Qualitative and quantitative determination of single viral haplotypes in composite samples.

SMRT sequencing with UMI workflow enhances the detection and quantification of revertant variants in attenuated dengue virus samples, overcoming limitations of prior methods by accurately identifying multiple haplotypes and reducing sequencing error rates.

JP7870255B2Active Publication Date: 2026-06-04TAKEDA VACCINES INC
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
TAKEDA VACCINES INC
Filing Date
2021-04-09
Publication Date
2026-06-04

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Abstract

The present invention provides methods for detecting the presence of at least one revertant virus in a sample containing an attenuated virus by single molecule real-time (SMRT) sequencing. The present invention further provides methods for the quantitative analysis of a mixture of viral haplotypes in a viral sample by SMRT sequencing. The present invention also provides kits useful for carrying out the methods.
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