Pharmaceutical Composition

Abstract

To provide pharmaceutical compositions for treating cancer including glioma.SOLUTION: Provided is a pharmaceutical composition for treating glioma in a patient who has been previously treated with temozolomide, comprising: (a) a therapeutically effective amount of eflornithine or a pharmaceutically acceptable salt of eflornithine; and (b) a pharmaceutically acceptable carrier, the glioma having a mutation in one or more genes selected from the group consisting of IDH1, IDH2, TP53, PTEN, and ATRX, and the pharmaceutical composition slowing the progression of the glioma.SELECTED DRAWING: None

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 312,623, filed March 24, 2016, by Victor A. Levin, M.D., entitled "Compositions of Eflornithine and Its Derivatives and Analogs and Methods of Use Thereof for Treating Cancer, Including Glioma," the contents of which are incorporated herein by reference in their entirety. [Technical Field]

[0002] The present invention relates to compositions and methods of use of eflornithine and its derivatives and analogs for treating cancer, including glioma. [Background technology]

[0003] Gliomas are one of the most common and serious forms of brain tumor. Gliomas are classified by cell type, grade, and location. Gliomas are generally named according to the specific cell type(s) they share histological characteristics with, but not necessarily the cell type from which they originate. The main types of gliomas are ependymomas (ependymal cells), astrocytomas (astrocytic cells), oligodendroglioma (oligodendroglioma), brainstem gliomas (brainstem), optic nerve gliomas (cells in or surrounding the optic nerve), and mixed gliomas (cells derived from different types of glia). Gliomas are further characterized according to grade, generally determined according to the WHO classification. Grade I is the least aggressive grade, representing the least progressive disease with the best prognosis; grade I gliomas are generally considered benign. Grade II in the WHO classification is the next least aggressive grade. Grade II gliomas are well differentiated and not anaplastic. These gliomas tend to be benign and may have a favorable prognosis, but they tend to recur and become higher grade, i.e., more severe, over time. High-grade gliomas, grades III and IV, according to the WHO classification, are undifferentiated or anaplastic and clearly malignant. These grades carry the worst prognosis. Gliomas can also be classified according to their location, specifically whether they are located above or below the membrane, or tentorium, in the brain. The tentorium separates the cerebrum from the cerebellum. Supratentorial gliomas occur more frequently in adults, while infratentorial gliomas occur more frequently in children. Certain types of gliomas, such as subependymoma or juvenile pilocytic astrocytoma (JPA), tend to be non-invasive or minimally invasive.

[0004] Symptoms of gliomas generally vary depending on which part of the central nervous system is affected. Gliomas in the brain can cause headaches, vomiting, seizures, focal collapse, impaired new memory formation, speech problems, and cranial nerve damage as a result of tumor growth. Gliomas of the optic nerve can cause visual impairment or blindness. Gliomas of the spinal cord can cause pain, weakness, or paralysis in one or more extremities. Gliomas generally do not metastasize through the bloodstream but can spread throughout the cerebrospinal fluid and induce instillation in the spinal cord.

[0005] The exact cause of gliomas is unknown. Certain inherited genetic disorders, such as neurofibromatosis type 1 or type 2 or tuberous sclerosis, may predispose to glioma growth. Several oncogenes may be involved in the development and growth of gliomas. Many gliomas are infected with cytomegalovirus, which may accelerate their growth. Germline (inherited) polymorphisms in the DNA repair genes ERCC1, ERCC2 (XPD), and XRCC1 may increase the risk of gliomas. This is due to altered or defective repair of DNA damage. Studies have shown that insufficient DNA repair may contribute to glioma formation. Excessive DNA damage can lead to mutations through translesion synthesis. Furthermore, incomplete DNA repair can result in epigenetic changes or epimutations. Such mutations and epimutations may provide cells with a growth advantage that can subsequently lead to cancer progression through the process of natural selection. Epigenetic suppression of DNA repair genes is often found during the progression to sporadic glioblastoma. For example, methylation of the DNA repair gene MGMT promoter has been observed in a significant proportion of glioblastomas. Furthermore, in some glioblastomas, MGMT protein may be deficient due to other types of epigenetic changes. MGMT protein expression may also be reduced by increased levels of microRNAs that inhibit the ability of MGMT messenger RNA to produce MGMT protein. In glioblastomas lacking MGMT promoter methylation, the level of the microRNA miR-181d was found to be inversely correlated with MGMT protein expression, and the direct target of miR-181d was found to be the MGMT mRNA 3'UTR. Epigenetic reduction in the expression of another DNA repair protein, ERCC1, has been observed in many gliomas, and in some cases, this reduction has been attributed to reduced or absent ERCC1 protein expression. In other cases, the reduction was due to methylation of the ERCC1 promoter. In a few cases, the reduction may have been due to epigenetic changes in microRNAs that affect ERCC1 expression. Reduced expression of DNA repair genes can lead to high levels of DNA damage accumulation in cells. In gliomas, mutations frequently occur in the isocitrate dehydrogenase genes IDH1 and IDH2. These mutations can result in the production of excess metabolic intermediate 2-hydroxyglutarate, which binds to the catalytic site of key enzymes important in histone modification and DNA promoter methylation.This can result in DNA CpG island methylation phenotypes (CIMPs), which can induce promoter hypermethylation and simultaneous silencing of tumor suppressor genes such as the DNA repair genes MGMT and ERCC1. Furthermore, mutations in IDH1 and IDH2 can increase oxidative stress and therefore lead to increased oxidative damage to DNA.

[0006] Several acquired genetic mutations are commonly found in gliomas, including mutations in p53 and PTEN, and sometimes loss of the gene encoding PTEN. These mutations can lead to overexpression of EGFR. However, hypermutations associated with gliomas are not restricted to specific locations.

[0007] High-grade glioma is a highly vascular tumor and tends to invade.It has extensive necrosis and hypoxic areas.In many cases, tumor growth induces the destruction of the blood-brain barrier near the tumor.Usually, even after complete surgical removal, high-grade glioma will almost always grow again, which is commonly referred to as recurrent brain cancer.In contrast, low-grade glioma typically grows relatively slowly, and if glioma does not grow or cause symptoms, it can be monitored without the need for active treatment.

[0008] Treatment for gliomas varies depending on location, cell type, and grade. Combination therapy, including surgical resection, radiation therapy, and chemotherapy, is often used. One frequently used therapeutic agent is temozolomide, which can cross the blood-brain barrier and is frequently used in the treatment of high-grade gliomas. The monoclonal antibody, angiogenesis blocker bevacizumab, is also frequently used. However, there is growing evidence that the use of temozolomide may itself induce mutations and worsen the prognosis in a significant proportion of patients (B.E. Johnson et al., "Mutational Analysis Reveals the Origin and Therapy-Driving ven Evolution of Recurrent Glioma,” Science 343:189-193 (2014), incorporated herein by reference. The potential mutagenic effects of temozolomide must be considered when planning a course of treatment for glioma.

[0009] Gliomas are rarely curable. The prognosis for patients with high-grade gliomas is generally poor, especially in older patients. Based on CBTRUS (Table 23, 2015 edition), of the 10,000 Americans diagnosed with malignant gliomas each year, approximately 57% survive one year after diagnosis, approximately 41% survive two years, and only 31% survive five years. For patients with anaplastic astrocytomas, the survival rate is approximately 44% at two years and approximately 28% at five years. For glioblastoma multiforme, the prognosis is even worse, with a 37% survival rate one year after diagnosis and a 15% survival rate two years after diagnosis. For low-grade gliomas, the prognosis is somewhat more optimistic, but even these patients have a significantly higher mortality rate than the general population, taking age into account.

[0010] Therefore, there is a great need for improved treatments for gliomas. Furthermore, there is a particular need for treatments that can avoid or counteract the potentially mutagenic effects of commonly used anti-neoplastic drugs, such as temozolomide. As described in more detail below, the principles of the treatments provided by the present invention can also be applied to malignant tumors in general, as cancers are typically characterized by mutations of neoplastic cells. Summary of the Invention

[0011] The present invention provides a novel therapeutic approach for the treatment of glioma.

[0012] One aspect of the present invention is a method for treating glioma, comprising administering a therapeutically effective amount of eflornithine or its derivative or analog to a subject suffering from glioma, so as to reduce the mutation rate of glioma and reduce the progression of glioma.Typically, glioma is WHO grade I, grade II, grade III or grade IV glioma.In one embodiment, glioma is selected from the group consisting of anaplastic glioma, anaplastic oligodendroglioma and mixed anaplastic oligoastrocytoma.The use of eflornithine is disclosed in U.S. Patent No. 6,553,351 by Levin.

[0013] In one embodiment, eflornithine or a derivative or analog thereof is eflornithine, such as a racemic mixture of D-eflornithine and L-eflornithine, D-eflornithine, or L-eflornithine. In another embodiment, eflornithine or a derivative or analog thereof is a derivative or analog of eflornithine.

[0014] Typically, eflornithine or its derivatives or analogs are administered in conjunction with an alkylating agent to reduce the mutation rate of gliomas. The alkylating agent may be temozolomide or another conventional alkylating agent. Alkylating agents are mutagenic to some extent.

[0015] Eflornithine or a derivative or analog thereof can be administered orally or by injection.

[0016] In one embodiment, the glioma has been previously treated with radiation therapy and adjuvant alkylating agent therapy and is a recurrent / refractory anaplastic glioma. Radiation therapy is routinely used for gliomas (M.D. Prados et al., "Phase III Trial of Accelerated Hyperfractionation with or without Difluromethylornithine (DFMO) Versus Standard Fractionated Radiotherapy"). therapy with or without DFMO for Newly Diagnosed Patients with Glioblastoma Multiforme,” Int. J. Rad. Oncol. Biol. Phys. 49:71-77(2001)).

[0017] Glioma may have mutations in one or more genes selected from the group consisting of IDH1, IDH2, TP53, PTEN and ATRX. Glioma may have methylated MGMT promoter. One form of glioma that can be treated is astrocytoma.

[0018] Eflornithine or its derivatives or analogs can be administered together with a therapeutically effective amount of one or more conventional antineoplastic agents used in the treatment of glioma.The one or more conventional antineoplastic agents used in the treatment of glioma can be selected from the group consisting of alkylating agents, antimetabolites, angiogenesis inhibitors, EGFR inhibitors, platinum-containing agents, topoisomerase inhibitors and other classes of drugs.In another embodiment, eflornithine or its derivatives or analogs are administered together with polyamine transport inhibitors.In yet another embodiment, eflornithine or its derivatives or analogs are administered together with polyamine analogs.In yet another embodiment, eflornithine or its derivatives or analogs are administered together with S-adenosylmethionine decarboxylase inhibitors. In yet another embodiment, eflornithine or a derivative or analog thereof is administered in combination with (1) a retinoid, (2) a silbactin compound, (3) a cyclooxygenase-2 inhibitor, (4) a bellinoid, (5) a nonsteroidal anti-inflammatory agent, (6) a castanospermine or a castanospermine ester, (7) an aziridinylputrescine compound, (8) an interferon, (9) an aryl-substituted xylopyranoside derivative, or (10) an agent that reduces blood glutamate levels and promotes the transport of glutamate from the brain to the blood. In yet another embodiment, eflornithine, or a derivative or analog thereof, is administered with an agent that enhances the ability of eflornithine, or a derivative or analog thereof, to cross the blood-brain barrier.

[0019] Another aspect of the present invention is a method for producing a semiconductor device comprising: (1) a therapeutically effective amount of eflornithine or an eflornithine derivative or analog; (2) optionally, a therapeutically effective amount of at least one additional agent that may be used in combination with eflornithine or an eflornithine derivative or analog; and (3) Pharmaceutically acceptable carrier wherein the composition is administered to reduce the mutation rate of gliomas and thereby reduce the progression of gliomas.

[0020] The additional agents are as described above. The pharmaceutical composition may be formulated for oral administration or for administration by injection.

[0021] Conventional pharmaceutically acceptable carriers are known in the art and include, but are not limited to, sugars, solvents, thickeners, emulsifiers, diluents, sweeteners, humectants, organic acids, colorants, flavors, and preservatives. DETAILED DESCRIPTION OF THE INVENTION

[0022] Generally, this invention relates to a method for treating eflornithine ("DFMO") alone or in combination with lomustine and other The present invention relates to the treatment of patients with relapsed / refractory anaplastic astrocytoma to temozolomide in combination with chemotherapy. In addition to eflornithine, derivatives or analogs of DFMO can be used, as described in more detail below.

[0023] Nonproteinogenic neutral amino acids (e.g., DFMO) that are also AA T6 transporter substrates can extend the lifespan of cancer patients by inhibiting the progression of DNA mutations induced by chemotherapy agents.

[0024] The basic principles of the present invention are roughly as follows: (1) chemotherapeutic agents induce DNA mutations; (2) DFMO disrupts cell proliferation and differentiation; and (3) by disrupting cell proliferation and differentiation in cancer cells, DFMO inhibits DNA mutations, thus suppressing cancer progression. Therefore, because chemotherapeutic agents induce DNA mutations, combining DFMO with these agents inhibits such mutations, thereby improving the survival of cancer patients.

[0025] Eflornithine exists in two enantiomeric forms: D-eflornithine and L-eflornithine. D-eflornithine is shown below in formula (Ia). L-eflornithine is shown below in formula (Ib).

[0026] [ka]

[0027] ; and

[0028] [ka]

[0029] Typically, eflornithine is administered as a racemic mixture of D-eflornithine and L-eflornithine, however, eflornithine can also be administered as a mixture in which D-eflornithine is relatively abundant relative to L-eflornithine, or as a pure or substantially pure preparation of D-eflornithine.

[0030] Eflornithine is a structural analog of the amino acid L-ornithine, shown below as formula (II).

[0031] [ka]

[0032] Catalysis by ornithine decarboxylase (ODC) is known to be the rate-limiting step in polyamine synthesis. The pathway for polyamine synthesis begins with L-ornithine. This natural amino acid is not normally incorporated into proteins but is part of the urea cycle, which metabolizes arginine to ornithine and urea. Ornithine is converted to putrescine and CO2 by ornithine decarboxylase (ODC), which is considered to be the rate-limiting step in polyamine production. Upon addition of propylamine provided by S-adenosylmethionine, putrescine is converted to spermidine. Spermidine is then converted to spermine by spermine synthetase, again accompanied by decarboxylation of S-adenosylmethionine. Putrescine, spermidine, and spermine represent the three major polyamines in mammalian tissues. Polyamines are found in animal tissues and microorganisms and are known to play important roles in cell growth and proliferation. Although the mechanism of action of eflornithine in the treatment of glioma is thought to primarily involve preventing the induction of mutations in tumor cells, eflornithine's effect on the synthesis of polyamines may play a secondary role.

[0033] Several derivatives and analogs of eflornithine are known in the art and are described in further detail below.

[0034] Eflornithine is an irreversible inhibitor of the enzyme ornithine decarboxylase (ODC) and was originally developed as a treatment for trypanosomiasis (1-3). Eflornithine is also being investigated as a treatment for various cancers (4). Eflornithine can be administered orally or by injection, for example, intravenously or intraperitoneally.

[0035] Although it is well established that eflornithine's primary action is to inhibit ODC activity and thereby inhibit the production of putrescine from ornithine, its pleiotropic effects as an anticancer agent have not yet been fully recognized or understood at this time. Numerous mechanisms have been proposed to explain eflornithine's effectiveness against tumor cells (4-6). Because polyamines (putrescine, spermidine, and spermine) play essential roles in DNA and RNA function, it is a long-standing theory that inhibition of ODC would inhibit tumor growth and possibly tumor cell migration. Eflornithine can also reduce the effects of chemical carcinogens in colon, skin, and bladder tissues and cell lines, as well as in clinical settings (4, 6).

[0036] However, over the past few years, new findings have emerged suggesting a different antitumor activity for eflornithine against CNS gliomas than previously recognized or suggested. The present invention relates to this new basis for antitumor activity. Specifically, We argue that the primary anticancer effect of eflornithine resides in its ability to downregulate mutations in slow-growing, invasive gliomas (WHO grade II and III tumors). By reducing the occurrence and / or number of mutations, we believe that tumor growth in patients will be halted and / or slowed because the mutationally activated drivers of cancer progression and transformation to the more aggressive glioblastoma (WHO grade IV) will fail to occur or will become fewer over time.

[0037] A phase 3 randomized clinical trial of adjuvant eflornithine-PCV versus PCV (procarbazine / CCNU / vincristine) chemotherapy in patients with anaplastic glioma (7) provided evidence for this hypothesis. The trial showed that eflornithine-PCV chemotherapy resulted in a shift in the progression-free survival (PFS) hazard function when compared with PCV chemotherapy continued approximately 1 to 1.5 years after cessation of eflornithine-PCV chemotherapy. From that point onward, the PFS and overall survival (OS) curves remained parallel and did not cross over a 10-year period (7, 8). This hypothesis includes the possibility that eflornithine may have prevented anaplastic gliomas (particularly anaplastic astrocytomas) from progressing to more aggressive phenotypes, such as glioblastomas. Further support comes from recent studies in neuroblastoma cells that found that eflornithine can increase two intracellular proteins, p21 and p27kip-1, thereby arresting cell division during G1 and the onset of mitosis (9, 10).

[0038] These two findings, taken together, suggest that eflornithine, which can be safely administered orally for 2 weeks every 3 weeks for years at a time and can induce G1 arrest and an increase in intracellular p21 and p27kip-1, may very well reduce the mutation rate in glioma tumor cells in situ, thereby providing a novel and unexpected effect on the transformation of low-grade (WHO grade II) and intermediate-grade (WHO grade III) gliomas to glioblastomas (WHO grade IV). This approach, by limiting mutations, may provide a long-term survival benefit to patients with these tumors, as demonstrated in clinical trials (7, 8). It has also been suggested that treatment with eflornithine should be continued for many years in patients with low-grade and intermediate-grade gliomas. The increase in intracellular p21 and p27kip-1 induced by eflornithine administration is associated with mutation suppression by this drug, with the clinical consequence of preventing or delaying the progression of gliomas to aggressive forms.

[0039] Further results support this hypothesis (11). These results demonstrate the importance of mutations in the transformation of low- and intermediate-grade gliomas to more aggressive tumor grades. The premise of this study was that treatment for recurrent or progressive gliomas fails because the genomic alterations driving the growth of recurrent tumors differ from those in initial tumors. In this study, exome sequencing was performed on 23 initial low-grade gliomas and recurrent tumors resected from the same patient. The three most commonly mutated genes in grade 2 gliomas at initial diagnosis were found to be IDH1 in 100% (23 / 23) of the cohort studied, TP53 in 83% (19 / 23), and ATRX in 78% (18 / 23). The next most commonly mutated gene, SMARCA4, was identified in 13% (3 / 23) of the initial tumors in this cohort. Thirteen additional genes were identified in 9% (2 / 23) of the cohort. Interestingly, in 43% of cases, at least half of the mutations in the initial tumor were not detected in tumor recurrence / progression, including driver mutations in TP53, ATRX, SMARCA4, and BRAF, suggesting that recurrent tumors may be seeded by cells derived from the initial tumor very early in their evolution. Highlighting the importance of early treatment, of further interest was the finding that tumors from 6 of 10 patients treated with adjuvant temozolomide (TMZ) chemotherapy followed an alternative progression pathway to high-grade gliomas; these tumors displayed hypermutation and harbored driver mutations in the RB and AKT-mTOR pathways with signatures of TMZ-induced mutagenesis. These studies expanded on earlier findings and studies of primary GBM (12, 13), unpaired recurrent tumors (14), and cell culture models (15).

[0040] Given that all WHO grade II and III gliomas follow a mutational pathway, if they recur or progress, one logical approach to controlling these tumors would be to reduce the rate and extent of mutations they can develop. As previously discussed, causal evidence that eflornithine reduces mutation rates in treated anaplastic glioma patients (7, 8) is currently lacking, but circumstantial evidence favors a role for eflornithine in reducing tumor cell mutations. In retrospect, eflornithine induces G1 arrest in neuroblastoma, a neuroectodermal tumor-like glioma, by increasing intracellular p21 and p27kip-1 proteins (9, 10), arguably impacting the mutation rate of tumor cells. Topical eflornithine treatment of biopsied actinic keratoses in skin was previously found to reduce the percentage of p53-positive cells (22%; P = 0.04) compared with placebo-treated skin, but not the frequency of p53 mutations (16).

[0041] This hypothesis can be evaluated by the experiment that can prove or support the conclusion that eflornithine can reduce mutation rate in low-grade glioma and medium-grade glioma.However, these tumors generally do not grow well outside of human host.One embodiment is to use one or more conventional cell lines that grow in three-dimensional culture and do not initially have many mutations.

[0042] Another approach is to use Big Blue Rat-2 cells in a manner similar to that used to evaluate the mutagenic potential of TMZ (15). In addition to examining DNA adducts, we also examined lacl mutations in Big Blue Rat-2 cells and found a dose-dependent increase in lacl mutation frequency from 9.1 to 48.9 and 89.7 upon TMZ treatment with 0, 0.5, or 1 mM TMZ. Sequence analysis of lacl mutants from the TMZ-treated groups confirmed that the mutations were GC-to-AT transitions at non-CpG sites, which is significantly different from the mutation spectrum observed in the control-treated group. Therefore, it is conceivable that Big Blue Rat-2 cells could be exposed to a defined dose and duration of TMZ exposure to initiate a mutation cascade, followed by treatment with eflornithine.

[0043] Another possible approach involves using slowly progressing rodent tumors that cause death over a period of approximately six months, examining mutations occurring at 2, 4, and 6 months, and dividing the mice into two groups. The first group received eflornithine (1.5–2%) in drinking water for 3 / 4 weeks between months 2 and 6, while the second group received no eflornithine. This approach, which involves sequencing approximately 500 genes from each tumor tissue sample obtained at euthanasia at 2, 4, and 6 months after tumor implantation, may be sufficient to provide information and evidence of the effectiveness of eflornithine on mutation frequency.

[0044] Previously described techniques (23, 24) for growing glioma or adenocarcinoma cells in 3D culture and then assessing the effects of treatment with single agents or drug combinations can be used. These techniques initially involve the extraction of phosphoproteins from 3D cultures under serum starvation or hypoxic conditions. Although developed for the rapid isolation of proteins (25, 26), these techniques will also work well for the isolation of DNA and RNA. One approach involves using the monofunctional alkylating agent temozolomide to induce mutations, followed by two doses and two administrations of eflornithine to determine how well eflornithine reduces the mutation frequency of tumor cells. Because temozolomide and eflornithine have been successfully tested in 3D cultures (23, 24), it is anticipated that culture conditions will be established to study mutation frequency at each dose and administration time point using 500–800 gene chip arrays.

[0045] Bey et al. in U.S. Pat. No. 5,614,557 discloses a compound of formula (III):

[0046] [ka]

[0047] [In the formula, (1) Y is FCH2-, F2CH-, or F3C-; (2)R a and R b are independently hydrogen, (C1-C4) alkylcarbonyl, or a group of formula

[0048] [ka]

[0049] A group represented by In formula (III(a)), R2 is hydrogen, (C1-C4) alkyl, benzyl, or p-hydroxybenzyl; (3) R1 is hydroxy, (C1-C8)alkoxy, -NR4R5, where R4 and R5 are independently hydrogen, (C1-C4)alkyl, or a group represented by formula (III(b)):

[0050] [ka]

[0051] A group represented by In formula (III(b)), R3 is hydrogen, C1-C4 alkyl, or p-hydroxybenzyl. The present invention discloses an analog of eflornithine represented by the formula:

[0052] U.S. Patent No. 5,002,879 to Bowlin et al. describes compounds of formula (IV) and (V): (IV); and

[0053] [ka]

[0054] and

[0055] [ka]

[0056] [In the formula, (1) X is -CHF2 or -CH2F, (2) R is hydrogen or -COR1, and (3) R1 is —OH or (C1-C6)alkoxy. Further ornithine decarboxylase inhibitors are disclosed, which are represented by the formula:

[0057] Water-soluble salts of eflornithine with polycations such as polycationic carbohydrates (chitosan, water-soluble chitosan derivatives, or salts thereof) or polycations such as polyamino acids, polyamines, polypeptides, basic polymers, or quaternary ammonium compounds are disclosed in U.S. Patent Application Publication No. 2002 / 0019338 by Hebert. All pharmaceutically acceptable salt forms, hydrates, and solvates of eflornithine, as well as its derivatives, analogs, and prodrugs, can be used in the methods and compositions of the present invention.

[0058] Further derivatives, analogs and prodrugs of eflornithine are known in the art. U.S. Patent Application Publication No. 2010 / 0120727 by Xu discloses conjugates in which a first moiety that is eflornithine or a derivative or analog of eflornithine is covalently attached to a second moiety that is a nonsteroidal anti-inflammatory drug (NSAID). NSAIDs include, for example, aspirin, aceclofenac, acemetacin, alclofenac, phenanthrene ... Phenac, amoxiprin, ampirone, azapropazone, benorylate, bromfenac, choline magnesium salicylate, choline salicylate, celecoxib, clofezone, diclofenac potassium, diclofenac sodium, diclofenac sodium with misoprostol, diflunisal, droxicam, lornoxicam, meloxicam, tenoxicam, ethenzamide, etodolac, fenoprofen calcium, faislamine, flurbiprofen, flufenamic acid, ibuprofen, ibuproxam, indoprofen, alminoprofen, carprofen, dexibuprofen, dexketoprofen, fenbufen, furo The steroid may be xaprofen, indomethacin, ketoprofen, ketorolac, kebuzone, loxoprofen, magnesium salicylate, meclofenamate sodium, metamizole, mefebutazone, oxyphenbutazone, phenazone, sulfinpyrazone, mefenamic acid, meloxicam, methyl salicylate, nabumetone, naproxen, naproxen sodium, nebumetone, oxaprozin, oxametacin, phenylbutazone, proglumetacin, piroxicam, pirprofen, suprofen, rofecoxib, salsalate, salicylsalicylate, salicylamide, sodium salicylate, sulindac, tiaprofenic acid, tolfenamic acid, tolmetin sodium, and valdecoxib. The first moiety and the second moiety can be linked via a covalent bond selected from the group consisting of an ester bond, an amide bond, an imine bond, a carbamate bond, a carbonate bond, a thioester bond, an acyloxycarbamate bond, an acyloxycarbonate bond, an acyloxythiocarbamate bond, a phosphate bond, a phosphoramidate bond, and an acyloxyphosphate bond.

[0059] U.S. Patent Application Publication No. 2015 / 0306241 by Zhu et al. discloses a copolymer of formula ABC or a pharmaceutically acceptable salt thereof, wherein A comprises a water-soluble polymer, B comprises a matrix metalloproteinase (MMP)-cleaving polypeptide, and C is a chemotherapeutic agent or derivative thereof, wherein A is linked to B at a first end through a first covalent bond or a first linking moiety and B is linked to C at a second end through a second covalent bond or a second linking moiety, and the copolymer is uncrosslinked. Typically, in this copolymer, the chemotherapeutic agent is an amino-containing therapeutic agent such as eflornithine.

[0060] US Patent Application Publication No. 2002 / 0110590 to Shaked et al. discloses a formulation for administering eflornithine comprising a core having immediate-release DFMO-containing granules and sustained-release granules and an outer layer surrounding the core comprising a pH-responsive coating.

[0061] U.S. Patent No. 9,034,319 to Teichberg et al. discloses the combined use of eflornithine with drugs that reduce blood glutamate levels and increase glutamate excretion from the brain to the blood. Drugs that reduce blood glutamate levels and increase glutamate excretion from the brain to the blood include: (1) glutamic oxaloacetic transaminase, glutamic pyruvate transaminase, acetylornithine transaminase, ornithine-oxoacid transaminase, succinyldiaminopimelic acid transaminase, 4-aminobutyric acid transaminase, (s)-3-amino-2-methylpropionic acid transaminase, 4-hydroxyglutamic acid transaminase, diiodotyrosine transaminase, thyroid hormone transaminase, tryptophan transaminase, diamine transaminase, and cysteine ​​transaminase. enzymes, L-lysine 6-transaminase, histidine transaminase, 2-aminoadipate transaminase, glycine transaminase, branched-chain amino acid transaminase, 5-aminovalerate transaminase, dihydroxyphenylalanine transaminase, tyrosine transaminase, phosphoserine transaminase, taurine transaminase, aromatic amino acid transaminase, aromatic amino acid glyoxylate transaminase, leucine transaminase, 2-aminohexanoate transaminase, ornithine (lysine) transaminase, kynurenine-oxoglutarate transaminase, D-4-his The enzyme may be a transaminase selected from the group consisting of hydroxyphenylglycine transaminase, cysteine-conjugate transaminase, 2,5-diaminovalerate transaminase, histidinol-phosphate transaminase, diaminobutyrate-2-oxoglutarate transaminase, and udp-2-acetamido-4-amino-2,4,6-trideoxyglucose transaminase; (2) glutamate dehydrogenase; (3) glutamate decarboxylase; (4) glutamate-ethylamine ligase; (5) transferase selected from the group consisting of glutamate N-acetyltransferase and adenylyltransferase; (6) aminomutase, which may be glutamate-1-semialdehyde 2,1-aminomutase; and (7) racemase. The enzyme may be used in combination with a cofactor.

[0062] US Patent No. 6,277,411 to Shaked et al. discloses preparations including capsules, tablets or other dosage forms containing different types of eflornithine cores.

[0063] Therefore, as will be described in further detail below, one aspect of the present invention is a method for treating glioma, comprising administering a therapeutically effective amount of eflornithine or its derivative or analog to a patient with glioma to reduce the mutation rate of glioma and thereby reduce the progression of glioma.Typically, the glioma is WHO grade II or grade III glioma.In one embodiment, the glioma is selected from the group consisting of anaplastic glioma, anaplastic oligodendroglioma, and mixed anaplastic oligoastrocytoma.

[0064] The above-mentioned eflornithine or its derivatives or analogs can be used together with other drugs. Pharmaceutically acceptable salt forms, hydrates and solvates of eflornithine and its derivatives, analogs and prodrugs can be used individually or in combination with other drugs.

[0065] US Patent No. 9,150,495 to Phanstiel IV discloses the use of eflornithine in combination with polyamine transporter selective compounds, including aromatic hydrocarbons disubstituted with polyamines.

[0066] US Patent No. 9,072,778 to Bachmann discloses the use of eflornithine in combination with SAM486A (S-adenosylmethionine decarboxylase inhibitor, 4-(aminoiminomethyl)-2,3-dihydro-1H-inden-1-one-diaminomethylenehydrazone), a verinoid and an anti-neoplastic agent.

[0067] US Patent No. 8,597,904 to Bachmann et al. discloses the use of eflornithine in combination with glidobactin, syringolin and other sylbactin compounds.

[0068] US Patent No. 7,718,764 to Wong et al. discloses conjugates of eflornithine with peptides, including VAPEEHPTLLTEAPLNPK (SEQ ID NO: 1) and fragments and derivatives thereof, for use as anti-neoplastic agents.

[0069] U.S. Patent No. 7,655,678 to Gupta et al. discloses the use of eflornithine in combination with celecoxib. U.S. Patent Application Publication No. 2003 / 0203956 to Masterrer discloses the use of eflornithine in combination with a cyclooxygenase-2 inhibitor selected from the group consisting of lumiracoxib, celecoxib, rofecoxib, etoricoxib, valdecoxib, parecoxib, and deracoxib. Similarly, U.S. Patent No. 6,258,845 to Gerner et al. discloses the use of eflornithine in combination with the nonsteroidal anti-inflammatory sulindac.

[0070] No. 7,432,302 to Burns et al. discloses the use of polyamine transport inhibitors in combination with eflornithine. Polyamine transport inhibitors have the structure RXL-polyamine, where R is a linear or branched C10 -C 50 saturated or unsaturated aliphatic, carboxyalkyl, carboalkoxyalkyl, or alkoxy; C1-C8 alicyclic moiety; mono- or polycyclic aryl-substituted or unsubstituted aliphatic; and aliphatic-substituted or unsubstituted mono- or polycyclic aromatic; mono- or polycyclic heterocyclic; mono- or polycyclic heteroaliphatic; arylsulfonyl; X is -CO-, -SO2-, or -CH2-; and L is a covalent bond or the naturally occurring amino acid lysine, ornithine, or 2,4-diaminobutyric acid.

[0071] U.S. Patent No. 7,425,579 to Poulin et al. discloses the use of polyamine transport inhibitors in combination with eflornithine. The polyamine transport inhibitors have the formula (PT-I) or (PT-II):

[0072] [ka]

[0073] ;

[0074] [ka]

[0075] wherein L is a linker, R1 is hydrogen, methyl, ethyl or propyl, R2 is hydrogen or methyl, and 0 <x<3;0<y<3;2<v<5;および2<w<8である) The compound may be a compound represented by the formula:

[0076] U.S. Patent No. 7,208,528 to Vermeulin et al. discloses the use of a polyamine transport inhibitor in combination with eflornithine. The polyamine transport inhibitor has the formula (PT-III):

[0077] [ka]

[0078] wherein R is selected from D or L amino acids; D or L ornithine, alicyclic, monocyclic or polycyclic aromatics; aliphatic-substituted monocyclic or polycyclic aromatics; and substituted or unsubstituted monocyclic or polycyclic heterocycles, and when R is a substituted monocyclic or polycyclic heterocycle, the heterocycle may be selected from OH, halogen, NO, NH, NH(CH), n CH3, N((CH2) n CH3)2, CN, (CH2) n CH3, O(CH2) n CH3, S(CH2) n CH3, NHCO(CH2) n CH3, or O(CF2) n CF3, COO(CH2) n CH3 (wherein n is 0 to 10) N shown by 1 -monosubstituted polyamine analogs or derivatives.

[0079] U.S. Patent No. 7,160,923 to Vermeulin et al. discloses the use of polyamine transport inhibitors in combination with eflornithine. The polyamine transport inhibitors may have the formula R1-X-R2, where R1-X- is represented by the formula R-NH-CR'R"-CO-, where NH-CR'R"-CO- is the D- or L-form of valine, asparagine, or glutamine, or the D-form of lysine or arginine, where R" is H, CH3, CH2CH3, or CHF2; where R is H or a straight-chain or branched C1-C 10 a head group selected from the group consisting of aliphatic, alicyclic, mono- or polycyclic aromatic, mono- or polycyclic aryl-substituted aliphatic, aliphatic-substituted mono- or polycyclic aromatic, mono- or polycyclic heterocyclic, mono- or polycyclic heterocyclic-substituted aliphatic, and aliphatic-substituted aromatic; and R2 is a polyamine.

[0080] U.S. Patent No. 7,144,920 to Burns et al. describes a compound of formula (PT-IV):

[0081] [ka]

[0082] wherein n can be 0-8, the aminomethyl functionality can be ortho-, meta-, or para-substituted, R is hydrogen, 2-aminoethyl, 3-aminopropyl, 4-aminobutyl, 5-aminopentyl, 6-aminohexyl, 7-aminoheptyl, or 8-aminooctyl, and R is hydrogen, and the polyamine is asymmetric. The present invention discloses polyamine analogs that induce antienzyme activity and inhibit polyamine transporter activity, including compounds of the formula:

[0083] U.S. Patent No. 7,094,808 to Bergeron, Jr. describes a compound of formula (PT-V):

[0084] [ka]

[0085] wherein R1 to R6 may be the same or different and are alkyl, aryl, arylalkyl or cycloalkyl (which may optionally have an alkyl chain interrupted by at least one ether oxygen atom), or hydrogen; 1 , N 2 , N 3 and N 4is a nitrogen atom capable of being protonated at physiological pH, a and b are integers from 1 to 4, which may be the same or different, and A, B, and C are bridging groups that effectively maintain a distance between the nitrogen atoms such that the polyamine: (i) is capable of being taken up by target cells upon administration of the polyamine to a human or non-human animal; and (ii) upon uptake by the target cells, the polyamine competitively binds to substantially the same biological counter anions as intracellular native polyamines in the target cells through electrostatic interactions between the positively charged nitrogen atoms; the administered polyamine, upon binding to biological counter anions in cells, functions biologically differently from intracellular polyamines and is a non-naturally occurring polyamine. The present invention discloses a polyamine transport inhibitor represented by the formula:

[0086] US Patent No. 7,030,126 to Ramesh et al. discloses the use of N(1),N(11)-diethylnorspermine (DENSPM), a polyamine analog, which can be used in combination with eflornithine as a polyamine synthesis inhibitor.

[0087] U.S. Patent No. 6,963,010 to Burns et al. discloses the use of hydrophobic polyamine analogs that can be used with eflornithine. These analogs include those represented by formulas (PT-VI), (PT-VII), (PT-VIII), and (PT-IX):

[0088] [ka]

[0089] ;

[0090] [ka]

[0091] ;

[0092] [ka]

[0093] ; and

[0094] [ka]

[0095] In the compound represented by formula (PT-VI), a, b, and c are independently 1 to 10. d and e are independently in the range of 0 to 30; each X is independently either a carbon (C) atom or a sulfur (S) atom, and R1 and R2 are independently selected from H or straight or branched C1-C 50 Saturated or unsaturated aliphatic, carboxyalkyl, carboalkoxyalkyl, or alkoxy; C1-C8 alicyclic; monocyclic or polycyclic aryl-substituted or unsubstituted aliphatic; aliphatic-substituted or unsubstituted monocyclic or polycyclic aromatic; monocyclic or polycyclic heterocyclic; monocyclic or polycyclic heterocyclic aliphatic; C1-C 10 alkyl; arylsulfonyl; or cyano; or R1X{O} n - and R2X{O} n Each - is independently replaced by H; * indicates a chiral carbon position, and if X is C, then n is 1; if X is S, then n is 2, and if X is C, then the XO group can be CH such that n is 0.

[0096] In the compound of formula (PT-VII), a, b, and c are independently in the range of 1 to 10, d and e are independently in the range of 0 to 30; R1, R2, R3, and R4 may be the same or different and are independently selected from H or linear or branched C1-C 50Saturated or unsaturated aliphatic, carboxyalkyl, carboalkoxyalkyl, or alkoxy; C1-C8 alicyclic; monocyclic or polycyclic aryl-substituted or unsubstituted aliphatic; aliphatic-substituted or unsubstituted monocyclic or polycyclic aromatic; monocyclic or polycyclic heterocyclic; monocyclic or polycyclic heterocyclic aliphatic; C1-C 10 It is selected from the group consisting of alkyl; arylsulfonyl; or cyano.

[0097] In the compound of formula (PT-VIII), a, b, and c are independently in the range of 1 to 10, d and e are independently in the range of 0 to 30; R1, R2, R3, and R4 may be the same or different and are independently selected from H or linear or branched C1-C 50 Saturated or unsaturated aliphatic, carboxyalkyl, carboalkoxyalkyl, or alkoxy; C1-C8 alicyclic; monocyclic or polycyclic aryl-substituted or unsubstituted aliphatic; aliphatic-substituted or unsubstituted monocyclic or polycyclic aromatic; monocyclic or polycyclic heterocyclic; monocyclic or polycyclic heterocyclic aliphatic; C1-C 10 It is selected from the group consisting of alkyl; arylsulfonyl; or cyano.

[0098] In the compounds of formula (PT-IX), a, b, and c are independently in the range of 1 to 10, and d and C are independently in the range of 0 to 30; Z1 is NRR and Z2 is selected from -R1, -CHR1R2, or -CR1R2R3, or Z2 is NRR and Z1 is selected from -R1, -CHR1R2, or -CR1R2R3, wherein R1, R2, and R3 may be the same or different and are independently selected from H, or a straight or branched C1-C 50 Saturated or unsaturated aliphatic, carboxyalkyl, carboalkoxyalkyl, or alkoxy; C1-C8 alicyclic; monocyclic or polycyclic aryl-substituted or unsubstituted aliphatic; aliphatic-substituted or unsubstituted monocyclic or polycyclic aromatic; monocyclic or polycyclic heterocyclic; monocyclic or polycyclic heterocyclic aliphatic; C1-C 10 It is selected from the group consisting of alkyl; arylsulfonyl; or cyano.

[0099] U.S. Patent No. 6,872,852 to Burns et al. discloses polyamine analogs that can be used with eflornithine, including compounds of the formula R1-X-R2, where R1 and R2 are independently H or straight-chain or branched C1-C 10 is a moiety selected from the group consisting of aliphatic, alicyclic, mono- or polycyclic aromatic, mono- or polycyclic aryl-substituted aliphatic, aliphatic-substituted mono- or polycyclic aromatic, mono- or polycyclic heterocyclic, mono- or polycyclic heterocyclic-substituted aliphatic and aliphatic-substituted aromatic, and halogenated forms thereof; X is a polyamine having two terminal amino groups, -(CH2)3-NH- or -CH2-Ph-CH2-.

[0100] U.S. Patent No. 6,646,149 to Vermeulen et al. describes a compound of the formula R1-X-R2, where , R1 and R2 are each a polyamine or a polyamine analog or derivative, and X is a linker moiety connecting the two polyamine moieties.

[0101] U.S. Patent No. 6,392,098 to Frydman et al. discloses conformationally locked polyamine analogs that can be used with eflornithine, including compounds of the formula E-NH-D-NH-BAB-NH-D-NH-E, where A is selected from the group consisting of C2-C6 alkenyl and C3-C6 cycloalkyl, cycloalkenyl, and cycloaryl; B is independently selected from the group consisting of a single bond and C1-C6 alkyl and alkenyl; D is independently selected from the group consisting of C1-C6 alkyl and alkenyl, and C3-C6 cycloalkyl, cycloalkenyl, and cycloaryl; and E is independently selected from the group consisting of H, C1-C6 alkyl and alkenyl.

[0102] U.S. Patent No. 6,083,496 to Poulin et al. discloses inhibitors of polyamine transport that can be used with eflornithine, including synthetic derivatives of dimers of the original polyamine, which have been modified to include an amide group bonded directly to a carbon atom of the original polyamine and positioned between two internal atoms, and the dimers are linked together by a spacer side chain anchored to the amide group of each monomer.

[0103] U.S. Patent No. 5,880,161 to Basu et al. describes a compound of the formula R1-NH-(CH2) w -NH-(CH2) x -NH-(CH2) y -NH-(CH2) z The present invention discloses polyamine analogs that can be used with eflornithine, including molecules having the formula -NH-R2, where R1 and R2 are hydrocarbon chains having 1 to 5 carbons, and w, x, y, and z are integers from 1 to 10; one preferred molecule is N 1 ,N 19 -bis-(ethylamino)-5,10,15-triazanonadecane.

[0104] US Patent No. 5,374,658 to Lau et al. discloses the use of oxidized polyamines, including N,N'-bis-(3-propionaldehyde)-1,4-diaminobutane (spermine bisaldehyde), in conjunction with eflornithine.

[0105] U.S. Patent No. 4,952,585 to Sunkara et al. discloses the use of eflornithine with esters of castanospermine. Similarly, U.S. Patent No. 4,792,558 to Sunkara et al. discloses the use of eflornithine with castanospermine.

[0106] US Patent No. 4,925,835 to Heston discloses aziridinylputrescine compounds such as 1-(4-aminobutyl)aziridine that can be used in conjunction with eflornithine.

[0107] US Patent No. 4,499,072 to Sunkara et al. discloses the use of eflornithine with interferon.

[0108] US Patent Application Publication No. 2010 / 0076009 to Towner et al. discloses the use of eflornithine with 2,4-disulfonylphenyl t-butyl nitrone (2,4-ds-PBN) in the treatment of glioma.

[0109] US Patent Application Publication No. 2015 / 0094336 by Zeldis describes 3-(4-amino-1-oxo-1,3-dihydro-isoindole-2-yl)- ... The use of eflornithine with thalidomide or thalidomide is disclosed.

[0110] U.S. Patent Application Publication No. 2010 / 0285012 by Dunn, Jr. et al. discloses the use of eflornithine with N-2-pyridinyl-2-pyridinecarbothioamide or cambendazole in the treatment of glioma.

[0111] U.S. Patent Application Publication No. 2013 / 0197088 by Casero, Jr. et al. discloses the use of eflornithine with inhibitors of histone demethylases, including oligoamines and polyamines, to treat malignant tumors.

[0112] US Patent Application Publication No. 2015 / 0050299 by Burns et al. discloses the use of eflornithine with one of several polyamine transport inhibitors, including AMXT1426, AMXT1501, AMXT1505, and AMXT1569.

[0113] US Patent Application Publication No. 2008 / 0027023 to Ellervik et al. discloses the use of eflornithine with aryl-substituted xylopyranoside derivatives.

[0114] U.S. Patent Application Publication No. 2015 / 0017231 to Phanstiel, IV et al. discloses the use of eflornithine with polyamine transport inhibitors having enhanced stability. The polyamine transport inhibitors have the structure R'HN-(CH2) x -NH-(CH2) y -NH-CH2-R-CH2-NH-(CH2) xx -NH-(CH2) yy -NHR" (wherein R is selected from the group consisting of anthracene, naphthalene, and benzene; R' and R" are independently selected from the group consisting of H and alkyl groups; and x, xx, y, and yy are independently selected from the group consisting of 3 and 4).

[0115] The above compounds may optionally be further substituted. In general, for optional substituents on saturated carbon atoms, such as those that are part of the structure of the above compounds, the following substituents may be used: C-C 10 aryl, heteroaryl containing 1 to 4 heteroatoms selected from N, O and S, C1-C 10 Alkyl, C1-C 10 Alkoxy, cycloalkyl, F, amino (NR 1 R 2 ), nitro, -SR, -S(O)R, -(S(O2)R, -S(O2)NR 1 R 2 , and -CONR 1 R 2 (These, in turn, may be optionally substituted.) Further descriptions of possible, optionally present substituents are provided below.

[0116] The above optional substituents included within the scope of the present invention do not substantially affect the activity of the derivatives or their stability, particularly their stability in aqueous solution. Definitions of some frequently occurring groups that can be used as optional substituents are provided below, but the omission of a group from these definitions should not be taken to mean that such a group cannot be used as an optional substituent, so long as the chemical and pharmacological requirements for the optional substituent are met.

[0117] As used herein, the term "alkyl" refers to an optionally substituted unbranched, branched, or cyclic saturated hydrocarbyl residue, or combinations thereof, having 1 to 12 carbon atoms, where the alkyl residue, when unsubstituted, contains only C and H. Typically, the unbranched or branched saturated hydrocarbyl residue has 1 to 6 carbon atoms, and is referred to herein as a "lower alkyl." When the alkyl residue is cyclic and contains a ring, the hydrocarbyl residue must contain at least three carbon atoms, the minimum number to form a ring. It goes without saying that the term "alkenyl" as used herein refers to an unbranched, branched, or cyclic hydrocarbyl residue having one or more carbon-carbon double bonds. As used herein, the term "alkynyl" refers to an unbranched, branched, or cyclic hydrocarbyl residue having one or more carbon-carbon triple bonds; the residue may also contain one or more double bonds. With respect to the use of "alkenyl" or "alkynyl," the presence of multiple double bonds does not necessarily result in an aromatic ring. As used herein, the terms "hydroxyalkyl," "hydroxyalkenyl," and "hydroxyalkynyl" refer to alkyl, alkenyl, or alkynyl groups, respectively, containing one or more hydroxyl groups as substituents; additional substituents may optionally be included, as detailed below. As used herein, the term "aryl" refers to a monocyclic or fused bicyclic moiety having the well-known characteristic of aromaticity; examples include phenyl, naphthyl, fluorenyl, and indenyl, which may optionally be substituted. As used herein, the term "hydroxyaryl" refers to an aryl group containing one or more hydroxyl groups as substituents; as further detailed below, additional substituents may optionally be included. As used herein, the term "heteroaryl" refers to a monocyclic or fused bicyclic ring system having the characteristic of aromaticity and containing one or more heteroatoms selected from O, S, and N. The inclusion of heteroatoms allows for aromaticity in five-membered rings as well as six-membered rings. Typical heteroaromatic systems include monocyclic C5-C6 heteroaromatic groups such as pyridyl, pyrimidyl, pyrazinyl, thienyl, furanyl, pyrrolyl, pyrazolyl, thiazolyl, oxazolyl, triazolyl, triazinyl, tetrazolyl, tetrazinyl and imidazolyl, as well as monocyclic C5-C6 heteroaromatic groups in which one of these monocyclic heteroaromatic groups is bonded to a phenyl ring or a C8-C6 heteroaromatic group. 10Fused bicyclic moieties formed by condensing with any of the heteroaromatic monocyclic groups to form a bicyclic group include indolyl, benzimidazolyl, indazolyl, benzotriazolyl, isoquinolyl, quinolyl, benzothiazolyl, benzofuranyl, pyrazolylpyridyl, quinazolinyl, quinoxalinyl, cinnolinyl, and other ring systems known to those skilled in the art. All monocyclic or fused-ring bicyclic systems characterized by aromaticity in terms of delocalized electron distribution throughout the ring system are encompassed by this definition. This definition also encompasses bicyclic groups in which at least the ring directly bonded to the rest of the molecule is characterized by aromaticity, including the delocalized electron distribution characteristic of aromaticity. Typically, the ring system contains 5 to 12 ring atoms and up to 4 heteroatoms, which are selected from the group consisting of N, O, and S. Monocyclic heteroaryls frequently contain 5-6 ring atoms and up to 3 heteroatoms selected from the group consisting of N, O, and S; bicyclic heteroaryls frequently contain 8-10 ring atoms and up to 4 heteroatoms selected from the group consisting of N, O, and S. The number and location of heteroatoms in a heteroaryl ring structure are subject to the well-known constraints of aromaticity and stability, which requires that the heteroaromatic group be sufficiently stable to exposure to water at physiological temperatures without rapid decomposition. As used herein, the term "hydroxyheteroaryl" refers to a heteroaryl group containing one or more hydroxyl groups as substituents; additional substituents may optionally be included, as further detailed below. As used herein, the terms "haloaryl" and "haloheteroaryl" refer to aryl and heteroaryl groups, respectively, substituted with at least one halo group, where "halo" refers to a halogen selected from the group consisting of fluorine, chlorine, bromine, and iodine; typically, the halogen is selected from the group consisting of chlorine, bromine, and iodine; and further substituents may optionally be included, as detailed below.As used herein, the terms "haloalkyl," "haloalkenyl," and "haloalkynyl" refer to alkyl, alkenyl, and alkynyl groups, respectively, that are substituted with at least one halo group, where "halo" refers to a halogen selected from the group consisting of fluorine, chlorine, bromine, and iodine; typically, the halogen is selected from the group consisting of chlorine, bromine, and iodine; as described in more detail below. Further substituents may optionally be included such that:

[0118] As used herein, the term "optionally substituted" indicates that the particular group or groups designated as optionally substituted may have no non-hydrogen substituents, or that the group or groups may have one or more non-hydrogen substituents consistent with the chemical and pharmacological activity of the resulting molecule. Unless otherwise specified, the total number of such substituents that may be present is equal to the total number of hydrogen atoms present in the unsubstituted form of the described group; fewer such substituents may be present than the maximum number. When an optional substituent, such as a carbonyl oxygen (C=O), is attached through a double bond, the group takes up two available valence electrons on the carbon atom to which the optional substituent is attached, thereby reducing the total number of substituents that may be included accordingly. As used herein, the term "substituted," whether used as part of "optionally substituted" or otherwise, when used to modify a particular group, moiety, or radical, means that one or more hydrogen atoms are replaced, each independently of the other, with one or more substituents, either the same or different.

[0119] Substituents useful for replacing saturated carbon atoms in the specified groups, moieties, or radicals include, but are not limited to, -Z a , =O, -OZ b , -SZ b , =S - , -NZ c Z c、=NZ b 、=N-OZ b 、トリハロメチル、-CF3、-CN、-OCN、-SCN、-NO、-NO2、=N2、-N3、-S(O)2Z b 、-S(O)2NZ b 、-S(O2)O - 、-S(O2)OZ b 、-OS(O2)OZ b 、-OS(O2)O - 、-OS(O2)OZ b 、-P(O)(O - )2、-P(O)(OZ b )(O - )、-P(O)(OZ b )(OZ b )、-C(O)Z b 、-C(S)Z b 、-C(NZ b )Z b 、-C(O)O - 、-C(O)OZ b 、-C(S)OZ b 、-C(O)NZ c Z c 、-C(NZ b )NZ c Z c 、-OC(O)Z b 、-OC(S)Z b 、-OC(O)O - 、-OC(O)OZ b 、-OC(S)OZ b 、-NZ b C(O)Z b 、-NZ b C(S)Z b 、-NZ b C(O)O - 、-NZ b C(O)OZ b 、-NZ b C(S)OZ b 、-NZ b C(O)NZ c Z c 、-NZ b C(NZ b )Z b 、-NZ b C(NZ b )NZ c Z cwhere Z a is selected from the group consisting of alkyl, cycloalkyl, heteroalkyl, cycloheteroalkyl, aryl, arylalkyl, heteroaryl, and heteroarylalkyl; and each Z b are independently hydrogen or Z a and each Z c is independently Z b or alternatively, two Z c can be taken together with the nitrogen atom to which they are attached to form a 4-, 5-, 6-, or 7-membered cycloheteroalkyl ring system, which may optionally contain 1 to 4 identical or different heteroatoms selected from the group consisting of N, O, and S. Specific examples include -NZ c Z c is intended to include, but is not limited to, -NH, -NH-alkyl, -N-pyrrolidinyl, and -N-morpholinyl, including other embodiments known in the art. Similarly, as another example, substituted alkyl is -alkylene-O-alkyl, -alkylene-heteroaryl, -alkylene-cycloheteroaryl, -alkylene-C(O)OZ, b , -Alkylene-C(O)NZ b Z b and -CH-CH-C(O)-CH, but are not limited to those specific forms and include other alternatives known in the art. One or more substituents, together with the atom to which they are attached, may form a cyclic structure, including, but not limited to, cycloalkyl and cycloheteroalkyl.

[0120] Similarly, useful substituents for replacing unsaturated carbon atoms in particular groups, moieties, or radicals include, but are not limited to, -Z a , halo, -O - , -OZ b , -SZ b , -S - , -NZ c Z c , trihalomethyl, -CF3, -CN, -OCN, -SCN, -NO, -NO2, -N3, -S(O)2Zb , -S(O2)O - , -S(O2)OZ b , -OS( O2)OZ b , -OS(O2)O - , -P(O)(O - )2, -P(O)(OZ b )(O - ), -P(O)(OZ b )(OZ b ), -C(O)Z b , -C(S)Z b , -C(NZ b )Z b , -C(O)O - , -C(O)OZ b , -C(S)OZ b , -C(O)NZ c Z c , -C(NZ b )NZ c Z c , -OC(O)Z b , -OC(S)Z b , -OC(O)O - , -OC(O)OZ b , -OC(S)OZ b , -NZ b C(O)OZ b , -NZ b C(S)OZ b , -NZ b C(O)NZ c Z c , -NZ b C(NZ b )Z b , and -NZ b C(NZ b )NZ c Z c where Z a , Z b and Z c has the meaning described above.

[0121] Similarly, useful substituents for substituting nitrogen atoms in heteroalkyl and cycloheteroalkyl groups include, but are not limited to, -Z a , halo, -O - , -OZ b , -SZb 、 -S - 、 -NZ c Z c 、 trihalomethyl, -CF3, -CN, -OCN, -SCN, -NO, -NO2, -S(O)2Z b 、 -S(O2)O - 、 -S(O2)OZ b 、 -OS(O2)OZ b 、 -OS(O2)O - 、 -P(O)(O - )2, -P(O)(OZ b )(O - )、 -P(O)(OZ b )(OZ b )、 -C(O)Z b 、 -C(S)Z b 、 -C(NZ b )Z b 、 -C(O)OZ<​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​The compounds described herein may have one or more chiral centers and / or double bonds and, therefore, may exist as stereoisomers, such as double bond isomers (i.e., geometric isomers such as E and Z), enantiomers, or diastereomers. The present invention encompasses isolated individual stereoisomeric forms (enantiomerically pure isomers, E and Z isomers, and other alternative forms for stereoisomers) as well as mixtures of stereoisomers, including racemic mixtures, diastereomeric mixtures, and mixtures of E and Z isomers with varying degrees of chiral purity or percentages of E and Z. Accordingly, the chemical structures depicted herein encompass all possible enantiomers and stereoisomers of the exemplified compounds, including stereomerically pure forms (e.g., geometrically pure, enantiomerically pure, or diastereomerically pure) and enantiomeric and stereoisomeric mixtures. Mixtures of enantiomers and stereoisomers can be resolved into their component enantiomers or stereoisomers using separation or chiral synthesis techniques well known to those skilled in the art. The present invention encompasses isolated individual stereoisomeric forms and mixtures of stereoisomers with varying chiral purities, including racemic mixtures. The present invention also encompasses various diastereomers. Other structures may be shown to depict specific isomers, but this is for convenience only and is not intended to limit the present invention to the depicted isomers. When a chemical name does not specify an isomeric form of a compound, the chemical name refers to any one of the possible isomeric forms or a mixture of isomeric forms of the compound. As noted above, eflornithine exists in two enantiomeric forms.

[0123] The present compound may also exist in several tautomeric forms, and when one tautomer is depicted herein, it is understood that it is merely for convenience and encompasses other tautomers of the depicted form.Therefore, the chemical structures depicted herein encompass all possible tautomeric forms of the illustrated compound.As used herein, the term "tautomer" refers to an isomer that is easily transformed into another, so that they can coexist in equilibrium; this equilibrium may be strongly favored by one of the tautomers, depending on stability considerations.For example, ketones and enols are two tautomeric forms of a compound.

[0124] As used herein, the term "solvate" refers to a compound formed by solvation (a combination of a solvent molecule with a solute molecule or ion), or an aggregate consisting of a solute ion or molecule, i.e., a compound of the present invention, and one or more solvent molecules. When water is the solvent, the corresponding solvate is a "hydrate." Examples of hydrates include, but are not limited to, hemihydrate, monohydrate, dihydrate, trihydrate, hexahydrate, and other water-containing species. Those skilled in the art will appreciate that pharmaceutically acceptable salts and / or prodrugs of the present compounds may also exist in solvated form. Solvates are typically formed by hydration, either as part of the preparation of the present compounds or through the natural absorption of water by the anhydrous compounds of the present invention.

[0125] As used herein, the term "ester" encompasses all esters of the present compounds in which any of the -COOH functional groups of the molecule is replaced with a -COOR functional group, where the R portion of the ester is any carbon-containing group that forms a stable ester moiety, including, but not limited to, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heterocyclyl, heterocyclylalkyl, and their substituted derivatives. Hydrolyzable esters of the present compounds are compounds in which the carboxyl group is present in the form of a hydrolyzable ester group. That is, these esters are pharmaceutically acceptable and can be hydrolyzed in vivo to the corresponding carboxylic acid.

[0126] In addition to the above substituents, the alkyl, alkenyl and alkynyl groups may alternatively or additionally be C1-C8 acyl, C2-C8 heteroacyl, C6-C 10 Aryl, C3-C8 cycloalkyl, C3-C8 heterocyclyl or C5-C 10 and optionally substituted heteroaryl (each of which may be optionally substituted). Furthermore, when two groups capable of forming a ring having 5 to 8 ring members are present on the same or adjacent atoms, the two groups may optionally be joined together with one or more atoms in the substituent to which they are attached to form such a ring.

[0127] "Heteroalkyl," "heteroalkenyl," and "heteroalkynyl," etc., are defined similarly to the corresponding hydrocarbyl (alkyl, alkenyl, and alkynyl) groups, except that the term "hetero" refers to groups containing one to three O, S, or N heteroatoms, or combinations thereof, within the backbone residue; at least one carbon atom of the corresponding alkyl, alkenyl, or alkynyl group is replaced with one of the specified heteroatoms to form the heteroalkyl, heteroalkenyl, or heteroalkynyl group, respectively. It will also be understood that for reasons of chemical stability, unless otherwise specified, the above groups will not contain more than two consecutive heteroatoms, except when an oxo group is present at N or S, as in a nitro or sulfonyl group.

[0128] As used herein, "alkyl" includes cycloalkyl and cycloalkylalkyl groups, although the term "cycloalkyl" can be used herein to describe carbocyclic non-aromatic groups linked via ring carbon atoms and "cycloalkylalkyl" can be used to describe carbocyclic non-aromatic groups linked to a molecule through an alkyl linker.

[0129] Similarly, "heterocyclyl" can be used to describe a non-aromatic cyclic group that contains at least one heteroatom (typically selected from N, O, and S) as a ring member and is linked to a molecule via a ring atom, which may be C (carbon-linked) or N (nitrogen-linked), and "heterocyclylalkyl" can be used to describe the above group linked to another molecule through a linker. A heterocyclyl can be fully saturated or partially saturated, but can be non-aromatic. Suitable sizes and substituents for cycloalkyl, cycloalkylalkyl, heterocyclyl, and heterocyclylalkyl groups include: The same as described above for alkyl groups. Heterocyclyl groups typically contain one, two, or three heteroatoms selected from N, O, and S as ring members, where N or S can be replaced by groups normally found at these atoms in heterocyclic ring systems. As used herein, these terms also encompass rings containing one or two double bonds, as long as the ring to which they are attached is not aromatic. Substituted cycloalkyl and heterocyclyl groups also include cycloalkyl or heterocyclyl rings fused with aromatic or heteroaromatic rings, provided that the attachment point of the group is to the cycloalkyl or heterocyclyl ring rather than to the aromatic / heteroaromatic ring.

[0130] As used herein, "acyl" encompasses groups containing an alkyl, alkenyl, alkynyl, aryl, or arylalkyl radical attached at one of the two available valence positions of the carbonyl carbon atom, and heteroacyl refers to corresponding groups in which at least one carbon other than the carbonyl carbon is replaced by a heteroatom selected from N, O, and S.

[0131] Acyl and heteroacyl groups are bonded to any group or molecule to which they are attached through the available valence electron of the carbonyl carbon atom. Typically, they are C1-C8 acyl groups, including formyl, acetyl, pivaloyl, and benzoyl, and C2-C8 heteroacyl groups, including methoxyacetyl, ethoxycarbonyl, and 4-pyridinoyl.

[0132] Similarly, "arylalkyl" and "heteroarylalkyl" refer to aromatic and heteroaromatic ring systems connected to their attachment points through a linking group, such as alkylene, including substituted or unsubstituted, saturated or unsaturated, cyclic or acyclic linkers. Typically, the linker is a C1-C8 alkyl. These linkers can also contain a carbonyl group, allowing them to provide substituents similar to acyl or heteroacyl moieties. The aryl or heteroaryl ring in an arylalkyl or heteroarylalkyl group can be substituted with the same substituents as described above for the aryl group. Preferably, the arylalkyl group contains a phenyl ring, optionally substituted with groups defined above for the aryl group, and a C1-C4 alkylene that is unsubstituted or substituted with one or two C1-C4 alkyl or heteroalkyl groups, which can optionally close to form a ring such as cyclopropane, dioxolane, or oxacyclopentane. Similarly, the heteroarylalkyl group preferably comprises a C5-C6 monocyclic heteroaryl group optionally substituted with groups described above as typical substituents for an aryl group and a C1-C4 alkylene that is unsubstituted or substituted with one or two C1-C4 alkyl or heteroalkyl groups, or an optionally substituted phenyl ring or a C5-C6 monocyclic heteroaryl and a C1-C4 heteroalkylene that is unsubstituted or substituted with one or two C1-C4 alkyl or heteroalkyl groups, in which case the alkyl or heteroalkyl group can optionally close to form a ring such as cyclopropane, dioxolane or oxacyclopentane.

[0133] When an arylalkyl group or a heteroarylalkyl group is described as being optionally substituted, the substituents may be present on the alkyl or heteroalkyl portion or the aryl or heteroaryl portion of the group. The optional substituents present on the alkyl or heteroalkyl portion are the same as those described above for alkyl groups generally; the optional substituents present on the aryl or heteroaryl portion are the same as those described above for aryl groups generally.

[0134] As used herein, "arylalkyl" groups, if they are unsubstituted, are hydrocarbyl groups and are described by the total number of carbon atoms in the ring and alkylene or similar linker, i.e., a benzyl group is a C7-arylalkyl group, and a phenylethyl is a C8-arylalkyl.

[0135] The above-mentioned "heteroarylalkyl" refers to a moiety containing an aryl group bonded through a linking group, and differs from "arylalkyl" in that at least one ring atom of the aryl moiety or one atom in the linking group is a heteroatom selected from N, O, and S. Heteroarylalkyl groups are described herein according to the total number of atoms in the ring and linker combined, and include aryl groups bonded through a heteroalkyl linker, heteroaryl groups bonded through a hydrocarbyl linker such as alkylene, and heteroaryl groups bonded through a heteroalkyl linker. Thus, for example, C7-heteroarylalkyl would include pyridylmethyl, phenoxy, and N-pyrrolylmethoxy.

[0136] As used herein, "alkylene" refers to a divalent hydrocarbyl group. Because alkylene is divalent, it can link two other groups together. Typically, alkylene is a -(CH) n- (where n is 1 to 8, preferably n is 1 to 4), however, where specified, alkylene may also be substituted with other groups and may be other lengths, and the open valence electrons need not be at opposite ends of the chain.

[0137] In general, any alkyl, alkenyl, alkynyl, acyl, or aryl or arylalkyl group contained in a substituent may itself optionally be substituted with further substituents, and unless otherwise described, the nature of the substituents is similar to that recited for the primary substituent itself.

[0138] As used herein, "amino" is designated -NH, however, when amino is described as "substituted" or "optionally substituted," this term encompasses NR'R" (where each R' and R" is independently H or an alkyl, alkenyl, alkynyl, acyl, aryl, or arylalkyl group, each of which may be optionally substituted with substituents described herein as suitable for the corresponding group); the R' and R" groups and the nitrogen atom to which they are attached form a 3- to 8-membered ring which may be saturated, unsaturated, or aromatic and contain 1-3 heteroatoms independently selected from N, O, and S as ring members, optionally substituted with substituents described as suitable for an alkyl group, or if NR'R" is an aromatic group, optionally substituted with substituents described as typical for a heteroaryl group.

[0139] As used herein, the terms "carbocycle," "carbocyclyl," or "carbocyclic" refer to a ring structure containing only carbon atoms in the ring, while the term "heterocycle" or "heterocyclic" refers to a ring containing heteroatoms. Carbocyclyls can be fully saturated or partially saturated, but can be non-aromatic. For example, carbocyclyl encompasses cycloalkyl. Carbocyclic and heterocyclic structures encompass compounds having monocyclic, bicyclic, or polycyclic ring systems; such systems can combine aromatic, heterocyclic, and carbocyclic rings. Mixed ring systems are described according to the ring that is attached to the remainder of the described compound.

[0140] As used herein, the term "heteroatom" includes any atom that is not carbon or hydrogen, such as nitrogen, oxygen, or sulfur. When part of the backbone or skeleton of a chain or ring, the heteroatom must be at least divalent and is typically selected from N, O, P, and and S.

[0141] As used herein, the term "alkanoyl" refers to an alkyl group covalently linked to a carbonyl (C=O) group. The term "lower alkanoyl" refers to an alkanoyl group in which the alkyl portion of the alkanoyl group is C1-C6. The alkyl portion of the alkanoyl group can be optionally substituted as described above. The term "alkylcarbonyl" can alternatively be used. Similarly, the terms "alkenylcarbonyl" and "alkynylcarbonyl" refer to an alkenyl or alkynyl group, respectively, linked to a carbonyl group.

[0142] As used herein, the term "alkoxy" refers to an alkyl group covalently bonded to an oxygen atom, which alkyl group may be considered as replacing the hydrogen atom of a hydroxyl group. The term "lower alkoxy" refers to an alkoxy group in which the alkyl portion of the alkoxy group is C1-C6. The alkyl portion of the alkoxy group may be optionally substituted as described above. As used herein, the term "haloalkoxy" refers to an alkoxy group in which the alkyl portion is substituted with one or more halo groups.

[0143] As used herein, the term "sulfo" refers to a sulfonic acid (-SO3H) substituent.

[0144] As used herein, the term "sulfamoyl" refers to a substituent having the structure -S(O2)NH2, in which the nitrogen of the NH2 portion of the group can be optionally substituted as described above.

[0145] As used herein, the term "carboxyl" refers to a group of the structure -C(O2)H.

[0146] As used herein, the term "carbamyl" refers to a group of the structure -C(O2)NH2, in which the nitrogen of the NH2 portion of the group can be optionally substituted as described above.

[0147] As used herein, the terms "monoalkylaminoalkyl" and "dialkylaminoalkyl" refer to groups of the structure -Alk1-NH-Alk2 and -Alk1-N(Alk2)(Alk3), where Alk1, Alk2 and Alk3 refer to alkyl groups as defined above.

[0148] As used herein, the term "alkylsulfonyl" refers to a group of the structure -S(O)2-Alk, where Alk is an alkyl group as defined above. The terms "alkenylsulfonyl" and "alkynylsulfonyl" similarly refer to a sulfonyl group covalently bonded to an alkenyl group and an alkynyl group, respectively. The term "arylsulfonyl" refers to a group of the structure -S(O)2-Ar, where Ar is an aryl group as defined above. The term "aryloxyalkynylsulfonyl" refers to a group of the structure -S(O)2-Alk-O-Ar, where Alk is an alkyl group as defined above and Ar is an aryl group as defined above. The term "arylalkylsulfonyl" refers to a group of the structure -S(O)2-AlkAr, where Alk is an alkyl group as defined above and Ar is an aryl group as defined above.

[0149] As used herein, the term "alkyloxycarbonyl" refers to an ester substituent containing an alkyl group, where the carbonyl carbon is the point of attachment to the molecule. An example is ethoxycarbonyl, which is CH3CH2OC(O)-. Similarly, the terms "alkenyloxycarbonyl," "alkynyloxycarbonyl," and "cycloalkylcarbonyl" refer to similar ester substituents containing an alkenyl group, an alkenyl group, or a cycloalkyl group, respectively. Similarly, the term "aryloxycarbonyl" refers to an ester substituent containing an aryl group. "aryloxyalkylcarbonyl" refers to an ester substituent where the carbonyl carbon is the point of attachment to the molecule. Similarly, the term "aryloxyalkylcarbonyl" refers to an ester substituent containing an alkyl group where the alkyl group is itself substituted with an aryloxy group.

[0150] Other combinations of substituents are known in the art and are described, for example, in U.S. Patent No. 8,344,162 to Jung et al., which is incorporated herein by reference. For example, the term "thiocarbonyl" and substituent combinations containing "thiocarbonyl" include carbonyl groups in which a double-bonded sulfur replaces the normal double-bonded oxygen in the group. The term "alkylidene" and similar terms are intended to encompass alkyl, alkenyl, alkynyl, or cycloalkyl groups, as specified, in which two hydrogen atoms have been removed from a single carbon atom, said group being double-bonded to the remainder of the structure.

[0151] The compounds disclosed herein can exist as salts in physiological pH ranges or other ranges. Such salts are further described below. In general, the term "pharmaceutically acceptable salts" is intended to encompass salts of active compounds prepared with relatively non-toxic acids or bases, depending on the specific substituents found in the compounds described herein. When the compounds of the present invention contain relatively acidic functional groups, base addition salts can be obtained by contacting the neutral form of such compounds, neat or in a suitable inert solvent, with a sufficient amount of the desired base. Examples of pharmaceutically acceptable base addition salts include sodium, potassium, calcium, ammonium, organic amino or magnesium salts, or similar salts. When a compound of the present invention contains a relatively basic functional group, an acid addition salt can be obtained by contacting the neutral form of such compound, either neat or in a suitable inert solvent, with a sufficient amount of the desired acid. Examples of pharmaceutically acceptable acid addition salts include salts derived from inorganic acids such as hydrochloric acid, hydrobromic acid, nitric acid, carbonic acid, monohydrogencarbonic acid, phosphoric acid, monohydrogenphosphate, dihydrogenphosphate, sulfuric acid, monohydrogensulfuric acid, hydroiodic acid, or phosphorous acid, as well as salts derived from relatively non-toxic organic acids such as acetic acid, propionic acid, isobutyric acid, oxalic acid, maleic acid, malonic acid, benzoic acid, succinic acid, suberic acid, fumaric acid, mandelic acid, phthalic acid, benzenesulfonic acid, p-tolylsulfonic acid, citric acid, tartaric acid, methanesulfonic acid, and the like. Also included are salts of amino acids such as arginate, and salts of organic acids such as glucuronic acid and galacturonic acid (see, e.g., Berge, S.M. et al., "Pharmaceutical Salts," Journal of Pharmaceutical Science, 1977, 66, 1-19). Certain specific compounds of the present invention contain both basic and acidic functional groups that allow the compounds to be converted into either base or acid addition salts.

[0152] Therefore, one aspect of the present invention is a method for treating glioma, comprising administering a therapeutically effective amount of eflornithine or a derivative or analog thereof to treat glioma by inhibiting the progression of DNA mutations induced by chemotherapy agents to reduce the progression or malignancy of glioma. Pharmaceutically acceptable salt forms, hydrates and solvates of eflornithine, as well as its derivatives, analogs and prodrugs, can all be used in this method.

[0153] Typically, the glioma has been previously treated with radiation therapy and adjuvant alkylating agent therapy and is a recurrent / refractory anaplastic glioma. The glioma may have a mutation in one or more genes selected from the group consisting of IDH1, IDH2, TP53, PTEN, and ATRX. The glioma may have a methylated MGMT promoter.

[0154] Eflornithine or a derivative or analog thereof may be administered alone or in combination with a therapeutically effective amount of one or more conventional antineoplastic agents used in the treatment of gliomas. The therapeutic agents may include, but are not limited to, alkylating agents, antimetabolites, angiogenesis inhibitors, EGFR inhibitors, platinum-containing agents, topoisomerase inhibitors, or other classes of agents. For example, but not limited to, these agents include lomustine (CCNU), carmustine (BCNU), temozolomide, procarbazine, prednisone, vincristine, PCV (a combination of lomustine, procarbazine, and vincristine), carboplatin, carboplatin plus thymidine, carmustine plus temozolomide, erlotinib, carboplatin plus erlotinib, chloretazine, lomustine plus chloretazine, imatinib, hydroxyurea, hydroxyurea plus imatinib, irinotecan, thalidomide, temozolomide plus thalidomide, rilotumumab, cilengitide, cisplatin ... These drugs may include retinoic acid, celecoxib, cis-retinoic acid + celecoxib, enzastaurin, sirolimus, erlotinib + sirolimus, fenretinide, gefitinib, lapatinib, temsirolimus, tipifarnib, vorinostat, diaziquone, methotrexate, melphalan, a combination of vincristine, prednisone, and procarbazine, thioguanine, TPDCV (thioguanine, procarbazine, dibromodulcitol, lomustine, vincristine), a combination of nitrogen mustard, vincristine, and procarbazine, tenoposide, and carboplatin + tenoposide. Other drugs and drug combinations are known in the art.

[0155] In one embodiment, eflornithine or a derivative or analog thereof reduces the mutation rate of gliomas in conjunction with the administration of an alkylating agent, such as, but not limited to, temozolomide.

[0156] Eflornithine or a derivative or analog thereof can also be used in combination with radiation therapy, either alone or together with one or more of the additional agents listed above.

[0157] Additionally, eflornithine or a derivative or analog thereof can be used in combination with the polyamine transport inhibitors or polyamine analogs described above, either alone or together with one or more additional agents described above.

[0158] Additionally, eflornithine or a derivative or analog thereof can be used in combination with an S-adenosylmethionine decarboxylase inhibitor, either alone or together with one or more additional agents as described above.

[0159] Eflornithine or its derivatives or analogs may also be used in combination with, but are not limited to, (1) retinoids, (2) glidobactin, syringolin, and other silbactin compounds, (3) celecoxib and other cyclooxygenase-2 inhibitors, (4) verinoids, (5) nonsteroidal anti-inflammatory drugs such as sulindac, (6) castanospermine and castanospermine esters, (7) aziridinylputrescine compounds such as 1-(4-aminobutyl)aziridine, (8) interferons, (9) aryl-substituted xylopyranoside derivatives, (10) benzodiazepines, (11) benzodiazepines, (12) benzodiazepines, (13) benzodiazepines, (14) benzodiazepines, (15) benzodiazepines, (16) benzodiazepines, (17) benzodiazepines, (18) benzodiazepines, (19) benzodiazepines, (20) benzodiazepines, (21) benzodiazepines, (22) benzodiazepines, (23) benzodiazepines, (24) benzodiazepines, (25) benzodiazepines, (26) benzodiazepines, (27) benzodiazepines, (28) benzodiazepines, (29) benzodiazepines, (30) benzodiazepines, (31) benzodiazepines, (32) benzodiazepines, (33) benzodiazepines, (34) benzodiazepines, (35) benzodiazepines, (36) benzodiazepines, (37) benzodiazepines, (38) benzodiazepines, (39) benzodiazepines, (40) benzodiaze ) agents that reduce blood glutamate levels and increase glutamate excretion from the brain to the blood, (10) chitosan and chitosan derivatives and analogs, (11) 2,4-disulfonylphenyl t-butyl nitrone, (12) 3-(4-amino-1-oxo-1,3-dihydro-isoindol-2-yl)-piperidine-2,6-dione, (13) thalidomide, (14) N-2-pyridinyl-2-pyridinecarbothioamide, (15) cambendazole, and (16) histone demethylase inhibitors.

[0160] In another embodiment, eflornithine or a derivative or analog thereof is administered with an agent that enhances the ability of eflornithine or a derivative or analog thereof to cross the blood-brain barrier. Typically, eflornithine or a derivative or analog thereof is administered with an agent that enhances the ability of eflornithine or a derivative or analog thereof to cross the blood-brain barrier. Drugs that enhance the ability to cross the barrier include: (a) Formula (D-III):

[0161] [ka]

[0162] wherein (A) A is somatostatin, thyrotropin-releasing hormone (TRH), vasopressin, alpha interferon, endorphin, muramyl dipeptide, or an ACTH4-9 analog, and (B) B is insulin, IGF-I, IGF-II, transferrin, cationized (basic) albumin, or prolactin. or a chimeric peptide of formula (D-III) [wherein the disulfide-covalent bridge between A and B is represented by the sub-formula (D-III(a)):

[0163] [ka]

[0164] where the crosslinks are formed using cysteamine and EDAC as crosslinking reagents. or a chimeric peptide of the structure represented by formula (D-III) [wherein the disulfide-covalent bridge between A and B is substituted by a bridge represented by the sub-formula (D-III(b)):

[0165] [ka]

[0166] where the crosslinks are formed using glutaraldehyde as the crosslinking reagent. a chimeric peptide having the structure shown in (b) a composition comprising avidin or an avidin fusion protein bound to biotinylated eflornithine or an analog or derivative thereof, wherein an avidin-biotin drug complex is formed therein with a protein selected from the group consisting of insulin, transferrin, anti-receptor monoclonal antibodies, cationized proteins, and lectins; (c) a neutral liposome that is pegylated and incorporates eflornithine or an analog or derivative thereof, wherein the polyethylene glycol chain is conjugated to at least one transportable peptide or targeting agent; (d) a humanized murine antibody that binds to the human insulin receptor bound to eflornithine or an analog or derivative thereof through an avidin-biotin bond; and (e) the first segment and the second segment, i.e., after binding to the variable region of the antibody a first segment comprising a variable region of an antibody that recognizes an antigen on the surface of a cell that undergoes antibody-receptor-mediated endocytosis, and optionally may further comprise at least one domain of the constant region of the antibody; and a second segment comprising a protein domain selected from the group consisting of avidin, avidin muteins, chemically modified avidin derivatives, streptavidin, streptavidin muteins, and chemically modified streptavidin derivatives, wherein the fusion protein is bound to eflornithine or an analog or derivative thereof by a covalent bond to biotin. The agent is selected from the group consisting of:

[0167] When administering multiple therapeutic agents, each therapeutic agent can be administered separately, or two or more therapeutic agents can be administered in a single pharmaceutical composition. For example, when administering three therapeutic agents, the following possibilities exist: (1) The three therapeutic agents are each administered individually; in this case, each agent can be administered in a separate pharmaceutical composition or as a single agent without a pharmaceutical composition for the agent. Further details regarding the composition and preparation of pharmaceutical compositions are provided below. In such embodiments, zero, one, two, or three different pharmaceutical compositions can be used. (2) Two of the therapeutic agents are administered together in a single pharmaceutical composition, and the third therapeutic agent is administered separately, either as a single agent or as a separate pharmaceutical composition. (3) All three therapeutic agents are administered together in a single pharmaceutical composition.

[0168] Another aspect of the present invention is a method for producing a semiconductor device comprising: (1) a therapeutically effective amount of eflornithine or an eflornithine derivative or analog as described above; (2) optionally, a therapeutically effective amount of at least one additional agent as described above that can be used in conjunction with eflornithine or an eflornithine derivative or analog; and (3) Pharmaceutically acceptable carrier A pharmaceutical composition for treating glioma, comprising:

[0169] Conventional pharmaceutically acceptable carriers are known in the art and include, but are not limited to, sugars, solvents, thickeners, emulsifiers, diluents, sweeteners, humectants, organic acids, colorants, flavors, and preservatives.

[0170] In one embodiment, the composition according to the present invention can further comprise an agent that enhances the ability of eflornithine or a derivative or analog thereof to cross the blood-brain barrier. Typically, the agent that enhances the ability of eflornithine or a derivative or analog thereof to cross the blood-brain barrier is: (a) Formula (D-III):

[0171] [ka]

[0172] wherein (A) A is somatostatin, thyrotropin-releasing hormone (TRH), vasopressin, alpha interferon, endorphin, muramyl dipeptide, or an ACTH4-9 analog, and (B) B is insulin, IGF-I, IGF-II, transferrin, cationized (basic) albumin, or prolactin. or a chimeric peptide of formula (D-III) [wherein the disulfide-covalent bridge between A and B is represented by the sub-formula (D-III(a)):

[0173] [ka]

[0174] where the crosslinks are formed using cysteamine and EDAC as crosslinking reagents. or a chimeric peptide of the structure represented by formula (D-III) [wherein the disulfide-covalent bridge between A and B is substituted by a bridge represented by the sub-formula (D-III(b)):

[0175] [ka]

[0176] where the crosslinks are formed using glutaraldehyde as the crosslinking reagent. a chimeric peptide having the structure shown in (b) a composition comprising avidin or an avidin fusion protein bound to biotinylated eflornithine or an analog or derivative thereof, wherein an avidin-biotin drug complex is formed therein with a protein selected from the group consisting of insulin, transferrin, anti-receptor monoclonal antibodies, cationized proteins, and lectins; (c) a neutral liposome that is pegylated and incorporates eflornithine or an analog or derivative thereof, wherein the polyethylene glycol chain is conjugated to at least one transportable peptide or targeting agent; (d) a humanized murine antibody that binds to the human insulin receptor bound to eflornithine or an analog or derivative thereof through an avidin-biotin bond; and (e) A fusion protein comprising a first segment and a second segment, i.e., a first segment comprising an antibody variable region that recognizes an antigen on the surface of a cell that undergoes antibody-receptor-mediated endocytosis after binding to the antibody variable region, and optionally further comprising at least one domain of the antibody constant region; and a second segment comprising a protein domain selected from the group consisting of avidin, avidin muteins, chemically modified avidin derivatives, streptavidin, streptavidin muteins, and chemically modified streptavidin derivatives, wherein the fusion protein is bound to eflornithine or an analog or derivative thereof by a covalent bond to biotin. The agent is selected from the group consisting of:

[0177] In one embodiment, the pharmaceutical composition according to the present invention may contain a prodrug. When the pharmaceutical composition according to the present invention contains a prodrug, the prodrug and active metabolite of the compound can be identified using conventional techniques known in the art. For example, see Bertolini et al., J. Med. Chem., 40, 2011-2016 (1997); Shan et al., J.Pharm.Sci.,86(7),765-767;Bagshawe,Drug Dev.Res.,34,220-230(1995);Bodor,Advances in Drug Res.,13,224-331(1984);Bundgaard,Design of Prodrugs(Elsevier Pr ess 1985); Larsen, Design and Application of Prodrugs, Drug Design and Development (Krogsgaard-Larsen et al., eds., Harwood Academic Publishers, 1991); Dear et al., J. Chromatogr. B, 748, 281-293 (2000); Spraul et al., J. Pharmaceutical & Biomedical Analysis, 10, 601-605 (1992); and Prox et al., Xenobiol., 3, 103-112 (1992).

[0178] When the pharmacologically active compound in the pharmaceutical composition according to the present invention has a sufficiently acidic functional group, a sufficiently basic functional group, or both a sufficiently acidic and a sufficiently basic functional group, these groups can react accordingly with a number of inorganic or organic bases, and inorganic and organic acids, to form pharmaceutically acceptable salts. Representative pharmaceutically acceptable salts include salts prepared by reaction of the pharmacologically active compound with an inorganic or organic acid or inorganic base, such as sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, phosphate, monohydrogen phosphate, dihydrogen phosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, decanoate, caprylate, acrylate, formate, isobutyrate, caproate, heptanoate, propiolate, oxalate, malonate, succinate, suberate, sebacate, fumarate, and maleate. , butyne-1,4-dionate, hexyne-1,6-dionate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, phthalate, sulfonate, xylenesulfonate, phenylacetate, phenylpropionate, phenylbutyrate, citrate, lactate, β-hydroxybutyrate, glycolate, tartrate, methanesulfonate, propanesulfonate, naphthalene-1-sulfonate, naphthalene-2-sulfonate, and mandelate salts. When a pharmacologically active compound possesses one or more basic functional groups, the desired pharmaceutically acceptable salt may be prepared by any suitable method available in the art, for example, by treatment of the free base with an inorganic acid such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or an organic acid such as acetic acid, maleic acid, succinic acid, mandelic acid, fumaric acid, malonic acid, pyruvic acid, oxalic acid, glycolic acid, salicylic acid, and the like; a pyranosidyl acid such as glucuronic acid or galacturonic acid; an alpha-hydroxy acid such as citric acid or tartaric acid; an amino acid such as aspartic acid or glutamic acid; an aromatic acid such as benzoic acid or succinic acid; a sulfonic acid such as p-toluenesulfonic acid or ethanesulfonic acid, and the like.When a pharmacologically active compound possesses one or more acidic functional groups, the desired pharmaceutically acceptable salt can be prepared by any suitable method available in the art, for example, treatment of the free acid with an inorganic or organic base, such as an amine (primary, secondary, or tertiary), an alkali metal hydroxide, or an alkaline earth metal hydroxide. Representative examples of suitable salts include organic salts derived from amino acids such as glycine and arginine, ammonia, primary, secondary, and tertiary amines, and cyclic amines such as piperidine, morpholine, and piperazine, as well as inorganic salts derived from sodium, calcium, potassium, magnesium, manganese, iron, copper, zinc, aluminum, and lithium.

[0179] For pharmaceutical agents that are solid, it will be understood by those skilled in the art that the compounds and salts of the present invention may exist in various crystalline or polymorphic forms, all of which are intended to be included within the scope of the present invention and the specified formula.

[0180] The plasma concentration in a subject may be about 60 μM to about 1000 μM. In some embodiments, the plasma concentration may be about 200 μM to about 800 μM. In other embodiments, the concentration may be about 300 μM to about 600 μM. In still other embodiments, the plasma concentration may be about 400 to about 800 μM. In another embodiment, the plasma concentration may be about 0.5 μM to about 20 μM, typically 1 μM to about 10 μM.

[0181] The compositions of the present invention can be manufactured using known techniques for preparing pharmaceutical compositions, for example, by conventional techniques such as mixing, dissolving, granulating, dragee-making, levitating, emulsifying, encapsulating, entrapping, or lyophilizing. Pharmaceutical compositions can be formulated in a conventional manner using one or more physiologically acceptable carriers, which can be selected from excipients and auxiliaries that can be used pharmaceutically and that facilitate the formulation of active compounds.

[0182] The appropriate formulation will vary depending on the selected route of administration. For injection, the agent of the present invention can be formulated in aqueous solution, preferably in a physiologically compatible buffer such as Hanks' solution, Ringer's solution, or physiological saline buffer. For transmucosal administration, a penetrant appropriate to the barrier to be permeated is used in the formulation. Such penetrants are known in the art.

[0183] For oral administration, compounds can be easily formulated by combining the active compound with pharmaceutically acceptable carriers known in the art. These carriers allow the compounds of the present invention to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, solutions, suspensions, and the like, for oral ingestion by the patient to be treated. Oral pharmaceutical preparations are obtained by mixing a solid excipient with the active ingredient (drug), optionally grinding the resulting mixture, and, if desired, processing the granular mixture into tablets or dragee cores after adding suitable excipients. Suitable excipients include fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; and cellulose preparations, such as corn starch, wheat starch, rice starch, potato starch, gum, methylcellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, or polyvinylpyrrolidone (PVP). If desired, disintegrating agents may be added, such as cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate.

[0184] The dragee core is provided with a suitable coating.For this purpose, concentrated sugar solutions can be used, which may optionally contain gum arabic, polyvinylpyrrolidone, carbopol gel, polyethylene glycol, and / or titanium dioxide, lacquer liquid, and suitable organic solvents or solvent mixtures.Dyes or pigments can be added to the tablet or dragee coating for identification or to indicate the characteristics of different combinations of active agents.

[0185] Orally available pharmaceutical preparations include push-fit capsules made of gelatin and soft, sealed capsules made of gelatin and a plasticizer such as glycerin or sorbitol. Push-fit capsules can contain the active ingredient mixed with a filler such as lactose, a binder such as starch, and / or a lubricant such as talc or magnesium stearate, and optionally, stabilizers. In soft capsules, the active ingredient can be dissolved or suspended in a suitable liquid such as fatty oils, liquid paraffin, or liquid polyethylene glycol. Additionally, stabilizers may be added. All oral preparations should be in a dosage form suitable for oral administration. For buccal administration, the composition can be in the form of tablets or lozenges formulated in a conventional manner.

[0186] Pharmaceutical preparations for parenteral administration can include aqueous solutions or suspensions. Suitable lipophilic solvents or bases include fatty oils such as sesame oil or synthetic fatty acid esters such as ethyl oleate or triglycerides. Aqueous suspensions for injection can contain substances that increase the viscosity of the suspension, such as sodium carboxymethylcellulose, sorbitol, or dextran. In some cases, the suspension can also contain suitable stabilizers or modifiers that increase the solubility or dispersibility of the composition, allowing the preparation of highly concentrated solutions, or can contain suspending or dispersing agents. Oral pharmaceutical preparations can combine a pharmacologically active agent with a solid excipient, The resulting mixture is optionally milled, and if desired, the granular mixture is processed into tablets or dragee cores after adding suitable auxiliaries. Suitable excipients include, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as corn starch, wheat starch, rice starch, potato starch, gelatin, tragacanth gum, methylcellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and / or polyvinylpyrrolidone (PVP). If desired, disintegration regulators such as cross-linked polyvinylpyrrolidone, agar, or alginic acid or its salts, such as sodium alginate, may also be added.

[0187] Other ingredients may be used, such as stabilizers, e.g., sodium citrate, ascorbyl palmitate, propyl gallate, reducing agents, antioxidants such as ascorbic acid, vitamin E, sodium bisulfite, butylated hydroxytoluene, BHA, acetylcysteine, monothioglycerol, phenyl-α-naphthylamine, or lecithin. Chelating agents such as EDTA may also be used. Other ingredients conventional in the field of pharmaceutical compositions and formulations, such as lubricants, colorants, and flavorings in tablets or pills, may also be used. Conventional pharmaceutical excipients or carriers may also be used. Pharmaceutical excipients may include, but are not limited to, calcium carbonate, calcium phosphate, various sugars or types of starch, cellulose derivatives, gelatin, vegetable oils, polyethylene glycol, and physiologically compatible solvents. Other pharmaceutical excipients are also well known in the art. Representative pharmaceutically acceptable carriers include any and all solvents, including, but not limited to, aqueous and non-aqueous solvents, dispersion media, coating solutions, antibacterial and / or antifungal agents, isotonic and / or absorption delaying agents, etc. The use of such media and / or agents for pharmaceutical active substances is well known in the art. Except insofar as any conventional media, carrier, or agent is incompatible with the active ingredient(s), its use in the compositions of the present invention is contemplated. Supplementary active ingredients can also be incorporated into the compositions, particularly as described above. For administration of any of the compounds used in the present invention, preparations should meet sterility, pyrogenicity, general safety, and purity standards as required by the FDA Office of Biologics Standards or other regulatory agencies governing drugs.

[0188] For intranasal or inhalation administration, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray from pressurized packs or nebulizers, using a suitable propellant, for example, dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol, the dosage unit can be determined by providing a valve to deliver a metered amount. Gelatin capsules and cartridges for use in inhalers or insufflators can be formulated containing a powder mix of the compound and a suitable powder base, such as lactose or starch.

[0189] The compound can be formulated for parenteral administration by injection, for example, by bolus injection or continuous infusion.The preparation for injection can be in the form of a unit dose, for example, in ampoules or multi-dose containers, with preservatives added.The composition can be in the form of a suspension, solution or emulsion in oily or aqueous base, and can contain formulating agents such as suspending agents, stabilizers and / or dispersing agents.

[0190] Pharmaceutical preparations for parenteral administration include aqueous solutions of the active compound in water-soluble form. Additionally, suspensions of the active agent may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or bases include fatty oils such as sesame oil, or synthetic fatty acid esters such as ethyl oleate or triglycerides, or liposomes. Aqueous injection suspensions may contain substances that increase the viscosity of the suspension, such as sodium carboxymethylcellulose, sorbitol, or dextran. Optionally, the suspension may also contain suitable stabilizers or agents that increase the solubility of the compounds to allow for the preparation of highly concentrated solutions.

[0191] Alternatively, the active ingredient may be in powder form for constitution with a suitable base, e.g., sterile pyrogen-free water, before use. The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, e.g., containing conventional suppository bases such as cocoa butter or other glycerides.

[0192] In addition to the above-mentioned formulations, the compound can also be formulated as depot preparations.The above-mentioned long-acting preparations can be administered by implantation (for example, subcutaneously or intramuscularly) or intramuscular injection.That is, for example, the compound can be formulated with suitable polymeric or hydrophobic materials (for example, as an emulsion in an acceptable oil) or ion exchange resin, or as a sparingly soluble derivative, for example, as a sparingly soluble salt.

[0193] The pharmaceutical compositions may also comprise suitable solid- or gel-phase carriers or excipients, examples of which include calcium carbonate, calcium phosphate, sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols.

[0194] Pharmaceutical compositions can be administered by various methods known in the art. The route and / or mode of administration varies depending on the desired results. Depending on the route of administration, the pharmacologically active agent(s) may be coated with a material that protects the therapeutic agent(s) from the action of acids and other compounds that may inactivate the agent. Conventional pharmaceutical practice can be employed to provide suitable formulations or compositions for administering the pharmaceutical composition to a subject. Any appropriate route of administration can be employed, including, but not limited to, intravenous, parenteral, intraperitoneal, intravenous, transdermal, subcutaneous, intramuscular, or oral administration. Depending on the severity of the malignancy or other disease, disorder, or condition being treated, as well as other conditions affecting the subject being treated, either systemic or local delivery of the pharmaceutical composition can be used during the course of treatment. The pharmaceutical composition can be administered together with additional therapeutic agents intended to treat a particular disease or condition, which may be the same disease or condition intended to be treated with the pharmaceutical composition, a related disease or condition, or even an unrelated disease or condition.

[0195] As detailed above, eflornithine and its derivatives or analogs have been reported to be effective in treating glioma, particularly in inhibiting or delaying the progression of glioma to more aggressive pathologies.However, since all forms of cancer are associated with mutations in malignant tumor cells, eflornithine or its derivatives or analogs can also be administered to inhibit or delay the progression of other malignant tumors by blocking mutations in malignant tumor cells.Eflornithine or its derivatives or analogs can be used to slow the progression of many types of cancer and block mutations in many types of cancer, and in particular, eflornithine or its derivatives or analogs can be used to treat neuroblastoma.Eflornithine or its derivatives or analogs increase the concentration of p21(waf1 / cip1) and p27kip-1, which acts as a trigger for cell cycle arrest.Tumor types to which the above findings apply include leukemia, pancreatic cancer, neuroblastoma, breast cancer and gastric cancer. The above is discussed in the following references: (i) P.M. Bauer et al. al.,“Role of p42 / p44 Mitogen-Activated-Protein Kinase and p21waf1 / cip1 in the Regulation of Vascular Smooth Muscle Cell Proliferation by Nitric Oxide,” Proc.Nat l.Acad.Sci.USA 98:12802-12807(2001);(ii) SHChoi et al., “Polyamine-Depletion Induces p27Kip1 and Enhances Dexamethasone-Induced G1 Arrest and Apoptosis in Human T lymphoblastic Leukemia Cells,” Leuk.Res.24:119-127(2000);(iii)H.Guo et al.,“RhoA Stimulates IEC-6 Cell Proliferation by Increasing Polyamine-Dependent Cdk2 Activity,”Am.J.Physiol.Gastrointest.Liver Physiol. 285:G704-713(2003);(iv)L.Li et al.,"JunD Stabilization Results in Inhibition of Normal Intestinal Epithelial Cell Growth through P21 after Polyamine Depletion,"Gastroenterology 123:764-779(2002);(V)M.Li et al.,“Chemoprevention of Mammary Carcinogenesis in a Transgenic Mouse Model by Alpha-Difluoromethylornithine(DFMO)in the Diet Is associated with Decreased Cyclin D1 Activity,” Oncogene 22:2568-2572(2003);(vi)A.Mohammed et al.,“Eflornithine(DFMO)Prevents Progression of Pancreatic Cancer by Modulating Ornithine Decarboxylase Signaling,” Cancer Prev.Res .7:1198-1209(2014) ;(vii)T.Nemoto et al.,"p53 Independent G(1)arrest Induced by DL-Alpha-Difluoromethylornithine," Biochem.Biophys.Res.Commun.280:848-854(2001);(viii)“R.M.Ray et al.,“Polyamine Depletion Arrests Cell Cycle and Induces Inhibitors p21(Waf1 / Cip1),p27(Kip1),and p53 in IEC-6 Cells,” Am. J Physiol .276:C684-691(1999);(ix)R.J.Rounbehler et al.,“Targeting Ornithine Decarboxylase Impairs Development of MYCN-Amplified Neuroblastoma,” Cancer Res.69:547-553(2009);(x)J.Singh et al.,“Modulation of Azoxymethane-Induced Mutational Activation of ras Protooncogenes by Chemopreventive Agents in Colon Carcinogenesis,” Carcinogenesis 15:1317-1323 (1994); (xi) R. Singh et al., “Activation of Caspase-3 Activity and Apoptosis in MDA-MB-468 Cells by N(omega)-Hydroxy-L-Arginine, an Inhibitor of Arginase, Is Not Solely Dependent on Reduction in Intracellular Polyamines,” Carcinogenesis 22:1863-1869(2001);(xii) L. Tao et al., “Altered Expression of c-myc,p16 and p27 in Rat Colon Tumors and Its Reversal by Short-Term Treatment with Chemopreventive Agents.”Carcinogenesis 23:1447-1454(2002);(x iii) CJWallick et al., “Key Role for p27Kip1,Retinoblastoma Protein Rb,and MYCN in Polyamine Inhibitor-Induced G1 Cell Cycle Arrest in MYCN-Amplified Human Neuroblastoma Cells,”Oncogene 24:5606-5618(2005);(xiv)Q. Xiang et al.,"[Apoptotic Induction of Human Lung Carcinoma A549 Cells by DFMO through Fas / FasL Pathway]," Ai Zheng 12:1260-1263 (2003); and references (9) and (10) below. .

[0196] The present invention will now be described with reference to the following examples, which are for illustrative purposes only and are not intended to limit the scope of the present invention. [Example]

[0197] Eflornithine toxicity Toxicity caused by eflornithine from the original phase 2 randomized trial (Levin et al., 1992) of AG and GBM patients treated at the time of recurrence is shown in Table 1. In this trial, eflornithine was administered at a dose of 3.6 g / m every 8 hours for 14 of 21 days. 2 At this dose, 13%, 16%, 3%, and 1% of patients receiving eflornithine reported diarrhea with toxicity grades of 1 to 4, respectively. It was noted that in exceptional cases, reducing the daily eflornithine dosage from three times daily to one divided dose four to six times daily proved effective in reducing diarrhea thought to be due to the osmotic load of oral eflornithine on the gastrointestinal tract. In this study, hematologic toxicity appeared to be very well tolerated, rarely requiring a single dose reduction of eflornithine.

[0198] [Table 1]

[0199] Table 2 summarizes the toxicity caused by eflornithine in combination with PCV in a Phase 3 trial (7) of eflornithine and PCV versus PCV for adjuvant, post-radiation chemotherapy in newly diagnosed AG and GBM patients (N=500). As used herein, the term "toxicity" refers to any one of the side effects listed in Table 2 above or any side effect that may occur during the course of treatment, regardless of the severity of the side effect. In this table, eflornithine at a dose of 3.0 g / m every 8 hours for 14 days out of 28 days was used. 2 We summarize specific toxicities that may be attributable to eflornithine in the eflornithine-PCV arm. The only adverse event that was statistically significantly more frequent in the eflornithine-PCV arm versus the PCV-alone arm was diarrhea (p=0.013).

[0200] [Table 2]

[0201] The following publications are incorporated herein by reference. These publications are identified herein by the numbers given below. Any publication in this publication list may be included. The inclusion of any reference herein should not be taken as an admission that any publication discussed herein is prior art. 1.Metcalf R, Bey P, Danzin C, Jung MJ, Casara P, Vevert JP.Catalytic irreversible inhibition of mammalian ornithine decarboxylase(EC 4.1.1.17) by substrate and analog product analogs.J Am Chem Soc.1978;100:2551-2552. 2.Bacchi CJ, Garofalo J, Mockenhaupt D, et al.In vivo effects of alpha-DL-difluoromethylornithine on the metabolism and morphology of Trypanosoma brucei brucei.Mol Biochem Parasitol.Mar 1983;7(3):209-225. 3.Bacchi CJ,Nathan HC,Hutner SH,McCann PP,Sjoerdsma A.Polyamine metabolism:a potential therapeutic target in trypanosomes.Science.Oct 17 1980;210(4467):332-334.4.Shantz LM,Levin VA.Regulation of ornithine decarboxylase during oncogenic transformation:mechanisms and therapeutic potential.Amino Acids.Aug 2007;33(2):213-223. 5.Childs AC,Mehta DJ,Gerner EW.Polyamine-dependent gene expression.Cell Mol Life Sci.Jul 2003;60(7):1394-1406. 6.Gerner EW,Meyskens FL,Jr.Polyamines and cancer:old molecules,new understanding.Nat Rev Cancer.Oct 2004;4(10):781-792. 7.Levin VA,Hess KR,Choucair A,et al.Phase III randomized study of postradiotherapy chemotherapy with combination alpha-difluoromethylornithine-PCV versus PCV for anaplastic gliomas.Clinical Cancer Research.Mar 2003;9(3):981-990. 8.Levin VA,Hess KR,Choucair AK,et al.Final report for evaluable patients treated on DM92-035,phase III randomized study of post-irradiation PCV versus DFMO-PCV,for anaplastic gliomas(AG).Neuro Oncol.2012;14(Supplement 6):vi74. 9.Koomoa DL,Yco LP,Borsics T,Wallick CJ,Bachmann AS.Ornithine decarboxylase inhibition by DFMO activates opposing signaling pathways via phosphorylation of both Akt / PKB and p27Kip1 in neuroblastoma.Cancer Res.Dec 1 2008;68(23):9825-9831. 10.Koomoa DL,Geerts D,Lange I,et al.DFMO / eflornithine inhibits migration and invasion downstream of MYCN and involves p2 7Kip1 activity in neuroblastoma.Int J Oncol.Apr 2013;42(4):1219-1228. 11.Johnson BE,Mazor T,Hong C,et al.Mutational Analysis Reveals the Origin and Therapy-Driven Evolution of Recurrent Glioma.Science.Dec 12 2013. 12.Hunter C,Smith R,Cahill DP,et al.A hypermutation phenotype and somatic MSH6 mutations in recurrent human malignant gliomas after alkylator chemotherapy.Cancer Res.Apr 15 2006;66(8):3987-3991. 13.Yip S,Miao J,Cahill DP,et al.MSH6 mutations arise in glioblastomas during temozolomide therapy and mediate temozolomide resistance.Clin Cancer Res.Jul 15 2009;15(14):4622-4629. 14.The Cancer Genome Atlas Research Network.Comprehensive genomic characterization defines human glioblastoma genes and core pathways.Nature.2008;455:1061-1068.15.Bodell WJ,Gaikwad NW,Miller D,Berger MS.Formation of DNA adducts and induction of lacI mutations in Big Blue Rat-2 cells treated with temozolomide:implications for the treatment of low-grade adult and pediatric brain tumors.Cancer Epidemiol Biomarkers Prev.Jun 2003;12(6):545-551. 16.Einspahr JG,Nelson MA,Saboda K,Warneke J,Bowden GT,Alberts DS.Modulation of biologic endpoints by topical difluoromethylornithine(DFMO),in subjects at high-risk for nonmelanoma skin cancer.Clin Cancer Res.Jan 2002;8(1):149-155. 17.Hoshino T,Prados M,Wilson CB,Cho KG,Lee KS,Davis RL.Prognostic implications of the bromodeoxyuridine labeling index of human gliomas.J Neurosurg.1989;71(3):335-341. 18.Labrousse F,Daumas-Duport C,Batorski L,Hoshino T.Histological grading and bromodeoxyuridine labeling index of astrocytomas.Comparative study in a series of 60 cases.J Neurosurg.1991;75(2):202-205. 19.Prados MD,Krouwer HG,Edwards MS,Cogen PH,Davis RL,Hoshino T.PROLIFERATIVE POTENTIAL AND OUTCOME IN PEDIATRIC ASTROCYTIC TUMORS.J Neurooncol.1992;13(3):277-282. 20.Hoshino T,Ahn D,Prados MD,Lamborn K,W ilson CB.Prognostic significance of the proliferative potential of intracranial gliomas measured by bromodeoxyuridine labeling.Int J Cancer.1993 1993;53(4):550-555. 21.Ito S,Chandler KL,Prados MD,et al.Proliferative potential and prognostic evaluation of low-grade astrocytomas.J Neuro-Oncol.1994 1994;19(1):1-9. 22.Onda K,Davis RL,Shibuya M,Wilson CB,Hoshino T.Correlation between the bromodeoxyuridine labeling index and the MIB-1 and Ki-67 proliferating cell indices in cerebral gliomas.Cancer.1994 1994;74(7):1921-1926. 23. Kajiwara Y, Panchabhai S, Levin VA. A new preclinical 3-dimensional agarose colony formation assay. Technol Cancer Res Treat. Aug 2008;7(4):329-334. 24. Kajiwara Y, Panchabhai S, Liu DD, Kong M, Lee JJ, Levin VA. Melding a New 3-Dimensional Agarose Colony Assay with the E(max) Model to Determine the Effects of Drug Combinations on Cancer Cells. Technol Cancer Res Treat. Apr 2009;8(2):163-176. 25. Levin VA, Panchabhai SC, Shen L, Kornblau SM, Qiu Y, Baggerly KA. Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines. J Proteome Res. Jan 2010;9(1):179-191. 26. Levin VA, Panchabhai S, Shen L, Baggerly KA. Protein and phosphoprotein levels in glioma and adenocarcinoma cell lines grown in normoxia and hypoxia in monolayer and three-dimensional cultures. Proteome Sci. Jan 25 2012;10(1):5. [Effects of the Invention] The present invention, including the methods and compositions described herein, provides novel and effective methods for treating gliomas, particularly low-grade (WHO grade II) and intermediate-grade (WHO grade III) gliomas, and can prevent these gliomas from progressing to higher grades. The methods and compositions of the present invention can prevent anaplastic gliomas, particularly anaplastic astrocytomas, from progressing to more aggressive phenotypes, such as glioblastomas. The methods and compositions are well tolerated, do not produce significant side effects, and can be used in conjunction with other anti-neoplastic agents.

[0202] The method according to the present invention has industrial applicability for preparing a medicament for treating glioma. The composition according to the present invention has industrial applicability, particularly as a pharmaceutical composition for treating glioma.

[0203] The method claims of the present invention provide specific method steps that go beyond the general application of laws of nature, requiring one practicing the method steps to employ steps other than those conventionally known in the art in addition to the specific application of laws of nature described or encompassed in the claims, thereby limiting the scope of the claims to the specific applications described in the claims. In some circumstances, these claims may also be directed to new methods of using existing drugs.

[0204] The invention illustratively described herein may suitably be practiced in the absence of any element or limitation not specifically disclosed herein. For example, the terms "comprise," "include," "contain," and the like are to be interpreted broadly and without limitation. Furthermore, the terms and expressions used herein are used as descriptive, not limiting, terms that exclude any future equivalents shown and described, or any portion thereof, and it is understood that various modifications are possible within the scope of the invention as claimed. That is, while the invention has been specifically disclosed with preferred embodiments and optional features, it should be understood that modifications and variations of the invention disclosed herein may be rediscovered by those skilled in the art, and that such modifications and variations are also deemed to be within the scope of the invention disclosed herein. The inventions are described broadly and generically herein. Narrower species and subgeneric groupings falling within the scope of the generic disclosure also form part of these inventions. In this case, the generic description of each invention is included with any provisos or negative limitations excluding all subject matter from the genus, regardless of whether the omitted material was expressly present therein.

[0205] Furthermore, where features or aspects of the invention are described in terms of a Markush group, those trained in the art will recognize that the invention is also thereby described in terms of any individual member or subgroup of members of the Markush group. It is also understood that the above description is illustrative and not intended to be limiting. Many embodiments will become apparent to those skilled in the art upon review of the above description. The scope of the invention should, therefore, be determined not with reference to the above description, but with reference to the appended claims, along with the full scope of equivalents to which such claims are entitled. The disclosures of all articles and references, including patent publications, are incorporated herein by reference.

Claims

1. 1. A pharmaceutical composition for treating glioma in a patient previously treated with temozolomide, comprising: (a) a therapeutically effective amount of eflornithine or a pharmaceutically acceptable salt of eflornithine; (b) a pharmaceutically acceptable carrier; The pharmaceutical composition, wherein the glioma has a mutation in one or more genes selected from the group consisting of IDH1, IDH2, TP53, PTEN, and ATRX.

2. The pharmaceutical composition according to claim 1, characterized in that the pharmaceutical composition slows the progression of the glioma.

3. The pharmaceutical composition of claim 1 , wherein the glioma is an anaplastic astrocytoma.

4. 2. The pharmaceutical composition of claim 1, wherein the eflornithine or pharmaceutically acceptable salt of eflornithine is eflornithine.

5. 5. The pharmaceutical composition of claim 4, wherein the eflornithine or pharmaceutically acceptable eflornithine salt is an eflornithine salt.

6. 5. The pharmaceutical composition of claim 4, wherein the eflornithine is a racemic mixture of D-eflornithine and L-eflornithine.

7. 5. The pharmaceutical composition of claim 4, wherein the eflornithine is D-eflornithine.

8. 5. The pharmaceutical composition of claim 4, wherein the eflornithine is L-eflornithine.

9. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is administered orally.

10. The pharmaceutical composition according to claim 1 , wherein the glioma has a mutation in the IDH1 gene.

11. The pharmaceutical composition according to claim 1, wherein the glioma has a mutation in the RB pathway and the AKT-mTOR pathway.

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