Biological fluid analyzer with adaptive aperture device - Patents.com

Abstract

A biological fluid analyzer is provided, the biological fluid analyzer comprising: an imaging system, the imaging system comprising: a light source assembly configured to emit light; a first lens assembly configured to direct light from the light source assembly; and an aperture device configured to apply an aperture to allow light from the first lens assembly to pass through the aperture to indirectly define a probing light, the imaging system configured to receive the probing light and defining a probing volume configured to receive a container including a prepared biological fluid sample, the biological fluid analyzer comprising: a controller configured to control the aperture device according to the aperture configuration, the aperture device configured to apply an aperture having a first aperture configuration in response to a first aperture control signal, and the aperture device configured to apply an aperture having a second aperture configuration in response to a second aperture control signal.

Description

[Technical Field]

[0001] The present invention relates to the analysis of biological fluid samples, such as blood samples or cell culture samples, and to tools, methods, and systems used to perform such biological fluid sample analysis. More particularly, the present invention relates to biological fluid analyzers for analyzing biological fluids. [Background technology]

[0002] Analysis of biological fluid samples, such as the determination of blood or cell culture parameters, can be lengthy and can require a significant number of preparatory steps, resources, and highly specialized equipment. Summary of the Invention [Problem to be solved by the invention]

[0003] Therefore, there may be a need for a biological fluid analyzer that can enable improved analysis of biological fluid samples. [Means for solving the problem]

[0004] Accordingly, a biological fluid analyzer is provided. The biological fluid analyzer includes an imaging system. The imaging system includes a light source assembly configured to emit light. The imaging system includes a first lens assembly configured to direct the light from the light source assembly. The imaging system is configured to receive the probing light and defines a probing volume configured to receive a container containing a prepared biological fluid sample. The biological fluid analyzer can include a controller.

[0005] The controller may be configured to control the imaging system according to the probing light configuration. For example, the controller may be configured to provide a first probing light control signal to the imaging system to apply a first probing light configuration within the imaging device. For example, the controller may be configured to provide a second probing light control signal to the imaging system to apply a second probing light configuration within the imaging device. The imaging device is configured to provide a first probing light when applying the first probing light configuration, and to provide a second probing light when applying the second probing light configuration, the second probing light having a different incident angle and / or incident angle range than the first probing light.

[0006] The imaging system can include an aperture device configured to apply an aperture to allow light from the first lens assembly to pass through the aperture to indirectly define the probing light.

[0007] The controller may be configured to control the aperture device according to the aperture configuration. The aperture device may be configured to apply an aperture having a first aperture configuration in response to a first aperture control signal. In other words, the first probing light control signal may include a first aperture control signal. The aperture device may be configured to apply an aperture having a second aperture configuration in response to a second aperture control signal. In other words, the second probing light control signal may include a second aperture control signal.

[0008] A biological fluid analyzer can be beneficial in providing more efficient analysis of biological fluid samples. For example, more precise, robust, and rapid image-based biological fluid parameter determination or estimation may be achieved. An example of a biological fluid parameter is a blood parameter that is platelet concentration in a blood sample. The determination or estimation of a biological fluid parameter may be useful, for example, for blood cell classification, such as determining, classifying, or estimating cell types in a blood sample, or for blood cell classification, such as determining, classifying, or estimating cell types in a cell culture sample. The biological fluid analyzer may enable rapid cell classification with greater accuracy and reliability without having to rely on more expensive optical elements.

[0009] By providing an aperture device and a controller configured to control the aperture device, the biological fluid analyzer can advantageously control and adjust the characteristics of the probing light without the need for customization of more complex components of the imaging system, such as the light source assembly, the first lens assembly, the second lens assembly, any other suitable component of the imaging system, or a suitable combination thereof. In other words, the biological fluid analyzer can provide an inexpensive and easy-to-maintain solution that enables adjustment of the characteristics of the probing light.

[0010] By providing a controller, customization of the biological fluid analyzer can be achieved. For example, by providing an aperture device and a controller configured to control the aperture device, the light source assembly, or both the aperture device and the light source assembly, the biological fluid analyzer can advantageously control and adjust the characteristics of the probing light. Because the characteristics of the probing light can affect the determination or estimation of biological fluid parameters within the biological fluid sample, customization of the probing light can advantageously provide more information about the biological fluid sample, such as information for determining or estimating cell types within the biological fluid sample, without the need for dedicated optical elements.

[0011] The above and other features and advantages of the present disclosure will become readily apparent to those skilled in the art from the following more detailed description of exemplary embodiments of the present disclosure, taken in conjunction with the accompanying drawings. [Brief explanation of the drawings]

[0012] [Figure 1] 1 is a schematic longitudinal cross-sectional view of a biological fluid analyzer in which the aperture device has a second aperture configuration. [Figure 2] 2 is a schematic diagram of the biological fluid analyzer of FIG. 1 in which the aperture device has a first aperture configuration; [Figure 3] 1 is a diagram illustrating a schematic view of an aperture device including a blade and a blade aperture. [Figure 4] 1 shows a schematic diagram of an aperture device comprising a plate; [Figure 5] 1A and 1B are schematic diagrams illustrating an aperture device comprising a glass substrate having a first transparency pattern. [Figure 6] 6 shows a schematic diagram of the aperture device of FIG. 5 in the case where the glass substrate has a second transparent configuration. [Figure 7] FIG. 10 is a schematic diagram of a biological fluid analyzer in which the light source assembly has a second light configuration. [Figure 8] 8 is a schematic diagram of the biological fluid analyzer of FIG. 7 in which the light source assembly has a first light configuration. [Figure 9] FIG. 2A is a diagram illustrating a schematic of a light source assembly having a first geometric pattern corresponding to a first light configuration. [Figure 10] 10 is a schematic diagram of the light source assembly of FIG. 9 in the case where the light source assembly has a second geometric pattern corresponding to a second light configuration. DETAILED DESCRIPTION OF THE INVENTION

[0013] Various example biological fluid analyzers are described below with reference to the figures where relevant. It should be noted that the figures may or may not be drawn to scale. It should also be noted that the figures are intended only to facilitate the description of various embodiments. The figures are not intended as an exhaustive description of the invention or as limitations on the scope of the invention. Moreover, the illustrated embodiments need not have all aspects or advantages shown.

[0014] Aspects or advantages described in connection with a particular embodiment are not necessarily limited to that embodiment and may be implemented in any other embodiment even if not so shown or explicitly described.

[0015] The figures are schematic and simplified for clarity, merely showing details that aid in understanding the present disclosure, while omitting other details. The same reference numerals are used throughout for identical or corresponding parts.

[0016] In one or more example biological fluid analyzers, a biological fluid analyzer is provided, the biological fluid analyzer comprising an imaging system, the imaging system comprising: a light source assembly configured to emit light; a first lens assembly configured to direct light from the light source assembly; and an aperture device configured to apply an aperture to allow light from the first lens assembly to pass through the aperture to indirectly define a probing light, the imaging system configured to receive the probing light and defining a probing volume configured to receive a container including a prepared biological fluid sample, the biological fluid analyzer comprising: a controller configured to control the aperture device according to a first configuration, the aperture device configured to apply an aperture having a first aperture configuration in response to a first aperture control signal, and the aperture device configured to apply an aperture having a second aperture configuration in response to a second aperture control signal.

[0017] The biological fluid analyzer can be a blood analyzer. In other words, the biological fluid analyzer can be configured to analyze human blood and / or animal blood, such as mammalian blood. Thus, the prepared biological fluid sample can be a prepared blood sample.

[0018] The biological fluid analyzer can be a cell culture analyzer. In other words, the biological fluid analyzer can be configured to analyze cell cultures, such as cultures of cells derived from multicellular eukaryotes, e.g., mammalian cells, animal cells, and / or human cells, and / or cultures of cells grown from plant tissue cultures, fungal cultures, and / or (pathogenic) microbial cultures. Thus, the prepared biological fluid sample can be a prepared cell culture sample.

[0019] Further provided is a method of operating a biological fluid analyzer, such as the biological fluid analyzers disclosed herein, the method including emitting light, e.g., by a light source assembly; directing the light, e.g., by a first lens assembly; applying an aperture to indirectly define a probing light, e.g., to allow light from the first lens assembly to pass through the aperture; and receiving the probing light within a probing volume, wherein applying the aperture includes applying an aperture having a first aperture configuration, e.g., in response to a first aperture control signal from a controller, and applying an aperture having a second aperture configuration, e.g., in response to a second aperture control signal from the controller.

[0020] As used herein, an "imaging system" refers to a system comprising optical elements configured to enable a graphical representation of a body sample or a parameter of the body sample. For example, in a biological fluid analyzer, the imaging system may enable a graphical representation of cell types contained within a prepared blood sample or cell culture analyzed by the biological fluid analyzer.

[0021] The light source assembly may be formed from a support element that supports multiple light sources, such as multiple LEDs. The light source assembly may include electrical circuitry that provides power to the multiple light sources. A lens assembly, such as one or more of the first lens assembly, the second lens assembly, and the third lens assembly, may be formed from one or more lenses. The one or more lenses of the lens assembly may be arranged to direct received light according to a desired light pattern. For example, the lens assembly may be arranged to direct received light toward a back focal point of the lens assembly. Configuring lens assemblies to achieve a desired light pattern is well known. This may be achieved by an appropriate combination of lenses, such as convex and concave lenses, having appropriate refractive indices.

[0022] The aperture device can apply an aperture to allow light from the first lens assembly to pass through the aperture. In other words, the aperture device can block a portion of the light directed from the first lens assembly. The aperture device can therefore advantageously filter light downstream of the aperture device by selecting the light that passes through the applied aperture.

[0023] The controller may be configured to change the aperture configuration according to the aperture control signal. In other words, the controller may apply apertures according to at least a first aperture configuration and a second aperture configuration. The controller may also apply apertures according to a further aperture configuration different from the first aperture configuration and the second aperture configuration. For example, the aperture device may be configured to apply apertures having a third aperture configuration in response to a third aperture control signal.

[0024] By providing different aperture configurations, the characteristics of the light passing through the applied aperture can be customized. The light passing through the applied aperture indirectly defines the probing light. The term "to indirectly define" explains that the light passing through the applied aperture must be directed by one or more optical elements downstream of the aperture device to form the probing light.

[0025] As used herein, "probing light" is light configured to be received by a probing volume configured to receive a container containing a prepared blood sample or cell culture. In other words, the probing light is configured to be received by a container containing a prepared blood sample or cell culture when the container is received within the probing volume.

[0026] By changing the aperture configuration, the characteristics of the probing light change accordingly. For example, a first aperture configuration can produce probing light with a first angle of incidence while blocking probing light with a second angle of incidence. A second aperture configuration can produce probing light with both the first and second angles of incidence.

[0027] Because the characteristics of the probing light can affect the determination or estimation of blood parameters in a prepared blood sample or the determination or estimation of cell culture parameters in a cell culture sample, customizing the probing light can advantageously provide more information about a blood sample or cell culture sample, such as information about cell types within the blood sample or cell culture sample, in a robust and rapid manner without the need for additional dedicated optical elements. For example, a probing light having a first incident angle can be suitable for determining or estimating a first cell type. A probing light having a second incident angle can be suitable for determining or estimating a second cell type. In other words, probing lights with different incident light settings / incident angles can enable improved cell classification based on the respective image data of cells with different incident light settings.

[0028] The controller may be configured to control the light source assembly according to the light configuration. The light source assembly may be configured to emit light according to the first light configuration in response to a first light control signal. In other words, the first probing light control signal may include a first light control signal. The light source assembly may be configured to emit light according to the second light configuration in response to a second light control signal. In other words, the second probing light control signal may include a second light control signal.

[0029] By providing a controller configured to control the aperture device and the light source assembly, the biological fluid analyzer can advantageously be enabled to adapt the adjustment of the probing light to the characteristics of the biological fluid analysis, for example, by relying on control of the light source assembly or of both the light source assembly and the aperture device simultaneously when precise adjustment of the probing light is required, and by relying on control of the aperture device when cost efficiency is desired.

[0030] In one or more example biological fluid analyzers, the first probing light provided in response to a first probing light control signal, such as a first aperture control signal and / or a first light control signal, can include light having an incident light angle relative to the optical axis less than a first upper light angle. The first upper light angle can be 15 degrees or less, e.g., 10 degrees or less, or even 5 degrees or less. In one or more example biological fluid analyzers, the first probing light provided in response to a first probing light control signal, such as a first aperture control signal and / or a first light control signal, can be free of, or substantially free of, light having an incident light angle relative to the optical axis greater than the first upper light angle.

[0031] In one or more example biological fluid analyzers, the first probing light provided in response to a first probing light control signal, such as a first aperture control signal and / or a first light control signal, can include light having an incident light angle relative to the optical axis greater than a first lower limit light angle. The first lower limit light angle can be 0 degrees or greater, e.g., 2 degrees or greater, 5 degrees or greater, or 10 degrees or greater. In one or more example biological fluid analyzers, the first probing light provided in response to a first probing light control signal, such as a first aperture control signal and / or a first light control signal, can be free of, or substantially free of, light having an incident light angle relative to the optical axis less than the first lower limit light angle.

[0032] The first lower limit optical angle and the first upper limit optical angle can define a first optical angle range of the first probing light. The first optical angle range can be 0 degrees to 10 degrees, for example, 0 degrees to 15 degrees, such as 5 degrees to 10 degrees.

[0033] In other words, the imaging system may be configured to selectively provide a first probing light that includes light having only incident light angles within a first light angle range. In one or more example biological fluid analyzers, the second probing light provided in response to a second probing light control signal, such as a second aperture control signal and / or a second light control signal, can include light having an incident light angle relative to the optical axis that is less than a second upper light angle. The second upper light angle can be 30 degrees or less, e.g., 20 degrees or less, or even 17 degrees or less. In one or more example biological fluid analyzers, the second probing light provided in response to a second probing light control signal, such as a second aperture control signal and / or a second light control signal, can be free of, or substantially free of, light having an incident light angle relative to the optical axis that is greater than the second upper light angle.

[0034] In one or more example biological fluid analyzers, the second probing light provided in response to a second probing light control signal, such as a second aperture control signal and / or a second light control signal, can include light having an incident light angle relative to the optical axis greater than a second lower limit light angle. The second lower limit light angle can be greater than the first upper limit light angle. The second lower limit light angle can be 0 degrees or greater, e.g., 2 degrees or greater, 5 degrees or greater, or 10 degrees or greater. In one or more example biological fluid analyzers, the second probing light provided in response to a second probing light control signal, such as a second aperture control signal and / or a second light control signal, can be free of, or substantially free of, light having an incident light angle relative to the optical axis less than the second lower limit light angle.

[0035] The second lower limit optical angle and the second upper limit optical angle can define a second optical angle range of the second probing light. The second optical angle range can be 0 to 20 degrees, such as 5 to 20 degrees, e.g., 10 to 17 degrees. The second optical angle range and the first optical angle range can be non-overlapping.

[0036] In other words, the imaging system may be configured to selectively provide a second probing light that includes light having only incident light angles within a second light angle range. In one or more example biological fluid analyzers, the first aperture configuration defines a first probing volume and the second aperture configuration defines a second probing volume, with at least a portion of the first and second probing volumes overlapping. In some examples, the first and second probing volumes overlap by at least 90%, at least 70%, or at least 50%. The biological fluid analyzer can be configured to maintain the probing volume, and possibly the container as well, at a steady state during a transition from, for example, the first aperture configuration to the second aperture configuration. Additionally or alternatively, this allows the aperture device to image at least a portion of the same volume of prepared biological fluid in the first aperture configuration and the second aperture configuration. This may be particularly advantageous in combination with an example biological fluid analyzer, where the imaging system is configured to selectively provide a first probing light that includes light having only incident light angles within a first light angle range, and / or the imaging system is configured to selectively provide a second probing light that includes light having only incident light angles within a second light angle range, as this allows for the effective acquisition of image data of at least a portion of the same volume of the prepared biological fluid sample acquired at different incident light angles.

[0037] In one or more example biological fluid analyzers, the biological fluid analyzer defines an optical path corresponding to the optical path of a transmission microscope. In other words, the imaging system can be configured to obtain image data from probing light transmitted through the probing volume. This allows for imaging of prepared bodily fluid samples without having to rely solely on fluorescence emitted by the prepared fluid analyzer. Furthermore, this can allow for increased simplicity and a reduced footprint of the biological fluid analyzer, which may be particularly useful for cell classification, especially in point-of-care and / or clinical settings. Furthermore, it can allow the biological fluid analyzer to obtain an image stack of image planes for richer image data and possibly improved cell classification.

[0038] Thus, in one or more example biological fluid analyzers, a biological fluid analyzer is provided, the biological fluid analyzer comprising: an imaging system, a light source assembly configured to emit light; and a first lens assembly configured to direct light from the light source assembly to directly or indirectly define a probing light, the imaging system configured to receive the probing light and defining a probing volume configured to receive a container containing a prepared biological fluid sample; the biological fluid analyzer comprises a controller configured to control the light source assembly according to a light configuration, the light source assembly configured to emit light according to a first light configuration in response to a first light control signal, and the light source assembly configured to emit light according to a second light configuration different from the first light configuration in response to a second light control signal.

[0039] By providing a controller that can be configured to control the light source assembly, the biological fluid analyzer can advantageously control and adjust the characteristics of the probing light in a wide manner, i.e., the probing light can be adjusted according to a wider range of probing light parameters and in a more precise manner, which can be useful for determining or estimating a greater amount of blood parameters, which can improve the determination or estimation of cell types within a blood sample.

[0040] The light directed by the first lens assembly directly or indirectly defines the probing light. The term "to indirectly define" means that the light directed by the first lens assembly must be directed by one or more optical elements downstream of the first lens assembly to form the probing light. The term "to directly define" means that the light directed by the first lens assembly forms the probing light in the absence of optical elements downstream of the first lens assembly.

[0041] The controller may be configured to control the light source assembly via a light control signal. In other words, the controller may control the light source assembly according to at least a first light configuration and a second light configuration. The controller may also control the light source assembly according to an additional light configuration different from the first light configuration and the second light configuration. For example, the light source assembly may be configured to emit light according to a third light configuration in response to a third light control signal from the controller.

[0042] By providing different light configurations, the characteristics of the light emitted by the light sources of the light source assembly can be customized. The light directed from the first lens assembly is received from the light source assembly. Therefore, when the light directed by the first lens assembly directly or indirectly defines the probing light, the probing light depends on the optical configuration of the light emitted by the first light source assembly. By changing the optical configuration of the light source assembly, the characteristics of the probing light can be correspondingly changed. For example, the first optical configuration can produce probing light having a first angle of incidence. The second aperture configuration can produce probing light having a first angle of incidence and a second angle of incidence.

[0043] Because the characteristics of the probing light can affect the determination or estimation of biological fluid parameters in the prepared biological fluid sample, customizing the probing light can advantageously provide more information about the biological fluid sample, such as information for determining or estimating cell types within the biological fluid sample, in a reliable and rapid manner without the need for additional dedicated optical elements.

[0044] The imaging system can include an aperture device configured to apply an aperture to allow light from the first lens assembly to pass through the aperture.

[0045] In one or more example biological fluid analyzers, the imaging system includes a second lens assembly configured to direct light from the aperture device. In other words, light passing through the applied aperture is directed by the second lens assembly to form a probing light. The second lens assembly can be useful for concentrating the probing light on a probing volume. For example, the probing volume can be disposed at the back focal plane of the second lens assembly.

[0046] In one or more example biological fluid analyzers, the biological fluid analyzer includes a container configured to hold a prepared biological fluid sample. The container may be received within the probing volume. The container may also be referred to as a cuvette. The cuvette may be a multi-use or disposable cuvette. In one or more example biological fluid analyzers in which the cuvette is a multi-use cuvette, the biological fluid analyzer may be configured as a multi-use device including the necessary fluidic system and mechanisms for performing multiple measurements. Such example biological fluid analyzers may further include a solution pack including one or more solutions that the example biological fluid analyzer may be configured to administer and dispense through use of its fluidic system, and a mechanism for at least partially automatically preparing the prepared body fluid sample after aspiration, and may be configured to control the fluidic system to perform a washing program for at least the multi-use cuvette before and / or after biological fluid analysis.

[0047] A prepared biological fluid sample can include a biological fluid sample prepared by one or more reagents, chemicals, treatments, and / or processes. A prepared biological fluid sample can be a prepared blood sample, for example, a stained, e.g., chemically stained, blood sample. A prepared blood sample can include, for example, a hemolyzed blood sample, e.g., where most of the red blood cells in the blood sample have been removed. A prepared blood sample can include, for example, a blood sample that has been fixed so that substantially no cell movement occurs during image acquisition of the prepared blood sample. A prepared blood sample can be understood as a solution containing blood and one or more reagents and / or chemicals. A prepared blood sample can be understood as a separation, such as a separation blood sample. A prepared blood sample can be placed in a container, such as a cuvette. Once the container is received in the probing volume, the biological fluid analyzer can analyze the blood characteristics of the prepared blood sample.

[0048] In one or more example biological fluid analyzers, the container includes a container lens assembly configured to direct light from the aperture device. In other words, light passing through the applied aperture can be directed by the container lens assembly to form the probing light. Providing a container lens assembly can be beneficial in that, because the optical element directing the light from the aperture device is integral with the container, the container lens assembly can be adjusted to the type and position of the prepared biological fluid sample contained within the container.

[0049] In one or more example biological fluid analyzers, the aperture device includes a blade aperture. The aperture device can be configured to adjust the blade aperture as an aperture having a first aperture configuration in response to a first aperture control signal. The aperture device can be configured to adjust the blade aperture as an aperture having a second aperture configuration in response to a second aperture control signal.

[0050] Aperture blades can be an advantageous solution to efficiently and accurately apply apertures having varying configurations, such as apertures having varying sizes, shapes, or both sizes and shapes.

[0051] In one or more example biological fluid analyzers, the aperture device includes a plate having a first plate aperture and a second plate aperture different from the first plate aperture. The aperture device can be configured to apply the first plate aperture as an aperture having a first aperture configuration in response to a first aperture control signal. The aperture device can be configured to apply the second plate aperture as an aperture having a second aperture configuration in response to a second aperture control signal.

[0052] A plate having a first plate aperture and a second plate aperture can allow the arrangement to apply apertures with varying configurations that are reliable and easy to manufacture and maintain.

[0053] In one or more example biological fluid analyzers, the aperture device includes an actuator. The actuator can be configured to move the plate to a first position in response to a first aperture control signal. The actuator can be configured to move the plate to a second position in response to a second aperture control signal. The actuator can be useful for automating application of the aperture by linking a controller to the plate.

[0054] In one or more example biological fluid analyzers, the aperture device includes a glass substrate. The aperture device can be configured to apply a first transparency pattern in the glass substrate to provide apertures having a first aperture configuration in response to a first aperture control signal. The aperture device can be configured to apply a second transparency pattern in the glass substrate to provide apertures having a second aperture configuration in response to a second aperture control signal.

[0055] Providing first and second transparency patterns can provide a wide and precise selection of apertures with different aperture configurations. The glass substrate can include a liquid crystal display, which can be configured to precisely provide varying transparency patterns.

[0056] The aperture having the first aperture configuration can have a first diameter in the range of 0.5 mm to 4 mm. The aperture having the second aperture configuration can have a second diameter in the range of 2 mm to 8 mm. Such dimensions of the aperture having the first aperture configuration and the aperture having the second aperture configuration can result in variations in the probing light, which can be useful for determining or estimating multiple blood characteristics of the prepared blood sample.

[0057] The apertures having the third aperture configuration may have a third diameter in the range of 3 mm to 10 mm. Applying the aperture having a first aperture configuration can include decentering the aperture from an optical axis of the imaging system. Applying the aperture having a second aperture configuration can include decentering the aperture from the optical axis. As used herein, "to decentre the aperture from the optical axis" is understood to mean positioning the aperture offset from the optical axis.

[0058] By applying an aperture decentered from the optical axis, a probing light having characteristics suitable for determining or estimating a given blood parameter can be achieved, for example, a probing light having an incident angle that forms a specific angle different from 0 degrees with respect to the optical axis.

[0059] In one or more example biological fluid analyzers, apertures having a first aperture configuration can have a first shape that is circular, elliptical, rectangular, square, non-circular, or any other suitable shape. Apertures having a second aperture configuration can have a second shape that is circular, elliptical, rectangular, square, non-circular, or any other suitable shape. Apertures having a third aperture configuration can have a third shape that is circular, elliptical, rectangular, square, non-circular, or any other suitable shape.

[0060] In one or more example biological fluid analyzers, the biological fluid analyzer includes a third lens assembly configured to direct light from the probing volume. The third lens assembly can serve to focus light from the probing volume to a focal point downstream of the probing volume, such as the back focal point of the third lens assembly or another focal point along the back focal plane of the third lens assembly, which can facilitate interpretation of blood parameters of the prepared blood sample determined or estimated by the probing light.

[0061] In one or more example biological fluid analyzers, the biological fluid analyzer includes a light sensor configured to receive light directed by a third lens assembly. The light sensor can advantageously provide a graphical representation or image data of the prepared biological fluid sample. Preferably, the light sensor is disposed in the back focal plane of the third lens assembly. In other words, a convergence point, such as the back focal point, of the third lens assembly preferably extends along the light sensor. The light sensor can be an image sensor, such as a camera having a pixel resolution of at least 1 million pixels. In one or more example biological fluid analyzers, the light sensor is configured to be non-replaceable. By non-replaceable, it is meant that the camera or light sensor is at least not directly accessible during normal operation of the biological fluid analyzer and cannot be removed without removing a part of the biological fluid analyzer that is separate from the camera or light sensor, such as removing a panel for a light shield. This improves the simplicity of the biological fluid analyzer and allows for a relatively compact design, since it is not necessary to allow the user to easily access and replace the camera or light sensor.

[0062] In one or more example biological fluid analyzers, the first light configuration includes a first light pattern. The light source assembly can be configured to emit light having the first light pattern in response to a first light control signal. The first light pattern can include a first ring having a first inner diameter and a first outer diameter. The first light pattern can include, for example, a first circle centered on the optical axis, the first circle having a first diameter. The second light configuration can include a second light pattern. The light source assembly can be configured to emit light having the second light pattern in response to a second light control signal. The second light pattern can include a second ring having a second inner diameter and a second outer diameter. The first inner diameter can be smaller than the second inner diameter. In one or more example biological fluid analyzers, the first outer diameter can be equal to or smaller than the second inner diameter. The second light pattern can include, for example, a second circle centered on the optical axis, the second circle having a second diameter.

[0063] By appropriate arrangement of the first and second light patterns, the characteristics of the light emitted by the light source assembly can be efficiently customized. In other words, controlling the light patterns can provide probing lights with different incident light settings / incident angles, which in turn enables improved cell classification based on the respective image data of cells with different incident light settings.

[0064] In one or more example biological fluid analyzers, the light source assembly includes an array of light sources. In response to a first light control signal, the array of light sources can be configured to emit light according to a first geometric pattern corresponding to the first light pattern. In response to a second light control signal, the array of light sources can be configured to emit light according to a second geometric pattern corresponding to the second light pattern.

[0065] The use of geometric patterns to define the first and second light patterns can advantageously allow for precise and easily achieved customization of the light emitted by the light source assembly. For example, an array of light sources can be configured to emit light according to an annular arrangement disposed about the optical axis of the imaging system.

[0066] As noted above, variations in the light emitted by the light source assembly can produce corresponding variations in the probing light, which in turn allows for different imaging of cells within the prepared biological fluid sample. For example, defining the geometric pattern of the emitting light source can affect the angle of incidence of the probing light. In one example, a light source positioned at the optical axis of the imaging system can emit light to produce a probing light having a 0-degree incidence relative to the optical axis. A light source positioned at an increasing distance from the optical axis of the imaging system can produce a probing light having an increasing angle of incidence relative to the optical axis.

[0067] In one or more example biological fluid analyzers, a light source assembly includes a glass substrate. The light source assembly can be configured to apply a first transparency pattern in the glass substrate to emit light having a first light configuration in response to a first light control signal. The light source assembly can be configured to apply a second transparency pattern in the glass substrate to emit light having a second light configuration in response to a second light control signal.

[0068] Providing first and second transparency patterns can provide a wide and precise selection of different light configurations. The glass substrate can include a liquid crystal display, which can be configured to precisely provide varying transparency patterns.

[0069] In one or more example biological fluid analyzers, the first light arrangement includes a first color scheme. The light source assembly can be configured to emit light having the first color scheme in response to a first light control signal. The second light arrangement can include a second color scheme. The light source assembly can be configured to emit light having the second color scheme in response to a second light control signal. The first color scheme can be different from the second color scheme.

[0070] Having a first color scheme and a second color scheme can result in a probing light having a corresponding color scheme, which can be advantageous for determining or estimating specific blood parameters within the prepared biological fluid sample.

[0071] In one or more example biological fluid analyzers, the first light configuration includes a first intensity scheme. The light source assembly can be configured to emit light having the first intensity scheme in response to the first light control signal. The second light configuration can include a second intensity scheme. The light source assembly can be configured to emit light having the second intensity scheme in response to the second light control signal.

[0072] Having a first intensity scheme and a second intensity scheme can result in a probing light having a corresponding intensity scheme, which can be advantageous for determining or estimating a particular property within the prepared biological fluid sample.

[0073] In one or more example biological fluid analyzers, the light source assembly is configured to emit light off-axis in response to a first light control signal. The light source assembly can be configured to emit light off-axis in response to a second light control signal.

[0074] Emission of light off-axis from the optical axis can be useful to obtain probing light with appropriate characteristics, such as light with an appropriate angle of incidence. These and other features and advantages of the present invention will become more apparent in the light of the following detailed description of preferred embodiments, given by way of illustrative and non-limiting example only, with reference to the accompanying drawings, in which:

[0075] FIG. 1 illustrates a biological fluid analyzer 100 that includes an imaging system 1. The imaging system 1 includes a light source assembly 3 and a first lens assembly 4. An optical axis 2 of the imaging system 1 extends across the light source assembly 3 and the first lens assembly 4. In the embodiment of FIG. 1, the first lens assembly 4 includes a first lens 41, a second lens 42, and an optional third lens 43. The lenses 41, 42, and 43 are configured to direct light emitted by the light source assembly 3 toward a convergence point downstream of the first lens assembly 4.

[0076] 1 includes an aperture device 5 configured to apply an aperture to allow light from a first lens assembly 4 to pass through the aperture. In the first example biological fluid analyzer, the aperture device 5 extends along the back focal plane of the first lens assembly 4. A convergence point of light directed by the first lens assembly 4 is located at the back focal plane of the first lens assembly 4. Thus, the applied aperture can be selectively applied to include one or more of the convergence points.

[0077] 1, the light source assembly includes one or more of a first light source 31, a second light source 32, and a third light source 33. The first light source 31 is disposed on the optical axis 2 of the imaging system 1. The light beams emitted by each of the light sources 31, 32, and 33 converge to a convergence point on the back focal plane of the first lens assembly 4. In the configuration of FIG. 1, an applied aperture in the aperture device allows the light emitted by the three light sources 31, 32, and 33 to pass through the applied aperture.

[0078] Downstream of the aperture device 5, the imaging device 1 of the biological fluid analyzer 100 of FIG. 1 includes a second lens assembly 6. In the biological fluid analyzer 100, the second lens assembly 6 includes a first lens 62 and / or a second lens 62. The second lens assembly 6 is configured to direct light passing through an applied aperture in the aperture device 5 and focus the light onto the back focal plane of the second lens assembly 6. FIG. 1 shows that each convergence point on the back focal plane of the first lens assembly 4 results in a different angle of incidence on the back focal plane of the second lens assembly 6. Thus, the angle of incidence of light directed by the second lens assembly 6 onto the back focal plane of the second lens assembly 6 can be customized by applying the aperture of the aperture device 5.

[0079] In the embodiment of FIG. 1, a probing volume 7 is defined along the back focal plane of the second lens assembly 6. The probing volume 7 of FIG. 1 receives light having three different angles of incidence. The light received by the probing volume 7 is referred to as the probing light. Because the light passing through the applied aperture is directed by the second lens assembly 6 to define the probing light, the light passing through the applied aperture indirectly defines the probing light.

[0080] The angle or angles of incidence of the probing light may be customized by the aperture device 5. To achieve such customization, the biological fluid analyzer 100 includes a controller 8 configured to control the aperture device 5.

[0081] The biological fluid analyzer 100 includes a controller 8 configured to control components of the imaging system 1. For example, the controller 8 may be configured to control the aperture device 5 via one or more aperture control signals to the aperture device.

[0082] 1, the aperture device 5 is controlled by the controller 8 according to an aperture configuration in which the applied aperture has a second aperture configuration. In other words, the controller sent a second aperture control signal to the aperture device 5. As explained above, the second aperture configuration allows light converging to three convergence points on the back focal plane of the first lens assembly 4 to pass through the applied aperture.

[0083] Because the aperture device 5 of this embodiment extends along the back focal plane of the first lens assembly 4, the aperture device 5 can perform precise filtering of the light directed by the first lens assembly 4 by selectively applying apertures to include one or more of the convergence points located on the back focal plane of the first lens assembly 4. However, the aperture device 5 can similarly extend along a different plane than the back focal plane of the first lens assembly 4 and still perform appropriate filtering of the light directed by the first lens assembly 4.

[0084] In the embodiment of FIG. 1, the probing volume 7 and the container that may contain the prepared biological fluid sample are not displaced relative to the light source assembly 3 when the controller 8 sends the first and second aperture control signals.

[0085] 2 illustrates the biological fluid analyzer 100 of the embodiment of FIG. 1 when the controller controls the aperture device 5 according to an aperture configuration in which the applied aperture has a first aperture configuration. In other words, the controller has sent a first aperture control signal to the aperture device 5. In the configuration of FIG. 2, the applied aperture allows light emanating from the first light source 31 to pass through the applied aperture. Similarly, the applied aperture blocks light from the remaining light sources 32, 33. This configuration results in a probing light having a 0 degree angle of incidence with respect to the optical axis 2.

[0086] Customizing the probing light, such as customizing the angle of incidence of the probing light, can be useful for determining or estimating different blood parameters of the prepared biological fluid sample, such as blood cell type classification, once a container containing the prepared biological fluid sample is received within the probing volume 7.

[0087] It should be noted that the imaging system 1 of the biological fluid analyzer 100 of the embodiment of Figures 1 and 2 may include a third lens assembly 9 and optical sensor 11 (not shown in Figures 1 and 2 for simplicity) identical to those described below in Figures 7 and 8. As shown in these figures, the third lens assembly 9 and optical sensor 11 would be disposed downstream of the probing volume 7.

[0088] 3 shows an aperture device 5 comprising a blade aperture 51 defined by a plurality of blades 52. A controller 8 controls the blades 52 to adjust the blade aperture 51 according to a plurality of aperture configurations, including a first aperture configuration and a second aperture configuration. For example, the controller 8 can change the position of the blades 52 to adjust the form, shape, or both the form and shape of the blade aperture 51.

[0089] FIG. 4 shows an aperture device 5 including a plate 53. A schematic cross-sectional view of the plate 53 is shown in the upper portion of FIG. 4 within the dotted area. The plate 53 includes a first plate aperture 54 and a second plate aperture 55 that is different from the first plate aperture 54. The controller 8 is configured to apply the first plate aperture 54 as an aperture having a first aperture configuration and the second plate aperture 55 as an aperture having a second aperture configuration. In the embodiment of FIG. 4, the aperture having the second aperture configuration may be configured to allow a greater amount of light to pass through the applied aperture. This can result in a greater number of probing lights and a larger angle of incidence.

[0090] 4, aperture device 5 includes actuator 56. In response to a first aperture control signal from controller 8, actuator 56 may be configured to move plate 53 to a first position where the applied aperture is first plate aperture 54. In response to a second aperture control signal from the controller, actuator 56 may be configured to move plate 53 to a second position where the applied aperture is second plate aperture 55. Actuator 56 may effect such movement by one or both of rotating and linearly translating plate 53.

[0091] 1, 2, and 4 are described and shown with first, second, and third light sources 31, 32, 33, the biological fluid analyzer may optionally be configured with a single light source, which improves the simplicity of the biological fluid analyzer while still allowing imaging of prepared body fluid samples with different incident light angles.

[0092] FIG. 5 illustrates an aperture device 5 including a glass substrate 57. The glass substrate 57 in FIG. 5 illustrates a first transparency pattern in which a first circle 58 centered on the optical axis 2 is transparent, while two concentric annular rings 59, 60 disposed around the circle 58 are opaque. The first transparency pattern defines apertures corresponding to the transparent first circle 58 and having a first aperture configuration. The controller 8 is configured to control the transparency pattern of the glass substrate 57 by optical control / aperture control signals. For example, the controller 8 can vary the transparency of the annular ring 59 adjacent to the circle 58 in a second transparency pattern to make the annular ring 59 transparent. For example, such a second transparency pattern illustrated in FIG. 6 defines apertures having a second aperture configuration having a size larger than the apertures having the first aperture configuration. Thus, the apertures having the second aperture configuration may be configured to allow a greater amount of light to pass through the applied aperture to provide probing light with a greater range of incident angles than the first aperture configuration.

[0093] 7 shows a biological fluid analyzer 100, such as a blood analyzer or cell culture analyzer, that includes an imaging system 1. The imaging system 1 includes a light source assembly 3 and a first lens assembly 4. An optical axis 2 of the imaging system 1 extends across the light source assembly 3 and the first lens assembly 4.

[0094] 7, the light source assembly comprises a first light source 31, a second light source 32, and a third light source 33. The first light source 31 is disposed on the optical axis 2 of the imaging system 1. The second light source 32 and the third light source 33 are disposed symmetrically with respect to the optical axis 2.

[0095] In Figure 7, the first lens assembly 4 includes a front convex surface and a rear concave surface. The light beams emitted by each of the light sources 31, 32, and 33 converge to a convergence point on the back focal plane of the first lens assembly 4. Figure 7 shows that the light beam emitted by a particular light source results in a particular angle of incidence on the back focal plane of the first lens assembly 4, which may depend on the relative position between such light source and the optical axis 2. Therefore, the angle of incidence of light directed by the first lens assembly 4 on the back focal plane of the first lens assembly 4 can be customized by selecting a given light configuration of the light source assembly 3.

[0096] In the embodiment of Figure 7, a probing volume 7 is defined along the back focal plane of the first lens assembly 4. The probing volume 7 of Figure 7 receives light having three different angles of incidence. The light received by the probing volume 7 is referred to as the probing light. Because the light directed by the first lens assembly 4 forms the probing light without any optical elements downstream of the first lens assembly 4, the light directed by the first lens assembly 4 directly defines the probing light.

[0097] The characteristics of the probing light, such as the angle of incidence, can be customized by the light source assembly 3. To achieve such customization, the biological fluid analyzer 100 includes a controller 8 configured to control the light source assembly 3.

[0098] 7, the light source assembly 3 is controlled by the controller 8 according to a light configuration in which the light source assembly has a second light configuration. As explained above, the second light configuration produces light in which three convergence points on the back focal plane of the first lens assembly 4, i.e., on the probing volume 7, include light with three different angles of incidence.

[0099] 8 illustrates the biological fluid analyzer 100 of the embodiment of FIG. 7 when the controller 8 controls the light source assembly 3 according to a first light configuration. In the configuration of FIG. 8, the first light source 31 emits light, while the second light source 32 and the third light source 33 do not emit light. This configuration results in a probing light having a 0 degree angle of incidence with respect to the optical axis 2.

[0100] The controller 8 can control the light sources 31, 32, 33 of the light source assembly 3 according to a light configuration that includes light parameters other than or in addition to the relative positions of the emitting light sources with respect to the optical axis 2. For example, the controller 8 can vary the light intensity of one or more of the light sources 31, 32, 33. For example, the first light source 31 can be a blue LED, the second light source 32 can be a green LED, and the third light source 33 can be a red LED. In such an example, the second light configuration described above includes a second color scheme in which the probing light is white as a result of the combination of blue, green, and red light rays. The first light configuration described above includes a first color scheme in which the probing light is blue because, in this example, only the blue LED emits light.

[0101] Customization of the probing light, such as customization of the angle of incidence, intensity, color scheme, or combinations of these parameters of the probing light, can be useful for identifying different blood parameters in the prepared biological fluid sample, such as blood cell type classification, once a container containing the prepared blood sample is received within the probing volume 7.

[0102] The imaging system 1 of Figures 7 and 8 includes a third lens assembly 9 configured to direct light from the probing volume 7. The third lens assembly 9 focuses the light from the probing volume 7 to a convergence point on the back focal plane of the third lens assembly 9. The biological fluid analyzer 1 in Figures 7 and 8 includes a light sensor 11 that receives the light directed by the third lens assembly 9. The light sensor 11 in these figures is configured to provide a graphical representation of a blood parameter of the prepared biological fluid sample determined or estimated by the probing light when the prepared biological fluid sample is disposed in a container received within the probing volume 7. In this embodiment, the light sensor 11 is disposed in the back focal plane of the third lens assembly 9.

[0103] 7 and 8 is configured so that it is not replaceable and not directly accessible during normal operation of the biological fluid analyzer 1. Alternatively, the optical sensor 7 may be replaceable. In the embodiment of Figures 7 and 8, the probing volume 7 and the container that is likely to contain the prepared biological fluid sample are not displaced relative to the light source assembly 3 when the controller 8 sends the first and second light control signals.

[0104] It is noted that the imaging system 1 of the biological fluid analyzer 100 of the embodiment of Figures 7 and 8 may include the same aperture device 5 and second lens assembly 6 as those of the embodiment of Figures 1 and 2. As shown in these figures, the aperture device 5 and second lens assembly 6 would be disposed downstream of the first lens assembly 4 and upstream of the probing volume 7.

[0105] 9 and 10 show a light source assembly 3 comprising a first annular light source array 34 and a second annular light source array 35 that are adjacent to and concentric with each other around the optical axis 2. The second annular light source array 35 is disposed outside the first annular light source array 34, i.e., the radius of the second annular light source array 35 is greater than the radius of the first annular light source array 34. A controller 8 controls the light sources that make up the first annular light source array 34 and the second annular light source array 35.

[0106] 9, the controller 8 sends a first light control signal to the first annular light source array 34 and the second annular light source array 35. The first annular light source array 34 emits light according to a first geometric pattern having the annular shape of the first annular light source array 34, while the second annular light source array 35 does not emit light.

[0107] 10, the controller sends second light control signals to the first annular light source array 34 and the second annular light source array 35. The second annular light source array 35 emits light according to a second geometric pattern having the annular shape of the second annular light source array 35, while the first annular light source array 34 does not emit light.

[0108] 1-10 , in each of the examples described above, the probing volume 7 is optionally received by a multi-use cuvette (not shown). In such examples, the biological fluid analyzer 1 may be configured as a multi-use device including the necessary fluidic system and mechanisms (not shown) for performing multiple measurements. The biological fluid analyzer 1 of such examples may further include a solution pack containing one or more solutions (not shown) that the example biological fluid analyzer 1 is configured to administer and dispense by use of its fluidic system, and a mechanism for at least partially automatically preparing a prepared body fluid sample after aspiration, and configured to control the fluidic system to perform a washing program for at least the multi-use cuvette before and / or after biological fluid analysis.

[0109] Both the first and second geometric patterns are decentered from the optical axis 2. This can result in the probing light having an incident angle that forms a specific angle different from 0 degrees with the optical axis 2. The second geometric pattern / light pattern can result in the probing light having a larger incident angle than the first geometric pattern / light pattern.

[0110] The use of the terms "first," "second," "third," etc. is included to identify particular elements, but does not imply any particular order. Furthermore, the use of the terms "first," "second," "third," etc. is used to distinguish one element from another, but does not denote any order or importance. Note that the words "first," "second," "third," etc. are used herein and elsewhere merely for labeling purposes, and are not intended to denote any particular spatial or temporal ordering.

[0111] Furthermore, the labeling of a first element does not imply the presence of a second element, and vice versa. It should be noted that the word "a" or "an" preceding an element does not exclude the presence of a plurality of such elements.

[0112] It should further be noted that any reference signs do not limit the scope of the claims, that the disclosed examples and embodiments may be implemented at least in part by both hardware and software, and that several "systems" or "devices" may be referred to by the same item of hardware.

[0113] As used herein with reference to the present invention, the terms "upstream" and "front" and "downstream" and "rear" are used to describe the relative location of a component or a portion of a component of a biological fluid analyzer with respect to the direction in which light travels from the light source assembly to the output of the biological fluid analyzer. The biological fluid analyzer has a proximal end where the output of the biological fluid analyzer is disposed. The proximal end of the biological fluid analyzer may also be referred to as the downstream end. The light source assembly is typically located at the distal end. The distal end of the biological fluid analyzer may also be referred to as the upstream end. Components or portions of components of a biological fluid analyzer may be described as being upstream or downstream of each other based on their relative location between the proximal and distal ends of the biological fluid analyzer. A component or a portion of a component of a biological fluid analyzer is the portion of the end closest to the upstream end of the biological fluid analyzer. A component or a portion of a component of a biological fluid analyzer is the portion of the end closest to the downstream end of the biological fluid analyzer. The back focal plane of the lens assembly is the focal plane closest to the downstream end of the biological fluid analyzer. The front focal plane of the lens assembly is the focal plane closest to the upstream end of the biological fluid analyzer.

[0114] The longitudinal direction corresponds to the direction along which light substantially travels from the light source assembly to the output of the biological fluid analyzer. The longitudinal direction is typically the direction of the optical axis of the biological fluid analyzer. In other words, the term "longitudinal" refers to the direction corresponding to the main longitudinal axis of the biological fluid analyzer, which extends between the upstream and downstream ends of the biological fluid analyzer.

[0115] The term "traverse" refers to a direction perpendicular to the longitudinal axis. Any reference to a "cross-section" of a biological fluid analyzer or a component of a biological fluid analyzer refers to a transverse section unless otherwise stated.

[0116] The term "length" refers to the dimension of a component of a biological fluid analyzer in the longitudinal direction. One or more example biological fluid analyzers are described in the following clauses: Clause 1. A biological fluid analyzer comprising: 1. An imaging system, comprising: a light source assembly configured to emit light; a first lens assembly configured to direct light from the light source assembly; an aperture device configured to apply an aperture to allow light from the first lens assembly to pass through the aperture to indirectly define the probing light; Equipped with The biological fluid analyzer includes an imaging system configured to receive a probing light and defining a probing volume configured to receive a container containing a prepared biological fluid sample, and the biological fluid analyzer includes a controller configured to control an aperture device according to an aperture configuration, the aperture device configured to apply an aperture having a first aperture configuration in response to a first aperture control signal, and the aperture device configured to apply an aperture having a second aperture configuration in response to a second aperture control signal.

[0117] Clause 2. The biological fluid analyzer of clause 1, wherein the imaging system includes a second lens assembly configured to direct light from the aperture device. Clause 3. A biological fluid analyzer according to any one of clauses 1 to 2, comprising a container configured to accommodate a prepared biological fluid sample, the container being received within the roving volume.

[0118] Clause 4. The biological fluid analyzer of clause 3, wherein the container comprises a container lens assembly configured to direct light from the aperture device. Clause 5. A biological fluid analyzer described in any one of clauses 1 to 4, wherein the aperture device comprises a blade aperture, and the aperture device is configured to adjust the blade aperture as an aperture having a first aperture configuration in response to a first aperture control signal.

[0119] Clause 6. The biological fluid analyzer of clause 5, wherein the aperture device is configured to adjust the blade aperture as an aperture having a second aperture configuration in response to a second aperture control signal.

[0120] Clause 7. A biological fluid analyzer as described in any one of clauses 1 to 4, wherein the aperture device comprises a plate having a first plate aperture and a second plate aperture different from the first plate aperture, and the aperture device is configured to apply the first plate aperture as an aperture having a first aperture configuration in response to a first aperture control signal.

[0121] Clause 8. A biological fluid analyzer as described in Clause 7, wherein the aperture device includes an actuator configured to move the plate to a first position in response to a first aperture control signal, and the actuator configured to move the plate to a second position in response to a second aperture control signal.

[0122] Clause 9. A biological fluid analyzer as described in any one of clauses 7 to 8, wherein the aperture device is configured to apply the second plate aperture as an aperture having a second aperture configuration in response to a second aperture control signal.

[0123] Clause 10. A biological fluid analyzer as described in any one of clauses 1 to 4, wherein the aperture device comprises a glass substrate, and the aperture device is configured to apply a first transparency pattern in the glass substrate to provide an aperture having a first aperture configuration in response to a first aperture control signal.

[0124] Clause 11. The biological fluid analyzer of clause 10, wherein the glass substrate comprises a liquid crystal display. Clause 12. A biological fluid analyzer as described in any one of clauses 10 to 11, wherein the aperture device is configured to apply a second transparency pattern in the glass substrate in response to a second aperture control signal to provide apertures having a second aperture configuration.

[0125] Clause 13. A biological fluid analyzer according to any one of clauses 1 to 12, wherein the aperture having the first aperture configuration has a first diameter in the range of 0.5 mm to 4 mm. Clause 14. A biological fluid analyzer according to any one of clauses 1 to 13, wherein the aperture having the second aperture configuration has a second diameter in the range of 2 mm to 8 mm.

[0126] Clause 15. A biological fluid analyzer described in any one of clauses 1 to 14, wherein applying an aperture having a first aperture configuration, a second aperture configuration, or both a first aperture configuration and a second aperture configuration includes decentering the aperture from an optical axis of the imaging system.

[0127] Clause 16. A biological fluid analyzer according to any one of clauses 1 to 15, wherein one or both of the apertures having the first aperture configuration and the apertures having the second aperture configuration have an aperture shape, and the aperture shape is circular, elliptical, rectangular, or square.

[0128] Clause 17. A biological fluid analyzer according to any one of clauses 1 to 16, wherein the aperture device is configured to apply an aperture having a third aperture configuration in response to a third aperture control signal.

[0129] Clause 18. A biological fluid analyzer according to any one of clauses 1 to 17, further comprising a third lens assembly configured to direct light from the probing volume. Clause 19. The biological fluid analyzer of clause 18, further comprising a light sensor configured to receive light directed by the third lens assembly.

[0130] Clause 20. A biological fluid analyzer comprising: 1. An imaging system, comprising: a light source assembly configured to emit light; a first lens assembly configured to direct light from the light source assembly to directly or indirectly define a probing light; Equipped with the imaging system is configured to receive the probing light and defines a probing volume configured to receive a container containing the prepared biological fluid sample; Equipped with The biological fluid analyzer includes a controller configured to control a light source assembly according to a light configuration, the light source assembly configured to emit light according to a first light configuration in response to a first light control signal, and the light source assembly configured to emit light according to a second light configuration different from the first light configuration in response to a second light control signal.

[0131] Clause 21. A biological fluid analyzer as described in Clause 20, wherein the imaging system includes an aperture device configured to apply an aperture to allow light from the first lens assembly to pass through the aperture.

[0132] Clause 22. The biological fluid analyzer of clause 21, wherein the imaging system includes a second lens assembly configured to direct light from the aperture device. Clause 23. A biological fluid analyzer according to any one of clauses 20 to 22, comprising a container configured to accommodate a prepared biological fluid sample, the container being positioned within the probing volume.

[0133] Clause 24. The biological fluid analyzer of clauses 21 and 23, wherein the container comprises a container lens assembly configured to direct light from the aperture device. Clause 25. A biological fluid analyzer described in any one of clauses 20 to 24, wherein the first light configuration includes a first light pattern, and the light source assembly is configured to emit light having the first light pattern in response to a first light control signal.

[0134] Clause 26. A biological fluid analyzer as described in Clause 25, wherein the second light configuration includes a second light pattern, and the light source assembly is configured to emit light having the second light pattern in response to a second light control signal.

[0135] Clause 27. A biological fluid analyzer as described in Clause 26, wherein the light source assembly comprises an array of light sources, and in response to a first light control signal, the array of light sources is configured to emit light according to a first geometric pattern corresponding to the first light pattern, and in response to a second light control signal, the array of light sources is configured to emit light according to a second geometric pattern corresponding to the second light pattern.

[0136] Clause 28. A biological fluid analyzer described in any one of clauses 20 to 27, wherein the light source assembly comprises a glass substrate, and the light source assembly is configured to apply a first transparency pattern within the glass substrate to emit light having a first light configuration in response to a first light control signal, and the light source assembly is configured to apply a second transparency pattern within the glass substrate to emit light having a second light configuration in response to a second light control signal.

[0137] Clause 29. The biological fluid analyzer of clause 28, wherein the glass substrate comprises a liquid crystal display. Clause 30. A biological fluid analyzer described in any one of clauses 20 to 29, wherein the first light configuration includes a first color scheme and the light source assembly is configured to emit light having the first color scheme in response to a first light control signal.

[0138] Clause 31. A biological fluid analyzer described in any one of clauses 20 to 30, wherein the second light configuration includes a second color scheme and the light source assembly is configured to emit light having the second color scheme in response to a second light control signal.

[0139] Clause 32. A biological fluid analyzer described in any one of clauses 20 to 31, wherein the first light configuration includes a first intensity scheme, and the light source assembly is configured to emit light having the first intensity scheme in response to a first light control signal.

[0140] Clause 33. A biological fluid analyzer described in any one of clauses 20 to 32, wherein the second light configuration includes a second intensity scheme, and the light source assembly is configured to emit light having the second intensity scheme in response to a second light control signal.

[0141] Clause 34. A biological fluid analyzer according to any one of clauses 20 to 33, wherein the light source assembly is configured to emit light according to a third light configuration in response to a third light control signal from the controller.

[0142] Clause 35. A biological fluid analyzer described in any one of clauses 20 to 34, wherein the light source assembly is configured to emit light off-center from the optical axis of the imaging system in response to one or both of the first light control signal and the second light control signal.

[0143] Clause 36. A biological fluid analyzer according to any one of clauses 20 to 35, wherein the imaging system comprises a third lens assembly configured to direct light from the probing volume.

[0144] Clause 37. The biological fluid analyzer of clause 36, wherein the imaging system includes a light sensor configured to receive light directed by the third lens assembly. Clause 38. A biological fluid analyzer according to any one of clauses 20 to 37, which is a biological fluid analyzer according to any one of clauses 1 to 19.

[0145] Clause 39. A biological fluid analyzer comprising: 1. An imaging system, comprising: a light source assembly configured to emit light; a first lens assembly configured to direct light from the light source assembly to directly or indirectly define a probing light; Equipped with the imaging system is configured to receive the probing light and defines a probing volume configured to receive a container containing the prepared biological fluid sample; Equipped with The biological fluid analyzer includes a controller, the controller configured to control the imaging system according to the probing light configuration.

[0146] Clause 40. A biological fluid analyzer as described in Clause 39, wherein the imaging system is configured according to a first probing light configuration in response to a first probing light control signal, and the imaging system is configured according to a second probing light configuration in response to a second probing light control signal.

[0147] Clause 41. The biological fluid analyzer of clause 40, wherein the first probing light configuration, the second probing light configuration, or both the first probing light configuration and the second probing light configuration include one or more of an incident angle configuration, a light configuration, and an aperture configuration.

[0148] Clause 42. A biological fluid analyzer according to any one of clauses 39 to 41, which is a biological fluid analyzer according to clause 38. Clause 43. A biological fluid analyzer according to any one of clauses 1 to 42, which is a blood analyzer.

[0149] Clause 44. A biological fluid analyzer according to any one of clauses 1 to 42, which is a cell culture analyzer. [Explanation of symbols]

[0150] 1 Biological Fluid Analyzer 2 optical axis 3 Light Source Assembly 4 First Lens Assembly 5 Aperture Device 6 Second Lens Assembly 7 Probing Volume 8 Controller 9 Third Lens Assembly 11 Optical Sensor 31 First Light Source 32 Second Light Source 33 The Third Light Source 34 First annular light source array 35 Second annular light source array 41 first lens of first lens assembly 42 second lens of first lens assembly 43 third lens of first lens assembly 51 Blade Aperture 52 Blades 53 Plate 54 First Plate Aperture 55 Second Plate Aperture 56 Actuator 57 Glass substrate 58 yen 59, 60 Annular ring 61 first lens of second lens assembly 62 second lens of second lens assembly

Claims

1. 1. A biological fluid analyzer comprising:

1. An imaging system, comprising: a light source assembly configured to emit light; a first lens assembly configured to direct the light from the light source assembly; an aperture device configured to apply the aperture to allow light from the first lens assembly to pass through the aperture to indirectly define a probing light; and Equipped with the imaging system is configured to receive the probing light and defines a probing volume configured to receive a container containing a prepared biological fluid sample. Equipped with the biological fluid analyzer comprises a controller configured to control the aperture device according to an aperture configuration, the aperture device configured to apply apertures having a first aperture configuration in response to a first aperture control signal, and the aperture device configured to apply apertures having a second aperture configuration in response to a second aperture control signal; the first aperture configuration produces probing light having a first angle of incidence while blocking probing light having a second angle of incidence, and the second aperture configuration produces probing light having the first angle of incidence and the second angle of incidence.

2. The biological fluid analyzer of claim 1 , wherein the imaging system comprises a second lens assembly configured to direct light from the aperture device.

3. 10. The biological fluid analyzer of claim 1, comprising a container configured to hold a prepared biological fluid sample, the container received within the probing volume, the container optionally comprising a container lens assembly configured to direct light from the aperture device.

4. 2. The biological fluid analyzer of claim 1, wherein the aperture device comprises a blade aperture, and the aperture device is configured to adjust the blade aperture as an aperture having the first aperture configuration in response to the first aperture control signal.

5. 5. The biological fluid analyzer of claim 4, wherein the aperture device is configured to adjust the blade aperture as an aperture having the second aperture configuration in response to the second aperture control signal.

6. 2. The biological fluid analyzer of claim 1, wherein the aperture device comprises a plate having a first plate aperture and a second plate aperture different from the first plate aperture, the aperture device configured to apply the first plate aperture as an aperture having the first aperture configuration in response to the first aperture control signal, and the aperture device configured to apply the second plate aperture as an aperture having the second aperture configuration in response to the second aperture control signal.

7. 7. The biological fluid analyzer of claim 6, wherein the aperture device comprises an actuator configured to move the plate to a first position in response to the first aperture control signal, and the actuator configured to move the plate to a second position in response to the second aperture control signal.

8. 10. The biological fluid analyzer of claim 1, wherein the aperture device comprises a glass substrate comprising a liquid crystal display, and the aperture device is configured to apply a first transparency pattern in the glass substrate to provide apertures having the first aperture configuration in response to the first aperture control signal.

9. 9. The biological fluid analyzer of claim 8, wherein the aperture device is configured to apply a second transparency pattern in the glass substrate in response to the second aperture control signal to provide apertures having the second aperture configuration.

10. A biological fluid analyzer as described in claim 1, wherein the aperture having the first aperture configuration has a first diameter within the range of 0.5 mm to 4 mm.

11. A biological fluid analyzer as described in claim 1, wherein the aperture having the second aperture configuration has a second diameter within the range of 2 mm to 8 mm.

12. The biological fluid analyzer of claim 1, wherein applying the first aperture configuration, the second aperture configuration, or an aperture having both the first aperture configuration and the second aperture configuration includes decentering the aperture from the optical axis of the imaging system.

13. The biological fluid analyzer of claim 1, wherein one or both of the aperture having the first aperture configuration and the aperture having the second aperture configuration have an aperture shape, and the aperture shape is circular, elliptical, rectangular, or square.

14. The biological fluid analyzer of claim 1 , wherein the aperture device is configured to apply an aperture having a third aperture configuration in response to a third aperture control signal.

15. 15. The biological fluid analyzer of claim 1, further comprising a third lens assembly configured to direct light from the probing volume and a light sensor configured to receive light directed by the third lens assembly.

Images