Observation system and focusing mechanism for the observation system

Abstract

To provide an observation system that makes it possible to perform the work for adjusting an object to be observed into an observable state quickly and easily and to simply and securely perform the operation concerning the improvement of observation work efficiency and observation accuracy in the observation of the object to be observed and to provide a focus adjustment mechanism.SOLUTION: The observation system includes imaging means for acquiring image data of a subject to be observed, observation means for continuously observing by imaging means a sheet on which the subject to be observed is arranged and a plurality of imaging subjects arranged on the sheet, and a focus adjustment mechanism that is linked to the observation means and adjusts the focus of the imaging means. In continuously observing a subject to be observed arranged on the sheet by imaging means, it becomes possible to adjust the focus regardless of a positional adjustment of a light-receiving unit on the imaging means side by providing a focus adjustment mechanism that is linked to the observation means side.SELECTED DRAWING: Figure 2

Description

Technical Field

[0001] The present invention relates to an observation system and a focusing mechanism for an observation system.

Background Art

[0002] In recent years, with the improvement of information processing technology and observation technology, it has become possible to acquire and analyze a large amount of data. Along with this, the importance of creating databases by acquiring and analyzing observation data for various observation objects and effectively utilizing the data has been increasing.

[0003] Particularly, in the acquisition of observation data by microscopic observation, in addition to the acquisition of image data related to the shape of the observation object, it has also become possible to acquire measurement data and the like by combining various optical analysis means, and the amount of information that can be utilized as observation data has increased significantly.

[0004] While the technologies related to the acquisition and analysis of various observation data have been improving, sample preparation for making the observation object in a state suitable for observation still largely relies on manual work. As the amount of the observation object increases, the labor cost and time consumption related to the sample preparation of the observation object have become extremely large. Since it is necessary to quickly perform operations related to the observation of a large number of observation objects for database creation and effective utilization of data, the improvement of efficiency related to the sample preparation of the observation object has been under consideration.

[0005] For example, Patent Document 1 describes an apparatus for automatically processing an observation object (biological specimen), which includes a slide tray for holding a slide on which the observation object is placed, a conveyance device for the slide tray, a fluid module for supplying a reagent, and an automatic cover glass mounting processing device.

Prior Art Documents

Patent Documents

[0006]

Patent Document 1

[0007] As described in Patent Document 1, it is known that the preparation of so-called slides using cover slips and slides is automated in order to quickly observe multiple objects. However, the preparation of microscope slides generally involves a complex process, starting with placing the object to be observed on the slide glass, supplying reagents, placing the coverslip, and transporting the slide to the microscope stage. Therefore, while the automation of slide preparation described in Patent Document 1 can significantly reduce labor costs, challenges remain regarding the efficiency of preparing large quantities of samples and observing them, such as the need to replace the prepared sample after observation.

[0008] Furthermore, with the recent advancements in medical and biological technologies, there has been an increase in the use of cells and microorganisms as objects of observation. In this context, there is a growing demand to observe living organisms (hereinafter simply referred to as "living organisms") such as living cells and microorganisms, rather than dead cells or extinct microorganisms, and to acquire observational data from them. Conventional sample preparation methods, such as microscope slides, present a problem in that the state and position of the object being observed may change during the process of preparing the slide and moving it to the microscope stage. This makes the procedure for acquiring observation data more complicated and makes it difficult to obtain accurate observation data. Furthermore, when observing living organisms, sample preparation using multi-well plates with multiple wells is known, but in this case, it is difficult to fix the living organism, making observation difficult. For these reasons, there is a need for new observation techniques that do not rely on microscope slides or multi-well plates used in conventional sample preparation.

[0009] The inventors have already discovered an observation system that enables sample preparation and observation without the use of slides or multi-well plates. This system allows for continuous sample preparation and observation by placing the object to be observed on a sheet and moving the sheet, thereby further improving the efficiency of work related to the observation of a large number of objects. On the other hand, when observing objects placed on a sheet, as in this observation system, the system is affected by environmental fluctuations around the observation system, such as heat generated by the operation of the equipment, the on / off switching of air conditioning, and changes in room temperature, which can cause a shift in the focus position during observation with the imaging device. Generally, focusing in observation using an imaging device is known to be done by manipulating the position of the light-receiving part (lens) on the imaging device used for observation. However, in an observation system that continuously observes objects placed on a sheet, it is difficult to manually adjust the focus by manipulating the light-receiving part. Furthermore, if a so-called autofocus function is provided to the imaging device, the overall size of the observation system becomes larger. Therefore, there is a need for a simpler method for focusing, which is an operation that improves the efficiency and accuracy of observation work.

[0010] The object of the present invention is to provide an observation system and a focusing mechanism for the observation system that enable the preparation of an object for observation to be made quick and easy, and that allow for simple and reliable operation to improve observation efficiency and accuracy. [Means for solving the problem]

[0011] As a result of diligent study on the above-mentioned problems, the inventors of the present invention have found that by placing the object to be observed on a sheet and performing continuous observation using an imaging means to acquire image data, and by adjusting the focus of the imaging means in conjunction with the means related to continuous observation, the work related to the preparation of the object to be observed can be made quick and easy, and operations related to improving the efficiency and accuracy of observation can be performed simply and reliably, thereby completing the present invention. In other words, the present invention relates to the following observation system and focusing mechanism for the observation system.

[0012] The observation system of the present invention, which solves the above problems, is characterized by comprising: an imaging means for acquiring image data of an object to be observed; a sheet on which the object to be observed is placed; an observation means for continuously observing a plurality of objects to be observed placed on the sheet using the imaging means; and a focus adjustment mechanism that works in conjunction with the observation means to adjust the focus of the imaging means. The observation system of the present invention allows for sample preparation and observation without the need for slide preparation using cover slips and slides, by placing the object to be observed on a sheet and performing observation in that state. Furthermore, when continuously observing the object placed on the sheet using an imaging device, a focus adjustment mechanism linked to the observation device is provided, making it possible to adjust the focus without adjusting the position of the light-receiving part on the imaging device. This makes it possible to quickly and easily prepare the object to be observed, and to perform operations related to improving the efficiency and accuracy of observation work during continuous observation of the object in a simple and reliable manner.

[0013] Furthermore, in one embodiment of the observation system of the present invention, the focus adjustment mechanism is characterized by comprising a sheet tension control means for controlling the tension of the sheet. When observing objects placed on a sheet, changes in the surrounding environment of the observation system may cause distortion or bending of the sheet. This distortion or bending of the sheet can cause a shift in the focus position of the imaging device. On the other hand, this feature allows for the control of the tension of the sheet on which the object to be observed is placed, in conjunction with the observation means. This corrects distortion and bending of the sheet during observation, smoothing the sheet and correcting focus misalignment. As a result, focus adjustment during observation becomes possible without operating the light-receiving part of the imaging means.

[0014] Furthermore, in one embodiment of the observation system of the present invention, the focus adjustment mechanism is characterized by comprising a sheet position adjustment means for adjusting the position of the sheet in the vertical direction. This feature allows for vertical adjustment of the sheet on which the object to be observed is placed, in conjunction with the observation means. This smooths the sheet and corrects any focus misalignment during observation. As a result, focus adjustment during observation can be performed without manipulating the light-receiving part of the imaging means.

[0015] Furthermore, in one embodiment of the observation system of the present invention, the focusing mechanism is characterized by comprising distance adjustment means for maintaining a constant distance between the object to be observed and the light-receiving part of the imaging means. This feature allows for continuous observation without causing any shift in focus, by maintaining a constant distance between the object being observed and the light-receiving unit of the imaging unit in conjunction with the observation unit. This makes it possible to adjust the focus during observation without manipulating the light-receiving unit of the imaging unit.

[0016] Furthermore, the focusing mechanism for the observation system of the present invention, which solves the above problems, is a focusing mechanism for an observation system comprising an imaging means for acquiring image data of an object to be observed, a sheet on which the object to be observed is placed, and an observation means for continuously observing a plurality of objects to be observed placed on the sheet using the imaging means, and is characterized by comprising a distance adjustment means for maintaining a constant distance between the object to be observed and the light-receiving part of the imaging means. This feature allows for continuous observation of objects placed on a sheet using an imaging device. By maintaining a constant distance between the object and the light-receiving unit of the imaging device in conjunction with the observation device, it becomes possible to perform continuous observation without causing focus shifts. This makes it quick and easy to prepare the object for observation, and allows for focus adjustment without adjusting the position of the light-receiving unit on the imaging device side. As a result, operations related to improving the efficiency and accuracy of continuous observation of objects can be performed simply and reliably.

Advantages of the Invention

[0017] According to the present invention, in the observation of an observation object, it is possible to provide an observation system and a focus adjustment mechanism of the observation system that can make the operation of preparing the observation object in a state where it can be observed quick and easy, and can simply and surely perform operations related to improving the observation work efficiency and observation accuracy.

Brief Description of the Drawings

[0018] [Figure 1] It is a schematic explanatory view of an observation system in a first embodiment of the present invention. [Figure 2] It is a schematic explanatory view showing another aspect of the observation system in a first embodiment of the present invention. [Figure 3] It is a schematic explanatory view of an observation system in a second embodiment of the present invention. [Figure 4] It is a schematic explanatory view of an observation system in a third embodiment of the present invention. [Figure 5] It is a schematic explanatory view showing another aspect of the observation system in a third embodiment of the present invention.

Modes for Carrying Out the Invention

[0019] Hereinafter, embodiments of an observation system and a focus adjustment mechanism of the observation system according to the present invention will be described in detail while referring to the drawings. Note that the observation system and the focus adjustment mechanism of the observation system described in the embodiments are merely examples for explaining the observation system and the focus adjustment mechanism according to the present invention, and are not limited thereto.

[0020] In the observation system of the present invention, the observation object to be observed is not particularly limited, and an object that requires accumulation and analysis of observation data by observation in various fields can be used as the observation object. Further, the form of the observation object may be any form that can be placed on a sheet, and may be in the form of a liquid or a solid, or a solution containing the observation object. Recent advances in medicine and biology have increased the importance of accumulating and analyzing observational data related to cells, microorganisms, and other organisms. Therefore, the objects of observation in this invention include cells, microorganisms, and other organisms for which the collection of observational data is considered important from a medical and biological perspective. In particular, it is preferable to use living organisms such as living cells and living microorganisms as objects of observation, which have been difficult to observe with conventional slide preparation methods. Other examples of objects of observation in this invention include powders and solid flakes of metals and rocks, as well as fine particles of organic and inorganic materials. In the following embodiments, we will mainly describe those in which an aqueous solution containing living organisms is used as the object of observation, but we are not limited to this.

[0021] [First Embodiment] (Observation system) Figure 1 is a schematic diagram illustrating the structure of an observation system in a first embodiment of the present invention. As shown in Figure 1, the observation system 1A in this embodiment comprises a first sheet 2a, a placement means 3 for arranging the object to be observed S, an imaging means 4 for acquiring image data of the object to be observed S, an observation means 5 for continuously observing a plurality of objects to be observed S, and a focus adjustment mechanism 6 for adjusting the focus of the imaging means 4. In Figure 1, the dashed arrows indicate connections that enable data input / output and control.

[0022] In this embodiment, the observation system 1A observes the object to be observed by placing the object to be observed S on the first sheet 2a using the placement means 3, and then acquiring image data using the imaging means 4 in this state. In other words, in this embodiment, the observation system 1A uses the first sheet 2a on which the object to be observed S is placed as the sample, instead of the conventional slide consisting of a cover slip and a glass slide. Furthermore, in order to observe the object to be observed continuously, an observation means 5 including a sheet moving mechanism 50 for moving the first sheet 2a is provided, making it possible to perform the entire process from sample preparation to sample observation as a series of operations. This simplifies the work of preparing and changing samples in conventional observations using slides, and improves the work efficiency related to observation.

[0023] The following describes in detail each component of observation system 1A.

[0024] (First sheet) The first sheet 2a is for placing and supporting the object to be observed S.

[0025] The first sheet 2a can be any sheet on which the object to be observed S can be placed and on which image data can be acquired (observed) by the imaging means 4, and is not particularly limited in terms of material or shape. The shape of the first sheet 2a can be such that it has a width that ensures a sufficient field of view for observation by the imaging means 4, which will be described later. Furthermore, the shape of the first sheet 2a can be, for example, a roll of film. This facilitates the movement of the sheet by the observation means 5 (sheet moving mechanism 50), which will be described later.

[0026] Examples of materials for the first sheet 2a in this embodiment include materials that are light-transmitting, insoluble in water, and impermeable to water. Resistance to organic solvents is even more preferable. Such materials include resin films and glass films. For example, using inexpensive resin films with high market supply, such as polyethylene film, polypropylene film, and polyethylene terephthalate (PET) film, can reduce the running costs associated with observation. Polyethylene film is known to have high terahertz wave transmittance and is particularly suitable for use when acquiring image data using an electron beam as the imaging means 4. Furthermore, it is preferable that the first sheet 2a is made of a material that exhibits low autofluorescence and absorbance in addition to light transmittance. This helps to suppress the influence on image data acquisition by the imaging means 4. Examples of such materials include resin films with low light absorption, such as cycloolefin films, or glass films. Glass films, in particular, are suitably used as a substitute for slides in optical observation. Autofluorescence and absorbance in the first sheet 2a may be addressed by correcting them as background during image data acquisition by the imaging means 4. Furthermore, the first sheet 2a is preferably resistant to various sterilization treatments (electron beam sterilization, ultraviolet sterilization, heat sterilization, etc.). This makes it possible to suppress contamination by bacteria and microorganisms other than the object of observation when a living organism is used as the object of observation S. Also, when a living organism is used as the object of observation S, the first sheet 2a may be selected or given oxygen permeability / impermeability. For example, if the living organism is anaerobic, it is preferable to select a material that is oxygen impermeable for the first sheet 2a. On the other hand, if the living organism is aerobic, it is preferable to select a material that is oxygen permeable for the first sheet 2a. This makes it possible to control the effect of oxygen on the living organism during observation.

[0027] Furthermore, the first sheet 2a may be subjected to a surface treatment. The type of surface treatment is not particularly limited. For example, surface treatments include hydrophilic and hydrophobic treatments, which relate to the surface properties with respect to water. Note that surface properties with respect to water include not only hydrophilic / hydrophobic properties, but also wetting properties and water contact angle. In this case, the hydrophilic or hydrophobic treatment may be applied to the entire sheet, but one approach is to give a certain pattern to the areas where the hydrophilic or hydrophobic treatment is applied. For example, the hydrophilic treatment is applied so that square, rectangular, circular, or elliptical patterns are arranged at regular intervals, and the observation object S, which is an aqueous solution, is placed on the hydrophilic treated areas using the placement means 3 described later. This makes it possible to stably place the observation object S on the surface treated areas.

[0028] Another example of surface treatment is applying a substance to immobilize the object to be observed S. For example, if the object to be observed S is a living organism, the substance applied to the first sheet 2a could be an antibody against the organism, or a protein, peptide, enzyme, nucleic acid, base, sugar chain, phospholipid, glycolipid, etc. that binds to the organism. This makes it possible to stably position the object to be observed S in the surface-treated area. Furthermore, another example of surface treatment is applying a reagent to the object S for observation. Examples of such reagents include staining reagents, fluorescent reagents, and various probes. This makes it easier to observe the object S in the treated area using the imaging means 4, thereby improving the efficiency of the observation process.

[0029] Furthermore, the first sheet 2a may incorporate various identification information to facilitate identification during observation. Examples of such identification information include size-related identification information such as grids and scale bars, and position-related identification information such as markers and codes (numbering, character codes, barcodes, etc.) placed at predetermined intervals. Means for incorporating the identification information include pre-printing the identification information on the first sheet 2a or manually writing it in.

[0030] (Arrangement means) The placement means 3 is for placing the object to be observed S on the first sheet 2a. The arrangement means 3 in this embodiment is not particularly limited, as long as it can accommodate the object to be observed S. It is preferable to select an arrangement means 3 that matches the form of the object to be observed S (liquid or solid).

[0031] A specific example of the arrangement means 3 is a system that includes a dispensing unit 30, a supply unit 31 that supplies the object to be observed S to the dispensing unit 30, and a control mechanism 32 that controls the angle and position of the dispensing unit 30, as shown in Figure 1.

[0032] The dispensing unit 30 is used when the object to be observed S is a liquid, and it drops and discharges the object to be observed S onto the first sheet 2a. The dispensing section 30 can be any structure suitable for dropping and dispensing liquid, such as a tip or syringe. The material of the dispensing section 30 is not particularly limited and can be selected according to the properties of the object to be observed S. Examples of materials for the dispensing section 30 include plastic, metal, and glass.

[0033] The dispensing unit 30 is preferably replaceable. This allows the dispensing unit 30 to be replaced according to the type of object S being observed or changes in observation conditions, thereby suppressing contamination such as the mixing of other objects S being observed during observation. Furthermore, from the viewpoint of suppressing contamination, the dispensing unit 30 should be washable and cleaned each time the type of object S being observed changes. From the viewpoint of improving the efficiency of observation work, it is preferable to make the dispensing unit 30 replaceable. In this case, an inexpensive plastic tip is used as the dispensing unit 30 and replaced on a disposable basis. This makes it possible to place the object to be observed S in a short time by only changing the tip, so that observation can be performed in a nearly continuous state. On the other hand, when a metal or glass tip or syringe is used as the dispensing unit 30, it is preferable to provide a means for cleaning the dispensing unit 30. This makes the dispensing unit 30 reusable and reduces the cost of replacing the dispensing unit 30.

[0034] The supply unit 31 supplies the object to be observed S to the dispensing unit 30. The supply unit 31 only needs to be capable of supplying the object to be observed S to the dispensing unit 30, and its specific structure is not particularly limited. In this embodiment, the supply unit 31 may include, for example, a storage unit 31a for storing the object to be observed S, and a supply line 31b and a pump 31c for supplying the object to be observed S to the dispensing unit 30, as shown in Figure 1.

[0035] The storage section 31a in the supply section 31 is not particularly limited and can be anything that can store the object to be observed S (liquid). Examples include a water tank, flask, beaker, and tube. When storing a solution containing the object to be observed S, a stirring mechanism may be provided in the storage section 31a. This improves the dispersibility of the object to be observed S in the solution and prevents only the solution without the object to be observed S from being supplied to the dispensing section 30.

[0036] The supply line 31b and pump 31c are not particularly limited, as long as they are capable of transferring the object to be observed S (liquid). For example, the supply line 31b can be a tube made of resin or metal. The pump 31c can be a pump that can accurately supply a predetermined volume, such as various metering pumps or pumps equipped with flow control valves. This makes it easier to set conditions for the placement of the object to be observed S, thereby improving the work efficiency related to observation.

[0037] The control mechanism 32 is for controlling the angle and position of the dispensing unit 30. The control mechanism 32 includes a support connected to the dispensing unit 30 and a drive mechanism that drives the support along the XYZ axis and the rotation axis. The drive mechanism may be operated manually, or a control unit that performs control by numerical input or automatic control by program may be provided. This makes it possible to adjust the angle and position of the dispensing unit 30 with respect to the first sheet 2a and to place the object to be observed S at any position on the first sheet 2a.

[0038] One example of position control of the dispensing unit 30 by the control mechanism 32 is to move the dispensing unit 30 to a position away from the first sheet 2a when the observation work is temporarily stopped and then resumed, such as during and immediately after cleaning the dispensing unit 30, or immediately after changing the type of object to be observed S. After a certain amount of liquid in the dispensing unit 30 is discharged as waste liquid, the dispensing unit 30 is moved onto the first sheet 2a and the object to be observed S is placed. This prevents waste liquid unsuitable for observation from being placed on the first sheet 2a, and improves the efficiency of the observation work.

[0039] The arrangement means 3 is not limited to the configuration shown in Figure 1. For example, multiple arrangement means 3 may be provided. If multiple arrangement means 3 are provided, the items placed on the first sheet 2a by the arrangement means 3 may include not only the object to be observed S, but also reagents necessary for observing the object to be observed S. For example, if the object to be observed S is a solution containing living organisms, staining reagents widely used for observing living organisms can be placed using the placement means 3. Furthermore, items other than the object to be observed S placed using the placement means 3 are not limited to reagents (staining reagents) necessary for observing the object to be observed S, but can also include chemical substances (reagents) for observing the effects of environmental changes on the object to be observed S. For example, if the object to be observed S is a solution containing living organisms, a solution with a high salt concentration or pH can be placed using the placement means 3 and mixed with the object to be observed S to observe the effects of salt concentration and pH on living organisms. This makes it possible to quickly and easily obtain observation data related to environmental changes on the object to be observed S.

[0040] Another example of the placement means 3 is to use dispensing equipment such as pipettes as the dispensing unit 30 and supply unit 31, and to manually place the objects to be observed S. This is a particularly useful method when the number of objects to be observed S is small, such as when considering the setting of observation conditions, such as the selection of the material and surface treatment conditions of the first sheet 2a. Alternatively, the placement means 3 may use a pipette or syringe pump that automates the process from aspirating to discharging the objects to be observed S. This makes it easier to automate the placement process, thereby improving the work efficiency related to observation. Furthermore, by performing position control with the control mechanism 32, it is possible to switch the object to be aspirated as the object to be observed S and to automatically and continuously switch between multiple objects to be observed S.

[0041] Another example of the placement means 3 is to provide a distribution unit equipped with a pickup device such as tweezers instead of the dispensing unit 30. This makes it possible to place the solid slice as the object to be observed S on the first sheet 2a. The pickup device in the distribution unit can be any device that can grasp and carry the solid slice, which is the object to be observed S. Examples include a device with a structure that grips the solid slice, like general tweezers, or a device with a suction structure, like suction tweezers.

[0042] (Imaging method) The imaging means 4 is for acquiring image data of the object to be observed S placed on the first sheet 2a. The imaging means 4 is not particularly limited and can be any means capable of acquiring image data as observation data of the object to be observed S. For example, it could be used to acquire images to obtain information related to the shape of the object to be observed S, or to acquire images to obtain information related to optical changes (such as color changes or the presence or absence of fluorescence) or temperature changes based on the reaction between the object to be observed S and a reagent. In this embodiment, the imaging means 4 can be configured as a microscope. In particular, the configuration of an optical microscope is preferred. This allows for the acquisition of a magnified image of the object S, enabling detailed observation of the object S based on the acquired image data.

[0043] The imaging means 4 includes an objective lens 40 and a reflector (light source) commonly used in observation with an optical microscope, and by replacing the eyepiece with an image acquisition device 41 (CCD camera, CMOS camera, etc.), automatic collection of image data is made possible. This makes it possible to improve the work efficiency related to the acquisition and analysis of image data. Figure 1 shows the main components of the imaging means 4, namely the objective lens 40 and the image acquisition device 41, and other components are omitted. In the observation system 1A of this embodiment, as shown in Figure 1, a first sheet 2a held by the sheet moving mechanism 50 of the observation means 5 is placed below the objective lens 40, and in order to observe the object to be observed S placed on the first sheet 2a, it is not essential to provide a stage like that of a typical optical microscope. In Figure 1, the structure of an upright microscope is shown with the objective lens 40 above the object to be observed S. However, the microscope is not limited to this, and an inverted microscope structure with the objective lens 40 below the object to be observed S may also be used. Furthermore, multiple objective lenses 40 may be provided to observe the object to be observed S from multiple directions.

[0044] It is preferable to organize and analyze the image data obtained through the objective lens 40 as needed. For example, as shown in Figure 1, the system may include a data processing unit 42 connected to the image acquisition device 41 for storing and analyzing the obtained image data. The data processing unit 42 includes a data storage unit and a data analysis unit, and is equipped with a processor such as a CPU that performs necessary calculations by inputting image data from the image acquisition device 41. This enables the creation of a database of observation data based on image data related to the object of observation S and the effective utilization of the data. The data processing unit 42 also performs calculations to detect the appropriate focus position from the acquired image data. This makes it easy to perform quick and accurate focus adjustment using the focus adjustment mechanism 6, which will be described later.

[0045] (Means of observation) Observation means 5 is for continuously observing multiple objects S placed on the first sheet 2a using imaging means 4. More specifically, observation means 5 is for sequentially moving multiple objects S placed on the first sheet 2a to positions where image data can be acquired by imaging means 4. Observation means 5 replaces the conventional slide consisting of a cover slip and a glass slide with a first sheet 2a on which multiple objects to be observed S are arranged, making it possible to perform sample preparation and sample observation as a single series of operations. This simplifies the sample preparation and replacement work that is required in conventional observations using slides, and improves the work efficiency for observations involving multiple objects to be observed S.

[0046] In this embodiment, the observation means 5 includes a sheet moving mechanism 50 that supports and moves the first sheet 2a. As shown in Figure 1, the sheet moving mechanism 50 includes an unwinding roll 51a as an unwinding section 51 for unwinding and supplying the first sheet 2a, and a winding roll 52a as a winding section 52 for winding up and recovering the first sheet 2a, and is provided with a drive unit (not shown) for rotating the winding section 52 (winding roll 52a).

[0047] The sheet moving mechanism 50 preferably has a speed adjustment function 53 for adjusting the sheet's moving speed. As an example of the speed adjustment function 53, a rotational drive control unit 53a is provided for the drive unit related to the rotational drive of the winding unit 52, which is capable of recording the number of rotations or measuring the winding diameter, and is also capable of controlling the number of rotations. This makes it possible to adjust the winding (recovery) speed of the sheet, and thus the sheet's moving speed.

[0048] In this embodiment, the sheet moving mechanism 50 may have a speed adjustment function 53 for adjusting the sheet's moving speed, as well as a function for controlling the sheet's movement and stopping. For example, the drive unit for the rotational drive of the winding unit 52 may be controlled to move the first sheet 2a a certain distance or for a certain period of time, and then stop it for a certain period of time. This control may be performed by the rotational drive control unit 53a, or a separate control unit may be provided. This makes it possible to improve the accuracy of observation by intermittently moving the object S when acquiring image data (observation) of the object S by the imaging means 4, and by performing observation while the object S is stationary. Furthermore, other examples of control related to sheet movement in the sheet movement mechanism 50 include switching between different operating modes, such as repeatedly moving and stopping the sheet as an operating mode for observing the object to be observed S, or moving the sheet at a constant speed as an operating mode for positioning the object to be observed S. This allows for more flexible setting of image data acquisition conditions in the imaging means 4, and enables more accurate positioning of the object to be observed S.

[0049] (Focus adjustment mechanism) The focus adjustment mechanism 6 works in conjunction with the observation means 5 to adjust the focus of the imaging means 4. In this embodiment, the focus adjustment mechanism 6 includes, for example, a tension control means 61 for controlling the tension of the first sheet 2a, as shown in Figure 1, and adjusts the focus position in the imaging means 4 by controlling the tension of the first sheet 2a in conjunction with the observation means 5.

[0050] When observing an object S placed on the first sheet 2a, distortion or bending may occur in the first sheet 2a due to changes in the surrounding environment of the observation system 1A. This distortion or bending of the first sheet 2a can cause a shift in the focus position of the imaging means 4. Therefore, by providing a tension control means 61 as the focus adjustment mechanism 6 and controlling the tension of the first sheet 2a in conjunction with the observation means 5, distortion and bending of the first sheet 2a during observation can be corrected, making the first sheet 2a smoother and correcting the focus position shift in the imaging means 4. This makes it possible to adjust the focus during image data acquisition (observation) without having to adjust the position of the light-receiving part that constitutes the imaging means 4. Furthermore, compared to having an autofocus function in the imaging means 4 that involves adjusting the position of the light-receiving part, etc., it is possible to save space for the observation system 1A as a whole.

[0051] The tension control means 61 in this embodiment only needs to have the function of appropriately controlling the tension of the first sheet 2a. For example, as shown in Figure 1, a rotational drive control unit 61a is provided that acquires information related to the focal position from the data calculation unit 42 of the imaging means 4 and controls the rotational speed of the drive unit related to the rotational drive of the winding unit 52 (winding roll 52a) based on this information related to the focal position. This allows the rotational speed of the winding unit 52 to be controlled and the tension of the first sheet 2a to be controlled so that the imaging means 4 can maintain an appropriate focal position when acquiring image data of the object to be observed S. In Figure 1, the rotational drive control unit 53a and the rotational drive control unit 61a are shown as separate components for the drive unit related to the rotational drive of the winding unit 52, but they may be integrated into a single unit. This will allow for space saving for the observation system 1A.

[0052] Other examples of tension control means 61 include a drive unit for rotational drive in the unwinding unit 51, or a rotational drive unit as a sheet feeding unit installed between the unwinding unit 51 and the dispensing unit 30, with a control unit capable of controlling the rotational speed of these drive units, and controlling the rotational speed based on information related to the focus position obtained from the data calculation unit 42. This enables more precise tension control. Another example of the tension control means 61 is a tension control mechanism that can apply a predetermined tension to the first sheet 2a. Such a tension control mechanism may, for example, use an elastic body and arrange it to apply a load directly or indirectly to the first sheet 2a. In this case, by appropriately selecting the type and performance of the elastic body used and adjusting the load applied to the first sheet 2a, the tension of the first sheet 2a can be controlled and an appropriate focus position can be maintained. When an elastic body is used as the tension control means 61, appropriate tension control of the first sheet 2a can be performed by a simple and inexpensive means without relying on the control of the rotation drive control unit 61a.

[0053] The tension control means 61 may perform tension control after understanding the tension in the first sheet 2a. For example, a tension detection unit may be provided between the unwinding unit 51 and the winding unit 52. This makes it possible to control the tension of the first sheet 2a more precisely, reduce distortion and bending of the first sheet 2a, and improve the accuracy of maintaining the focus position and observation.

[0054] (Another form of observation system) The observation system 1A in this embodiment is not limited to one that includes only the first sheet 2a as the sheet on which the object to be observed S is placed. Figure 2 is a schematic diagram illustrating another aspect of the observation system in the first embodiment of the present invention. Observation system 1A', which is an alternative embodiment of observation system 1A in this embodiment, includes a second sheet 2b that is superimposed on the first sheet 2a, and the imaging means 4 observes the object to be observed S fixed between the first sheet 2a and the second sheet 2b. Note that the same components as those of observation system 1A based on Figure 1 described above will be omitted from the explanation, and in Figure 2, some of the arrangement means 3 and the control means C described later will be omitted from the illustration.

[0055] In this embodiment, the observation system 1A' uses the placement means 3 to place the object to be observed S on the first sheet 2a, and then the second sheet 2b is placed on top of it, fixing the object to be observed S between the first sheet 2a and the second sheet 2b. In this state, image data is acquired (observed) by the imaging means 4. In other words, instead of a conventional slide consisting of a cover glass and a slide glass, the sample is made by stacking the first sheet 2a and the second sheet 2b in this embodiment, and observation is performed. Furthermore, by moving the first sheet 2a and the second sheet 2b using the observation means 5 (sheet moving mechanism 50), it becomes possible to perform the entire process from sample preparation to sample observation as a series of operations. This simplifies the sample preparation and replacement work in conventional observations using slides, and improves the efficiency of observation work.

[0056] In the observation system 1A' of this embodiment, the second sheet 2b is superimposed on the first sheet 2a on which the object to be observed S is placed, thereby fixing the object to be observed S in place.

[0057] The second sheet 2b can be made of the same material and shape as the first sheet 2a, and is not particularly limited in its details.

[0058] The first sheet 2a and the second sheet 2b may be made of the same material or different materials. Furthermore, the first sheet 2a and the second sheet 2b may have the same thickness or different thicknesses.

[0059] Furthermore, when surface treatment is performed on the first sheet 2a and the second sheet 2b, it is preferable that one or a part of either the first sheet 2a or the second sheet 2b have different surface properties with respect to water. This makes it easier for the aqueous solution containing the object to be observed S to spread appropriately between the sheets and maintain a constant distance between them when the second sheet 2b is placed on top of the first sheet 2a after the aqueous solution has been placed on top of the first sheet 2a. As a result, it becomes possible to stably fix the object to be observed S and to improve the work efficiency related to image data acquisition by the imaging means 4.

[0060] In the observation system 1A' of this embodiment, when the object to be observed S is a solution containing living organisms, it is preferable to provide multiple placement means 3 and to place staining reagents, which are widely used for observing living organisms, using the placement means 3. Furthermore, in this case, it is preferable that the object to be observed S and the staining reagent are mixed by overlapping the first sheet 2a and the second sheet 2b, rather than directly supplying the staining reagent to the object to be observed S placed on the first sheet 2a. This makes it possible to suppress contamination in the dispensing unit 30. For example, the object to be observed S and the staining reagent may be placed adjacent to each other on the first sheet 2a so that they do not come into contact with each other, or the angle of the dispensing unit 30 on which the object to be observed S is placed may be different from the angle of the dispensing unit 30 on which the staining reagent is placed, and the object to be observed S and the staining reagent may be placed on the first sheet 2a and the second sheet 2b, respectively.

[0061] By using the placement means 3 to place the object to be observed S on the first sheet 2a, and then overlapping the second sheet 2b from above, the object to be observed S can be fixed between the first sheet 2a and the second sheet 2b. The means for overlapping the first sheet 2a and the second sheet 2b are not particularly limited. For example, a sheet moving mechanism 50 can be used to overlap the first sheet 2a and the second sheet 2b. This makes it easier to automate the work related to sample preparation.

[0062] The imaging means 4 in the observation system 1A' of this embodiment is for acquiring image data relating to the object to be observed S fixed between the first sheet 2a and the second sheet 2b, and can be the same as that described in observation system 1A. In the observation system 1A' of this embodiment, as shown in Figure 2, two sheets (a first sheet 2a and a second sheet 2b) held by a sheet moving mechanism 50 are placed below the objective lens 40, and the object to be observed S fixed between the two sheets is observed. Therefore, similar to observation system 1A, it is not essential to provide a stage like that found in a typical optical microscope.

[0063] In the observation system 1A' of this embodiment, the observation means 5 (sheet moving mechanism 50) is for supporting and moving the first sheet 2a and the second sheet 2b. As shown in Figure 2, the sheet moving mechanism 50 includes an unwinding section 51 and a winding section 52, which consist of an unwinding roll 51a for unwinding and supplying the first sheet 2a, a winding roll 52a for winding and recovering the first sheet 2a, an unwinding roll 51b for unwinding and supplying the second sheet 2b, and a winding roll 52b for winding and recovering the second sheet 2b. A drive unit is provided (not shown) for rotating the winding section 52 (winding rolls 52a, 52b). Although Figure 2 shows a winding section 52 with winding rolls 52a and 52b, it is not limited to this. For example, the first sheet 2a and the second sheet 2b unwinded from the unwinding section 51 (unwinding rolls 51a, 51b) may be wound and recovered by a single winding roll. This makes it possible to simplify the structure of the device.

[0064] As the observation means 5 (sheet moving mechanism 50), it is preferable to provide a plurality of vertically movable support rollers 54a as a support mechanism 54 for overlapping the first sheet 2a and the second sheet 2b. This allows for stable movement of the first sheet 2a and the second sheet 2b. Furthermore, by providing the support mechanism 54, the accuracy of position adjustment related to the overlapping of the first sheet 2a and the second sheet 2b can be improved, and the distance between the two sheets can be easily adjusted. In Figure 2, the support mechanism 54 is shown to be provided on the second sheet 2b side, but it is not limited to this, and may be provided on the first sheet 2a side, or on both the first sheet 2a side and the second sheet 2b side. Also, there are no particular limitations on the location or number of support mechanisms 54 provided. For example, as shown in Figure 2, support mechanisms 54 may be provided on both the upstream and downstream sides in the direction of sheet movement, and on the upper side of the sheet (upper side of the second sheet 2b), with respect to the objective lens 40 of the imaging means 4, thereby enabling stable support of the sheet. Other examples include providing the support mechanisms 54 on the lower side of the sheet (downper side of the first sheet 2a) or on the side of each sheet where the object to be observed S is placed. Furthermore, the number of support mechanisms 54 may be reduced to simplify the structure.

[0065] The tension control means 61, which is the focus adjustment mechanism 6 in the observation system 1A' of this embodiment, has the function of appropriately controlling the tension of either one or both of the first sheet 2a and the second sheet 2b. For example, as shown in Figure 2, information related to the focus position is acquired from the data calculation unit 42 in the imaging means 4, and a rotation drive control unit 61b is provided that can control the rotation speed of the drive unit related to the rotation drive of the winding unit 52 (winding rolls 52a, 52b) based on this information related to the focus position. As a result, when acquiring image data of the object to be observed S, the rotation speed of the winding unit 52 (winding rolls 52a, 52b) can be controlled so that an appropriate focus position can be maintained in the imaging means 4, and the tension of the first sheet 2a and / or the second sheet 2b can be controlled. Furthermore, since the observation system 1A' in this embodiment fixes the object to be observed S between the first sheet 2a and the second sheet 2b, it is preferable to perform coordinated tension control between the two sheets rather than performing independent tension control for each sheet. For this reason, in order to perform more precise tension control, as shown in Figure 2, it is preferable to provide a drive unit related to rotational drive in the unwinding section 51 (unwinding rolls 51a, 51b), and to connect the rotational drive control unit 61b so that it can also control the rotational speed of these drive units. This makes it possible to improve the accuracy of focus adjustment.

[0066] In this embodiment, observation systems 1A and 1A' are configured to allow observation of an object S on a sheet, and by performing observation in this state, sample preparation and observation are possible without the need for slide preparation using cover slips and slides. Furthermore, when continuously observing the object on the sheet using the imaging means, a focus adjustment mechanism linked to the observation means is provided, making it possible to adjust the focus without adjusting the position of the light-receiving part on the imaging means. This makes it possible to quickly and easily prepare an object for observation, and to perform operations that improve the efficiency and accuracy of observation work during continuous observation of the object in a simple and reliable manner.

[0067] Furthermore, in this embodiment, observation systems 1A and 1A' may be equipped with various means and mechanisms to improve the work efficiency and accuracy of observation, in addition to the imaging means 4 for acquiring image data of the object to be observed S. For example, one means of acquiring observation data of the object S to be observed is to further provide optical analysis means, radiation analysis means, magnetic field analysis means, electron diffraction means, etc., for obtaining information based on various analyses of the object S to be observed.

[0068] Furthermore, it is preferable that the observation system 1A and observation system 1A' in this embodiment be provided with a control means C for adjusting various operations in the placement means 3, imaging means 4, observation means 5 (sheet moving mechanism 50), and focus adjustment mechanism 6. More specifically, as shown in Figure 1, a control unit C1 is provided that is connected to the placement means 3, imaging means 4, observation means 5 (sheet moving mechanism 50), and focus adjustment mechanism 6, enabling data input / output and control. A control unit C1 may be provided for each type of means, but as shown in Figure 1, it is preferable to connect multiple means to one control unit C1 to enable comprehensive control. This makes it possible to adjust various operations and perform operations related to adjustment in a simple and efficient manner. Here, examples of operations to be controlled by the control unit C1 include operations related to the operation of the imaging means 4, operations related to the acquisition and storage of image data by the imaging means 4, and operations related to the placement of the object to be observed S on the sheet (first sheet 2a) by drive control of the supply unit 31 and control mechanism 32 of the placement means 3 based on data related to the analysis results of the image data by the imaging means 4. Other examples include operations directly related to the horizontal movement of the sheet, such as control of the sheet's movement speed and movement / stopping of the sheet by the observation means 5 (sheet moving mechanism 50), and sheet operations such as tension control of the sheet by the focus adjustment mechanism 6 (tension control means 61) that are related to maintaining the focus position. Furthermore, it is preferable to control not only individual operations of each means, but also operations resulting from combinations of operations of various means. For example, this could involve controlling a series of operations, such as acquiring image data by the imaging means 4 at the same time the sheet movement mechanism 50 stops moving the sheet, or controlling a series of operations related to the placement means 3, imaging means 4, observation means 5 (sheet movement mechanism 50), and focus adjustment mechanism 6 for each of multiple observation objects S. This makes it possible to improve the accuracy of the observation data acquired as observation system 1A (or observation system 1A') and to improve the work efficiency related to observation.

[0069] [Second Embodiment] Figure 3 is a schematic diagram illustrating the observation system in a second embodiment of the present invention. The observation system 1B according to the second embodiment is equipped with a sheet position adjustment means 62 for adjusting the sheet position vertically, in place of the tension control means 61 which serves as the focus adjustment mechanism 6 in the observation system 1A' of the first embodiment. Note that the same components as those in the first embodiment will not be described.

[0070] As shown in Figure 3, the observation system 1B in this embodiment is provided with a sheet position adjustment means 62 as a focusing mechanism 6 that moves the first sheet 2a and / or the second sheet 2b in the vertical direction, and by adjusting the position of the sheet relative to the imaging means 4, the sheet is smoothed and an appropriate focus position is maintained. As an example of the seat position adjustment means 62, a support mechanism 54 provided for overlapping the first seat 2a and the second seat 2b may be used. As described above, multiple vertically movable support rollers 54a are provided to stably move the first sheet 2a and the second sheet 2b. Therefore, as a sheet position adjustment means 62, a drive control unit 62a is provided that acquires information related to the focal position from the data calculation unit 42 of the imaging means 4 and controls the position of the vertical movement mechanism of the support rollers 54a based on this focal position information. This allows the position of the support mechanism 54 to be controlled and the position of the sheet (second sheet 2b in Figure 3) to be adjusted so that the imaging means 4 can maintain an appropriate focal position when acquiring image data of the object to be observed S. In Figure 3, the support mechanism 54 is shown to be provided on the second sheet 2b side, but it is not limited to this. It may also be provided on the first sheet 2a side, or on both the first sheet 2a side and the second sheet 2b side. Furthermore, there are no particular limitations on the location or number of support mechanisms 54 provided. In this case, the seat position adjustment means 62 only needs to be able to move the seat position up and down using the support mechanism 54. Therefore, the seat position adjustment means 62 may be provided in proportion to the number of support mechanisms 54, or it may be provided for some of the support mechanisms 54.

[0071] Furthermore, the support mechanism 54 may also be equipped with a function to adjust the sheet's movement speed in addition to the vertical movement mechanism. For example, it may be provided with a measuring unit that measures the number of rotations or rotational speed of the support roller 54a and a rotational drive control unit that can control the rotational drive of the support roller 54a. The first sheet 2a and the second sheet 2b move in conjunction with the rotational drive of the support roller 54a. Therefore, in addition to the speed adjustment function 53, the sheet's movement speed can also be adjusted by controlling the rotational drive of the support roller 54a.

[0072] The observation system 1B in this embodiment is not limited to one comprising a first sheet 2a and a second sheet 2b. For example, even in the case of an observation system 1A in the first embodiment, which is equipped only with a first sheet 2a, it is possible to maintain an appropriate focus position by providing a support mechanism that is vertically movable and has a function to support the first sheet 2a, and by performing similar position control with the sheet position adjustment means 62.

[0073] [Third Embodiment] Figure 4 is a schematic diagram illustrating the observation system in a third embodiment of the present invention. The observation system 1C according to the third embodiment includes a distance adjustment means 63 that maintains a constant distance between the object to be observed S and the light-receiving unit 40a of the imaging means 4, instead of the tension control means 61 which serves as the focus adjustment mechanism 6 in the observation system 1A' of the first embodiment. Note that the same components as those in the first embodiment will not be described.

[0074] In this embodiment, the observation system 1C acquires image data by fixing the focus position itself by maintaining a constant distance between the object to be observed S placed on the first sheet 2a and the light-receiving unit 40a of the imaging means 4. This makes it possible to perform continuous observation without causing any shift in the focus position in the first place.

[0075] In this embodiment, the distance adjustment means 63, which is the focus adjustment mechanism 6, only needs to be such that the distance between the object to be observed S, which is placed between the first sheet 2a and the second sheet 2b, and the light-receiving unit 40a of the imaging means 4 is kept constant. For example, as shown in Figure 4, one example of the distance adjustment means 63 is to provide a positioning jig 63a between the second sheet 2b and the light-receiving part 40a (the tip of the objective lens 40) of the imaging means 4. One example of a jig 63a is a structure that has space to secure the imaging field of view by the imaging means 4 (the observation field of view by the objective lens 40). More specifically, one example is a structure consisting of a cylindrical member or a frustoconical member having a cavity in the center. Another example of the jig 63a is a structure in which a light-transmitting material such as glass is embedded in at least the center.

[0076] The jig 63a is placed between the light-receiving unit 40a and the second sheet 2b. At this time, by setting the height of the jig 63a to correspond to the appropriate focus position of the imaging means 4 for acquiring image data of the object to be observed S, continuous observation becomes possible without any shift in focus position during observation by the observation means 5.

[0077] The means of positioning the jig 63a are not particularly limited. For example, the jig 63a may be placed stationary between the light-receiving unit 40a and the second sheet 2b, but it is more preferable to provide the jig 63a with a structure that allows it to be attached to the light-receiving unit 40a (objective lens 40). This ensures that the position of the jig 63a itself is more reliably fixed during continuous observation by the observation means 5, making it possible to maintain a stable and constant distance between the object to be observed S and the light-receiving unit 40a in the imaging means 4.

[0078] Furthermore, the configuration of the jig 63a in this embodiment can be made independent as a focus adjustment mechanism for the observation system according to the present invention. This focus adjustment mechanism can be applied to existing observation systems that continuously observe objects placed on a sheet using an imaging means. This makes it quick and easy to prepare the objects to be observed, and allows for focus adjustment without adjusting the position of the light-receiving unit on the imaging means side, thereby enabling simple and reliable operation to improve the efficiency and accuracy of observation work in continuous observation of objects.

[0079] Another example of the distance adjustment means 63 is to set the distance between the light-receiving unit 40a of the imaging means 4 and the object to be observed S to approximately zero distance without using the jig 63a. Figure 5 is a schematic diagram illustrating another aspect of the observation system in the third embodiment of the present invention. Observation system 1C', an alternative embodiment of observation system 1C in this embodiment, uses an image sensor (micro-imager, image sensor) that does not use a lens (objective lens 40) instead of an optical microscope as the imaging means 4. By using an image sensor as the image acquisition device 41 of the imaging means 4, the focal position becomes directly above (directly below in Figure 5) the light-receiving unit 40a. Therefore, by positioning the light-receiving unit 40a of the imaging means 4 to be in contact with the second sheet 2b from above the second sheet 2b, the distance between the light-receiving unit 40a of the imaging means 4 and the object to be observed S is made approximately zero, making it possible to observe continuously without any shift in the focal position during observation by the observation means 5. In the observation system 1C' shown in Figure 5, the object to be observed S is shown fixed between the first sheet 2a and the second sheet 2b, but the system is not limited to this. For example, only the first sheet 2a may be used, and the light-receiving unit 40a of the imaging means 4 may be positioned below the first sheet 2a so that it is in contact with the first sheet 2a, thereby making the distance between the light-receiving unit 40a of the imaging means 4 and the object to be observed S approximately zero.

[0080] The embodiments described above are examples of observation systems and focusing mechanisms for observation systems. The observation system and focusing mechanism for observation systems according to the present invention are not limited to the embodiments described above, and the observation system and focusing mechanism for observation systems according to the embodiments described above may be modified without changing the gist of the claims.

[0081] For example, the observation system in this embodiment may include a retrieval means for retrieving the object to be observed S after observation by the imaging means 4 and the observation means 5. The retrieval means is not particularly limited and can be any means capable of retrieving the object to be observed S. One example of a retrieval method is to provide a means for removing the object to be observed S that was fixed between the first sheet 2a and the second sheet 2b after they have been separated. Another example of a retrieval method is to provide a means for cutting the sheets while the object to be observed S is fixed between the first sheet 2a and the second sheet 2b. By recovering the observed object S after observation, it becomes possible to preserve the object S as a specimen along with the observation data. This is especially useful for recovering objects S that are rare or have high specimen value, such as living organisms. Furthermore, the recovered objects S can be used as samples to supplement the database of observation data, or for the reuse of the objects S themselves.

[0082] Furthermore, regarding the supply and retrieval of sheets in the observation system of this embodiment, a sheet supply and retrieval mechanism other than having unwinding rolls 51a and 51b as an unwinding unit 51 for unwinding and supplying the first sheet 2a and the second sheet 2b, and winding rolls 52a and 52b as a winding unit 52 for winding and retrieving the first sheet 2a and the second sheet 2b may also be provided. An example of such a sheet supply and retrieval mechanism is a cartridge type in which the functions related to sheet supply and retrieval are housed in a casing. By using a cartridge type for the sheet supply and retrieval mechanism, sheet replacement and storage become easier. In addition, because the sheet is inside the casing, it becomes easier to suppress the contamination and adhesion of foreign matter to the sheet before supply or after retrieval during observation or sheet movement. [Industrial applicability]

[0083] The observation system and the focusing mechanism of the observation system of the present invention can be used for observing various objects. In particular, it is preferably used for observing living organisms. [Explanation of Symbols]

[0084] 1A, 1A′, 1B, 1C, 1C′ Observation system, 2a First sheet, 2b Second sheet, 3 Placement means, 30 Dispensing unit, 31 Supply unit, 31a Storage unit, 31b Supply line, 31c Pump, 32 Control mechanism, 4 Imaging means, 40 Objective lens, 40a Light receiving unit, 41 Image acquisition device, 42 Data calculation unit, 5 Observation means, 50 Sheet moving mechanism, 51 Unwinding unit, 51a, 51b Unwinding roll, 52 Winding unit, 52a, 52b Winding roll, 53 Speed ​​adjustment function, 53a Rotation drive control unit, 54 Support mechanism, 54a Support roller, 6 Focus adjustment mechanism, 61 Tension control means, 61a, 61b Rotation drive control unit, 62 Sheet position adjustment means, 62a Drive control unit, 63 Distance adjustment means, 63a Jig, C control means, C1 control unit, S object to be observed

Claims

1. An imaging means for acquiring image data of an object to be observed, A sheet on which the object to be observed is placed, An observation means comprising a sheet moving mechanism for moving the sheet, and moving a plurality of objects to be observed placed on the sheet and continuously observing them with the imaging means, An observation system characterized by comprising a focus adjustment mechanism that adjusts the focus of the imaging means by controlling the sheet moving mechanism of the observation means based on information from the imaging means.

2. The observation system according to claim 1, characterized in that the focus adjustment mechanism includes a sheet tension control means for controlling the tension of the sheet.

3. The observation system according to claim 1, characterized in that the focus adjustment mechanism includes a sheet position adjustment means for adjusting the position of the sheet in the vertical direction.

4. The observation system according to claim 1, characterized in that the focus adjustment mechanism includes a distance adjustment means for maintaining a constant distance between the object to be observed and the light-receiving part of the imaging means.

5. An imaging means for acquiring image data of an object to be observed, A sheet on which the object to be observed is placed, A focusing mechanism for an observation system comprising: an observation means for continuously observing a plurality of objects to be observed arranged on the sheet using the imaging means, The system includes a distance adjustment means for maintaining a constant distance between the object to be observed and the light-receiving unit of the imaging means. The distance adjustment means is provided between the sheet and the light-receiving unit of the imaging means. A focusing mechanism for an observation system, characterized by being a jig consisting of a structure having space to secure an imaging field of view, or a structure having a light-transmitting material in its center.

Images