Reagent containers, their operation methods, and reagent handling systems

Abstract

The present invention relates to a reagent container, a method for operating the same, and a reagent handling system. The reagent container includes a tube body and an elastic tube plug, the tube body includes a tube plug receiving section, the tube plug receiving section includes a first inner diameter section and a second inner diameter section in the depth direction of the tube body, the inner diameter of the second inner diameter section is smaller than the inner diameter of the first inner diameter section, and the elastic tube plug has a through channel in the direction of extension into the tube body, the elastic tube plug can be in a non-interference fit with the first inner diameter section or can be engaged with the first inner diameter section by a first interference fit scale, and the elastic tube plug can be in a second interference fit with the second inner diameter section by a second interference fit scale. When the elastic plug can be fitted with the first inner diameter section with a first interference fit scale, the second interference fit scale is greater than zero when the elastic plug can be brought into a non-interference fit state with the first inner diameter section, and when the elastic plug can be fitted with the first inner diameter section with a first interference fit scale, the second interference fit scale is greater than the first interference fit scale, and the through channel is formed to be compressed and sealed with radial contraction of the elastic plug when the elastic plug is fitted with the second inner diameter section with the second interference fit scale.

Description

Technical Field

[0001] Cross - reference to Related Applications This disclosure is based on Chinese applications with application numbers 202110023377.9 and 202120045981.7 filed on January 8, 2021, and claims the priority thereof. The disclosure content of these applications is incorporated into this disclosure by reference in its entirety.

[0002] Technical Field This disclosure relates to the technical field of biological detection, specifically to reagent containers, their operating methods, and systems for handling reagents.

Background Art

[0003] Polymerase chain reaction (PCR) is a molecular biology technique that can be used to amplify specific DNA fragments. The method can efficiently amplify selective gene fragments in vitro and then achieve the detection of target genes.

Summary of the Invention

Problems to be Solved by the Invention

[0004] During the operation of nucleic acid amplification, it may be necessary to add or remove a part of the formulation. At this time, it is necessary to lift the lid of the reagent container by hand and then close the lid by hand after adding or removing the reagent.

Means for Solving the Problems

[0005] Content of the Invention This disclosure provides a novel reagent container, and the novel structure of the reagent container makes it particularly suitable for automatic operation.

[0006] In some aspects, this disclosure is a reagent container, A pipe body including a pipe plug housing section, wherein the pipe plug housing section includes a first inner diameter section and a second inner diameter section along the depth direction of the pipe body, and the inner diameter of the second inner diameter section is smaller than the inner diameter of the first inner diameter section, An elastic pipe plug, wherein the elastic pipe plug has a through channel along the direction of entry into the pipe body. Includes, The elastic pipe plug can be in a non-tightened fit state with respect to the first inner diameter section, or it can have a first tight fit amount with respect to the first inner diameter section, and the elastic pipe plug can have a second tight fit amount with respect to the second inner diameter section. When the elastic pipe plug can be in a non-tightened fit state with respect to the first inner diameter section, the second tight fit amount is greater than zero, and when the elastic pipe plug can have a first tight fit amount with respect to the first inner diameter section, the second tight fit amount is greater than the first tight fit amount. The through channel is formed such that when the elastic plug has a second amount of tight fit with the second inner diameter section, it is compressed and sealed by the radial contraction of the elastic plug. The reagent container is disclosed.

[0007] In some embodiments, both the first and second interference fit amounts are greater than zero.

[0008] In some embodiments, a "non-interference fit" (where the amount of interference is greater than or equal to zero) is an intermediate fit (where the amount of interference is equal to zero / the amount of clearance is equal to zero) or a clearance fit (where the amount of clearance is greater than zero).

[0009] In some embodiments, “the through-channel is compressed and sealed” means that at least a portion or all of the through-channel is compressed and sealed.

[0010] In some embodiments, the through-channel is either open or spontaneously closed in a non-tight fit state. “Spontaneously closed” means that the through-channel remains closed only under the elastic pressure of the elastic plug itself when no radial pressure is applied to the elastic plug. When the through-channel is spontaneously closed, it can be penetrated by a pipette.

[0011] In some embodiments, the inner diameter of the first inner diameter section is greater than or equal to the outer diameter of at least one of the elastic stoppers.

[0012] In some embodiments, the inner diameter of the second inner diameter section is smaller than the outer diameter of at least one of the elastic pipe plugs.

[0013] In some embodiments, the elastic pipe plug is either in a non-compressed state with respect to the first inner diameter section or has a first amount of compression with respect to the first inner diameter section at a first insertion depth into the pipe body, and has a second amount of compression with respect to the second inner diameter section at a second insertion depth into the pipe body, with the second depth being greater than the first depth.

[0014] In some embodiments, the first inner diameter section and the second inner diameter section are directly adjacent to each other.

[0015] In some embodiments, a chamfered surface is provided between the first inner diameter section and the second inner diameter section.

[0016] In some embodiments, the inner diameter of the first inner diameter section decreases at a position adjacent to the second inner diameter section.

[0017] In some embodiments, the inner diameter of the second inner diameter section increases progressively at a position adjacent to the first inner diameter section.

[0018] In some embodiments, a smooth inner diameter transition section is provided at the joint between the first inner diameter section and the second inner diameter section.

[0019] In some embodiments, the second inner diameter section is deeper within the pipe body than the first inner diameter section.

[0020] In some embodiments, the pipe body and / or the elastic pipe plug are provided with a position limiting mechanism, and the position limiting mechanism prevents the elastic pipe plug from rising and / or falling with respect to the pipe body along the depth direction.

[0021] In some embodiments, the pipe body and / or the elastic pipe plug have the following position limiting mechanism, that is, - A first position limiting mechanism that prevents the elastic pipe plug from exiting the nozzle of the pipe body, - A second position limiting mechanism that prevents the elastic pipe plug from entering from the first inner diameter section into the second inner diameter section, - A third position limiting mechanism that prevents the elastic pipe plug from reaching a depth exceeding the plug accommodation section, and includes one or more of the above position limiting mechanisms.

[0022] In some aspects, the present disclosure is a reagent container, a pipe body including a plug accommodation section, an elastic pipe plug, the elastic pipe plug including a third outer diameter section and a fourth outer diameter section in the order in which the elastic pipe plug enters the plug accommodation section, the outer diameter of the fourth outer diameter section being larger than the outer diameter of the third outer diameter section, and the elastic pipe plug having a through channel along the entry direction into the pipe body. including [[ID=2​​When the third outer diameter section can be in a non-tight fit state with the pipe plug housing section, the second tight fit amount is greater than zero, and when the third outer diameter section can have a first tight fit amount with the pipe plug housing section, the second tight fit amount is greater than the first tight fit amount. When the fourth outer diameter section has a second interlocking fit with the plug housing section, the through channel is formed to be compressed and sealed by the radial contraction of the fourth outer diameter section. We provide reagent containers.

[0023] In some embodiments, the inner diameter of at least one of the plug housing sections is greater than or equal to the outer diameter of the third outer diameter section.

[0024] In some embodiments, the inner diameter of at least one of the plug housing sections is smaller than the outer diameter of the fourth outer diameter section.

[0025] In some embodiments, at a third depth in which the elastic plug enters the pipe body, the third outer diameter section is either in a non-tightened fit with the plug housing section or has a first tight fit with the plug housing section; at a fourth depth in which the elastic plug enters the pipe body, the fourth outer diameter section has a second tight fit with the plug housing section, and the fourth depth is greater than the third depth.

[0026] In some embodiments, at a first depth in which the elastic plug enters the pipe body, the third outer diameter section is either in a non-tightened fit with the plug housing section or has a first tight fit with the plug housing section; at a second depth in which the elastic plug enters the pipe body, the fourth outer diameter section has a second tight fit with the plug housing section, and the second depth is greater than the first depth.

[0027] In some embodiments, the average outer diameter of the third outer diameter section is smaller than the average outer diameter of the fourth outer diameter section.

[0028] In some embodiments, the third outer diameter section and the fourth outer diameter section are directly adjacent to each other.

[0029] In some embodiments, a chamfered surface is provided between the third outer diameter section and the fourth outer diameter section.

[0030] In some embodiments, the outer diameter of the third outer diameter section increases progressively at a position adjacent to the fourth outer diameter section.

[0031] In some embodiments, the outer diameter of the fourth outer diameter section decreases at a position adjacent to the third outer diameter section.

[0032] In some embodiments, the pipe body and / or the elastic plug are provided with a position limiting mechanism that prevents the elastic plug from moving up and / or down relative to the pipe body along the depth direction.

[0033] In some embodiments, the position limiting mechanism is as follows: - A first position limiting mechanism that prevents the elastic pipe stopper from coming out of the nozzle of the pipe body, - A third position limiting mechanism that prevents the elastic plug from reaching a depth exceeding the plug housing section. - A fourth position limiting mechanism that prevents the fourth outer diameter section of the pipe body from entering the plug housing section. This includes one or more of the following.

[0034] The reagent container is formed such that when the elastic stopper penetrates the pipe body to a first depth, the elastic stopper is in a non-tight fit state with respect to the stopper housing section, or has a first tight fit with respect to the stopper housing section, and the through channel is not compressed to form a seal state, or is compressed to form a first seal state, and when the elastic stopper penetrates the pipe body to a second depth, the elastic stopper has a second tight fit with respect to the stopper housing section, and the through channel is compressed to have a second seal state, wherein the second depth is greater than the first depth, and the degree of sealing in the second seal state of the through channel is greater than the degree of sealing in the first seal state.

[0035] In some embodiments, the second sealing state is an airtight seal.

[0036] In some embodiments, the through-channel includes a second channel section and a first channel section, according to the order in which the elastic plug enters the pipe body.

[0037] In some embodiments, the cross-sectional area of ​​the first channel section is larger than the cross-sectional area of ​​the second channel section.

[0038] In some embodiments, the channel cross-sectional shape of the first channel section is two-dimensional, and the channel cross-sectional shape of the second channel section is one-dimensional.

[0039] In some embodiments, the elastic plug further includes a sealing element, which is used to seal the through channel.

[0040] In some embodiments, the sealing element is removable.

[0041] In some embodiments, the sealing element is punctureable.

[0042] In some embodiments, the sample container described in any one of the above items is a nucleic acid detection reaction tube.

[0043] In some embodiments, the sample container described in any one of the above items is a PCR tube.

[0044] In some embodiments, the present disclosure relates to a method for handling a reagent container, comprising the following steps: a) Prepare a reagent container as described in any one of the above items, wherein the elastic stopper of the reagent container is fitted to the first inner diameter section, but is not yet tightly fitted to the second inner diameter section. b) Inject the reagent into the tube body by passing it through the through-channel of the elastic tube stopper using a pipette, and / or pipette the reagent from the tube body. c) Push the elastic pipe stopper so that it moves along the depth direction of the pipe body so that the elastic pipe stopper is tightly fitted with the second inner diameter section. Includes steps, Preferably, d) Place the reagent container in a pre-configured environment so that the reagents in the container can undergo reactions such as those related to nucleic acid amplification or nucleic acid detection. This further includes, Preferably, the method for handling the reagent container is a nucleic acid amplification method or a nucleic acid detection method. This invention provides a method for handling reagent containers.

[0045] In some embodiments, the present disclosure relates to a method for handling a reagent container, comprising the following steps: a) Prepare a reagent container as described in any one of the above items, wherein the third outer diameter section of the elastic stopper of the reagent container is fitted with the stopper housing section, but the fourth outer diameter section is not yet tightly fitted with the stopper housing section. b) Inject the reagent into the tube body by passing it through the through-channel of the elastic tube stopper using a pipette, and / or pipette the reagent from the tube body. c) Push the elastic pipe plug into the deeper part of the pipe body so that the fourth outer diameter section is tightly fitted with the pipe plug housing section. Includes steps, Preferably, d) Place the reagent container in a pre-configured environment so that the reagents in the container can undergo reactions such as those related to nucleic acid amplification or nucleic acid detection. This further includes, Preferably, the method for handling the reagent container is a nucleic acid amplification method or a nucleic acid detection method. This invention provides a method for handling reagent containers.

[0046] In some embodiments, the method of manipulating the reagent container is a method of sealing the nucleic acid detection reaction tube (for example, sealing it multiple times).

[0047] In some embodiments, the present disclosure is a system for handling reagents, - A reagent container as described in any one of the above items, - A pipette formed to inject a reagent into the tube body and / or pipette a reagent from the tube body by passing through the through-channel of the elastic tube stopper, - A pusher formed to press the elastic pipe stopper so as to move along the depth direction of the pipe body, Includes, Preferably, the system for handling the reagent further includes a manipulator to which a pipette and / or pusher is attached. We provide a system for handling reagents.

[0048] In some embodiments, the first inner diameter section or the second inner diameter section consists of a section of the pipe body, and the change in the inner diameter of the section along the depth direction of the pipe body is ±3% or less, for example ±2% or less, for example ±1% or less.

[0049] In some embodiments, the third outer diameter section or the fourth outer diameter section consists of the section of the elastic pipe stopper, and the change in the outer diameter of the section along the direction of entry into the pipe body is ±3% or less, for example ±2% or less, for example ±1% or less.

[0050] In some embodiments, the first channel section or the second channel section consists of a section of the through-channel of the elastic plug, and the change in the inner diameter of the section along the direction in which the elastic plug enters the pipe body is ±3% or less, for example ±2% or less, for example ±1% or less.

[0051] The first inner diameter section is adjacent to the second inner diameter section.

[0052] In some embodiments, the third outer diameter section is adjacent to the fourth outer diameter section.

[0053] In some embodiments, the first channel section is adjacent to the second channel section.

[0054] In some embodiments, the first inner diameter section and the second inner diameter section do not overlap.

[0055] In some embodiments, the third outer diameter section and the fourth outer diameter section do not overlap.

[0056] In some embodiments, the first channel division and the second channel division do not overlap.

[0057] In some embodiments, the inner diameter refers to the average inner diameter.

[0058] In some embodiments, the outer diameter refers to the average outer diameter.

[0059] Explanation of terms When the following terms are used in this invention, they may have the following meanings.

[0060] Various relative terms such as "front," "rear," "top," and "bottom," "up," "down," "above," and "below" may be used to facilitate the description of various embodiments. Relative terms are defined in relation to the conventional orientation of the structure, but do not necessarily indicate the actual orientation during the manufacture or use of the structure.

[0061] As used in the specification and attached claims, the singular forms "a," "an," and "the" include plural referents unless the context clearly indicates otherwise.

[0062] The term "container" means a vessel suitable for receiving, storing, transporting, and / or discharging contents, such as samples (e.g., blood, urine, serum, plasma, or liquefied biopsy samples), reagents (e.g., reagents for immunochemical tests, clinical chemistry tests, coagulation tests, hematological tests, molecular biological tests, etc.), or combinations thereof.

[0063] The term "tube body" may refer to a container having a cylindrical, conical, or rectangular shape. A tube body may have a closed bottom and an open top. The closed bottom of a cylindrical container may be rounded. A non-limiting example of a single cylindrical or conical separator is a primary or secondary tube known in the art. Alternatively, two or more tube bodies may be arranged as a multi-tube assembly. A non-limiting example of such a multi-tube assembly is a perforated plate well known in the art.

[0064] The term "internal diameter" refers to the diameter in the case of a circular cross-section, or the diameter of a circle with the same area in the case of a non-circular cross-section.

[0065] The term "outer diameter" refers to the diameter in the case of a circular cross-section, or the diameter of a circle with the same area in the case of a non-circular cross-section.

[0066] The term "through channel" may refer to a through hole or through slit. The cross-section of a through channel may be two-dimensional, e.g., circular, polygonal, or one-dimensional (e.g., a slit). The one-dimensional shape may be a "-" shape, a "+" shape, a "z" shape, an "*" shape, etc.

[0067] The term "elastic" can refer to the property of returning to its original size and shape after deformation. An elastic pipe stopper has an elastic modulus (Young's modulus) of 10. 4 ~10 8 Pa, for example 10 5 ~10 7 Pa, for example 10 6 ~10 7 The material may be Pa. The material of the elastic pipe stopper may be an elastic polymer, such as rubber or silicone rubber.

[0068] The "position limiting mechanism" may consist of a convex block, a stopper, a convex plate, a convex ring, a mosaic structure of irregularities between the elastic pipe plug and the pipe body, and a clamp structure between the elastic pipe plug and the pipe body.

[0069] The depth at a specific location within the pipe body refers to the distance from that location to the pipe nozzle. For example, "depth of the elastic pipe plug" refers to the distance from the bottom end of the elastic pipe plug to the pipe nozzle. For example, "depth of the first inner diameter section" refers to the distance from the lower edge of the first inner diameter section to the pipe nozzle.

[0070] Unless otherwise specified, the term "depth direction" refers to the distance from the nozzle to the bottom of the pipe.

[0071] The term "tight fit" refers to the excess size ΔD = D1 - D2 of the elastic stopper (ΔD) when the radial size (D1) of the elastic stopper is compared with the size (D2) of the pipe body in a tight fit with the elastic stopper, with the maximum ΔD taking precedence. The degree of interference of the tight fit (i.e., ΔD / D2) is, for example, greater than 0.01%, greater than 0.1%, greater than 0.5%, greater than 1%, greater than >3%, greater than 5%, greater than >10%, greater than 20%. The size D1 mentioned above may be the outer diameter of the elastic stopper, and the size D2 may be the inner diameter of the pipe body.

[0072] The term "tight fit" can refer to a situation where interference exists between mating parts or between mating parts. Tight fits have a negative tolerance.

[0073] "Tight fit," "friction fit," and "press-fit" are technical terms used interchangeably within this specification and may mean the intentional induction, increase, and / or utilization of friction / pressure. In a tight fit, the outside of the elastic plug is compressed against the inside of the pipe body to form a circumferential seal.

[0074] The term "pipette" refers to a sharp device that can penetrate a through-channel for injecting / extracting reagents.

[0075] The term "pusher" refers to an element that can apply pressure to an elastic pipe stopper.

[0076] The term "manipulator" refers to a device capable of moving objects. Motion is possible in any direction in three-dimensional space, for example, horizontally or vertically.

[0077] The term "nucleic acid amplification" broadly refers to techniques for increasing the copy number of nucleic acid molecules in a sample. The techniques used for nucleic acid amplification are well known in the field. One example of nucleic acid amplification is polymerase chain reaction (PCR). In polymerase chain reaction, a nucleic acid sample collected from a subject is brought into contact with a primer (the nucleic acid sample may be single-stranded or double-stranded; if the nucleic acid is double-stranded, the double strand is first dissociated, then annealed, and then brought into contact with the primer). The primer is then extended under appropriate conditions, and the steps of dissociation (denaturation), annealing, and extension are repeated, thereby amplifying the copy number of nucleic acid. Other examples of in vitro amplification techniques include strand displacement amplification, transcription-free isothermal amplification, repair chain reaction amplification, ligase chain reaction, gap-filling ligase chain reaction amplification, binding ligase detection PCR, and RNA non-transcription amplification.

[0078] The term "nucleic acid" broadly refers to a macromolecular form of nucleotide (deoxyribonucleotide (dNTPs) or ribonucleotide (rNTPs) or analogue thereof) of any length. Nucleic acids can have any three-dimensional structure and can perform any known or unknown functions. In relation to the inventions to which this application relates, non-limiting examples of nucleic acids include DNA, RNA, coding or non-coding regions of genes or gene fragments, one or more gene loci determined by linkage analysis, exons, introns, messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), small interfering RNA (siRNA), short hairpin RNA (shRNA), microRNA (miRNA), ribozymes, cDNA, recombinant nucleic acids, and branched nucleic acids. The nucleic acid comprises a plasmid, a vector, isolated DNA of any sequence, isolated RNA of any sequence, a nucleic acid probe, and a primer. The nucleic acid may contain one or more modified nucleotides, such as methylated nucleotides and nucleotide analogs. The nucleotide sequence of the nucleic acid may be interrupted by non-nucleotide components. The nucleic acid may be further modified after polymerization (for example, by linking or binding with a reporter).

[0079] The term "reagent" refers to any gaseous, liquid, or solid substance. A reagent may be a liquid. [Effects of the Invention]

[0080] Beneficial effects One or more technical solutions of this disclosure have one or more beneficial effects as described below. (1) By using a pipette to penetrate the through-channel of the elastic stopper, reagents can be added and removed without removing the elastic stopper from the tube body. (2) By pushing the elastic pipe plug deeply into the pipe body, compression and sealing of the through channel can be achieved. (3) Automatic operation becomes easier. (4) It has a simple structure. (5) It is low cost. [Brief explanation of the drawing]

[0081] [Figure 1] Figure 1 is a schematic diagram showing a reagent container in one example. [Figure 2] Figure 2 is a schematic diagram showing a reagent container in another embodiment. [Figure 3] Figure 3 is a schematic diagram showing a reagent container and elastic stopper in yet another embodiment. [Figure 4] Figure 4 is a schematic diagram showing a reagent container in yet another embodiment. [Figure 5] Figure 5 is a schematic diagram showing a reagent container in yet another embodiment. [Figure 6] Figure 6 is a schematic diagram showing a reagent handling system in one embodiment. [Modes for carrying out the invention]

[0082] A specific model for carrying out the present invention Embodiments of the present invention will be described in detail below with reference to examples, but as will be apparent to those skilled in the art, the following examples are for illustrative purposes only and should not be considered to limit the scope of the present invention. Unless specific conditions are indicated in the examples, the procedures were carried out under conventional conditions or conditions recommended by the manufacturer. Where reagents or equipment not indicated by the manufacturer were used, these were all commercially available conventional products.

[0083] Figure 1 shows a schematic diagram of the reagent container, where (a) and (b) show schematic diagrams of the tube body at different depths, respectively. As shown in Figures 1(a) and 1(b), the reagent container includes a tube body 10 and an elastic stopper 20, the tube body 10 includes a stopper housing section 16, the stopper housing section 16 includes a first inner diameter section 11 and a second inner diameter section 12 along the depth direction of the tube body 10, the inner diameter of the second inner diameter section 12 is smaller than the inner diameter of the first inner diameter section 11, the elastic stopper 20 has a through channel 21 along the direction of entry into the tube body 10, the elastic stopper 20 can be in an uncompressed state with the first inner diameter section 11, the elastic stopper 20 can have a second compression fit with the second inner diameter section 12, the second compression fit is greater than zero, and when the elastic stopper 20 has a second compression fit with the second inner diameter section 12, the elastic stopper 20 is compressed radially and the through channel 21 is compressed and sealed.

[0084] As shown in Figure 1(a), the elastic stopper 20 is inserted into the tube body 10 to a first depth. The elastic stopper 20 is positioned within the first inner diameter section 11 and has not yet entered the second inner diameter section 12. The outer diameter of the elastic stopper 20 is smaller than the inner diameter of the first inner diameter section 11, and the relationship between the elastic stopper 20 and the first inner diameter section 11 is a non-recess fit (clear fit). The elastic stopper 20 is not compressed radially, and the through-channel 21 is not compressed or sealed. The through-channel 21 is open (in some embodiments, the through-channel 21 closes spontaneously). When the reagent container is in this state, the through-channel 21 of the elastic stopper 20 can be passed through with a pipette, and reagents can be injected into the tube body 10 and / or pipetted out of the tube body 10.

[0085] As shown in Figure 1(b), the depth to which the elastic stopper 20 enters the main body of the tube 10 is the second depth, and the second depth is greater than the first depth. A portion of the elastic stopper extends into the second inner diameter section 12. The outer diameter of the elastic stopper 20 is greater than the inner diameter of the second inner diameter section 12, and the relationship between the elastic stopper 20 and the second inner diameter section 12 is an interference fit. When the reagent container is in this state, the elastic stopper 20 is compressed radially, and the through-channel 21 is compressed and sealed. The reagent container is well sealed.

[0086] In some embodiments, a method for handling the reagent container is provided, wherein the method comprises the following steps, namely: a) The reagent container described above is prepared, and although the elastic tube stopper 20 of the reagent container is fitted with the first inner diameter section 11, it is not yet tightly fitted with the second inner diameter section 12. b) Inject the reagent into the tube body 10 by passing it through the through-channel 21 of the elastic tube stopper 20 using a pipette, and / or pipette the reagent out of the tube body 10. c) Push the elastic pipe stopper 20 so that it moves along the depth direction of the pipe body 10 (to the deeper part of the pipe body) so that the elastic pipe stopper 20 and the second inner diameter section 12 are tightly fitted together. Includes steps, Preferably, this method is d) Place the reagent container in a pre-configured environment so that the reagent inside the container undergoes a reaction such as a reaction related to nucleic acid amplification or nucleic acid detection. A method for handling reagent containers is provided, which further includes the following.

[0087] In some embodiments, the method of manipulating the reagent container is either a nucleic acid amplification method or a nucleic acid detection method.

[0088] In some embodiments, as shown in Figure 1, the elastic pipe plug 20 includes a cylindrical plug body having a constant outer diameter along the direction of entry into the pipe body.

[0089] In some embodiments, the first inner diameter section 11 and the second inner diameter section 12 are directly adjacent. In some embodiments, a chamfered surface is provided between the first inner diameter section 11 and the second inner diameter section 12. In some embodiments, the inner diameter of the first inner diameter section 11 decreases at a position adjacent to the second inner diameter section 12. In some embodiments, the inner diameter of the second inner diameter section 12 increases at a position adjacent to the first inner diameter section 11. In some embodiments, the inner diameter of the joint between the first inner diameter section 11 and the second inner diameter section 12 has a smooth transition. The elastic pipe plug 20 can switch more smoothly between the first inner diameter section 11 and the second inner diameter section 12.

[0090] In one embodiment, the second inner diameter section 12 is located deeper inside the pipe body 10 than the first inner diameter section 11. The second inner diameter section 12 may be adjacent to the first inner diameter section 11, but they do not overlap.

[0091] Figure 2 shows a reagent container equipped with a position limiting mechanism. As shown in Figure 2, the reagent container is equipped with a position limiting mechanism (13, 14, 15). The position limiting mechanism prevents the elastic stopper 20 from rising and / or descending relative to the tube body 10 along the depth direction.

[0092] In one embodiment, as shown in Figure 2, the tube body 10 is equipped with a first position limiting mechanism 13 (e.g., a limiting block or limiting ring), which prevents the elastic stopper 20 from coming out of the nozzle. During use of the reagent container, it may be necessary to inject reagents into the tube body 10 and / or pipette reagents out of the tube body by using a pipette to pass through the through-channel 21 of the elastic stopper 20. Since the elastic stopper 20 may come off the tube body 10 when the pipette is withdrawn from the through-channel 21, the first position limiting mechanism 13 can effectively prevent the elastic stopper 20 from coming off the tube body 10.

[0093] In one embodiment, as shown in Figure 2, the tube body 10 is equipped with a second position limiting mechanism 14 (e.g., a limiting block or limiting ring) that prevents the elastic stopper 20 from advancing from the first inner diameter section 11 to the second inner diameter section 12. During use of the reagent container, it may be necessary to inject reagents into the tube body 10 and / or pipette reagents from the tube body by using a pipette to pass through the through-channel 21 of the elastic stopper 20. When inserting the pipette, it is possible to push the elastic stopper 20 to a deeper part of the tube body 10, for example, to the second inner diameter section 12. The second position limiting mechanism 14 can effectively prevent the elastic stopper 20 from being pushed to the second inner diameter section 12.

[0094] In one embodiment, as shown in Figure 2, the tube body 10 is equipped with a third position limiting mechanism 15 (e.g., a limiting block or limiting ring). The third position limiting mechanism prevents the elastic stopper 20 from reaching a depth beyond the stopper housing section 16. During use of the reagent container, it may be necessary to push the elastic stopper 20 to a deeper portion of the tube body 10 so that the elastic stopper 20 and the second inner diameter section 12 are in a tight-fit state. During the process of pushing the elastic stopper 20, it may be possible to push the elastic stopper 20 to a deeper position beyond the stopper housing section 16. The third position limiting mechanism 15 can effectively prevent the elastic stopper 20 from being pushed to a position deeper than the stopper housing section 16.

[0095] Needless to say, all of the above-mentioned position-limiting mechanisms (e.g., the first position-limiting mechanism, the second position-limiting mechanism, and the third position-limiting mechanism) can be elastic position-limiting mechanisms. That is, when the applied stress exceeds the load of the position-limiting mechanism, the elastic pipe plug can break through the position-limiting mechanism.

[0096] Figure 3 shows a schematic diagram of yet another reagent container. (a) and (c) show a longitudinal section of the reagent container and a cross section of the elastic stopper, respectively, with the elastic stopper positioned within the first inner diameter section, while (b) and (d) show a longitudinal section of the reagent container and a cross section of the elastic stopper, respectively, with the elastic stopper positioned within the second inner diameter section. As shown in Figure 3, the through-channel 21 includes a second channel section 212 and a first channel section 211, according to the sequence in which the elastic stopper 20 is inserted into the tube body 10, and the cross-sectional area of ​​the first channel section 211 is larger than the cross-sectional area of ​​the second channel section 212. During use of the reagent container, it may be necessary to inject reagents into the tube body 10 and / or pipette reagents from the tube body by passing a pipette through the through-channel 21 of the elastic stopper 20. If the through-channel has a larger inner diameter, the pipette can pass through easily. In addition, during the use of the reagent container, after the required reagents are injected and pipetting is performed, it may be necessary for the elastic stopper 20 and the through-channel 21 to be tightly fitted together so that the elastic stopper 20 is compressed radially and the through-channel 21 is compressed and sealed. If the through-channel has a small inner diameter, the through-channel can be easily compressed and sealed. According to the above solution, the cross-sectional area of ​​the first channel section 211 of the through-channel 21 is larger, making it easier for a pipette to pass through, and the cross-sectional area of ​​the second channel section 212 is smaller, making it easier to compress and seal. This solution improves the usefulness and sealing of the reagent container.

[0097] As shown in Figure 3(a), the depth to which the elastic plug 20 enters the pipe body 10 is the first depth. The elastic plug 20 is positioned within the first inner diameter section 11 and has not yet entered the second inner diameter section 12. The outer diameter of the elastic plug 20 is smaller than the inner diameter of the first inner diameter section 11, and the relationship between the elastic plug 20 and the first inner diameter section 11 is a non-compression fit (clearance fit). The first channel section 211 and the second channel section 212 have not yet been compressed or sealed.

[0098] As shown in Figure 3(b), the depth to which the elastic stopper 20 enters the main tube body 10 is the second depth, and the second depth is greater than the first depth. A portion of the elastic stopper enters the second inner diameter section 12. The outer diameter of the elastic stopper 20 is greater than the inner diameter of the second inner diameter section 12, and the relationship between the elastic stopper 20 and the second inner diameter section 12 is an interference fit. When the reagent container is in this state, the elastic stopper 20 is compressed radially, and the second channel section 212 of the through-channel 21 is compressed and sealed. The reagent container is well sealed.

[0099] As shown in Figures 3(c) and 3(d), the cross-sectional shape of the first inner diameter section 211 is two-dimensional, for example, circular, and the cross-sectional shape of the second channel section 212 is one-dimensional, for example, a "-" shape. Due to the elasticity of the elastic stopper, the pipette can pass through the channel even if the cross-sectional shape of the second channel section 212 is one-dimensional. In this solution, the shape of the first channel section 211 is favorable for pipette passage, and the shape of the second channel section 212 is favorable for compression and sealing. This solution improves the usefulness and sealing performance of the reagent container.

[0100] Figure 4 shows a schematic diagram of yet another reagent container. As shown in this figure, the elastic stopper 20 of the reagent container is provided with a sealing element 25, which seals the through channel 21. The sealing element 25 is a punctureable sealing element. For unused reagent containers, the elastic stopper 20 can be pre-fitted to the first inner diameter section 11, and the sealing element 25 can then seal the through channel 21, thereby preventing impurities from entering the container. When it is necessary to inject reagents into the tube body 10 and / or pipette reagents from the tube body, the sealing element 25 can be punctured using a pipette.

[0101] Figure 5 shows a schematic diagram of yet another reagent container. As shown in this figure, the reagent container includes a tube body 10 and an elastic stopper 20. The tube body 10 includes a stopper housing section 16. The elastic stopper 20 includes a third outer diameter section 23 and a fourth outer diameter section 24 in the order of entry into the stopper housing section 16, with the outer diameter of the fourth outer diameter section 24 being larger than the outer diameter of the third outer diameter section 23. The elastic stopper 20 has a through channel 21 in the direction of entry into the tube body. The third outer diameter section 23 is in a non-tightened state with respect to the stopper housing section 16, and the fourth outer diameter section 24 has a second tightness with respect to the stopper housing section 16, and the second tightness is greater than zero. The elastic pipe plug 20 is formed such that when the fourth outer diameter section 24 has a second amount of tight fit with the pipe plug housing section 16, the fourth outer diameter section 24 is compressed radially, and the through channel 21 is compressed and sealed.

[0102] As shown in Figure 5(a), the depth to which the elastic stopper 20 enters the tube body 10 is the first depth. The third outer diameter section 23 of the elastic stopper 20 enters the stopper housing section 16, while the fourth outer diameter section 24 has not yet entered the stopper housing section 16. The outer diameter of the third outer diameter section 23 is smaller than the inner diameter of the stopper housing section 16, and the relationship between the stopper housing section 16 and the third outer diameter section 23 is a non-recessive fit (clearance fit). The third outer diameter section 23 is not compressed radially, and the through-channel 21 is not compressed or sealed. The through-channel 21 is open and can be passed through by a pipette. In this state, the reagent can be injected into the tube body 10 and / or pipetted out of the tube body 10 by passing the through-channel 21 of the elastic stopper 20 using a pipette.

[0103] As shown in Figure 5(b), the depth to which the elastic stopper 20 enters the pipe body 10 is the second depth, and the second depth is greater than the first depth. At least a portion of the fourth outer diameter section 24 of the elastic stopper enters the stopper housing section 16. The outer diameter of the fourth outer diameter section 24 is greater than the inner diameter of the stopper housing section 16, and the relationship between the fourth outer diameter section 24 and the stopper housing section 16 is an interference fit. The fourth outer diameter section 24 is compressed radially, and the through-channel 21 is compressed and sealed. In this state, the reagent container is well sealed.

[0104] In some embodiments, a method for handling the reagent container is provided, comprising the following steps: a) Prepare the reagent container in which the third outer diameter section 23 of the elastic stopper 20 of the reagent container is fitted with the stopper housing section 16, but the fourth outer diameter section 24 is not yet tightly fitted with the stopper housing section 16. b) Inject the reagent into the tube body 10 by passing it through the through-channel 21 of the elastic tube stopper 20 using a pipette, and / or pipette the reagent out of the tube body 10. c) Push the elastic pipe plug 20 to a deeper portion of the pipe body 10 so that the fourth outer diameter section 24 is tightly fitted with the pipe plug housing section 16. Includes steps, Preferably, the method for handling the reagent container is d) Place the reagent container in a pre-configured environment so that the reagent inside the container undergoes a reaction such as a reaction related to nucleic acid amplification or nucleic acid detection. This further includes, A method for handling reagent containers is provided.

[0105] In some embodiments, the method of manipulating the reagent container is either a nucleic acid amplification method or a nucleic acid detection method.

[0106] In some embodiments, as shown in Figure 5, the plug housing section 16 includes a cylindrical lumen with a constant inner diameter along the depth direction of the pipe body.

[0107] In some embodiments, as shown in Figure 5(a), the reagent container is equipped with a position limiting mechanism 17, which prevents the elastic stopper 20 from rising and / or falling relative to the tube body 10 in the depth direction. The position limiting mechanism 17 includes a groove provided in the third outer diameter section 23 and a convex block provided in the stopper housing section 16, and the groove and the convex block can be fitted and engaged. The position limiting mechanism 17 can prevent the elastic stopper 20 from coming out of the nozzle.

[0108] In some embodiments, the third outer diameter section 23 and the fourth outer diameter section 24 are directly adjacent. In some embodiments, a chamfered surface is provided between the third outer diameter section 23 and the fourth outer diameter section 24. In some embodiments, the outer diameter of the third outer diameter section 23 increases at a position adjacent to the fourth outer diameter section 24. In some embodiments, the outer diameter of the fourth outer diameter section 24 decreases at a position adjacent to the third outer diameter section 23. In the above solution, the fitting state between the elastic pipe plug 20 and the pipe plug housing section 16 can be switched more smoothly. In the above embodiment, the fitting relationship between the elastic pipe plug and the pipe plug housing section can be easily switched.

[0109] Figure 6 shows a schematic diagram of a reagent handling system. In some embodiments, the reagent handling system is such that the system is... - A reagent container comprising a tube body 10 and an elastic tube stopper 20, wherein the tube body 10 includes a tube stopper housing section 16, the tube stopper housing section 16 includes a first inner diameter section 11 and a second inner diameter section 12 along the depth direction of the tube body 10, the inner diameter of the second inner diameter section 12 is smaller than the inner diameter of the first inner diameter section 11, the elastic tube stopper 20 has a through channel 21 along the direction of entry into the tube body 10, the elastic tube stopper 20 can have a first compression fit with the first inner diameter section 11, the elastic tube stopper 20 can have a second compression fit with the second inner diameter section 12, and the second compression fit is larger than the first compression fit, and when the elastic tube stopper 20 has a second compression fit with the second inner diameter section 12, the elastic tube stopper 20 is compressed radially, and the through channel 21 is compressed and sealed, - A pipette 30 is formed to inject a reagent into the tube body 10 and / or pipette the reagent from the tube body 10 by passing through the through channel 21 of the elastic tube stopper 20, - A pusher 40 is formed to press the elastic pipe stopper 20 so as to move along the depth direction of the pipe body 10, A system for handling reagents, including the above, is provided.

[0110] The method for operating the system that handles the above reagents is as follows: a) Prepare a reagent container in which the elastic stopper 20 of the reagent container is fitted with the first inner diameter section 11, but is not yet tightly fitted with the second inner diameter section 12. b) Inject the reagent into the tube body 10 by passing it through the through-channel 21 of the elastic tube stopper 20 using a pipette, and / or pipette the reagent out of the tube body 10. c) Push the elastic pipe stopper 20 so that it moves along the depth direction of the pipe body 10 (to the deeper part of the pipe body) so that the elastic pipe stopper 20 and the second inner diameter section 12 are tightly fitted together. Includes steps, Preferably, a method for operating the system for handling the above reagents is: d) Place the reagent container in a pre-configured environment so that the reagents in the container can undergo reactions such as those related to nucleic acid amplification or nucleic acid detection. This further includes the following.

[0111] In some embodiments, as shown in Figure 6(a), the tube body 10 is equipped with a second position limiting mechanism 14. The second position limiting mechanism prevents the elastic stopper 20 from entering the second inner diameter section 12 from the first inner diameter section 11. The second position limiting mechanism 14 prevents the elastic stopper 20 from being carried to a deeper position as the pipette passes through the through-channel 21. As shown in Figure 6(b), the stress applied by the pusher 40 exceeds the load of the second position limiting mechanism 14, and the elastic stopper 20 breaks through the second position limiting mechanism 14 under the pressure of the pusher 40 and reaches the second inner diameter section 12.

[0112] In some embodiments, the reagent handling system further includes a manipulator 50 to which a pipette 30 and / or pusher 40 is attached.

[0113] While specific embodiments of the present invention have been described in detail, as will be apparent to those skilled in the art, various modifications and changes can be made in detail based on all disclosed teachings, and all such changes are included within the scope of protection of the present invention. The entire scope of the present invention is given by the appended claims and their equivalents. Furthermore, this disclosure includes the following inventions. The first aspect is, A reagent container, A pipe body (10) including a pipe plug housing section (16), wherein the pipe plug housing section (16) includes a first inner diameter section (11) and a second inner diameter section (12) along the depth direction of the pipe body (10), and the inner diameter of the second inner diameter section (12) is smaller than the inner diameter of the first inner diameter section (11), An elastic pipe plug (20), wherein the elastic pipe plug (20) is provided with a through channel (21) along the direction of entry into the pipe body (10). Includes, The elastic pipe plug (20) can be in a non-tightened fit state with the first inner diameter section (11), or it can have a first tight fit amount with the first inner diameter section (11), and the elastic pipe plug (20) can have a second tight fit amount with the second inner diameter section (12), When the elastic pipe plug can be in a non-tightened fit state with respect to the first inner diameter section, the second tight fit amount is greater than zero, and when the elastic pipe plug can have a first tight fit amount with respect to the first inner diameter section, the second tight fit amount is greater than the first tight fit amount. The through channel (21) is formed such that when the elastic plug (20) has a second amount of tight fit with the second inner diameter section (12), it is compressed and sealed by the radial contraction of the elastic plug (20). It is a reagent container. The second aspect is, The aforementioned reagent container has the following characteristics, namely, - The inner diameter of the first inner diameter section (11) is greater than or equal to the outer diameter of at least one of the elastic pipe plugs (20). - The inner diameter of the second inner diameter section (12) is smaller than the outer diameter of at least one of the elastic pipe plugs (20). - The elastic pipe stopper (20) is in a non-compressed state with the first inner diameter section (11) or has a first amount of compression with the first inner diameter section (11) at a first insertion depth into the pipe body (10), and has a second amount of compression with the second inner diameter section (12) at a second insertion depth into the pipe body (10), and the second depth is greater than the first depth. A reagent container according to the first embodiment, having one or more of the following: The third aspect is, The aforementioned reagent container has the following characteristics, namely, - The first inner diameter section (11) and the second inner diameter section (12) are directly adjacent to each other. - A chamfered surface is provided between the first inner diameter section (11) and the second inner diameter section (12). - The inner diameter of the first inner diameter section (11) decreases at a position adjacent to the second inner diameter section (12), - The inner diameter of the second inner diameter section (12) increases at a position adjacent to the first inner diameter section (11), - A smooth inner diameter transition section is provided at the joint between the first inner diameter section (11) and the second inner diameter section (12). - The second inner diameter section (12) is deeper than the first inner diameter section (11) within the pipe body. A reagent container according to the first embodiment, having one or more of the following: The fourth aspect is, The pipe body (10) and / or the elastic pipe plug (20) are equipped with a position limiting mechanism, which prevents the elastic pipe plug (20) from rising and / or falling relative to the pipe body (10) along the depth direction. Preferably, the pipe body (10) and / or the elastic pipe stopper (20) are subject to the following position limiting mechanism, namely, - A first position limiting mechanism (13) that prevents the elastic pipe stopper (20) from coming out of the nozzle of the pipe body (10), - A second position limiting mechanism (14) that prevents the elastic pipe stopper (20) from entering the second inner diameter section (12) from the first inner diameter section (11), - A third position limiting mechanism (15) that prevents the elastic pipe plug (20) from reaching a depth exceeding the pipe plug housing section (16), A reagent container in a first embodiment, comprising one or more position-limiting mechanisms. The fifth aspect is, A reagent container, Pipe body (10) including the pipe plug housing section (16), An elastic pipe plug (20), wherein the elastic pipe plug includes a third outer diameter section (23) and a fourth outer diameter section (24) in the order of entry into the pipe plug housing section (16), the outer diameter of the fourth outer diameter section (24) is larger than the outer diameter of the third outer diameter section (23), and the elastic pipe plug (20) is provided with a through channel (21) along the direction of entry into the pipe body. Includes, The third outer diameter section (23) can be in a non-tightened fit state with the plug housing section (16), or it can have a first tight fit amount with the plug housing section (16), and the fourth outer diameter section (24) can have a second tight fit amount with the plug housing section (16), When the third outer diameter section can be in a non-tight fit state with the pipe plug housing section, the second tight fit amount is greater than zero, and when the third outer diameter section can have a first tight fit amount with the pipe plug housing section, the second tight fit amount is greater than the first tight fit amount. The through channel (21) is formed such that when the fourth outer diameter section (24) has a second amount of tight fit with the plug housing section (16), it is compressed and sealed by the radial contraction of the fourth outer diameter section (24). It is a reagent container. The sixth aspect is, The aforementioned reagent container has the following characteristics, namely, - At least one inner diameter of the pipe plug housing section (16) is greater than or equal to the outer diameter of the third outer diameter section (23), - At least one inner diameter of the pipe plug housing section (16) is smaller than the outer diameter of the fourth outer diameter section (24), - At the first depth in which the elastic pipe plug (20) enters the pipe body (10), the third outer diameter section (23) is in a non-tightened fit state with the pipe plug housing section (16), or has the first amount of tight fit with the pipe plug housing section (16), and at the second depth in which the elastic pipe plug (20) enters the pipe body (10), the fourth outer diameter section (24) has the second amount of tight fit with the pipe plug housing section (16), and the second depth is greater than the first depth. A reagent container according to the fifth embodiment, having one or more of the above. The seventh aspect is, The aforementioned reagent container has the following characteristics, namely, - The third outer diameter section (23) and the fourth outer diameter section (24) are directly adjacent to each other. - A chamfered surface is provided between the third outer diameter section (23) and the fourth outer diameter section (24). - The outer diameter of the third outer diameter section (23) increases at a position adjacent to the fourth outer diameter section (24), - The outer diameter of the fourth outer diameter section (24) decreases at a position adjacent to the third outer diameter section (23), A reagent container according to the sixth embodiment, having one or more of the above. The eighth aspect is, The pipe body (10) and / or the elastic pipe stopper (20) are equipped with a position limiting mechanism, the position limiting mechanism prevents the elastic pipe stopper (20) from rising and / or falling relative to the pipe body (10) along the depth direction. Preferably, the position limiting mechanism is as follows: - A first position limiting mechanism (13) that prevents the elastic pipe stopper (20) from coming out of the nozzle of the pipe body (10), - A third position limiting mechanism (15) that prevents the elastic pipe plug (20) from reaching a depth exceeding the pipe plug housing section (16), - A fourth position limiting mechanism that prevents the fourth outer diameter section (24) of the pipe body (20) from entering the pipe plug housing section. A reagent container according to a fifth embodiment, comprising one or more of the following: The ninth aspect is, The reagent container is formed such that when the elastic stopper (20) penetrates the pipe body (10) to a first depth, the elastic stopper (20) is in a non-tightened fit state with the stopper housing section (16), or has a first tight fit amount with the stopper housing section (16), and the through channel (21) is not compressed to form a seal state, or is compressed to form a first seal state, and when the elastic stopper (20) penetrates the pipe body (10) to a second depth, the elastic stopper (20) is compressed to have a second tight fit amount with the stopper housing section (16), and the through channel (21) has a second seal state. The second depth is greater than the first depth, and the degree of sealing in the second sealed state of the through channel (21) is greater than the degree of sealing in the first sealed state. Preferably, the reagent container is one of the first to eighth embodiments, wherein the second sealing state is an airtight seal. The tenth aspect is, In the order in which the elastic pipe plug (20) enters the pipe body (10), the through channel (21) includes a second channel section (212) and a first channel section (211), and the channel sections have the following characteristics: - The cross-sectional area of ​​the first channel segment (211) is larger than the cross-sectional area of ​​the second channel segment (212), - The channel cross-sectional shape of the first channel section (211) is two-dimensional, and the channel cross-sectional shape of the second channel section (212) is one-dimensional. A reagent container according to any one of the first to eighth embodiments, having one or more of the above. The eleventh aspect is, The elastic plug (20) further includes a sealing element (25), which is used to seal the through channel (21). Preferably, the sealing element (25) is removable. Preferably, the reagent container is one of the first to eighth embodiments, wherein the sealing element (25) is punctureable. The twelfth aspect is, The reagent container is a PCR tube, according to any one of the first to eighth embodiments. The 13th aspect is, A method for handling reagent containers, comprising the following steps: a) Prepare a reagent container in any one of the first to twelfth embodiments, wherein the elastic stopper (20) of the reagent container is fitted with the first inner diameter section (11), but is not yet tightly fitted with the second inner diameter section (12), b) Inject the reagent into the tube body (10) and / or pipette the reagent out of the tube body (10) by passing it through the through channel (21) of the elastic tube stopper (20) using a pipette. c) The step of pushing the elastic pipe stopper (20) so that it moves along the depth direction of the pipe body (10) so that the elastic pipe stopper (20) is tightly fitted with the second inner diameter section (12), Preferably, d) Place the reagent container in a pre-configured environment so that the reagents in the container can undergo reactions such as those related to nucleic acid amplification or nucleic acid detection. This further includes, Preferably, the method for manipulating the reagent container is a nucleic acid amplification method or a nucleic acid detection method. The 14th aspect is, A method for handling reagent containers, comprising the following steps: a) Prepare a reagent container in any one of the first to twelfth embodiments, wherein the third outer diameter section (23) of the elastic stopper (20) of the reagent container is fitted with the stopper housing section (16), but the fourth outer diameter section (24) is not yet tightly fitted with the stopper housing section (16). b) Inject the reagent into the tube body (10) and / or pipette the reagent out of the tube body (10) by passing it through the through channel (21) of the elastic tube stopper (20) using a pipette. c) The process includes the step of pushing the elastic pipe plug (20) to a deeper portion of the pipe body (10) so that the fourth outer diameter section (24) is tightly fitted with the pipe plug housing section (16), Preferably, d) further comprising placing the reagent container in a pre-configured environment so as to allow the reagent in the container to undergo a reaction such as a reaction related to nucleic acid amplification or nucleic acid detection, Preferably, the method for manipulating the reagent container is a nucleic acid amplification method or a nucleic acid detection method. The 15th aspect is, A system for handling reagents, - A reagent container in any one of the first to twelfth embodiments, - A pipette formed to inject a reagent into the tube body (10) and / or pipette a reagent from the tube body (10) by passing through the through channel (21) of the elastic tube stopper (20), - A pusher formed to press the elastic pipe stopper (20) so as to move along the depth direction of the pipe body (10), Includes, Preferably, the system for handling the reagent further includes a manipulator to which the pipette and / or the pusher is attached. This is a system for handling reagents.

Claims

1. A reagent container, A pipe body (10) including a pipe plug housing section (16), wherein the pipe plug housing section (16) includes a first inner diameter section (11) and a second inner diameter section (12) along the depth direction of the pipe body (10), the inner diameter of the second inner diameter section (12) is smaller than the inner diameter of the first inner diameter section (11), and the second inner diameter section (12) is deeper within the pipe body than the first inner diameter section (11), An elastic pipe plug (20), wherein the elastic pipe plug (20) is provided with a through channel (21) along the direction of entry into the pipe body (10). Includes, In the order in which the elastic pipe plug (20) enters the pipe body (10), the through channel (21) includes a second channel section (212) and a first channel section (211). The cross-sectional area of ​​the first channel section (211) is larger than the cross-sectional area of ​​the second channel section (212), When the elastic stopper (20) enters the pipe body (10) to a first depth, the elastic stopper (20) is positioned within the first inner diameter section (11) and has not yet entered the second inner diameter section (12), the outer diameter of the elastic stopper (20) is smaller than the inner diameter of the first inner diameter section (11), the relationship between the elastic stopper (20) and the first inner diameter section (11) is a non-reinforcement fit or a gap fit, and the first channel section (211) and the second channel section (212) are not yet compressed and sealed. A reagent container in which, when the elastic stopper (20) enters the tube body (10) at a second depth greater than the first depth, a portion of the elastic stopper enters the second inner diameter section (12), the outer diameter of the elastic stopper (20) is greater than the inner diameter of the second inner diameter section (12), the relationship between the elastic stopper (20) and the second inner diameter section (12) is an interlocking fit, the elastic stopper (20) is compressed radially, and the second channel section (212) is compressed and sealed.

2. The aforementioned reagent container has the following characteristics, namely, - The first inner diameter section (11) and the second inner diameter section (12) are directly adjacent to each other. - A chamfered surface is provided between the first inner diameter section (11) and the second inner diameter section (12). - The inner diameter of the first inner diameter section (11) decreases at a position adjacent to the second inner diameter section (12). - The inner diameter of the second inner diameter section (12) increases at a position adjacent to the first inner diameter section (11), - A smooth inner diameter transition section is provided at the joint between the first inner diameter section (11) and the second inner diameter section (12). A reagent container according to claim 1, having one or more of the following:

3. The reagent container according to claim 1, wherein the tube body (10) and / or the elastic stopper (20) are equipped with a position limiting mechanism, the position limiting mechanism prevents the elastic stopper (20) from rising and / or descending along the depth direction of the tube body (10).

4. The pipe body (10) and / or the elastic pipe stopper (20) are provided with the following position limiting mechanism, namely, - A first position limiting mechanism (13) that prevents the elastic pipe stopper (20) from coming out of the nozzle of the pipe body (10), - A second position limiting mechanism (14) that prevents the elastic pipe stopper (20) from entering the second inner diameter section (12) from the first inner diameter section (11), - A third position limiting mechanism (15) that prevents the elastic pipe stopper (20) from reaching a depth exceeding the pipe stopper housing section (16), The reagent container according to claim 1, comprising one or more of the following position limiting mechanisms.

5. The reagent container according to claim 1, wherein the channel cross-sectional shape of the first channel section (211) is two-dimensional, and the channel cross-sectional shape of the second channel section (212) is one-dimensional.

6. The reagent container according to claim 1, wherein the elastic plug (20) further includes a sealing element (25), and the sealing element (25) is used to seal the through channel (21).

7. The reagent container according to claim 6, wherein the sealing element (25) is removable.

8. The reagent container according to claim 6, wherein the sealing element (25) is punctureable.

9. The reagent container according to claim 1, wherein the reagent container is a PCR tube.

10. A method for handling reagent containers, comprising the following steps: a) Prepare a reagent container according to any one of claims 1 to 9, wherein the elastic tube stopper (20) of the reagent container is fitted with the first inner diameter section (11), but is not yet tightly fitted with the second inner diameter section (12), b) Inject the reagent into the tube body (10) and / or pipette the reagent out of the tube body (10) by passing it through the through channel (21) of the elastic tube stopper (20) using a pipette. c) A method for operating a reagent container, comprising the step of pushing the elastic stopper (20) so that it moves along the depth direction of the tube body (10) so that the elastic stopper (20) is tightly fitted with the second inner diameter section (12).

11. The above method is d) The method according to claim 10, further comprising placing the reagent container in a pre-set environment so as to allow it to undergo the reaction.

12. The method according to claim 10, wherein the method for manipulating the reagent container is a nucleic acid amplification method or a nucleic acid detection method.

13. A system for handling reagents, - A reagent container according to any one of claims 1 to 9, - A pipette formed to inject a reagent into the tube body (10) and / or pipette a reagent from the tube body (10) by passing through the through channel (21) of the elastic tube stopper (20), - A pusher formed to press the elastic pipe stopper (20) so as to move along the depth direction of the pipe body (10), A system for handling reagents, including [specific reagents].

14. The system according to claim 13, wherein the system for handling the reagent further includes a manipulator to which the pipette and / or the pusher is attached.

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