Table type glass bioreactor

Abstract

The utility model discloses a table type glass bioreactor and relates to the technical field of bioreactors. The bioreactor comprises a bioreactor body, wherein a sealing cover is arranged at the top of the bioreactor body. The moving mechanism can drive the stirring mechanism to rotate while moving up and down, so that the stirring mechanism is in a working state of rotating while moving up and down in the bioreactor body, and high-temperature liquid positioned below the bioreactor body can flow to an upper area; meanwhile, the liquid with lower temperature above the bioreactor body flows to the area with higher temperature below the bioreactor body, so that the temperature gradient of different height positions in the bioreactor body is broken, even in the area far away from the heating plate, the uniform distribution of the temperature can be realized, and the consistency of the temperature in the bioreactor is improved; and biological processes such as cell culture and fermentation are facilitated.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of bioreactor, concretely, especially relates to a table type glass bioreactor. BACKGROUND

[0002] As the core equipment for cell culture, fermentation and other key processes in the field of biotechnology, the performance of the bioreactor directly affects the quality and yield of biological products. Among the many types of bioreactors, the table type glass bioreactor is widely used in laboratory research and small-scale production due to its good visibility, easy operation and cleaning, and other advantages.

[0003] The common heating method is to set a heating plate at the bottom of the reactor. This single heat source layout makes the heat transfer upward. Due to the thermal conductivity characteristics of the glass material and the difference in the convection of the liquid in the reactor, there is a clear temperature gradient at different heights in the reactor. The temperature is high near the bottom of the heating plate, while the temperature is relatively low in the upper area far from the bottom. Although stirring can promote the mixing of the liquid in the reactor and help the uniform distribution of temperature, the existing stirring can only help the diffusion of the liquid temperature with the same gradient, and there is still a significant temperature difference in the place far from the heating plate.

[0004] In view of the problems in the related art, no effective solution has been proposed so far. UTILITY MODEL CONTENT

[0005] In view of the problems in the related art, the utility model proposes a table type glass bioreactor to overcome the above technical problems existing in the prior art.

[0006] To solve the above technical problems, the utility model is realized by the following technical scheme:

[0007] The utility model discloses a table type glass bioreactor, including the bioreactor body, the top of bioreactor body is equipped with the sealing cover, the top fixed mounting of sealing cover has the support cylinder, the top of sealing cover is equipped with temperature probe, and the probe of temperature probe reaches the inside cavity of bioreactor body, and its characterized in that: the top of support cylinder is equipped with drive mechanism, and the drive mechanism is installed on the symmetry of guide strip, and the inside cavity fixed mounting of support cylinder has the ring guide block, and the output of two guide strips and drive mechanism installs the moving mechanism, and the top of moving mechanism is close to ring guide block bottom, and the bottom of ring guide block is close to the end of moving mechanism and is connected in the wave shape of head and tail, and the bottom of moving mechanism and the inside cavity bottom of support cylinder are equipped with the fit elastic component, and the fit elastic component is slidably installed with support cylinder, and the bottom of moving mechanism and located the inside cavity of bioreactor body install stirring mechanism, and the bottom of bioreactor body installs the heating plate.

[0008] Further, the drive mechanism includes a drive motor, the drive motor is fixedly installed on the top of the support cylinder, the output end of the drive motor is fixedly installed with the output shaft, and two guide strips are fixedly installed on the outer wall of the output shaft.

[0009] Further, the moving mechanism includes a connecting block, the connecting block is sleeved on the output shaft and two guide strips, and the bottom of the connecting block is fixedly installed with a moving rod.

[0010] Further, the top of the connecting block is fixedly installed with a connecting rod, the top of the connecting rod is provided with a ball, and the ball is close to the wave shape of the ring guide block.

[0011] Further, the fit elastic component includes a fixed block, the fixed block is fixedly installed on the inner wall bottom of the support cylinder, the top of the fixed block is fixedly installed with a spring, and the other end of the spring is fixedly installed with a round block.

[0012] Further, a plurality of sliding blocks are fixedly installed on the outer wall of the round block, a plurality of sliding blocks are slidably installed on the support cylinder, and the round block is located at the bottom of the connecting block, and the spring is sleeved on the moving rod.

[0013] Further, the stirring mechanism includes a stirring shaft, the stirring shaft is fixedly installed at the bottom of the moving rod, and a plurality of stirring discs are installed on the circumferential surface of the stirring shaft.

[0014] Further, a plurality of stirring plates are circumferentially arranged on the outer wall of the plurality of stirring discs, and the plurality of stirring plates are all arranged in an inclined manner.

[0015] Further, the bottom of the heating plate is provided with a base.

[0016] The utility model has the advantages of the following:

[0017] The utility model discloses a stirring mechanism can be rotated and moved along with the moving mechanism, and the stirring mechanism can stir the liquid in the bioreactor body, and the moving mechanism can drive the stirring mechanism to rotate while moving up and down, which makes the stirring mechanism present a working state of rotating and moving up and down in the bioreactor body, and the liquid with a higher temperature below the bioreactor body can flow to the upper area, and the liquid with a lower temperature above the bioreactor body can flow to the area below with a higher temperature, so that the temperature gradient at different heights in the bioreactor body is broken, and the temperature can be uniformly distributed even in the area far away from the heating plate, thereby improving the consistency of the temperature in the bioreactor and being beneficial to the biological processes such as cell culture and fermentation.

[0018] Of course, any product implementing the utility model does not necessarily need to achieve all the advantages mentioned above. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical scheme of the embodiments of the utility model, the following will briefly introduce the drawings needed to be used in the embodiment description, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creative labor.

[0020] Figure 1 It is the three-dimensional structure diagram of the utility model;

[0021] Figure 2 It is the bioreactor body sectional view and internal schematic view of the utility model;

[0022] Figure 3 It is the sectional view of the support cylinder and the whole schematic diagram of the stirring mechanism of the utility model;

[0023] Figure 4 It is the Figure 3 Enlarged view of A in the middle;

[0024] Figure 5 It is the whole schematic diagram of the moving mechanism of the utility model;

[0025] Figure 6 It is the whole schematic diagram of the elastic assembly of the utility model.

[0026] In the drawings, the component list represented by each sign is as follows:

[0027] 1, bioreactor body; 2, sealing cover; 3, support cylinder; 4, temperature probe; 5, driving mechanism; 501, driving motor; 502, output shaft; 6, guide bar; 7, circular ring guide block; 8, moving mechanism; 801, connecting block; 802, moving rod; 803, connecting rod; 804, ball; 9, fit elastic assembly; 901, fixed block; 902, spring; 903, round block; 904, sliding block; 10, stirring mechanism; 1001, stirring shaft; 1002, stirring disc; 1003, stirring plate; 11, heating plate; 12, base. DETAILED DESCRIPTION

[0028] The technical solutions in the utility model embodiments will be clearly and completely described below with reference to the drawings in the utility model embodiments. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0029] In the description of the utility model, it should be understood that the terms "opening", "upper", "lower", "top", "middle", "inner" and the like indicate the orientation or positional relationship, which are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the indicated components or elements must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the utility model.

[0030] Please refer to Figures 1-6 As shown in the drawing, the utility model is a table type glass bioreactor, which comprises a bioreactor body 1, the top of the bioreactor body 1 is provided with a sealing cover 2, the top of the sealing cover 2 is fixedly installed with a support cylinder 3, the top of the sealing cover 2 is provided with a temperature probe 4, the probe of the temperature probe 4 extends into the inner cavity of the bioreactor body 1, the top of the support cylinder 3 is provided with a driving mechanism 5, the driving mechanism 5 is symmetrically installed with a guide bar 6, the inner cavity of the support cylinder 3 is fixedly installed with a circular ring guide block 7, a moving mechanism 8 is installed on the output end of the two guide bars 6 and the driving mechanism 5, the top end of the moving mechanism 8 is close to the bottom end of the circular ring guide block 7, and the bottom of the circular ring guide block 7 is arranged in a wave shape close to the end of the moving mechanism 8, a fit elastic assembly 9 is arranged between the bottom of the moving mechanism 8 and the bottom of the inner cavity of the support cylinder 3, the fit elastic assembly 9 is slidingly installed with the support cylinder 3, a stirring mechanism 10 is installed at the bottom of the moving mechanism 8 and in the inner cavity of the bioreactor body 1, and a heating plate 11 is installed at the bottom of the bioreactor body 1.

[0031] In the bioreactor, first, the heating plate 11 is installed at the bottom of the bioreactor body 1, which provides heat source for the whole bioreactor, the probe of the temperature probe 4 extends into the cavity of the bioreactor body 1 to monitor the temperature in the bioreactor body 1 in real time, and the temperature data is fed back to the control system, so that the control system adjusts the heating power and other parameters of the heating plate 11 to maintain the appropriate temperature required for the reaction. It is important to note that the heating plate 11, temperature probe 4 and control system are prior art and will not be described in detail here. The control system is not shown in the figure.

[0032] When the liquid in the bioreactor body 1 needs to be stirred, the driving mechanism 5 is operated, the driving mechanism 5 is installed at the top of the support cylinder 3, the symmetrically arranged guide strips 6 are connected with the moving mechanism 8, and the output end of the driving mechanism 5 is also connected with the moving mechanism 8. During the operation of the driving mechanism 5, the moving mechanism 8 and the guide strips 6 will rotate in the support cylinder 3, the top end of the moving mechanism 8 is tightly attached to the bottom end of the circular ring guide block 7, and the bottom of the circular ring guide block 7 is designed in a wave shape. When the moving mechanism 8 rotates under the driving of the driving mechanism 5, due to the wave shape of the circular ring guide block 7, the moving mechanism 8 will also move up and down along the wave-shaped track while rotating. At this time, the moving mechanism 8 will also move along the two guide strips 6. The bottom of the moving mechanism 8 is provided between the bottom of the cavity of the support cylinder 3 and the fitting elastic component 9, which is slidingly installed with the support cylinder 3. When the moving mechanism 8 moves up and down, it will drive the fitting elastic component 9 to slide in the support cylinder 3. The fitting elastic component 9 has elastic properties, which can ensure that the moving mechanism 8 and the circular ring guide block 7 always maintain good contact. The bottom of the moving mechanism 8 is provided with a stirring mechanism 10 in the cavity of the bioreactor body 1. With the rotation and movement of the moving mechanism 8, the stirring mechanism 10 will stir the liquid in the bioreactor body 1. In addition, the moving mechanism 8 can also drive the stirring mechanism 10 to rotate while moving up and down, which makes the stirring mechanism 10 present a working state of rotating and moving up and down in the bioreactor body 1. It can make the liquid with higher temperature at the bottom of the bioreactor body 1 flow to the upper area, and at the same time make the liquid with lower temperature at the top of the bioreactor body 1 flow to the area with higher temperature, so as to break the temperature gradient at different heights in the bioreactor body 1. Even the area far away from the heating plate 11 can also achieve uniform temperature distribution, thereby improving the consistency of the temperature in the bioreactor, which is conducive to the biological process of cell culture and fermentation. In addition, the sealing cover 2 is used to seal the bioreactor body 1, and the two are connected by bolts to prevent foreign matter from entering and prevent leakage of gas or liquid during the reaction, so as to ensure that the reaction is carried out in a relatively stable and clean environment.

[0033] In one embodiment, for the above-mentioned driving mechanism 5, the driving mechanism 5 comprises a driving motor 501 fixedly installed at the top of the support cylinder 3, an output shaft 502 fixedly installed at the output end of the driving motor 501, and two guide bars 6 fixedly installed on the outer wall of the output shaft 502.

[0034] The moving mechanism 8 comprises a connecting block 801 sleeved on the output shaft 502 and the two guide bars 6, and a moving rod 802 fixedly installed at the bottom of the connecting block 801.

[0035] A connecting rod 803 is fixedly installed at the top of the connecting block 801, a ball 804 is arranged at the top of the connecting rod 803, and the ball 804 is in close contact with the wavy shape of the circular ring guide block 7.

[0036] The fitting elastic assembly 9 comprises a fixed block 901 fixedly installed at the inner wall bottom of the support cylinder 3, a spring 902 fixedly installed at the top of the fixed block 901, and a circular block 903 fixedly installed at the other end of the spring 902.

[0037] A plurality of sliding blocks 904 are fixedly installed on the outer wall of the circular block 903, the plurality of sliding blocks 904 are slidingly installed on the support cylinder 3, the circular block 903 is located at the bottom of the connecting block 801, and the spring 902 is sleeved on the moving rod 802.

[0038] When the liquid in the bioreactor body 1 needs to be stirred, the driving motor 501 is started, and the output shaft 502 at the output end rotates. Since the two guide strips 6 are fixedly installed on the outer wall of the output shaft 502, the guide strips 6 will rotate with the output shaft 502. The connecting block 801 is sleeved on the output shaft 502 and the two guide strips 6, so when the output shaft 502 and the guide strips 6 rotate, they will drive the connecting block 801 to rotate circumferentially. The connecting block 801 is provided with a ball 804 on the top through a connecting rod 803, and the ball 804 is in close contact with the wavy bottom of the annular guide block 7. During the rotation of the connecting block 801, due to the wavy structure of the bottom of the annular guide block 7, the ball 804 will roll along the wavy track of the annular guide block 7, which makes the connecting block 801 not only rotate circumferentially, but also move up and down along the two guide strips 6. The moving rod 802 fixedly installed at the bottom of the connecting block 801 will also move up and down accordingly. The fixed block 901 is fixedly installed at the bottom of the inner wall of the supporting barrel 3, and the other end of the spring 902 at the top of the fixed block 901 is connected to the circular block 903, and the spring 902 is sleeved on the moving rod 802. A plurality of sliding blocks 904 on the outer wall of the circular block 903 are slidingly installed on the supporting barrel 3. When the connecting block 801 moves up and down, it will generate a force on the circular block 903, causing the circular block 903 to slide in the supporting barrel 3 through the sliding blocks 904. The elastic property of the spring 902 ensures that the ball 804 on the connecting block 801 is always in close contact with the wavy bottom of the annular guide block 7, ensuring the stability of the entire mechanism.

[0039] In one embodiment, for the above-mentioned stirring mechanism 10, the stirring mechanism 10 comprises a stirring shaft 1001 fixedly installed at the bottom of the moving rod 802, and a plurality of stirring discs 1002 are installed on the circumferential surface of the stirring shaft 1001.

[0040] A plurality of stirring plates 1003 are circumferentially arranged on the outer wall of each of the plurality of stirring discs 1002, and the plurality of stirring plates 1003 are all arranged obliquely.

[0041] The stirring shaft 1001 is fixedly installed at the bottom of the moving rod 802, so that the stirring shaft 1001 rotates in a circle and moves up and down in the bioreactor body 1. A plurality of stirring discs 1002 are installed on the circumferential surface of the stirring shaft 1001, and move together with the stirring shaft 1001. In the rotating process of the stirring disc 1002, the stirring disc 1002 directly pushes the surrounding liquid to generate flow in the circumferential direction. In addition, because the stirring shaft 1001 moves up and down, the stirring disc 1002 also stirs the liquid at different height positions, which promotes the mixing of the liquid in the vertical direction. A plurality of inclined stirring plates 1003 are arranged in an array on the outer wall of each stirring disc 1002. When the stirring shaft 1001 drives the stirring disc 1002 to rotate, the inclined stirring plates 1003 generate an oblique thrust on the liquid, which further enhances the mixing of the liquid in the circumferential direction and guides the liquid to flow in the vertical direction. The synergistic effect of the plurality of stirring plates 1003 and the plurality of stirring discs 1002 rotating while moving up and down enables the liquid in the bioreactor body 1 to be fully mixed. The liquid at the lower part of the reactor, which has a higher temperature, is brought to the upper part by the stirring action, and the liquid at the upper part, which has a lower temperature, is brought to the lower part, thereby breaking the temperature gradient at different height positions in the bioreactor body 1. Even the area far from the heating plate 11 can achieve uniform temperature distribution through sufficient mixing of the liquid, which greatly improves the consistency of the temperature in the bioreactor.

[0042] In one embodiment, the bottom of the heating plate 11 is provided with a base 12.

[0043] The base 12 supports the heating plate 11 and the entire reactor.

[0044] In the description of the present specification, the description of the terms "one embodiment", "example", "specific example" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the utility model. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0045] The preferred embodiments of the utility model disclosed above are only used for illustrating the utility model. The preferred embodiments do not describe all the details, and the utility model is not limited to the specific embodiments described. Obviously, according to the content of the description, many modifications and changes can be made. The description selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that the persons skilled in the art can well understand and utilize the utility model. The utility model is limited only by the claims and the entire scope and equivalents thereof.

Claims

1. A tabletop glass bioreactor, comprising a bioreactor body (1), wherein a sealing cap (2) is provided on the top of the bioreactor body (1), a support cylinder (3) is fixedly installed on the top of the sealing cap (2), and a temperature probe (4) is provided on the top of the sealing cap (2), the probe of the temperature probe (4) extending into the inner cavity of the bioreactor body (1), characterized in that: The top of the support cylinder (3) is provided with a driving mechanism (5), symmetrical guide bars (6) are installed on the driving mechanism (5), a circular ring guide block (7) is fixedly installed in the inner cavity of the support cylinder (3), a moving mechanism (8) is installed on the output end of the two guide bars (6) and the driving mechanism (5), the top end of the moving mechanism (8) is close to the bottom end of the circular ring guide block (7), and the bottom of the circular ring guide block (7) is arranged in a wave shape close to the end of the moving mechanism (8), a close elastic assembly (9) is arranged between the bottom of the moving mechanism (8) and the bottom of the inner cavity of the support cylinder (3), the close elastic assembly (9) is slidingly installed on the support cylinder (3), a stirring mechanism (10) is installed at the bottom of the moving mechanism (8) in the inner cavity of the bioreactor body (1), and a heating plate (11) is installed at the bottom of the bioreactor body (1).

2. A benchtop glass bioreactor according to claim 1, wherein, The driving mechanism (5) comprises a driving motor (501), the driving motor (501) is fixedly installed at the top of the support cylinder (3), and an output shaft (502) is fixedly installed at the output end of the driving motor (501); the two guide bars (6) are fixedly installed on the outer wall of the output shaft (502).

3. A benchtop glass bioreactor according to claim 2, wherein, The moving mechanism (8) comprises a connecting block (801), the connecting block (801) is sleeved on the output shaft (502) and the two guide bars (6), and a moving rod (802) is fixedly installed at the bottom of the connecting block (801).

4. A benchtop glass bioreactor according to claim 3, wherein, A connecting rod (803) is fixedly installed at the top of the connecting block (801), a ball (804) is arranged at the top of the connecting rod (803), and the ball (804) is close to the wave shape of the circular ring guide block (7).

5. A benchtop glass bioreactor according to claim 4, wherein, The close elastic assembly (9) comprises a fixed block (901), the fixed block (901) is fixedly installed at the bottom of the inner wall of the support cylinder (3), a spring (902) is fixedly installed at the top of the fixed block (901), and a circular block (903) is fixedly installed at the other end of the spring (902).

6. A benchtop glass bioreactor according to claim 5, wherein, A plurality of sliding blocks (904) are fixedly installed on the outer wall of the circular block (903), the plurality of sliding blocks (904) are slidingly installed on the support cylinder (3), the circular block (903) is located at the bottom of the connecting block (801), and the spring (902) is sleeved on the moving rod (802).

7. A benchtop glass bioreactor according to claim 6, wherein, The stirring mechanism (10) comprises a stirring shaft (1001), the stirring shaft (1001) is fixedly installed at the bottom of the moving rod (802), and a plurality of stirring discs (1002) are installed on the circumferential surface of the stirring shaft (1001).

8. A benchtop glass bioreactor according to claim 7, wherein, A plurality of stirring plates (1003) are arranged in a circumferential array on the outer wall of the plurality of stirring discs (1002), and the plurality of stirring plates (1003) are all arranged in an inclined manner.

9. The benchtop glass bioreactor of claim 1, wherein, The bottom of the heating plate (11) is provided with a base (12).

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