Closed-type cell separation device

Abstract

A closed cell separation and manipulation device is provided. [Solution] The closed type cell separation and manipulation device has a first tube (10) having an air hole and an inlet, a second tube (20) having an air hole (131) and an inlet, and a connecting tube (24) connecting the first tube (10) and the second tube (20), wherein an air hole (131) is provided on the first tube (10) to allow air to flow in and out of the first tube (10), the first tube (10) has an inlet (132) to allow fluid from an external fluid source to enter the first tube (10), the first tube (10) has an outlet (121), and wherein an air hole (231) is provided on the second tube (20) to allow air to flow in and out of the second tube (20), the second tube (20) allows a portion of the fluid from the outlet (121) of the first tube (10) to enter the inlet (232) of the second tube (20), and the second tube (20) has an outlet (221), and the closed type cell separation and manipulation device can provide a closed and contamination-free environment during centrifugation and during supernatant liquid separation after centrifugation.

Description

[Technical Field]

[0001] The present invention relates to the technical field of cell centrifugation, and more particularly to a closed cell separation and operation device that can provide a closed, contamination-free operating environment for separating supernatant liquid during and after centrifugation. [Background technology]

[0002] Centrifugation is a technique commonly used in cell-related research and testing to separate cells from specimens such as concentrated bone marrow aspirate (BMAC), cerebrospinal fluid (CSF), peripheral blood, urine, ascites, and cell culture media. Centrifuge tubes are essential tools for centrifugation, and are commonly test tubes with lids, such as those sold under the name Nunc (Thermo Fisher Scientific, USA). Summary of the Invention [Problem to be solved by the invention]

[0003] According to the prior art, the fluid to be centrifuged must be poured into a centrifuge tube, which must then be sealed and placed in a centrifuge for processing. After centrifugation, to remove the separated supernatant (such as plasma when centrifuging whole blood) and obtain the sediment (such as blood cells when centrifuging whole blood), or vice versa, the operator must open the test tube and pour the supernatant into a secondary container, allowing the two parts to be separated. However, this procedure necessarily exposes the supernatant and sediment to the surrounding environment, creating a risk of contamination. One solution to avoid this concern is to perform the entire procedure in a clean room or biological safety cabinet (BSC), but the associated equipment is expensive, difficult to transport, and not suitable for clinical use.

[0004] Furthermore, the conventional method of separating the supernatant after centrifugation has the problem that droplets are generated when the contents of the centrifuge tube are transferred, reducing the amount of sample that can be used. This could have a negative impact on subsequent research or testing, or even prevent research or testing from being carried out at all. Therefore, there is a need in the art for a new post-centrifugation supernatant separation device that can effectively separate cells while preventing sample contamination, avoiding the risk of sample splashing, and eliminating the need to purchase expensive laboratory equipment. [Means for solving the problem]

[0005] Based on the above needs, the main object of the present invention is to provide a closed cell separation and manipulation device that can provide a sealed and contamination-free environment for separating the supernatant during or after centrifugation while avoiding the risk of sample scattering, and does not require the purchase of expensive laboratory equipment.

[0006] The closed cell separation and manipulation device according to the present invention has a first tubular body, a second tubular body, and a connecting tube, The first pipe is a cylindrical container having a peripheral wall, the first pipe has a closed top and is in closable communication with the outside through a pore and an inlet, the pore being a pore that allows gas to pass through the first pipe to enter and exit the first pipe, the inlet being an inlet that allows fluid from an external fluid source to enter the first pipe, the first pipe has a closed bottom and is in closable communication with the outside through a central outlet, the second pipe is a cylindrical container having a peripheral wall, the second pipe has a closed top and communicates with the outside in a closable manner through a pore and an inlet, the pore being a pore that allows gas to pass through and enter and exit the second pipe, the inlet allowing at least a portion of the fluid from the outlet of the first pipe to enter the inlet of the second pipe, the second pipe has a closed bottom and communicates with the outside in a closable manner through an outlet formed in the center; The connecting pipe is disposed between the outlet of the first pipe and the inlet of the second pipe, connecting the first pipe and the second pipe.

[0007] Furthermore, the peripheral walls of the first and second pipe bodies extend downward toward the bottom of the pipe bodies, and the peripheral walls and the bottom form a pipe storage space around the outlet of the pipe body, and at least a portion of the connecting pipe and / or at least a portion of the extension pipe are wrapped around the outlet and stored in the pipe storage space.

[0008] Furthermore, the closed-type cell separation and manipulation device further has an extension tube installed at the outlet of the second tube, and the second tube is connected to another first tube of another closed-type cell separation and manipulation device through the extension tube.

[0009] Furthermore, a tank opening is formed on at least one lower edge of the peripheral wall of the first pipe body and the second pipe body to allow the connecting pipe to pass through.Therefore, when the first pipe body and the second pipe body are stood on a flat surface, a passage is left in the pipe storage space of the first pipe body / second pipe body, through which the connecting pipe can pass.

[0010] Furthermore, at least one of the connecting pipe and the extension pipe is provided with a valve component for closing or opening the connecting pipe or the extension pipe, and the valve component is selected from at least one of a pipe wrench, a flow control valve, a three-way faucet connector, and a fluid switching valve.

[0011] Furthermore, the bottom of at least one of the first and second tubes has a downwardly converging conical structure, the diameter of which gradually decreases and reaches a minimum at the outlet of the first and / or second tube.

[0012] Furthermore, at least one of the pores of the first and second tubular bodies is provided with a filter program for filtering air passing through the pore.

[0013] Furthermore, the closed cell separation and manipulation device further includes an air pressure source disposed in at least one air hole in the first and second tubular bodies, the air pressure source being used to push or pull air into or out of the first tubular body and / or the second tubular body, and the air pressure source being an air pump or an air injector.

[0014] Furthermore, at least one of the inlets and the holes of the first and second pipes is provided with a sealing member that is sealed in an airtight manner.

[0015] Furthermore, at least one of the inlets and pores of the first and second pipes is provided with a conduit for accommodating a valve component.

[0016] The valve component is selected from at least one of a pipe wrench, a flow control valve, a three-way faucet connector, and a fluid switching valve.

[0017] The effects of the present invention are as follows. The closed cell separation and manipulation device of the present invention can provide a sealed, contamination-free environment for separating the supernatant during or after centrifugation while avoiding the risk of specimen scattering, and does not require the purchase of expensive laboratory equipment. [Brief explanation of the drawings]

[0018] [Figure 1] 1 is a perspective view of a closed cell separation and manipulation device according to an embodiment of the present invention. [Figure 2] 1 is a cross-sectional view of an application of a closed cell separation and manipulation device according to the present invention, showing an operating state during centrifugation using the closed cell separation and manipulation device. [Figure 3] 1 is a cross-sectional view of an application of a closed cell separation and manipulation device according to the present invention, showing the operation state of the supernatant separation during and after centrifugation using the closed cell separation and manipulation device. [Figure 4] 1 is a cross-sectional view of an application of the closed cell separation and manipulation device according to the present invention, showing the operation state of supernatant separation after centrifugation using the closed cell separation and manipulation device. [Figure 5] FIG. 10 is a perspective view of another embodiment in which two closed cell separation and manipulation devices according to the present invention are connected in series. [Figure 6] FIG. 10 is a perspective view of a closed cell separation and manipulation device according to another embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0019] (One embodiment) The concepts of the present invention are described below with reference to preferred embodiments, however, parts of the embodiments shown in the figures are for convenience of illustration only and are not necessarily drawn to scale. In this specification and claims, the singular form "a" or "an" includes plural references unless the context dictates otherwise.

[0020] As shown in Figures 1 and 2, according to one embodiment of the present invention, a closed cell separation and manipulation device has a first tubular body 10 having a first cap 13, a second tubular body 20 having a second cap 23, and a connecting tube 24 connecting the first tubular body 10 and the second tubular body 20.

[0021] The first tube 10 and the second tube 20 are structurally identical, and for the sake of brevity, only one of them will be described below. The first pipe 10 or the second pipe 20 is a cylindrical container surrounded by a peripheral wall 122 or 222 . The first pipe 10 or the second pipe 20 has a top that is completely open to the outside through an opening 11 or 21, and a bottom 12 or 22 that is closed but forms an external outlet 121 or 221 in the center. Specifically, the bottom 12 or 22 has a funnel-like shape and a tapered structure, with the diameter gradually decreasing from top to bottom, reaching a minimum around the outlet 121 or 221 . The peripheral wall 122 or 222 of the first pipe 10 or the second pipe 20 extends downward beyond the closed bottom 12 or 22, so that together with the funnel-shaped bottom 12 or 22, they jointly form a pipe storage space 123 or 223 around the outlet 121 or 221. The lower edge of the peripheral wall 122 or 222 is further formed with a tank opening 124 or 224, so that when the pipe body 10 or 20 is placed upright on a flat surface, the pipe receiving space 123 or 223 forms a passage. The size of the first tubular body 10 or the second tubular body 20 is equivalent to that of a conventional centrifuge tube or blood collection tube, and therefore can be used in a conventional centrifuge.

[0022] Similarly, the first cap 13 and the second cap 23 are structurally identical, and for the sake of brevity, only one of them will be described below. The first cap 13 or the second cap 23 can cover the top of the first pipe 10 or the second pipe 20 from top to bottom, and has an air hole 131 or 231 and an inlet 132 or 232. As will be explained in more detail below, the vent 131 or 231 and the inlet 132 or 232 are each configured to be removably connected to a conduit during use, or removably sealed with a sealing member 80 when not in use.

[0023] In this embodiment, the first cap 13 or the second cap 23 on the first pipe 10 or the second pipe 20 has a hole 131 or 231 and an inlet 132 or 232 . However, in other embodiments, the vent 131 or 231 and inlet 132 or 232 of the closed cell separation and manipulation device of the present invention can be installed directly on the closed top of the first tube 10 or the second tube 20, without using a cap 13 or 23 to cover the first tube 10 or the second tube 20.

[0024] The connecting pipe 24 is disposed between the outlet 121 of the first tube 10 and the inlet 232 of the second tube 20, thereby creating fluid communication between the first tube 10 and the second tube 20. A portion of the connecting pipe 24 is wound around the outlet 121 of the first pipe body 10 and is appropriately housed in the pipe housing space 123 of the first pipe body 10 . A tank opening 124 is formed on the peripheral wall 122 of the first pipe 10, so that the connecting pipe 24 extends between the first pipe 10 and the second pipe 20, and does not affect the stability of the first pipe 10 standing on a flat surface. The connecting pipe 24 is further provided with a valve element 60 for opening or closing the connecting pipe 24 .

[0025] In use, when the connecting tube 24 is closed by the valve component 60, the inlet 132 of the first tubular body 10 is connected to the fluid supply tube 40, which communicates with a source of fluid to be processed (such as a blood bag, blood collection needle, or specimen tube, not shown). At this time, the pores 131 of the first tubular body 10 remain open, allowing the fluid to be processed (such as whole blood) to enter the first tubular body 10 (see FIG. 2). Here, a filter program can be installed in the pores 131 of the first tubular body 10 to prevent foreign matter from entering the first tubular body 10 and ensure a contamination-free operating environment. Specifically, as shown in FIG. 2, the air permeable pipe 30 in which the filter program 31 is installed and the pores 131 of the first pipe body 10 are connected to each other. The air tube 30 is similarly controlled by a valve component 60 to be opened or closed. After the first pipe 10 is filled with fluid, the sealing member 80 is used to airtightly cover the inlet 132 of the first pipe 10, or the sealing member 80 is used to airtightly cover the liquid supply pipe 40 connected to the inlet 132 of the first pipe 10 (see FIG. 1). Similarly, the sealing member 80 can be used to directly and airtightly cover the air hole 131 of the first tubular body 10, or the sealing member 80 can be used to airtightly cover the air permeable pipe 30 connected to the position of the air hole 131 (see Figure 1).

[0026] It should be noted here that when the connecting pipe 24, the air permeation pipe 30, and the liquid supply pipe 40 are closed by the valve parts 60, the inside and outside of the first pipe body 10 are separated to form a closed environment. Similarly, at this time, the pores 231 and the outlet 221 of the second tubular body 20 are also closed, thus isolating it from the outside.

[0027] Next, the entire closed cell separation and manipulation device is moved into a centrifuge (not shown) for centrifugation. During centrifugation, the first tube 10 and the second tube 20 are positioned at two adjacent tube locations within the centrifuge. At this time, a part of the connecting pipe 24 is wrapped around the outlet 121 and is housed in the pipe housing space 123 of the first pipe body 10 . In this way, excessive vibration of the connecting pipe 24 during the centrifugal operation can be prevented.

[0028] As shown in FIG. 3, at this point, the fluid in the first tubular body 10 has already been separated into a supernatant (such as plasma when whole blood is centrifuged) and a precipitate (such as blood cells when whole blood is centrifuged). Next, when transferring the sediment to the second pipe 20, first open the valve assembly 60 on the connecting pipe 24, and remove the caps 80 on the air permeable pipe 30 of the first pipe 10 and the air hole 231 of the second pipe 20, respectively. The air pressure source 70 can be selectively and removably connected to the air hole 231 of the second tube 20, thereby utilizing suction force to accelerate the rate of sediment migration.

[0029] The bottom 12 of the first tube 10 is funnel-shaped, i.e., has a downwardly conical structure, and the outlet 121 is at the lowest position of the bottom 12 of the first tube 10, so the operator can precisely control the movement of the sediment and there is no risk of the supernatant liquid inadvertently entering the second tube 20. Furthermore, since the sediment flows directly into the second pipe 20, the risk of scattering can be completely avoided. Once the sediment has entered the second tube 20, the valve assembly 60 is used to close the connecting tube 24 and re-cap the vent 231 of the second tube 20 after the pressure source 70 has been removed. In this way, the supernatant liquid and the sediment are sealed in the first tubular body 10 and the second tubular body 20, respectively. Through the above structure, highly efficient and reliable separation of the precipitate and supernatant can be achieved (see Figure 4).

[0030] As shown in FIG. 5, in various applications requiring multiple separations, another closed cell separation and manipulation device according to the present invention is connected in series after the first closed cell separation and manipulation device. Specifically, an extension tube 50 is used and connected to the first tube 10 of the second closed-type cell separation and manipulation device. In this situation, the elongated tube 50 can be wrapped around the outlet 221 and housed in the pipe housing space 223 of the second tube body 20 before centrifugation, similar to the connecting tube 24 described above.

[0031] FIG. 6 shows another embodiment of the closed cell separation and manipulation device of the present invention. As shown in FIG. 6, the closed-type cell separation and manipulation device according to the present invention can further be provided with a second air permeable tube 90 attached to the air hole 231 of the second tubular body 20 . Similarly, the second air permeable pipe 90 is provided with a valve component 60 for opening and closing the same. A separate filter program 91 is connected to the second air permeable pipe 90 to prevent foreign matter from entering the second pipe body 20 and to ensure a contamination-free operating environment. In this situation, the air pressure source 70 described above can be connected to the vent 131 of the first tube 10 to force the contents of the first tube 10 into the second tube 20 .

[0032] The above-mentioned valve components 60 may be, but are not limited to, a pipe wrench, a flow control valve, a three-way faucet connector, or a fluid switching valve. The air pressure source 70 attached to the air hole 131 of the first tube 10 and / or the air hole 231 of the second tube 20 may be an air pump or an air injector, or any other device that can change the pressure in the first tube 10, the second tube 20, and promote the flow of fluid from the first tube 10 to the second tube 20.

[0033] Centrifugation is widely used in a variety of medical and biological applications and is well known to those skilled in the art. Therefore, for the sake of brevity, the operations and principles involved will not be described in detail. Similarly, the cell separation technique using the closed cell separation and manipulation device according to the present invention is well known to technical personnel in this field, and therefore a detailed description thereof will be omitted here.

[0034] With the above configuration, the closed cell separation and manipulation device of the present invention can avoid the risk of scattering, eliminate the need to purchase expensive laboratory equipment, and provide a closed, contamination-free environment for the supernatant separation operation during and after centrifugation.

[0035] The above are only preferred embodiments of the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall fall within the scope of protection of the present invention. [Explanation of symbols]

[0036] 10 first pipe body, 11, 21 aperture, 121, 221 exit, 12, 22 bottom, 122, 222 peripheral wall, 123, 223 pipe storage space, 124, 224 Tankguchi, 13 First cap, 131, 231 stomata, 132, 232 entrance, 20 second pipe, 23 Second cap, 24 connecting pipe, 30 permeable pipe, 31 filter programs, 40 liquid advance tube, 50 stretched tube, 60 valve parts, 70 barometric pressure source, 80 sealing member, 90 Second air permeable pipe, 91 Extra-frame filter program.

Claims

1. A closed cell separation and manipulation device having a first tubular body, a second tubular body, and a connecting tube, The first pipe is a cylindrical container having a peripheral wall, the first pipe has a closed top and is in communication with the outside in a closable manner through a pore and an inlet, the pore is a pore that allows gas to pass through and enter and exit the first pipe, the inlet is an inlet that allows fluid from an external fluid source to enter the first pipe, the first pipe has a closed bottom and is in communication with the outside in a closable manner through an outlet formed in the center, The second pipe is a cylindrical container having a peripheral wall, the second pipe has a closed top and communicates with the outside in a closable manner through a pore and an inlet, the pore is a pore that allows gas to pass through and enter and exit the second pipe, the inlet allows at least a portion of fluid from the outlet of the first pipe to enter the inlet of the second pipe, the second pipe has a closed bottom and communicates with the outside in a closable manner through an outlet formed in the center, The connecting pipe is installed between the outlet of the first pipe and the inlet of the second pipe, and the first pipe is connected to the second pipe through the connecting pipe; The peripheral wall of the first pipe body extends downward toward a bottom portion of the first pipe body, and the peripheral wall and the bottom portion form a pipe storage space around the outlet of the first pipe body. Closed cell separation operation device.

2. A tank opening through which the connecting pipe passes is formed at the lower edge of the peripheral wall of the first pipe body. The closed cell separation and manipulation device according to claim 1 .

3. A closed-type cell separation and manipulation device, comprising a first tubular body, a second tubular body, and a connecting tube, The first pipe is a cylindrical container having a peripheral wall, the first pipe has a closed top and is in communication with the outside in a closable manner through a pore and an inlet, the pore is a pore that allows gas to pass through and enter and exit the first pipe, the inlet is an inlet that allows fluid from an external fluid source to enter the first pipe, the first pipe has a closed bottom and is in communication with the outside in a closable manner through an outlet formed in the center, The second pipe is a cylindrical container having a peripheral wall, the second pipe has a closed top and communicates with the outside in a closable manner through a pore and an inlet, the pore is a pore that allows gas to pass through and enter and exit the second pipe, the inlet allows at least a portion of fluid from the outlet of the first pipe to enter the inlet of the second pipe, the second pipe has a closed bottom and communicates with the outside in a closable manner through an outlet formed in the center, The connecting pipe is installed between the outlet of the first pipe and the inlet of the second pipe, and the first pipe is connected to the second pipe through the connecting pipe; The closed cell separation and manipulation device further has an extension tube installed at the outlet of the second tube, and the second tube is connected to another first tube of another closed cell separation and manipulation device through the extension tube, The peripheral wall of the second pipe extends downward toward the bottom of the second pipe, and the peripheral wall and the bottom form a pipe storage space around the outlet of the second pipe. Closed cell separation operation device.

4. A tank opening through which the connecting pipe passes is formed at the lower edge of the peripheral wall of the second pipe body. The closed cell separation and manipulation device according to claim 3 .

5. The connecting pipe is provided with a valve part for closing or opening the connecting pipe. The closed cell separation and manipulation device according to claim 1 or 3.

6. The extension pipe is provided with a valve part for closing or opening the connecting pipe. The closed cell separation and manipulation device according to claim 3 .

7. the bottom of the first pipe has a conical structure that converges downward, and the diameter of the conical structure gradually decreases and becomes smallest at the outlet of the first pipe; The closed cell separation and manipulation device according to claim 1 .

8. the bottom of the second pipe has a conical structure that converges downward, and the diameter of the conical structure gradually decreases and becomes smallest at the outlet of the second pipe; The closed cell separation and manipulation device according to claim 3 .

9. At least one of the pores of the first tube and the second tube has a filter program for filtering air passing through the pore. The closed cell separation and manipulation device according to claim 1 or 3.

10. At least one of the air holes in the first tube and the second tube has a pressure source for forcing air into or venting air from the first tube and / or the second tube. The closed cell separation and manipulation device according to claim 1 or 3.

11. At least one of the inlets and the air holes of the first and second pipes is provided with a sealing member that is airtightly sealed. The closed cell separation and manipulation device according to claim 1 or 3.

12. At least one of the inlet and the pore of the first and second pipes is further provided with a conduit for accommodating a valve component. The closed cell separation and manipulation device according to claim 1 or 3.

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