A pharmaceutical composition for the prevention, mitigation, or treatment of cancer, containing 2,6-dichloro-4-(4-(4-hydroxycyclohexylamino)-7H-pyrrolo[2,3-D]pyrimidine-5-yl)phenol as an active ingredient.

Abstract

The present invention relates to a pharmaceutical composition for preventing or treating cancer, which contains 2,6-dichloro-4-(4-(4-hydroxycyclohexylamino)-7H-pyrrolo[2,3-D]pyrimidin-5-yl)phenol as an active ingredient. The present invention has been found to inhibit the formation of tumor-like masses in breast cancer cell lines, and to show effects that are more prominent than or equal to the anticancer drugs sorafenib and etoposide, which are topoisomerase inhibitors widely used in lung cancer, ovarian cancer, colon cancer, melanoma, etc. In addition, the compound of Example 1 according to the present invention shows synergistic anticancer effects when combined with radiation therapy or other anticancer drugs in breast cancer cell lines and liver cancer cell lines, and therefore can be developed as an anticancer drug or food that shows excellent effects in the treatment of cancer.

Description

[Technical Field]

[0001] Background of the present invention 1. Field of the present invention The present invention relates to a pharmaceutical composition for preventing or treating cancer, containing 2,6-dichloro-4-(4-(4-hydroxycyclohexylamino)-7H-pyrrolo[2,3-D]pyrimidine-5-yl)phenol as an active ingredient. [Background technology]

[0002] 2. Description of related technologies Despite the fact that cancer incidence is increasing with the advancement of civilization, the treatment of cancer patients still relies on surgery, radiation therapy, and chemotherapy with highly cytotoxic anticancer drugs. However, these treatments are generally limited to patients with early-stage cancer or specific types of cancer, and they cause various side effects. Therefore, there is a need to develop anticancer drugs that are effective with fewer side effects while having a good safety profile.

[0003] In particular, the breast cancer market is known to be an area where medical needs are not being met, due to a lack of high efficacy and safety in anti-cancer treatments.

[0004] Regarding breast cancer treatment, various therapies with different mechanisms targeting patients with varying disease characteristics, such as hormone therapy, chemotherapy, and targeted therapy, have been introduced. However, there is a certain clinical demand for therapies with superior efficacy and safety against cancer. Among these, triple-negative breast cancer (TNBC), which accounts for 16% of all breast cancers, is difficult to treat due to its poor prognosis after treatment, and there are not enough available therapies that target TNBC (Non-patent Literature 1, Rev Peru Med Exp Salud Publica. Oct-Dec 2013;30(4):649-56).

[0005] Currently, treatment for breast cancer includes therapies that inhibit the human epidermal growth factor receptor 2 (hereinafter referred to as "HER2") gene, which is involved in tumor growth, and anti-hormone drugs are widely used. However, in the case of TNBC, the HER2 receptor, estrogen receptor, and progesterone receptor are all negative, and therefore do not respond to existing anticancer drugs. Consequently, there is an urgent need for new targeted therapies that can treat the disease safely and efficiently.

[0006] Therefore, the inventors have researched and perfected the pharmaceutical composition of the present invention, which can be used as a safe and effective anticancer drug, using various cancer cell lines. [Overview of the project]

[0007] Summary of the present invention The object of the present invention is to provide a pharmaceutical composition for preventing or treating cancer. The object of the present invention is to provide a health functional food composition for preventing or treating cancer.

[0008] The object of the present invention is to provide a pharmaceutical kit for preventing or treating cancer. The object of the present invention is to provide a combination of radiation and anticancer drugs for the prevention or treatment of cancer.

[0009] Another object of the present invention is to provide a method for treating cancer, the method comprising the step of administering it to a subject in need. Another object of the present invention is to provide compounds for preventing or treating cancer. Another object of the present invention is to provide the use of compounds for the preparation of pharmaceuticals for use in the prevention or treatment of cancer.

[0010] To achieve the above objective, in aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating cancer, comprising as an active ingredient a compound represented by Formula 1 as described herein, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof. In another aspect of the present invention, the present invention provides a health functional food composition for preventing or treating cancer, comprising a compound represented by Formula 1 described herein as an active ingredient.

[0011] In another aspect of the present invention, the present invention provides a pharmaceutical kit for preventing or treating cancer, comprising: a first component containing a pharmaceutically effective amount of an anticancer agent; and a second component containing as an active ingredient a compound represented by Formula 1 described herein, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof. In another aspect of the present invention, the present invention provides a combination of radiation and anticancer agents for the prevention or treatment of cancer.

[0012] In another aspect of the present invention, the present invention provides a method for treating cancer, the method comprising the step of administering a compound represented by Formula 1 as described herein to a subject in need thereof. In another aspect of the present invention, the present invention provides a compound represented by Formula 1 as described herein for use in the prevention or treatment of cancer. In another aspect of the present invention, the present invention provides the use of a compound represented by Formula 1 described herein for the preparation of a pharmacopoeia for use in the prevention or treatment of cancer.

[0013] Favorable effects The compound of Example 1 of the present invention inhibits the formation of tumor-like masses in breast cancer cell lines, and when compared with the anticancer drugs sorafenib and etoposide, which are topoisomerase inhibitors widely used in lung cancer, ovarian cancer, colon cancer, melanoma, etc., it has been found to exhibit an effect significantly greater than or equal to that of the anticancer drugs sorafenib and etoposide. In addition, according to the present invention, the compound of Example 1 exhibits a synergistic anticancer effect when combined with radiotherapy or other anticancer drugs in breast cancer cell lines and liver cancer cell lines, and thus can be developed as an anticancer drug or food exhibiting excellent effects in the treatment of cancer.

Brief Description of the Drawings

[0014] Brief Description of the Drawings [Figure 1] Figure 1 is a chart showing the evaluation of the inhibitory ability of the expression of major proteins. [Figure 2] Figure 2 is a series of graphs showing the cell viability of the human-derived breast cancer cell line BT20 48 hours after treatment with the compound of Example 1 in culture. [Figure 3] Figure 3 is a series of graphs showing the cell viability of the TNBC cell line MDA-MB-231 48 hours after treatment with the compound of Example 1 in culture.

[0015] [Figure 4] Figure 4 is a series of graphs showing the cell viability of MDA-MB-231, MDA-MB-453, and BT-20 24 hours after treatment with the compound of Example 1. [Figure 5] Figure 5 is a graph confirming that treatment with the compound of Example 1 can significantly inhibit tumor growth. [Figure 6] Figure 6 is a series of photographs showing apoptotic bodies that have undergone the apoptosis process.

[0016] [Figure 7] Figure 7 is a series of graphs showing the cell viability of the liver cancer cell line Hep3B after treatment with the compound of Example 1. [Figure 8]Figure 8 is a series of graphs showing the cell viability of the liver cancer cell line HepG2 after treatment with the compound from Example 1. [Figure 9] Figure 9 is a series of photographs showing the results of treating the liver cancer cell line Hep3B with the compound from Example 1.

[0017] [Figure 10] Figure 10 is a series of photographs showing the results of treating the liver cancer cell line HepG2 with the compound from Example 1. [Figure 11] Figure 11 is a series of graphs showing the results of confirming the damaging effects of the compound of Example 1 of the present invention on various cancer cell lines. [Figure 12] Figure 12 is a graph showing the change in tumor size in a mouse model of three-type negative breast cancer allogeneic transplantation, according to the treatment method.

[0018] [Figure 13] Figure 13 is a series of photographs showing the lungs in a three-type negative breast cancer allogeneic transplant mouse model, and a graph showing the number of tumor nodules, according to the treatment method. [Figure 14] Figure 14 is a series of graphs showing the results of observing changes in immune cells in tumor cells according to treatment methods, as measured by flow cytometry. [Figure 15] Figure 15 is a series of graphs showing the changes in immune cells in the tumor cells of Figure 14 according to the treatment method, as observed through flow cytometry.

[0019] [Figure 16] Figure 16 is a series of photographs showing the results of observing changes in protein expression and immune cells in tumor cells through immunocytochemical assays. [Figure 17] Figure 17 is a series of graphs showing changes in protein expression and immune cells in tumor cells from Figure 16 through an immunocytochemical assay. [Figure 18] Figure 18 is a series of graphs showing the cell viability of tumor cells (SKOV-3 and OVCAR-3) when the compound from Example 1 and cisplatin were administered individually and in combination. [Figure 19] Figure 19 is a series of graphs showing the cell viability of tumor cells (SK Hep1 and Huh-7) when the compound from Example 1 and sorafenib were administered alone and in combination. [Modes for carrying out the invention]

[0020] Description of preferred embodiments The present invention is described in detail below. Aspects of the present invention can be modified in various other forms, and the scope of the invention is not limited to the aspects described below. It will be readily apparent to a person in the art with average skill in the art that aspects of the present invention are provided for a more precise interpretation of the invention.

[0021] In addition, the “inclusion” of elements throughout this specification does not exclude other elements, and may include other elements, unless specifically defined otherwise.

[0022] In aspects of the present invention, the present invention provides a pharmaceutical composition for preventing or treating cancer, comprising as an active ingredient a compound represented by the following formula 1, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof. [Formula 1] [ka]

[0023] In another aspect of the present invention, the compound represented by Formula 1 above may be (trans)-2,6-dichloro-4-(4-(4-hydroxycyclohexylamino)-7H-pyrrolo[2,3-D]pyrimidine-5-yl)phenol. In another aspect of the present invention, the pharmaceutical composition may be used in combination therapy with radiation or anticancer agents.

[0024] The anticancer drug may be at least one selected from the group consisting of cisplatin, sorafenib, OKN-007, gefitinib, doxorubicin, vinblastine, taxol, etoposide, 5-FU, and ifosfamide.

[0025] In another aspect of the present invention, the pharmaceutical composition may enhance immunity. In Experimental Example 9 below, changes in immune cells in the tumor microenvironment were evaluated. As a result, it was confirmed that when the compound of Example 1 was administered alone or in combination with radiation, the proportion of CD8-positive T cells increased, M1 TAM also increased, and M2 TAM decreased.

[0026] In this invention, the term "pharmaceutically acceptable salt" means salts commonly used in the pharmaceutical industry, such as inorganic ionic salts made from calcium, potassium, sodium, and magnesium; inorganic salts made from hydrochloric acid, nitric acid, phosphoric acid, bromate, iodic acid, perchloric acid, and sulfuric acid; acetic acid, trifluoroacetic acid, citric acid, maleic acid, succinic acid, oxalic acid, benzoic acid, tartaric acid, fumaric acid, mandelic acid, propionic acid, lactic acid, glycolic acid, gluconic acid, galacturonic acid, glutamic acid, gluta This refers to organic salts made from lic acid, glucuronic acid, aspartic acid, ascorbic acid, carbonic acid, vanillic acid, hydroiodic acid, etc.; sulfonates made from methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, and naphthalenesulfonic acid; amino acid salts made from glycine, arginine, lysine, etc.; and amine salts made from trimethylamine, triethylamine, ammonia, pyridine, picoline, etc., but these salts do not limit the type of salt of the present invention.

[0027] In this invention, the term "isomer" refers to a compound or salt thereof of the present invention that has the same chemical or molecular formula but is structurally or stereochemically different. Isomers include structural isomers such as tautomers and stereoisomers, and stereoisomers include both R-isomers or S-isomers (optical isomers, enantiomers) with chiral carbon centers and geometric isomers (trans, cis). In this invention, all stereoisomers of the compound represented by formula 1 and mixtures thereof are also included in the scope of this invention.

[0028] In the present invention, the term "hydrate" refers to the compound represented by Formula 1 above and water bonded together by non-covalent intermolecular forces, and may include stoichiometric or non-stoichiometric amounts of water. Specifically, the hydrate may contain water in a ratio of about 0.25 moles to about 10 moles based on 1 mole of the active ingredient, and more specifically, it may contain about 0.5 moles, about 1 mole, about 1.5 moles, about 2 moles, about 2.5 moles, about 3 moles, about 5 moles, and so on.

[0029] In the present invention, the term "solvate" refers to a solvent other than water, bonded by non-covalent intermolecular forces to the compound represented by Formula 1 above, and may include stoichiometric or non-stoichiometric amounts of water. Preferred solvents are volatile, non-harmful, and can be administered to humans in trace amounts. Specifically, the solvate may contain water in a ratio of about 0.25 moles to about 10 moles based on 1 mole of the active ingredient, and more specifically, it may contain about 0.5 moles, about 1 mole, about 1.5 moles, about 2 moles, about 2.5 moles, about 3 moles, about 5 moles, and so on.

[0030] In this invention, the term "approximately" refers to a numerical value that is ±10% of a preceding value.

[0031] In the present invention, the term "contained as an active ingredient" means contained within a dosage range that produces an effect of preventing, curing, or treating cancer, the dosage range may vary depending on the severity and formulation, and the number of applications may also vary depending on the age, weight, and physique of the subject. In one embodiment of the present invention, the compound represented by formula 1 is contained in the pharmaceutical composition of the present invention in an amount of, for example, 0.001 mg / kg or more, preferably 0.1 mg / kg or more, more preferably 10 mg / kg or more, more preferably 100 mg / kg or more, more preferably 250 mg / kg or more, and most preferably 0.1 g / kg or more. The quantitative upper limit of the compound represented by formula 1 contained in the pharmaceutical composition of the present invention may be selected within an appropriate range by those skilled in the art.

[0032] A pharmaceutical composition according to the present invention may contain an effective amount of the compound represented by Formula 1 alone, or it may also include one or more pharmaceutically acceptable carriers, excipients, or diluents.

[0033] A pharmaceutically acceptable carrier, excipient, or diluent is a physiologically acceptable substance that, when administered to humans, does not typically cause gastrointestinal upset, dizziness, or other allergic reactions, or similar reactions. Examples of carriers, excipients, and diluents include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, arginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate, and mineral oil. In addition, fillers, anticoagulants, lubricants, wetting agents, fragrances, emulsifiers, and preservatives may also be included.

[0034] The pharmaceutical compositions of the present invention may be formulated using methods known in the art to provide rapid, sustained, or delayed release of the active ingredient after administration to a subject. The pharmaceutical compositions may be formulated in the form of oral preparations, injectable preparations, or topical preparations. Oral preparations may be selected from the group consisting of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, and lyophilized preparations, but are not limited thereto. In addition, topical preparations may be selected from the group consisting of creams, gels, ointments, emulsions, suspensions, sprays, and transdermal patches, but are not limited thereto.

[0035] The pharmaceutical composition of the present invention can be administered through various routes, including oral administration, transdermal administration, subcutaneous administration, intravenous administration, or intramuscular administration. In the present invention, the term "recovery" refers to the alleviation, prevention, or treatment of cancer symptoms by administering, ingesting, or applying the pharmaceutical composition or food composition of the present invention to a person suffering from cancer.

[0036] In the present invention, the term "prevention" refers to inhibiting or blocking cancer symptoms by administering, ingesting, or applying the pharmaceutical composition or food composition of the present invention to a subject who does not have cancer. In the present invention, the term "treatment" refers to complete cure, partial cure, improvement, and alleviation of cancer symptoms as a result of administering the pharmaceutical composition of the present invention to a subject suffering from cancer.

[0037] In this invention, the term "subject" refers to any animal, including humans, that has already developed cancer or is at risk of developing cancer. The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount.

[0038] In this invention, the term "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit-to-risk ratio applicable to a medical treatment or improvement. The effective dose level depends on factors including the type and severity of the subject, age, sex, drug activity, sensitivity to the drug, time of administration, route of administration and elimination rate, duration of treatment, concomitant drugs, and other factors well known in the medical field.

[0039] In this invention, the term "administration" means providing a substance to a subject or patient by any appropriate method. The substance may be administered parenterally (e.g., intravenous, subcutaneous, intraperitoneal, or local injection) or orally according to a desired method. The dosage range will vary depending on the patient's weight, age, sex, health status, diet, administration time, method of administration, elimination rate, and severity of the disease. Specifically, in this invention, the term "parenteral administration" refers to methods of administration using a tube, subcutaneously, intramuscularly, intravenously, or intraperitoneally, but excludes oral administration. In addition, in this invention, the term "oral administration" refers to methods of administering an injectable agent orally to alleviate pathological symptoms.

[0040] For parenteral administration formulations, topical preparations such as sterile suspensions, liquids, water-insoluble excipients, suspensions, emulsions, eye drops, eye ointments, syrups, suppositories, aerosols, and sterile injection solutions can be prepared by conventional methods. Preferably, pharmaceutical compositions such as creams, gels, patches, sprays, ointments, plasters, lotions, topical medications, eye ointments, eye drops, pastes, or pap can be prepared, but are not limited to these. Water-insoluble excipients and suspensions may contain, in addition to the active compound (one or more), propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injection esters such as ethylolate, etc. Suppositories may contain, in addition to the active compound (one or more), witepsol, macrogol, tween 61, cocoa butter, lauric butter, glycerol gelatin, etc.

[0041] In the present invention, cancer may be any one selected from the group consisting of lung cancer, non-small cell lung cancer (NSCL), bronchial alveolar cell lung cancer, ovarian cancer, colorectal cancer, melanoma, gastric cancer, gastrointestinal cancer, liver cancer, bone cancer, pancreatic cancer, skin cancer, head and neck cancer, melanoma of the skin or eyeball, uterine cancer, rectal cancer, colon cancer, breast cancer, uterine sarcoma, fallopian tube cancer, endometrial cancer, cervical cancer, vaginal cancer, vulvar cancer, esophageal cancer, laryngeal cancer, small intestine cancer, thyroid cancer, parathyroid cancer, soft tissue sarcoma, urethral cancer, penile cancer, prostate cancer, multiple myeloma, and chronic or acute leukemia, and preferably, cancer may be liver cancer, lung cancer, ovarian cancer, colorectal cancer, melanoma, or breast cancer.

[0042] In another aspect of the present invention, the present invention provides a health functional food composition for preventing or treating cancer, comprising as an active ingredient a compound represented by the following formula 1, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof. [Formula 1] [ka]

[0043] Food compositions according to the present invention can be formulated in the same manner as pharmaceutical compositions and can be used as functional foods or added to various foods. Examples of foods to which the food compositions of the present invention may be added include beverages, alcoholic beverages, confectionery, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gum, ice cream, and hangover relievers (drinks, low-viscosity gels, pills, tablets, capsules, etc.), vitamin complexes, health supplements, etc.

[0044] The food composition of the present invention contains a compound represented by Formula 1, a pharmaceutically acceptable salt thereof, a hydrate thereof, or a solvate thereof as an active ingredient, and may also include ingredients commonly added during food production, such as proteins, carbohydrates, fats, nutrients, seasonings, and flavorings. Examples of carbohydrates mentioned above include monosaccharides, such as glucose, fructose, etc.; disaccharides, such as maltose, sucrose, oligosaccharides, etc.; and common sugars including polysaccharides, such as dextrin and cyclodextrin, and sugar alcohols, such as xylitol, sorbitol, and erythritol. Natural sweeteners (thaumatin, stevia extract, such as rebaudioside A, glycyrrhizin, etc.) and synthetic sweeteners (saccharin, aspartame, etc.) may be included as sweeteners. When the food composition of the present invention is formulated as a beverage or drink, it may also include citric acid, high-fructose corn syrup, sugars, glucose, acetic acid, malic acid, fruit juice, and various plant extracts in addition to the compound represented by formula 1 or 2, its pharmaceutically acceptable salt, its hydrate, or its solvate.

[0045] The present invention provides a health functional food or health supplement food containing a food composition comprising a compound represented by Formula 1, a pharmaceutically acceptable salt thereof, its hydrate, or its solvate as an active ingredient. In the present invention, the term "health functional food or health supplement food" refers to a food manufactured and processed using raw materials or ingredients that have functional properties useful to the human body in accordance with the Act on Health Functional Food. The term "functional food" refers to a food consumed for the purpose of obtaining useful health effects (such as regulating nutrients for the structure and function of the human body or physiological effects). In the present invention, a functional food is a food product prepared by adding a compound represented by Formula 1, a pharmaceutically acceptable salt thereof, its hydrate, or its solvate to food materials such as beverages, teas, spices, gums, and confectionery, or by encapsulation, powdering, or suspension. The functional foods described above have specific health benefits when consumed, but because they are manufactured using food as a raw material, they have the advantage of not having any of the side effects that can occur when drugs are taken over a long period of time, unlike general drugs. The functional foods or health supplements of the present invention obtained in this way are extremely useful as they can be taken on a daily basis. The amount of the compound represented by Formula 1, its pharmaceutically acceptable salt, its hydrate, or its solvate in such functional foods or health supplements depends on the type of functional food being targeted, but is added within a range that does not impair the original taste of the food, and is generally in the range of 0.01 to 50 wt%, preferably 0.1 to 20 wt%, of the target food, so it cannot be prescribed uniformly. In addition, in the case of functional foods or health supplements in the form of pills, granules, tablets, or capsules, it is usually added in the range of 0.1 to 100 wt%, preferably 0.5 to 80 wt%. In one embodiment of the present invention, the health functional food or health supplement of the present invention may be in the form of pills, tablets, capsules, or beverages.

[0046] The food composition of the present invention may contain conventional food additives. Unless otherwise specified, the suitability as a "food additive" is determined according to the general rules and general test methods for food additives approved by the Ministry of Food and Drug Safety, and according to the standards and criteria applicable to the item in question.

[0047] The items listed in the "Korean Food Additives Code" include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid; natural additives such as persimmon color, licorice extract, crystalline cellulose, sorghum color, and guar gum; and mixed preparations such as L-sodium glutamate preparations, alkaline agents for noodles, preservative preparations, and tar dye preparations.

[0048] In addition, the food composition of the present invention may be prepared and processed into the form of tablets, capsules, powders, granules, liquids, pills, etc., for the purpose of preventing and / or curing cancer.

[0049] For example, a functional food in tablet form may be prepared by conventionally granulating a mixture of a food composition containing a compound represented by Formula 1, a pharmaceutically acceptable salt thereof, its hydrate, or its solvate as an active ingredient, and excipients, binders, disintegrants, and other additives, and then compressing the mixture with a lubricant or the like, or by directly compressing the mixture. In addition, functional foods in tablet form may optionally contain a flavor enhancer or the like, and may optionally be coated with a suitable coating agent.

[0050] Among health functional foods in capsule form, hard capsules can be prepared by filling conventional hard capsules with a mixture of a food composition containing a compound represented by Formula 1, its pharmaceutically acceptable salt, its hydrate, or its solvate as an active ingredient, and additives such as excipients, or granularity thereof, or coated granularity thereof. Soft capsules can be prepared by filling a mixture of a food composition containing a compound represented by Formula 1, its pharmaceutically acceptable salt, its hydrate, or its solvate as an active ingredient, and additives such as excipients, into a capsule base such as gelatin. Soft capsules may contain plasticizers such as glycerin or sorbitol, colorants, preservatives, etc., if necessary.

[0051] Health functional foods in pill form can be prepared by forming a mixture of a food composition containing a compound represented by Formula 1, a pharmaceutically acceptable salt thereof, its hydrate, or its solvate as an active ingredient, with excipients, binders, disintegrants, etc., in any preferred manner, and if necessary, they may be coated with white sugar or another preferred coating agent, or with starch, talc, or other preferred substances.

[0052] Health functional foods in granular form can be prepared by using a mixture of a food composition containing a compound represented by Formula 1, a pharmaceutically acceptable salt thereof, its hydrate, or its solvate as an active ingredient, and excipients, binders, disintegrants, etc., in any preferred manner, and may contain flavoring agents, flavor enhancers, etc., if necessary. When particle size tests were performed using No. 12 (1680 μm), No. 14 (1410 μm), and No. 45 (350 μm) sieves, the entire amount of the health functional food in granular form passed through the No. 12 sieve, less than 5.0% of the total amount remained in the No. 14 sieve, and less than 15.0% of the total amount passed through the No. 45 sieve.

[0053] There are no specific limitations on the type of food; it encompasses all health functional foods in the conventional sense. The matters referred to in the pharmaceutical and food compositions of the present invention shall apply in the same manner, provided they do not contradict each other.

[0054] In another aspect of the present invention, the present invention provides a pharmaceutical kit for preventing or treating cancer, comprising: a first component containing a pharmaceutically effective amount of an anticancer agent; and a second component containing as an active ingredient a compound represented by the following formula 1, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof. [Formula 1] [ka]

[0055] In another aspect of the present invention, the compound represented by Formula 1 above may be (trans)-2,6-dichloro-4-(4-(4-hydroxycyclohexylamino)-7H-pyrrolo[2,3-D]pyrimidine-5-yl)phenol.

[0056] In another aspect of the present invention, cancer may be any one selected from the group consisting of lung cancer, non-small cell lung cancer (NSCL), bronchioloalveolar cell lung cancer, ovarian cancer, colorectal cancer, melanoma, gastric cancer, gastrointestinal cancer, liver cancer, bone cancer, pancreatic cancer, skin cancer, head and neck cancer, melanoma of the skin or eye, uterine cancer, rectal cancer, colon cancer, breast cancer, uterine sarcoma, fallopian tube cancer, endometrial cancer, cervical cancer, vaginal cancer, vulvar cancer, esophageal cancer, laryngeal cancer, small intestine cancer, thyroid cancer, parathyroid cancer, soft tissue sarcoma, urethral cancer, penile cancer, prostate cancer, multiple myeloma, and chronic or acute leukemia.

[0057] In another aspect of the present invention, the anticancer agent may be at least one selected from the group consisting of cisplatin, sorafenib, OKN-007, gefitinib, doxorubicin, vinblastine, taxol, etoposide, 5-FU, and ifosfamide.

[0058] In another aspect of the present invention, the present invention provides a method for preventing or treating cancer, comprising the step of administering a compound represented by Formula 1 as described herein, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof, to a subject in need thereof.

[0059] In another aspect of the present invention, the present invention provides a method for preventing or treating cancer, comprising the step of co-administering a compound represented by Formula 1 as described herein, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof, with radiation or an anticancer agent to a subject in need thereof.

[0060] In another aspect of the present invention, the present invention provides the use of a compound represented by Formula 1 as described herein, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof, for the preparation of a pharmacopoeia for use in the prevention or treatment of cancer.

[0061] In another aspect of the present invention, the present invention provides the use of a compound represented by Formula 1 as described herein, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof, in combination with a radioactive or anticancer agent for the preparation of a pharmacopoeia for use in the prevention or treatment of cancer. The above methods or uses may be applied to the detailed descriptions of the pharmaceutical compositions described above.

[0062] In the following text, the present invention will be described in detail by the following examples and experimental examples. However, the following examples and experimental examples are for illustrative purposes only, and the scope of the present invention is not limited thereto.

[0063] Example 1: Preparation of (trans)-2,6-dichloro-4-(4-(4-hydroxycyclohexylamino)-7H-pyrrolo[2,3-d]pyrimidine-5-yl)phenol [Reaction Equation 1] [ka]

[0064] Step 1 DMF (30 mL) was added to 4-chloro-7H-pyrrolo[2,3-d]pyrimidine (2 g, 13.02 mmol), and NBS (2.52 g, 14.24 mmol) was added at 0°C. The mixture was stirred at room temperature for 3 hours. After the reaction was complete, the solvent was removed under high pressure, the resulting mixture was mixed with water and filtered, washed with hexane and dried to give 5-bromo-4-chloro-7H-pyrrolo[2,3-d]pyrimidine (2.66 g, 88%). 1 H NMR (DMSO, 300MHz): δ7.81(s,1H),8.76(s,1H),5.02(br s,1H).

[0065] Step 2 NaH (0.41 g, 10.3 mmol) was added to 5-bromo-4-chloro-7H-pyrrolo[2,3-d]pyrimidine (1.2 g, 5.16 mmol) dissolved in DMF (10 mL), and the mixture was stirred at 0°C for 30 minutes. After adding p-tosyl chloride (1.36 g, 7.16 mmol), the mixture was stirred at room temperature for 6 hours. Once the reaction was complete, water was added and the mixture was stirred for 10 minutes. The resulting product was collected by filtration and dried to give 5-bromo-4-chloro-7-tosyl-7H-pyrrolo[2,3-d]pyrimidine (1.79 g, 90%). 1 H NMR (CDCl3,300MHz): δ8.76(s,1H),8.09(d,2H,J=8.1Hz),7.54(s,1H),7.34(d,2H,J=8.1Hz),2.41(s,3H).

[0066] Step 3 N-BuOH (10 mL) was added to 5-bromo-4-chloro-7-tosyl-7H-pyrrolo[2,3-d]pyrimidine (500 mg, 1.29 mmol), to which trans-4-aminocyclohexane-1-ol (223 mg, 1.94 mmol) and DIPEA (2.58 mmol) were added. The mixture was heated at 110°C for 3 hours. The solvent was removed under high pressure, and the residue was purified by chromatography to obtain (trans)-4-((5-bromo-7-tosyl-7H-pyrrolo[2,3-d]pyrimidine-4-yl)amino)cyclohexane-1-ol (540 mg, 90%). 1 H NMR(CDCl3,300MHz):δ8.37(s,1H),8.06(d,2H,J=8.3Hz),7.45(s,1H),7.31(d,2H,J=8.2Hz),5.87 (d,1H),4.12(m,1H),3.70(m,1H),2.40(s,3H),2.16(m,2H),2.03(m,2H),1.54(m,2H),1.31(m,2H).

[0067] Step 4 (Trans)-4-((5-bromo-7-tosyl-7H-pyrrolo[2,3-d]pyrimidine-4-yl)amino)cyclohexane-1-ol (200 mg, 0.43 mmol), 2,6-dichloro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenol (0.86 mmol), Na2CO3 (0.86 mmol), dioxane (4 mL), and water (1 mL) were placed in a microwave vial. The solvent was degassed for 15 minutes, and Pd(PPh3)2Cl2 (10 mol%) was added. The vial was then irradiated with microwaves at 80°C for 30 minutes. The solution was filtered through a Celite layer, and the filtrate was washed with brine (10 mL x 5). The organic layer was concentrated and subjected to chromatography (10% methanol: dichloromethane) to obtain (trans)-2,6-dichloro-4-(4-((-4-hydroxycyclohexyl)amino)-7-tosyl-7H-pyrrolo[2,3-d]pyrimidine-5-yl)phenol (40%). 1H NMR(CDCl3,300MHz):δ8.46(s,1H),8.11(d,2H,J=8.36Hz),7.41(s,1H),7.36(s,2H),7.31(d,2H,J=8.13Hz),6.0 3(m,1H),4.71(d,1H),4.09(m,1H),3.63(m,1H),2.41(s,3H),2.05(m,2H),1.89(m,2H),1.40(m,2H),1.10(m,2H).

[0068] Step 5 1M TBAF (in THF) was added to the above intermediate (trans)-2,6-dichloro-4-(4-((-4-hydroxycyclohexyl)amino)-7-tosyl-7H-pyrrolo[2,3-d]pyrimidine-5-yl)phenol and stirred at room temperature for 20 hours. The reaction product was purified under reduced pressure by chromatography (15% methanol:dichloromethane + 0.1% aqueous ammonia) to give the title compound (40%). 1 H NMR(DMSO-d6,300MHz):δ11.82(br s,1H),10.16(br s,1H),8.17(s,1H),7.42(s,2H),7.29(s,1H),5.27(d,1H),4.54(m,1H),4. 01(m,1H),3.43(m,1H),1.95(m,2H),1.76(m,2H),1.40(m,2H),1.20(m,4H).

[0069] Experimental Example 1: Evaluation of Inhibition of Major Protein Expression Experiments were conducted using Western blotting to confirm the inhibitory effect of the compound in Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, on the expression of major proteins during cancer cell growth.

[0070] Specifically, MDA-MB-231 cells, a TNBC cell line, were cultured at 37°C in a 5% CO2 incubator, and the culture medium used was RPMI-1640 supplemented with 10% FBS. 5 x 10¹⁴ BALB / c nude mice (5 mice each in the control and treatment groups) were subcutaneously implanted in the right neck. 6Tumors were induced by injecting 100 μl of MDA-MB-231 cells, resulting in a tumor size of 200 mm. 3 The experiment was conducted when the target was reached. The compound of Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, was administered once by intravenous injection at concentrations of 5, 10, and 20 mg / kg (T4, T5, and T6), followed by euthanasia using CO2. Tumor tissue obtained from the control group and the treatment group was collected and subjected to Western blotting. Proteins were isolated from the tumor tissue, and the results of Western blotting were confirmed using antibodies against BRD4, c-myc, PD-L1, and actin, respectively.

[0071] When the expression of BRD4, c-myc, and PD-L1 in T4, 5, and 6 groups treated with the compound of Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, was compared with that of an untreated negative control (NC), it was confirmed that the expression of proteins important for oncogenesis was reduced in T4, 5, and 6 groups.

[0072] Experimental Example 2: Evaluation of inhibition of breast cancer cell lines Experiments were conducted to confirm the growth inhibitory effect of the compound in Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, on breast cancer cell lines through a cell viability assay.

[0073] Specifically, the following cell viability assays were performed: Human-derived breast cancer cell line BT20 and TNBC cell line MDA-MB-231 were each placed in 96-well plates in 5 × 10⁶ cells. 3 Cells were seeded at a cell / well density and cultured for 24 hours. Then, I-bet 762 and the compounds from Example 1 were treated at various concentrations and cultured for 48 hours. At the end of the culture, 100 μl of WST-8 solution was added to each well of the plate, and absorbance was measured using a 450 nm optical filter with a microplate reader to determine the formazan produced by reducing factors in the living cells.

[0074] As shown in Figures 2 and 3, analysis of tumor-like mass formation by culturing human-derived breast cancer cell line BT20 and TNBC cell line MDA-MB-231 confirmed that the compound of Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, suppressed cell viability in a dose-dependent manner not only in common breast cancer cell lines but also in TNBC cell lines.

[0075] Figure 4 shows the cell viability of MDA-MB-231, MDA-MB-453, and BT-20 24 hours after treatment with the compound from Example 1. As shown in Figure 4, we confirmed that cell viability was suppressed in a dose-dependent manner not only in common breast cancer cell lines but also in TNBC cell lines.

[0076] Experimental Example 3: Evaluation of Breast Cancer Growth Inhibition Experiments were conducted to confirm the tumor inhibition of the compound in Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, against xenograft tumor samples of breast cancer cells (MBA-MB-231), a human-derived TNBC cell line.

[0077] Specifically, experiments were conducted using 80 female 5-week-old xenotransplanted Balb / c nude mice to confirm the in vitro inhibitory effect of the compound in Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, on the survival rate of breast cancer cells. The mice were approximately 8 weeks old at the start of administration.

[0078] Experimental xenotransplant Balb / c nude mice lack T cells, which are immune cells, and were selected as a suitable animal model for transplanting human cancer cells. Animals were selected that were within ±20% of the total mean body weight at the time of administration, and tumor growth was checked from the surface twice a week, with tumor size measured with calipers for three weeks.

[0079] As shown in Figure 5, it was confirmed that the compound in Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, was able to significantly inhibit tumor growth.

[0080] Experimental Example 4: Comparison of Apoptotic Body Frequencies Experiments were conducted to confirm the tumor necrotic effect of the compound in Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, by measuring the number of apoptotic bodies in tumors and the H&E staining of tumor tissue in BALB / c-nude mice injected with cancer cell lines.

[0081] Specifically, to measure the number of apoptotic bodies in the tumor tissue of the T4, T5, and T6 groups in Experimental Example 1, five areas where tumor tissue was growing were randomly selected, and the number of apoptotic bodies was measured at x400 magnification. The sum of the measured values ​​was calculated and expressed as individual values, and statistically compared using GraphPad Prism 5.

[0082] Observation of apoptotic bodies revealed that the cells had shrunk and divided (forming blebs), and that cavities (hollows) surrounded them. In addition, increased cytoplasmic eosinophilicity was observed, the nuclei were smaller, concentrated or fragmented, and phagocytosed by surrounding cells.

[0083] According to the number of apoptotic bodies measured in Table 1, the number was dose-dependently higher in the T4, T5, and T6 groups treated with the compound of Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, compared to the NC (negative control). This confirms that the compound of Example 1 of the present invention has a tumor-suppressing effect by increasing apoptosis in cancer cells.

[0084] As shown in Figure 6, the central area was excised, a paraffin block was prepared through a general tissue processing process, and the tissue was thinned to a thickness of 3 μm using a microtome. The sections were then stained with hematoxylin and eosin, and the areas of cell necrosis were observed.

[0085] When comparing the stained tissues of the NC group with those of the T4, T5, and T6 groups treated with the compound of Example 1, the area of tissue necrosis in the group treated with the compound of Example 1 was larger. Thus, it was confirmed that the compound of Example 1 causes necrosis of tumor tissue and has a tumor-suppressing effect.

[0086]

Table 1

[0087] Experimental Example 5: Evaluation of Inhibition of Liver Cancer Cell Lines It was confirmed whether the compound of Example 1, which is the active ingredient of the pharmaceutical composition of the present invention, also has a growth inhibitory effect on cancer cell lines other than TNBC.

[0088] Specifically, the WST-8 assay for checking the cell viability of HepG2 and Hep3B, which are hepatocellular carcinoma cell lines shown in FIGS. 7 and 8, was performed as follows. HepG2 and Hep3B were each seeded in 96-well plates at a density of 5×10 3 cells / well and cultured for 24 hours. Next, sorafenib and OPT-0139 were serially diluted at 2-fold concentrations and then treated at various concentrations to the plates, followed by culturing for 24, 48, and 72 hours. 100 μl of WST-8 solution was added to each well of the plate, and the absorbance was measured using a microplate reader with a 450 nm optical filter to measure the formazan produced by the reducing factors in living cells.

[0089] Cells not treated with sorafenib and the compound of Example 1 showed a dark orange color, while cells treated with sorafenib and the compound of Example 1 showed a light pink color, which is the color of the culture medium of the cell line. Therefore, the number of living cells can be measured by measuring the absorbance.

[0090] As shown in FIGS. 9 and 10, the colony formation assay was performed as follows. HepG2 and Hep3B were each seeded in 12-well plates at 3×10 3Cells were seeded at a cell / well density and cultured for 24 hours. Then, sorafenib and the compound from Example 1 were treated with 2.5 μM and 5 μM, respectively, and cultured for 10 days. After culturing was complete, the cells were washed with PBS, fixed with 37% formaldehyde, and stained with 0.01% crystal violet. After staining, the cells were washed with distilled water, and the colonies of stained cells were photographed.

[0091] Observation of stained cell colonies revealed a clear difference in the number of colonies, indicating cell viability, between areas treated with sorafenib and the compound from Example 1 and areas treated with the compound.

[0092] As shown in Figures 7-10, cell viability was confirmed using WST-8 in liver cancer cell lines Hep3B and HepG2. The results showed that treatment with the compound in Example 1 and sorafenib reduced cell viability in a dose-dependent and time-dependent manner, demonstrating a remarkable effect compared to sorafenib, an existing targeted anticancer agent.

[0093] Experimental Example 6: Evaluation of cytotoxicity against various cancer cell lines The cytotoxicity of the compound in Example 1 against various cancer cell lines was evaluated through a process similar to that used to evaluate the inhibition of liver cancer cell lines described above. Etoposide, which is marketed as an anticancer drug, was used as a control drug.

[0094] The results are shown in Figure 11. Figure 11 is a series of graphs showing the results of confirming the damaging properties of the compound of Example 1 of the present invention against various cancer cell lines.

[0095] As shown in Figure 11, the compound of Example 1 according to the present invention showed excellent dose-dependent toxicity against cancer cell lines A549, SK-OV-3, SK-MEL-2, and HCT15. Therefore, it can be effectively used as an active ingredient in anticancer drugs.

[0096] Experimental Example 7: Evaluation of tumor size changes according to treatment methods in a three-type negative breast cancer allograft mouse model. Triple-negative breast cancer cells from 4T1 mice (6x10)5 The cells were injected into the hind limbs of immune, 6-week-old BALB / c mice. After confirming steady tumor growth 7 days post-injection, 10 mice were selected from each group and used for subsequent experiments.

[0097] The experimental groups consisted of a control group (no treatment), a radiotherapy group, a group treated with the compound from Example 1, and a group treated with a combination of the compound from Example 1 and radiotherapy. Each treatment was performed 31 days after tumor transplantation.

[0098] Radiotherapy was performed using an electron beam, with a total of 24 Gy delivered in three doses every two days for one week (days 10, 12, and 14) (8 Gy x 3). The compound from Example 1 was administered intravenously at a dose of 10 mg / kg every two or three days for a total of six doses over two weeks (days 10, 12, 14, 17, 19, and 21). On days when radiotherapy and drug administration were performed simultaneously, the compound from Example 1 was administered four hours after radiotherapy, after confirming that the mice had recovered from anesthesia.

[0099] Use calipers to measure the length and width of the tumor on each treatment day, and then use Formula 1 below to measure the tumor volume (mm²). 3 ) was calculated. [Formula 1] Volume (mm 3 ) = Length (mm) x Width (mm) 2 x 0.5

[0100] In the same manner as above, tumors were injected into 10 mice for each treatment method, and the tumor growth was observed for 31 days. The results are shown in Figure 12. As a result, it was confirmed that administration of the compound in Example 1 alone significantly delayed tumor growth compared to the control group. In addition, it was also confirmed that the combination administration of the compound in Example 1 with radiation had a superior inhibitory effect on tumor growth compared to each administration alone.

[0101] Experimental Example 8: Evaluation of lung metastases according to treatment methods in a three-type negative breast cancer allograft mouse model. Mice were prepared using the same method as in Experimental Example 7, and the lungs of the mice were removed 31 days after tumor injection. The results of observing lung metastases are shown in Figure 13. As a result, it was confirmed that administration of the compound in Example 1 alone significantly reduced metastasis compared to the control group. In addition, it was also confirmed that the combination administration of the compound in Example 1 with radiation significantly reduced lung metastasis (P<0.05) compared to each compound administered alone.

[0102] Experimental Example 9: Evaluation of immune cell changes in the tumor microenvironment according to treatment methods in a three-type negative breast cancer allogeneic transplant mouse model. Tumor tissue from each mouse was isolated as single cells, followed by flow cytometry analysis (FACS) to confirm the immunomodulatory effects.

[0103] Specifically, 31 days after tumor cell injection, the spleen and tumors of mice were extracted using the same method as in Experimental Example 7. After excision of the tumor tissue, it was mechanically processed by finely chopping it using a sterile razor blade, and then treated with DNase (0.1 mg / ml) and collagenase (1 mg / ml), respectively, and reacted at 36°C for 30 minutes to obtain a single-cell suspension. After isolating single cells from the tumor tissue, 1 × 10 cells per FACS tube were stained with FITC, PE, PerCP / Cy5.5, and APC fluorescence using CD3, CD8b, CD4, CD11b, F4 / 80, and CD206 antibodies to analyze invasive leukocytes. For each stained sample, the CD8+ cytotoxic T cell fraction was calculated using the BD Bioscience FACSCalibur instrument. FACS results were analyzed using FlowJo software (version 10).

[0104] Figures 14 and 15 show the observed changes in immune cells in the tumor microenvironment following the treatment methods described above.

[0105] CD8+ T cells are known to be cytotoxic T cells capable of destroying tumor cells. The results of the above experiment confirmed that the proportion of CD8+ T cells increased when the compound in Example 1 was administered alone. In addition, when the compound in Example 1 was administered in combination with radiation, the proportion of CD8+ T cells also increased significantly compared to each administration alone.

[0106] When the compound in Example 1 was administered alone, the M1 TAM, which has an antitumor effect, increased significantly (P<0.01), and the M2 TAM, which has an immunosuppressive effect, decreased significantly (P<0.01). In addition, when the compound in Example 1 was administered in combination with radiation, an increase in M1 TAM and a decrease in M2 TAM were also observed.

[0107] Experimental Example 10: Evaluation of changes in protein expression in the tumor microenvironment according to treatment methods in a three-type negative breast cancer allogeneic transplant mouse model. Bromodomain and extraterminal domain (BET) family proteins have recently become important targets for cancer treatment, and BRD4, in particular, a member of the BET family, is commonly found in hematological malignancies and solid tumors. Therefore, in addition to its downregulatory effects on oncogenes, BRD4 inhibitors inhibit tumor growth by directly suppressing tumor cell proliferation. In addition, hypoxia-inducible factor 1α (HIF-1α) protein is a key factor that regulates tumor cell division, angiogenesis, invasion, and metastasis in the tumor microenvironment.

[0108] To evaluate changes in the expression of proteins such as BRD4 and HIF-1α, tumor cells isolated in Experimental Example 9 were subjected to immunocytochemistry (IHC) analysis to assess protein expression in the tumor microenvironment according to the treatment method. The results are shown in Figures 16 and 17.

[0109] Specifically, tumor tissue was extracted, fixed in 4% paraformaldehyde, and embedded in paraffin to create a block. The paraffin block containing the tissue was sliced ​​transversely into 4 μm thick sections, and slides were attached to them. The paraffin was removed from the tissue to which the slides were attached using xylene and ethanol, and endogenous peroxidase was removed from the tissue by immersing the slides in a 3% H2O2 solution in methanol at room temperature for 10 minutes. Next, for antigen retrieval, the slides were boiled in 0.01 M sodium citrate buffer (pH 6.0). To exclude nonspecific binding, FcR on the cell surface was blocked using 5% normal goat serum. Next, the slides were incubated overnight at 4°C with primary antibodies (HIF-1α (SantaCruz, sc-13515), PD-L1 (Abcam, ab2025921), CD8 (Abcam, ab203035), and CD68 (CellSignaling, 97778)). Secondary antibodies were attached, and chromogenic development was induced using the ImmPRESS Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Kit (Vector Laboratories), Abcam (ab150165, ab150081), and the REAL EnVision detection system (Dako). The stained tissues were observed at 40× magnification under an Axioskop40 optical microscope (Carl Zeiss), and images were saved using AxioVision 4.7 software. Expression levels were measured through the area of ​​the stained region in the images using Image J software (NIH, Bethesda). The average density value was calculated from at least three slides per sample.

[0110] As a result, Brd4 expression was significantly reduced (P<0.001) by treatment with the compound in Example 1, and HIF-1α expression, which is known to be associated with Brd4, was also significantly reduced (P<0.05) in tumor tissue. In addition, the compound in Example 1 was found to significantly reduce (P<0.01) the expression of PD-L1, which is expressed by cancer cells to induce immune evasion.

[0111] Consistent with the flow cytometry results of Experimental Example 9, an increase in the CD8+ T cell population was observed due to both radiation and the administration of the compound in Example 1, respectively. Furthermore, it was confirmed that the CD8+ T cell population increased even further (synergistic effect) when the compound in Example 1 was administered in combination with radiation. Additionally, the CD68+ macrophage population in tumor tissue was significantly reduced (P<0.01) by the compound in Example 1.

[0112] Experimental Example 11: Evaluation of efficacy in combination with other anticancer drugs <11-1> Evaluation of efficacy in combination with cisplatin Human ovarian cancer cell lines (SKOV-3 and OVCAR-3) were treated with a control (untreated), 0.1 μM of the compound from Example 1, 1 μM of cisplatin, and 0.1 μM of the compound from Example 1 / 1 μM of cisplatin, and the cell viability of SKOV-3 and OVCAR-3 was examined. The results are shown in Figure 18.

[0113] When 0.1 μM of the compound from Example 1 or 1 μM of cisplatin was administered to SKOV-3 and OVCAR-3 cell lines, cell viability was suppressed. In addition, it was confirmed that when 0.1 μM of the compound from Example 1 and 1 μM of cisplatin were administered in combination, cell viability was further inhibited (synergistic effect).

[0114] <11-2> Evaluation of efficacy in combination with sorafenib Human liver cancer cell lines (SK Hep1 and Huh-7) were treated with a control (untreated), 0.1 μM of the compound from Example 1, 1 μM of sorafenib, and 0.1 μM of the compound from Example 1 / 1 μM of sorafenib, and the cell viability of SK Hep1 and Huh-7 was examined. The results are shown in Figure 19.

[0115] When 0.1 μM of the compound from Example 1 or 1 μM of sorafenib was administered to SK Hep1 and Huh-7 cell lines, cell viability was suppressed. In addition, it was confirmed that when 0.1 μM of the compound from Example 1 and 1 μM of sorafenib were administered in combination, cell viability was further inhibited (synergistic effect).

[0116] From the above results, it was confirmed that the compound of Example 1 according to the present invention inhibits the formation of tumor-like masses in breast cancer cell lines and exhibits a significantly greater or equal effect than sorafenib and etoposide, which are topoisomerase inhibitors widely used in lung cancer, ovarian cancer, colon cancer, melanoma, etc. In addition, the compound of Example 1 according to the present invention exhibits a synergistic anticancer effect when combined with radiotherapy or other anticancer drugs in breast cancer cell lines and liver cancer cell lines, and can therefore be developed as an anticancer drug or food that exhibits excellent effects in the treatment of cancer.

Claims

1. Equation 1 below: [Formula 1] 【Chemistry 1】 A pharmaceutical composition for use in the prevention or treatment of cancer, comprising as an active ingredient a compound represented by, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof, The aforementioned cancer is selected from the group consisting of breast cancer, liver cancer, non-small cell lung cancer (NSCL), ovarian cancer, melanoma, and colorectal cancer, and is the pharmaceutical composition.

2. The pharmaceutical composition for use in the prevention or treatment of cancer according to claim 1, wherein the compound represented by the above formula 1 is (trans)-2,6-dichloro-4-(4-(4-hydroxycyclohexylamino)-7H-pyrrolo[2,3-D]pyrimidine-5-yl)phenol.

3. A pharmaceutical composition for use in the prevention or treatment of cancer according to claim 1, wherein the cancer is breast cancer or liver cancer.

4. A pharmaceutical composition for use in the prevention or treatment of cancer, according to claim 1, wherein the pharmaceutical composition is used for treatment in combination with radiotherapy or an anticancer agent.

5. A pharmaceutical composition for use in the prevention or treatment of cancer according to claim 4, wherein the anticancer agent is at least one selected from the group consisting of cisplatin, sorafenib, Opdivo, Tecentriq, Keytruda, Imfinzi, OKN-007 (Oklahoma nitrone-007), gefitinib, doxorubicin, vinblastine, taxol, etoposide, 5-FU (5-fluorouracil), and ifosfamide.

6. Equation 1 below: [Formula 1] 【Chemistry 2】 A health functional food composition for use in the prevention or recovery of cancer, comprising as an active ingredient a compound represented by, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof, The aforementioned cancer is selected from the group consisting of breast cancer, liver cancer, non-small cell lung cancer (NSCL), ovarian cancer, melanoma, and colorectal cancer, as described in the health functional food composition.

7. A first component containing a pharmaceutically effective amount of anticancer agent; and Equation 1 below: [Formula 1] 【Transformation 3】 A second component containing as an active ingredient a compound represented by, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof. A medical kit for preventing or treating cancer, including The cancer is selected from the group consisting of breast cancer, liver cancer, non-small cell lung cancer (NSCL), ovarian cancer, melanoma, and colorectal cancer, as described in the pharmaceutical kit.

8. A pharmaceutical kit for preventing or treating cancer according to claim 7, wherein the compound represented by formula 1 above is (trans)-2,6-dichloro-4-(4-(4-hydroxycyclohexylamino)-7H-pyrrolo[2,3-D]pyrimidine-5-yl)phenol.

9. A pharmaceutical kit for preventing or treating cancer according to claim 7, wherein the anticancer agent is at least one selected from the group consisting of cisplatin, sorafenib, Opdivo, Tecentriq, Keytruda, Imfinzi, OKN-007 (Oklahoma nitrone-007), gefitinib, doxorubicin, vinblastine, Taxol, etoposide, 5-FU (5-fluorouracil), and ifosfamide.

10. Use of a compound represented by Formula 1 as described in claim 1, its stereoisomer, its solvate, its hydrate, or a pharmaceutically acceptable salt thereof, for the preparation of a medicament for use in the prevention or treatment of cancer.

11. Use of a compound represented by Formula 1 as described in claim 1, a stereoisomer thereof, a solvate thereof, a hydrate thereof, or a pharmaceutically acceptable salt thereof, in combination with a radioactive or anticancer agent for the preparation of a medicament for use in the prevention or treatment of cancer.

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