Polarizing agent for DNP-NMR, method for producing the same, and method of use.

Abstract

Provided are polarizing agent with which it is possible to selectively enhance an NMR signal by using a DNP-NMR method even in a reductive environment, and a method for selectively enhancing an NMR signal.　The present invention is a polarizing agent used in a DNP-NMR method, the polarizing agent containing nanodiamond particles, and the surfaces of the nanodiamond particles being modified using a functional group capable of covalently bonding with or interacting with proteins. This polarizing agent makes it possible to selectively enhance an NMR signal by using a DNP-NMR method even in a reductive environment.

Claims

1. A polarizer used in the DNP-NMR method, Contains nanodiamond particles, The nanodiamond particles are a DNP-NMR polarizer, wherein their surface is modified with at least one functional group selected from the group consisting of -C=C-, -COOH, -NH₂, -S-S-Py (where Py represents a pyridine ring), and biotin-derived groups.

2. The DNP-NMR polarizer according to claim 1, wherein the nanodiamond particles are further modified with charged groups.

3. A DNP-NMR polarizer according to claim 1 or 2, comprising the functional group and a protein bound together.

4. A method for producing a DNP-NMR polarizer according to claim 1 or 2, A method for producing a compound, comprising the step of modifying it with a compound having the aforementioned functional group.

5. A method for using a DNP-NMR polarizer according to claim 1 or 2, A method of use comprising enhancing the NMR signal by binding a target protein to the aforementioned functional group.

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