Surface Treatment

Abstract

A method for treating a surface comprising the step of treating the surface with bacterial spores and Saccharomyces supernatant.A product comprising bacterial spores and Saccharomyces supernatant.

Description

[Technical Field]

[0001] The present invention relates to methods of treating surfaces to reduce and / or eliminate malodors during and after treatment. The present invention also relates to products that provide sustained malodor removal. [Background technology]

[0002] Cleaning and odor are closely related. The presence of malodor on a surface implies a lack of cleanliness. Malodor can be associated with a dirty surface or can occur during use on a previously cleaned surface. In the case of fabrics, malodor can occur when the fabric is damp or wet, for example, after the washing process or in humid weather. Malodor can also occur during the wear of the fabric.

[0003] WO 2020 / 150587 A1 discloses a laundry composition comprising a detergent surfactant and a Saccharomyces fermentation filtrate, which provides benefits in eliminating, inhibiting, and / or reducing any malodor, such as moisture malodor, body malodor, or food malodor.

[0004] WO 2017 / 157771 A1 discloses a process for controlling malodour using bacterial spores that can inhibit or prevent the production of malodour. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] International Publication No. 2020 / 150587(A1) [Patent Document 2] International Publication No. 2017 / 157771(A1) Summary of the Invention [Problem to be solved by the invention]

[0006] While various methods have been provided for removing, inhibiting, and / or reducing malodors, there remains a need to provide improved methods for removing, inhibiting, preventing, and / or reducing malodors from surfaces during and after the cleaning process. [Means for solving the problem]

[0007] According to a first aspect of the present invention there is provided a method of treating a surface, the method comprising: a) bacterial spores, preferably Bacillus spores; b) Saccharomyces supernatant; The method includes a processing step of treating with

[0008] According to a second aspect of the present invention there is provided a product suitable for use in the method of the present invention, said product comprising: a) bacterial spores, preferably Bacillus spores; b) Saccharomyces supernatant; Includes.

[0009] The methods and products of the present invention can be applied to any surface, and can be applied to hard or soft surfaces. The methods and products of the present invention are particularly suited for treating fabrics in the laundering process.

[0010] According to a third aspect of the present invention there is provided the use of the method of the present invention to provide sustained malodour removal from fabrics over an extended period of time.

[0011] The elements of the composition of the invention described in relation to the first aspect of the invention apply mutatis mutandis to the other aspects of the invention. DETAILED DESCRIPTION OF THE INVENTION

[0012] The present invention encompasses a method of treating a surface. The surface may be a hard or soft surface, and preferably the surface is a fabric. The method comprises treating the surface with: a) bacterial spores, preferably Bacillus spores; b) Saccharomyces supernatant; The method includes a step of treating with

[0013] The present invention also encompasses products for treating surfaces. The methods and products of the present invention provide malodor removal and prevention for a sustained period of time, not only during treatment, but also during use of the surface after it has been treated.

[0014] The methods and products of the present invention have been unexpectedly found to provide a sustained synergistic effect on malodor removal and prevention. This synergistic effect has surprisingly been found over a sustained period of time. Without being bound by theory, it is believed that the benefits of the mixed system, i.e., bacterial spores and Saccharomyces supernatant, result from the creation of a highly complex cocktail of enzymes from the combination of enzymes already present in the Saccharomyces supernatant with enzymes produced by the bacterial spores after they germinate and grow on the fabric. Because yeasts such as Saccharomyces cerevisiae are very different from bacteria, their enzymes have very different structures than those produced by bacteria. The end result is a highly efficient system that can destroy odors far more effectively than the two components individually.

[0015] The present invention also encompasses the use of the method of the present invention to provide sustained malodor removal from fabrics. By "sustained malodor removal" is meant that malodor removal occurs for at least 24 hours, preferably at least 48 hours, after the fabric has been treated. Without being bound by theory, it is believed that bacterial spores germinate with heat and sweat from the user, thereby providing malodor removal and prevention while the fabric is being worn.

[0016] As used herein, the articles "a" and "an," when used in a claim, are understood to mean one or more of what is claimed or described. As used herein, the terms "include," "includes," and "including" are meant to be open-ended. The compositions of the present disclosure may comprise, consist essentially of, or consist of the components of the present disclosure.

[0017] All percentages, ratios, and proportions used herein are by weight of the composition unless otherwise specified. All average values ​​are calculated "by weight" of the composition unless otherwise expressly indicated. All ratios are calculated as weight / weight levels unless otherwise specified.

[0018] Unless otherwise specified, all measurements are performed at 25°C.

[0019] Unless otherwise noted, all component or composition concentrations are in terms of the active portion of that component or composition and are exclusive of impurities, e.g., residual solvents or by-products, that may be present in commercial sources of such component or composition.

[0020] How to treat the surface The present disclosure relates to a method of treating a surface, which may be a hard or soft surface, preferably the surface is a soft surface, and more preferably the surface is a fabric. a) bacterial spores, preferably Bacillus spores; b) Saccharomyces supernatant; is processed.

[0021] For example, the method of the present disclosure may include contacting a fabric with a product according to the present disclosure. The contacting may occur, in whole or in part, in the presence of water. The product, or a portion thereof, may be diluted and / or dissolved in water to form a treatment solution.

[0022] The methods of the present disclosure may include contacting a surface, preferably a fabric, with an aqueous treatment solution. The aqueous treatment solution contains about 1 x 10 total bacterial spores, preferably Bacillus spores. 2 Colony forming units (CFU) ~ approx. 1 x 10 10 CFU, preferably about 1 x 10 3 C CFU ~ approx. 1 x 10 9 CFU, more preferably about 1 x 10 4 C CFU ~ approx. 1 x 10 8 The aqueous treatment liquid may contain Saccharomyces supernatant in an amount of about 0.001% by weight to about 10% by weight, preferably about 0.01% by weight to about 5% by weight of the liquid.

[0023] The method for treating fabrics can be carried out in whole or in part in any suitable container, for example, in an automatic washing machine. Such machines can be top-loading or front-loading machines. The entire process can be carried out in the washing machine. Alternatively, part of the process can be carried out in the washing machine and part of the process can be carried out in the dryer. The method of the present invention is also suitable for hand-washing applications.

[0024] The treatment step may be part of the wash or rinse cycle of an automatic washing machine. The aqueous treatment solution may be an aqueous rinse solution. The product according to the present disclosure may be added to the drawer or drum of the automatic washing machine during the wash or rinse cycle.

[0025] The treatment step of the disclosed method may include contacting fabrics with an aqueous wash liquor. The step of contacting fabrics with the aqueous wash liquor may occur before contacting the fabrics with an aqueous rinse liquor. Such a step may occur during a single treatment cycle. The aqueous wash liquor may include a cleaning composition, such as a granular or liquid laundry detergent composition, dissolved or diluted in water. The detergent composition may include an anionic surfactant. The aqueous wash liquor may include from about 50 ppm to about 5000 ppm, or from about 100 ppm to about 1000 ppm, of anionic surfactant.

[0026] The method of the present invention can include a laundry process including wash, rinse, and dry cycles, and bacterial spores and / or Saccharomyces supernatant can be applied to fabrics separately or together from a cleaning composition and / or an additive composition. The Saccharomyces supernatant can be applied to the wash cycle. The bacterial spores can be applied to the wash cycle, rinse cycle, or dry cycle. When the Saccharomyces supernatant is added to the wash cycle, it can form an aqueous solution containing about 0.01 to about 10 ppm, preferably about 0.05 to about 1 ppm, of Saccharomyces supernatant. The Saccharomyces supernatant can be applied from the cleaning composition.

[0027] Bacterial spores, preferably Bacillus spores, may be added from an additive composition at a concentration of about 0.01% to about 5% by weight of the fabric. Preferably, the bacterial spores are provided in the form of beads or from a dryer sheet. The Saccharomyces supernatant and bacterial spores may be added from the same product.

[0028] The treated fabric may be a synthetic fabric. Suitable synthetic fabrics include polyester, acrylic, nylon, rayon, acetate, spandex, lastex, and / or orlon fabrics. The process of the present invention provides excellent malodor removal and / or prevention on synthetic fabrics.

[0029] The treated fabric may comprise synthetic fibers. Suitable synthetic fibers may include polyester, acrylic, nylon, rayon, acetate, spandex, lastex, and / or Orlon fibers. The fibers may be elastic and / or contain elastane. The fabric may comprise a blend of synthetic and natural fibers (e.g., a polycotton blend). The fabric may comprise fibers that are relatively hydrophobic (e.g., compared to cotton fibers).

[0030] product The present disclosure relates to products for treating fabric surfaces, preferably fabric treatment products. As used herein, the phrase "fabric treatment product" includes compositions and formulations designed for treating fabrics, such as clothing, or other textiles.

[0031] Such products may include, but are not limited to, laundry cleaning compositions and detergents, fabric softening compositions, fabric enhancing compositions, fabric deodorizing compositions, laundry pre-cleaning agents, laundry pre-treatment agents, laundry additives, spray products, dry cleaning agents or compositions, laundry rinse additives, cleaning additives, post-rinse fabric treatment agents, ironing aids, unit dose formulations, delayed delivery formulations, detergents contained on or in porous substrates or nonwoven sheets, and other suitable forms that may be apparent to those skilled in the art in light of the teachings herein. Such products may be used as laundry pre-treatment agents, laundry post-treatment agents, or may be added during the wash and / or rinse cycles of the laundry process. Alternatively, the product or a portion thereof, particularly a composition containing bacterial spores, may be added to a dryer.

[0032] The product may be in any suitable form. The product may be in the form of a liquid composition, a granular composition, a single-compartment pouch, a multi-compartment pouch, a sheet, a lozenge or bead, a fibrous article, a tablet, a bar, a flake, or a mixture thereof. The product may be selected from a liquid, a solid, or a combination thereof.

[0033] The product may be a liquid composition. The composition may comprise about 30% to about 90%, or about 50% to about 80% water by weight of the composition. The pH of the composition may be optimized to promote bacterial spore growth and Saccharomyces supernatant growth.

[0034] The product may be a cleaning or additive composition and may be in the form of a unitized dose article, such as a tablet, pouch, sheet, or fibrous article. Such pouches typically include a water-soluble film, e.g., a polyvinyl alcohol water-soluble film, that at least partially encapsulates the composition. Suitable films are available from MonoSol, LLC (Indiana, USA). The composition may be enclosed in a single-compartment pouch or a multi-compartment pouch. A multi-compartment pouch may have at least two, at least three, or at least four compartments. A multi-compartment pouch may include compartments arranged side-by-side and / or stacked. The composition contained in the pouch or its compartments may be liquid, solid (e.g., powder), or a combination thereof. The pouch composition may have a relatively low amount of water, e.g., less than about 20%, or less than about 15%, or less than about 12%, or less than about 10%, or less than about 8% by weight of the detergent composition.

[0035] The product may be in the form of a tablet or beads. The tablet may contain polyethylene glycol as a carrier. The polyethylene glycol may have a weight average molecular weight of about 2000 to about 20,000 daltons, preferably about 5000 to about 15,000 daltons, and even more preferably about 6000 to about 12,000 daltons. Preferably, the tablet contains bacterial spores.

[0036] The product may contain a non-aqueous solvent that can act as a carrier and / or promote stability. Non-aqueous solvents may include organic solvents such as methanol, ethanol, propanol, isopropanol, 1,3-propanediol, 1,2-propanediol, ethylene glycol, glycerin, glycol ethers, hydrocarbons, or mixtures thereof. Other non-aqueous solvents may include lipophilic fluids such as siloxanes or other silicones, hydrocarbons, perfluorinated amines, perfluorinated and hydrofluoroether solvents, or mixtures thereof. Amine-containing solvents such as monoethanolamine, diethanolamine, and triethanolamine may be suitable.

[0037] Bacterial spores Although bacterial spores may be present on surfaces, the methods of the present invention involve intentionally adding bacterial spores to surfaces in an amount that can provide a significant benefit to the consumer, particularly the benefit of malodor removal and prevention. Preferably ... 2 CFU / g of surface, preferably at least 1 × 10 3 CFU / g of surface, preferably at least 1 × 10 4 CFU / g of surface, preferably at least 1 × 10 5 CFU / g, preferably 1 x 10 12 The intentional addition of less than CFU / g is required. By "intentional addition of bacterial spores" herein is meant that the spores are added in addition to any microorganisms that may be present on the fabric.

[0038] The microbial spores used in the methods and products of the present invention can be added to the wash, rinse, or dry cycle. The spores are not inactivated by heat at temperatures found in washing machines or dryers. The spores are fabric-persistent, providing malodor control during and after the laundering process, particularly during and after fabric use (e.g., wearing). Another example is found in towels, which can develop malodors after being used and left in the humid environment of a bathroom. The bacterial spores combined with Saccharomyces fermentation filtrate provide continuous malodor control.

[0039] The microbial spores of the methods and products of the present invention can germinate on fabrics. The spores can be activated by heat, such as heat generated during use of the fabric or heat provided in a washing machine or dryer. The spores can germinate when the fabric is stored and / or used. Malodor precursors can be used by the microorganisms produced by the spores as nutrients to promote germination.

[0040] Fabrics can be treated in a wet laundering process, or can be treated wet after washing in a dryer, for example, or after spraying. The washing process reduces the amount of microorganisms and metabolites on the fabric, but additional bacteria from the washing machine and wash water can be transferred to the fabric. Alternatively, the fabrics can be treated dry to refresh them.

[0041] Microbial spores for use herein are: i) capable of surviving temperatures found in a dryer; ii) fabric-persistent; iii) capable of odor control; and iv) preferably capable of supporting the cleaning action of laundry detergents. The spores have the ability to germinate and form cells during processing, and continue to germinate and form cells on the fabric using malodor precursors as nutrients. The spores can be provided in liquid or solid form. Preferably, the spores are in solid form. The spores can be provided to the drying process from a reservoir, dryer ball, solid carrier, such as a pouch, pellet, bead, tablet, dryer sheet, or the like. Preferably, the pellets are substantially spherical and / or cylindrical and have a diameter of about 1 mm to about 30 mm. Preferably, the spores are provided from a dryer sheet.

[0042] Some Gram-positive bacteria have a two-stage life cycle. During this life cycle, bacteria growing under certain conditions, such as in response to nutrient deficiency, can execute an elaborate developmental program leading to spore or endospore formation. Bacterial spores are protected by a coat composed of approximately 60 different proteins assembled into a biochemically complex structure with intriguing morphological and mechanical properties. The protein coat is considered a static structure that provides rigidity and primarily acts as a sieve to filter out large, exogenous, toxic molecules, such as lytic enzymes. Spores are highly resistant to extreme environmental conditions and therefore play an important role in the long-term survival of a species. Spores can also remain metabolically dormant for many years. Methods for obtaining bacterial spores from vegetative cells are well known in the art. In some instances, vegetative bacterial cells are grown in liquid culture. From late logarithmic or early stationary phase, bacteria can initiate spore formation. Once the bacteria have completed sporulation, the spores can be harvested from the culture medium, for example, by centrifugation. Various methods can be used to kill or remove any remaining vegetative cells. Various methods can be used to purify spores from cellular debris and / or other materials or substances. Bacterial spores can be differentiated from vegetative cells using various techniques, such as phase contrast microscopy, automated scanning microscopy, high-resolution atomic force microscopy, or thermotolerance methods. Bacterial spores are generally metabolically inactive or dormant, environmentally resistant structures, making them easily selected for use in commercial microbial products. Despite their hardiness and extremely long lifespan, spores can rapidly respond to the presence of specific small molecules known as germination, which signals favorable conditions for interrupting dormancy by germination, the initial step in the process of completing their life cycle by reverting to vegetative bacteria. For example, commercial microbial products can be designed so that spores are dispersed into an environment where they encounter germs present in the environment, germinate within vegetative cells, and perform their intended function. A variety of different bacteria can form spores. Bacteria from any of these groups can be used in the compositions, methods, and kits disclosed herein.For example, the following genera: Acetonema, Alcalibacillus, Ammoniphilus, Ampibacillus, Anaerobacter, Anaerospora, Aneuribacillus, Anoxybacillus, Bacillus, Brevibacillus, Cardanaerobacter, Caloramater, Caminicella, Serrasibacillus, Clostridium, Clostridium disalibacter, Cornella, Dendrosporobacter, Desulfotomaculum, Desulfosporomusa, Desulfosporosinus S., Desulfovirgra, Desulfnispora, Desulfrispora, Filifactor, Filobacillus, Gerria, Geobacillus, Geosporobacter, Gracilibacillus, Halonatronum, Heliobacterium, Heliophyllum, Raceella, Lentinibacillus, Raisinibacillus, Mahela, Metabacterium, Moorella, Natroniella, Oceanobacillus, Olenia, Ornithinebacillus, Oxalophagus, Oxobacillus Tar, Paenibacillus, Paraliobacillus, Perospora, Perotomaculum, Piscibacillus, Planiphyllum, Pontibacillus, Propionispora, Salinibacillus, Sarsuginibacillus, Seinonella, Simazuela, Sporacetigenium, Sporoanaerobacter, Sporobacter, Sporobacterium, Sporohalobacter, Sporolactobacillus, Sporomusa, Sporosalcia, Sporotalea, Sporotomaculum, Si Some bacteria among the following can form spores: Ntrophomonas, Syntrophospora, Tenuibacillus, Tepidibacter, Teribacillus, Thalassobacillus, Thermoacetogenium, Thermoactinomyces, Thermoalkalibacillus, Thermoanaerobacter, Thermoanaeromonas, Thermobacillus, Thermoflavimicrobium, Thermovenablum, Tuberibacillus, Bulgibacillus, and / or Vulcanobacillus.

[0043] Preferably, the bacterium capable of forming spores is a bacterium of the Bacillaceae family, for example, Aeribacillus, Allibacillus, Alkalibacillus, Alkalicoccus, Alkalihalobacillus, Alkalicactibacillus, Allobacillus, Arteribacillus, Arteribacter, Ampibacillus, Anaerobacillus, Anoxybacillus, Aquibacillus, Aquisaribacillus, Aureibacillus, Bacillus, Caldarcalibacillus, Caldibacillus, Calditericola, Caldifontisbacillus, Cameliibacillus, Serrasibacillus, Compostibacillus, Rus, Cytobacillus, Desertibacillus, Domibacillus, Ectobacillus, Evansella, Farcibacillus, Ferdinandcohina, Fermentibacillus, Fictibacillus, Filbacillus, Geobacillus, Geomicrobium, Gottfriedia, Gracilibacillus, Hallalkalibacillus, Halobacillus, Haloractibacillus, Heindrixia, Hydrogenibacillus, Lederbergia, Lentibacillus, Richfieldia, Rottidebacillus, Margaritia, Marinococcus, Mergilibacillus, Mesobacillus, Metabacillus, Microaerobacter, Natoribacillus, Natronobacillus, Neobacillus, Niaria, Oceanobacillus, Ornithinibacillus, Parageobacillus, Paraliobacillus, Paralcalibacillus, Paucisalibacillus, Pelagirhabdos, Peribacillus, Piscibacillus, Polygonibacillus, Pontibacillus, Pradosia, Prieszia, Pseudogracilibacillus, Pueribacillus, Radiobacillus, Robertomuraya, Rosellomorea, Saccharococcus, Salibacterium, Salimicrobium, In various examples, the bacteria are from species of the genera Salinibacillus, Salipaldibacillus, Salirhabdus, Salisediminibacterium, Saliteribacillus, Salsiuginibacillus, Sediminibacillus, Siminovichia, Sinibacillus, Sinobacillus, Streptohalobacillus, Sacrifiella, Swionibacillus, Tenuibacillus, Tepidibacillus, Terribacillus, Terrilacticbacillus, Texcoconibacillus, Thalassobacillus, Thalassorhabdus, Thermolongibacillus, Bardibacillus, Bardibacillus, Vulcanibacillus, and Weizmania.Bacillus aerophilus, Bacillus albus, Bacillus alticuzinis, Bacillus albeauensis, Bacillus amyloliquefaciensex, Bacillus anthracis, Bacillus aquiflavi, Bacillus atrophaeus, Bacillus australimalis, Bacillus badius, Bacillus benzoevorans, Bacillus cabriaresii, Bacillus canaverarius, Bacillus cappallidis, Bacillus carboniphilus, Bacillus cereus, Bacillus chagangensis, Bacillus corefirense, Bacillus cytotoxicus, Bacillus decisifrondis, Bacillus ectoiniformans, Bacillus enculensis, Bacillus fengquensis, Bacillus fungorum, Bacillus glitinifermentans, Bacillus gobiensis, Bacillus halotolerans, Bacillus heineshii, Bacillus forti, Bacillus inaquosorum, Bacillus infantis, Bacillus infernus, Bacillus isaberiae, Bacillus quequeae, Bacillus licheniformis, Bacillus luti, Bacillus manusensis, Bacillus ma Bacillus linisedimentorum, Bacillus mesophilus, Bacillus methanolicus, Bacillus mobilis, Bacillus mojavensis, Bacillus mycoides, Bacillus nakamurai, Bacillus nudgiopicus, Bacillus nitrachiredusens, Bacillus oleivorans, Bacillus pacificus, Bacillus pachystanensis, Bacillus paralicheniformis, Bacillus paramycoides, Bacillus paranthrasis, Bacillus pervagus, Bacillus pisticola, Bacillus proteolyticus, Bacillus pseudomycoides, Bacillus proteolyticus Millis, Bacillus safensis, Bacillus tharracetis, Bacillus salinus, Bacillus salitorrans, Bacillus theohaeanensis, Bacillus sibazii, Bacillus siamensis, Bacillus smithii, Bacillus solimanglobi, Bacillus songkurensis, Bacillus sonorensis, Bacillus spizizenii, Bacillus spongiae, Bacillus stearicolis, Bacillus stratosphericus, Bacillus subtilis, Bacillus swedzei, Bacillus thaeanensis, Bacillus tamaricis, Bacillus tequilensis,The strain may be a strain of Bacillus thermocloacae, Bacillus thermotolerans, Bacillus thuringiensis, Bacillus tianchenii, Bacillus toyonensis, Bacillus tropicalis, Bacillus valismortis, Bacillus verrezuensis, Bacillus viedmannii, Bacillus vdaliankiensis, Bacillus kiamenensis, Bacillus kiapuensis, Bacillus zangzouensis, or a combination thereof.

[0044] In some examples, the spore-forming bacterial strain may be a Bacillus strain, such as Bacillus sp. strain SD-6991, Bacillus sp. strain SD-6992, Bacillus sp. strain NRRL B-50606, Bacillus sp. strain NRRL B-50887, Bacillus pumilus strain NRRL B-50016, Bacillus amyloliquefaciens strain NRRL B-50017, Bacillus amyloliquefaciens strain PTA-7792 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain NRRL B-50018, Bacillus amyloliquefaciens strain NRRL B-50019, Bacillus amyloliquefaciens strain NRRL B-50020, Bacillus amyloliquefaciens strain NRRL B-50021, Bacillus amyloliquefaciens strain NRRL B-50022, Bacillus amyloliquefaciens strain NRRL B-50023, Bacillus amyloliquefaciens strain NRRL B-50024, Bacillus amyloliquefaciens strain NRRL B-50025, Bacillus amyloliquefaciens strain NRRL B-50026, Bacillus amyloliquefaciens strain NRRL B-50027, Bacillus amyloliquefaciens strain NRRL B-50028, Bacillus amyloliquefaciens strain NRRL B-50029, Bacillus amyloliquefaciens strain NRRL B-50029, Bacillus amyloliquefaciens strain NRRL B-5 B-50018, Bacillus amyloliquefaciens strain PTA-7541, Bacillus amyloliquefaciens strain PTA-7544, Bacillus amyloliquefaciens strain PTA-7545, Bacillus amyloliquefaciens strain PTA-7546, Bacillus subtilis strain PTA-7547, Bacillus amyloliquefaciens strain PTA-7549, Bacillus amyloliquefaciens strain PTA-7793, Bacillus amyloliquefaciens strain PTA-7790, Bacillus amyloliquefaciens strain PTA-7791, Bacillus subtilis strain NRRL B-50136 (also known as DA-33R, ATCC accession number 55406), Bacillus amyloliquefaciens strain NRRL B-50141, Bacillus amyloliquefaciens strain NRRL B-50399, Bacillus licheniformis strain NRRL B-50014, Bacillus licheniformis strain NRRL B-50015, Bacillus amyloliquefaciens strain NRRL B-50607, Bacillus subtilis strain NRRL B-50147 (also known as 300R), Bacillus amyloliquefaciens strain NRRL B-50150, Bacillus amyloliquefaciens strain NRRL B-50154, Bacillus megaterium PTA-3142, Bacillus amyloliquefaciens strain ATCC accession number 55405 (also known as 300), Bacillus amyloliquefaciens strain ATCC accession number 55407 (also known as PMX), Bacillus pumilus NRRL B-50398 (ATCCBacillus cereus ATCC accession number 700386, Bacillus thuringiensis ATCC accession number 700387 (all of the above strains are available from Novozymes, Inc., USA), Bacillus amyloliquefaciens FZB24 (e.g., isolates NRRL B-50304 and NRRL B-50349 TAEGRO® available from Novozymes), Bacillus subtilis (e.g., isolate NRRL B-21661 in RHAPSODY®, SERENADE® MAX, and SERENADE® ASO available from Bayer CropScience), Bacillus pumilus (e.g., isolate NRRL B-21661 in RHAPSODY®, SERENADE® MAX, and SERENADE® ASO available from Bayer CropScience), ...pumilus (e.g., isolate NRRL B-21661 in RHAPSODY®, SERENADE® B-50349 isolate), Bacillus amyloliquefaciens TrigoCor (also known as "TrigoCor 1448", e.g., isolate Embrapa Trigo accession number 144 / 88.4Lev, Cornell accession number Pma007BR-97, and ATCC accession number 202152 available from Cornell University, USA), and combinations thereof.

[0045] In some examples, the spore-forming bacterial strain can be a Bacillus amyloliquefaciens strain. For example, the strain can be Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus) and / or Bacillus amyloliquefaciens strain NRRL B-50154, Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain NRRL B-50154, or from other Bacillus amyloliquefaciens microorganisms.

[0046] In some examples, the spore-forming bacterial strain may be a Brevibacillus species, such as Brevibacillus brevis, Brevibacillus formosus, Brevibacillus laterosporus, or Brevibacillus parabrevis, or a combination thereof.

[0047] In some examples, the spore-forming bacterial strain may be a Paenibacillus species, such as Paenibacillus alvei, Paenibacillus amylolyticus, Paenibacillus azotofixans, Paenibacillus cookii, Paenibacillus macerans, Paenibacillus polymyxa, or Paenibacillus validus, or a combination thereof. The bacterial spores may have an average particle size of about 2 to 50 microns, preferably about 10 to 45 microns. Bacillus spores are commercially available in blends in aqueous carriers in which they are insoluble. Other commercially available bacillus spore blends include, but are not limited to, Fenshen Free™ CAN (10X), available from Novozymes Biologicals, Inc., Evogen® Renew Plus (10X), available from Genesis Biosciences, Inc., and Evogen® GT (10X, 20X, and 110X), all available from Genesis Biosciences, Inc. In the foregoing list, the designations in parentheses (10X, 20X, and 110X) indicate the relative concentrations of bacillus spores.

[0048] The bacterial spores used in the compositions, methods, and products disclosed herein may or may not be heat activated. In some instances, the bacterial spores are heat activated. In some instances, the bacterial spores are not heat inactivated. Preferably, the spores used herein are heat activated. Heat activation may involve heating the bacterial spores from room temperature (15-25°C) to an optimum temperature of 25-120°C, preferably 40-100°C, and holding the optimum temperature for up to 2 hours, preferably 70-80°C for 30 minutes.

[0049] For the methods, compositions, and products disclosed herein, a population of bacterial spores is generally used. In some examples, the population of bacterial spores may include bacterial spores from a single strain of bacteria. Preferably, the population of bacterial spores may include bacterial spores from two, three, four, five, or more strains of bacteria. Generally, the population of bacterial spores contains a majority of spores and a small number of vegetative cells. In some examples, the population of bacterial spores does not contain vegetative cells. In some examples, the population of bacterial spores may contain less than about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 40%, or 50% vegetative cells, where the percentage of bacterial spores is calculated as ((number of vegetative cells / (number of spores in the population + number of vegetative cells in the population)) × 100). Generally, the populations of bacterial spores used in the disclosed methods, compositions, and products are stable (i.e., not germinating) and at least some individual spores in the population are capable of germination.

[0050] The population of bacterial spores used in the present disclosure may contain bacterial spores at different concentrations. In various examples, the population of bacterial spores is at least 1 x 10 2 , 5×10 2 , 1×10 3 , 5×10 3 , 1×10 4 , 5×10 4 , 1×10 5 , 5×10 5 , 1×10 6 , 5×10 6 , 1×10 7 , 5×10 7, 1×10 8 , 5×10 8 , 1×10 9 , 5×10 9 , 1×10 10 , 5×10 10 , 1×10 11 , 5×10 11 , 1×10 12 , 5×10 12 , 1×10 13 , 5×10 13 , 1×10 14 , or 5 x 10 14 particles / mL, particles / gram, or particles / cm 3 The spores may include, but are not limited to, spores of Bacillus subtilis.

[0051] Dryer sheets can be conveniently used to treat fabrics during the drying process in a dryer. Dryer sheets can be used to treat unwashed fabrics or fabrics after they have been washed with laundry detergent.

[0052] Preferably, the bacterial spores comprise Bacillus spores, more preferably Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus tequilensis, Bacillus vallismortis, Bacillus mojavensis, Bacillus mojavensis), and mixtures thereof, and more preferably, the bacillus is selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, and mixtures thereof.

[0053] Saccharomyces supernatant Saccharomyces supernatant is a bacterial enzyme-containing material obtained by fermentation and filtration of Saccharomyces yeast. The supernatant can be obtained by any separation process, including filtration, dialysis, and centrifugation. Bacterial enzymes can utilize inorganic nitrogen, for example, from ammonia, to form amino acids. Saccharomyces supernatant is an environmentally friendly compound because it can be derived from organic sources, such as plant materials. Suitable Saccharomyces supernatants are supplied by Carrubba Incorporated, Milford, CT, USA. These include DeoPlex® DH (Saccharomyces fermentation filtrate), DeoPlex® Organic, and DeoPlex® Clear.

[0054] The methods and products of the present disclosure include Saccharomyces supernatant for the purpose of removing, inhibiting, and / or reducing malodors on surfaces, preferably fabrics, for example, for a period of time such as from about 1 hour to about 72 hours or more, for the purpose of inhibiting and / or reducing the development of malodors on washed wet fabrics, or for example, for the purpose of removing and / or reducing malodors resulting from exposing fabrics to a malodor source prior to washing.

[0055] The Saccharomyces supernatant may be provided as part of a cleaning composition or as part of an additive composition. Preferably, the composition is a laundry cleaning composition. The composition comprises the Saccharomyces supernatant in an amount of about 0.05 to about 10% by weight of the composition, such as about 0.05 to about 5% by weight, for example, about 0.05 to about 2% by weight, for example, about 0.05 to about 1% by weight, or about 0.05 to about 0.7% by weight.

[0056] Cleaning Composition Ingredients Suitable cleaning ingredients include at least one of a surfactant, an enzyme, an enzyme stabilizing system, a detergent builder, a chelating agent, a complexing agent, a clay soil removal / anti-redeposition agent, a polymeric soil release agent, a polymeric dispersing agent, a polymeric grease cleaner, a dye transfer inhibitor, a bleaching agent, a bleach activator, a bleach catalyst, a fabric conditioner, a clay, a suds booster, a defoamer, a suds suppressor, a corrosion inhibitor, a soil suspending agent, a dye, a hueing dye, a disinfectant, an anti-haze agent, an optical brightener, a fragrance, a saturated or unsaturated fatty acid, a calcium cation, a magnesium cation, a visual signal component, a structurant, a thickener, an anti-caking agent, a starch, a sand, a gelling agent, or any combination thereof.

[0057] Surfactant System: The composition may comprise a surfactant system in an amount sufficient to impart the desired cleaning characteristics. In some embodiments, the composition comprises from about 1% to about 70% surfactant system, by weight of the composition. In other embodiments, the liquid composition comprises from about 2% to about 60% surfactant system, by weight of the composition. In further embodiments, the composition comprises from about 5% to about 30% surfactant system, by weight of the composition. The surfactant system may comprise a detersive surfactant selected from anionic surfactants, nonionic surfactants, cationic surfactants, zwitterionic surfactants, amphoteric surfactants, ampholytic surfactants, and mixtures thereof. One skilled in the art will recognize that a detersive surfactant includes any surfactant or mixture of surfactants that provides cleaning, stain removal, or laundering benefits to soiled materials.

[0058] Anionic Surfactants. Non-limiting examples of suitable anionic surfactants include any conventional anionic surfactant, including linear alkylbenzene sulfonates (LAS), α-olefin sulfonates (AOS), alkyl sulfates (fatty alcohol sulfates) (AS), alcohol ethoxy sulfates (AEOS or AES), secondary alkane sulfonates (SAS), α-sulfofatty acid methyl esters, alkyl- or alkenyl succinic acids, or soaps.

[0059] Suitable alkylbenzene sulfonates (LAS) can be obtained by sulfonating commercially available linear alkylbenzenes (LABs). Suitable LABs include low 2-phenyl LABs, such as those supplied by Sasol under the trade name Isochem® or Petrelab® by Petresa; other suitable LABs include high 2-phenyl LABs, such as those supplied by Sasol under the trade name Hyblene®. Suitable anionic detersive surfactants are alkylbenzene sulfonates obtained by the DETAL catalyzed process, although other synthetic routes, such as HF, may also be suitable. In one embodiment, magnesium salts of LAS are used.

[0060] The detersive surfactant may be a mid-chain branched detersive surfactant, in one aspect a mid-chain branched anionic detersive surfactant, in one aspect a mid-chain branched alkyl sulfate and / or a mid-chain branched alkyl benzene sulfonate, e.g., a mid-chain branched alkyl sulfate. In one aspect, the mid-chain branched alkyl 1~4 The alkyl groups are typically methyl and / or ethyl groups.

[0061] Other anionic surfactants useful herein include the water-soluble salts of paraffin sulfonates and secondary alkane sulfonates containing from about 8 to about 24 (and in some instances, from about 12 to 18) carbon atoms; alkyl glyceryl ether sulfonates, especially C 8~18 Ethers of alcohols (e.g., derived from tallow and coconut oil). Mixtures of alkyl benzene sulfonates with the above-mentioned paraffin sulfonates, secondary alkane sulfonates, and alkyl glyceryl ether sulfonates are also useful. Further suitable anionic surfactants include methyl ester sulfonates and alkyl ether carboxylates (AECs).

[0062] Suitable anionic surfactants also include branched anionic surfactants selected from branched sulfate or branched sulfonate surfactants. Further suitable branched anionic detersive surfactants include surfactants derived from alcohols branched at the 2-alkyl position, such as those sold under the trade names Isalchem® 123, Isalchem® 125, Isalchem® 145, and Isalchem® 167, which are derived from the oxo process. Due to the oxo process, the branch is located at the 2-alkyl position. These 2-alkyl branched alcohols typically range in length from C11 to C14 / C15, and include all structural isomers branched at the 2-alkyl position.

[0063] Anionic surfactants may exist in acid form, and the acid form may be neutralized to form surfactant salts. Typical neutralizing agents include hydroxides, such as metal counterion bases, such as NaOH or KOH. Further suitable neutralizing agents for neutralizing these acid forms of anionic surfactants include ammonia, amines, or alkanolamines. Non-limiting examples of alkanolamines include monoethanolamine, diethanolamine, triethanolamine, and other linear or branched alkanolamines known in the art. Suitable alkanolamines include 2-amino-1-propanol, 1-aminopropanol, monoisopropanolamine, or 1-amino-3-propanol. Amine neutralization may be complete or partial; for example, a portion of the anionic surfactant mixture may be neutralized with sodium or potassium, and a portion of the anionic surfactant mixture may be neutralized with amines or alkanolamines.

[0064] Nonionic surfactants. Suitable nonionic surfactants useful herein can include any conventional nonionic surfactant. These can include, for example, alkoxylated fatty alcohols and amine oxide surfactants. Other non-limiting examples of nonionic surfactants useful herein include C8-C 18 Alkyl ethoxylates (NEODOL® nonionic surfactants (Shell), etc.); C6-C 12 Alkylphenol alkoxylate (the alkoxylate units can be ethyleneoxy units, propyleneoxy units, or combinations thereof); C 12 ~C 18 C6-C with alcohol and ethylene oxide / propylene oxide block polymer 12 Alkylphenol condensates (such as Pluronic® (BASF)); C 14 ~C 22 Medium-chain branched alcohol (BA); C 14 ~C 22 Medium-chain branched MEA (BAEx ), where x is 1 to 30); alkyl polysaccharides; specifically alkyl polyglycosides; polyhydroxy fatty acid amides; and ether-terminated poly(oxyalkylated) alcohol surfactants. Suitable nonionic detersive surfactants also include alkyl polyglucosides and alkyl alkoxylated alcohols. Suitable nonionic surfactants also include those sold by BASF under the trade name Lutensol®.

[0065] Cationic Surfactants. The surfactant system may include a cationic surfactant. In some embodiments, the surfactant system includes from about 0% to about 7%, from about 0.1% to about 5%, or from about 1% to about 4% by weight of the surfactant system of a cationic surfactant, e.g., as a co-surfactant. In some embodiments, the compositions of the present invention are substantially free of cationic surfactants and surfactants that become cationic at a pH below 7 or below 6. Non-limiting examples of cationic surfactants include quaternary ammonium surfactants, which may have 26 or fewer carbon atoms, including alkoxylate quaternary ammonium (AQA) surfactants; dimethylhydroxyethyl quaternary ammonium; dimethylhydroxyethyl lauryl ammonium chloride; polyamine cationic surfactants; cationic ester surfactants; and amino surfactants, such as amidopropyldimethylamine (APA). Suitable cationic detersive surfactants also include alkyl pyridinium compounds, alkyl quaternary ammonium compounds, alkyl quaternary phosphonium compounds, alkyl tertiary sulfonium compounds, and mixtures thereof.

[0066] Zwitterionic surfactants. Examples of zwitterionic surfactants include secondary and tertiary amine derivatives, heterocyclic secondary and tertiary amine derivatives, or derivatives of quaternary ammonium compounds, quaternary phosphonium compounds, or tertiary sulfonium compounds. Betaines, including alkyl dimethyl betaines and cocodimethylamidopropyl betaines, C8-C 18 (For example, C 12 ~C 18) amine oxides, and sulfo- and hydroxybetaines such as N-alkyl-N,N-dimethylamino-1-propanesulfonates (wherein the alkyl group is C8-C 18 In particular embodiments, C 10 ~C 14 (It can be said that).

[0067] Examples of amphoteric surfactants include aliphatic derivatives of secondary or tertiary amines, and heterocyclic aliphatic derivatives of secondary and tertiary amines, in which the aliphatic group may be straight or branched, and one of the aliphatic substituents contains at least about 8 carbon atoms, typically about 8 to about 18 carbon atoms, and at least one of the aliphatic substituents contains an anionic water-solubilizing group, such as carboxy, sulfonate, or sulfate. Examples of compounds falling within this definition are sodium 3-(dodecylamino)propionate, sodium 3-(dodecylamino)propane-1-sulfonate, sodium 2-(dodecylamino)ethyl sulfate, sodium 2-(dimethylamino)octadecanoate, disodium 3-(N-carboxymethyldodecylamino)propane-1-sulfonate, disodium octadecyl-iminodiacetate, sodium 1-carboxymethyl-2-undecylimidazole, and sodium N,N-bis(2-hydroxyethyl)-2-sulfato-3-dodecoxypropylamine. Suitable amphoteric surfactants also include sarcosinates, glycinates, taurinates, and mixtures thereof.

[0068] Enzymes. Preferably, the composition comprises one or more enzymes. Preferred enzymes provide cleaning performance and / or fabric care benefits. Examples of suitable enzymes include, but are not limited to, hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, mannanases, galactanases, pectate lyases, keratinases, reductases, oxidases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, malanases, β-glucanases, arabinosidases, hyaluronidases, chondroitinases, laccases, and amylases, or mixtures thereof. A typical combination is an enzyme cocktail, which may include, for example, proteases and lipases together with amylases.

[0069] Protease. Preferably, the composition comprises one or more proteases. Suitable proteases include metalloproteases and serine proteases, including, for example, neutral or alkaline microbial serine proteases such as subtilisin (EC 3.4.21.62). Suitable proteases include those of animal, plant, or microbial origin. In one aspect, such suitable proteases may be of microbial origin. Suitable proteases include chemically or genetically modified variants of the aforementioned suitable proteases. In one aspect, suitable proteases may be serine proteases, such as alkaline microbial proteases and / or trypsin-type proteases. Examples of suitable neutral or alkaline proteases include: (a) Subtilisin (EC3.4.21.62), in particular WO 2004067737, WO 2015091989, WO 2015091990, WO 2015024739, WO 2015143360, U.S. Pat. Nos. 6,312,936 (B1), 5,679,630, 4,760,025, German Patent Publication Nos. 102006022216 (A1), 102006022224 (A1), WO 2015089447, WO 2015089441, WO 2016066756 ... Those derived from Bacillus species such as B. lentus, B. alkalophilus, B. subtilis, B. amyloliquefaciens, B. pumilus, B. gibsonii, and B. akibaii, as described in Patent Nos. 016066757, 2016069557, 2016069563, and 2016069569. (b) Trypsin- or chymotrypsin-type proteases, such as trypsin (e.g., of porcine or bovine origin), including the Fusarium protease described in WO 89 / 06270 and the chymotrypsin protease derived from Cellulomonas described in WO 05 / 052161 and WO 05 / 052146. (c) Metalloproteases, particularly those derived from Bacillus amyloliquefaciens, as described in WO 07 / 044993(A2). those derived from Bacillus, Brevibacillus, Thermoactinomyces, Geobacillus, Paenibacillus, Lysinibacillus or Streptomyces spp. as described in WO 2014194032, WO 2014194054 and WO 2014194117, Kribella alluminosa as described in WO 2015193488, and Streptomyces and Lysobacter as described in WO 2016075078; (d) Proteases having at least 90% identity to the subtilase from Bacillus sp. TY145, NCIMB 40339, described in WO 92 / 17577 (Novozymes A / S), including variants of this Bacillus sp. TY145 subtilase described in WO 2015024739 and WO 2016066757.

[0070] Suitable commercially available protease enzymes include those sold under the trade names Alcalase®, Savinase®, Primase®, Durazym®, Polarzyme®, Kannase®, Liquanase®, Liquase Ultra®, Savinase Ultra®, Ovozyme®, Neutrase®, Everlase® and Esperase® by Novozymes A / S (Denmark); those sold under the trade names Maxatase®, Maxacal®, Maxapem®, Properase®, Purafect®, Purafect Prime®, Purafect Ox®, FN3®, FN4®, Excellase® and Purafect OXP® by DuPont; and those sold under the trade names Opticlean® and Optimase® by Solvay. those sold by Enzymes, available from Henkel / Kemira, namely BLAP (sequence shown in Figure 29 of U.S. Patent No. 5,352,604), and KAP available from Kao (Bacillus alkalophilus subtilisin with mutations A230V+S256G+S259N).

[0071] Amylase. Preferably, the composition may comprise an amylase. Suitable α-amylases include those of bacterial or fungal origin, including chemically or genetically modified variants. Preferred alkaline α-amylases are those derived from Bacillus species, such as Bacillus licheniformis (B. licheniformis), Bacillus amyloliquefaciens (B. amyloliquefaciens), Bacillus stearothermophilus, Bacillus subtilis, or other Bacillus species, such as Bacillus species NCIB 12289, NCIB 12512, NCIB 12513, DSM 9375 (U.S. Pat. No. 7,153,818), DSM 12368, DSM Z12649, KSM AP1378 (WO 97 / 00324), KSM K36, or KSM K38 (EP 1,022,334). (a) Variants described in WO 94 / 02597, WO 94 / 18314, WO 96 / 23874 and WO 97 / 43424, in particular variants in which one or more of the following positions have been substituted relative to the enzyme listed as SEQ ID NO:2 in WO 96 / 23874: 15, 23, 105, 106, 124, 128, 133, 154, 156, 181, 188, 190, 197, 202, 208, 209, 243, 264, 304, 305, 391, 408 and 444. (b) Variants described in U.S. Pat. No. 5,856,164 and WO 99 / 23211, WO 96 / 23873, WO 00 / 60060 and WO 06 / 002643, in particular the AA560 enzyme listed as SEQ ID NO: 12 in WO 06 / 002643, at the following positions: 26, 30, 33, 82, 37, 106, 118, 128, 133, 149, 150, 160, 178, 182, 186, 193, 203, 214, 231, 256, 257, 258, 269, 270, 272, 283, 295, 296, 298, 299, 303, 304, 305, 311, 314, 315, 318, 319, 339, 345, 361, 378, 383, 419, 421, 437, 441, 444, 445, 446, 447, 450, 461, 471, 482, 484, preferably D183 * and G184 * Mutants that also contain a deletion of (c) SEQ ID NO: 4 in WO 06 / 002643, variants exhibiting at least 90% identity to the wild-type enzyme from Bacillus sp. SP722, in particular variants with deletions at positions 183 and 184, and variants described in WO 00 / 60060, which are incorporated herein by reference. (d) Variants exhibiting at least 95% identity to the wild-type enzyme of Bacillus sp. 707 (SEQ ID NO: 7 of U.S. Pat. No. 6,093,562), particularly those containing one or more of the following mutations: M202, M208, S255, R172, and / or M261. Preferably, the amylase contains one or more of the following mutations: M202L, M202V, M202S, M202T, M202I, M202Q, M202W, S255N, and / or R172Q. Particularly preferred are those containing the M202L or M202T mutations. (e) a variant described in WO 09 / 149130, preferably one showing at least 90% identity to SEQ ID NO: 1 or SEQ ID NO: 2 in WO 09 / 149130, a wild-type enzyme derived from Geobacillus stearofermophilus or a truncated version thereof. (f) Variants exhibiting at least 89% identity to SEQ ID NO: 1 in WO2016091688, in particular those containing a deletion at positions H183+G184 and further containing one or more mutations at positions 405, 421, 422, and / or 428. (g) a variant exhibiting at least 60% amino acid sequence identity with "PcuAmyl α-amylase" derived from Paenibacillus curdlanolyticus YK9 (SEQ ID NO: 3 of WO 2014099523); (h) a variant exhibiting at least 60% amino acid sequence identity with "CspAmy2 amylase" derived from Cytophaga sp. (SEQ ID NO: 1 in WO 2014164777); (i) a variant exhibiting at least 85% identity with AmyE from Bacillus subtilis (SEQ ID NO: 1 of WO 2009149271); (j) A variant exhibiting at least 90% identity with the wild-type amylase derived from Bacillus sp. KSM-K38 under accession number AB051102.

[0072] Suitable commercially available α-amylases include DURAMYL®, LIQUEZYME®, TERMAMYL®, TERMAMYL ULTRA®, NATALASE®, SUPRAMYL®, STAINZYME®, STAINZYME PLUS®, FUNGAMYL®, and BAN® (Novozymes A / S, Bagsvaerd, Denmark), KEMZYM® AT 9000 (Biozym Biotech Trading GmbH, Wehlistrasse 27b A-1200 Wien, Austria), RAPIDASE®, PURASTAR®, ENZYSIZE®, OPTISIZE HT PLUS®, POWERASE®, and PURASTAR OXAM® (Genencor International Inc., Palo Alto, CA). Alto, California), and KAM® (Kao, 14-10 Nihonbashi Kayabacho, 1-chome, Chuo-ku, Tokyo 103-8210, Japan). In one aspect, suitable amylases include NATALASE®, STAINZYME®, and STAINZYME PLUS®, and mixtures thereof.

[0073] Lipase. Preferably, the composition comprises one or more lipases, including "first cycle lipases," such as those described in U.S. Patent No. 6,939,702 (B1) and U.S. Patent Application Publication No. 2009 / 0217464. Preferred lipases are first wash lipases. The composition may comprise a first wash lipase.

[0074] First wash lipases include lipases that are polypeptides having the following amino acid sequences: (a) Humicola lanuginosa lipase; (b) have at least 90% identity with wild-type lipase derived from (A. lanuginosa) strain DSM4109; (b) contain, compared to the wild-type lipase, substitutions of electrically neutral or negatively charged amino acids with positively charged amino acids on the surface of the three-dimensional structure within 15 A of E1 or Q249; and (c) contain a peptide addition at the C-terminus, and / or (d) contain a peptide addition at the N-terminus, and / or (e) satisfy the following restrictions: i) contain a negative amino acid at position E210 of the wild-type lipase; (ii) contain a negatively charged amino acid in the region corresponding to positions 90-101 of the wild-type lipase; and (iii) contain a neutral or electronegative amino acid at N94 or the position corresponding to the wild-type lipase and / or have a negative or neutral net charge in the region corresponding to positions 90-101 of the wild-type lipase.

[0075] Preferred are variants of wild-type lipase from Thermomyces lanuginosus, including one or more of the T231R and N233R mutations. The wild-type sequence is Swiss-Prot accession number 059952 (269 amino acids (amino acids 23 to 291) from Thermomyces lanuginosus (Humicola lanuginosa)). Preferred lipases include those sold under the trade names Lipex®, Lipolex®, and Lipoclean®.

[0076] Cellulases. Suitable cellulases are those of bacterial or fungal origin. These include chemically modified or genetically engineered variants of the protein. Suitable cellulases include cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, and Acremonium, such as fungal cellulases produced by Humicola insolens, Myceliophthora thermophila, and Fusarium oxysporum, as disclosed in U.S. Patent Nos. 4,435,307, 5,648,263, 5,691,178, 5,776,757, and 5,691,178. Suitable cellulases include alkaline or neutral cellulases with color care benefits. Commercially available cellulases include CELLUZYME®, CAREZYME®, and CAREZYME PREMIUM (Novozymes A / S), CLAZINASE®, and PURADAX HA® (Genencor International Inc.), and KAC-500® (Kao Corporation).

[0077] The bacterial cleaning cellulase may be a glycosyl hydrolase having enzymatic activity on amorphous cellulose substrates, wherein the glycosyl hydrolase is selected from GH family 5, 7, 12, 16, 44, or 74. Suitable glycosyl hydrolases may also be selected from GH family 44 glycosyl hydrolases (wild type) from Paenibacillus polyxyma, such as XYG1006, as described in U.S. Pat. No. 7,361,736, or variants thereof. GH family 12 glycosyl hydrolases (wild-type) from Bacillus licheniformis, such as SEQ ID NO: 1 as described in U.S. Pat. No. 6,268,197, or variants thereof; GH family 5 glycosyl hydrolases (wild-type) from Bacillus agaradhaerens, or variants thereof; GH family 5 glycosyl hydrolases (wild-type) from Paenibacillus, such as XYG1034 and XYG1022 as described in U.S. Pat. No. 6,630,340, or variants thereof; GH family 74 glycosyl hydrolases (wild-type) from Jonesia sp, such as XYG1020 as described in WO 2002 / 077242, or variants thereof; and Trichoderma reesei, such as the enzyme described in more detail in U.S. Pat. No. 7,172,891, or SEQ ID NO: 2. Suitable bacterial cleaning cellulases are sold under the trade names Celluclean® and Whitezyme® (Novozymes A / S, Bagsvaerd, Denmark).

[0078] The composition may include a fungal cleaning cellulase belonging to family 45 of glycosyl hydrolases, having a molecular weight of 17 kDa to 30 kDa, such as endoglucanases sold under the trade names Biotouch® NCD, DCC, and DCL (AB Enzymes, Darmstadt, Germany).

[0079] Pectate lyase. Other preferred enzymes include pectate lyases sold under the trade names Pectawash®, Pectaway®, Xpect®, and mannases sold under the trade names Mannaway® (all from Novozymes A / S, Bagsvaerd, Denmark), and Purabrite® (from Genencor International Inc., Palo Alto, California).

[0080] Nuclease enzyme. The composition may comprise a nuclease enzyme. A nuclease enzyme is an enzyme capable of cleaving phosphodiester bonds between nucleotide subunits of nucleic acids. The nuclease enzyme herein is preferably a deoxyribonuclease or ribonuclease enzyme or a functional fragment thereof. A functional fragment or portion refers to a portion of a nuclease enzyme that catalyzes the cleavage of phosphodiester bonds in the DNA backbone, and thus is a region of the nuclease protein that retains catalytic activity. It therefore includes truncated but functional versions of the enzyme and / or variants and / or derivatives and / or homologs in which functionality is maintained.

[0081] Preferably, the nuclease enzyme is a deoxyribonuclease preferably selected from any of the following classes: EC 3.1.21.x (wherein x=1, 2, 3, 4, 5, 6, 7, 8 or 9), EC 3.1.22.y (wherein y=1, 2, 4 or 5), EC 3.1.30.z (wherein z=1 or 2), EC 3.1.31.1, and mixtures thereof.

[0082] Mannanase. The composition may include an extracellular polymer-degrading enzyme, including a mannanase enzyme. The term "mannanase" refers to a polypeptide having mannan endo-1,4-β-mannosidase activity (EC 3.2.1.78) from glycoside hydrolase family 26, which catalyzes the hydrolysis of 1,4-3-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Other names for mannan endo-1,4-β-mannosidase include 1,4-3-D-mannan mannanohydrolase, endo-1,4-3-mannanase, endo-β-1,4-mannase, β-mannanase B, 3-1,4-mannan 4-mannanohydrolase, endo-3-mannanase, and β-D-mannanase. For purposes of this disclosure, mannanase activity may be determined using the reduced-endo assay described in the experimental section of WO2015040159. Suitable examples from classification EC3.2.1.78 are described in WO2015040159, such as the mature polypeptide SEQ ID NO: 1 described therein.

[0083] Galactanase. The composition may include an extracellular polymer-degrading enzyme, including an endo-beta-1,6-galactanase enzyme. The term "endo-beta-1,6-galactanase" or "polypeptide having endo-beta-1,6-galactanase activity" refers to endo-beta-1,6-galactanase activity (EC 3.2.1.164) from glycoside hydrolase family 30 that catalyzes the hydrolytic cleavage of 1,6-3-D-galactooligosaccharides with a degree of polymerization (DP) greater than 3 and their acidic derivatives bearing a 4-O-methyl glucosyl uronate or glucosyl uronate group at the non-reducing end. For purposes of the present disclosure, endo-beta-1,6-galactanase activity is determined in Assay I according to the procedure described in WO2015185689. Suitable examples from the EC3.2.1.164 classification are described in WO2015185689, e.g., mature polypeptide SEQ ID NO: 2.

[0084] Enzyme Stabilizing System. The composition may optionally comprise from about 0.001% to about 10% by weight of the composition of an enzyme stabilizing system. The enzyme stabilizing system may be any stabilizing system compatible with detersive enzymes. In the case of aqueous detergent compositions containing proteases, reversible protease inhibitors such as boron compounds, including borate, 4-formylphenylboronic acid, phenylboronic acid, and derivatives thereof, or compounds such as calcium formate, sodium formate, and 1,2-propanediol may be added to further improve stability.

[0085] Builders. The compositions may optionally include a builder or builder system. Built cleaning compositions typically include at least about 1% builder by weight, based on the total weight of the composition. Liquid cleaning compositions may include up to about 10%, and in some instances up to 8%, builder by weight of the composition. Granular cleaning compositions may include up to about 30%, and in some instances up to 5%, builder by weight of the composition.

[0086] Builders selected from aluminosilicates (e.g., zeolite builders such as zeolite A, zeolite P, and zeolite MAP) and silicates assist in controlling mineral hardness, especially calcium and / or magnesium, in wash water or in removing particulate soils from surfaces. Suitable builders may be selected from the group consisting of phosphates, such as polyphosphates (e.g., sodium tri-polyphosphate), especially its sodium salt; carbonates, bicarbonates, sesquicarbonates, and carbonate minerals other than sodium carbonate or sesquicarbonates; organic mono-, di-, tri-, and tetracarboxylates, especially water-soluble non-surfactant carboxylates in the form of acid, sodium, potassium, or alkanolammonium salts, as well as oligomeric or water-soluble low-molecular-weight polymeric carboxylates, including aliphatic and aromatic species, and phytic acid. These may be complemented, for example, by borates for pH buffering purposes, or by sulfates, especially sodium sulfate, and any other fillers or carriers that may be important in engineering stable surfactant- and / or builder-containing cleaning compositions. Additional suitable builders may be selected from citric acid, lactic acid, fatty acids, polycarboxylate builders, such as copolymers of acrylic acid, copolymers of acrylic acid and maleic acid, and copolymers of acrylic acid and / or maleic acid and other suitable ethylenic monomers with various types of additional functional groups. Also suitable for use as builders herein are synthetic crystalline ion exchange materials or hydrates thereof having a chain structure and a composition represented by the following general anhydrous form: x(MO)·ySiO·zMO, where M is Na and / or K, M' is Ca and / or Mg, y / x is 0.5 to 2.0, and z / x is 0.005 to 1.0.

[0087] Alternatively, the composition may be substantially free of builders.

[0088] Chelating Agents. The compositions may also include one or more metal ion chelating agents. Suitable molecules include copper, iron, and / or manganese chelating agents and mixtures thereof. Such chelating agents may be selected from the group consisting of phosphonates, aminocarboxylates, aminophosphonates, succinates, polyfunctionally substituted aromatic chelating agents, 2-pyridinol-N-oxide compounds, hydroxamic acids, carboxymethyl inulin, and mixtures thereof. The chelating agents may be present in acid form or in salt form, including alkali metal salts, ammonium salts, and substituted ammonium salts thereof, and mixtures thereof.

[0089] Aminocarboxylates useful as chelating agents include ethylenediaminetetracetate (EDTA), N-(hydroxyethyl)ethylenediaminetriacetate (HEDTA), nitrilotriacetate (NTA), ethylenediaminetetraproprionate, triethylenetetraaminehexaacetate, diethylenetriaminepentaacetate (DTPA), methylglycinediacetic acid (MGDA), glutamic acid diacetate (GLDA), ethanoldiglycine, triethylenetetraaminehexaacetic acid (TTHA), N-hydroxyethyliminodiacetic acid (HEIDA), dihydroxyethylglycine (DHEG), ethylenediaminetetrapropionic acid (ETPA), and the like. acid, EDTP), and derivatives thereof.

[0090] Carboxylate polymer. The composition may include one or more carboxylate polymers as a polymeric dispersant, anti-redeposition agent, or cleaning polymer. The carboxylate polymer may include at least one monomer selected from acrylic acid, maleic acid (or maleic anhydride), fumaric acid, itaconic acid, aconitic acid, mesaconic acid, citraconic acid, methylenemalonic acid, and any mixture thereof. In some embodiments, suitable carboxylate polymers include maleate / acrylate random copolymers or polyacrylate homopolymers.

[0091] In another embodiment, the carboxylate polymer can further comprise other monomers. Suitable other monomers can include sulfonated monomers such as 2-acrylamido-2-methylpropanesulfonic acid (AMPS), 2-(meth)acrylamido-2-methylpropanesulfonic acid, 4-styrenesulfonic acid, vinylsulfonic acid, 3-allyloxy, 2-hydroxy-1-propanesulfonic acid (HAPS), 2-sulfoethyl(meth)acrylic acid, 2-sulfopropyl(meth)acrylic acid, 3-sulfopropyl(meth)acrylic acid, and 4-sulfobutyl(meth)acrylic acid, and salts thereof.

[0092] Suitable other monomers may also include hydrophobically modified monomers such as alkyl acrylates, or monomers represented by formulas (I) and (II).

[0093] [ka] In formula (I), R0 represents a hydrogen atom or a CH3 group, R represents a CH2 group, a CH2CH2 group, or a single bond, X represents a number from 0 to 5, provided that when R is a single bond, X represents a number from 1 to 5, and R1 represents a hydrogen atom or a C1 to C20 organic group,

[0094] [ka] In formula (II), R0 represents a hydrogen atom or a CH3 group, R represents a CH2 group, a CH2CH2 group or a single bond, X represents a number of 0 to 5, and R1 represents a hydrogen atom or a C1 to C 20 It is an organic group.

[0095] Amphiphilic cleaning polymers. The compositions have the following general structure: bis((C2H5O)(C2H4O)n)(CH3)-N + -C x H 2x -N + It may include one or more amphiphilic cleaning polymers such as a compound having the formula -(CH3)-bis((C2H5O)(C2H4O)n) where n=20-30 and x=3-8, or sulfated or sulfonated variants thereof.

[0096] The compositions may include amphiphilic alkoxylated grease cleaning polymers that have balanced hydrophilic and hydrophobic properties to remove grease particles from fabrics and surfaces. Specific embodiments of amphiphilic alkoxylated grease cleaning polymers include a core structure and a plurality of alkoxylate groups attached to the core structure. These may include, for example, alkoxylated polyalkyleneimines having an inner polyethylene oxide block and an outer polypropylene oxide block.

[0097] Alkoxylated polyamines can be used for grease and particulate removal. Such compounds include, but are not limited to, ethoxylated polyethyleneimine, ethoxylated hexamethylenediamine, and their sulfated derivatives. Polypropoxylated derivatives can also be included. A wide variety of amines and polyalkyleneimines can be alkoxylated to various degrees. A useful example is a 600 g / mole polyethyleneimine core ethoxylated to 20 EO groups per NH, available from BASF.

[0098] The cleaning composition may comprise a hydrophilic backbone comprising monomers such as, for example, unsaturated C1-C6 carboxylic acids, ethers, alcohols, aldehydes, ketones, esters, sugar units, alkoxy units, maleic anhydride, saturated polyalcohols such as glycerol, and mixtures thereof, and one or more C4-C6 carboxylic acids, for example, unsaturated C1-C6 carboxylic acids, ethers, alcohols, aldehydes, ketones, esters, sugar units, alkoxy units, maleic anhydride, saturated polyalcohols such as glycerol, and mixtures thereof. 25 Examples of such graft polymers include random graft polymers containing hydrophobic side chains such as alkyl groups, polypropylene, polybutylene, vinyl esters of saturated C1-C6 monocarboxylic acids, C1-C6 alkyl esters of acrylic or methacrylic acid, and mixtures thereof. Specific examples of such graft polymers are based on polyalkylene oxides and vinyl esters, particularly vinyl acetate. These polymers are typically prepared by polymerizing vinyl esters in the presence of polyalkylene oxides, with initiators such as dibenzoyl peroxide, dilauroyl peroxide, or diacetyl peroxide.

[0099] The cleaning composition may contain blocks of ethylene oxide and propylene oxide. Examples of such block polymers include ethylene oxide-propylene oxide-ethylene oxide (EO / PO / EO) triblock copolymers, where the copolymer contains a first EO block, a second EO block, and a PO block, and the first EO block and the second EO block are connected to the PO block. The ethylene oxide, propylene oxide, and butylene oxide blocks may also be arranged in other ways, such as (EO / PO) diblock copolymers and (PO / EO / PO) triblock copolymers. The block polymer may also contain an additional butylene oxide (BO) block.

[0100] Cellulosic Polymer: The composition may comprise from about 0.1% to about 10% of a cellulosic polymer, by weight of the composition.

[0101] Suitable cellulosic polymers include alkyl celluloses, alkyl alkoxyalkyl celluloses, carboxyalkyl celluloses, and alkyl carboxyalkyl celluloses. In some embodiments, the cellulosic polymer is selected from carboxymethyl cellulose, methyl cellulose, methylhydroxyethyl cellulose, methylcarboxymethyl cellulose, and mixtures thereof. In some embodiments, the cellulosic polymer is carboxymethyl cellulose having a degree of carboxymethyl substitution of about 0.5 to about 0.9 and a molecular weight of about 100,000 Da to about 300,000 Da.

[0102] Carboxymethylcellulose polymers include hydrophobically modified carboxymethylcelluloses such as Finnfix® GDA (sold by CP Kelko), an alkylketene dimer derivative of carboxymethylcellulose sold, for example, under the trade name Finnfix® SH1 (CP Kelko), or block-based carboxymethylcelluloses sold under the trade name Finnfix® V (sold by CP Kelko).

[0103] Suitable cellulose-based polymers also include cellulose polymers with cationic and / or hydrophilic modifications. Suitable cationically modified cellulose polymers include UCARE JR125, UCARE JR400, UCARE JR30M, UCARE LR400, UCARE LR30M, SOFTCAT SL-5, SOFTCAT SL-30, SOFTCAT SL-60, SOFTCAT SL-100, SOFTCAT SX-400X, SOFTCAT SX-1300H, SOFTCAT SX-1300X, SOFTCAT SK-H, and SOFTCAT SK-MH, all of which are sold by The Dow Chemical.

[0104] Additional Amines: Various additional amines may be used in the compositions to enhance the removal of grease and particles from soiled materials. The compositions may contain from about 0.1% to about 10%, in some instances from about 0.1% to about 4%, and in other instances from about 0.1% to about 2%, of an additional amine, by weight of the cleaning composition. Non-limiting examples of additional amines may include, but are not limited to, polyamines, oligoamines, triamines, diamines, pentamines, tetraamines, or combinations thereof. Specific examples of suitable additional amines include tetraethylenepentamine, triethylenetetraamine, diethylenetriamine, or mixtures thereof.

[0105] Dye Transfer Inhibitors. The composition may further comprise one or more dye transfer inhibitors. Suitable dye transfer inhibitors include, for example, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidone, polyvinylimidazole, manganese phthalocyanine, peroxidase, polyvinylpyrrolidone polymers, ethylenediaminetetraacetic acid (EDTA); diethylenetriaminepentamethylenephosphonic acid (DTPMP); hydroxyethanediphosphonic acid (HEDP); ethylenediamine N,N'-disuccinic acid (EDDS); methylglycine diacetic acid (MGDA); diethylenetriaminepentaacetic acid (DTPA); propylenediaminetetraacetic acid (PDT A); 2-hydroxypyridine-N-oxide (HPNO); or methylglycine diacetate (MGDA); glutamic acid N,N-diacetate (N,N-dicarboxymethylglutamic acid tetrasodium salt (GLDA); nitrilotriacetic acid (NTA); 4,5-dihydroxy-m-benzenedisulfonic acid; citric acid and any salt thereof; N-hydroxyethylethylenediaminetriacetic acid (HEDTA), triethylenetetraaminehexaacetic acid (TTHA), N-hydroxyethyliminodiacetic acid (HEIDA), dihydroxyethylglycine (DHEG), ethylenediaminetetrapropionic acid (EDTP), and derivatives thereof, or combinations thereof.

[0106] Bleaching Compounds, Bleaching Agents, Bleach Activators, and Bleach Catalysts. The compositions described herein may include a bleaching agent, a bleach activator, and / or a bleach catalyst. The bleaching component may be present in a concentration of about 1% to about 30% by weight, and in some examples, about 5% to about 20% by weight, based on the total weight of the composition. When present, the amount of bleach activator may be about 0.1% to about 60% by weight, and in some examples, about 0.5% to about 40% by weight of the composition.

[0107] Examples of bleaching agents include oxygen bleaches, perborate bleaches, percarboxylic acid bleaches and their salts, peroxygen bleaches, persulfate bleaches, percarbonate bleaches, and mixtures thereof.

[0108] In some examples, the composition may also include a transition metal bleach catalyst.

[0109] Bleaching agents other than oxygen bleaches are also known in the art and can be used in the compositions. These include, for example, photoactivated bleaches, or preformed organic peracids such as peroxycarboxylic acids or their salts, or peroxysulfonic acids or their salts. A suitable organic peracid is phthaloylimoperoxycaproic acid. When used, the compositions typically contain such bleaches, and in some instances, zinc phthalocyanine sulfonate, in amounts of about 0.025% to about 1.25% by weight of the composition.

[0110] Brightening Agents Optical brighteners or other brightening or whitening agents may be incorporated at a level of from about 0.01% to about 1.2% by weight of the composition.

[0111] Commercially available optical brighteners that may be used herein can be divided into subgroups that include, but are not necessarily limited to, derivatives of stilbenes, pyrazolines, coumarins, benzoxazoles, carboxylic acids, methine cyanines, dibenzothiphene-5,5-dioxide, azoles, 5- and 6-membered heterocycles, and various other agents.

[0112] In some examples, the optical brightener is disodium 4,4'-bis{[4-anilino-6-morpholino-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (brightener 15, commercially available under the trade name Tinopal AMS-GX by Ciba-Geigy Corporation), disodium 4,4'-bis{[4-anilino-6-(N-2-bis-hydroxyethyl)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (brightener 15, commercially available under the trade name Tinopal UNPA-GX by Ciba-Geigy Corporation), disodium 4,4'-bis{[4-anilino-6-(N-2-hydroxyethyl-N-methylamino)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (brightener 15, commercially available under the trade name Tinopal AMS-GX by Ciba-Geigy Corporation), disodium 4,4'-bis{[4-anilino-6-(N-2-hydroxyethyl-N-methylamino)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (brightener 15, commercially available under the trade name Tinopal UNPA-GX by Ciba-Geigy Corporation), or disodium 4,4'-bis{[4-anilino-6-(N-2-hydroxyethyl-N-methylamino)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (brightener 15, commercially available under the trade name Tinopal AMS-GX by Ciba-Geigy Corporation). More preferably, the optical brightener is disodium 4,4'-bis{[4-anilino-6-morpholino-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate.

[0113] The whitening agent may be added in particulate form or as a premix with a suitable solvent, such as a non-ionic surfactant, monoethanolamine, propanediol.

[0114] Fabric Hueing Agents. The composition may include a fabric hueing agent (sometimes referred to as a tinting agent, bluing agent, or whitening agent). Typically, the hueing agent imparts a blue or blue-purple hue to the fabric. Hueing agents can be used either alone or in combination to create a particular hue and / or tint different types of fabric. This can be achieved, for example, by mixing a red and a green-blue dye to produce a blue or purple hue. The hueing agent may be selected from any known chemical class of dyes, including, but not limited to, acridines, anthraquinones (including polycyclic quinones), azines, azos including premetallized azos (e.g., monoazos, diazos, trisazos, tetrakisazos, polyazos), benzodifurans and benzodifuranones, carotenoids, coumarins, cyanines, diazahemicyanines, diphenylmethanes, formasans, hemicyanines, indigoids, methanes, naphthalimides, naphthoquinones, nitro and nitroso, oxazines, phthalocyanines, pyrazoles, stilbenes, styryls, triarylmethanes, triphenylmethanes, xanthenes, and mixtures thereof.

[0115] Encapsulation. The composition may include an encapsulating agent. The encapsulating agent may include a core and a shell having an inner surface and an outer surface, the shell encapsulating the core.

[0116] In certain embodiments, the encapsulating agent comprises a core and a shell, wherein the core comprises a material selected from fragrances, whitening agents, dyes, insect repellents, silicones, waxes, fragrances, vitamins, fabric softeners, skin care agents such as paraffin, enzymes, antibacterial agents, bleaching agents, sensates, or mixtures thereof, and the shell comprises a material selected from polyethylene, polyamide, polyvinyl alcohol optionally containing other comonomers, polystyrene, polyisoprene, polycarbonate, polyester, polyacrylate, polyolefin, polysaccharides such as alginate and / or chitosan, gelatin, shellac, epoxy resins, vinyl polymers, water-insoluble inorganic materials, silicones, amino resins, or mixtures thereof. In some embodiments where the shell comprises an aminoplast, the aminoplast comprises polyurea, polyurethane, and / or polyureaurethane. The polyurea may comprise polyoxymethylene urea and / or melamine formaldehyde.

[0117] Other Ingredients. The composition may further comprise a silicate. Suitable silicates may include, for example, sodium silicate, sodium disilicate, sodium metasilicate, crystalline phyllosilicates, or combinations thereof. In some embodiments, the silicate may be present at a concentration of about 1% to about 20% by weight, based on the total weight of the composition.

[0118] The composition may further comprise conventional detergent ingredients such as fabric conditioners, clays, suds boosters, suds suppressors, corrosion inhibitors, soil suspending agents, soil anti-redeposition agents, dyes, disinfectants, anti-tarnish agents, optical brighteners, or fragrances.

[0119] The composition optionally comprises a saturated or unsaturated fatty acid, preferably a saturated or unsaturated C 12 ~C 24The composition may further include fatty acids; deposition aids such as polysaccharides, cellulose polymers, polydiallyldimethylammonium halide (DADMAC) and random or block copolymers of DADMAC with vinylpyrrolidone, acrylamide, imidazole, imidazolinium halide, and mixtures thereof, cationic guar gum, cationic cellulose, cationic starch, cationic polyacrylamide, or combinations thereof. When present, the fatty acids and / or deposition aids may each be present in an amount of 0.1% to 10% by weight, based on the total weight of the composition.

[0120] The composition may optionally include silicone or fatty acid based suds suppressors; hueing dyes, calcium and magnesium cations, visual signaling components, anti-foaming agents (0.001% to about 4.0% by weight, based on the total weight of the composition), and / or structurants / thickeners (0.01% to 5% by weight, based on the total weight of the composition) selected from the group consisting of di- and triglycerides, ethylene glycol distearate, microcrystalline cellulose, microfiber cellulose, biopolymers, xanthan gum, gellan gum, and mixtures thereof.

[0121] additive composition The additive compositions of the present disclosure may include additional adjunct ingredients. Such adjuncts may provide additional treatment benefits to the target fabric and / or they may act as stabilizing or processing aids to the composition. Suitable adjuncts may include chelating agents, fragrances, structuring agents, chlorine scavengers, malodor-reducing materials, organic solvents, or mixtures thereof. [Example]

[0122] Bath towel swatches with strong malodors were treated with four different products added through the wash (TTW). Product 1: Liquid laundry detergent (Tide® Original Scent, Procter & Gamble) and polyethylene glycol (PEG) beads (100% PEG). Product 2: Liquid laundry detergent andContains 99.99% by weight of PEG and 0.01% by weight of Bacillus spores. Ruby Size. Product 3: Liquid laundry detergent and Saccharomyces supernatant (supplied as Saccharomyces cerevisiae fermentation filtrate under the product name DeoPlex® N17733 by Carrubba Incorporated, Milford, CT 06460, USA). Product 4: Liquid laundry detergent and Contains 99.99% by weight of PEG and 0.01% by weight of Bacillus spores. Ruby ze and Saccharomyces supernatant .

[0123] The swatches were machine washed, dried, and rebloomed after 24 and 48 hours and evaluated for malodor. Treatment was performed on the wash cycle, using 2360 ppm laundry detergent, and 5.5 x 10 6 Colony-forming units (CFU) and 1867 ppm Saccharomyces supernatant were used. After washing, the towel swatches were air-dried and then individually placed in sealed sterile plastic cups for 24 hours or overnight for malodor evaluation. Prior to olfactory evaluation of malodor, the towel swatches in the plastic cups were rebloomed by spraying them with deionized water equivalent to 33% of the fabric weight in the cup, and then incubated at 37°C for 1 hour. The towel swatches in the plastic cups were then allowed to equilibrate at room temperature before evaluation. Volunteer evaluators, selected from those familiar with bath towel malodor, were asked to rank the bath towels from low to high malodor from each of four sets of dedicated cups. Eight people evaluated 32 samples. After evaluation, the towel swatches were left in the cups at ambient temperature for another 24 hours, and then a second evaluation was conducted at 48 hours. The towel swatches were rebloomed a second time by spraying them with deionized water equivalent to 33% of the weight of the fabric in the cup, then incubated at 37° C. for 1 hour, then allowed to equilibrate at room temperature before evaluation.

[0124] The table below shows the malodor evaluation results, ranked numerically from low malodor (1) to high malodor (4) for the four products. As shown, towel swatches washed with Product 4 (in accordance with the present invention) show synergistic malodor reduction at 24 and 48 hours.

[0125] [Table 1]

[0126] Dimensions and values ​​disclosed herein should not be understood as being strictly limited to the exact numerical values ​​recited. Instead, unless otherwise indicated, each such dimension is intended to mean both the recited value and a functionally equivalent range surrounding that value. For example, a dimension disclosed as "40 mm" is intended to mean "about 40 mm." [1] 1. A method of treating a surface, comprising: a) bacterial spores; b) Saccharomyces supernatant; The method includes the step of treating with. [2] The method according to [1], wherein the bacterial spores include Bacillus spores. [3] The bacillus may be any of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus tequilensis, Bacillus vallismortis, Bacillus mojavensis, Bacillus mojavensis), and mixtures thereof, preferably selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, and mixtures thereof. [4] The method according to any one of [1] to [3], wherein the surface is a soft surface or a hard surface. [5] The method according to any one of [1] to [4], wherein the surface is fabric. [6] The method according to [5], wherein the treatment step is a laundry process including washing, rinsing, and drying cycles, and the bacterial spores and / or the Saccharomyces supernatant are applied to the fabric separately or together from a cleaning composition and / or an additive composition. [7] The method according to [6], wherein the Saccharomyces supernatant is supplied to the washing step and the bacterial spores are supplied to the rinsing or drying step. [8] The method according to [7], wherein the treatment step comprises adding the Saccharomyces supernatant to form an aqueous solution containing about 0.01% by weight to about 10% by weight, preferably about 0.01% by weight to about 5% by weight, of the Saccharomyces supernatant. [9] The bacterial spores are about 1 x 10 per kilogram of fabric weight. 2 CFU ~ approx. 1 x 10 7 CFU, preferably about 1 x 10 2 CFU ~ approx. 1 x 10 7 The method according to [7] or [8], wherein the anti-viral agent is added from the cleaning composition or additive composition at a concentration of CFU.

[10] A product, a) Bacterial spores and b) Saccharomyces supernatant; Including, product.

[11] The product according to

[10] , wherein the product comprises a cleaning composition and / or an additive composition.

[12] The cleaning composition and / or the additive composition may comprise about 1×10 2 ~Approx. 1×10 9

[11] The product according to

[11] , comprising CFU / g of bacterial spores and about 0.05% to about 10% by weight of the composition of Saccharomyces supernatant.

[13] The product of

[11] or

[12] , wherein the cleaning composition is in the form of a liquid, solid, or unit dose form, and the additive composition is in the form of a cleaning additive, a rinse additive, or a drying additive.

[14] The product according to any one of

[11] to

[13] , wherein the cleaning composition comprises a surfactant system including an ionic surfactant and a nonionic surfactant.

[15] The product is a laundry composition, the laundry composition comprising: a) about 5% to about 55% by weight of the composition of an ionic surfactant, preferably an anionic surfactant; b) about 5% to about 50% by weight of the composition of a nonionic surfactant; c) about 1×10 of said composition 2 ~Approx. 1×10 9 CFU / g of bacterial spores and d) about 0.05% to about 10% by weight of the composition of Saccharomyces supernatant; e) adjuvants including one or more of additional enzymes, peroxy compounds, bleach activators, anti-redeposition agents, neutralizing agents, optical brighteners, suds suppressors, chelating agents, bittering agents, dye transfer inhibitors, soil release agents, water softeners, electrolytes, pH adjusters, graying inhibitors, anti-wrinkle ingredients, bleaching agents, colorants, fragrances, processing aids, and mixtures thereof; The product according to any one of

[10] to

[14] , including:

[16] 1. The method of treatment according to any one of [1] to [9] or the use of the product according to any one of

[10] to

[15] to provide sustained malodour removal to a substrate, preferably a fabric.

Claims

1. A method for treating a fabric surface, comprising: a) Bacillus spores; b) Saccharomyces supernatant; and a treatment step of treating with The Bacillus is selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus tequilensis, Bacillus vallismortis, Bacillus mojavensis, and mixtures thereof.

2. 10. The method of claim 1, wherein the treatment step is a laundry process including wash, rinse, and dry cycles, and the Bacillus spores and / or the Saccharomyces supernatant are applied to the fabric separately or together from a cleaning composition and / or an additive composition.

3. 3. The method of claim 2, wherein the Saccharomyces supernatant is provided to the wash and the Bacillus spores are provided to the wash in the form of beads.

4. 3. The method of claim 2, wherein the Saccharomyces supernatant is provided to the washing step and the Bacillus spores are provided to the rinsing or drying step.

5. 5. The method of claim 4, wherein the treating step comprises adding the Saccharomyces supernatant to form an aqueous solution containing 0.01% to 10% by weight of the Saccharomyces supernatant.

6. The Bacillus spores are present in an amount of 1 x 10 per kilogram of fabric weight. ２ CFU ~ 1 x 10 ７ 5. The method of claim 4, wherein the antibacterial agent is added from a cleaning composition or an additive composition at a concentration of CFU.

7. A fabric treatment product comprising: a) Bacillus spores and b) Saccharomyces supernatant; and Including, 1. A fabric treatment product wherein the Bacillus is selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus tequilensis, Bacillus vallismortis, Bacillus mojavensis, and mixtures thereof.

8. 8. The fabric treatment product of claim 7, wherein the fabric treatment product comprises a cleaning composition and / or an additive composition.

9. The cleaning composition and / or the additive composition may be 1×10 ２ ~1 x 10 ９ 9. The fabric treatment product of claim 8, comprising CFU / g of Bacillus spores and 0.05% to 10% by weight of the composition of Saccharomyces supernatant.

10. 10. The fabric treatment product of claim 8 or 9, wherein the cleaning composition is in the form of a liquid, solid, or unit dose form, and the additive composition is in the form of a wash additive, a rinse additive, or a dry additive.

11. 10. A fabric treatment product according to claim 8 or 9, wherein the cleaning composition comprises a surfactant system comprising an ionic surfactant and a non-ionic surfactant.

12. 10. The fabric treatment product of claim 8, wherein the additive composition comprises the Bacillus spores and polyethylene glycol and is in the form of beads.

13. The fabric treatment product is a laundry composition, the laundry composition comprising: a) 5% to 55% by weight of the composition of an ionic surfactant; b) 5% to 50% by weight of the composition of a nonionic surfactant; c) 1 x 10 of the composition ２ ~1 x 10 ９ CFU / g of Bacillus spores; d) 0.05% to 10% by weight of the composition of Saccharomyces supernatant; e) adjuvants including one or more of additional enzymes, peroxy compounds, bleach activators, anti-redeposition agents, neutralizing agents, optical brighteners, suds suppressors, chelating agents, bittering agents, dye transfer inhibitors, stain release agents, water softeners, electrolytes, pH adjusters, graying inhibitors, anti-wrinkle ingredients, bleaching agents, colorants, fragrances, processing aids, and mixtures thereof; 8. The fabric treatment product of claim 7, comprising:

14. Use of the method of treatment of claim 1 or the fabric treatment product of claim 7 to provide sustained odour removal to fabrics.

Images