The nutrient solution used in the hydroponic cultivation may be any liquid containing nutrients and water for growing rosemary. Examples of nutrient solutions include liquids containing nitrogen, phosphoric acid, potassium, etc. Commercially available nutrient solutions include Hi-Tempo Ar Solution (manufactured by Mitsubishi Chemical Aqua Solutions Co., Ltd.) and Hi-Tempo Cu Solution (manufactured by Mitsubishi Chemical Aqua Solutions Co., Ltd.). The temperature of the nutrient solution used in the hydroponic cultivation step does not particularly need to be controlled, but may be, for example, the same temperature as the cultivation temperature (set temperature of the equipment) described below. The pH of the nutrient solution used in the hydroponic cultivation is preferably 7.5 or less from the viewpoint of increasing the yield. The pH is the pH of the nutrient solution during cultivation, and can be measured using a pH / EC meter, the value being the value 2 minutes after immersing the electrodes of the pH / EC meter in the nutrient solution. In one or more embodiments, the pH of the nutrient solution used in the hydroponic cultivation is preferably adjusted at least once a day to be within the above-mentioned pH range in order to increase the yield. Therefore, it is preferable that the pH of the nutrient solution used in the hydroponic cultivation is maintained at 7.5 or less. The pH of the nutrient solution can be adjusted using, for example, at least one pH adjuster selected from ammonia, sulfuric acid, phosphoric acid, and nitric acid. The EC (electrical conductivity) of the nutrient solution used in the hydroponic cultivation can be, for example, 0.5 mS / cm or more and 2.0 mS / cm or less, from the viewpoint of increasing the yield. The EC is the EC of the nutrient solution during cultivation and can be measured by immersing the electrodes of a pH / EC meter in the nutrient solution. Here, the EC of the nutrient solution is a value indicating the fertilizer concentration. In one or more embodiments, the EC of the nutrient solution used in the hydroponic cultivation step is preferably adjusted at least once a day to fall within the above-mentioned EC range, from the viewpoint of increasing the yield. Therefore, it is preferable that the electrical conductivity (EC) of the nutrient solution used in the hydroponic cultivation be maintained in the range of 0.5 mS / cm to 2.0 mS / cm. For example, tap water can be used to adjust the EC of the nutrient solution.

[0021] (Cultivation temperature) From the same viewpoint, the average cultivation temperature for one day (24 hours) during the hydroponic cultivation period is 10°C or higher and 35°C or lower. The average cultivation temperature during the daytime (light period) during the hydroponic cultivation period is, for example, 5°C or higher and 38°C or lower, from the viewpoint of promoting plant growth. From the same viewpoint, the average cultivation temperature during the night (dark period) during the hydroponic cultivation period is, for example, 5°C or higher and 30°C or lower. In this disclosure, "daytime" refers to sunrise to sunset, and "nighttime" refers to sunset to sunrise.

[0022] <Extraction solvent> The extraction solvent used in the extraction step may be either a polar solvent or a non-polar solvent. Examples of the extraction solvent include water, monohydric alcohols such as methanol, ethanol, propanol, and butanol, and polyhydric alcohols such as 1,3-propanediol, dipropylene glycol, pentanediol, hexanediol, propylene glycol, butylene glycol, and glycerin, and mixtures thereof. Among these, at least one selected from ethanol, glycerin, 1,3-propanediol, butylene glycol, and aqueous solutions thereof is preferred from the viewpoint of efficient extraction of rosmarinic acid. In one or more embodiments, the extraction solvent is preferably an aqueous butylene glycol solution from the viewpoint of efficiently extracting rosmarinic acid. When an aqueous solution of alcohol (monohydric alcohol and / or polyhydric alcohol) is used as the extraction solvent, in one or more embodiments, the concentration thereof is preferably more than 0% by mass and not more than 20% by mass, or more than 0% by volume and not more than 10% by volume, from the viewpoint of efficiently extracting rosmarinic acid. On the other hand, the concentration of the aqueous solution of alcohol (monohydric alcohol and / or polyhydric alcohol) is preferably 30% by volume or more and not more than 90% by volume, or 40% by volume or more and not more than 60% by volume, from the viewpoint of efficiently extracting highly hydrophobic components.

[0023] In one or more embodiments, the extraction solvent used in the extraction step may further contain other components as needed, such as a preservative to prevent decay and a spreading agent to promote penetration into the plant body.

[0024] From the viewpoint of efficient extraction of rosmarinic acid, the amount of the extraction solvent used in the extraction step is preferably 3 times or more, more preferably 5 times or more, and even more preferably 10 times or more, relative to the weight or dry weight of the plant, and from the same viewpoint, it is preferably 100 times or less, more preferably 75 times or less, and even more preferably 50 times or less. From the same viewpoint, the amount of the extraction solvent used in the extraction step is preferably 3 to 100 times, more preferably 5 to 75 times, and even more preferably 10 to 50 times, relative to the weight or dry weight of the plant.

[0025] <Extraction temperature> The temperature of the extraction solvent used for extraction (extraction temperature) or the temperature at which the plant is immersed in the extraction solvent (immersion temperature) is preferably 5°C or higher, more preferably 15°C or higher, and even more preferably 20°C or higher, from the viewpoint of efficient extraction of rosmarinic acid. From the same viewpoint, the extraction temperature or the immersion temperature is preferably 5°C or higher and 35°C or lower, more preferably 15°C or higher and 30°C or lower, and even more preferably 20°C or higher and 30°C or lower.

[0026] <Extraction time> The extraction time or the time for soaking the plant in the extraction solvent (soaking time) is preferably 5 hours or more, more preferably 12 hours or more, and even more preferably 24 hours or more, from the viewpoint of efficient extraction of rosmarinic acid, and from the same viewpoint, it is preferably 120 hours or less, more preferably 72 hours or less, and even more preferably 36 hours or less. From the same viewpoint, the extraction time or the soaking time is preferably 5 hours or more and 120 hours or less, more preferably 12 hours or more and 72 hours or less, and even more preferably 24 hours or more and 36 hours or less.

[0027] In one or more embodiments, the extract composition of the present disclosure can be obtained by filtering using a filter. Thus, in one or more embodiments, the extraction step is a step of immersing hydroponically grown rosemary in an extraction solvent and then filtering using a filter to obtain an extract composition. Examples of filters used for filtration include filter paper, membrane filters, cartridge filters, and disposable filters.

[0028] <Pre-extraction treatment> In one or more embodiments, the plant before extraction or the plant before immersion in the extraction solvent may be blanched. Therefore, in one or more embodiments, the extraction step may include a step of blanching hydroponically grown rosemary before extraction. In one or more embodiments, the blanching is preferably performed within three days after harvesting, more preferably within one day after harvesting, and even more preferably on the day of harvesting. A common method can be used for the blanching treatment. For example, a plant such as rosemary can be subjected to one or more of the following blanching treatments: boiling, microwave treatment, and steam treatment. The blanching temperature can be, for example, 100 to 150°C, and the blanching time can be, for example, 5 to 60 minutes.

[0029] <Drying process> In one or more embodiments, the plant before extraction or the plant before soaking in the extraction solvent may be dried after pre-extraction treatment. Therefore, in one or more embodiments, the extraction step may include a step of blanching hydroponically grown rosemary and then drying it before extraction. The drying method may be a common method, such as natural drying, hot air drying, freeze drying, reduced pressure drying, or spray drying. The hot air drying temperature may be, for example, 30°C to 100°C, and the drying time may be, for example, 1 to 24 hours.

[0030] The extract composition produced by the method for producing an extract composition of the present disclosure may be further purified to produce a purified product. Therefore, the method for producing an extract composition of the present disclosure may further include a step of purifying the extract composition.

[0031] [Extract composition] In one aspect, the present disclosure relates to a plant-derived extract composition (hereinafter also referred to as the "extract composition of the present disclosure") containing rosmarinic acid and having a mass ratio of sugars to rosmarinic acid (sugars / rosmarinic acid) of 10 or less. Examples of the plant include those mentioned above, with rosemary being preferred. In one or more embodiments, the plant is preferably hydroponically grown. In one or more embodiments, the extract composition of the present disclosure is an extract composition produced by the method for producing an extract composition of the present disclosure. According to one or more embodiments of the present disclosure, an extraction composition having excellent storage stability can be provided.

[0032] (rosmarinic acid) In one or more embodiments, the extraction composition of the present disclosure includes rosmarinic acid. From the viewpoint of skin efficacy, the concentration (content, ppm) of rosmarinic acid in the extract composition of the present disclosure is preferably 50 ppm or more, more preferably 100 ppm or more, and even more preferably 150 ppm or more. In the present disclosure, the concentration of rosmarinic acid can be measured using high performance liquid chromatography (HPLC), specifically, by the method described in the Examples. In this disclosure, "ppm" is based on mass, and 1 ppm is 0.0001 mass% (the same applies hereinafter).

[0033] (Sugars) The extraction composition of the present disclosure may, in one or more embodiments, include sugars, and in one or more other embodiments, may be sugar-free. The sugar may be at least one selected from glucose, fructose, and sucrose, and may be one type of sugar or a combination of two or more types of sugars. From the viewpoint of storage stability, the concentration (content, ppm) of sugars in the extract composition of the present disclosure is preferably 3000 ppm or less, more preferably 2500 ppm or less, 1000 ppm or less, or even more preferably 500 ppm or less. In the present disclosure, the concentrations of glucose, fructose, and sucrose can be measured using high performance liquid chromatography (HPLC), specifically by the method described in the Examples. In one or more embodiments, the sugar concentration can be the concentration of glucose, fructose, or sucrose, or the total concentration of glucose, fructose, and sucrose in combination.

[0034] (mass ratio) From the viewpoint of improving storage stability, the mass ratio of sugars to rosmarinic acid (sugars / rosmarinic acid) in the extract composition of the present disclosure is 10 or less, preferably 8.0 or less, more preferably 5.0 or less, even more preferably 3.0 or less, even more preferably 1.5 or less, even more preferably 1.0 or less, and even more preferably 0.8 or less.

[0035] In one or more embodiments, the extraction composition of the present disclosure may be further purified to produce a purified product. Therefore, in one or more embodiments, the method for producing the extraction composition of the present disclosure may further include a step of purifying the extract composition.

[0036] In one or more embodiments, the extraction composition of the present disclosure may be used as is, or may be diluted, concentrated, or dried before use. For example, an extract may be obtained by soaking a plant in an extracting solvent other than water, and then the extract may be adjusted using only the extracting solvent other than water so that the rosmarinic acid concentration and solvent concentration in the extract reach predetermined levels, or the extract may be prepared using the extracting solvent other than water and water, and used as the extract composition.

[0037] [Uses of the extract composition] In one or more embodiments, the extraction composition of the present disclosure can be used to promote stratum corneum formation or epidermal turnover. Thus, in another aspect, the present disclosure relates to a method for promoting stratum corneum formation using the extraction composition of the present disclosure. In another aspect, the present disclosure relates to a method for promoting epidermal turnover using the extraction composition of the present disclosure. In one or more embodiments, the extract composition of the present disclosure can be used as an active ingredient in a composition for promoting stratum corneum formation or epidermal turnover, or can be used to produce such a composition. Therefore, in one aspect, the present disclosure relates to a composition containing a plant extract component, which contains rosmarinic acid and has a mass ratio of sugars to rosmarinic acid (sugars / rosmarinic acid) of not more than 10. Preferred values ​​for the content of rosmarinic acid, the content of sugars, and the mass ratio of sugars to rosmarinic acid in the composition of this aspect are the same as those for the extract composition of the present disclosure described above. In one or more embodiments, the composition of this aspect is a composition for promoting stratum corneum formation or epidermal turnover. The composition of this embodiment can be suitably used as an external product or food. In the present disclosure, examples of external products include cosmetics, pharmaceuticals, quasi-drugs, bath additives, and perfumed products such as toothpaste. In another aspect, the present disclosure relates to an external product or food containing the composition of the present disclosure. In one or more embodiments, the extract composition of the present disclosure can be suitably used in cosmetics. That is, in one aspect, the present disclosure relates to a cosmetic containing the extract composition of the present disclosure. Examples of the cosmetic include hair cosmetics and skin cosmetics. Examples of product forms of hair cosmetics include hair shampoo, hair conditioner, and hair treatment. Examples of product forms of skin cosmetics include facial cleanser, moisturizer, emulsion, and serum. In another aspect, the present disclosure relates to a cosmetic containing the extract composition of the present disclosure. When the extract composition of the present disclosure or the composition of this embodiment is incorporated into a skin topical preparation such as a cosmetic, effects such as improving the skin's moisture retention ability, preventing and improving rough skin, reducing the formation of wrinkles and texture marks, and improving firmness can be expected. Examples of the form of topical products include creams, liquid lotions, milky lotions, sprays, and skin lotions. The content of the extract composition of the present disclosure or the composition of this embodiment in topical products or foods is preferably 0.0001% or more, more preferably 0.01% or more, even more preferably 1% or more, and preferably 10% or less, more preferably 5% or less. [Example]

[0038] The present disclosure will be described in more detail below with reference to examples, but these are merely illustrative examples and the present disclosure is not limited to these examples.

[0039] [Production Example of Extract Composition of Example 1 (Thin Film Hydroponic)] The extract composition of Example 1 was obtained from rosemary grown hydroponically (thin film hydroponics). Below are the methods for growing, harvesting, and extracting rosemary. (1) Hydroponic cultivation [Seeding] Seed source: Sakata Seed Sowing method: Cover a 288-hole plug tray with Baydon (product name, manufactured by Mitsubishi Chemical Aqua Solutions Co., Ltd.) and sow rosemary seeds. Watering: Once a day Nutrient solution: Tap water until germination. After germination, water with the following nutrient solution. [Planting] Planting: Remove the Baydon medium from the plug tray and plant in the planting hole. Planting hole spacing: 45 plants / 45 holes [Growing bed] Cultivation equipment: Napperland (product name, manufactured by Mitsubishi Chemical Aqua Solutions Co., Ltd.) Daily cumulative solar radiation: 4.764MJ / m 2 (average for one year) Maximum solar radiation intensity: 0.338kW / m 2 (average for one year) [Nutrient solution composition] The EC of the nutrient solution was measured once a day using a pH / EC meter (Hanna Instruments HI98131) and diluted with tap water to an EC of 1.0 (mS / cm). The nutrient solution was completely replaced once a month. High-Tempo Ar:High-Tempo Cu (Mitsubishi Chemical Aqua Solutions) was mixed in a 3:1 ratio while diluting with tap water, and diluted with tap water until the EC reached 1.0 (mS / cm). [Nutrient solution supply method] After filling the tank with nutrient solution, it is pumped to the Napperland cultivation beds. After flowing over the cultivation beds, the nutrient solution returns to the tank and is pumped again to circulate. When the water volume drops to a certain level, it is automatically replenished. [pH of nutrient solution] The pH of the nutrient solution was measured once a day with a pH meter and adjusted to 5.7 using a pH adjuster. pH adjuster: sulfuric acid [House temperature (cultivation temperature)] 24-hour average temperature: 15.9~32.7℃ Average daytime (light season) temperature: 18.7 to 37.7°C Average nighttime (dark period) temperature: 12.5-27.3°C [Cultivation period] Cultivation period in the main field: 3 months (July to September 2022) (2) Harvest Only leaves of hydroponically grown rosemary were harvested. (3) Pre-extraction treatment Harvested rosemary leaves were blanched (steamed). Blanching was performed on the day of harvest. <Branching process> Equipment: Steam convection oven (CSVH-E10-T, Comet Kato) Blanching temperature: 110℃ Blanching time: 15 minutes (4) Drying After pre-extraction treatment, the rosemary leaves were dried. Equipment: Constant temperature dryer (DRR420DA, ADVANTEC) Drying temperature: 60℃ Drying time: 18 hours (5) Extraction The dried plant was immersed in a 10% (v / v) butylene glycol aqueous solution (extraction solvent) and extracted for 24 hours at 25°C to obtain an extract. The amount of extraction solvent used was 25 times the dry weight of the rosemary. The extract composition of Example 1 was prepared using butylene glycol and water so that the rosmarinic acid concentration of the above extract was 300 ppm and the solvent concentration was 50% (v / v) butylene glycol aqueous solution.

[0040] [Production Example of Extract Composition of Example 2 (Submerged Hydroponic Culture)] The extract composition of Example 2 was obtained from rosemary grown hydroponically (submerged hydroponics). Below are the methods for growing, harvesting, and extracting rosemary. (1) Hydroponic cultivation [Seeding] Seed source: Sakata Seed Sowing method: Cover a 288-hole plug tray with Baydon (product name, manufactured by Mitsubishi Chemical Aqua Solutions Co., Ltd.) and sow rosemary seeds. Watering: Once a day Nutrient solution: Tap water until germination. After germination, water with the following nutrient solution. [Planting] Planting: Remove the Baydon medium from the plug tray and plant in the planting hole. Planting hole spacing: 10 plants / 10 holes [Growing bed] Cultivation equipment: LAPOND hydroponic cultivation system Daily cumulative solar radiation: 4.764 MJ / m2 (annual average) Maximum solar radiation intensity: 0.338kW / m2 (annual average) [Nutrient solution composition] The EC of the nutrient solution was measured once a day using a pH / EC meter (Hanna Instruments HI98131) and diluted with tap water to an EC of 1.0 (mS / cm). The nutrient solution was completely replaced once a month. High-Tempo Ar:High-Tempo Cu (Mitsubishi Chemical Aqua Solutions) was mixed in a 3:1 ratio while diluting with tap water, and diluted with tap water until the EC reached 1.0 (mS / cm). [Nutrient solution supply method] The nutrient solution is supplied so that all or part of the plant's roots are submerged (about 50 mm deep). The nutrient solution is supplied continuously, and any overflow is collected and circulated. Air is also periodically supplied to the nutrient solution using an air pump. [pH of nutrient solution] The pH of the nutrient solution was measured once a day with a pH meter and adjusted to 5.7 using a pH adjuster. pH adjuster: sulfuric acid [House temperature (cultivation temperature)] 24-hour average temperature: 15.9~32.7℃ Average daytime (light season) temperature: 18.7 to 37.7°C Average nighttime (dark period) temperature: 12.5-27.3°C [Cultivation period] Cultivation period in the main field: 3 months (July to September 2022) (2) Harvest Only leaves of hydroponically grown rosemary were harvested. (3) Pre-extraction treatment Harvested rosemary leaves were blanched (steamed). Blanching was performed on the day of harvest. <Branching process> Equipment: Steam convection oven (CSVH-E10-T, Comet Kato) Blanching temperature: 110℃ Blanching time: 15 minutes (4) Drying After pre-extraction treatment, the rosemary leaves were dried. Equipment: Constant temperature dryer (DRR420DA, ADVANTEC) Drying temperature: 60℃ Drying time: 18 hours (5) Extraction The dried plant was immersed in a 10% (v / v) butylene glycol aqueous solution (extraction solvent) and extracted for 24 hours at 25°C to obtain an extract. The amount of extraction solvent used was 25 times the dry weight of the rosemary. The extract composition of Example 2 was prepared using butylene glycol and water so that the rosmarinic acid concentration of the above extract was 300 ppm and the solvent concentration was 50% (v / v) butylene glycol aqueous solution.

[0041] [Production Example of Extract Composition of Example 3 (Spray Cultivation)] The extract composition of Example 3 was obtained from rosemary grown hydroponically (spray grown). Below are the methods for growing, harvesting, and extracting rosemary. (1) Raw materials Rosemary grown by spray cultivation was purchased from the following source. Plant source: Myoko Garden Co., Ltd. (2) Pre-extraction treatment Purchased rosemary leaves were blanched (steamed). The blanching was performed on the day of purchase. <Branching process> Equipment: Steam convection oven (CSVH-E10-T, Comet Kato) Blanching temperature: 110℃ Blanching time: 15 minutes (4) Drying After pre-extraction treatment, the rosemary leaves were dried. Equipment: Constant temperature dryer (DRR420DA, ADVANTEC) Drying temperature: 60℃ Drying time: 18 hours (5) Extraction The dried plant was immersed in a 10% (v / v) butylene glycol aqueous solution (extraction solvent) and extracted for 24 hours at 25°C to obtain an extract. The amount of extraction solvent used was 25 times the dry weight of the rosemary. The extract composition of Example 3 was prepared using butylene glycol and water so that the rosmarinic acid concentration of the above extract was 300 ppm and the solvent concentration was 50% (v / v) butylene glycol aqueous solution.

[0042] [Production Examples of Extract Compositions of Examples 4 to 9 (Thin Film Hydroponic)] Extract compositions were obtained by hydroponic cultivation (thin film hydroponic cultivation), harvesting, pre-extraction treatment, drying, and extraction in the same manner as in Example 1. Fructose was then added in the amounts (ppm) shown in Table 1 so that the total sugar content in the extract compositions reached the concentrations shown in Table 1, to give the extract compositions of Examples 4 to 9.

[0043] [Production Example of Extract Composition of Comparative Example 1 (Soil Cultivation)] An extract composition of Comparative Example 1 was obtained from rosemary grown outdoors. Below are the methods for growing, harvesting, and extracting rosemary. (1) Soil cultivation (open field cultivation) Seed source: Sakata Seed Cultivation soil: Herb soil (Prothleif) The seeds were sown in soil and cultivated by watering once a day. (2) Harvest Only cultivated rosemary leaves were harvested. (3) Pre-extraction treatment Harvested rosemary leaves were blanched and then dried to obtain dried plants. Blanching was performed on the day of harvest. <Branching process> Equipment: Steam convection oven (CSVH-E10-T, Comet Kato) Blanching temperature: 110℃ Blanching time: 15 minutes (4) Drying After pre-extraction treatment, the rosemary leaves were dried. Device: Drying temperature: 60℃ Drying time: 18 hours (5) Extraction The dried plant was immersed in a 10% (v / v) butylene glycol aqueous solution (extraction solvent) and extracted for 24 hours at 25°C to obtain an extract. The amount of extraction solvent used was 25 times the dry weight of the rosemary. The extract composition of Comparative Example 1 was prepared using butylene glycol and water so that the rosmarinic acid concentration of the above extract was 300 ppm and the solvent concentration was 50% (v / v) butylene glycol aqueous solution.

[0044] [Measurement of rosmarinic acid concentration in the extract composition] The concentration of rosmarinic acid was measured using high performance liquid chromatography (HPLC) under the following measurement conditions. <Measurement conditions> Equipment: Ultimate 3000 (HPLC), Corona Ultra (detector) (manufactured by Dionex) Column: InertSustain ODS-3 5um 3.0x150mm (GL Sciences) Eluent: 0.1% formic acid (Solution A) and acetonitrile (Solution B) Inclination conditions: from 95:5 for A liquid to 10:90 for B liquid Flow rate: 0.5mL / min Column temperature: 40℃ Detector: UV Standard: Rosmarinic acid (Fujifilm Wako Pure Chemical Industries, Ltd.)

[0045] [Measurement of sugar concentration in the extract composition] The concentrations of sugars (glucose, fructose, sucrose) were measured using high performance liquid chromatography (HPLC) under the following measurement conditions. In Table 1, the total amount of sugars means the sum (total concentration) of glucose, fructose, and sucrose. <Measurement conditions> Equipment: Ultimate 3000 (HPLC), Corona Ultra (detector) (manufactured by Dionex) Column: InertSustain ODS-3 5um 3.0x150mm (GL Sciences) Eluent: 0.1% formic acid (Solution A) and acetonitrile (Solution B) Inclination conditions: from 95:5 for A liquid to 10:90 for B liquid Flow rate: 0.5mL / min Column temperature: 40℃ Detector:CAD Standard: Glucose (Fujifilm Wako Pure Chemical Industries, Ltd.) Fructose (Fujifilm Wako Pure Chemical Industries, Ltd.) Sucrose (Fujifilm Wako Pure Chemical Industries, Ltd.)

[0046] [Evaluation of storage stability] The following coloring test was carried out to evaluate the storage stability of the extract composition. <Coloring test> The coloring of the extract composition before and after storage was evaluated using absorbance. The absorbance (wavelength: 600 nm) of the prepared extract composition was measured using an absorption spectrophotometer (device name: U-2910, manufactured by Hitachi High-Technologies Corporation) (absorbance before storage). After measuring the absorbance before storage, the extract composition was stored in a glass tube and allowed to stand in a thermostatic bath at 55°C for one week. After standing for one week, the absorbance (wavelength: 600 nm) of the extract composition was measured in the same manner as the absorbance before storage (absorbance after storage). The absorbance retention rate was calculated using the following formula: The higher the absorbance retention rate, the more excellent the storage stability can be evaluated. Absorbance maintenance rate = [(Absorbance before storage) / (Absorbance after storage) x 100] The results are shown in Table 1.

[0047] [Table 1]

[0048] As shown in Table 1, the extract compositions of Examples 1 to 9, which were extracted from hydroponically grown rosemary and had a mass ratio (sugars / rosmarinic acid) of 0.79 to 10, had a higher absorbance retention rate than Comparative Example 1, which was extracted from outdoor-grown rosemary and had a mass ratio (sugars / rosmarinic acid) of 12.42, and were therefore able to reduce discoloration that occurs over time and had excellent storage stability. [Industrial Applicability]

[0049] According to one aspect of the present disclosure, a method for producing a plant-derived extract composition having excellent storage stability can be provided.

Claims

1. A method for producing a plant-derived extract composition, comprising: A method for producing a plant-derived extract composition, comprising a step of obtaining the extract composition from hydroponically grown rosemary.

2. 2. A method for producing the plant-derived extract composition according to claim 1, comprising a step of blanching hydroponically grown rosemary prior to extraction.

3. The method for producing a plant-derived extract composition according to claim 1, wherein the nutrient solution used in the hydroponic cultivation is maintained at a pH of 7.5 or less.

4. 2. The method for producing a plant-derived extract composition according to claim 1, wherein the hydroponic culture is one of thin film hydroponic culture, submerged hydroponic culture, and spray hydroponic culture, or a combination thereof.

5. 2. The method for producing a plant-derived extract composition according to claim 1, wherein the electrical conductivity (EC) of the nutrient solution used in the hydroponic cultivation is maintained at 0.5 mS / cm or more and 2.0 mS / cm or less.

6. The method for producing a plant-derived extract composition according to claim 2, wherein the blanching treatment is one or more of a boiling treatment, a microwave treatment, and a steam treatment.

7. A plant-derived extract composition containing rosmarinic acid, in which the mass ratio of sugars to rosmarinic acid (sugars / rosmarinic acid) is 10 or less.

8. 8. The plant-derived extract composition of claim 7, wherein the plant is rosemary.

9. 8. The plant-derived extract composition according to claim 7, wherein the concentration of rosmarinic acid in the extract composition is 50 ppm or more.

10. A cosmetic comprising the plant-derived extract composition according to any one of claims 7 to 9.

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